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Bioremediation Ability of the Local Isolate Enterobacter cloacae from Disposal Site 处理场局部分离阴沟肠杆菌的生物修复能力
IF 0.6 Pub Date : 2022-04-10 DOI: 10.14500/aro.10948
H. A. Muhammad, H. Subhi, Khalid N. Sediq
Illegal dumping is a serious problem that needs to be addressed immediately to preserve human health and the environment as if the pollution that arises from it reaches the groundwater, complications of the remediation processes will increase. To decontaminate the organic and inorganic components, bioremediation seems to be the most environmentally friendly and economically viable technique without further treatment as reported by many studies. In this investigation, samples were taken from the soil of the main dumping area in Koysinjaq in Kurdistan Region of Iraq to determine the most potent bacteria to remediate the existed pollutants. The existence of non-essential minerals and organic compounds in the soil sample was detected using X-ray fluorescence device, and ethane and 1,2-dichloroethane solvents separating technique, respectively. Then, from the same samples, three different naturally occurring bacteria were isolated and cultured under optimized conditions then stimulated for a good result. Finally, spectrophotometer was set at wavelength of 600 nm and used to detect the heaviest growth of bacteria after incubating the cultured bacteria on a mineral salt broth medium with the extracted pollutants at pH 7.0 overnight at 32°C. Based on the highest absorbance, the most effective type of bacteria (Enterobacter cloacae) was chosen among others to remediate the organic components in which approximately 90% of them are plastics, medical waste, municipal waste, electrical items, and hydrocarbons, and some heavy metals, for instance aluminum and lead, which were found in the soil.
非法倾倒是一个需要立即处理的严重问题,以保护人类健康和环境。如果由此产生的污染达到地下水,补救过程的复杂性将增加。许多研究表明,生物修复技术是对有机和无机成分进行净化的最环保和经济可行的技术,无需进一步处理。在这项调查中,从伊拉克库尔德斯坦地区科伊辛克主要倾倒区的土壤中取样,以确定修复现有污染物的最有效细菌。采用x射线荧光仪和乙烷、1,2-二氯乙烷溶剂分离技术分别检测土壤样品中非必需矿物质和有机化合物的存在。然后,从相同的样品中分离出三种不同的天然细菌,并在优化的条件下进行培养,然后进行刺激以获得良好的结果。最后,分光光度计设置在600 nm波长,将培养的细菌与提取的污染物在pH 7.0的无机盐肉汁培养基中培养,在32℃下过夜,检测细菌生长最重。根据最高吸光度,在其他细菌中选择了最有效的细菌类型(阴沟肠杆菌)来修复有机成分,其中约90%是塑料、医疗废物、城市废物、电气产品和碳氢化合物,以及在土壤中发现的一些重金属,例如铝和铅。
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引用次数: 0
Suitability of the Carbonate Rocks of the Bekhme Formation Exposed in Shakrook Anticline, Iraqi Kurdistan region, for Cement Industry 伊拉克库尔德斯坦地区Shakrook背斜Bekhme组碳酸盐岩在水泥工业中的适用性
IF 0.6 Pub Date : 2022-03-25 DOI: 10.14500/aro.10907
Mohammed J. Hamwandy, R. K. Ibrahim, V. Sissakian
The Bekhme Formation forms almost the bulk of the Shakrook anticline, especially the limbs. The current research deals with studying the exposed beds within the Bekhme Formation at the Shakrook anticline to check the suitability of the exposed rocks at the northeastern limb of the anticline for the cement industry. Twenty rock samples from a section which lies along a deeply cut valley that crosses the northeastern limb of the Shakrook anticline within the Bekhme Formation were collected. The channel sampling method was applied; therefore, each sample represents the concerned sampling interval and to be representative for the thickness of the sampled interval. The total thickness of the sampled section is 110 m with a covered interval of 15 m, totaling to 125 m. The collected 20 samples were prepared at the laboratory of the Koya University and were subjected to XRF test at the Tarbiat Modares University, Iran, to indicate the concentration of the main oxides (CaO, MgO, Al2O3, Fe2O3, Na2O, K2O, and SO3), and Cl and L.O.I. The indicated concentrations at each sample, from both universities, were compared and were found to be almost coinciding. The average concentrations at each sample were changed to weighted averages and the results were compared with the Iraqi standards for cement industry. The results revealed that the sampled rocks are excellent for cement production.
