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Example of the application the microsatellite DNA fragments in the study of farmed European catfish (Silurus glanis, L.) broodstock 微卫星DNA片段在养殖欧洲鲶鱼(Silurus glanis, L.)亲鱼研究中的应用实例
Q3 Agricultural and Biological Sciences Pub Date : 2015-06-01 DOI: 10.1515/aopf-2015-0010
M. Kuciński, T. Liszewski, Anna Krzyoeków, D. Fopp‐Bayat, B. Łączyńska
Abstract European catfish, Silurus glanis L., is the second largest freshwater fish in Europe. The species is very popular among the farmers, as it is one of the most promising European aquaculture species. Despite the growing importance of European catfish in freshwater aquaculture, the genetic data available on this species are still limited. The main purpose of the present study was to develop a reliable, feasible genetic protocol for future studies on European catfish populations and broodstocks in Poland. The genetic characteristics of the tested fish group were based on genetic parameters such as the polymorphism information content (PIC), the effective population size (Ne), the inbreeding coefficient (Fis), and the Garza-Williamson index (M), among others. Additionally, the potential effects of a genetic bottleneck on the genetic variation of the broodstock were examined. The genetic analysis protocol described in this study can be used to establish genetic-based records for European catfish broodstocks, including for sperm cryobanking. This approach will be useful for elaborating the selection procedures that allow for optimal assemblages of spawning pairs in artificial reproduction. The application of the genetic analysis protocol in practice will permit maintaining high quality in European catfish broodstocks.
欧洲鲶鱼(Silurus glanis L.)是欧洲第二大淡水鱼。该品种在农民中很受欢迎,因为它是最有前途的欧洲水产养殖品种之一。尽管欧洲鲶鱼在淡水水产养殖中的重要性日益增加,但有关该物种的遗传数据仍然有限。本研究的主要目的是为今后对波兰的欧洲鲶鱼种群和亲鱼的研究制定一个可靠、可行的遗传方案。通过多态性信息含量(PIC)、有效种群大小(Ne)、近交系数(Fis)和Garza-Williamson指数(M)等遗传参数对受试鱼群进行遗传特征分析。此外,还研究了遗传瓶颈对亲鱼遗传变异的潜在影响。本研究中描述的遗传分析方案可用于建立欧洲鲶鱼亲鱼的遗传记录,包括精子冷冻库。这种方法将有助于制定选择程序,使人工繁殖中产卵对的最佳组合成为可能。遗传分析方案在实践中的应用将使欧洲鲶鱼种源保持高质量。
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引用次数: 0
Cultivating Moina macrocopa Straus in different media using carotenogenic yeast Rhodotorula 利用胡萝卜素酵母红酵母在不同培养基中培养大红豆菌
Q3 Agricultural and Biological Sciences Pub Date : 2015-03-01 DOI: 10.1515/aopf-2015-0004
Olga Kushniryk, O. Khudyi, L. Khuda, R. Kolman, M. Marchenko
Abstract The possibility of using carotenogenic yeast from the genus Rhodotorula as food substrate was studied during the cultivation of Moina macrocopa. The results showed that replacing traditional food substrates during the cultivation of the live fish food M. macrocopa resulted in improved characteristics that were superior to the alternative. Using carotenogenic yeast Rhodotorula glutinis (Fres.) Harrison to stimulate carotenoid accumulation in live feed is better than R. rubra (Demme) Lodder. The optimal duration of enriching M. macrocopa with carotenoids was four days when using various types of yeast capable of carotenogenesis. Replacing the standard medium for culturing M. macrocopa with the alternative medium was less expensive. Breeding live feed on recirculating aquaculture system (RAS) post-production water was proven to be effective.
