Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420240571
Cesar R L Amaral, Dafne Anjos, Fábio L V Bones, Antonio Carlos DE Freitas, Maithê G P Magalhães, Lucas M Moreira, Rodrigo Goldenberg-Barbosa, Anna Donato
Extremophile organisms have been largely studied in Astrobiology. Among them, two antarctic plants emerge as good candidates to become colonizers of other celestial bodies, such as Mars and the Moon. The present research aimed to evaluate survival and growing capacity of Sanionia uncinata and Colobanthus quitensis on Martian (MGS-1) and Lunar (LMS-1) regolith simulants, under terrestrial conditions. The survival responses of both species on the simulators and the original sampling site of Antarctic soil were observed during 15 days, in laboratory conditions at 'Comandante Ferraz' Station. Based on physiological parameters changes under the three soil conditions tested, our results suggest that Martian soil can be too harsh for plant growth, showing expressive decay, especially for C. quitensis. While lunar soil might provide more favorable conditions, with less observed changes, similarly to how they would in Antarctic soil from their natural habitat. This preliminary study provides resources and fosters knowledge about the possibility of these Antarctic species to survive in extraterrestrial environments, starting with soil parameters; and discusses the importance and use of Antarctic plants in astrobiology.
{"title":"May Antarctic plants grow on Martian and Lunar soil simulants under terrestrial conditions?","authors":"Cesar R L Amaral, Dafne Anjos, Fábio L V Bones, Antonio Carlos DE Freitas, Maithê G P Magalhães, Lucas M Moreira, Rodrigo Goldenberg-Barbosa, Anna Donato","doi":"10.1590/0001-3765202420240571","DOIUrl":"https://doi.org/10.1590/0001-3765202420240571","url":null,"abstract":"<p><p>Extremophile organisms have been largely studied in Astrobiology. Among them, two antarctic plants emerge as good candidates to become colonizers of other celestial bodies, such as Mars and the Moon. The present research aimed to evaluate survival and growing capacity of Sanionia uncinata and Colobanthus quitensis on Martian (MGS-1) and Lunar (LMS-1) regolith simulants, under terrestrial conditions. The survival responses of both species on the simulators and the original sampling site of Antarctic soil were observed during 15 days, in laboratory conditions at 'Comandante Ferraz' Station. Based on physiological parameters changes under the three soil conditions tested, our results suggest that Martian soil can be too harsh for plant growth, showing expressive decay, especially for C. quitensis. While lunar soil might provide more favorable conditions, with less observed changes, similarly to how they would in Antarctic soil from their natural habitat. This preliminary study provides resources and fosters knowledge about the possibility of these Antarctic species to survive in extraterrestrial environments, starting with soil parameters; and discusses the importance and use of Antarctic plants in astrobiology.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 2","pages":"e20240571"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420240584
Daniel N Krum, Carlos Ernesto G R Schaefer, Fábio S Oliveira, Roberto F M Michel, Heitor P Palma, Marcos Paulo E Tomaz, Ana Carolina P Amaral, Márcio R Francelino, Gustavo V Veloso
Salinization is one of the main pedogenetic processes occurring on the semiarid zone of the Weddel sea, like in Seymour Island, where salt-affected soils are widespread. This study aimed to investigate whether topography controls the distribution of salt-affected soils on Seymour Island, particularly with reference to the Lopez de Bertodano Formation. Chemical and physical results from 45 soil profiles were used, where morphometric variables were extracted. Descriptive statistical analysis, extraction of morphometric variables, cluster analysis and principal component analysis were carried out in this database. The separation of soils associated with the López de Bertodano Formation into two groups is explained by topographic atributes, and not by soil properties. Hence, the salts in soils have a geogenic origin, and despite differences in geological units, the uniform mineralogical composition of all Lopez de Bertodano Formation results in the widespread accumulation of salts in soils, influenced by the saline parent material and the semi-desert climate. Despite the lack of topographic control in the distribution of saline soils as a whole, geomorphology play a distinct role in the formation of secondary saline features, such as crusts, efflorescences, and columnar structures, or in salt composition of salts present in the soils.
