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Promoting Positive Development Among Racially and Ethnically Marginalized Youth: Advancing a Novel Model of Natural Mentoring. 促进种族和民族边缘化青少年的积极发展:推进自然指导的新模式。
IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-01 Epub Date: 2024-07-02 DOI: 10.1146/annurev-clinpsy-080822-045011
Noelle M Hurd

Racism and other forms of oppression threaten the well-being of racially and ethnically marginalized youth. Models of risk and resilience for marginalized youth have stressed the importance of addressing contextual and structural risk while emphasizing promotive factors such as cultural capital within their communities. Increasingly, research has focused on collective antiracist action as a form of coping with structural oppression. Importantly, supportive intergenerational relationships that develop within youths' everyday contexts may play a key role in catalyzing and reinforcing youths' engagement in antiracist action. This review advances a novel model for understanding how supportive nonparental adults from youths' everyday lives (i.e., natural mentors) influence youths' positive developmental outcomes and participation in antiracist action and how collective antiracist action, in turn, fosters liberation and racial justice. The creation of a more just and equitable society contributes to positive development among racially and ethnically marginalized youth.

种族主义和其他形式的压迫威胁着种族和民族边缘化青年的福祉。边缘化青年的风险和复原力模型强调了解决背景和结构性风险的重要性,同时强调了其社区内的文化资本等促进因素。越来越多的研究关注集体反种族主义行动,将其作为应对结构性压迫的一种形式。重要的是,在青少年日常环境中形成的支持性代际关系可能会在促进和加强青少年参与反种族主义行动方面发挥关键作用。本综述提出了一种新的模式,用于理解青少年日常生活中具有支持性的非父母成年人(即自然导师)如何影响青少年的积极发展成果和对反种族主义行动的参与,以及反种族主义集体行动如何反过来促进解放和种族正义。建立一个更加公正和公平的社会有助于种族和民族边缘化青年的积极发展。临床心理学年度评论》第 20 卷的最终在线出版日期预计为 2024 年 5 月。修订后的预计日期请参见 http://www.annualreviews.org/page/journal/pubdates。
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引用次数: 0
Mobile Health Interventions for Substance Use Disorders. 针对药物使用障碍的移动健康干预。
IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-01 Epub Date: 2024-07-02 DOI: 10.1146/annurev-clinpsy-080822-042337
Michael S Businelle, Olga Perski, Emily T Hébert, Darla E Kendzor

Substance use disorders (SUDs) have an enormous negative impact on individuals, families, and society as a whole. Most individuals with SUDs do not receive treatment because of the limited availability of treatment providers, costs, inflexible work schedules, required treatment-related time commitments, and other hurdles. A paradigm shift in the provision of SUD treatments is currently underway. Indeed, with rapid technological advances, novel mobile health (mHealth) interventions can now be downloaded and accessed by those that need them anytime and anywhere. Nevertheless, the development and evaluation process for mHealth interventions for SUDs is still in its infancy. This review provides a critical appraisal of the significant literature in the field of mHealth interventions for SUDs with a particular emphasis on interventions for understudied and underserved populations. We also discuss the mHealth intervention development process, intervention optimization, and important remaining questions.

药物滥用障碍 (SUD) 对个人、家庭和整个社会都有巨大的负面影响。由于治疗提供者有限、费用高昂、工作时间安排不灵活、治疗相关的时间要求以及其他障碍,大多数 SUD 患者都没有接受治疗。目前,提供药物滥用治疗的模式正在发生转变。事实上,随着技术的飞速发展,新颖的移动医疗(mHealth)干预措施现在可以随时随地下载并提供给有需要的人。然而,针对 SUDs 的移动医疗干预措施的开发和评估过程仍处于起步阶段。本综述对移动医疗干预 SUDs 领域的重要文献进行了批判性评估,并特别强调了针对研究不足和服务欠缺人群的干预措施。我们还讨论了移动保健干预措施的开发过程、干预措施的优化以及重要的遗留问题。临床心理学年度评论》第 20 卷的最终在线出版日期预计为 2024 年 5 月。有关修订后的预计日期,请参阅 http://www.annualreviews.org/page/journal/pubdates。
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引用次数: 0
Missing Data Analysis. 缺失数据分析。
IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-01 Epub Date: 2024-07-02 DOI: 10.1146/annurev-clinpsy-080822-051727
Roderick J Little

Methods for handling missing data in clinical psychology studies are reviewed. Missing data are defined, and a taxonomy of main approaches to analysis is presented, including complete-case and available-case analysis, weighting, maximum likelihood, Bayes, single and multiple imputation, and augmented inverse probability weighting. Missingness mechanisms, which play a key role in the performance of alternative methods, are defined. Approaches to robust inference, and to inference when the mechanism is potentially missing not at random, are discussed.

