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Effect of cryopreservation of individual ejaculates on fertility in genetic resource chicken lines 个体射精低温保存对遗传资源鸡系育性的影响
Pub Date : 2017-05-16 DOI: 10.1399/eps.2017.185
C. Ehling, U. Baulain, S. Weigend, M. Henning, D. Rath
The aim of this study was to confirm the suitability of a cryopreservation protocol in three phylogenetically divergent chicken lines on individual ejaculates of roosters. The experimental animals originated from three gene reserve lines. In two trials individual semen samples were cryopreserved and used in insemination experiments. For the freezing medium N-methylacetamide and dimethylformamide were mixed 2:1 in a base diluent according to Hanzawa et al. ( 2006). 0.25 ml straws were frozen in two phases beginning with a controlled temperature decrease of –3°C/min. The samples were thawed in a 4°C water bath. The semen was evaluated by means of CASA (computer-assisted sperm analysis). The hens were intravaginal inseminated in the distance of three or four days three times. Fertilization rate was determined after 7-day incubation by candling. Results showed a considerable influence of trial, chicken line and their interaction on sperm quality and fertility. Fertilization results with frozen sperm were extremely variable between individual roosters within the lines and trials (line R22: 9.7–73.3%, line G11: 33.3–84.6%, line L68: 41.4–87.5%). Significant correlations between sperm quality parameters and fertility rates were found only in two of three chicken lines. The results prove that there are genetic differences in the sperm quality and the subsequent fertility which should be taken into account at the construction of a sperm bank. But the developed cryopreservation method was applied successfully to individual roosters of genetic resource lines and is therefore eligible for gene banking of endangered chicken populations.
本研究的目的是确认在三个系统发育不同的鸡系中对公鸡个体射精的低温保存方案的适用性。实验动物来源于三个基因储备系。在两个试验中,个体精液样本被冷冻保存并用于人工授精实验。根据Hanzawa et al.(2006)的方法,将n -甲基乙酰胺和二甲基甲酰胺在基础稀释剂中以2:1混合作为冷冻介质。0.25 ml吸管分两期冷冻,开始时控制温度降低-3°C/min。样品在4°C水浴中解冻。采用计算机辅助精子分析(CASA)对精液进行评估。这些母鸡在三到四天的距离内进行了三次阴道内受精。熏蒸7 d后测定受精率。结果表明,试验、鸡系及其互作对精子质量和育性有较大影响。用冷冻精子受精的结果在系内和试验中的单个公鸡之间差异极大(系R22: 9.7-73.3%,系G11: 33.3-84.6%,系L68: 41.4-87.5%)。精子质量参数与生育率之间的显著相关性仅在三个鸡系中的两个中被发现。结果证明,在精子质量和随后的生育能力方面存在遗传差异,这在建立精子库时应予以考虑。但所开发的冷冻保存方法已成功地应用于遗传资源系的公鸡个体,因此符合濒危鸡种群基因库的条件。
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引用次数: 3
Combined effect of short-term temperature stimulation during the hatching phase and of protein and energy concentrations in the diets on performance of laying-type cockerels of different strains 孵化期短期温度刺激及饲粮蛋白质和能量浓度对不同品系产蛋型公鸡生产性能的综合影响
Pub Date : 2017-02-15 DOI: 10.1399/eps.2017.174
I. Halle, B. Tzschentke
