Animal Bioscience最新文献_第9页

Weighted gene co-expression network analysis identifies important modules and hub genes involved in the regulation of breast muscle yield in broilers. 加权基因共表达网络分析确定了参与肉鸡胸肌产量调控的重要模块和枢纽基因。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-25 DOI: 10.5713/ab.23.0548

Xing Guo, Hao Wang, Meng Liu, Jinmei Xu, Yanan Liu, Hong Zhang, Xin-Xin He, Jiang-Xian Wang, Wei Wei, Da-Long Ren, Runshen Jiang

ObjectiveIncreasing breast meat production is one of the primary goals of the broiler industry. Over the past few decades, tremendous progress has been made in genetic selection and the identification of candidate genes for improving the breast muscle mass. However, the molecular network contributing to muscle production traits in chickens still needs to be further illuminated.MethodsA total of 150 1-day-old male 817 broilers were reared in a floor litter system. At the market age of 50 d, eighteen healthy 817 broilers were slaughtered and the left pectoralis major muscle sample from each bird was collected for RNA-seq sequencing. The birds were then plucked and eviscerated and the whole breast muscle was removed and weighed. Breast muscle yield was calculated as the ratio of the breast muscle weight to the eviscerated weight. To identify the co-expression networks and hub genes contributing to breast muscle yield in chickens, we performed weighted gene co-expression network analysis (WGCNA) based on the 18 transcriptome datasets of pectoralis major muscle from eighteen 817 broilers.ResultsThe WGCNA analysis classified all co-expressed genes in the pectoral muscle of 817 broilers into 44 modules. Among these modules, the turquoise and skyblue3 modules were found to be most significantly positively (r=0.78, p=1e-04) and negatively (r=-0.57, p=0.01) associated with breast meat yield, respectively. Further analysis identified several hub genes (e.g., DLX3, SH3RF2, TPM1, CAV3, MYF6, and CFL2) that involved in muscle structure and muscle development were identified as potential regulators of breast meat production.ConclusionThe present study has advanced our understanding of the molecular regulatory networks contributing to muscle growth and breast muscle production and will contribute to the molecular breeding of chickens in the future.

目标提高胸肉产量是肉鸡行业的主要目标之一。在过去的几十年中，遗传选择和提高胸肌质量候选基因的鉴定取得了巨大的进展。方法在地面饲养系统中饲养 150 只 1 日龄雄性 817 肉鸡。在上市鸡龄为 50 d 时，宰杀 18 只健康的 817 肉鸡，收集每只鸡的左胸大肌样本进行 RNA-seq 测序。然后将肉鸡拔毛、开膛，取出整块胸肌并称重。胸肌产量按胸肌重量与内脏重量之比计算。结果 WGCNA分析将817只肉鸡胸肌中的所有共表达基因分为44个模块。在这些模块中，绿松石模块和天蓝3模块分别与胸肉产量呈显著正相关（r=0.78，p=1e-04）和负相关（r=-0.57，p=0.01）。进一步分析发现，几个参与肌肉结构和肌肉发育的中枢基因（如 DLX3、SH3RF2、TPM1、CAV3、MYF6 和 CFL2）被认为是乳肉产量的潜在调控因子。

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引用次数: 0

DOT1L is critical for adult vessel maintenance and functions. DOT1L 对成人血管的维护和功能至关重要。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-24 DOI: 10.5713/ab.23.0402

Heeji Lee, Dong Wook Han, Hyeonwoo La, C. Park, Kiye Kang, Ohbeom Kwon, Sang-Jun Uhm, Hyuk Song, J. Do, Y. Choi, Kwonho Hong

ObjectiveDOT1L is the only known histone H3K79 methyltransferase essential for the development of the embryonic cardiovascular system, including the heart, blood vessels, and lymphatic vessels, through transcriptional regulation. Our previous study demonstrated that Dot1l deletion results in aberrant lymphatic development and function. However, its precise function in the postnatal cardiovascular system remains unknown.MethodsUsing conditional and inducible Dot1l knockout (KO) mice, along with a reporter strain carrying the Geo gene at the Dot1l locus, DOT1L expression and its function in the vascular system during postnatal life were investigated. To assess vessel morphology and vascular permeability, we administered Latex or Evans blue dye to KO mice. In addition, in vitro tube formation and cell migration assays were performed using DOT1L-depleted human umbilical vein endothelial cells (HUVECs). Changes in the expression of vascular genes in HUVECs were measured by quantitative polymerase chain reaction.ResultsOur findings demonstrate that conditional Dot1l knockout in the Tg (Tie2-cre) strain results in abnormal blood vessel formation and lymphatic anomalies in the intestine. In a mouse model of Rosa26-creER-mediated inducible Dot1l knockout, we observed vascular phenotypes, including increased vascular permeability and brain hemorrhage, when DOT1L was deleted in adulthood. Additionally, DOT1L depletion in cultured HUVECs led to impaired cell migration and tube formation, likely due to altered gene transcription. These findings highlight the essential role of DOT1L in maintaining vascular integrity and function during embryonic development and postnatal life.ConclusionOur study revealed that DOT1L is required for the maintenance of adult vascular function through the regulation of gene expression.

