{"title":"[Kinetic and chemical study of succinyl papain].","authors":"H G Löffler, F Schneider","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1490-7"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oxygen consumption by liver and kidney slices of Uromastix hardwickii.","authors":"H S Athar, S N Hasnain, M Zain-ul-Abedin","doi":"10.1515/znb-1972-1217","DOIUrl":"https://doi.org/10.1515/znb-1972-1217","url":null,"abstract":"","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1529-30"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1972-1217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1. Initial glycogen levels in the liver were found to be significantly higher than in the kidneys of the two species studied. The glycogen levels in kidney were however considerably higher than in some mammalian species. 2. Total reducing capacity, true glucose, saccharoid fraction and titratable acidity increase gradually with concomitant decrease in the glycogen levels when liver and kidney homogenates of uromastix and liver homogenate of varanus were incubated for a period of 4 hours. 3. Total reducing capacity, true glucose and the titratable acidity increase gradually, while the glycogen and the saccharoid fraction remain unchanged when the kidney homogenates of varanus are incubated for 4 hours. 4. Total reducing capacity, true glucose and saccharoid fraction in the blood of uromastix are higher than in varanus. The blood glucose levels in both the species are higher than found in the mammals
{"title":"Studies on carbohydrate metabolism in lizards.","authors":"H S Athar, S N Hasnain, M Zain-ul-Abedin","doi":"10.1515/znb-1972-1218","DOIUrl":"https://doi.org/10.1515/znb-1972-1218","url":null,"abstract":"1. Initial glycogen levels in the liver were found to be significantly higher than in the kidneys of the two species studied. The glycogen levels in kidney were however considerably higher than in some mammalian species. 2. Total reducing capacity, true glucose, saccharoid fraction and titratable acidity increase gradually with concomitant decrease in the glycogen levels when liver and kidney homogenates of uromastix and liver homogenate of varanus were incubated for a period of 4 hours. 3. Total reducing capacity, true glucose and the titratable acidity increase gradually, while the glycogen and the saccharoid fraction remain unchanged when the kidney homogenates of varanus are incubated for 4 hours. 4. Total reducing capacity, true glucose and saccharoid fraction in the blood of uromastix are higher than in varanus. The blood glucose levels in both the species are higher than found in the mammals","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1531-5"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1972-1218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Stimulation of embryonic rat cells in culture by calf-serum. IV. Isolation of an Active Protein and Partial Purification of a second Stimulating Fraction from fetal calf-serum].","authors":"J Veser, W Frank","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1573-4"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Cancer cell kinetics and cancer multi-step therapy].","authors":"M von Ardenne","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1547-66"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[IR, Raman, 1 H-NMR spectra and acidity constants of the cytostatic Hadacidin and its mono alkali salts].","authors":"H P Fritz, O von Stetten","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1457-62"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3H-thymidine incorporation by secondary cultures of embryonic rat cells and by cultures of a permanent rat cell line is strongly inhibited a few minutes after addition of hydroxyurea (HU) to the culture medium (2 × 10-3 ᴍ and 5 × 10-4 ᴍ, resp.); normal embryonic rat cells are considerably less sensitive to the drug than are permanent cells. About 76 percent of the population of a secondary culture can be synchronized at the beginning of the S-period, if the cells at first are accumulated in G1 phase by incubation in serum-free medium and subsequently are stimulated by the addition of ten percent calf serum in the presence of 2 × 10-3 ᴍ HU. The size of DNA replicated in the presence of HU has been analysed by centrifugation in alkaline sucrose gradients. The results indicate that under these conditions DNA subunits are synthesized with sedimentation coefficients between 21S and 30S. These intermediates cannot be joined together, probably because a ligase is inhibited by HU. Secondary culture incubated in medium-containing 1 × 10-4 ᴍ HU incorporate twice as much 3H-thymidine as do control cells in HU-free medium. This is due to an increased number of cells in the S phase, as was shown by autoradiography. The kinetics of thymidine incorporation indicate that the duration of G1 phase is shortened by small doses of HU
{"title":"[The effect of hydroxyurea on DNA synthesis of embryonic rat cells in culture].","authors":"R Manso-Martinez, W Frank","doi":"10.1515/znb-1972-1214","DOIUrl":"https://doi.org/10.1515/znb-1972-1214","url":null,"abstract":"3H-thymidine incorporation by secondary cultures of embryonic rat cells and by cultures of a permanent rat cell line is strongly inhibited a few minutes after addition of hydroxyurea (HU) to the culture medium (2 × 10-3 ᴍ and 5 × 10-4 ᴍ, resp.); normal embryonic rat cells are considerably less sensitive to the drug than are permanent cells. About 76 percent of the population of a secondary culture can be synchronized at the beginning of the S-period, if the cells at first are accumulated in G1 phase by incubation in serum-free medium and subsequently are stimulated by the addition of ten percent calf serum in the presence of 2 × 10-3 ᴍ HU. The size of DNA replicated in the presence of HU has been analysed by centrifugation in alkaline sucrose gradients. The results indicate that under these conditions DNA subunits are synthesized with sedimentation coefficients between 21S and 30S. These intermediates cannot be joined together, probably because a ligase is inhibited by HU. Secondary culture incubated in medium-containing 1 × 10-4 ᴍ HU incorporate twice as much 3H-thymidine as do control cells in HU-free medium. This is due to an increased number of cells in the S phase, as was shown by autoradiography. The kinetics of thymidine incorporation indicate that the duration of G1 phase is shortened by small doses of HU","PeriodicalId":78857,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie","volume":"27 12","pages":"1500-7"},"PeriodicalIF":0.0,"publicationDate":"1972-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1972-1214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15254215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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