The following described study was carried out to investigate the hypothesis that short-term warm stimulation in incubation temperature during the last days of incubation can improve hatching results and have long-lasting effect on performance, also in laying-type cockerels. The results of the incubation trial showed that a short-term increase in temperature by 1°C over the standard for 2 hours daily (38.2–38.4°C) improved the hatching results by 3.5% and in female and 7.7% in male LB chickens and by 2.0% in female and 5.5% in male LT chickens. The statistical evaluation of the feed level as single effect on performance of cockerels demonstrated a high dependence (P < 0.05) of the protein and energy concentration in the feed on the daily feed intake, the body weight, and the feed to gain ratio. The feed to gain ratio was significantly higher in the low feed groups (2.59 kg/kg) compared to the high feed groups (2.33 kg/kg) over the 70 days growing period. The origin of the cockerels was of great importance on the feed intake (LB-45.7 g/d/bird; LT-47.0 g/d/bird) over the growing phase D22–50 and on body weight at D21 (LT-256 g; LB-242 g) and D70 (LT-1404 g; LB-1380 g). The feed to gain ratio of the LT-cockerels (LT-2.24 kg/kg) was significantly higher compared to the LB- birds (LB-2.16 kg/kg) at the age of D22 to D49, but in the next growing period (D50–70) the order changed and over the total growing period (D1–70) there was no difference between the two origins of cockerels. Over the total growing period (D1–70), the combined effect of incubation temperature+origin of cockerels demonstrated dependence (P <0.05) of both parameters on the daily feed intake and daily weight gain, as well as on the final body weight. A significant interaction (P <0.05) between the origin of cockerels+feed level on daily feed intake (D1-D70) underlined the importance of both these factors on growing parameters. At slaughtering at D49 the percentage of carcass and mass of two legs were statistically different between LT (62.8%; 164.8 g) and LB (61.0%; 155.8 g) cockerels. At the end of the trial (D70) the mass and percentages of meat and organs were not different between LB and LT birds. A firm statistical difference in the percentage of liver and gizzard was calculated between low and high feed level at the age of D49 (gizzard – 2.8%, 3.1%) and D70 (gizzard – 2.6%, 2.8%). The combined effect of incubation temperature+origin of cockerels demonstrates dependence (P < 0.05) of both parameters on yield and percentage of breast meat over the total growing period (D1–70).
本研究旨在探讨在孵育最后几天对孵育温度进行短期温暖刺激可以改善孵化结果并对产蛋型公鸡的生产性能产生长期影响的假设。孵育试验结果表明,每天(38.2 ~ 38.4℃)在标准温度基础上短期升高1℃,使LB鸡的孵化率提高3.5%,雄LB鸡提高7.7%;LT鸡的孵化率提高2.0%,雄LB鸡的孵化率提高5.5%。饲料水平对公鸡生产性能的单因素影响统计评价表明,饲料中蛋白质和能量浓度与日采食量、体重和料重比高度依赖(P < 0.05)。70 d生长期,低饲粮组料重比(2.59 kg/kg)显著高于高饲粮组(2.33 kg/kg)。雏鸡的来源对采食量有重要影响(LB-45.7 g/d/只;在第22 - 50生长期(LT-47.0 g/d/只)和第21期(LT-256 g;LB-242 g)和D70 (LT-1404 g;在D22 ~ D49时,lt -鸡的料重比(LT-2.24 kg/kg)显著高于LB-鸡(LB-2.16 kg/kg),但在接下来的生长期(d50 ~ d70),这一顺序发生了变化,在整个生长期(d1 ~ 70),两种来源的公鸡之间没有差异。在整个生长期(d1 ~ 70),孵育温度+产地对雏鸡日采食量、日增重及最终体重均有显著的依赖性(P <0.05)。雏鸡产地与饲料水平对日采食量(d1 ~ d70)的交互作用显著(P <0.05),说明这两个因素对生长参数的重要性。在D49屠宰时,两组间的屠宰率和两腿质量有统计学差异(62.8%;164.8 g)和LB (61.0%;155.8 g)小公鸡。试验结束(D70)时,LB和LT鸡的肉和脏器质量和百分比无显著差异。计算了低饲粮水平和高饲粮水平对49日龄(砂囊- 2.8%,3.1%)和70日龄(砂囊- 2.6%,2.8%)肝脏和砂囊百分比的显著统计学差异。孵育温度+雏鸡产地对整个生长期(d1 ~ 70)产蛋率和胸肉率的影响均呈相关性(P < 0.05)。
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引用次数: 1