目的DOT1L是唯一已知的组蛋白H3K79甲基转移酶，通过转录调控对胚胎心血管系统（包括心脏、血管和淋巴管）的发育至关重要。我们之前的研究表明，Dot1l 缺失会导致淋巴管发育和功能异常。方法利用条件性和诱导性 Dot1l 基因敲除（KO）小鼠以及携带 Dot1l 基因座 Geo 基因的报告基因品系，研究了 DOT1L 的表达及其在出生后血管系统中的功能。为了评估血管形态和血管通透性，我们给 KO 小鼠注射了乳胶或伊文思蓝染料。此外，我们还使用去除了 DOT1L 的人脐静脉内皮细胞（HUVECs）进行了体外管形成和细胞迁移试验。我们的研究结果表明，Tg（Tie2-cre）品系中条件性 Dot1l 基因敲除会导致肠道血管形成异常和淋巴异常。在Rosa26-creER介导的诱导性Dot1l基因敲除小鼠模型中，我们观察到了血管表型，包括在成年期删除DOT1L后血管通透性增加和脑出血。此外，在培养的 HUVECs 中删除 DOT1L 会导致细胞迁移和管形成受损，这可能是由于基因转录发生了改变。这些发现突显了 DOT1L 在胚胎发育和出生后维持血管完整性和功能方面的重要作用。

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引用次数: 0

Determination and prediction of amino acid digestibility in brown rice for growing-finishing pigs. 生长育成猪糙米中氨基酸消化率的测定与预测

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-24 DOI: 10.5713/ab.23.0455

Ouyang Qing, Rui Li, G. Feng, G. Hou, Xianji Jiang, Xiaojie Liu, Hui Tang, Cimi Long, Jie Yin, Yulong Yin

ObjectiveThe experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice.MethodsTwenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility.ResultsThe coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy (GE), in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine (Met), threonine (Thr) and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr and Trp were as following, SIDCP=﹣664.181+8.484×DM (R2=0.40), SIDLys=53.126+6.031×ether extract (EE) +0.893×thousand-kernel volume (R2=0.66), SIDThr=39.916+7.843×EE (R2=0.41) and SIDTrp=﹣361.588+4.891×DM+0.387×total starch (R2=0.85).ConclusionOverall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

方法将 22 头插管猪（初始体重：42.0±1.2 kg）分配到一个重复的 11×3 不完全拉丁方形设计中，包括无 N 日粮和 10 种糙米日粮。每个阶段包括 5 天的适应期和 2 天的回肠消化液收集期。结果10个糙米样品中除容重、干物质（DM）和总能（GE）外，所有物理特性和化学成分检测指标的变异系数均大于10%。糙米中CP、赖氨酸（Lys）、蛋氨酸（Met）、苏氨酸（Thr）和色氨酸（Trp）的SID分别为77.2%（62.6%至85.5%）、87.5%（80.3%至94.3%）、89.2%（78.9%至98.9%）、55.4%（46.1%至67.6%）和92.5%（86.3%至96.3%）。CP、Lys、Thr 和 Trp 的 SID 最佳预测方程如下：SIDCP=-664.181+8.484×DM（R2=0.40），SIDLys=53.126+6.031×乙醚提取物（EE）+0.893×千粒重（R2=0.66），SIDThr=39.916+7.843×EE（R2=0.总之，10 种糙米来源之间存在很大差异，千粒重、DM、EE 和总淀粉可作为预测 CP 和 AA SID 的关键指标。

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引用次数: 0

Genome-wide profiling of histone acetylation enhancer of fatty liver hemorrhagic syndrome in laying hens. 蛋鸡脂肪肝出血性综合征组蛋白乙酰化增强子的全基因组图谱分析。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-24 DOI: 10.5713/ab.23.0423

Yi Wang, Shuwen Chen, Xue Min, Jinhu Ma, Xinrui Yi, Xuejin Lu, Xinyu Li, Meizi Zhu, Jin Peng, Yunshu Tang, Yaling Zhu

