{"title":"Research Progress of Stirring System in Mammalian Cell Culture","authors":"梦娇 周","doi":"10.12677/bp.2023.131002","DOIUrl":"https://doi.org/10.12677/bp.2023.131002","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87609153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Role of Veterinarians in High-Level Biosafety Animal Laboratory Activities","authors":"家红 高","doi":"10.12677/bp.2023.131004","DOIUrl":"https://doi.org/10.12677/bp.2023.131004","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77279322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This experiment made the identification of the morphological characteristics, physical and chemical characteristics and the molecular biology to QSM01, QSM02, QSM03 which are isolated from a disease Quasipaa spinosa in meningitis and at the same time, made the drug screening, rapid PCR diagnostic techniques and immune prevention tests. The result showed QSM01 is Chryseobacterium joostei ; QSM02 is Elizabethkingia meningoseptica ; QSM03 is Chryseobacterium scophthalmum . Drug sensitivity test showed that QSM01 was sensitive to 5 antibiotics such as rifampicin and moderately sensitive to 5 chloramphenicol such as Chloramphenicol, QSM02 strain was sensitive to erythromycin, vancomycin and rifampicin, moderately sensitive to chloramphenicol, streptomycin and vancomycin, QSM03 strain was sensitive to 6 antibiotics such as vancomycin, moderately sensitive to 5 antibiotics such as tetracycline. The duplex PCR can detect the heaviest pathogenic bacterial strain QSM02 specifically. The immune protection rate for Quasipaa spinosa after injection, soak and spray of QSM02 inactivated vaccine can reach 100%, 87.5%, 75%. Therefore, it’s feasible to control the disease by soaking and spraying the vaccine.
{"title":"Meningitis Pathogenic Bacteria Identification, Doublex PCR Rapid Diagnosis and Immune Protective Effect of the Vaccine in the Frog (Quasipaa spinosa)","authors":"晓峰 黄","doi":"10.12677/bp.2023.131009","DOIUrl":"https://doi.org/10.12677/bp.2023.131009","url":null,"abstract":"This experiment made the identification of the morphological characteristics, physical and chemical characteristics and the molecular biology to QSM01, QSM02, QSM03 which are isolated from a disease Quasipaa spinosa in meningitis and at the same time, made the drug screening, rapid PCR diagnostic techniques and immune prevention tests. The result showed QSM01 is Chryseobacterium joostei ; QSM02 is Elizabethkingia meningoseptica ; QSM03 is Chryseobacterium scophthalmum . Drug sensitivity test showed that QSM01 was sensitive to 5 antibiotics such as rifampicin and moderately sensitive to 5 chloramphenicol such as Chloramphenicol, QSM02 strain was sensitive to erythromycin, vancomycin and rifampicin, moderately sensitive to chloramphenicol, streptomycin and vancomycin, QSM03 strain was sensitive to 6 antibiotics such as vancomycin, moderately sensitive to 5 antibiotics such as tetracycline. The duplex PCR can detect the heaviest pathogenic bacterial strain QSM02 specifically. The immune protection rate for Quasipaa spinosa after injection, soak and spray of QSM02 inactivated vaccine can reach 100%, 87.5%, 75%. Therefore, it’s feasible to control the disease by soaking and spraying the vaccine.","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83984932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, the development of spatial transcriptomics has enabled multiple analyses of cell transcriptome and its spatial location. With the increasing ability and efficiency of experimental technology, the requirement of developing analytical methods has gradually emerged. Techniques for generating Spatially Resolved Transcriptome (SRT) data are rapidly improving and being applied to study a variety of biological tissues. It is critical to study how spatially localized gene ex-王琳，赵桂华
{"title":"Statistical Methods for Spatially Re-solved Transcriptomic Data Analysis","authors":"琳 王","doi":"10.12677/bp.2023.131008","DOIUrl":"https://doi.org/10.12677/bp.2023.131008","url":null,"abstract":"In recent years, the development of spatial transcriptomics has enabled multiple analyses of cell transcriptome and its spatial location. With the increasing ability and efficiency of experimental technology, the requirement of developing analytical methods has gradually emerged. Techniques for generating Spatially Resolved Transcriptome (SRT) data are rapidly improving and being applied to study a variety of biological tissues. It is critical to study how spatially localized gene ex-王琳，赵桂华","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90190631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article describes the infection factors, pathogenicity, epidemiology, transmission routes, incubation period, physical inactivation, susceptibility and preventive treatment of H1N1, studied the methods of risk identification, risk analysis, risk control and risk evaluation of H1N1. The risks posed by experimental samples, experimental contents, facilities and equipment, misused/malicious use and accidents/incidents, and external activities to H1N1 were analyzed, presented conclusions such as the recommendation to classify H1N1 as an important animal infectious disease in import.