Comparative analyses of layer males, dual purpose males and mixed sex broilers kept for fattening purposes regarding their floor space covering, weight-gain and several animal health traits 育肥用蛋鸡、两用肉鸡和混合性肉鸡占地面积、增重和若干动物健康性状的比较分析
Pub Date : 2016-04-19 DOI: 10.1399/EPS.2016.128
C. Habig, M. Beyerbach, N. Kemper
As a major part of world poultry production is specialised in meat or egg production, millions of day-old male layer chicks are culled in Europe each year. Within the present study, about 500 birds each of three genetic lines, Lohmann Brown (LB), Lohmann Dual (LD) and Ross 308 were fattened separately in a poultry floor management system over an eleven-week period. At regular intervals 55 birds per line were weighed and the floor space covered by their bodies was calculated using planimetric measurements. Furthermore, several animal health traits were assessed within the final two weeks of fattening. The mean floor area occupied by one-week old Ross 308 broilers was approximately 1.5‑fold higher compared to those of LB and LD at the same age and extended up to more than twice the space with increasing age. The highest mean body weight was measured for Ross 308, followed by LD and LB. While uniformity of the LB flock was high at any time body weights of LD and Ross 308 encompassed a wide range, resulting in a low uniformity within these groups. None of the examined LB and LD chickens showed keel bone deformities, breast plumage pollution or foot pad dermatitis. In contrast, all of the Ross 308 broilers had moderate to severe pollution of breast plumage and up to 27% showed lesions on at least one foot pad at the end of the fattening period. About 91% of the LD and 56% of the LB chickens showed lesions at the comb and/or wattle at the end of the fattening period, indicating aggressive male-male interactions within these groups. The mean eviscerated carcass weight was almost four times higher for Ross 308 (4.11 kg) than for LB (1.05 kg) and LD (1.03 kg).
由于世界家禽生产的主要部分是专门从事肉类或蛋类生产,欧洲每年有数百万只日龄的蛋鸡被扑杀。在本研究中,在11周的时间里,罗曼布朗(LB)、罗曼双(LD)和罗斯308 (Ross 308)三个遗传系各约500只鸡在家禽地板管理系统中分别育肥。每隔一段时间,对每行55只鸟进行称重,并使用平面测量法计算它们身体所覆盖的地板面积。此外,在育肥的最后两周内评估了几种动物的健康特征。1周龄罗斯308肉鸡的平均占地面积是同龄LB和LD肉鸡的1.5倍左右,随着年龄的增长,占地面积增加了一倍以上。Ross 308的平均体重最高,其次是LD和LB。而LB群的均匀性在任何时候都很高,LD和Ross 308的体重范围很广,导致各组之间的均匀性较低。检查的LB和LD鸡均未出现龙骨畸形、胸毛污染或足底皮炎。相比之下,所有罗斯308肉鸡的胸毛都有中度至重度污染,27%的肉鸡在育肥期结束时至少有一个脚垫出现损伤。育肥期结束时,约91%的LD鸡和56%的LB鸡鸡冠和/或鸡冠处出现病变,表明这两组鸡之间存在攻击性的雄性相互作用。罗斯308的平均净膛胴体重(4.11 kg)几乎是LB (1.05 kg)和LD (1.03 kg)的4倍。
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引用次数: 11
Clostridium perfringens challenge and dietary fat type modifies performance, microbiota composition and histomorphology of the broiler chicken gastrointestinal tract 产气荚膜梭菌攻毒和饲粮脂肪类型改变肉鸡胃肠道生产性能、微生物群组成和组织形态
Pub Date : 2016-01-01 DOI: 10.1399/EPS.2016.130
D. Josefiak, S. Świątkiewicz, B. Kierończyk, M. Rawski, J. Długosz, R. Engberg, O. Højberg
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引用次数: 8
Phylogenic versus selection effects on growth development, egg laying and egg quality in purebred laying hens 系统发育与选择对纯种蛋鸡生长发育、产蛋和蛋品质的影响
Pub Date : 2015-05-26 DOI: 10.1399/EPS.2015.89