ObjectiveRare study of the non-coding and regulatory regions of the genome limits our ability to decode the mechanisms of fatty liver hemorrhage syndrome (FLHS) in chickens.MethodHerein, we constructed the HFD-induced FLHS chicken model to investigate the genome-wide active enhancers and transcriptome by H3K27ac target chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-Seq) profiles of normal and FLHS liver tissues. Concurrently, an integrative analysis combining ChIP-seq with RNA-Seq and a comparative analysis with chicken FLHS, rat Non-alcoholic fatty liver disease (NAFLD) and human NAFLD at the transcriptome level revealed the enhancer target genes and conservative genes involved in metabolic processes.ResultsIn total, 56 and 199 peak-genes were identified in upregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥ 0.5 & log2(FoldChange) ≥ 1) (PP) and downregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥ 0.5 & log2(FoldChange) ≤ -1)(PN), respectively; then we screened key regulatory targets mainly distributing in lipid metabolism (PCK1, APOA4, APOA1, INHBE) and apoptosis (KIT, NTRK2) together with MAPK and PPAR signaling pathway in FLHS. Intriguingly, PCK1 was also significantly covered in up-regulated super-enhancers (SEs), which further implied the vital role of PCK1 during the development of FLHS.ConclusionTogether, our studies provided new insights into the pathogenesis and potential therapy biomarkers of FLHS.

方法我们构建了高密度脂蛋白胆固醇（HFD）诱导的鸡脂肪肝模型，通过H3K27ac靶染色质免疫沉淀测序（ChIP-seq）和RNA测序（RNA-Seq）研究正常肝组织和脂肪肝肝组织的全基因组活性增强子和转录组。同时，将 ChIP-seq 与 RNA-Seq 结合起来进行综合分析，并在转录组水平上与鸡非酒精性脂肪肝、大鼠非酒精性脂肪肝和人类非酒精性脂肪肝进行比较分析，发现了增强子靶基因和参与代谢过程的保守基因。5 & log2(FoldChange) ≥ 1) (PP)和受 H3K27ac 正调控的下调峰基因 (Cor (peak-gene correlation) ≥ 0.5 & log2(FoldChange) ≤ -1) (PN)；然后筛选出 FLHS 中主要分布于脂质代谢（PCK1、APOA4、APOA1、INHBE）和细胞凋亡（KIT、NTRK2）以及 MAPK 和 PPAR 信号通路的关键调控靶点。结论我们的研究为FLHS的发病机制和潜在治疗生物标志物提供了新的见解。

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引用次数: 0

Bta-miR-365-3p-targeted FKBP5 participates in the AMPK/mTOR signaling pathway in the regulation of preadipocyte differentiation in cattle. Bta-miR-365-3p 靶向 FKBP5 参与 AMPK/mTOR 信号通路对牛前脂肪细胞分化的调控。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-24 DOI: 10.5713/ab.23.0328

Mengdi Chen, Congcong Zhang, Zewen Wu, Siwei Guo, Wenfa Lv, Jixuan Song, Beibei Hao, Jinhui Bai, Xinxin Zhang, Hongyan Xu, Guangjun Xia

ObjectiveMicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood.MethodsWe identified bta-miR-365-3p, which specifically targets the 3'untranslated region (3'UTR) of the FK506-binding protein 5 (FKBP5),and verified its mechanisms for regulating expression and involvement in adipogenesis.ResultsIn this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-365-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBPα and PPARγ. The dual-luciferase reporter system further validated the targeting relationship between bta-miR-365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by qRT-PCR and Western blot. Overexpression of bta-miR-365-3p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. AMPK/mTOR signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-365-3p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results.ConclusionIn conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.

目的微小RNA（miRNA）是一种内源性非编码RNA，可在哺乳动物前脂肪细胞分化的转录后调控中发挥作用。方法我们鉴定了特异性靶向 FK506 结合蛋白 5（FKBP5）3'非翻译区（3'UTR）的 bta-miR-365-3p，并验证了其调控表达和参与脂肪生成的机制。结果在这项研究中，我们发现过表达 bta-miR-365-3p 能显著减少脂肪细胞中的脂质积累和甘油三酯含量。与抑制 bta-miR-365-3p 组相比，过表达 bta-miR-365-3p 可抑制脂肪细胞分化相关基因 C/EBPα 和 PPARγ 的表达。双荧光素酶报告系统进一步验证了 bta-miR-365-3p 与 FKBP5 的靶向关系。通过 qRT-PCR 和 Western 印迹检测了 FKBP5 mRNA 和蛋白的表达。过表达bta-miR-365-3p会显著下调FKBP5的表达，而抑制bta-miR-365-3p则相反，表明bta-miR-365-3p对FKBP5有负向调控作用。AMPK/mTOR信号通路与细胞生长调控密切相关，参与了牛脂肪细胞的发育。在本研究中，过表达 bta-miR-365-3p 能显著抑制 AMPK、mTOR 和 SREBP1 基因的 mRNA 和蛋白表达，而抑制 bta-miR-365-3p 的表达则与上述结果相反。结论综上所述，这些结果表明，bta-miR-365-3p 可能通过靶向 FKBP5 基因参与了 AMPK/mTOR 信号通路对延边黄牛前脂肪细胞分化的调控。