摘要:本文研究了在培养大红藓菌(Moina macrocopa)过程中,利用红藓属(Rhodotorula)产胡萝卜素酵母作为食物基质的可能性。结果表明,在养殖活鱼饲料中,替代传统的食物基质可获得优于替代的性状改善。利用产胡萝卜素酵母粘胶红酵母(Fres.)哈里森对刺激活饲料中类胡萝卜素积累的作用优于红唇红唇(Demme) loder。当使用不同类型的能够生成胡萝卜素的酵母时,用类胡萝卜素富集巨巨假单胞菌的最佳时间为4天。用替代培养基代替标准培养基培养大孔菌的成本较低。在循环水养殖系统(RAS)生产后水体中养殖活饲料是有效的。
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引用次数: 9
Historical occurrence and extinction of Atlantic salmon in the River Elbe from the fourteenth to the twentieth centuries 14至20世纪易北河大西洋鲑鱼的历史发生和灭绝
Q3 Agricultural and Biological Sciences Pub Date : 2015-03-01 DOI: 10.1515/aopf-2015-0001
Jan Andreska, L. Hanel
Abstract Data on the occurrence, biology, and historical background of the Atlantic salmon, Salmo salar L., (Pisces, Salmoniformes) in the Elbe river basin (Europe, North Sea drainage area) with a focus on Bohemian territory (Central Europe) from the fourteenth to twentieth centuries are summarized in this paper. Historical methods of salmon fishing in Central Europe and historical legal protection of salmon in Bohemia are presented. The salmon is a model example of species which was extirpated as a result of anthropogenic changes in the landscape and rivers in some water systems. The human activities, such as stream bed regulation, dam system construction, other migration barriers, water pollution, fisheries exploitation, that led to the extirpation of Atlantic salmon in the Elbe river basin (are discussed. The last sporadic migrating native salmon were registered in the Bohemian section of the Elbe river basin in the mid twentieth century.
摘要本文综述了14 - 20世纪易北河流域(欧洲,北海流域)以波西米亚地区(中欧)为重点的大西洋鲑鱼Salmo salar L.(双鱼座,鲑形目)的发生、生物学和历史背景。介绍了中欧鲑鱼捕捞的历史方法和波希米亚鲑鱼的历史法律保护。鲑鱼是由于某些水系的景观和河流的人为变化而灭绝的物种的典型例子。讨论了易北河流域大西洋鲑鱼灭绝的原因,包括河床整治、大坝建设、其他迁徙障碍、水污染、渔业开发等。最后一次零星迁徙的本地鲑鱼是在20世纪中期在易北河流域的波西米亚地区登记的。
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引用次数: 1
Histomorphological study of the gut developmental pattern in early life history stages of featherback, Chitala chitala (Hamilton) Chitala Chitala早期生活史阶段肠道发育模式的组织形态学研究
Q3 Agricultural and Biological Sciences Pub Date : 2015-03-01 DOI: 10.1515/aopf-2015-0003
A. Mitra, P. K. Mukhopadhyay, S. Homechaudhuri
Abstract The present study was conducted to observe morphological and histological changes in the developing digestive system during the ontogeny of featherback, Chitala chitala (Hamilton), from hatching to 30 days post hatch (dph). Three significant stages were identified during digestive tract development in featherback: (1) endotrophic (0-8 dph), (2) endoexotrophic (8-12 dph), and (3) exotrophic (12-30 dph). At hatching, the mouth was closed and the digestive tract was a straight tube. At 8 dph, the mouth was opened and the appearance of the esophagus was observed. Between 6-8 dph, the primordial liver and exocrine pancreas were observed. Intestinal enterocyte activity was observed before stomach development. The esophageal goblet cells, teeth, and taste buds developed 2 days after the opening of the mouth, at the time of exogenous feeding. The development of the stomach, gastric glands, and pyloric caeca took place between the 7 and 12 dph. Except the increase in size and complexity of the structures, no noticeable changes were observed after 12 dphin C. chitala during the experiment. Our findings in the current work provide valuable information which might be useful for improving current larval rearing techniques of this promising new candidate species for freshwater aquaculture diversification.