M. Lieboldt, I. Halle, J. Frahm, L. Schrader, U. Baulain, M. Henning, R. Preisinger, S. Dänicke, S. Weigend
The aim of the present study was to assess the performance traits of chicken lines with different performance level and phylogenetic origin. Selection for high performances may change unselected traits related to animal health and well-being. However, long before intense selection started to act leading to contemporary high performing genotypes, founder populations on egg laying breeds with white and brown egg shell had been separated for many generations and have evolved independently. We have started to set up a comprehensive collaborative effort at the Friedrich-Loeffler-Institut to approach research question related to the capacity of high selected chicken lines to cope with limited metabolic resources. As a first step, four genotypes of purebred laying hens (WLA, BLA, R11 and L68) were used, which were divided by their divergence in performance and phylogeny. For the first time these genotypes were characterized according to their performance and growth development in the first 16 weeks of age in a rearing trial, a pre-laying period of 6 weeks and a following performance trial of 13 laying months (23 rd to 74 th week of age). The investigated performance traits were significantly affected by genotype, age and their interaction (p ≤ 0.001). As a result of selection for high laying performance, selected strains showed a significantly higher performance than the non-selected ones. The high performing genotypes had an average laying intensity of 85 to 90%, a daily egg mass production of approximately 50 g/hen/d and a feed to egg mass ratio of 2.1 to 2.3 kg/kg. However, the low performing genotypes had an average laying intensity of 52 to 56%, a daily egg mass production of approximately 26 to 31 g/hen/d and a feed to egg mass ratio of approximately 3.0 kg/kg. Concerning average egg weight only R11 (50 g/egg) differed from the other experimental lines (55 to 58 g/egg). Independently of their performance brown hens showed a significantly higher body weight than white hens during the whole trial. Egg quality analyses showed that high performing lines had a significantly higher albumen proportion (57.1 to 62.4%) and a significantly lower yolk proportion (26.8 to 29.8%) than the low performing lines (albumen: 55.3 to 57.4%, yolk: 30.3 to 33.5%). White hens (10.8 to 13.6%) had significantly higher proportion of egg shells than brown hens (10.3 to 12.9%). In summary, the studied genotypes showed clear differences in performance level that made them well suitable for the established experimental design. With that design further studies should be carried out under varying environmental conditions (e.g. feeding, housing, infectious diseases). Thereby the studies will examine the question whether selection on high performance (WLA, BLA) leads to a reduced adaptability to varying environmental conditions.
