Pub Date : 2015-03-20DOI: 10.5272/JIMAB.2015211.732
P. Kosev, Cvetan Sokolov, Y. Andonov, B. Valentinov
We present our experience with the MIS TKA through a mid-vastus approach. The indications and contraindications of the technique are discussed. Our series include 104 patients. The Knee Society Score is used to rate the results. The average KSS score on 6-th month is 89 points. Eleven patients had skin necrosis. Eight had transient stiffness. One developed late infection. The MIS-TKA is recommended for selected patients and must be performed by an experienced surgeon and prepared team.
{"title":"Minimally invasive total knee arthroplasty.","authors":"P. Kosev, Cvetan Sokolov, Y. Andonov, B. Valentinov","doi":"10.5272/JIMAB.2015211.732","DOIUrl":"https://doi.org/10.5272/JIMAB.2015211.732","url":null,"abstract":"We present our experience with the MIS TKA through a mid-vastus approach. The indications and contraindications of the technique are discussed. Our series include 104 patients. The Knee Society Score is used to rate the results. The average KSS score on 6-th month is 89 points. Eleven patients had skin necrosis. Eight had transient stiffness. One developed late infection. The MIS-TKA is recommended for selected patients and must be performed by an experienced surgeon and prepared team.","PeriodicalId":79832,"journal":{"name":"Clinical privilege white paper","volume":"44 1","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2015-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84767512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1002/9781119548935.ch56
S. Hall
{"title":"Surgical first assistant.","authors":"S. Hall","doi":"10.1002/9781119548935.ch56","DOIUrl":"https://doi.org/10.1002/9781119548935.ch56","url":null,"abstract":"","PeriodicalId":79832,"journal":{"name":"Clinical privilege white paper","volume":"23 1","pages":"1-21"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78174013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1097/00000446-199708000-00003
R. A. KtJNKLE, J. A. Riciit
Serapie is a naturally occurring fatal neurodegencrative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a recent study. it was shown that sheep intracercbrally inoculated with a US scrapie agent (No. 13-7) developed scrapie and survived for an average of 19 months post inoculation. In the present study, when this serapie inoculum was further passaged for 3 successive generations, the survival time was reduced by approximately 8 months in scrapie-susceptible (QQ on prion protein gene [PRNP] at codon 171) Suffolk sheep. It is concluded that inoculum No. 13-7 appears to have been stabilized in susceptible (171 QQ) Suffolk sheep and may be considered a specific isolate of sheep serapic agent in the USA and therefore that it can he used to evaluate other isolates of sheep scrapie in this country. Key n'ords: I rnmunohistochemistry PrP genetic susceptibility, sheep scrapic strains transmissible spongilorm encephalopathies (TSEs): Western blot. Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. The susceptibility of' sheep to scrapie is dependent on genetic variation of the host prion protein (PRNP) gene) 410 PRNP genotypes are defined by variations in the amino acids located at codons 136, 154, and 171. At least 5 variant alleles have been found, and they are depicted as ARQ, ARR, VRQ, AHQ, and ARH. The codes represent amino acids at codons 136, 154. and 171, that is, A-136, R-154. and Q-171 (ARQ). where A = alanine, R = arginine. Q = glutamine. H = histidine. and V valine. The level of risk to contract scrapie varies depending on breed and the genotypes found within the fioek ARR are the most resistant, and VRQ are the most Susceptible. 1-4. A study of Suffolk sheep in the United States found 61 of orally inoculated animals developed scrapie. ' All were homozygous for glutanline (QQ) at allele 171 on PRNP gene* In a recent study. it was shown that sheep intracerebrally inoculated with US scrapie agent (inoculum No. 13-7) developed disease within an average of 19 months post inoculation (MPI)) This inoculurn was made with brain tissue of 13 sheep from 7 different source flocks)' This study documents incubation times, pathologic findings, and distribution of abnormal prion Present address: Department of Diagnostic Medicine and Pathobiology. Kansas State University, Manhattan. KS. protein (p1.pSe) by immunohistochemical (IHC) and Western blot (WB) techniques in tissues of genetically susceptible Suffolk sheep (QQ or QH at codon 171 of PRNP gene) that were intracerebrally inoculated with 3 consecutive passages of inoculum No. 13-7 (Table I). The objectives of the study were to reduce the incubation (post inoculation) time of the scrapie inoculum and to stabilize the inoculum so that it can be used to evaluate other isolates of sheep scrapie in this country. Materials and Methods A total of 18 Suffolk lambs (females and castrated males) from National An
