O. Khudyi, R. Kolman, L. Khuda, M. Marchenko, L. Terteryan
Abstract A broodstock was created with individuals from the diminishing natural sterlet, Acipenser ruthenus L., population in the upper area of the Dniester basin. Selects and spawners were reared in flow-through tanks with natural thermal regimes. This is the first time artificial reproduction has been performed with this species, followed by incubation and larval rearing. The experimental rearing was conducted in tanks in a recirculating aquaculture system, in which the basic environmental quality parameters were controlled, including water temperature, oxygen saturation, and levels of ammonia and nitrite. During rearing, growth rates and proximate composition of larvae and then juvenile were examined. It was confirmed that during the period of endogenous feeding there was a two-fold decrease in the contents of protein and lipids in the bodies of the larvae. The effects of feeding the sterlet juvenile two different types of commercial feed were compared, and it was confirmed that the feed had a significant impact on sterlet growth rates. The mean daily increase in the body weight of sterlet juvenile fed Skretting feed was 1.91%, while that in the group fed Aller feed was 0.97%. Additionally, the juvenile from the former group was characterized by higher contents of protein and lipids in both the liver and muscle and the fatty acid profile was more varied.
{"title":"Characterization of growth and biochemical composition of sterlet, Acipenser ruthenus L., juveniles from the Dniester population reared in RAS","authors":"O. Khudyi, R. Kolman, L. Khuda, M. Marchenko, L. Terteryan","doi":"10.2478/aopf-2014-0026","DOIUrl":"https://doi.org/10.2478/aopf-2014-0026","url":null,"abstract":"Abstract A broodstock was created with individuals from the diminishing natural sterlet, Acipenser ruthenus L., population in the upper area of the Dniester basin. Selects and spawners were reared in flow-through tanks with natural thermal regimes. This is the first time artificial reproduction has been performed with this species, followed by incubation and larval rearing. The experimental rearing was conducted in tanks in a recirculating aquaculture system, in which the basic environmental quality parameters were controlled, including water temperature, oxygen saturation, and levels of ammonia and nitrite. During rearing, growth rates and proximate composition of larvae and then juvenile were examined. It was confirmed that during the period of endogenous feeding there was a two-fold decrease in the contents of protein and lipids in the bodies of the larvae. The effects of feeding the sterlet juvenile two different types of commercial feed were compared, and it was confirmed that the feed had a significant impact on sterlet growth rates. The mean daily increase in the body weight of sterlet juvenile fed Skretting feed was 1.91%, while that in the group fed Aller feed was 0.97%. Additionally, the juvenile from the former group was characterized by higher contents of protein and lipids in both the liver and muscle and the fatty acid profile was more varied.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"19 1","pages":"249 - 256"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84795692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kozłowski, M. Szczepkowski, K. Wunderlich, B. Szczepkowska, I. Piotrowska
Abstract The experiment examined the possibility of rearing juvenile pikeperch, Sander lucioperca (L.) in polyculture with sterlet Acipenser ruthenus L. in a recirculating system. Three variants of pikeperch rearing were tested: monoculture (group S), with the addition of sterlet at 10% (group S10) and 20% (group S20) of the initial pikeperch biomass. After 56 days of rearing, no differences in the growth rates or survival of the pikeperch were noted. The value of the feed conversion ratio in the monoculture group was 1.19 and was significantly statistically higher than in the polyculture groups, the values of which were 0.84 (S10) and 0.74 (S20). The mean oxygen consumption and ammonia excretion values did not differ significantly statistically among the studied groups. Including the sterlet stock with the pikeperch permitted obtaining the additional value of the sterlet biomass using the same quantity of feed. Additionally, the inclusion of sterlet decreased the labor intensity of pikeperch rearing since the tanks did not need cleaning as frequently.