{"title":"Bta-miR-365-3p-targeted FKBP5 participates in the AMPK/mTOR signaling pathway in the regulation of preadipocyte differentiation in cattle.","authors":"Mengdi Chen, Congcong Zhang, Zewen Wu, Siwei Guo, Wenfa Lv, Jixuan Song, Beibei Hao, Jinhui Bai, Xinxin Zhang, Hongyan Xu, Guangjun Xia","doi":"10.5713/ab.23.0328","DOIUrl":"https://doi.org/10.5713/ab.23.0328","url":null,"abstract":"Objective\u0000MicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood.\u0000\u0000\u0000Methods\u0000We identified bta-miR-365-3p, which specifically targets the 3'untranslated region (3'UTR) of the FK506-binding protein 5 (FKBP5),and verified its mechanisms for regulating expression and involvement in adipogenesis.\u0000\u0000\u0000Results\u0000In this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-365-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBPα and PPARγ. The dual-luciferase reporter system further validated the targeting relationship between bta-miR-365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by qRT-PCR and Western blot. Overexpression of bta-miR-365-3p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. AMPK/mTOR signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-365-3p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results.\u0000\u0000\u0000Conclusion\u0000In conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140662162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genome-wide association study on growth traits of Korean commercial pig breeds using Bayesian methods. 利用贝叶斯方法对韩国商品猪品种的生长性状进行全基因组关联研究。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-15 DOI: 10.5713/ab.23.0443

J. Jung, Sang Min Lee, Sang-Hyon Oh

ObjectiveThis study aims to identify the significant regions and candidate genes of growth-related traits (adjusted backfat thickness; ABF, average daily gain; ADG, and days to 90 kg body weight; DAYS90) in Korean commercial GGP pig (Duroc, Landrace, and Yorkshire) populations.ResultsMethods: A genome-wide association study was performed using single-nucleotide polymorphism (SNP) markers for imputation to Illumina PorcineSNP60. The BayesB method was applied to calculate thresholds for the significance of SNP markers. The identified windows were considered significant if they explained ≥ 1% genetic variance.ResultsA total of 28 window regions were related to genetic growth effects. Bayesian GWAS revealed 28 significant genetic regions including 52 informative SNPs associated with growth traits (ABF, ADG, DAYS90) in Duroc, Landrace, and Yorkshire pigs, with genetic variance ranging from 1.00% to 5.46%. Additionally, 14 candidate genes with previous functional validation were identified for these traits.ConclusionThe results enhance our understanding of genetic architecture and our potential to genetically improve pigs. SNPs within the identified regions could prove valuable for future marker-assisted or genomic selection in pig breeding programs.

目的：本研究旨在确定韩国商品GGP猪（杜洛克猪、兰氏猪和约克夏猪）种群中生长相关性状（调整背膘厚（ABF）、平均日增重（ADG）和90千克体重天数（DAYS90））的显著区域和候选基因：使用单核苷酸多态性（SNP）标记对Illumina PorcineSNP60进行归因，开展了一项全基因组关联研究。采用 BayesB 方法计算 SNP 标记的显著性阈值。结果共有 28 个窗口区域与遗传生长效应有关。贝叶斯 GWAS 发现了 28 个显著的遗传区域，包括 52 个与杜洛克猪、陆地猪和约克夏猪生长性状（ABF、ADG、DAYS90）相关的信息 SNPs，遗传变异范围为 1.00% 至 5.46%。此外，还为这些性状鉴定了 14 个先前经过功能验证的候选基因。已鉴定区域内的 SNP 对未来猪育种计划中的标记辅助选择或基因组选择很有价值。