本研究旨在评价不同生产性能水平和系统发育来源的鸡系的生产性能性状。对高性能的选择可能会改变与动物健康和福祉相关的未选择性状。然而,早在激烈的选择开始导致当代高性能基因型之前,白色和棕色蛋壳产蛋品种的创始种群已经分离了许多代,并独立进化。我们已经开始在弗里德里希-吕弗勒研究所建立一个全面的合作努力,以解决与高选择鸡系应对有限代谢资源的能力有关的研究问题。首先选用4种基因型的纯种蛋鸡(WLA、BLA、R11和L68),根据其生产性能和系统发育差异进行分类。在饲养试验、产蛋前6周和产蛋后13个产蛋月(第23 ~ 74周龄)的性能试验中,首次对这些基因型进行了性能和生长发育鉴定。基因型、年龄及其交互作用对生产性状有显著影响(p≤0.001)。通过高产蛋性能的选择,选择品系的产蛋性能显著高于未选择品系。高产基因型平均产蛋强度为85% ~ 90%,日产蛋量约为50 g/只/d,料蛋比为2.1 ~ 2.3 kg/kg。而表现较差的基因型平均产蛋强度为52% ~ 56%,日产蛋量约为26 ~ 31 g/只/d,料蛋比约为3.0 kg/kg。在平均蛋重方面,只有R11 (50 g/蛋)与其他试验品系(55 ~ 58 g/蛋)存在差异。不考虑生产性能,整个试验期间,褐母鸡的体重显著高于白母鸡。蛋品质分析表明,高产品系的蛋白比例(57.1 ~ 62.4%)显著高于低产品系(蛋白比例55.3 ~ 57.4%,蛋黄比例30.3 ~ 33.5%),蛋黄比例(26.8 ~ 29.8%)显著低于低产品系(蛋白比例55.3 ~ 57.4%,蛋黄比例30.3 ~ 33.5%)。白母鸡的蛋壳比例(10.8% ~ 13.6%)显著高于褐母鸡(10.3 ~ 12.9%)。综上所述,所研究的基因型表现出明显的生产性能水平差异,使它们非常适合所建立的试验设计。根据这种设计,应在不同的环境条件下(例如喂养、住房、传染病)进行进一步的研究。因此,本研究将探讨对高性能(WLA, BLA)的选择是否会导致对不同环境条件的适应性降低。
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引用次数: 36
The phenotypic interrelationships between feather pecking, being feather pecked, feather eating, feather score, fear, body weight, and egg production traits in a F2- cross of White Leghorn lines selected for high and low severe feather pecking 高、低严重啄羽系F2杂交中啄羽、被啄羽、食羽、羽毛评分、恐惧、体重和产蛋量性状的表型相互关系
Pub Date : 2015-03-30 DOI: 10.1399/EPS.2015.84
S. Bögelein, J. Kjaer, J. Bennewitz, W. Bessei
It is generally assumed that there is a relationship between feather pecking, fear, feather condition and laying performance criteria in laying hens. This hypothesis was tested in a F2-cross of high (HFP) and low (LFP) feather pecking birds of White Leghorn origin. A total of 967 birds were first observed for the number of bouts of severe feather pecks delivered (FPD) and received (FPR) when kept in groups of random composition. The whole population was split for FPD in HFP and LFP using the threshold of ≥ 2 (HFP) and < 2 (LFP). The birds clearly differed in FPD but there was no significant difference for FPR between HFP and LFP. The subgroups were subjected to a pencil test at 29 weeks of age. At 26 weeks and 39 weeks of age body weight was measured and feather condition scored in different body parts (neck, breast, wings, vent and tail) using a scale from 0–4 (0 = worse, 4 = best). In addition the birds were tested for their feather eating behaviour and their laying performance criteria (hen-day egg production %, egg weight, feed consumption per day and FCR) at 29 weeks of age. In contrast to our expectation HFP birds showed a significant better plumage condition at 39 weeks of age than LFP birds. There were differences in feather scores at both ages for wings and tail. There were no differences between subgroups in the body weight at neither age. There were also no differences in the pencil test. But HFP birds showed a significant higher number of eaten feathers than LFP birds. This is in line with many other studies. But the difference was not as large as reported in previous studies. In contrast to our expectation there were no significant differences between the subgroups in performance, feed intake and FCR although LFP birds showed a significant poorer feather condition than HFP birds at 39 weeks of age. There were, however, negative correlations between feather score, performance, feed consumption and FCR within subgroups.Obviously there is no consistent relationships between feather pecking, fear, feather condition and performance criteria.