贝克梅组几乎构成了沙克鲁克背斜的大部分,尤其是其分支。目前的研究工作是研究Shakrook背斜Bekhme组的裸露层,以检查背斜东北缘裸露岩石是否适合水泥工业。从贝克梅组内横跨Shakrook背斜东北翼的一个深切山谷的剖面中收集了20个岩石样本。采用通道采样法;因此,每个样本代表相关的采样区间,并代表采样区间的厚度。采样剖面总厚度为110 m,覆盖间距为15 m,总厚度为125 m。收集的20个样品在Koya大学实验室制备,并在伊朗Tarbiat Modares大学进行XRF测试,以指示主要氧化物(CaO, MgO, Al2O3, Fe2O3, Na2O, K2O和SO3)以及Cl和L.O.I.的浓度。来自两所大学的每个样品的指示浓度进行了比较,发现几乎一致。将每个样品的平均浓度改为加权平均值,并将结果与伊拉克水泥工业标准进行比较。结果表明,该样品具有良好的水泥生产性能。
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引用次数: 0
Detecting Deepfakes with Deep Learning and Gabor Filters 用深度学习和Gabor过滤器检测深度伪造
IF 0.6 Pub Date : 2022-03-18 DOI: 10.14500/aro.10917
Wildan J. Jameel, S. Kadhem, A. Abbas
The proliferation of many editing programs based on artificial intelligence techniques has contributed to the emergence of deepfake technology. Deepfakes are committed to fabricating and falsifying facts by making a person do actions or say words that he never did or said. So that developing an algorithm for deepfakes detection is very important to discriminate real from fake media. Convolutional neural networks (CNNs) are among the most complex classifiers, but choosing the nature of the data fed to these networks is extremely important. For this reason, we capture fine texture details of input data frames using 16 Gabor filters indifferent directions and then feed them to a binary CNN classifier instead of using the red-green-blue color information. The purpose of this paper is to give the reader a deeper view of (1) enhancing the efficiency of distinguishing fake facial images from real facial images by developing a novel model based on deep learning and Gabor filters and (2) how deep learning (CNN) if combined with forensic tools (Gabor filters) contributed to the detection of deepfakes. Our experiment shows that the training accuracy reaches about 98.06% and 97.50% validation. Likened to the state-of-the-art methods, the proposed model has higher efficiency.