本文回顾了临床心理学研究中处理缺失数据的方法。对缺失数据进行了定义,并对主要分析方法进行了分类,包括完整病例和可用病例分析、加权、最大似然法、贝叶斯法、单项和多项估算以及增强反概率加权法。定义了在替代方法的性能中起关键作用的缺失机制。此外,还讨论了稳健推断方法,以及当机制可能不是随机缺失时的推断方法。临床心理学年度评论》第 20 卷的最终在线出版日期预计为 2024 年 5 月。修订后的预计日期请参见 http://www.annualreviews.org/page/journal/pubdates。
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引用次数: 0
Epidemiology of Mass Shootings in the United States. 美国大规模枪击案的流行病学。
IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-07-01 Epub Date: 2024-07-02 DOI: 10.1146/annurev-clinpsy-081122-010256
Jillian K Peterson, James A Densley, Molly Hauf, Jack Moldenhauer

This in-depth review delves into the multifaceted realm of mass shootings and explores their epidemiology from a psychological perspective. The article presents a comprehensive examination of the prevalence, perpetrator and victim profiles, motives, and contributing factors associated with mass shootings. By investigating the intricate relationship between masculinity, domestic violence, military service, social media, fame-seeking, suicidal ideation, mental illness, and firearms, this article sheds light on the multifaceted nature of mass shootings. Moreover, it discusses the importance of implementing effective prevention strategies to address this growing public health concern. The findings from this review serve as a valuable resource for researchers, practitioners, policy makers, and the community at large, facilitating a deeper understanding of mass shootings and fostering the development of evidence-based solutions to prevent these tragic incidents.

这篇深度评论深入探讨了大规模枪击事件的多面性,并从心理学角度探讨了其流行病学。文章对大规模枪击案的发生率、犯罪者和受害者的特征、动机以及相关诱因进行了全面研究。通过研究男性气质、家庭暴力、兵役、社交媒体、追名逐利、自杀意念、精神疾病和枪支之间错综复杂的关系,本文揭示了大规模枪击事件的多面性。此外,文章还讨论了实施有效预防策略以解决这一日益严重的公共卫生问题的重要性。本综述的研究结果是研究人员、从业人员、政策制定者和整个社会的宝贵资源,有助于加深对大规模枪击事件的理解,并促进循证解决方案的发展,以防止这些悲剧事件的发生。临床心理学年度评论》第 20 卷的最终在线出版日期预计为 2024 年 5 月。修订后的预计日期请参见 http://www.annualreviews.org/page/journal/pubdates。
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引用次数: 0
Reading and Writing the Human Glycocode 读写人类糖码
IF 16.6 1区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-26 DOI: 10.1146/annurev-biochem-030122-044347
Noortje de Haan, Mathias I. Nielsen, Hans H. Wandall
The complex carbohydrate structures decorating human proteins and lipids, also called glycans, are abundantly present at cell surfaces and in the secretome. Glycosylation is vital for biological processes including cell–cell recognition, immune responses, and signaling pathways. Therefore, the structural and functional characterization of the human glycome is gaining more and more interest in basic biochemistry research and in the context of developing new therapies, diagnostic tools, and biotechnology applications. For glycomics to reach its full potential in these fields, it is critical to appreciate the specific factors defining the function of the human glycome. Here, we review the glycosyltransferases (the writers) that form the glycome and the glycan-binding proteins (the readers) with an essential role in decoding glycan functions. While abundantly present throughout different cells and tissues, the function of specific glycosylation features is highly dependent on their context. In this review, we highlight the relevance of studying the glycome in the context of specific carrier proteins, cell types, and subcellular locations. With this, we hope to contribute to a richer understanding of the glycome and a more systematic approach to identifying the roles of glycosylation in human physiology.
