Acta morphologica Hungarica最新文献

Methylglyoxal-induced morphological changes were studied in cultured rat hepatocytes. Hepatocytes incubated either in the presence or absence of 2.5 mM Ca2+ were injured by 10 mM methylglyoxal to a similar extent, while 1 mM of the alpha-oxoaldehyde caused a moderate damage only in the absence of Ca2+. In the absence of Ca2+, however, hepatocytes were already injured, even without the presence of methylglyoxal hepatotoxicity proceeds independently of the Ca2+ influx.

Topographic distribution of terminal and intercalary carbohydrate moieties were studied using horseradish peroxidase conjugated Glycine max (GMA), Arachis hypogea (PNA), Lotus tetragonolobus (LTA), Bandirarea simplicifolia (BSA), and Dolichos biflorus (DBA) agglutinins. N-Gal NAc, alpha-D-Gal, alpha-L-Fuc, D-Gal (beta-1-3)-D-Gal NAc, and alpha-beta-D Gal NAc linked glyco-conjugates were exposed during follicular atresia in oocyte, zona pellucida and granulosa cells. Theca interna and interstitial gland tissue revealed homology in lectin binding.

A new computerized polarization microscopic method was developed for objective, quick quantitative analysis of anisotropic structures. The paper presents the technology on collagen fibers of kidney and liver preparations. According to the observations the birefringence of one fiber can be characterized not by a single number as it is accepted generally but by a series of data. It suggests that the process of compensation is gradual. One of the explanations of this fact might be that the collagen fibers are inhomogeneous at submicroscopic level. With regard to this assumption, first several birefringent capillary basement membranes of a whole glomerulum were measured simultaneously. In this case, the individual capillaries showed nearly the same retardation. Second, portal zones of normal and cirrhotic livers as functional units were analyzed simultaneously. In the latter case the individual fibers show different birefringence. Comparing the results obtained by their technique with the data of the classic measuring method authors found similarity that proved its validity. Data suggest that the new method seems to be useful in experimental and diagnostic pathology.

A population-based and validated data set of 14 cases with isolated proximal intercalary type of congenital limb deficiency born in Hungary between 1975-84 was evaluated. Two cases had phocomelia of upper limbs, while 12 cases were affected with classical intercalary defects mainly in femurs. Of 14 cases, 13 had unimelic manifestation and both sexes were equally affected. The intrauterine growth retardation, the excess of second birth order, a higher rate of acute maternal disorder of the respiratory system and the lack of familial cluster are noteworthy. The vascular disruption hypothesis seems to be the most plausible explanation for the origin of isolated proximal intercalary defects.

Authors describe a new mathematical approach based on mixture decomposition and maximum likelihood method for the determination of subpopulations and G1-S-G2 phase fraction ratios in aneuploid, multiploid DNA histograms. A special computer routine was developed for this purpose (DAHSY). The present study relates to DNA histograms obtained by a Leitz Miamed DNA TV image analyser system. Altogether 23 normal, 14 dysplastic and 28 tumours Feulgen stained gastric imprint smears were measured. In addition to determination of subpopulations and G1-S-G2 phase fraction ratios, the authors also performed a morphometric analysis of Feulgen stained nuclei and determined the values of 5c exceeding rate (5cER) and the 2c deviation index (2cDI). More than one subpopulation were found only in tumours. The usual G1-S-G2 phase fraction rates in normal gastric smears were 85.5%, 9.04%, 4.9%, in dysplastic cases 74%, 15.4%, 10.2% and in tumours 53.8%, 22.9%, 18.8%. Significance of the differences were calculated by the T-test. The G1 phase fraction ratio clearly discriminates the malignant lesions from all others. The S+G2 phase fraction ratio, however, distinguishes the normal cases from the dysplastic ones. The 2cDi and 5cEr could support a multivariate diagnostic system. The cell nucleus area was found also to be of diagnostic value.

The authors report two cases of a peculiar microphlebitis of the intestines, similar to that described by Saraga and Costa quite recently [5]. The patients had undergone hemicolectomy because of evolving ileus caused by cecal polyps or lipohyperplasia, respectively. Pseudomembranous-ulcerative inflammation of the cecum and variously intense lymphocytic infiltrates of numerous small submucosal veins and venules of the intestines were found in both cases. Thrombosis occurred very rarely in the affected vessels, although sometimes it was found in deeper and larger veins. Arteries, lymphatics, mesenterial veins and lymph nodes were normal. Parts of the distal ileum and ascending colon displayed the phlebitic changes without mucosal alterations. The authors hypothesize that it was not the abnormal local circulation, but some hitherto not fully clarified immunological disorder that resulted in the disease. In contrast to the claim of Saraga and Costa [5], it is suggested that thrombosis of the small veins does not have a significant role in the development of the lesions, but a complex process that includes the entry of antigens via the altered mucosa followed by an immunogenic inflammatory response of the small veins is responsible for the pathogenesis.

We reviewed the autopsy material of 169 patients with rheumatoid arthritis (RA) and studied the cardiac changes is these patients. Systemic vasculitis was observed in 26 cases (15.38%) among 169 patients with RA. In 17 cases (10%) we found vasculitis of the subepicardial and/or intramural coronary vessels. Coronary arteritis or arteriolotis has led to multifocal small, and/or large myocardial infarctions in 10 cases (5.9%) and was the cause of progressive cardiac insufficiency, the direct cause of death. Multifocal circumscribed myocardial infarction reported in rheumatoid disease. In 8 cases rheumatoid nodules were found in the myocardium, 3 of them related to vasculitis. We suggest that rheumatoid nodules are the most severe form of necrotizing granulomatous vasculitis. Pathognomic nodular rheumatoid pericarditis was seen in 3 cases and diffuse rheumatoid pericarditis in another case. Pathognomic nodular valvulitis was found in 7 cases. Rheumatoid nodules localized to the epi-, myo-, or endocardium were observed on 9 patients. Generalized secondary amyloidosis was observed in 32 (18.93%) of the 169 patients with RA. Secondary amyloidosis was prevalent in the heart in 29 of 32 cases (relative frequency: 90.62%).

The effects of intravesical therapy with adriamycin, 1.6-dibromo-1.6-dideoxydulcitol (DBD) or with 1.6-dianhydrodulcitol (DAD) on bladder carcinogenesis were investigated in rats. To induce premalignant lesions in the urinary bladder female Sprague-Dawley rats received 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in their drinking water for 4 weeks and then post-treated intravesically with one of the antitumor drugs and examined once a week for 3 months. These antitumor drugs in healthy rats (i.e. without the pre-administration of BBN) did not cause any significant morphological changes in the urinary bladder after intravesical application once a week for 3 months. In the applied dose BBN alone induced only premalignant lesions in the urinary bladder. However, neoplastic lesions occurred in the groups treated with BBN and adriamycin (9 papillomas and 3 carcinomas in 14 animals). Similarly intravesical application of DBD after BBN administration resulted 5 carcinomas among the 11 animals. On the contrary no urinary bladder tumor was found in the animals treated with BBN and DAD. As DAD is one of the conversion products of DBD it is conceivable that the difference between DBD and DAD action may be due to the formation of other solvolytic product from DBD than DAD.

Sheep kidneys were injected with polyester crystic (Dewilux 511-0196) and three dimensional forms of the structure forming the postglomerular microcirculation were examined by scanning electron microscope. During the examination of the afferent and efferent arterioles, a glomerulus with double efferent arterioles were examined and microdiverticular structures were observed in the terminal end of peritubular capillary plexus. It is concluded that these structures widen the surface area of the capillaries as a result of which diffusion across the capillary bed is facilitated.