许多基于人工智能技术的编辑程序的激增促成了深度造假技术的出现。Deepfakes致力于通过让一个人做他从未做过或说过的话来制造和伪造事实。因此,开发一种深度造假检测算法对于区分真假媒体是非常重要的。卷积神经网络(cnn)是最复杂的分类器之一,但选择提供给这些网络的数据的性质非常重要。出于这个原因,我们使用16个Gabor滤波器捕获输入数据帧的精细纹理细节,然后将它们提供给二进制CNN分类器,而不是使用红-绿-蓝颜色信息。本文的目的是让读者更深入地了解(1)通过开发基于深度学习和Gabor滤波器的新模型来提高区分假面部图像和真实面部图像的效率,以及(2)如果将深度学习(CNN)与取证工具(Gabor滤波器)相结合,如何有助于检测深度伪造。实验表明,训练准确率达到98.06%左右,验证率达到97.50%。与目前最先进的方法相比,该模型具有更高的效率。
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引用次数: 1
Application of Experimental Design Methodology for Adsorption of Brilliant Blue onto Amberlite XAD-4/Agaricus campestris as a New Biocomposite Adsorbent 新型生物复合吸附剂Amberlite XAD-4/Agaricus campestris吸附亮蓝的实验设计方法
IF 0.6 Pub Date : 2022-02-25 DOI: 10.14500/aro.10903
Ahmed A. Ahmed, V. Yönten
This research presents a new biocomposite adsorbents using response surface methodology (RSM) to find the best conditions for highest adsorption of Brilliant Blue G250 (BBG) from aqueous solution by Amberlite XAD-4/Agaricus campestris. The most effective parameters are determined by Plackett–Burman design (PBD) with specific ranges initial dye concentration (5–150 mg.L-1), temperature (20–50°C), contact time (5–100 min), pH (3–11), shaking speed (150–300 rpm), sample volume (5–75 mL), and adsorbent dosage (0.05–0.6 g). Then, in the second step, the optimum condition of effective factors is predicted using steepest ascent design. Finally, optimal medium conditions of effective parameters with central composite design are located. According to RSM, the best adsorbent amount, contact time, initial dye concentration, and sample volume for maximum removal% of BBG (96.72%) are 0.38 g, 60.78 min, 107.13 mg.L-1, and 28.6 mL, respectively. The adsorption of brilliant blue is approved by scanning electron microscopy. Under optimum conditions, it is concluded that XAD4/A. campestr is biocomposite is a suitable adsorbent for removing BBG from aqueous solution.
采用响应面法(RSM)研究了Amberlite XAD-4/Agaricus campestris在水溶液中吸附Brilliant Blue G250 (BBG)的最佳条件。通过Plackett-Burman设计(PBD)确定了最有效的参数范围,即初始染料浓度(5-150 mg.L-1)、温度(20-50°C)、接触时间(5-100 min)、pH(3-11)、震动速度(150-300 rpm)、进样量(5-75 mL)和吸附剂用量(0.05-0.6 g)。然后,在第二步中,采用最急剧上升设计预测了有效因素的最佳条件。最后,通过中心组合设计确定了有效参数的最佳介质条件。RSM实验结果表明,吸附量、接触时间、初始染料浓度和样品体积分别为0.38 g、60.78 min、107.13 mg,对BBG的去除率达到96.72%。L-1和28.6 mL。扫描电镜证实了其对亮蓝色的吸附作用。在最佳条件下,XAD4/A。生物复合材料是一种去除水溶液中BBG的合适吸附剂。
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引用次数: 0
Cement Percent Effect on the Shear and Interface Strength of Remolded Cement Treated Sand 水泥掺量对水泥处理砂抗剪强度和界面强度的影响
IF 0.6 Pub Date : 2022-02-19 DOI: 10.14500/aro.10805
Zahraa N. Rashied
This research aims to simulate the behavior of remolded cement treated poorly graded sand in term of shear and interface strength using the direct shear test. Different percentages of cement up to 15% by weight are added to the soil samples. Compacted cement treated soil samples are prepared at the optimum moisture content and left for 28 days in the humidity room then distributed to use them for the remolding samples preparation. The shear strength parameters for both cases soil to soil interface and concrete to soil interface are predicted, where the results show that the interface strength parameters are higher than the shear strength parameters of the remolded soil samples. The increase in cement percent increases the cohesion (C) of the treated soil, whereas the interface cohesion (Cint.) has a maximum value at 10% of added cement, and the maximum percent between cohesion (Cint.) and soil cohesion (C) is of 76.2% at 0% added cement. Moreover, the results show an increase in the interface angle of friction (δ) and a decrease in the angle of friction (φ) as the percent of cement increases. The maximum percent between interface angle (δ) and angle of friction (φ) is 63.5% at 15% of the added cement. The hardened cement in the remolded case adheres to sand grains and works as soil grains with different sizes that lead to changes in the shear properties of the soil.