塞拉皮病是绵羊和山羊的一种自然发生的致命神经退行性疾病。对这种疾病的易感性部分取决于宿主的基因组成。在最近的研究中。结果表明，在羊脑内接种一种美国痒病剂(13-7号)可发生痒病，接种后平均存活19个月。在本研究中，当该疫苗进一步传代3代时，在痒病易感(密码子171上朊蛋白基因[PRNP]上的QQ)萨福克羊的存活时间减少了约8个月。结果表明，13-7号接种物在易感的(171 QQ)萨福克羊中表现稳定，可作为美国羊痒病剂的特异性分离物，可用于评价美国其他羊痒病分离物。关键词:免疫组织化学PrP遗传易感性羊刮伤株传染性海绵状脑病(tse) Western blot痒病是绵羊和山羊的一种自然发生的致命神经退行性疾病。绵羊对痒病的易感性依赖于宿主朊病毒蛋白(PRNP)基因的遗传变异。PRNP基因型由位于密码子136、154和171的氨基酸变异来定义。已发现至少5个变异等位基因，分别为ARQ、ARR、VRQ、AHQ和ARH。这些密码子代表密码子136,154上的氨基酸。171，即A-136, R-154。Q-171 (ARQ)。其中A =丙氨酸，R =精氨酸。Q =谷氨酰胺。H =组氨酸。V和缬氨酸。感染痒病的风险水平因品种而异，在fioek ARR中发现的基因型最具抗性，而VRQ最易感。一项对美国萨福克羊的研究发现，61只口服疫苗的羊患上了痒病。在PRNP基因171等位基因上均为谷氨酸(QQ)纯合*。结果表明，接种美国痒病剂(接种编号13-7)的绵羊在接种后平均19个月内发病(MPI)。该接种物由7个不同来源群的13只羊的脑组织制成。本研究记录了异常朊病毒的潜伏期、病理表现和分布。堪萨斯州立大学，曼哈顿。KS。蛋白质(p1.pSe)免疫组织化学(包含IHC)和免疫印迹(WB)技术的组织遗传易感萨福克羊(QQ或QH PRNP基因的密码子171),鼠脑内接种连续3通道的培养液13号(表1)。这项研究的目标是降低孵化(post接种)时间的痒病剂和稳定剂,它可以用来评估羊痒病在这个国家的其他隔离。材料与方法从美国国家动物疾病中心(National Animal Disease Center, NADC)的无痒病羊群中选取18只萨福克羔羊(雌性和阉割的雄性)，在约4月龄时接种痒病疫苗。除1只羊(编号9102，表1)外，其余羊在密码子136、154、171处均为AAIRR/QQ。分别(表1)。痒病疫苗(13-7号)通过脑内接种在4代羔羊(1999年至2004年)中传代(表1)。最初，该疫苗是从7个源群的13只患痒病的羊脑(经免疫组化技术检测均为阳性)中制备的。疫苗在机械研磨机中研磨。以IOU pg/mI的速度加入庆大霉素，用磷酸盐缓冲的红生理盐水制成最终浓度i0 (wt/vol)。在随后的传代中，从潜伏期最短的动物身上获得了被瘙痒病感染的脑组织
{"title":"Infectious disease.","authors":"R. A. KtJNKLE, J. A. Riciit","doi":"10.1097/00000446-199708000-00003","DOIUrl":"https://doi.org/10.1097/00000446-199708000-00003","url":null,"abstract":"Serapie is a naturally occurring fatal neurodegencrative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a recent study. it was shown that sheep intracercbrally inoculated with a US scrapie agent (No. 13-7) developed scrapie and survived for an average of 19 months post inoculation. In the present study, when this serapie inoculum was further passaged for 3 successive generations, the survival time was reduced by approximately 8 months in scrapie-susceptible (QQ on prion protein gene [PRNP] at codon 171) Suffolk sheep. It is concluded that inoculum No. 13-7 appears to have been stabilized in susceptible (171 QQ) Suffolk sheep and may be considered a specific isolate of sheep serapic agent in the USA and therefore that it can he used to evaluate other isolates of sheep scrapie in this country. Key n'ords: I rnmunohistochemistry PrP genetic susceptibility, sheep scrapic strains transmissible spongilorm encephalopathies (TSEs): Western blot. Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. The susceptibility of' sheep to scrapie is dependent on genetic variation of the host prion protein (PRNP) gene) 410 PRNP genotypes are defined by variations in the amino acids located at codons 136, 154, and 171. At least 5 variant alleles have been found, and they are depicted as ARQ, ARR, VRQ, AHQ, and ARH. The codes represent amino acids at codons 136, 154. and 171, that is, A-136, R-154. and Q-171 (ARQ). where A = alanine, R = arginine. Q = glutamine. H = histidine. and V valine. The level of risk to contract scrapie varies depending on breed and the genotypes found within the fioek ARR are the most resistant, and VRQ are the most Susceptible. 1-4. A study of Suffolk sheep in the United States found 61 of orally inoculated animals developed scrapie. ' All were homozygous for glutanline (QQ) at allele 171 on PRNP gene* In a recent study. it was shown that sheep intracerebrally inoculated with US scrapie agent (inoculum No. 13-7) developed disease within an average of 19 months post inoculation (MPI)) This inoculurn was made with brain tissue of 13 sheep from 7 different source flocks)' This study documents incubation times, pathologic findings, and distribution of abnormal prion Present address: Department of Diagnostic Medicine and Pathobiology. Kansas State University, Manhattan. KS. protein (p1.pSe) by immunohistochemical (IHC) and Western blot (WB) techniques in tissues of genetically susceptible Suffolk sheep (QQ or QH at codon 171 of PRNP gene) that were intracerebrally inoculated with 3 consecutive passages of inoculum No. 13-7 (Table I). The objectives of the study were to reduce the incubation (post inoculation) time of the scrapie inoculum and to stabilize the inoculum so that it can be used to evaluate other isolates of sheep scrapie in this country. Materials and Methods A total of 18 Suffolk lambs (females and castrated males) from National An","PeriodicalId":79832,"journal":{"name":"Clinical privilege white paper","volume":"19 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85900651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physician assistants in the emergency department.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79832,"journal":{"name":"Clinical privilege white paper","volume":" 409","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33232464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Allergy and immunology.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79832,"journal":{"name":"Clinical privilege white paper","volume":" 124","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33232461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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