{"title":"Polyculture of juvenile pikeperch (Sander lucioperca (L.)) and sterlet (Acipenser ruthenus L.) in a recirculating system","authors":"M. Kozłowski, M. Szczepkowski, K. Wunderlich, B. Szczepkowska, I. Piotrowska","doi":"10.2478/aopf-2014-0024","DOIUrl":"https://doi.org/10.2478/aopf-2014-0024","url":null,"abstract":"Abstract The experiment examined the possibility of rearing juvenile pikeperch, Sander lucioperca (L.) in polyculture with sterlet Acipenser ruthenus L. in a recirculating system. Three variants of pikeperch rearing were tested: monoculture (group S), with the addition of sterlet at 10% (group S10) and 20% (group S20) of the initial pikeperch biomass. After 56 days of rearing, no differences in the growth rates or survival of the pikeperch were noted. The value of the feed conversion ratio in the monoculture group was 1.19 and was significantly statistically higher than in the polyculture groups, the values of which were 0.84 (S10) and 0.74 (S20). The mean oxygen consumption and ammonia excretion values did not differ significantly statistically among the studied groups. Including the sterlet stock with the pikeperch permitted obtaining the additional value of the sterlet biomass using the same quantity of feed. Additionally, the inclusion of sterlet decreased the labor intensity of pikeperch rearing since the tanks did not need cleaning as frequently.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"48 1","pages":"237 - 242"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79752097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The aim of the present study was to perform quantitative and qualitative evaluations of developing blood cells in the head kidney of European chub, Squalius cephalus (L.). Twenty-one types of hematopoietic precursors and mature cells were identified in head kidneys isolated from ten juvenile fish. The lymphoid lineage was the most abundant, followed by granuloid, erythroid, thrombocytoid, blast, and monocytoid cells. The lymphocyte was the most frequently occurring cell in the kidney and the most numerous of the lymphoid cells. The neutrophilic lineage was the most numerous among the granuloid cells. The erythropoietic series comprised five stages (erythroblasts: basophilic, polychromatic, orthochromatic; young and mature erythrocytes), more than the numbers of developmental stages of any other lineages. Juvenile cells were more abundant than mature stages in the head kidney. Only mature eosinophils and thrombocytes were found in chub hematopoietic tissue. The cellular composition in chub hematopoietic tissue was similar to that in other fish species.
{"title":"Cell composition of the head kidney of European chub (Squalius cephalus L. )","authors":"E. Kondera","doi":"10.2478/aopf-2014-0029","DOIUrl":"https://doi.org/10.2478/aopf-2014-0029","url":null,"abstract":"Abstract The aim of the present study was to perform quantitative and qualitative evaluations of developing blood cells in the head kidney of European chub, Squalius cephalus (L.). Twenty-one types of hematopoietic precursors and mature cells were identified in head kidneys isolated from ten juvenile fish. The lymphoid lineage was the most abundant, followed by granuloid, erythroid, thrombocytoid, blast, and monocytoid cells. The lymphocyte was the most frequently occurring cell in the kidney and the most numerous of the lymphoid cells. The neutrophilic lineage was the most numerous among the granuloid cells. The erythropoietic series comprised five stages (erythroblasts: basophilic, polychromatic, orthochromatic; young and mature erythrocytes), more than the numbers of developmental stages of any other lineages. Juvenile cells were more abundant than mature stages in the head kidney. Only mature eosinophils and thrombocytes were found in chub hematopoietic tissue. The cellular composition in chub hematopoietic tissue was similar to that in other fish species.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"145 1","pages":"271 - 280"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79935475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Light and color have effect physiological aspects of fish such as growth, the neuro-hormonal system, and reproduction. In the present study, the effects of light color and photoperiod on the levels of selected parameters of coelomic fluid (glucose, total protein, cholesterol, calcium, magnesium) of female sterlet (Acipenser ruthenus) were investigated. Thirty-six broodstock were kept in 12 experimental tanks (500 l) for six months. The fish were reared under four different light regimes (two photoperiods of 18L:6D and 6L:18D and two colors - red and blue) at an intensity of 150 Lx (three replicates). Fish reared under the red-long photoperiod had the highest concentration of the selected parameters of the coelomic fluid. Moreover, significant differences were noted in the all the parameters in fish reared under the red-long photoperiod in comparison with fish from the other treatments (P < 0.05). The results indicate that light (color and photoperiod) influences egg quality during the final stage of A. ruthenus reproduction.
