C. Winstead, F. Mazzotti, J. Mierzwa, G. P. Miller
We report the first experimental results using electrothermal atomization-cavity ringdown spectroscopy for trace elemental analysis. Lead aqueous standard solutions were analyzed using graphite furnace-cavity ringdown spectroscopy and a preliminary detection limit of approximately 1 pg was obtained for peak height absorption measurements. No chemical matrix modifiers were used. These preliminary results together with an estimate of theoretical detection limits suggest that the coupling of ETA and CRDS has the potential to become a valuable new technique in analytical atomic spectrometry.
{"title":"Preliminary results for electrothermal atomization-cavity ringdown spectroscopy (ETA-CRDS)","authors":"C. Winstead, F. Mazzotti, J. Mierzwa, G. P. Miller","doi":"10.1039/A903563J","DOIUrl":"https://doi.org/10.1039/A903563J","url":null,"abstract":"We report the first experimental results using electrothermal atomization-cavity ringdown spectroscopy for trace elemental analysis. Lead aqueous standard solutions were analyzed using graphite furnace-cavity ringdown spectroscopy and a preliminary detection limit of approximately 1 pg was obtained for peak height absorption measurements. No chemical matrix modifiers were used. These preliminary results together with an estimate of theoretical detection limits suggest that the coupling of ETA and CRDS has the potential to become a valuable new technique in analytical atomic spectrometry.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"96 1","pages":"277-279"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80075602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Casiot, V. Vacchina, H. Chassaigne, J. Szpunar, M. Potin-Gautier, R. Łobiński
An approach to the identification of unknown signals in selenium speciation analysis of yeast by reversed-phase chromatography with ICP-MS detection is described. The analytical strategy was based on: (i), heart-cutting of a Se-containing fraction in the reversed-phase chromatographic eluate followed by its lyophilization; (ii), pneumatically-assisted electrospray (ESI) MS and ESI tandem MS of the lyophilizate; and (iii) confirmation of the fragmentation pattern obtained using the sulfur analogue of the seleno compound that was expected to have been identified. The approach developed allowed the identification of Se–adenosylhomocysteine as the major selenium species in an extract of a selenized yeast sample.
{"title":"An approach to the identification of selenium species in yeast extracts using pneumatically-assisted electrospray tandem mass spectrometry","authors":"C. Casiot, V. Vacchina, H. Chassaigne, J. Szpunar, M. Potin-Gautier, R. Łobiński","doi":"10.1039/A900319C","DOIUrl":"https://doi.org/10.1039/A900319C","url":null,"abstract":"An approach to the identification of unknown signals in selenium speciation analysis of yeast by reversed-phase chromatography with ICP-MS detection is described. The analytical strategy was based on: (i), heart-cutting of a Se-containing fraction in the reversed-phase chromatographic eluate followed by its lyophilization; (ii), pneumatically-assisted electrospray (ESI) MS and ESI tandem MS of the lyophilizate; and (iii) confirmation of the fragmentation pattern obtained using the sulfur analogue of the seleno compound that was expected to have been identified. The approach developed allowed the identification of Se–adenosylhomocysteine as the major selenium species in an extract of a selenized yeast sample.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"78 1","pages":"77-80"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84060520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of functional and cross-linking monomers and the amount of template on the performance of molecularly imprinted polymers in binding assays","authors":"E. Yilmaz, K. Mosbach, K. Haupt","doi":"10.1039/A901339C","DOIUrl":"https://doi.org/10.1039/A901339C","url":null,"abstract":"","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"60 1","pages":"167-170"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84638272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A gas chromatographic system has been developed for the direct analysis of atmospheric formaldehyde and other oxygenated hydrocarbons. This method utilises the trapping of analytes in a loop cooled with liquid nitrogen, separation by gas chromatography and then subsequent detection using a pulsed discharge helium ionisation detector (pdHID). The detection limit of this instrument is estimated to be 32 parts per trillion by volume (pptv) for 0.2 l of gas sampled at a flow rate of 30 ml min–1 (S/N at 4:1). A number of other compounds have been monitored simultaneously using this method, including higher molecular weight aldehydes and acetone. Continuous measurement of formaldehyde can be performed with a time resolution of 15 min, with longer analysis times required for inclusion of other species. Calibrations were performed using a permeation tube instrument for both formaldehyde and acetone. A linear response has been observed for formaldehyde sample volumes of 60–200 ml of a 272 ppbv sample (20–68 ng). For acetone a linear response was observed from 175–360 ml of a 30 ppbv standard (12–26 ng). A flame ionisation detector (FID) was also utilised during system development to confirm separation of formaldehyde from atmospheric hydrocarbons.