一般认为,蛋鸡啄羽毛、恐惧、羽毛状况与产蛋性能标准之间存在一定的关系。这一假设在高(HFP)和低(LFP)羽毛啄鸟的f2杂交中得到了检验。首先观察了967只鸟在随机分组时的严重啄羽次数(FPD)和接受(FPR)。采用HFP≥2 (HFP)和LFP < 2 (LFP)的阈值对整个人群进行FPD划分。禽的FPD有明显差异,但HFP和LFP之间FPR无显著差异。这些小组在29周龄时进行了铅笔测试。在26周龄和39周龄时测量体重,并按0 - 4分(0 =最差,4 =最好)对不同身体部位(颈部、胸部、翅膀、通风口和尾部)的羽毛状况进行评分。此外,还对29周龄雏鸡的食羽行为和产蛋性能标准(母鸡日产蛋率%、蛋重、日采食量和FCR)进行了测试。与我们的预期相反,HFP鸟在39周龄时的羽毛状况明显好于LFP鸟。羽翼和尾羽在两个年龄阶段的羽毛得分存在差异。亚组之间在两个年龄段的体重都没有差异。在铅笔测试中也没有差异。但HFP鸟类的羽毛被吃掉的数量明显高于LFP鸟类。这与许多其他研究结果一致。但这种差异并不像之前的研究报道的那么大。与我们的预期相反,亚组之间在生产性能、采食量和饲料转化率方面没有显著差异,尽管在39周龄时,LFP鸟类的羽毛状况明显差于HFP鸟类。但在亚组内,羽毛评分、生产性能、采食量和肉重比均呈负相关。显然,啄羽毛、恐惧、羽毛状况和表现标准之间没有一致的关系。
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引用次数: 2
Einfluss von Biomin®IMBO auf die humorale immunantwort von broilern 影响Biomin®IMBO broilern humorale免疫反应的问题的
Pub Date : 2015-01-01 DOI: 10.1399/eps.2015.111
S. M. Dibaji, Alireza Seidavi, Leila Asadpour, F. S. Chopa, Vito Laudadio, Elisabetta Casalino, V.Dibaji Tufarelli
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引用次数: 1
Laying hen performance in different production systems; why do they differ and how to close the gap? Results of discussions with groups of farmers in The Netherlands, Switzerland and France, benchmarking and model calculations 不同生产体系下蛋鸡生产性能的研究他们为什么会有不同,如何缩小差距?与荷兰、瑞士和法国的农民小组讨论的结果,基准和模型计算
Pub Date : 2014-07-15 DOI: 10.1399/EPS.2014.53
F. Leenstra, V. Maurer, F. Galea, M. Bestman, Z. Amsler, J. Visscher, I. Vermeij, M. V. Krimpen
Free range and organic systems expose the laying hen more to unexpected events and adverse climatic conditions than barn and cage systems. In France, The Netherlands and Switzerland the requirements for a hen suitable to produce in free range and organic systems were discussed with farmers. The farmers preferred for these systems a more ‘robust’ hen, more specifically defined as a heavier hen with good eating capacity. Benchmarking of flocks in a web-based management program in The Netherlands from layer flocks finished in 2008 – 2013 indicated that in earlier years indeed mortality among organic and to a lesser extent free range hens was higher than among barn or cage hens. Feed conversion (kg feed/kg eggs) is higher, but the gap is closing. Improvements in management of the hens during rearing and in the layer phase in free range and organic systems seem to be important. Breeding companies take behaviour and performance in non-cage systems into account in their selection programs. Heavier hens need a diet with a lower protein to energy ratio. From model calculations we concluded that in organic systems a heavier hen might be economically profitable, as total feed costs are lower for the heavier hen then for a hen with a lower body weight requiring a diet with a high protein content. For conventional free range hens this is not the case as then the protein content can be adapted by synthetic amino acids. Field studies and cooperation between farmers and breeding organisations will have to show if a strain of heavier hens will be successful in the rather small organic market.