{"title":"A genome-wide association study on growth traits of Korean commercial pig breeds using Bayesian methods.","authors":"J. Jung, Sang Min Lee, Sang-Hyon Oh","doi":"10.5713/ab.23.0443","DOIUrl":"https://doi.org/10.5713/ab.23.0443","url":null,"abstract":"Objective\u0000This study aims to identify the significant regions and candidate genes of growth-related traits (adjusted backfat thickness; ABF, average daily gain; ADG, and days to 90 kg body weight; DAYS90) in Korean commercial GGP pig (Duroc, Landrace, and Yorkshire) populations.\u0000\u0000\u0000Results\u0000Methods: A genome-wide association study was performed using single-nucleotide polymorphism (SNP) markers for imputation to Illumina PorcineSNP60. The BayesB method was applied to calculate thresholds for the significance of SNP markers. The identified windows were considered significant if they explained ≥ 1% genetic variance.\u0000\u0000\u0000Results\u0000A total of 28 window regions were related to genetic growth effects. Bayesian GWAS revealed 28 significant genetic regions including 52 informative SNPs associated with growth traits (ABF, ADG, DAYS90) in Duroc, Landrace, and Yorkshire pigs, with genetic variance ranging from 1.00% to 5.46%. Additionally, 14 candidate genes with previous functional validation were identified for these traits.\u0000\u0000\u0000Conclusion\u0000The results enhance our understanding of genetic architecture and our potential to genetically improve pigs. SNPs within the identified regions could prove valuable for future marker-assisted or genomic selection in pig breeding programs.","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140699367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of relevant differential genes to the divergent devel-opment of pectoral muscle in ducks by transcriptomic analysis. 通过转录组分析鉴定鸭胸肌发育差异的相关差异基因

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-01 DOI: 10.5713/ab.23.0505

Fan Li, Zongliang He, Yinglin Lu, Jing Zhou, Heng Cao, Xingyu Zhang, Hongjie Ji, Kunpeng Lv, Debing Yu, Minli Yu

ObjectiveThe objective of this study was to identify candidate genes that play im-portant roles in skeletal muscle development in ducks.MethodsIn this study, we investigated the transcriptional sequencing of embryonic pectoral muscles from two specialized line LCA and LCC ducks which were devel-oped from Liancheng White ducks (female) and Cherry Valley ducks (male) F6 hybrid population. In addition, prediction of target genes for the differentially expressed mRNAs was conducted and the enriched gene ontology (GO) terms and Kyoto En-cyclopedia of Genes and Genomes (KEGG) signaling pathways were further analyzed. Finally, a protein-to-protein interaction (PPI) network was analyzed by using the tar-get genes to gain insights into their potential functional association.ResultsA total of 1428 differentially expressed genes (DEGs) with 762 being up-regulated genes and 666 being down-regulated genes in pectoral muscle of LCA and LCC ducks identified by RNA-seq (p < 0.05). Meanwhile, 23 GO terms in the down-regulated genes and 75 GO terms in up-regulated genes were significantly en-riched (p < 0.05). Furthermore, the top 5 most enriched pathways were ECM-receptor interaction, fatty acid degradation, pyruvate degradation, PPAR signaling pathway, and glycolysis/gluconeogenesis. Finally, the candidate genes including Integrin b3 (Itgb3), Pyruvate kinase M1/2 (Pkm), Insulin-like growth factor 1 (Igf1), glu-cose-6-phosphate isomerase（Gpi）, GABA type A receptor-associated protein-like 1(Gabarapl1), and Thyroid hormone receptor beta (Thrb) showed the most expression difference, and then were selected to verification by qRT-PCR. The result of qRT-PCR was consistent with that of transcriptome sequencing.ConclusionThis study provided information of molecular mechanisms underlying the developmental differences in skeletal muscles between specialized duck lines.

方法对由连城白鸭（雌性）和樱桃谷鸭（雄性）F6杂交培育的两个特化系LCA和LCC鸭的胚胎胸肌进行转录测序。此外，还对差异表达的 mRNA 的靶基因进行了预测，并进一步分析了富集的基因本体（GO）术语和京都基因与基因组百科（KEGG）信号通路。结果 RNA-seq共鉴定出1428个差异表达基因（DEGs），其中762个为上调基因，666个为下调基因（P < 0.05）。同时，下调基因中的23个GO术语和上调基因中的75个GO术语被显著富集（P < 0.05）。此外，富集最多的前 5 个通路分别是 ECM-受体相互作用、脂肪酸降解、丙酮酸降解、PPAR 信号通路和糖酵解/糖元生成。最后，包括整合素b3（Itgb3）、丙酮酸激酶M1/2（Pkm）、类胰岛素生长因子1（Igf1）、葡萄糖-6-磷酸异构酶（Gpi）、GABA A型受体相关蛋白样1（Gabarapl1）和甲状腺激素受体β（Thrb）在内的候选基因的表达差异最大，因此被选中进行qRT-PCR验证。结论本研究提供了特化鸭品系间骨骼肌发育差异的分子机制信息。