本研究旨在通过直剪试验模拟改造水泥处理差级配砂的抗剪和界面强度行为。在土壤样品中加入不同比例的水泥,最高可达重量的15%。在最佳含水率下制备水泥处理后的土样,在湿室中放置28天,然后分配用于重塑样的制备。对两种情况下土-土界面和混凝土-土界面的抗剪强度参数进行了预测,结果表明,界面强度参数高于重塑土样品的抗剪强度参数。水泥掺量的增加增加了处理土的黏结力(C),而界面黏结力(Cint.)在水泥掺量为10%时达到最大值,在水泥掺量为0%时,黏结力(Cint.)与土壤黏结力(C)之间的最大百分比为76.2%。随着水泥掺量的增加,界面摩擦角δ增大,摩擦角φ减小。当水泥掺量为15%时,界面角δ与摩擦角φ之间的最大百分比为63.5%。在重塑情况下,硬化的水泥附着在砂粒上,作为不同大小的土粒,导致土的剪切特性发生变化。
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引用次数: 0
Wound Healing Properties and Structural Analysis of Four Geographical Areas’ Natural Clays 四个地理区域天然粘土的伤口愈合特性及结构分析
IF 0.6 Pub Date : 2022-02-15 DOI: 10.14500/aro.10889
Zahra A. Amin
Clays are fine particle materials that harden after drying. The difference in their structure is the key to their efficacy and their subsequent application. The current study aims to evaluate the wound healing property of four countries (C1:Iraq, C2:Turkey, C3:Azerbaijan and C4:Russia) clay samples by excision model using Sprague dawley rats also the chemical analysis of the samples was performed using X-ray diffraction (XRD) and X-ray Fluorescence (XRF) methods. Results revealed that the best wound healing activities were given by C1, C3, C4 and C2 respectively with healing percentages (76%, 71%, 62%, and 60%), respectively. XRD results revealed the presence of Calcium carbonate and CalciumMagnesium carbonate in C1, Dolomite and Calcium-Magnesium carbonate in C2, Cobalt Tantalum Sulfide in C3, Finally Quartz and Silicon Oxide in C4. On the other hand, XRF analysis showed the appearance of different major and trace elements with different quantities in each clay type. We conclude that different countries clays enclose wound healing property with diverse ranges and this diversity is due to their chemical and mineral structures.
粘土是干燥后变硬的细颗粒材料。它们的结构差异是影响其有效性和后续应用的关键。本研究采用大鼠切除模型对4个国家(C1:伊拉克、C2:土耳其、C3:阿塞拜疆和C4:俄罗斯)粘土样品的伤口愈合性能进行了评价,并用x射线衍射(XRD)和x射线荧光(XRF)方法对样品进行了化学分析。结果显示,C1、C3、C4和C2的创面愈合活性最佳,愈合率分别为76%、71%、62%和60%。XRD结果表明,C1中存在碳酸钙和碳酸钙镁,C2中存在白云石和碳酸钙镁,C3中存在硫化钽钴,C4中存在石英和氧化硅。另一方面,XRF分析表明,每种粘土类型中出现了不同数量的主微量元素。我们得出结论,不同国家的粘土封闭伤口愈合性能有不同的范围,这种多样性是由于它们的化学和矿物结构。
{"title":"Wound Healing Properties and Structural Analysis of Four Geographical Areas’ Natural Clays","authors":"Zahra A. Amin","doi":"10.14500/aro.10889","DOIUrl":"https://doi.org/10.14500/aro.10889","url":null,"abstract":"Clays are fine particle materials that harden after drying. The difference in their structure is the key to their efficacy and their subsequent application. The current study aims to evaluate the wound healing property of four countries (C1:Iraq, C2:Turkey, C3:Azerbaijan and C4:Russia) clay samples by excision model using Sprague dawley rats also the chemical analysis of the samples was performed using X-ray diffraction (XRD) and X-ray Fluorescence (XRF) methods. Results revealed that the best wound healing activities were given by C1, C3, C4 and C2 respectively with healing percentages (76%, 71%, 62%, and 60%), respectively. XRD results revealed the presence of Calcium carbonate and CalciumMagnesium carbonate in C1, Dolomite and Calcium-Magnesium carbonate in C2, Cobalt Tantalum Sulfide in C3, Finally Quartz and Silicon Oxide in C4. On the other hand, XRF analysis showed the appearance of different major and trace elements with different quantities in each clay type. We conclude that different countries clays enclose wound healing property with diverse ranges and this diversity is due to their chemical and mineral structures.","PeriodicalId":8398,"journal":{"name":"ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90286498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Contact Geometrical Study for Top Emitting 980 nm InGaAs/GaAsP Vertical-Cavity Surface Emitting Lasers 顶发射980 nm InGaAs/GaAsP垂直腔面发射激光器的接触几何研究