{"title":"Direct measurement of atmospheric formaldehyde using gas chromatography-pulsed discharge ionisation detection","authors":"M. Hunter, K. Bartle, P. Seakins, A. Lewis","doi":"10.1039/A809762C","DOIUrl":"https://doi.org/10.1039/A809762C","url":null,"abstract":"A gas chromatographic system has been developed for the direct analysis of atmospheric formaldehyde and other oxygenated hydrocarbons. This method utilises the trapping of analytes in a loop cooled with liquid nitrogen, separation by gas chromatography and then subsequent detection using a pulsed discharge helium ionisation detector (pdHID). The detection limit of this instrument is estimated to be 32 parts per trillion by volume (pptv) for 0.2 l of gas sampled at a flow rate of 30 ml min–1 (S/N at 4:1). A number of other compounds have been monitored simultaneously using this method, including higher molecular weight aldehydes and acetone. Continuous measurement of formaldehyde can be performed with a time resolution of 15 min, with longer analysis times required for inclusion of other species. Calibrations were performed using a permeation tube instrument for both formaldehyde and acetone. A linear response has been observed for formaldehyde sample volumes of 60–200 ml of a 272 ppbv sample (20–68 ng). For acetone a linear response was observed from 175–360 ml of a 30 ppbv standard (12–26 ng). A flame ionisation detector (FID) was also utilised during system development to confirm separation of formaldehyde from atmospheric hydrocarbons.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"94 1","pages":"101-104"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86743579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A new methodology, sandwich-type monitoring assay system using an estrogen response element (ERE)-immobilized piezoelectric biosensor in combination with flow injection technique, has been developed for analysis of estrogen (E). The principle of the assay is that the estrogen receptor (ER) captures estrogen and then the complex is bound with ERE immobilized on the sensor. It was confirmed that initial binding of the ERE with ER is significantly accelerated by the formation of an E–ER complex. This kinetic difference was monitored by real-time measurement of the resonance frequency of the ERE piezoelectric biosensor and applied to detect 17β-estradiol. The calibration graph was linear in the range 10–100 nmol l–1 with a detection limit of 7.8 nmol l–1, an RSD of 5.9% for 50 nmol l–1 (6 replicates), and one run time of 4 min. Gradient flow injection on-line connection to an array of ERE piezoelectric biosensors is in progress for the simultaneous determination of endogenous and synthetic estrogens in drinking water and urine. In comparison with present chromatographic methods coupled with MS, the proposed technique is simple, flexible and cheap and has great potential in applications such as field and on-site monitoring and screen testing of endocrine disruptors.
{"title":"A new sandwich-type assay of estrogen using piezoelectric biosensor immobilized with estrogen response element","authors":"Mo Zhihong, Long Xiaohui, F. Weiling","doi":"10.1039/A902872B","DOIUrl":"https://doi.org/10.1039/A902872B","url":null,"abstract":"A new methodology, sandwich-type monitoring assay system using an estrogen response element (ERE)-immobilized piezoelectric biosensor in combination with flow injection technique, has been developed for analysis of estrogen (E). The principle of the assay is that the estrogen receptor (ER) captures estrogen and then the complex is bound with ERE immobilized on the sensor. It was confirmed that initial binding of the ERE with ER is significantly accelerated by the formation of an E–ER complex. This kinetic difference was monitored by real-time measurement of the resonance frequency of the ERE piezoelectric biosensor and applied to detect 17β-estradiol. The calibration graph was linear in the range 10–100 nmol l–1 with a detection limit of 7.8 nmol l–1, an RSD of 5.9% for 50 nmol l–1 (6 replicates), and one run time of 4 min. Gradient flow injection on-line connection to an array of ERE piezoelectric biosensors is in progress for the simultaneous determination of endogenous and synthetic estrogens in drinking water and urine. In comparison with present chromatographic methods coupled with MS, the proposed technique is simple, flexible and cheap and has great potential in applications such as field and on-site monitoring and screen testing of endocrine disruptors.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"2 1","pages":"281-283"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84101921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Vanhaecke, Marieke Verstraete, L. Moens, R. Dams
Electrothermal vaporization inductively coupled plasma mass spectrometry (ETV-ICPMS) was used for the determination of the Pd content in a solid aliphatic polyketone. The presence of Pd in this material has to be attributed to the use of a Pd-containing homogeneous catalyst for its production. By application of a multi-step heating programme, the organic matrix could be selectively and completely removed prior to the vaporization of the analyte element. The accuracy attainable when using (i) external calibration and (ii) single standard addition using an aqueous standard solution was evaluated by comparison of the results obtained with a reference value obtained by means of neutron activation analysis (NAA). On the basis of its similar furnace behaviour, Ir was shown to be perfectly suited to be used as an internal standard. Also the argon dimer signal (Ar2+) shows some potential to be used as an internal standard. When using Ir or Ar2+ as an internal standard and single standard addition for calibration, the ETV-ICPMS result (∼5 µg g–1) agreed within 10% with the NAA result on each occasion (deviation between average ETV-ICPMS result and reference value ∼2%). The absolute limit of detection (3s-criterion) was observed to be ∼1 pg, corresponding to a relative value of ∼1 ng g–1, taking 1 mg as a typical sample mass. However, when using Ir as an internal standard, it was established that the detection limit deteriorated to ∼20 pg, due to the presence of a measurable amount of Pd in the Ir standard solution.