装饰人类蛋白质和脂质的复杂碳水化合物结构,也称为聚糖,大量存在于细胞表面和分泌组中。糖基化对细胞识别、免疫反应和信号通路等生物过程至关重要。因此,在基础生物化学研究以及开发新的疗法、诊断工具和生物技术应用的背景下,对人类糖粒的结构和功能进行表征正受到越来越多的关注。要使糖组学在这些领域充分发挥其潜力,关键是要了解定义人类糖组功能的特定因素。在这里,我们回顾了形成糖粒结构的糖基转移酶(作者)和在解码糖粒功能中起重要作用的糖结合蛋白(读者)。特定糖基化特征虽然大量存在于不同的细胞和组织中,但其功能在很大程度上取决于它们的环境。在这篇综述中,我们强调了在特定载体蛋白、细胞类型和亚细胞位置的背景下研究糖基结果的意义。我们希望借此加深对糖基化结果的理解,并采用更系统的方法来确定糖基化在人类生理学中的作用。
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引用次数: 0
Structure and Mechanisms of Assembly-Line Polyketide Synthases. 组装线多酮合成酶的结构和机制。
IF 16.6 1区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-25 DOI: 10.1146/annurev-biochem-080923-043654
Alexander M Soohoo, D. Cogan, Krystal L Brodsky, C. Khosla
Three decades of studies on the multifunctional 6-deoxyerythronolide B synthase have laid a foundation for understanding the chemistry and evolution of polyketide antibiotic biosynthesis by a large family of versatile enzymatic assembly lines. Recent progress in applying chemical and structural biology tools to this prototypical assembly-line polyketide synthase (PKS) and related systems has highlighted several features of their catalytic cycles and associated protein dynamics. There is compelling evidence that multiple mechanisms have evolved in this enzyme family to channel growing polyketide chains along uniquely defined sequences of 10-100 active sites, each of which is used only once in the overall catalytic cycle of an assembly-line PKS. Looking forward, one anticipates major advances in our understanding of the mechanisms by which the free energy of a repetitive Claisen-like reaction is harnessed to guide the growing polyketide chain along the assembly line in a manner that is kinetically robust yet evolutionarily adaptable.
三十年来对多功能 6-脱氧赤藓醇内酯 B 合成酶的研究,为了解多酮内酯抗生素生物合成的化学和进化奠定了基础。最近,在将化学和结构生物学工具应用于这种原型装配线聚酮内酯合成酶(PKS)和相关系统方面取得了进展,突显了其催化循环和相关蛋白质动态的一些特征。有令人信服的证据表明,在这个酶家族中已经演化出多种机制,可以沿着由 10-100 个活性位点组成的独特定义序列引导生长的多酮苷链,而每个活性位点在组装线式多酮苷合成酶的整个催化循环中只使用一次。展望未来,我们对重复性克来森类反应自由能的利用机制的理解有望取得重大进展,从而引导不断生长的多酮苷链沿着装配线以一种动力学上稳健、进化上适应性强的方式前进。
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引用次数: 0
Soluble Human Lectins at the Host–Microbe Interface 宿主-微生物界面上的可溶性人类凝集素
IF 16.6 1区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-19 DOI: 10.1146/annurev-biochem-062917-012322
Amanda L. Peiffer, A.E. Dugan, L.L. Kiessling
Human lectins are integral to maintaining microbial homeostasis on the skin, in the blood, and at mucosal barriers. These proteins can recognize microbial glycans and inform the host about its microbial status. In accordance with their roles, their production can vary with tissue type. They also can have unique structural and biochemical properties, and they can influence microbial colonization at sites proximal and distal to their tissue of origin. In line with their classification as innate immune proteins, soluble lectins have long been studied in the context of acute infectious disease, but only recently have we begun to appreciate their roles in maintaining commensal microbial communities (i.e., the human microbiota). This review provides an overview of soluble lectins that operate at host–microbe interfaces, their glycan recognition properties, and their roles in physiological and pathological mechanisms.