{"title":"Selected coelomic fluid parameters of sterlet, Acipenser ruthenus L.: effects of light color and photoperiod","authors":"Hajar Azarin, M. Aramli, F. Naderi","doi":"10.2478/aopf-2014-0025","DOIUrl":"https://doi.org/10.2478/aopf-2014-0025","url":null,"abstract":"Abstract Light and color have effect physiological aspects of fish such as growth, the neuro-hormonal system, and reproduction. In the present study, the effects of light color and photoperiod on the levels of selected parameters of coelomic fluid (glucose, total protein, cholesterol, calcium, magnesium) of female sterlet (Acipenser ruthenus) were investigated. Thirty-six broodstock were kept in 12 experimental tanks (500 l) for six months. The fish were reared under four different light regimes (two photoperiods of 18L:6D and 6L:18D and two colors - red and blue) at an intensity of 150 Lx (three replicates). Fish reared under the red-long photoperiod had the highest concentration of the selected parameters of the coelomic fluid. Moreover, significant differences were noted in the all the parameters in fish reared under the red-long photoperiod in comparison with fish from the other treatments (P < 0.05). The results indicate that light (color and photoperiod) influences egg quality during the final stage of A. ruthenus reproduction.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"25 1","pages":"243 - 247"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87252930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Ulikowski, I. Piotrowska, Łucjan Chybowski, T. Krzywosz, P. Traczuk
Abstract Interactions were studied among juvenile narrow-claw crayfish, Astacus leptodactylus (Eschscholtz), and common water frog, Rana esculenta (L.), tadpoles and common blue damselfly, Enallagma cyathigerum (Charpentier), larvae during rearing under controlled conditions. Interactions among the species studied had a positive impact on the survival of the crayfish, but the differences were not statistically significant (P ≥ 0.5). The juvenile crayfish attacked and consumed the frog tadpoles and damselflies, but the juvenile crayfish very rarely fell prey to them. Only in the initial stage of life and during molting did larval damselflies prey upon juvenile crayfish. After 30 days of the experiment the interaction between crayfish-tadpoles and crayfish-larval damselflies was not noted to have had a statistically significant (P ≥ 0.05) impact on crayfish growth. Juvenile crayfish aggression toward tadpoles and larval damselflies was often offset by the loss of even both chelipeds. In the crayfish-larval damselfly interaction the loss of both chelipeds was three-fold more common than it was in the crayfish-tadpole interaction; however, these differences were not statistically significant (P ≥ 0.5). The effect of intraspecific interaction (crayfish-crayfish) was more a threat in terms of mortality from cannibalism than were interspecific interactions (crayfish-tadpole and crayfish-larval damselfly).