摘要本研究对羽背鸟Chitala Chitala (Hamilton)从孵化到孵化后30 d消化系统发育过程中的形态学和组织学变化进行了观察。在羽背动物的消化道发育过程中,确定了三个重要阶段:(1)内源性(0-8 dph),(2)内源性(8-12 dph)和(3)外源性(12-30 dph)。孵化时,嘴巴紧闭,消化道呈直管状。8 dph时,张开口腔,观察食管外观。在6-8 dph之间,观察原始肝脏和外分泌胰腺。胃发育前观察肠上皮细胞活性。食道杯状细胞、牙齿和味蕾在开口后2天外源性喂养时发育。胃、胃腺和幽门盲肠的发育发生在每小时7 - 12岁之间。实验过程中,除了结构的大小和复杂性增加外,没有明显的变化。我们的研究结果为改善目前淡水养殖多样化的新候选物种的幼虫饲养技术提供了有价值的信息。
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引用次数: 13
Information on huchen, Hucho hucho (L.), from historical Polish sources 关于huchen的信息,Hucho Hucho (L.),来自波兰历史资料
Q3 Agricultural and Biological Sciences Pub Date : 2015-03-01 DOI: 10.1515/aopf-2015-0002
S. Cios
Abstract This paper presents historical information on the Danube huchen, Hucho hucho (L.), in Polish sources from the sixteenth to early twentieth centuries in the Danube, Prut, Dniester, and Vistula river basins. These accounts concern the historical distribution of the species and its economic importance, culinary value, fishing methods, and artificial reproduction and propagation. The occurrence of huchen in the Dniester River basin until the beginning of the eighteenth century merits special interest, because this river is not mentioned by any modern source on the natural distribution of this species. The extinction of the huchen in this river can likely be attributed to anthropogenic changes in the environment that were associated mainly with agricultural development.
摘要本文介绍了多瑙河huchen, Hucho Hucho (L.)的历史信息,在波兰来源从16世纪到20世纪初在多瑙河,普鲁特,德涅斯特和维斯瓦河流域。这些描述涉及该物种的历史分布及其经济重要性、烹饪价值、捕捞方法以及人工繁殖和繁殖。胡晨在德涅斯特河流域的出现直到18世纪初才值得特别关注,因为这条河没有任何现代资料提到该物种的自然分布。这条河中胡臣的灭绝可能是由于与农业发展有关的人为环境变化。
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引用次数: 1
Changes in macrophyte communities in Lake Swarzędzkie after the first year of restoration 恢复一年后Swarzędzkie湖大型植物群落的变化
Q3 Agricultural and Biological Sciences Pub Date : 2015-03-01 DOI: 10.1515/aopf-2015-0005
J. Rosińska, R. Gołdyn
Abstract Lake Swarzędzkie, near Poznań, was a hypertrophic lake because of its high nutrient content, cyanobacteria blooms, and disruptive recreational use, especially swimming, which was popular there. This is why protection measures have been in place since 1991, and a restoration program has been ongoing since fall 2011. The evaluation of the presence and distribution of macrophytes as an important element of lake ecosystem was conducted in August 2012. Nine plant communities were observed: Phragmitetum communis; Typhetum angustifoliae; Nupharo-Nymphaeetum albae; Hydrocharitetum morsus-ranae; Thelypteridi-Phragmitetum; Cicuto-Caricetum pseudocyperi; Acoretum calami; Ceratophylletum demersi; Potametum lucentis. The first three were dominating associations. The presence of submerged vegetation appears to verify the positive impact of the applied conservation and restoration measures. Improvement is confirmed by the significant decrease in concentrations of chlorophyll-a and total nitrogen, as well as the gradual decrease in total suspended solids and increased transparency.