人类凝集素是维持皮肤、血液和粘膜屏障中微生物平衡不可或缺的物质。这些蛋白质可以识别微生物糖类,并告知宿主其微生物状态。根据其作用,它们的产量会随组织类型而变化。它们还可能具有独特的结构和生化特性,并能影响微生物在其起源组织近端和远端的定植。可溶性凝集素被归类为先天性免疫蛋白,因此长期以来一直被用于研究急性传染病,但直到最近我们才开始认识到它们在维持共生微生物群落(即人类微生物群)中的作用。本综述概述了在宿主-微生物界面上发挥作用的可溶性凝集素、它们的聚糖识别特性及其在生理和病理机制中的作用。
{"title":"Soluble Human Lectins at the Host–Microbe Interface","authors":"Amanda L. Peiffer, A.E. Dugan, L.L. Kiessling","doi":"10.1146/annurev-biochem-062917-012322","DOIUrl":"https://doi.org/10.1146/annurev-biochem-062917-012322","url":null,"abstract":"Human lectins are integral to maintaining microbial homeostasis on the skin, in the blood, and at mucosal barriers. These proteins can recognize microbial glycans and inform the host about its microbial status. In accordance with their roles, their production can vary with tissue type. They also can have unique structural and biochemical properties, and they can influence microbial colonization at sites proximal and distal to their tissue of origin. In line with their classification as innate immune proteins, soluble lectins have long been studied in the context of acute infectious disease, but only recently have we begun to appreciate their roles in maintaining commensal microbial communities (i.e., the human microbiota). This review provides an overview of soluble lectins that operate at host–microbe interfaces, their glycan recognition properties, and their roles in physiological and pathological mechanisms.","PeriodicalId":7980,"journal":{"name":"Annual review of biochemistry","volume":null,"pages":null},"PeriodicalIF":16.6,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140626315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Triumphs and Challenges of Natural Product Discovery in the Postgenomic Era. 后基因组时代天然产物发现的胜利与挑战》。
IF 16.6 1区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-19 DOI: 10.1146/annurev-biochem-032620-104731
Carolina Cano-Prieto, Agustina Undabarrena, Ana Calheiros de Carvalho, Jay D Keasling, Pablo Cruz-Morales
Natural products have played significant roles as medicine and food throughout human history. Here, we first provide a brief historical overview of natural products, their classification, biosynthetic origins, and the microbiological and genetic methods used for their discovery. We also describe and discuss the technologies that revolutionized the field, which transitioned from classic genetics to genome-centric discovery approximately two decades ago. We then highlight the most recent advancements and approaches in the current postgenomic era, in which genome mining is a standard operation and high-throughput analytical methods allow parallel discovery of genes and molecules at an unprecedented pace. Finally, we discuss the new challenges faced by the field of natural products and the future of systematic heterologous expression and strain-independent discovery, which promises to deliver more molecules in vials than ever before.
在人类历史上,天然产物作为药物和食品发挥了重要作用。在此,我们首先简要介绍了天然产物的历史、分类、生物合成起源以及发现天然产物所使用的微生物学和遗传学方法。我们还描述并讨论了该领域的革命性技术,大约二十年前,该领域从传统的遗传学过渡到以基因组为中心的发现。然后,我们重点介绍了当前后基因组时代的最新进展和方法,在这个时代,基因组挖掘是一项标准操作,高通量分析方法允许以前所未有的速度并行发现基因和分子。最后,我们讨论了天然产物领域面临的新挑战,以及系统异源表达和菌株独立发现的未来,这有望在小瓶中提供比以往更多的分子。
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引用次数: 0
ATP-Dependent Steps in Peroxisomal Protein Import 过氧化物酶体蛋白质导入的 ATP 依赖性步骤
IF 16.6 1区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-15 DOI: 10.1146/annurev-biochem-030222-111227
Harald W. Platta, Julia Jeske, Nadine Schmidt, Ralf Erdmann
Peroxisomes are organelles that play a central role in lipid metabolism and cellular redox homeostasis. The import of peroxisomal matrix proteins by peroxisomal targeting signal (PTS) receptors is an ATP-dependent mechanism. However, the energy-dependent steps do not occur early during the binding of the receptor–cargo complex to the membrane but late, because they are linked to the peroxisomal export complex for the release of the unloaded receptor. The first ATP-demanding step is the cysteine-dependent monoubiquitination of the PTS receptors, which is required for recognition by the AAA+ peroxins. They execute the second ATP-dependent step by extracting the ubiqitinated PTS receptors from the membrane for release back to the cytosol. After deubiquitination, the PTS receptors regain import competence and can facilitate further rounds of cargo import. Here, we give a general overview and discuss recent data regarding the ATP-dependent steps in peroxisome protein import.