IF 0.6 Pub Date : 2021-12-26 DOI: 10.14500/aro.10845
F. Chaqmaqchee
Geometrical contacts of a double mesa structure with 16 rows ×15 columns arrays of top emitting GaAs based 980 nm vertical cavity surface emitting lasers (VCSELs) are fabricated and characterized. In this paper, 5 strained In0.22Ga0.78As/Ga0.9AsP0.1 quantum wells (QWs) within λ/2 thick cavity have been employed. The top and the bottom epitaxially grown mirrors are based on the linear graded Al0.9Ga0.1As/GaAs distributed Bragg reflectors (DBRs) with 20.5 and 37 periods, respectively. Static parameters including threshold currents, rollover currents, maximum optical output power and wall-plug efficiency are extracted from light out power-current-voltage (LIV) of VCSELs with fixed oxide aperture diameter of ∅~ 6 μm and various mesa2 diameters. In addition, spectral emission for 980 nm VCSELs of oxide aperture between ∅~ 6 and 19 μm and with fixed ∅~ 6 μm and different bias currents are analyzed. The highest optical output power of around 33 dBm is observed at bias current of 0.8 mA for short−reach optical interconnect applications.
制备并表征了顶发射GaAs基980 nm垂直腔面发射激光器(VCSELs)的16列×15柱双台面结构的几何接触。本文利用λ/2厚腔内的5个应变In0.22Ga0.78As/Ga0.9AsP0.1量子阱(QWs)。顶部和底部外延生长的反射镜分别基于20.5和37周期的线性梯度Al0.9Ga0.1As/GaAs分布式Bragg反射镜(dbr)。从固定氧化物孔径为∅~ 6 μm、不同介面直径的vcsel的出光功率-电流电压(LIV)中提取出阈值电流、侧翻电流、最大光输出功率和插墙效率等静态参数。对氧化物孔径在∅~ 6 ~ 19 μm之间、固定∅~ 6 μm、不同偏置电流的980 nm vcsel的光谱发射进行了分析。当偏置电流为0.8 mA时,可获得约33 dBm的最高光输出功率,用于短距离光互连应用。
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引用次数: 1
Flood Modeling on Koya Catchment Area Using Hyfran, Web Map Service, and HEC-RAS Software 基于Hyfran、Web Map Service和HEC-RAS软件的Koya流域洪水模型研究
IF 0.6 Pub Date : 2021-12-25 DOI: 10.14500/aro.10824
A. Dawood, Dana K. Mawlood, N. Al‐Ansari
In this research, The boundaries of the Koya catchment area have been delineated, and valley paths in the region were drawn by using the Water Modeling System (WMS) software, Analysis of the morphometric information indicated that the morphometric characteristics of watersheds contribute to the floods. The average surface runoff depth depends on the curve number values that are determined based on the types of soil cover and soil class according to Harmonized World Soil Database HWSD software that indicates the soil class in the study area are Group B silt loam,  The results obtained also show that the potential for surface runoff varies with land use and soil characteristics. Also, the value of the curve number (CN) was determined to be 71. The hydrological modeling was performed by the HEC-HMS program that simulates the process of rainfall to runoff using the SCS curve number model. A flood hydrograph was constructed at the catchment area outlet and the floodplain delineation was verified by the HEC-RAS software. The results indicated that the 100-year return period flood could Reach critical areas such as the urban area, agricultural area, residential areas. the results of this study indicate that there are suitable sites in the catchment areas for constructing small dams and ponds for water harvesting. 