自由放养和有机系统比谷仓和笼子系统更容易使蛋鸡暴露于意外事件和不利的气候条件下。在法国、荷兰和瑞士,与农民讨论了适合在自由放养和有机系统中生产的母鸡的要求。在这些系统中,农民更喜欢更“健壮”的母鸡,更具体地定义为更重的母鸡,具有良好的进食能力。在荷兰的一个基于网络的管理项目中,对2008年至2013年完成的蛋鸡进行了基准测试,结果表明,在早些年,有机蛋鸡和较小程度上自由放养的蛋鸡的死亡率确实高于笼养蛋鸡。饲料转化率(公斤饲料/公斤蛋)较高,但差距正在缩小。在自由放养和有机系统中,改进母鸡饲养和产蛋期的管理似乎很重要。养殖公司在选择方案中考虑了非笼养系统的行为和性能。较重的母鸡需要较低蛋白质能量比的日粮。从模型计算中,我们得出结论,在有机系统中,体重较重的母鸡可能在经济上有利可图,因为体重较重的母鸡的饲料总成本低于体重较轻的母鸡,后者需要高蛋白质含量的饲料。对于传统的散养母鸡来说,情况并非如此,因为它们的蛋白质含量可以通过合成氨基酸来调节。农民和养殖组织之间的实地研究和合作将表明,一种较重的母鸡是否能在相当小的有机市场上取得成功。
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引用次数: 35
The phenotypic interrelationships between feather pecking, being feather pecked and fear criteria in White Leghorn lines selected for high and low severe feather pecking and their F2-crosses 高、低严重啄羽和被啄羽与恐惧标准之间的表型相互关系及其f2杂交
Pub Date : 2014-06-30 DOI: 10.1399/EPS.2014.50
S. Bögelein, D. M. Hurtado, J. Kjaer, Grashorn, J. Bennewitz, W. Bessei
On the basis of observations that flocks of chickens with high incidence of feather pecking and feather damages show a high fear level, it is generally assumed that feather pecking and fear are positively correlated. This hypothesis was tested in two experiments using adult laying hens of lines selected for high (HFP) and low feather pecking behaviour (LFP) and their reciprocal crosses. A total of 60 adult birds, 30 HFP and 30 LFP, of the selection lines were used in part one of the experiment. The birds were first observed for the number of bouts of severe feather pecks delivered (FPD) and received (FPR) when kept in groups of equal numbers of both lines. Thereafter all birds were subjected to several fear tests: Tonic immobility test (TI), open- field test (OF), emerge box test (ET) and pencil test. In part two of the experiment a total of 967 birds of the F2- crosses of both lines were used. All birds were tested using the same fear tests as above at 7 days and 40 weeks of age. FPD and FPR were observed in adults only. The whole population was split for FPD in HFP and LFP using the threshold of ≥ 2 (HFP) and < 2 (LFP). HFP and LFP of the selection lines and the F2- crosses clearly differed in FPD. LFP of the selection lines received more feather pecks than HFP. There was no significant difference for FPR in HFP and LFP in the F2- crosses. In contrast to our expectation HFP from the selection lines showed a significant shorter duration of TI, shorter latency to move and to vocalize in the OF and a shorter latency to leave the emerge box, indicating lower fear. Similar results were found in the HFP and LFP of the F2- crosses for the duration of TI and latency of head appearance in the ET. Latency of the first step and to vocalize in the OF, however showed the opposite tendency. Line by age interactions appeared for the number of inductions in the TI and the latency of head emerge in the ET. There were no differences between HFP and LFP in the pencil test in both experiments. The phenotypic correlations between FPD and FPR with all fear criteria were low and not significant in both experiments. There is obviously no consistent relationship between feather pecking and fear in this population. Depending on type of fear test and age the HFP may show higher, lower or no difference in fear. Genotypes by age interactions further contribute to the variability of the results. The low phenotypic correlations among the criteria confirm this conclusion.