{"title":"Identification of relevant differential genes to the divergent devel-opment of pectoral muscle in ducks by transcriptomic analysis.","authors":"Fan Li, Zongliang He, Yinglin Lu, Jing Zhou, Heng Cao, Xingyu Zhang, Hongjie Ji, Kunpeng Lv, Debing Yu, Minli Yu","doi":"10.5713/ab.23.0505","DOIUrl":"https://doi.org/10.5713/ab.23.0505","url":null,"abstract":"Objective\u0000The objective of this study was to identify candidate genes that play im-portant roles in skeletal muscle development in ducks.\u0000\u0000\u0000Methods\u0000In this study, we investigated the transcriptional sequencing of embryonic pectoral muscles from two specialized line LCA and LCC ducks which were devel-oped from Liancheng White ducks (female) and Cherry Valley ducks (male) F6 hybrid population. In addition, prediction of target genes for the differentially expressed mRNAs was conducted and the enriched gene ontology (GO) terms and Kyoto En-cyclopedia of Genes and Genomes (KEGG) signaling pathways were further analyzed. Finally, a protein-to-protein interaction (PPI) network was analyzed by using the tar-get genes to gain insights into their potential functional association.\u0000\u0000\u0000Results\u0000A total of 1428 differentially expressed genes (DEGs) with 762 being up-regulated genes and 666 being down-regulated genes in pectoral muscle of LCA and LCC ducks identified by RNA-seq (p < 0.05). Meanwhile, 23 GO terms in the down-regulated genes and 75 GO terms in up-regulated genes were significantly en-riched (p < 0.05). Furthermore, the top 5 most enriched pathways were ECM-receptor interaction, fatty acid degradation, pyruvate degradation, PPAR signaling pathway, and glycolysis/gluconeogenesis. Finally, the candidate genes including Integrin b3 (Itgb3), Pyruvate kinase M1/2 (Pkm), Insulin-like growth factor 1 (Igf1), glu-cose-6-phosphate isomerase（Gpi）, GABA type A receptor-associated protein-like 1(Gabarapl1), and Thyroid hormone receptor beta (Thrb) showed the most expression difference, and then were selected to verification by qRT-PCR. The result of qRT-PCR was consistent with that of transcriptome sequencing.\u0000\u0000\u0000Conclusion\u0000This study provided information of molecular mechanisms underlying the developmental differences in skeletal muscles between specialized duck lines.","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140766050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of gold and green kiwifruit juices on the physicochemical properties and tenderness of pork loin and antioxidant activity during incubation (24 hrs) in a pork model system. 在猪肉模型系统中，金猕猴桃汁和绿猕猴桃汁对猪里脊肉理化特性和嫩度的影响，以及在培养过程（24 小时）中的抗氧化活性。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-01 DOI: 10.5713/ab.23.0410

Ha Eun Kim, K. Chin

ObjectiveAlthough pork loins is not a tough meat, they need to develop for meat products with a soft texture for the elderly. This study focused on the physicochemical properties and tenderness characteristics of pork loin injected with green kiwifruit juice (GRJ) and gold kiwifruit juice (GOJ) during various incubation times. In addition, the antioxidant activities of hydrolysate derived from the hydrolysis of pork loin by kiwifruit juice protease were evaluated.MethodsThe pork loin was injected with 10% and 20% GRJ and GOJ, under various incubation times (0, 4, 8, and 24 h). Then, the physicochemical properties and tenderness of pork loins. 2,2- diphenyl-1-picrylhydrazyl (DPPH) and reducing power were conducted to determine hydrolysate's antioxidant activities derived from pork loin's hydrolysis by kiwifruit juice protease.ResultsGRJ had greater tenderizing ability than GOJ, even at the 10% addition. When kiwifruit juice was injected into pork loin, the tenderness increased with increasing incubation time. This was confirmed by the decrease in intensity of the myosin heavy chain (MHC) band in SDS-PAGE. In particular, the MHC band decreased at 8 h for both 10% GRJ and 20% GOJ and at 4 h for 20% GRJ alone. The highest myofibril fragmentation index and peptide solubility were observed in pork loin treated with 20% GRJ compared to the other treatments during incubation. The 10% GRJ and 20% GOJ treatments showed similar levels of antioxidant activity of the protein hydrolysates in pork loin, and 20% GRJ showed the highest activity among the treatments.ConclusionKiwifruit juice had protease activity, and GRJ was more useful for the tenderness of meat products than GOJ. Thus, GRJ at 10% could be a potential agent to tenderize and enrich the natural antioxidant activity through the proteolysis of pork loin.