盐碱化是发生在威德尔海半干旱区的主要成土过程之一,比如在西摩岛,受盐影响的土壤分布广泛。本研究旨在调查地形是否控制了西摩岛盐蚀土壤的分布,特别是洛佩兹德贝托达诺地层。使用了45个土壤剖面的化学和物理结果,其中提取了形态计量变量。对该数据库进行描述性统计分析、形态计量变量提取、聚类分析和主成分分析。与López de Bertodano地层相关的土壤分成两组是由地形属性而不是土壤属性来解释的。因此,土壤中的盐具有地质成因,尽管地质单位不同,但所有Lopez de Bertodano组的均匀矿物学组成导致土壤中盐的广泛积累,受含盐母质和半沙漠气候的影响。尽管在盐渍土的整体分布中缺乏地形控制,但地貌在次生盐渍特征的形成中起着明显的作用,如结壳、风化和柱状结构,或者在土壤中存在的盐的盐组成中起着重要作用。
{"title":"Salt-affected soils in the Lopez de Bertodano Formation, polar semi-desert of Seymour Island, Antarctica: topographic or lithological controls?","authors":"Daniel N Krum, Carlos Ernesto G R Schaefer, Fábio S Oliveira, Roberto F M Michel, Heitor P Palma, Marcos Paulo E Tomaz, Ana Carolina P Amaral, Márcio R Francelino, Gustavo V Veloso","doi":"10.1590/0001-3765202420240584","DOIUrl":"https://doi.org/10.1590/0001-3765202420240584","url":null,"abstract":"<p><p>Salinization is one of the main pedogenetic processes occurring on the semiarid zone of the Weddel sea, like in Seymour Island, where salt-affected soils are widespread. This study aimed to investigate whether topography controls the distribution of salt-affected soils on Seymour Island, particularly with reference to the Lopez de Bertodano Formation. Chemical and physical results from 45 soil profiles were used, where morphometric variables were extracted. Descriptive statistical analysis, extraction of morphometric variables, cluster analysis and principal component analysis were carried out in this database. The separation of soils associated with the López de Bertodano Formation into two groups is explained by topographic atributes, and not by soil properties. Hence, the salts in soils have a geogenic origin, and despite differences in geological units, the uniform mineralogical composition of all Lopez de Bertodano Formation results in the widespread accumulation of salts in soils, influenced by the saline parent material and the semi-desert climate. Despite the lack of topographic control in the distribution of saline soils as a whole, geomorphology play a distinct role in the formation of secondary saline features, such as crusts, efflorescences, and columnar structures, or in salt composition of salts present in the soils.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 2","pages":"e20240584"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420240144
Camila M Leonor, Erik Muxagata
Zooplankton plays a crucial role as the primary consumers in the Southern Ocean and its ecological processes, particularly in the Antarctic Peninsula, influenced by regional glaciological and oceanographic changes. To assess the overall composition of these communities, vertical samples were collected at various depths using a Multinet at oceanographic stations in the Antarctic Peninsula during the XXXV OPERANTAR expedition in summer 2017. Abiotic data (temperature, salinity and chlorophyll-a) were collected using a CTD and a fluoremeter. Organisms were identified to a higher level, with Chaetognatha and Euphausiacea identified to species whenever possible. Copepoda were the most abundant (73.4%), with Calanoida present in all samples and more abundant at 300m. Salpidae ranked as the second most abundant taxon (16.6%) up to 100m. Three species of Chaetognatha were identified, with Eukrohnia hamata being the most abundant, particularly at 300m. Two species of Euphausiacea were found, Euphausia superba and Thysanoesa macrura, with low abundances. Abiotic parameters showed significant relationships with the taxa. The region exhibits complex oceanography associated with zooplankton communities. The recorded data align with the zooplankton characterization of this region, uncovering a prevalence of Copepoda and surface abundant Salpidae, along with Chaetognatha (particulary E. hamata) and Euphausiacea.