根据观察,啄毛和羽毛损伤发生率高的鸡群表现出较高的恐惧水平,一般认为啄毛与恐惧呈正相关。这一假设在两个试验中得到了验证,试验对象是选择高啄羽行为(HFP)和低啄羽行为(LFP)品系的成年蛋鸡及其互交。第一部分试验选用60只成鸟,其中高成鸟30只,低成鸟30只。首先观察这些鸟在两种线的数量相等的情况下,严重啄羽毛的次数(FPD)和接受(FPR)。然后,对所有的鸟进行了几项恐惧试验:强直静止试验(TI)、空地试验(OF)、出箱试验(ET)和铅笔试验。第二部分试验选用两系F2杂交组合967只。在7日龄和40周龄时,对所有雏鸟进行相同的恐惧测试。FPD和FPR仅在成人中观察到。采用HFP≥2 (HFP)和LFP < 2 (LFP)的阈值对整个人群进行FPD划分。选择系和F2杂交的HFP和LFP在FPD上存在明显差异。LFP的选育系比HFP的选育系得到更多的羽突。在F2-杂交中,HFP和LFP的FPR无显著差异。与我们的预期相反,来自选择线的HFP显示出明显较短的TI持续时间,较短的在of中移动和发声的延迟时间,较短的离开出现框的延迟时间,表明较低的恐惧。F2-杂交体的HFP和LFP在等待时间和头部出现潜伏期的变化中也有相似的结果,而在等待时间第一步和发声潜伏期则呈现相反的趋势。随着年龄的增长,TI的诱导次数和ET的头出现潜伏期出现了直线相互作用。在两个实验中,HFP和LFP在铅笔测试中没有差异。在两个实验中,FPD和FPR与所有恐惧标准的表型相关性都很低且不显著。在这个种群中,啄羽毛和恐惧之间显然没有一致的关系。根据恐惧测试的类型和年龄,HFP在恐惧方面可能表现出更高、更低或没有差异。年龄相互作用的基因型进一步增加了结果的可变性。这些标准之间的低表型相关性证实了这一结论。
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引用次数: 10
Effect of Fusarium toxin contaminated wheat on health, nutrient digestibility and semen quality of adult cockerels 小麦镰刀菌毒素对成年公鸡健康、营养物质消化率和精液质量的影响
Pub Date : 2014-06-11 DOI: 10.1399/EPS.2014.47
Mohammad Ebrahem, S. Kersten, C. Ehling, H. Valenta, U. Taylor, U. Baulain, G. Breves, A. Beineke, K. Hermeyer, S. Dänicke
A ten-month-feeding trial was conducted with twenty four adult cockerels of a commercial strain “New Hampshire hybrids” to evaluate the effect of feeding of wheat, mainly contaminated with the Fusarium toxin deoxynivalenol, on cockerel’s health, nutrient digestibility, semen quality and DON metabolism. Birds were individually weighed and randomly assigned to one of three treatment groups (control, 4.7 mg DON/kg and 11 mg DON/kg). Feed intake and cockerels’ body weight were not affected by the dietary treatment, nor were the relative organ weights affected. On the other hand, breast muscle relative weight was increased in the cockerels fed DON contaminated diets, while the relative weight of ileum and caecum were significantly decreased at the same time. The content of urea was significantly increased in plasma of cockerels fed the highest DON concentrations, while the triglyceride concentration was significantly lower in plasma of cockerels fed 4.7 mg/kg DON. Haematological and other clinical-chemical parameters remained unaffected. Moreover, crude fat utilization was progressively increased as the concentrations of DON in the diet increased. Utilizations of organic matter, carbohydrates and nitrogen balance were not affected by the dietary treatment. Furthermore, the long term feeding of cockerels with DON contaminated wheat had no negative impact on semen parameters. Taken together, it might be concluded that cockerels are quite resistant to the effects of DON regarding reproductive traits, nutrient digestibility and feed intake. The dose-dependent alterations in the relative weights of breast muscle and digestive organs require further consideration.
采用24只“新汉普郡”商品品系的成年公鸡进行了10个月的饲养试验,以评价饲喂镰刀菌毒素脱氧雪腐镰刀菌醇为主污染的小麦对公鸡健康、营养物质消化率、精液质量和DON代谢的影响。分别称重,随机分为3个处理组(对照组,4.7 mg DON/kg和11 mg DON/kg)。饲粮处理不影响采食量和体重,也不影响相对器官重量。另一方面,DON污染饲粮增加了胸肌的相对重量,同时显著降低了回肠和盲肠的相对重量。饲喂最高DON浓度的公鸡血浆尿素含量显著升高,而饲喂4.7 mg/kg DON的公鸡血浆甘油三酯浓度显著降低。血液学和其他临床化学参数未受影响。此外,随着饲料中DON浓度的增加,粗脂肪利用率逐渐增加。饲料处理对有机质利用、碳水化合物和氮平衡没有影响。此外,长期喂食DON污染的小麦对公鸡的精液参数没有负面影响。综上所述,在生殖性状、营养物质消化率和采食量方面,小公鸡对DON的影响具有较强的抵抗力。乳房肌肉和消化器官相对重量的剂量依赖性改变需要进一步考虑。
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引用次数: 1
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Archiv Fur Geflugelkunde
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