{"title":"Interaction between juvenile narrow-claw crayfish, Astacus leptodactylus (Eschscholtz), and common water frog, Rana esculenta (L.), tadpoles or common blue damselfly, Enallagma cyathigerum (Charpentier), larvae during rearing under controlled conditions","authors":"D. Ulikowski, I. Piotrowska, Łucjan Chybowski, T. Krzywosz, P. Traczuk","doi":"10.2478/aopf-2014-0027","DOIUrl":"https://doi.org/10.2478/aopf-2014-0027","url":null,"abstract":"Abstract Interactions were studied among juvenile narrow-claw crayfish, Astacus leptodactylus (Eschscholtz), and common water frog, Rana esculenta (L.), tadpoles and common blue damselfly, Enallagma cyathigerum (Charpentier), larvae during rearing under controlled conditions. Interactions among the species studied had a positive impact on the survival of the crayfish, but the differences were not statistically significant (P ≥ 0.5). The juvenile crayfish attacked and consumed the frog tadpoles and damselflies, but the juvenile crayfish very rarely fell prey to them. Only in the initial stage of life and during molting did larval damselflies prey upon juvenile crayfish. After 30 days of the experiment the interaction between crayfish-tadpoles and crayfish-larval damselflies was not noted to have had a statistically significant (P ≥ 0.05) impact on crayfish growth. Juvenile crayfish aggression toward tadpoles and larval damselflies was often offset by the loss of even both chelipeds. In the crayfish-larval damselfly interaction the loss of both chelipeds was three-fold more common than it was in the crayfish-tadpole interaction; however, these differences were not statistically significant (P ≥ 0.5). The effect of intraspecific interaction (crayfish-crayfish) was more a threat in terms of mortality from cannibalism than were interspecific interactions (crayfish-tadpole and crayfish-larval damselfly).","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"12 1","pages":"257 - 264"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81977931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Most research to date on the status of the river lamprey, Lampetra fluviatilis L. in Latvia has been done based on catch statistics data. The aim of this study was to explore the present status of the river lamprey fishery in Latvia to improve the understanding of catch statistics data. Currently, river lamprey fishing in Latvia is carried out at 24 fishing grounds located on 17 rivers. The most popular fishing gear is the lamprey fyke net, but lamprey weirs and lamprey trammel nets are also used. The type and design of the fishing gear depends on the parameters of the fishing ground. The catch size is highly variable and is greatly affected by the number of non-resources related circumstances, such as fishing regulations and meteorological factors determining the intensity of lamprey migration during the fishing season and opportunities for fishing during periods of the most intense migration. The fishing effort and the type of fishing gear have also largely changed since the 1960s and 1970s. Therefore, the fluctuation in both the long- and short-term catch data may not correspond to changes in the actual status of the lamprey population.
{"title":"River lamprey, Lampetra fluviatilis L., fishery in Latvia – insight into the origin of catch statistics data","authors":"Kaspar Abersons, J. Birzaks","doi":"10.2478/aopf-2014-0017","DOIUrl":"https://doi.org/10.2478/aopf-2014-0017","url":null,"abstract":"Abstract Most research to date on the status of the river lamprey, Lampetra fluviatilis L. in Latvia has been done based on catch statistics data. The aim of this study was to explore the present status of the river lamprey fishery in Latvia to improve the understanding of catch statistics data. Currently, river lamprey fishing in Latvia is carried out at 24 fishing grounds located on 17 rivers. The most popular fishing gear is the lamprey fyke net, but lamprey weirs and lamprey trammel nets are also used. The type and design of the fishing gear depends on the parameters of the fishing ground. The catch size is highly variable and is greatly affected by the number of non-resources related circumstances, such as fishing regulations and meteorological factors determining the intensity of lamprey migration during the fishing season and opportunities for fishing during periods of the most intense migration. The fishing effort and the type of fishing gear have also largely changed since the 1960s and 1970s. Therefore, the fluctuation in both the long- and short-term catch data may not correspond to changes in the actual status of the lamprey population.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"8 1","pages":"169 - 179"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87870836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The aim of the study was to determine the degree of overlap in the feeding niches of early developmental stages of roach, Rutilus rutilus (L.), perch, Perca fluviatilis L., bleak, Alburnus alburnus (L.) and sunbleak, Leucaspius delineatus (Heckel). The hypotheses were that (i) larvae of different fish species of similar body sizes feed on similar food, and that (ii) smaller fish compete with larger ones for similar prey. The results of the analysis was to answer the question of whether the diet overlap of juvenile fish stages decreases with ontogenetic development. The study was conducted for two years (2001-2002) in two shallow, polymictic, eutrophic lakes. The diets of early developmental stages of fish species of similar body sizes differed. The few instances of diet overlap resulted from the abundance of food resources in the environment. However, fish of smaller body sizes exploited the same food resources as did individuals of the same species that belonged to different cohorts.