本研究利用WMS软件对高崖流域进行了边界划分,并绘制了流域内的流域路径,对流域的形态特征信息进行了分析,发现流域的形态特征对洪水的发生有一定的影响。平均地表径流深度取决于基于土壤覆盖类型和土壤类别的曲线数值,该曲线数值表明研究区土壤类别为B类粉砂质壤土,所得结果还表明地表径流潜力随土地利用方式和土壤特征而变化。同时,曲线数(CN)的值确定为71。水文建模由HEC-HMS程序执行,该程序使用SCS曲线数模型模拟降雨到径流的过程。在汇水区出水口处绘制了洪水线图,并用HEC-RAS软件对河漫滩圈定结果进行了验证。结果表明,百年一遇的洪涝灾害可能会到达城市、农业区、居住区等关键区域。研究结果表明,在集水区有适宜的场地建设小型水坝和集水塘。
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引用次数: 0
A Review of the Development and Applicability of Double-Skin Facades in Hot Climates 热气候条件下双层幕墙的发展与应用综述
IF 0.6 Pub Date : 2021-12-20 DOI: 10.14500/aro.10859
Aare N. Abtar
A double-skin façade (DSF) is a multilayered skin that was initially designed for the cold climate of European countries with noticeable success. Since then, countries in different climates started looking into the possibility of adopting DSF through the use of computer simulations to avoid any unforeseen problems in real life. This study aims to look into the possibility and level of success in using DSF in the Middle East’s hot-arid climate, making it a challenge compared to European countries. The study utilized a quantitative investigative approach in analyzing the results of some studies done in different countries in the Middle East. After looking into the results from different papers, careful considerations have to be made for the building due to its location and microclimate todetermine specific parameters (e.g., combination of transparent and opaque materials, proportion between floor level and screen height, orientation and cavity depth), these would lead to a significant impact on reducing a building’s cooling loads and energy efficiency. It is worth noting that mechanical ventilation (e.g., supplying all return cool air from internal spaces into the DSF cavity or integrating the building’s heating, ventilation, and air-conditioning system with the façade) is necessary for DSF in hot climates to cool and maintain its cavity’s temperature to function properly. However, this increases cooling loads, energy consumption, and running costs of the building which architects have to consider to determine the most efficient and economical solution in material and equipment.  