目标虽然猪里脊肉并不是一种坚硬的肉类，但他们需要开发出质地柔软的肉类产品，以满足老年人的需求。本研究重点考察了猪里脊肉在不同培养时间内注入绿猕猴桃汁（GRJ）和金猕猴桃汁（GOJ）后的理化特性和嫩度特征。此外，还评估了猕猴桃汁蛋白酶水解猪里脊肉所产生的水解物的抗氧化活性。方法在不同的培养时间（0、4、8 和 24 h）下，猪里脊肉分别注入 10% 和 20% 的绿猕猴桃汁和金猕猴桃汁。然后，对猪里脊肉的理化性质和嫩度进行检测。然后，对猪里脊肉的理化性质和嫩度、2,2-二苯基-1-苦基肼（DPPH）和还原力进行测定，以确定猕猴桃汁蛋白酶水解猪里脊肉所产生的水解物的抗氧化活性。将猕猴桃汁注入猪里脊肉后，随着培养时间的延长，嫩度也会增加。SDS-PAGE 中肌球蛋白重链（MHC）条带强度的降低证实了这一点。特别是，10% GRJ 和 20% GOJ 的 MHC 带在 8 小时后减少，而仅 20% GRJ 的 MHC 带在 4 小时后减少。在培养过程中，与其他处理相比，用 20% GRJ 处理的猪里脊肉的肌原纤维碎裂指数和肽溶解度最高。结论猕猴桃汁具有蛋白酶活性，与 GOJ 相比，GRJ 更有助于提高肉制品的嫩度。因此，10% 的猕猴桃汁可作为一种潜在的添加剂，通过分解猪里脊肉的蛋白来嫩化肉质并增强其天然抗氧化活性。

{"title":"Effects of gold and green kiwifruit juices on the physicochemical properties and tenderness of pork loin and antioxidant activity during incubation (24 hrs) in a pork model system.","authors":"Ha Eun Kim, K. Chin","doi":"10.5713/ab.23.0410","DOIUrl":"https://doi.org/10.5713/ab.23.0410","url":null,"abstract":"Objective\u0000Although pork loins is not a tough meat, they need to develop for meat products with a soft texture for the elderly. This study focused on the physicochemical properties and tenderness characteristics of pork loin injected with green kiwifruit juice (GRJ) and gold kiwifruit juice (GOJ) during various incubation times. In addition, the antioxidant activities of hydrolysate derived from the hydrolysis of pork loin by kiwifruit juice protease were evaluated.\u0000\u0000\u0000Methods\u0000The pork loin was injected with 10% and 20% GRJ and GOJ, under various incubation times (0, 4, 8, and 24 h). Then, the physicochemical properties and tenderness of pork loins. 2,2- diphenyl-1-picrylhydrazyl (DPPH) and reducing power were conducted to determine hydrolysate's antioxidant activities derived from pork loin's hydrolysis by kiwifruit juice protease.\u0000\u0000\u0000Results\u0000GRJ had greater tenderizing ability than GOJ, even at the 10% addition. When kiwifruit juice was injected into pork loin, the tenderness increased with increasing incubation time. This was confirmed by the decrease in intensity of the myosin heavy chain (MHC) band in SDS-PAGE. In particular, the MHC band decreased at 8 h for both 10% GRJ and 20% GOJ and at 4 h for 20% GRJ alone. The highest myofibril fragmentation index and peptide solubility were observed in pork loin treated with 20% GRJ compared to the other treatments during incubation. The 10% GRJ and 20% GOJ treatments showed similar levels of antioxidant activity of the protein hydrolysates in pork loin, and 20% GRJ showed the highest activity among the treatments.\u0000\u0000\u0000Conclusion\u0000Kiwifruit juice had protease activity, and GRJ was more useful for the tenderness of meat products than GOJ. Thus, GRJ at 10% could be a potential agent to tenderize and enrich the natural antioxidant activity through the proteolysis of pork loin.","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140785920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota derived from fecal microbiota transplantation enhances body weight of Mimas squabs. 从粪便微生物群移植中提取的肠道微生物群可提高米马斯乳鸽的体重。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-01 DOI: 10.5713/ab.23.0475

Jing Ren, Yumei Li, Hongyu Ni, Yan Zhang, Puze Zhao, Qingxing Xiao, Xiaoqing Hong, Ziyi Zhang, Yijing Yin, Xiaohui Li, Yonghong Zhang, Yuwei Yang

ObjectiveCompared to Mimas pigeons, Shiqi pigeons exhibit greater tolerance to coarse feeding because of their abundant gut microbiota. Here, to investigate the potential of utilizing intestinal flora derived from Shiqi pigeons, the intestinal flora and body indices of Mimas squabs were evaluated after fecal microbiota transplantation (FMT) from donors.MethodsA total of 90 one-day-old squabs were randomly divided into the control group (CON), the low-concentration group (LC) and the high-concentration group (HC): gavaged with 200 μL of bacterial solution at concentrations of 0, 0.1 and 0.2 g/15 mL, respectively.ResultsThe results suggested that FMT improved the body weight of Mimas squabs in the HC and LC groups (p < 0.01), and 0.1 g/15 mL was the optimal dose during FMT. After 16S rRNA sequencing was performed, compared to those in the CON group, the abundance levels of microflora, especially Lactobacillus, Muribaculaceae and Megasphaera (p < 0.05), in the FMT-treated groups were markedly greater. Random forest analysis indicated that the main functions of key microbes involve pathways associated with metabolism, further illustrating their important role in the host body.ConclusionFMT has been determined to be a viable method for augmenting the weight and intestinal microbiota of squabs, representing a unique avenue for enhancing the economic feasibility of squab breeding.