{"title":"Does diet overlap among larval and 0+ fish species decrease with ontogenetic development?","authors":"E. Bogacka-Kapusta, A. Kapusta","doi":"10.2478/aopf-2014-0022","DOIUrl":"https://doi.org/10.2478/aopf-2014-0022","url":null,"abstract":"Abstract The aim of the study was to determine the degree of overlap in the feeding niches of early developmental stages of roach, Rutilus rutilus (L.), perch, Perca fluviatilis L., bleak, Alburnus alburnus (L.) and sunbleak, Leucaspius delineatus (Heckel). The hypotheses were that (i) larvae of different fish species of similar body sizes feed on similar food, and that (ii) smaller fish compete with larger ones for similar prey. The results of the analysis was to answer the question of whether the diet overlap of juvenile fish stages decreases with ontogenetic development. The study was conducted for two years (2001-2002) in two shallow, polymictic, eutrophic lakes. The diets of early developmental stages of fish species of similar body sizes differed. The few instances of diet overlap resulted from the abundance of food resources in the environment. However, fish of smaller body sizes exploited the same food resources as did individuals of the same species that belonged to different cohorts.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"96 1","pages":"221 - 228"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76471410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The aim of study was to determine the effects of exposure to the product DEZYNFEKTANT-CIP (Eng. - Disinfectant-CIP), which is formulated with peracetic acid and hydrogen peroxide, on oxidative stress biomarkers (lipid peroxidation (LPO) levels and the carbonyl content of oxidatively modified proteins) and antioxidant defenses (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), total antioxidant capacity) in muscle, gill, hepatic, and cardiac tissues of rainbow trout, Oncorhynchus mykiss (Walbaum). LPO and carbonyl contents changed with tissue type. Exposure to Disinfectant-CIP led to a significant decrease in LPO in muscle tissues and carbonyl content in muscle and gill tissues. The inhibition of SOD and CAT activity in muscle, hepatic, and cardiac tissues was observed probably because of increased oxidative stress during disinfection; however, hepatic and cardiac GPx activity increased in an attempt to counteract oxidative stress. We suggest that oxidative stress during the oxidation of peracetic acid and hydrogen peroxide could be counteracted by the antioxidant system in trout tissues. Correlative analysis between oxidative stress biomarkers and antioxidant defense confirms the pivotal role of SOD and CAT against CIP-induced oxidative stress
{"title":"Oxidative stress biomarkers in different tissues of rainbow trout (Oncorhynchus mykiss) exposed to Disinfectant-CIP formulated with peracetic acid and hydrogen peroxide","authors":"H. Tkachenko, N. Kurhaluk, J. Grudniewska","doi":"10.2478/aopf-2014-0021","DOIUrl":"https://doi.org/10.2478/aopf-2014-0021","url":null,"abstract":"Abstract The aim of study was to determine the effects of exposure to the product DEZYNFEKTANT-CIP (Eng. - Disinfectant-CIP), which is formulated with peracetic acid and hydrogen peroxide, on oxidative stress biomarkers (lipid peroxidation (LPO) levels and the carbonyl content of oxidatively modified proteins) and antioxidant defenses (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), total antioxidant capacity) in muscle, gill, hepatic, and cardiac tissues of rainbow trout, Oncorhynchus mykiss (Walbaum). LPO and carbonyl contents changed with tissue type. Exposure to Disinfectant-CIP led to a significant decrease in LPO in muscle tissues and carbonyl content in muscle and gill tissues. The inhibition of SOD and CAT activity in muscle, hepatic, and cardiac tissues was observed probably because of increased oxidative stress during disinfection; however, hepatic and cardiac GPx