双层皮肤farade (DSF)是一种多层皮肤,最初是为欧洲国家的寒冷气候而设计的,取得了显著的成功。从那时起,不同气候的国家开始通过使用计算机模拟研究采用DSF的可能性,以避免在现实生活中出现任何不可预见的问题。本研究旨在探讨在中东炎热干旱气候中使用DSF的可能性和成功程度,与欧洲国家相比,这是一个挑战。本研究采用定量调查的方法分析了在中东不同国家进行的一些研究的结果。在研究了不同论文的结果后,必须仔细考虑建筑物的位置和微气候,以确定具体参数(例如,透明和不透明材料的组合,地板水平和屏幕高度之间的比例,朝向和腔深),这些将对减少建筑物的冷负荷和能源效率产生重大影响。值得注意的是,机械通风(例如,将所有从内部空间返回的冷空气供应到DSF腔内或将建筑物的采暖,通风和空调系统与立面集成)对于炎热气候下的DSF来说是必要的,以冷却并保持其腔内的温度以正常运行。然而,这增加了建筑的冷却负荷、能源消耗和运行成本,建筑师必须考虑这些因素,以确定最有效和最经济的材料和设备解决方案。
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引用次数: 1
Chemical Composition and Cytotoxic Activity of Pistacia atlantica var. kurdica Fruits 黄连木果实化学成分及细胞毒活性研究
IF 0.6 Pub Date : 2021-12-15 DOI: 10.14500/aro.10868
Raad A. Kaskoos, Javed Ahamad, Subasini Uthirapathy
Pistacia atlantica var. kurdica (Anacardiaceae) is a major subspecies found in the Kurdistan region of Iraq and has several beneficial bioactivities such as antioxidant, antibacterial, and antiviral. P. atlantica growing in the Kurdistan region is not yet studied phytochemically and pharmacologically. Hence, the goal of the present study is to characterize chemical compounds present in ethanolic extract of P. atlantica fruits by gas chromatography–mass spectroscopy (GC–MS) and to evaluate cytotoxic activity using A549 (human lung cancer) cell lines by (3-4,5 dimethylthiazol-2yl2,5-diphenyl tetrazolium bromide) assay. The GC–MS analysis shows the presence of 33 chemical compounds which constitute about 99.95% of total compounds, and isospathulenol (14.63%), spathulenol (13.45%), α-terpineol (13.28%), limonene (10.92%), terpinolene (10.89%), β-myrcene (6.92%), ethyl pentadecanoate (6.15%), β-pinene (4.98%), and caryophyllene oxide (4.01%) were found as major chemical compounds. P. atlantica inhibits cell proliferation in A549 cell lines in a time (24 h) and dose-dependent manner (0.5–500 µg/mL). After 24 h of treatment with P. atlantica, the cell viability of A549 cell lines ranged from 93.01 ± 5.24 to 57.69 ± 4.15% for concentrations of 0.5–500 µg/mL, respectively. This study expands the knowledge of the chemical composition of P. atlantica fruits and provides scientific evidence for its possible use as an anticancer medicine. The substantial anticancer activity of P. atlantica fruits may be due to the presence of isospathulenol, spathulenol, α-terpineol, limonene, terpinolene, β-myrcene, ethyl pentadecanoate, β-pinene, and caryophyllene oxide.
黄连木是伊拉克库尔德斯坦地区的一种主要亚种,具有抗氧化、抗菌、抗病毒等多种有益生物活性。生长在库尔德斯坦地区的大西洋蕨尚未进行植物化学和药理学研究。因此,本研究的目的是采用气相色谱-质谱(GC-MS)技术对大西洋青果乙醇提取物中的化合物进行表征,并采用(3-4,5二甲基噻唑-2yl2,5-二苯基溴化四氮唑)测定法对A549(人肺癌)细胞株的细胞毒活性进行评价。GC-MS分析表明,共鉴定出33种化合物,占总化合物的99.95%,其中以异spathulenol(14.63%)、spathulenol(13.45%)、α-松油醇(13.28%)、柠檬烯(10.92%)、松油烯(10.89%)、β-月桂烯(6.92%)、戊酸乙酯(6.15%)、β-蒎烯(4.98%)、石竹烯氧化物(4.01%)为主要化合物。P. atlantica对A549细胞系的细胞增殖具有时间依赖性(24 h)和剂量依赖性(0.5 ~ 500µg/mL)。在0.5 ~ 500µg/mL浓度下,大西洋青霉作用24 h后,A549细胞株的细胞活力为93.01±5.24 ~ 57.69±4.15%。本研究扩大了对大西洋树果实化学成分的认识,为其可能作为抗癌药物提供了科学依据。大西洋木果实具有显著的抗癌活性可能是由于其含有异spathulenol、spathulenol、α-松油醇、柠檬烯、terpinolene、β-月桂烯、戊酸乙酯、β-蒎烯和石竹烯氧化物。
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引用次数: 2
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