过氧物酶体是一种细胞器,在脂质代谢和细胞氧化还原平衡中发挥着核心作用。过氧化物酶体靶向信号(PTS)受体导入过氧化物酶体基质蛋白是一种 ATP 依赖性机制。然而,能量依赖性步骤不是在受体-货物复合体与膜结合的早期发生,而是在后期发生,因为它们与过氧化物酶体导出复合体相关联,以释放卸载的受体。第一个需要 ATP 的步骤是 PTS 受体的半胱氨酸依赖性单泛素化,这是 AAA+ 过氧化物酶识别所必需的。它们执行的第二个 ATP 依赖性步骤是将泛素化的 PTS 受体从膜中提取出来,释放回细胞质。去泛素化后,PTS 受体恢复了导入能力,并能促进下一轮的货物导入。在此,我们将对过氧化物酶体蛋白质导入过程中的 ATP 依赖性步骤进行概述并讨论最新数据。
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引用次数: 0
Leucine-Rich Repeat Kinases 富亮氨酸重复激酶
IF 16.6 1区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-15 DOI: 10.1146/annurev-biochem-030122-051144
Dario R. Alessi, Suzanne R. Pfeffer
Activating mutations in leucine-rich repeat kinase 2 (LRRK2) represent the most common cause of monogenic Parkinson's disease. LRRK2 is a large multidomain protein kinase that phosphorylates a specific subset of the ∼65 human Rab GTPases, which are master regulators of the secretory and endocytic pathways. After phosphorylation by LRRK2, Rabs lose the capacity to bind cognate effector proteins and guanine nucleotide exchange factors. Moreover, the phosphorylated Rabs cannot interact with their cognate prenyl-binding retrieval proteins (also known as guanine nucleotide dissociation inhibitors) and, thus, they become trapped on membrane surfaces. Instead, they gain the capacity to bind phospho-Rab-specific effector proteins, such as RILPL1, with resulting pathological consequences. Rab proteins also act upstream of LRRK2 by controlling its activation and recruitment onto membranes. LRRK2 signaling is counteracted by the phosphoprotein phosphatase PPM1H, which selectively dephosphorylates phospho-Rab proteins. We present here our current understanding of the structure, biochemical properties, and cell biology of LRRK2 and its related paralog LRRK1 and discuss how this information guides the generation of LRRK2 inhibitors for the potential benefit of patients.
富亮氨酸重复激酶 2(LRRK2)的激活突变是单基因帕金森病最常见的病因。LRRK2 是一种大型多域蛋白激酶,可将人类 Rab GTPases 的 65 个特定亚群磷酸化,而 Rab GTPases 是分泌和内分泌途径的主要调节因子。经 LRRK2 磷酸化后,Rabs 失去了结合同源效应蛋白和鸟嘌呤核苷酸交换因子的能力。此外,磷酸化的 Rabs 无法与其同源的前酰结合检索蛋白(也称为鸟嘌呤核苷酸解离抑制剂)相互作用,因此会被困在膜表面。相反,它们会获得结合磷酸化 Rab 特异性效应蛋白(如 RILPL1)的能力,从而导致病理后果。Rab 蛋白还通过控制 LRRK2 的激活和在膜上的招募,在其上游发挥作用。LRRK2 信号被磷蛋白磷酸酶 PPM1H 抵消,PPM1H 选择性地使磷酸化 Rab 蛋白去磷酸化。我们在此介绍我们目前对 LRRK2 及其相关旁系亲属 LRRK1 的结构、生化特性和细胞生物学的理解,并讨论这些信息如何指导 LRRK2 抑制剂的研发,从而为患者带来潜在的益处。
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引用次数: 0
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Annual review of biochemistry
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