activity increased in an attempt to counteract oxidative stress. We suggest that oxidative stress during the oxidation of peracetic acid and hydrogen peroxide could be counteracted by the antioxidant system in trout tissues. Correlative analysis between oxidative stress biomarkers and antioxidant defense confirms the pivotal role of SOD and CAT against CIP-induced oxidative stress","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"14 1","pages":"207 - 219"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85938161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Georgieva, S. Stoyanova, I. Velcheva, T. Vasileva, V. Bivolarski, I. Iliev, V. Yancheva
Abstract The present study aimed to evaluate the current contamination status of Topolnitsa Reservoir, which is located in a region with intensive copper mining. The reservoir has been continually contaminated with metals over the last few decades. As, Cd, Cu, Ni, Pb, and Zn concentrations were measured in surface water samples and in the liver of common rudd, Scardinius erythrophthalmus (L.), in three different seasons: spring, summer, and autumn. The morphological structure of the fish liver was examined and the hepatic LDH, ALAT, and ASAT activities were measured. In general, metal concentrations in the water varied, but As and Cu were present in all three seasons at levels higher than the maximum permissible levels set by law. The metal concentrations in the fish liver were significantly higher than in the water. Histological alterations were classified as degenerative changes (granular, vacuolar, hydropic, and fatty degeneration), necrotic changes (necrobiosis) - karyopyknosis, karryorehsis, and karyolyzis, and necrosis)); and changes in blood vessels (hyperemia in sinusoids and major blood vessels). Higher LDH, ALAT, and ASAT activities in fish livers were measured in comparison to reference fish. In addition, the ALAT activity in the livers of S. erythrophthalmus from Topolnitsa Reservoir was significantly higher in the summer. Overall, it can be concluded that the metal-contaminated waters of Topolnitsa Reservoir lead to negative changes in the common rudd tissues at the cellular level including impaired structure and enhanced enzymatic activity in the fish liver.
摘要本研究旨在评价Topolnitsa水库的污染现状，该水库位于铜矿密集开采地区。在过去的几十年里，这个水库一直受到金属污染。测定了春、夏、秋3个季节地表水样品和红眼红鲌肝脏中As、Cd、Cu、Ni、Pb、Zn的浓度。观察了鱼肝脏的形态结构，测定了肝脏LDH、ALAT和ASAT活性。一般来说，水中的金属浓度各不相同，但砷和铜在所有三个季节的含量都高于法律规定的最高允许水平。鱼肝中的金属含量明显高于水中。组织学改变分为退行性改变(颗粒性、空泡性、积水性和脂肪性变性)、坏死性改变(坏死性)——核固缩、核疏松、核溶解和坏死);血管的变化(窦状动脉和大血管充血)。与参考鱼相比，鱼肝脏中LDH、ALAT和ASAT活性更高。此外，在夏季，来自Topolnitsa水库的红眼S. S. erythrophthalmus肝脏中的ALAT活性显著升高。综上所述，Topolnitsa水库的金属污染水在细胞水平上导致了鱼常见rudd组织的负面变化，包括结构受损和酶活性增强。
{"title":"Metal effects on histological and biochemical parameters of common rudd (Scardinius erythrophthalmus L.)","authors":"E. Georgieva, S. Stoyanova, I. Velcheva, T. Vasileva, V. Bivolarski, I. Iliev, V. Yancheva","doi":"10.2478/aopf-2014-0020","DOIUrl":"https://doi.org/10.2478/aopf-2014-0020","url":null,"abstract":"Abstract The present study aimed to evaluate the current contamination status of Topolnitsa Reservoir, which is located in a region with intensive copper mining. The reservoir has been continually contaminated with metals over the last few decades. As, Cd, Cu, Ni, Pb, and Zn concentrations were measured in surface water samples and in the liver of common rudd, Scardinius erythrophthalmus (L.), in three different seasons: spring, summer, and autumn. The morphological structure of the fish liver was examined and the hepatic LDH, ALAT, and ASAT activities were measured. In general, metal concentrations in the water varied, but As and Cu were present in all three seasons at levels higher than the maximum permissible levels set by law. The metal concentrations in the fish liver were significantly higher than in the water. Histological alterations were classified as degenerative changes (granular, vacuolar, hydropic, and fatty