Eksperimental'naia onkologiia最新文献

The clonogenic activity of tumors and blood marrow cells has been studied in experiments on CBA, BALB/C and C57B1/6 mice with the Ehrlich adenocarcinoma and Lewis lung carcinosarcoma treated with adaptogenic drugs of Rhodiola Rosea extract, a synthetic analog of Rhodiola phenol derivative, methyluracil and their combinations with cyclophosphamide. The extract and derivative are shown to protect the myelopoietic tissue from the toxic action of cyclophosphamide, retaining or increasing the suppressive effect of the latter towards clonogenic tumors cells. These data can be the reason for using the extract and derivative during the antitumor chemotherapy as biological response modifiers.

Continuous administration of indomethacin (IM) in drinking water (20 micrograms/ml) causes moderate inhibition of the growth of transplantable tumors in control mice but significantly increases the antitumor effect of BCG vaccine inoculated in combination with transplanted tumor cells. Hepatoma 29, insensitive to the vaccine becomes highly sensitivity to it under IM administration.

The review presents physicochemical methods of the study of polycyclic aromatic hydrocarbons (PAH) and analysis of substances composing the exhausts of the internal combustion engines (ICE). High carcinogenic effect of the ICE exhausts due to the presence of nitro-PAHs was confirmed in the experiments with animals and the mutagenic effect was detected in the Ames test. A necessity to study carcinogenic and mutagenic effects of nitro-PAHs in a view to reveal their potential human health hazard is emphasized.

28 species of human lung cancer of the III-IV stages were investigated. The maximal growth of heterotransplants was found for them with the initial area of 1.2-1.5 mm2. The analysis of the data obtained has shown that the samples of lung adenocarcinomas grow more slowly than those of squamous cell and small-cell carcinomas and also of carcinoid.

Some physico-chemical and molecular-biological parameters of B-lymphotropic herpes virus of Macaca arctoides (HVMA) have been analyzed. The buoyant density of virions and nucleocapsids in a gradient of the percoll density is 1.10 and 1.14 g/ml, respectively, while that of DNA in CsCl gradient is 1.717 g/ml, which is typical for herpes viruses of primates. At the same time the homology of DNA HVMA with DNA EBV and DNA HVP is 30-45%. Differences in restrictions sites between DNA HVMA, DNA HVP and DNA EBV are determined. The intraspecific heterogeneity of HVMA strains has been detected according to restriction sites of DNA. The HVMA genome size is about 170 kb. The DNA HVMA is colinear to the EBV genome, which is also typical of B-lymphotropic herpes viruses of primates.

Immunohistological localization of antigens revealed by 5 MoAbs to human lung adenocarcinoma cell line was studied by indirect immunoperoxidase technique. One of the MoAbs--A2F4--showed a relative specificity to epithelial tissues having a glandular differentiation. Except approximately 50% of normal cells of lungs and those of pulmonary tumours (mostly adenocarcinomas) they reacted with some other normal (stomach, oesophagus, breast) and tumoral (gastric, mammary and colonic) tissue cells. Other four MoAbs reacted with the much larger number of cells including some nonepithelial (granulocytes and histiocytes) ones.

The dependence of the neutrophil reaction of the low-tumour strain mice CBA/CaLacY, BALB/cLacY and C57B1/6J on acute hypoxia upon the value of the initial activity (the Wilder-Leites rule is shown. This dependence is disturbed in the high-tumour strain mice AKR/JY and A/SnY. The possible presence of latent immunodeficiency in AKR/JY and A/SnY mice is discussed.

Alternative principles of regional IL-2-dependent adoptive immunocytotherapy of malignant tumours are suggested. Regional adoptive immunocytotherapy consists in the administration of the short-term IL-2 treated lymphocytes of the lymph nodes removed from the tumour which have tumour-associated suppressor activity and spleen cells after elimination of cells with the immunosuppressive activity.

The short-term effects of the carcinogenesis promoters on the adhesive force of cells are compared with the data from literature on their ability to exert a promoter action on the carcinogenesis development in the same organs during prolonged introduction after an initiator. The ability of substances to decrease the adhesive force in the tissue below the resistance threshold is established to correlate with its promoter activity. The coincidence of tissue specificity of both effects and active doses of substances is observed.

The activity of nucleoli-organizing (NOR's) regions was studied in leukemic cells of 53 children with immunologically typical ALL. The Ag-negative type of ALL was found in 9 cases of IA-ALL and Comm(+)-ALL. Dominating cells were not argentaffin. Some cytological peculiarities of Ag-negative cases have been demonstrated: lower presence of E(+)-receptor on circulating lymphoid cells, the greater number of cells with smooth surface which was revealed by the scanning electron microscopic method. The cases when the product of Ag-philic reaction was impossible to be revealed are discussed.