{"title":"Determination of the palladium content in a solid plastic material by electrothermal vaporization ICP-mass spectrometry (ETV-ICPMS)","authors":"F. Vanhaecke, Marieke Verstraete, L. Moens, R. Dams","doi":"10.1039/A809318K","DOIUrl":"https://doi.org/10.1039/A809318K","url":null,"abstract":"Electrothermal vaporization inductively coupled plasma mass spectrometry (ETV-ICPMS) was used for the determination of the Pd content in a solid aliphatic polyketone. The presence of Pd in this material has to be attributed to the use of a Pd-containing homogeneous catalyst for its production. By application of a multi-step heating programme, the organic matrix could be selectively and completely removed prior to the vaporization of the analyte element. The accuracy attainable when using (i) external calibration and (ii) single standard addition using an aqueous standard solution was evaluated by comparison of the results obtained with a reference value obtained by means of neutron activation analysis (NAA). On the basis of its similar furnace behaviour, Ir was shown to be perfectly suited to be used as an internal standard. Also the argon dimer signal (Ar2+) shows some potential to be used as an internal standard. When using Ir or Ar2+ as an internal standard and single standard addition for calibration, the ETV-ICPMS result (∼5 µg g–1) agreed within 10% with the NAA result on each occasion (deviation between average ETV-ICPMS result and reference value ∼2%). The absolute limit of detection (3s-criterion) was observed to be ∼1 pg, corresponding to a relative value of ∼1 ng g–1, taking 1 mg as a typical sample mass. However, when using Ir as an internal standard, it was established that the detection limit deteriorated to ∼20 pg, due to the presence of a measurable amount of Pd in the Ir standard solution.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"39 1","pages":"89-92"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84294838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Ci, Chun-yang Zhang, Jun Feng, Ai-dong Lang, Chun‐hui Huang, Zhengfan Jiang
A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.
{"title":"A photoelectric method for analyzing cell-free apoptosis induced by dATP","authors":"Y. Ci, Chun-yang Zhang, Jun Feng, Ai-dong Lang, Chun‐hui Huang, Zhengfan Jiang","doi":"10.1039/A900317G","DOIUrl":"https://doi.org/10.1039/A900317G","url":null,"abstract":"A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"48 1","pages":"143-145"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80384314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A method for measuring the release of lanthanum from some ceramic dental materials was required using air–acetylene atomic absorption spectrophotometry (AA-AAS), and an analytical procedure was devised based on the release of calcium in the presence of phosphate by lanthanum addition. The extent of phosphate interference in the determination of calcium by AA-AAS was assessed, and it was shown that the absorbance of a 10 ppm Ca standard was reduced by 45% in the presence of 20 ppm or more of phosphate (as PO4). Analysis of standards containing 10 ppm Ca, 20 ppm PO4, and lanthanum added at concentrations up to 100 ppm showed rapid increase of calcium absorbances from 10 to 40 ppm La, after which absorbance increased slowly to a constant value at 90 ppm La. This corresponded to the value of a 10 ppm Ca standard solution containing no phosphate. Closer examination of solutions containing 10–40 ppm La revealed a quantitative relationship between lanthanum levels and calcium absorbances which deviated slightly from Beer’s law. Consequent analysis of solutions containing various amounts of lanthanum in the presence of 10 ppm Ca and 20 ppm PO4 followed by repeated analysis of standards demonstrated good precision and reproducibility. The relative standard deviation for repeated standard analyses was 4.2%, and the detection limit was 0.6 ppm La representing an increase of sensitivity of approximately 100 times over lanthanum determinations using nitrous oxide–acetylene techniques. The method indicates that similar procedures may be used to estimate elements which exercise similar release effects.