目的与米马斯乳鸽相比，石岐乳鸽因其丰富的肠道微生物群而表现出更强的耐粗饲性。为了研究利用石岐鸽肠道菌群的潜力，我们对从供体获得粪便微生物群移植（FMT）后的乳鸽进行了肠道菌群和身体指数评估。结果结果表明，粪便微生物群移植可改善 HC 组和 LC 组乳鸽的体重（p < 0.01），0.1 g/15 mL 是粪便微生物群移植的最佳剂量。在进行 16S rRNA 测序后，与 CON 组相比，FMT 治疗组的微生物菌群，尤其是乳酸杆菌、Muribaculaceae 和 Megasphaera 的丰度水平明显更高（p < 0.05）。随机森林分析表明，关键微生物的主要功能涉及与新陈代谢有关的途径，这进一步说明了它们在宿主体内的重要作用。

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引用次数: 0

Effects of dietary inclusion of Moringa oleifera leaf meal on growth performance of Muscovy ducklings (Cairina moschata). 辣木叶粉对番鸭生长性能的影响。

IF 2.2 2区农林科学 Q1 Veterinary

Animal Bioscience

Pub Date : 2024-04-01 Epub Date: 2023-11-01 DOI: 10.5713/ab.23.0208

Assem M Safwat, Luis Sarmiento-Franco, Enass Abd El-Khalek, Bahaa M Abou-Shehema, Osama A Hassan, Asmaa Sh Elnaggar

Objective: The current experiment was performed to investigate the influence of different dietary levels of Moringa oleifera leaf meal (MOLM) on productive performance, nutrient digestibility, blood parameters, immune response, caecal microbiota, and carcass characteristics of Muscovy ducks (Cairina moschata) during 7 to 63 d of age.

Methods: A total of 240 unsexed 7-d-old ducklings were distributed into five (treatment) groups; each one contained six replicates with eight ducklings each. Birds of the first group were fed basal diet without MOLM and served as control, while the other four groups were fed basal diet with 0.25%, 0.50%, 1.0%, and 2.0% MOLM inclusion level, respectively.

Results: The obtained results revealed that including MOLM in the diets significantly improved body weight, body weight gain, feed conversion ratio and economic efficiency compared with the control group. Among the different MOLM inclusion treatments, increasing MOLM inclusion level decreased (p<0.05) such previous parameters. Decreasing MOLM inclusion levels in duckling diets increased (p>0.05) the digestibility of organic matter, crude protein, ether extract, and nitrogen free extract, however all MOLM treatments were significantly higher than the control group. Results also revealed that feeding ducks lower MOLM inclusion levels (0.25% or 0.50%) improved blood parameters (p<0.05) compared with the higher inclusion levels (1.0% or 2.0% MOLM) and the control group. Ducks fed different MOLM levels had significantly higher phagocyte index and activity, immunoglobulin G (IgG), IgM, total antioxidant capacity, glutathione peroxidase activity, and superoxide dismutase activity compared with control group.

Conclusion: Despite the beneficial effects of all MOLM treatments on growth performance, nutrient digestibility, physiological status, and immune response of duckling, the increasing MOLM inclusion level in the diet had deleterious effects on such studied traits, consequently 0.25% was the best MOLM inclusion level in duckling diets.

目的：研究不同日粮水平辣木叶粉（MOLM）对7～63d番鸭生产性能、营养物质消化率、血液参数、免疫反应、盲肠微生物群和胴体性状的影响。方法：将240只7日龄雏鸭随机分为5组（处理组）；每只包含六个复制品，每个复制品有八只小鸭子。第一组的鸟被喂食不含MOLM的基础日粮并作为对照，而其他四组的鸟则被喂食分别含有0.25%、0.50、1.0和2.0%MOLM的基本日粮。结果：与对照组相比，日粮中添加MOLM显著提高了体重、增重、饲料转化率和经济效益。在不同的MOLM包合处理中，增加MOLM包埋水平降低了有机物、粗蛋白、乙醚提取物和无氮提取物的消化率（p0.05），但所有MOLM处理均显著高于对照组。结果还显示，喂食鸭子降低了MOLM含量（0.25%或0.50%），改善了血液参数（P结论：尽管所有MOLM处理对雏鸭的生长性能、营养物质消化率、生理状态和免疫反应都有有益影响，但日粮中MOLM含量的增加对这些研究性状有不利影响，因此0.25%是雏鸭日粮中最佳的MOLM含量。

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引用次数: 0