波兹纳斯附近的Swarzędzkie湖是一个肥厚湖泊,因为它的营养含量高,蓝藻大量繁殖,破坏了娱乐用途,特别是游泳,在那里很受欢迎。这就是为什么自1991年以来一直采取保护措施,并自2011年秋季以来一直在进行恢复计划。2012年8月对湖泊生态系统的重要组成部分——大型植物的存在和分布进行了评价。共观察到9个植物群落:芦苇群落(Phragmitetum communis);Typhetum angustifoliae;Nupharo-Nymphaeetum albae;Hydrocharitetum morsus-ranae;Thelypteridi-Phragmitetum;Cicuto-Caricetum pseudocyperi;Acoretum calami;Ceratophylletum demersi;Potametum lucentis。前三个是主导联想。淹没植被的存在似乎证实了所采取的保护和恢复措施的积极影响。叶绿素-a和总氮浓度显著降低,总悬浮物逐渐减少,透明度增加,证实了这种改善。
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引用次数: 11
Intraspecific morphological variation between cultured and wild Clarias gariepinus (Burchell) (Clariidae, Siluriformes) 养殖与野生克拉马尾的种内形态差异(克拉马尾科,志留目)
Q3 Agricultural and Biological Sciences Pub Date : 2015-03-01 DOI: 10.1515/aopf-2015-0006
S. G. Solomon, V. T. Okomoda, Abel I. Ogbenyikwu
Abstract This study was designed to evaluate morphological differences between cultured and wild African catfish, Clarias gariepinus (Burchell). Fish samples were collected from the lower Benue River (axis in Makurdi), while cultured fish were obtained from the University of Agriculture Makurdi research farm. The results revealed significant sex-related variation in the fish from different environments. Significant differences were observed in all morphometric parameters measured and in three of the five meristic counts recorded. Discriminant analysis and cluster analysis of morphometric parameters showed a high divergence among the populations, hence the tested fish samples were grouped into respective environments by sex. The meristic count, however, overlapped broadly showing no divergence among the populations. The morphometric differences between the cultured and wild African catfish could have been linked to genetic differences or environmental factors or a combination of both factors.
摘要本研究旨在评价养殖和野生非洲鲶鱼Clarias gariepinus (Burchell)的形态差异。鱼类样本采集自贝努埃河下游(马库尔迪中轴线),养殖鱼类采集自马库尔迪农业大学研究农场。结果显示,来自不同环境的鱼存在显著的性别差异。在所有测量的形态计量参数和记录的5个分生计数中的3个中观察到显著差异。形态计量学参数的判别分析和聚类分析表明,种群间存在较大差异,因此将测试鱼样本按性别划分到各自的环境中。然而,分生计数有很大的重叠,表明种群之间没有分化。养殖和野生非洲鲶鱼之间的形态差异可能与遗传差异或环境因素有关,也可能与两种因素的结合有关。
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引用次数: 45
Administrative, legal, natural, and socioeconomic factors that impede the functioning and development of inland fisheries in Poland 阻碍波兰内陆渔业运作和发展的行政、法律、自然和社会经济因素
Q3 Agricultural and Biological Sciences Pub Date : 2014-12-01 DOI: 10.2478/aopf-2014-0030
M. Mickiewicz, A. Wołos
Abstract The results of a questionnaire survey of the managers of fisheries enterprises entitled to operate in fisheries zones are presented. The aim of the study was to determine the degree to which selected factors have a negative impact on the functioning and development of fisheries. The impediment posed by the different factors was determined with a rating scale. The factors that posed impediments were ranked, from greatest to smallest, as follows: cormorant predation on ichthyofauna; commercial and recreational fisheries poaching; national and local administrations (e.g., flawed laws, bureaucracy, incompetence, etc.); unenforced regulations and laws or the lack of them; excessive leasing fees; water eutrophication and pollution; development of recreation linked with the aquatic environment; eco-terrorism, other fees payable to the state in addition to leasing fees; difficulties with selling fish; recreational fisheries pressure.