{"title":"Vertical distribution of the zooplankton in the Antarctic Peninsula during the austral summer of 2017.","authors":"Camila M Leonor, Erik Muxagata","doi":"10.1590/0001-3765202420240144","DOIUrl":"https://doi.org/10.1590/0001-3765202420240144","url":null,"abstract":"<p><p>Zooplankton plays a crucial role as the primary consumers in the Southern Ocean and its ecological processes, particularly in the Antarctic Peninsula, influenced by regional glaciological and oceanographic changes. To assess the overall composition of these communities, vertical samples were collected at various depths using a Multinet at oceanographic stations in the Antarctic Peninsula during the XXXV OPERANTAR expedition in summer 2017. Abiotic data (temperature, salinity and chlorophyll-a) were collected using a CTD and a fluoremeter. Organisms were identified to a higher level, with Chaetognatha and Euphausiacea identified to species whenever possible. Copepoda were the most abundant (73.4%), with Calanoida present in all samples and more abundant at 300m. Salpidae ranked as the second most abundant taxon (16.6%) up to 100m. Three species of Chaetognatha were identified, with Eukrohnia hamata being the most abundant, particularly at 300m. Two species of Euphausiacea were found, Euphausia superba and Thysanoesa macrura, with low abundances. Abiotic parameters showed significant relationships with the taxa. The region exhibits complex oceanography associated with zooplankton communities. The recorded data align with the zooplankton characterization of this region, uncovering a prevalence of Copepoda and surface abundant Salpidae, along with Chaetognatha (particulary E. hamata) and Euphausiacea.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 2","pages":"e20240144"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420210103
Ricardo Scheffer DE Andrade Silva, Tiago Miguel Jarek, Gabriel Lucas DE Jesus, Gabriel Dias DE Oliveira, Francine Lorena Cuquel
Oyster mushroom (Pleurotus ostreatus) is the second most produced mushroom globally, but increased production is linked to new diseases. This study reports the first occurrence of Penicillium brevicompactum on P. ostreatus mushrooms. Symptoms, identified in the primordia, include atrophy, malformation, drying, and sporulation, causing up to 100% losses. The pathogen was confirmed with 99% identity via ITS region analysis and phylogenetic comparison. The research highlights P. brevicompactum's infection of mushroom primordia, emphasizing the need for pathogen identification and preventive measures to mitigate production losses.
{"title":"First report of Penicillium brevicompactum causing disease in Pleurotus ostreatus.","authors":"Ricardo Scheffer DE Andrade Silva, Tiago Miguel Jarek, Gabriel Lucas DE Jesus, Gabriel Dias DE Oliveira, Francine Lorena Cuquel","doi":"10.1590/0001-3765202420210103","DOIUrl":"https://doi.org/10.1590/0001-3765202420210103","url":null,"abstract":"<p><p>Oyster mushroom (Pleurotus ostreatus) is the second most produced mushroom globally, but increased production is linked to new diseases. This study reports the first occurrence of Penicillium brevicompactum on P. ostreatus mushrooms. Symptoms, identified in the primordia, include atrophy, malformation, drying, and sporulation, causing up to 100% losses. The pathogen was confirmed with 99% identity via ITS region analysis and phylogenetic comparison. The research highlights P. brevicompactum's infection of mushroom primordia, emphasizing the need for pathogen identification and preventive measures to mitigate production losses.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20210103"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420230368
Marcia B Alves, Katyane S Nascimento, Jéssica Mayara M Araújo, Wellyson C A Firmo, Victor Elias Mouchrek Filho, Dionney A DE Sousa, Ana Luíza M Guaraldi, Lídio G Lima Neto, Priscila S Sabbadini
The objective of this study was to investigate the chemical composition of Syzygium aromaticum essential oil (SAEO), both as its toxicity and biological activities on Corynebacterium diphtheriae. The essential oil (EO) was obtained by hydrodistillation and verified by GC-MS. The main chemical components were eugenol (48.79%), caryophyllene (44.29%) and α-humulene (4.87%). In vitro tests with human red blood cells, blood compatibility. SAEO showed high toxicity for Artemia salina and the bioassay on Tenebrio molitor larvae revealed an average survival rate of 80.00%. The antioxidant activity of SAEO was moderate (40.60%) using the phosphomolybdenum method. SAEO inhibited the growth of all C. diphtheriae strains tested at minimum inhibitory concentrations of 62.5-1000 µg mL-1 (strong to moderate). The minimum bactericidal concentration of SAEO was observed in two strains. Microorganisms cultivated in the presence of subinhibitory concentrations (subMIC) do not show morphological changes. The subMIC of SAEO inhibited in some strains, but in most cases, increased biofilm formation. In conclusion, the results of this study found that SAEO has an inhibitory effect against C. diphtheriae, representing an alternative antimicrobial therapy, although more in vivo biological studies, alone or in combination with occasional antimicrobials, should be carried out to better evaluate its effects.