{"title":"Risk Assessment of Influenza A H1N1 Virus in Laboratory Activities","authors":"斌 吴","doi":"10.12677/bp.2023.133018","DOIUrl":"https://doi.org/10.12677/bp.2023.133018","url":null,"abstract":"This article describes the infection factors, pathogenicity, epidemiology, transmission routes, incubation period, physical inactivation, susceptibility and preventive treatment of H1N1, studied the methods of risk identification, risk analysis, risk control and risk evaluation of H1N1. The risks posed by experimental samples, experimental contents, facilities and equipment, misused/malicious use and accidents/incidents, and external activities to H1N1 were analyzed, presented conclusions such as the recommendation to classify H1N1 as an important animal infectious disease in import.","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78380774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To analyze the genetic characteristics of the gene carrying rate and gene mutation type of thalassemia in reproductive age population in Kunming. Methods: High-throughput sequencing technology was used to detect thalassemia genes in women of reproductive age and their spouses
{"title":"Analysis of Gene Test Results of Thalassemia in Reproductive Age Population in Kunming","authors":"彬 李","doi":"10.12677/bp.2023.132010","DOIUrl":"https://doi.org/10.12677/bp.2023.132010","url":null,"abstract":"Objective: To analyze the genetic characteristics of the gene carrying rate and gene mutation type of thalassemia in reproductive age population in Kunming. Methods: High-throughput sequencing technology was used to detect thalassemia genes in women of reproductive age and their spouses","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79532878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To analyze the correlation of sleep disorders in patients with stroke. Methods: Selection of 205 cases for the period July 2021 to February 2023, the cases were from the Second People’s Hospital Affiliated to Fujian Traditional Chinese Medicine University treated patients with acute
{"title":"Study on Correlation between Stroke Lesion Site and Sleep Disorder","authors":"佳茜 唐","doi":"10.12677/bp.2023.132017","DOIUrl":"https://doi.org/10.12677/bp.2023.132017","url":null,"abstract":"Objective: To analyze the correlation of sleep disorders in patients with stroke. Methods: Selection of 205 cases for the period July 2021 to February 2023, the cases were from the Second People’s Hospital Affiliated to Fujian Traditional Chinese Medicine University treated patients with acute","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"22 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87645794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
High-throughput sequencing technology is called large-scale parallel sequencing technology, and the ideal practical strains can be obtained quickly by using an efficient high-throughput sampling method and sequencing method. For the application of the high-throughput sequencing method in bac-*
{"title":"Application and Development of High-Throughput Sequencing Technology in Environmental Microbiology","authors":"欣宇 吕","doi":"10.12677/bp.2023.132012","DOIUrl":"https://doi.org/10.12677/bp.2023.132012","url":null,"abstract":"High-throughput sequencing technology is called large-scale parallel sequencing technology, and the ideal practical strains can be obtained quickly by using an efficient high-throughput sampling method and sequencing method. For the application of the high-throughput sequencing method in bac-*","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86002689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil salinization is a major problem facing agricultural development. It is difficult to improve the soil and spend more, and it is difficult to solve the problem of large-scale agricultural products. Studying the salt resistance mechanism of plant provides theoretical basis for the development of salt-resistant plants, and plant hormones play an important
{"title":"Response of ABA and JA to Salt Stress in Plants","authors":"佳敏 王","doi":"10.12677/bp.2023.131005","DOIUrl":"https://doi.org/10.12677/bp.2023.131005","url":null,"abstract":"Soil salinization is a major problem facing agricultural development. It is difficult to improve the soil and spend more, and it is difficult to solve the problem of large-scale agricultural products. Studying the salt resistance mechanism of plant provides theoretical basis for the development of salt-resistant plants, and plant hormones play an important","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79116745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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