摘要本文介绍了对渔区经营权渔业企业经营者进行问卷调查的结果。这项研究的目的是确定选定的因素对渔业的功能和发展产生消极影响的程度。不同因素造成的障碍用一个评定量表来确定。构成障碍的因素由大到小排列如下:鸬鹚捕食鱼栖动物;商业和休闲渔业偷猎;国家和地方行政机构(例如,有缺陷的法律、官僚主义、无能等);法规、法律未执行或缺乏执行的;租赁费用过高;水体富营养化和污染;发展与水环境有关的康乐活动;生态恐怖主义,除租赁费用外,向国家缴纳的其他费用;卖鱼困难;休闲渔业压力。
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引用次数: 2
Survival rate and growth in the wild of sea trout (Salmo trutta L.) fry obtained using frozen semen 用冷冻精液获得的野生海鳟(Salmo trutta L.)鱼苗的存活率和生长
Q3 Agricultural and Biological Sciences Pub Date : 2014-12-01 DOI: 10.2478/aopf-2014-0028
J. Domagała, K. Dziewulska, R. Czerniawski
Abstract The aim of the study was to assess the growth and development of sea trout, Salmo trutta L., fry obtained from frozen, thawed semen after the fish had been released into the wild and were feeding there. The semen was cryopreserved with either methanol (MeOH) or dimethyl sulfoxide (DMSO). Fresh eggs were collected and fertilized with the thawed semen. The control was eggs fertilized with fresh semen that had been kept on ice. The eggs were incubated and reared in a closed recirculating system. The fertilized eggs were counted for visible eye pigment. After rearing, three groups of fish were tagged and stocked into a stream. The fish aged 6 months were caught from the stream. The fish were measured, weighed, identified to which experimental variant they belonged, and their survival rate was estimated. No significant differences in survival rate, length, weight, or condition factor among the control, DMSO, and MeOH groups were noted during larval development in a closed recirculating system. These parameters were also similar in the control and experimental groups after the fish had grow in a natural stream. The use of frozen semen to fertilize fresh sea trout eggs resulted in a normal incubation process, larval stage, and regular fry growth and survival, both under laboratory conditions, and, most importantly, in the wild. The results indicate it is possible to use frozen semen for fry production and the restoration of wild fish populations.
摘要本研究的目的是评估海鳟(Salmo trutta L.)在放归野外并在野外饲养后,通过冷冻、解冻精液获得的鱼苗的生长发育情况。精液用甲醇(MeOH)或二甲基亚砜(DMSO)冷冻保存。收集新鲜的卵子并用解冻的精液受精。对照组是用冰冻的新鲜精液受精的卵子。鸡蛋在一个封闭的循环系统中孵化和饲养。计算受精卵的可见眼色素。饲养后,三组鱼被贴上标签并放入溪流中。这条6个月大的鱼是从小溪里钓上来的。研究人员对这些鱼进行了测量、称重,确定了它们属于哪个实验变种,并估计了它们的存活率。在封闭循环系统的幼虫发育过程中,对照组、DMSO和MeOH组之间的存活率、长度、重量或条件因子均无显著差异。在鱼在自然溪流中生长后,这些参数在对照组和实验组中也相似。使用冷冻精液使新鲜的海鳟卵受精,无论是在实验室条件下,还是最重要的是在野外条件下,都能实现正常的孵化过程、幼虫阶段和正常的鱼苗生长和存活。结果表明,冷冻精液可用于鱼苗生产和野生鱼类种群恢复。
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引用次数: 3
Determining the spring biomass peak of the smallest plankters in selected vendace lakes in Western Pomerania 在西波美拉尼亚选定的vendace湖泊中测定最小浮游生物春季生物量峰值
Q3 Agricultural and Biological Sciences Pub Date : 2014-12-01 DOI: 10.2478/aopf-2014-0031
J. Domagała, Słukasz Ługocki, R. Czerniawski, M. Pilecka-Rapacz
Abstract The aim of this study was to determine the prevalence peak of the smallest plankters that are an adequate food resource for vendace (Coregonus albula L.) larvae. This goal was accomplished by determining weekly changes in zooplankton biomass, especially rotifers and nauplii, against changes in basic physicochemical parameters of lake waters. As the results of this paper show, the highest abundance of the smallest plankters, rotifers and nauplii, occurs at a particular temperature for a period of two weeks. The most important variable that determined the peak of small plankter biomass is temperature. Thus, the period of stocking lakes with vendace larvae should be synchronized with this temperature and the coinciding biomass peak of rotifers and nauplii, which are the basic food of vendace larvae.
摘要本研究的目的是确定为捕食者提供充足食物资源的最小浮游生物(Coregonus albula L.)幼虫的流行高峰。这一目标是通过测定浮游动物生物量的每周变化,特别是轮虫和nauplii,以及湖水基本物理化学参数的变化来实现的。正如这篇论文的结果所显示的那样,最小的浮游生物,轮虫和nauplii的丰度最高,发生在特定温度下的两周内。决定浮游生物生物量峰值的最重要变量是温度。因此,旺达丝幼虫的放养期应与该温度同步,并与作为旺达丝幼虫基本食物的轮虫和鹦鹉螺的生物量高峰重合。
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引用次数: 0
期刊
Archives of Polish Fisheries
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