{"title":"Gas Chromatography-mass Spectrometry analysis, Biotoxicity and Antibiofilm Activities of Syzygium aromaticum against Corynebacterium diphtheriae.","authors":"Marcia B Alves, Katyane S Nascimento, Jéssica Mayara M Araújo, Wellyson C A Firmo, Victor Elias Mouchrek Filho, Dionney A DE Sousa, Ana Luíza M Guaraldi, Lídio G Lima Neto, Priscila S Sabbadini","doi":"10.1590/0001-3765202420230368","DOIUrl":"https://doi.org/10.1590/0001-3765202420230368","url":null,"abstract":"<p><p>The objective of this study was to investigate the chemical composition of Syzygium aromaticum essential oil (SAEO), both as its toxicity and biological activities on Corynebacterium diphtheriae. The essential oil (EO) was obtained by hydrodistillation and verified by GC-MS. The main chemical components were eugenol (48.79%), caryophyllene (44.29%) and α-humulene (4.87%). In vitro tests with human red blood cells, blood compatibility. SAEO showed high toxicity for Artemia salina and the bioassay on Tenebrio molitor larvae revealed an average survival rate of 80.00%. The antioxidant activity of SAEO was moderate (40.60%) using the phosphomolybdenum method. SAEO inhibited the growth of all C. diphtheriae strains tested at minimum inhibitory concentrations of 62.5-1000 µg mL-1 (strong to moderate). The minimum bactericidal concentration of SAEO was observed in two strains. Microorganisms cultivated in the presence of subinhibitory concentrations (subMIC) do not show morphological changes. The subMIC of SAEO inhibited in some strains, but in most cases, increased biofilm formation. In conclusion, the results of this study found that SAEO has an inhibitory effect against C. diphtheriae, representing an alternative antimicrobial therapy, although more in vivo biological studies, alone or in combination with occasional antimicrobials, should be carried out to better evaluate its effects.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20230368"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420231369
Ricardo A Pereira, Albimara Hey, Aline B DE Jesus, Aline T Marin, Fernanda Fiorini, Renata Corassa, Ana Karolina S Goes, Mayara Grolli, Anayana Z Dangui, Jéssica Wouk, Anaclara Prasniewski, Tatiane Luiza C Oldoni, Giuseppina Paola Parpinello, Urszula Tylewicz, Emerson Carraro, Carlos Ricardo M Malfatti
This article reports the development of a beer made with Baccharis dracunculifolia and its application in an experimental model of diabetes. Initially, the production of the beverage was standardized in order to incorporate the plant extract properly. Next, the beer was analyzed by the UHPLC-MS to identify the substances present. Among others, caffeic acid (5.85 mg / L), m-coumaric acid (5.16 mg / L), pinocembrin (2.99 mg / L), chrysin (10.86 mg / L), myricetin (1.73 mg / L) and spathulenol (9.30 mg / L) were found. Animal tests indicate improvement in biochemical and histological parameters of STZ-induced Wistar rats that ingested the beer made with the plant. The antidiabetic potential of this beverage was observed in the different tests that evaluated insulin resistance and the decrease of the clinical manifestations of diabetes in animals. The use of the drink as an adjunct in clinical treatments for DM2 may be useful, especially in suppressing the oxidative damage caused by the disease.