{"title":"The estimation of lanthanum by air-acetylene atomic absorption spectrophotometry using an indirect procedure","authors":"S. J. Wilson, P. Marquis","doi":"10.1039/A900331B","DOIUrl":"https://doi.org/10.1039/A900331B","url":null,"abstract":"A method for measuring the release of lanthanum from some ceramic dental materials was required using air–acetylene atomic absorption spectrophotometry (AA-AAS), and an analytical procedure was devised based on the release of calcium in the presence of phosphate by lanthanum addition. The extent of phosphate interference in the determination of calcium by AA-AAS was assessed, and it was shown that the absorbance of a 10 ppm Ca standard was reduced by 45% in the presence of 20 ppm or more of phosphate (as PO4). Analysis of standards containing 10 ppm Ca, 20 ppm PO4, and lanthanum added at concentrations up to 100 ppm showed rapid increase of calcium absorbances from 10 to 40 ppm La, after which absorbance increased slowly to a constant value at 90 ppm La. This corresponded to the value of a 10 ppm Ca standard solution containing no phosphate. Closer examination of solutions containing 10–40 ppm La revealed a quantitative relationship between lanthanum levels and calcium absorbances which deviated slightly from Beer’s law. Consequent analysis of solutions containing various amounts of lanthanum in the presence of 10 ppm Ca and 20 ppm PO4 followed by repeated analysis of standards demonstrated good precision and reproducibility. The relative standard deviation for repeated standard analyses was 4.2%, and the detection limit was 0.6 ppm La representing an increase of sensitivity of approximately 100 times over lanthanum determinations using nitrous oxide–acetylene techniques. The method indicates that similar procedures may be used to estimate elements which exercise similar release effects.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"163 1","pages":"31-33"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78068773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. M. Seifar, R. J. Dijkstra, U. Brinkman, C. Gooijer
At-line coupling of surface-enhanced resonance Raman spectroscopy (SERRS) and reversed-phase ion-pair chromatography with an excitation wavelength of 514.5 nm was applied for the isocratic separation and identification of four representative basic dyes (basic fuchsin, nile blue, crystal violet and ethyl violet). The effluent from a chromatographic column was deposited on a thin layer chromatography (TLC) plate by means of a spray-jet interface and analysed by Raman spectroscopy. The presence of non-volatile ion-pair reagents (1-heptanesulfonic acid sodium salt, sodium perchlorate) did not interfere with the deposition process and also not with the SERRS experiments. In fact higher SERRS intensities were observed in the presence of the ion-pair reagents. Reliable spectra could be recorded down to 90 pg of dyes deposited on the TLC plate.
{"title":"At-line coupling of surface-enhanced resonance Raman spectroscopy and reversed-phase ion-pair chromatography","authors":"R. M. Seifar, R. J. Dijkstra, U. Brinkman, C. Gooijer","doi":"10.1039/A903254A","DOIUrl":"https://doi.org/10.1039/A903254A","url":null,"abstract":"At-line coupling of surface-enhanced resonance Raman spectroscopy (SERRS) and reversed-phase ion-pair chromatography with an excitation wavelength of 514.5 nm was applied for the isocratic separation and identification of four representative basic dyes (basic fuchsin, nile blue, crystal violet and ethyl violet). The effluent from a chromatographic column was deposited on a thin layer chromatography (TLC) plate by means of a spray-jet interface and analysed by Raman spectroscopy. The presence of non-volatile ion-pair reagents (1-heptanesulfonic acid sodium salt, sodium perchlorate) did not interfere with the deposition process and also not with the SERRS experiments. In fact higher SERRS intensities were observed in the presence of the ion-pair reagents. Reliable spectra could be recorded down to 90 pg of dyes deposited on the TLC plate.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"89 1","pages":"273-276"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80386732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A very simple, rapid and highly sensitive fluorimetric method for the determination of enoxacin (ENX) is described. The method is based on the formation of Tb(III)–ENX complex in the aqueous solution, which fluoresces intensely with an emission maximum at 545 nm (Tb3+∶5D4 → 7F5) when excited at 335 nm. Optimum conditions for the determination were investigated. A linear relationship was obtained between the fluorescence intensity and ENX concentration in the range 8.0 × 10–9–6.0 × 10–6 M. The limit of detection was 5.9 × 10–10 M. Analytical recoveries from untreated urine samples spiked with ENX were in the range 95–102.5%.
{"title":"Study on fluorescence of the Tb(III)–enoxacin system and the determination of enoxacin","authors":"Y. Fangtian, J. Linpei, Z. Huichun","doi":"10.1039/A902452B","DOIUrl":"https://doi.org/10.1039/A902452B","url":null,"abstract":"A very simple, rapid and highly sensitive fluorimetric method for the determination of enoxacin (ENX) is described. The method is based on the formation of Tb(III)–ENX complex in the aqueous solution, which fluoresces intensely with an emission maximum at 545 nm (Tb3+∶5D4 → 7F5) when excited at 335 nm. Optimum conditions for the determination were investigated. A linear relationship was obtained between the fluorescence intensity and ENX concentration in the range 8.0 × 10–9–6.0 × 10–6 M. The limit of detection was 5.9 × 10–10 M. Analytical recoveries from untreated urine samples spiked with ENX were in the range 95–102.5%.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"1 1","pages":"231-233"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90833644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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