degeneration), necrotic changes (necrobiosis) - karyopyknosis, karryorehsis, and karyolyzis, and necrosis)); and changes in blood vessels (hyperemia in sinusoids and major blood vessels). Higher LDH, ALAT, and ASAT activities in fish livers were measured in comparison to reference fish. In addition, the ALAT activity in the livers of S. erythrophthalmus from Topolnitsa Reservoir was significantly higher in the summer. Overall, it can be concluded that the metal-contaminated waters of Topolnitsa Reservoir lead to negative changes in the common rudd tissues at the cellular level including impaired structure and enhanced enzymatic activity in the fish liver.","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"68 1","pages":"197 - 206"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89247784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Ghahremanzadeh, J. I. Namin, A. Bani, A. Hallajian
Abstract The size and number of chloride cells and serum ion concentrations in kutum, Rutillus frisii kutum Nordman, from brackish (Caspian Sea) and fresh water (Khoshkrood River) environments were studied to gain a better understanding of osmoregulation in this species. Twenty mature kutum specimens were collected from the Caspian Sea (Anzali coasts, 8.49 ppt salinity and 12.4°C temperature) and 20 specimens from Khoshkrood River (0.18 ppt salinity and 18°C temperature). Gill samples were analyzed histologically and concentrations of Na+, Cl- , K+, and Mg2+ ions were determined in the blood serum. Concentrations of Na+, Cl- , K+, and Mg2+ ions and osmotic pressure in mature kutum from brackish water were significantly higher than in specimens from fresh water. The average size and number of chloride cells in the fish from seawater were considerably larger than those from fresh water. The mean size of chloride cells was 6.89 ± 1.16 μm in brackish water samples and 5.1 ± 0.81 μm in river samples. The average number of chloride cells in brackish and river water samples were 16.92 and 6.57, respectively. The density and size of chloride cells increased with increases in salinity
{"title":"Cytological comparison of gill chloride cells and blood serum ion concentrations in kutum (Rutilus frisii kutum) spawners from brackish (Caspian Sea) and fresh water (Khoshkrood River) environments","authors":"Z. Ghahremanzadeh, J. I. Namin, A. Bani, A. Hallajian","doi":"10.2478/aopf-2014-0019","DOIUrl":"https://doi.org/10.2478/aopf-2014-0019","url":null,"abstract":"Abstract The size and number of chloride cells and serum ion concentrations in kutum, Rutillus frisii kutum Nordman, from brackish (Caspian Sea) and fresh water (Khoshkrood River) environments were studied to gain a better understanding of osmoregulation in this species. Twenty mature kutum specimens were collected from the Caspian Sea (Anzali coasts, 8.49 ppt salinity and 12.4°C temperature) and 20 specimens from Khoshkrood River (0.18 ppt salinity and 18°C temperature). Gill samples were analyzed histologically and concentrations of Na+, Cl- , K+, and Mg2+ ions were determined in the blood serum. Concentrations of Na+, Cl- , K+, and Mg2+ ions and osmotic pressure in mature kutum from brackish water were significantly higher than in specimens from fresh water. The average size and number of chloride cells in the fish from seawater were considerably larger than those from fresh water. The mean size of chloride cells was 6.89 ± 1.16 μm in brackish water samples and 5.1 ± 0.81 μm in river samples. The average number of chloride cells in brackish and river water samples were 16.92 and 6.57, respectively. The density and size of chloride cells increased with increases in salinity","PeriodicalId":8293,"journal":{"name":"Archives of Polish Fisheries","volume":"87 1","pages":"189 - 196"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86814387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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