{"title":"Biochemical and histological effects of the subchronic treatment with a beer containing Baccharis dracunculifolia in an experimental model of diabetes.","authors":"Ricardo A Pereira, Albimara Hey, Aline B DE Jesus, Aline T Marin, Fernanda Fiorini, Renata Corassa, Ana Karolina S Goes, Mayara Grolli, Anayana Z Dangui, Jéssica Wouk, Anaclara Prasniewski, Tatiane Luiza C Oldoni, Giuseppina Paola Parpinello, Urszula Tylewicz, Emerson Carraro, Carlos Ricardo M Malfatti","doi":"10.1590/0001-3765202420231369","DOIUrl":"https://doi.org/10.1590/0001-3765202420231369","url":null,"abstract":"<p><p>This article reports the development of a beer made with Baccharis dracunculifolia and its application in an experimental model of diabetes. Initially, the production of the beverage was standardized in order to incorporate the plant extract properly. Next, the beer was analyzed by the UHPLC-MS to identify the substances present. Among others, caffeic acid (5.85 mg / L), m-coumaric acid (5.16 mg / L), pinocembrin (2.99 mg / L), chrysin (10.86 mg / L), myricetin (1.73 mg / L) and spathulenol (9.30 mg / L) were found. Animal tests indicate improvement in biochemical and histological parameters of STZ-induced Wistar rats that ingested the beer made with the plant. The antidiabetic potential of this beverage was observed in the different tests that evaluated insulin resistance and the decrease of the clinical manifestations of diabetes in animals. The use of the drink as an adjunct in clinical treatments for DM2 may be useful, especially in suppressing the oxidative damage caused by the disease.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20231369"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420230677
Kyria S Nascimento, Vinicius J S Osterne, Messias V Oliveira, Jorge L C Domingos, Wandemberg P Ferreira, Els J M VAN Damme, Benildo S Cavada, Vanir R Pinto-Junior
Understanding lectin-carbohydrate interactions at the structural and molecular levels is crucial to the field of lectins, as the diverse roles and biological activities exhibited by these proteins are fundamentally linked to their specific binding to target glycoconjugates. This study aimed to apply molecular dynamics to analyze the structure and binding properties of Parkia lectins. 3D structures of Parkia platycephala and P. biglobosa lectins, both unliganded and in complex with D-mannose, were used as inputs for simulations. The trajectories data enabled the study of stability, carbohydrate-binding interactions, and intermonomeric contacts for both proteins. The results revealed stable binding of D-mannose within the lectin domains and their binding mode at each of the three domains, displaying consistent binding motifs across the sites, with slight variations between the lectins and other Jacalin-related lectins. Despite these variations, the binding energies of the lectins with the ligand, as estimated using MM/GBSA, demonstrated favorable interactions in all cases. The dimeric interfaces of both lectins could be identified, and the main contacts have been mapped. These findings enhance our understanding of lectin-carbohydrate interactions and provide insights into the structural properties of Parkia lectins for potential biological and therapeutic applications.
{"title":"Lectin-carbohydrate analysis by molecular dynamics: Parkia lectins case study.","authors":"Kyria S Nascimento, Vinicius J S Osterne, Messias V Oliveira, Jorge L C Domingos, Wandemberg P Ferreira, Els J M VAN Damme, Benildo S Cavada, Vanir R Pinto-Junior","doi":"10.1590/0001-3765202420230677","DOIUrl":"https://doi.org/10.1590/0001-3765202420230677","url":null,"abstract":"<p><p>Understanding lectin-carbohydrate interactions at the structural and molecular levels is crucial to the field of lectins, as the diverse roles and biological activities exhibited by these proteins are fundamentally linked to their specific binding to target glycoconjugates. This study aimed to apply molecular dynamics to analyze the structure and binding properties of Parkia lectins. 3D structures of Parkia platycephala and P. biglobosa lectins, both unliganded and in complex with D-mannose, were used as inputs for simulations. The trajectories data enabled the study of stability, carbohydrate-binding interactions, and intermonomeric contacts for both proteins. The results revealed stable binding of D-mannose within the lectin domains and their binding mode at each of the three domains, displaying consistent binding motifs across the sites, with slight variations between the lectins and other Jacalin-related lectins. Despite these variations, the binding energies of the lectins with the ligand, as estimated using MM/GBSA, demonstrated favorable interactions in all cases. The dimeric interfaces of both lectins could be identified, and the main contacts have been mapped. These findings enhance our understanding of lectin-carbohydrate interactions and provide insights into the structural properties of Parkia lectins for potential biological and therapeutic applications.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20230677"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420240578
Rosemary Vieira, Priscila Cardoso, Kátia K DA Rosa, Carina Petsch, Juan Manuel Lirio
Some Antarctic ice-free areas have been affected by changes in lacustrine zones and permafrost thawing due to rising air temperatures over the last 60 years Temperature time-series were analyzed to understand the processes leading to the changes of Boeckella and Buenos Aires lakes, north Antarctic Peninsula. Statistical calculations were applied to average, maximum, and minimum temperatures, as well as to indexes such as Positive Degrees Day, Freezing/Thaw Day, and days with temperatures ≥ 0 °C and ≥ 10 °C. Changes in the region over recent decades were observed in mean and maximum temperatures and an increase in minimum temperature since the mid-1990s. In addition, the Normalized Difference Water Index was extracted from Landsat images from 2003, 2016, 2020, and 2021. These images were used to calculate changes in the areas of Boeckella and Buenos Aires lakes. These data revealed an 87% decrease in the area (m²) of Boeckella Lake and a 60% increase in Buenos Aires Lake´s area (m²). Concern has been raised about the modifying lake areas in the Antarctic Peninsula, where the collapse of Boeckella Lake could be the first case recorded in ice-free areas of Antarctica continent.
