{"title":"Comparison of Effects of Different Brands of cf-DNA Preservation Tubes","authors":"燕虹 李","doi":"10.12677/bp.2021.114016","DOIUrl":"https://doi.org/10.12677/bp.2021.114016","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84635831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on the Detection of rs9263726 Locus by Nested PCR-RFLP","authors":"钟 柳英","doi":"10.12677/bp.2021.113006","DOIUrl":"https://doi.org/10.12677/bp.2021.113006","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82941068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Secretion is an attractive production mode for proteins that require posttranslational modifications carried out in the secretory pathway. For example, amino acid chains fold properly, disulfide bonds form correctly, and glycosylation occurs accurately as the protein is secreted. In addition, recovery of secreted proteins is simplified by the fact that cells need not be broken and the product may be a major species present in a minimal synthetic culture medium. The current state of the art permits the production and secretion of proteins by heterologous cells. While future efforts will be required to improve the efficiency of secretion, current methods yield commercially interesting levels of secretion from E. coli, B. subtilis, S. cerevisiae, Aspergilli, and many mammalian cell types. Each of these systems offers certain advantages, and the choice of system depends on the specific protein to be secreted. High-value human therapeutic products such as plasminogen activators are reasonable candidates for secretion from mammalian cell lines, while industrial proteins such as calf prochymosin require a more economical host, such as baker's yeast or Aspergillus. The wide variety of posttranslational modifications observed in nature may prevent any single secretion system from dominating the field for many years to come.
{"title":"Protein secretion systems in microbial and mammalian cells.","authors":"D. Moir, J. Mao","doi":"10.1201/9781003066392-5","DOIUrl":"https://doi.org/10.1201/9781003066392-5","url":null,"abstract":"Secretion is an attractive production mode for proteins that require posttranslational modifications carried out in the secretory pathway. For example, amino acid chains fold properly, disulfide bonds form correctly, and glycosylation occurs accurately as the protein is secreted. In addition, recovery of secreted proteins is simplified by the fact that cells need not be broken and the product may be a major species present in a minimal synthetic culture medium. The current state of the art permits the production and secretion of proteins by heterologous cells. While future efforts will be required to improve the efficiency of secretion, current methods yield commercially interesting levels of secretion from E. coli, B. subtilis, S. cerevisiae, Aspergilli, and many mammalian cell types. Each of these systems offers certain advantages, and the choice of system depends on the specific protein to be secreted. High-value human therapeutic products such as plasminogen activators are reasonable candidates for secretion from mammalian cell lines, while industrial proteins such as calf prochymosin require a more economical host, such as baker's yeast or Aspergillus. The wide variety of posttranslational modifications observed in nature may prevent any single secretion system from dominating the field for many years to come.","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"9 1","pages":"67-94"},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49559218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Secretory Production of Homologous and Heterologous Proteins by Recombinant Streptomyces: What Has Been Accomplished?","authors":"István Molnár","doi":"10.1201/9781003067191-7","DOIUrl":"https://doi.org/10.1201/9781003067191-7","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65952517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-26DOI: 10.1201/9781003067191-23
Y. Shimada, A. Sugihara, Y. Tominaga
{"title":"Microbial Lipase: Structure and Production","authors":"Y. Shimada, A. Sugihara, Y. Tominaga","doi":"10.1201/9781003067191-23","DOIUrl":"https://doi.org/10.1201/9781003067191-23","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65952599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Uric acid is the end product of purine metabolism. It will cause physical pain and impaired function when the blood concentration exceeds a certain threshold. It is acknowledged that hyperuricemia is related to cardiovascular disease, metabolic syndrome, stroke and kidney disease. However, there is no consensus on the relationship between uric acid and different diseases and whether uric acid is an independent risk factor of these diseases. This article reviews the rela-
{"title":"Research Progress of the Relationship between Uric Acid and Disease","authors":"Lu Liu, Ting Gong","doi":"10.12677/bp.2020.103004","DOIUrl":"https://doi.org/10.12677/bp.2020.103004","url":null,"abstract":"Uric acid is the end product of purine metabolism. It will cause physical pain and impaired function when the blood concentration exceeds a certain threshold. It is acknowledged that hyperuricemia is related to cardiovascular disease, metabolic syndrome, stroke and kidney disease. However, there is no consensus on the relationship between uric acid and different diseases and whether uric acid is an independent risk factor of these diseases. This article reviews the rela-","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77709936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation between Microbiota and Oncogenesis: Research Progress","authors":"晓旭 宋","doi":"10.12677/bp.2020.103003","DOIUrl":"https://doi.org/10.12677/bp.2020.103003","url":null,"abstract":"","PeriodicalId":77040,"journal":{"name":"Bioprocess technology","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74064650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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