{"title":"Changes and collapse in lacustrine system in Antarctic Peninsula ice-free area: Boeckella and Buenos Aires lakes.","authors":"Rosemary Vieira, Priscila Cardoso, Kátia K DA Rosa, Carina Petsch, Juan Manuel Lirio","doi":"10.1590/0001-3765202420240578","DOIUrl":"https://doi.org/10.1590/0001-3765202420240578","url":null,"abstract":"<p><p>Some Antarctic ice-free areas have been affected by changes in lacustrine zones and permafrost thawing due to rising air temperatures over the last 60 years Temperature time-series were analyzed to understand the processes leading to the changes of Boeckella and Buenos Aires lakes, north Antarctic Peninsula. Statistical calculations were applied to average, maximum, and minimum temperatures, as well as to indexes such as Positive Degrees Day, Freezing/Thaw Day, and days with temperatures ≥ 0 °C and ≥ 10 °C. Changes in the region over recent decades were observed in mean and maximum temperatures and an increase in minimum temperature since the mid-1990s. In addition, the Normalized Difference Water Index was extracted from Landsat images from 2003, 2016, 2020, and 2021. These images were used to calculate changes in the areas of Boeckella and Buenos Aires lakes. These data revealed an 87% decrease in the area (m²) of Boeckella Lake and a 60% increase in Buenos Aires Lake´s area (m²). Concern has been raised about the modifying lake areas in the Antarctic Peninsula, where the collapse of Boeckella Lake could be the first case recorded in ice-free areas of Antarctica continent.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 2","pages":"e20240578"},"PeriodicalIF":1.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-09eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420240555
Danielle D Soffiatti, Kátia K Rosa, Júlia L Lorenz, Filipe Lindau, Carina Petsch, Francisco E Aquino, Jefferson C Simões
Glaciers are sensitive to environmental climatic conditions and show their variability over time. This study investigates the environmental characteristics and variation in glacial cover of the Greenwich, Livingston, Robert and Snow islands, Antarctica, between 1956 and 2023. The glacier extension mapping was based on visual interpretation of the Landsat 4, 7 and 8 optical images and normalised difference indexes combination. The REMA 8 digital elevation model was applied to the islands' geomorphometric analysis. Results were compared to climatic series and environmental characteristics. The glacial coverage decreased by 16.9% from 1956 to 2023, equivalent to 209.95 ± 0.113 km². Snow Island, with shallow bathymetry near glacial margins and land-terminating ice-fronts, exhibited the smallest retreat rate values. The glacial coverage of Livingston Island decreased significantly (18%, equivalent to 153.2 ± 0.113 km²) from 1956-2021, representing the highest values in the study. The changes in glacial coverage of the Robert and Greenwich islands were more substantial in the 1989-2023 period than in 1956-1989. Sectors with marine-terminating glaciers, associated with high bathymetric amplitudes, exhibited more significant variations compared to other sectors, contributing to the evolution of fjord-type bays. Glacial coverage has been lost in recent decades due to regional atmospheric and oceanic warming.
{"title":"Retreat of Greenwich, Livingston, Robert and Snow Islands glaciers, Antarctica, between 1956 and 2023.","authors":"Danielle D Soffiatti, Kátia K Rosa, Júlia L Lorenz, Filipe Lindau, Carina Petsch, Francisco E Aquino, Jefferson C Simões","doi":"10.1590/0001-3765202420240555","DOIUrl":"https://doi.org/10.1590/0001-3765202420240555","url":null,"abstract":"<p><p>Glaciers are sensitive to environmental climatic conditions and show their variability over time. This study investigates the environmental characteristics and variation in glacial cover of the Greenwich, Livingston, Robert and Snow islands, Antarctica, between 1956 and 2023. The glacier extension mapping was based on visual interpretation of the Landsat 4, 7 and 8 optical images and normalised difference indexes combination. The REMA 8 digital elevation model was applied to the islands' geomorphometric analysis. Results were compared to climatic series and environmental characteristics. The glacial coverage decreased by 16.9% from 1956 to 2023, equivalent to 209.95 ± 0.113 km². Snow Island, with shallow bathymetry near glacial margins and land-terminating ice-fronts, exhibited the smallest retreat rate values. The glacial coverage of Livingston Island decreased significantly (18%, equivalent to 153.2 ± 0.113 km²) from 1956-2021, representing the highest values in the study. The changes in glacial coverage of the Robert and Greenwich islands were more substantial in the 1989-2023 period than in 1956-1989. Sectors with marine-terminating glaciers, associated with high bathymetric amplitudes, exhibited more significant variations compared to other sectors, contributing to the evolution of fjord-type bays. Glacial coverage has been lost in recent decades due to regional atmospheric and oceanic warming.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 2","pages":"e20240555"},"PeriodicalIF":1.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-09eCollection Date: 2024-01-01DOI: 10.1590/0001-3765202420240880
Adriano Emanuel Machado, Peterson Rezer, Gianni Mancini, Alexandra Latini, Eduardo Luiz G Moreira
Understanding the temporal dynamics of high-fat diets (HFD) effects on behavior and metabolism is crucial for comprehending their negative impact on organisms. This study investigated the short-term effects (15, 25, and 35 days) of HFD in Swiss mice. Our findings revealed distinct behavioral and metabolic changes throughout the treatment. After 15 days of HFD, mice exhibited impaired exploratory habituation and significant increases in visceral adipose mass, fasting glucose levels, and glucose intolerance. Extending the diet to 25 days intensified the metabolic effects, resulting in compromised acquisition of recognition memory, increased body mass gain, and elevated plasma total cholesterol and triglyceride levels. After 35 days of HFD, these effects were further intensified and accompanied by anxiogenic-like responses in the open field test. Additionally, we observed a positive correlation between metabolic changes and behavioral impairments alongside prefrontal cortex mitochondrial dysfunction. In conclusion, our study reveals the temporal dynamics of behavioral and metabolic changes induced by short-term HFD in Swiss mice, highlighting the relationship between metabolic dysfunction and behavioral impairments. These findings pave the way for future research to unravel the underlying mechanisms and develop strategies to counteract the detrimental effects of HFD on behavior and metabolism.
{"title":"Short-term high-fat diet alters behavior, peripheral metabolism, and brain mitochondrial function in Swiss mice.","authors":"Adriano Emanuel Machado, Peterson Rezer, Gianni Mancini, Alexandra Latini, Eduardo Luiz G Moreira","doi":"10.1590/0001-3765202420240880","DOIUrl":"10.1590/0001-3765202420240880","url":null,"abstract":"<p><p>Understanding the temporal dynamics of high-fat diets (HFD) effects on behavior and metabolism is crucial for comprehending their negative impact on organisms. This study investigated the short-term effects (15, 25, and 35 days) of HFD in Swiss mice. Our findings revealed distinct behavioral and metabolic changes throughout the treatment. After 15 days of HFD, mice exhibited impaired exploratory habituation and significant increases in visceral adipose mass, fasting glucose levels, and glucose intolerance. Extending the diet to 25 days intensified the metabolic effects, resulting in compromised acquisition of recognition memory, increased body mass gain, and elevated plasma total cholesterol and triglyceride levels. After 35 days of HFD, these effects were further intensified and accompanied by anxiogenic-like responses in the open field test. Additionally, we observed a positive correlation between metabolic changes and behavioral impairments alongside prefrontal cortex mitochondrial dysfunction. In conclusion, our study reveals the temporal dynamics of behavioral and metabolic changes induced by short-term HFD in Swiss mice, highlighting the relationship between metabolic dysfunction and behavioral impairments. These findings pave the way for future research to unravel the underlying mechanisms and develop strategies to counteract the detrimental effects of HFD on behavior and metabolism.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 3","pages":"e20240880"},"PeriodicalIF":1.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}