Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00040
Md. Abdul Sattar, M. S. Sree Lakshmi, D. S. Jyothi, M. Sudha, M. K. Siva Krishna
A new method was established for estimation of Tizanidine and Ibuprofen by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Tizanidine and Ibuprofen by using Intersil ODS C18 column (250×4.6mm) 5.0µm, flow rate was 1.0ml/min, mobile phase ratio was (40:60 v/v) Potassium di hydrogen Phosphate Buffer: Acetonitrile pH 3.2 (pH was adjusted with Ortho-phosphoric acid), detection wavelength was 269nm. The instrument used was Shimadzu HPLC, UV detector 2450, Spinchrom -software version-2. The method shows linearity between the concentration range of 0.6-1.4µg/ml for Tizanidine and 120-280 for Ibuprofen. The % recovery of Tizanidine and Ibuprofen were found to be in the range of 98.0% - 102.0%. As there was no interference due to mobile phase, the method was found to be specific. The method was robust as observed from insignificant variation in the results of analysis by changes in Flow rate and wavelength variation separately and analysis being performed by different analysts.
{"title":"Development and Validation of a HPLC Method for the Estimation of Tizanidine and Ibuprofen in Bulk and Dosage forms","authors":"Md. Abdul Sattar, M. S. Sree Lakshmi, D. S. Jyothi, M. Sudha, M. K. Siva Krishna","doi":"10.52711/2231-5675.2022.00040","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00040","url":null,"abstract":"A new method was established for estimation of Tizanidine and Ibuprofen by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Tizanidine and Ibuprofen by using Intersil ODS C18 column (250×4.6mm) 5.0µm, flow rate was 1.0ml/min, mobile phase ratio was (40:60 v/v) Potassium di hydrogen Phosphate Buffer: Acetonitrile pH 3.2 (pH was adjusted with Ortho-phosphoric acid), detection wavelength was 269nm. The instrument used was Shimadzu HPLC, UV detector 2450, Spinchrom -software version-2. The method shows linearity between the concentration range of 0.6-1.4µg/ml for Tizanidine and 120-280 for Ibuprofen. The % recovery of Tizanidine and Ibuprofen were found to be in the range of 98.0% - 102.0%. As there was no interference due to mobile phase, the method was found to be specific. The method was robust as observed from insignificant variation in the results of analysis by changes in Flow rate and wavelength variation separately and analysis being performed by different analysts.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"83 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73061839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00047
G. Shinde, K. Prasad, R. S. Jadhav, D. Vikhe
Monoclonal antibodies are antibodies that are identical because they were produced by one type of immune cell, all Human monoclonal antibody immunotherapy in clinical medicine has been an exciting prospect for some time, and increasing numbers of such antibodies have gradually become available. Targets and basic characterization of novel classes of bioactive agents, to the development of ‘designer’ or ‘smart’ materials which provide required excipient or carrier properties to achieve modulated and targeted drug delivery. Drug particle engineering, or crystal engineering, provides an additional dimension to drug delivery and targeting. Traditional methods of particle formation, crystallization. Antibodies can be heterogeneous with respect to size, charge, antigen specificity, and affinity. These factors may be significant when antibodies are used as a drug delivery system. Drug targeting and delivery using antibodies has been most useful in the field of chemotherapy because this is an area of research in which there is the greatest need for target-site specificity. Antibodies have been found to have many applications in the management of human carcinomas, including colorectal, gastric, ovarian, endometrial, breast, lung.
{"title":"Monoclonal Antibodies as a Site Specific Particulate Drug Delivery System: A Review","authors":"G. Shinde, K. Prasad, R. S. Jadhav, D. Vikhe","doi":"10.52711/2231-5675.2022.00047","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00047","url":null,"abstract":"Monoclonal antibodies are antibodies that are identical because they were produced by one type of immune cell, all Human monoclonal antibody immunotherapy in clinical medicine has been an exciting prospect for some time, and increasing numbers of such antibodies have gradually become available. Targets and basic characterization of novel classes of bioactive agents, to the development of ‘designer’ or ‘smart’ materials which provide required excipient or carrier properties to achieve modulated and targeted drug delivery. Drug particle engineering, or crystal engineering, provides an additional dimension to drug delivery and targeting. Traditional methods of particle formation, crystallization. Antibodies can be heterogeneous with respect to size, charge, antigen specificity, and affinity. These factors may be significant when antibodies are used as a drug delivery system. Drug targeting and delivery using antibodies has been most useful in the field of chemotherapy because this is an area of research in which there is the greatest need for target-site specificity. Antibodies have been found to have many applications in the management of human carcinomas, including colorectal, gastric, ovarian, endometrial, breast, lung.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81953473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00036
S. Venkatesh, V. Laxmi, Rajeswari Chiluka, Y. Padmavathi
Piperine is an alkaloid obtained from Piper nigrum Linn., is known to modify the biotransformation of the drugs by inhibiting Cytochrome P450 forms. Piperine inhibits the bio-transformation and metabolism of metformin leading to higher levels of drug in systemic circulation, thus increases the bioavailabilty when co-administered with metformin. In the present study a simple, accurate, precise, economical procedures, entailing neither irksome sample treatment nor tedious extraction process a combined tablet dosage form of metformin and piperine have been developed for the simultaneous estimation of piperine and metformin using UV- Spectrophotometry method. The developed method was based on employing simultaneous equation method for analysis of both the drugs. Metformin and piperine have shown absorbance maxima at 237nm and 342nm respectively, in methanol and the linearity was obeyed over the concentration range of 2-10μg/ml and 2-10 μg/ml respectively for metformin and piperine. The validation parameters like precision, accuracy, limit of quantitation (LOQ), limit of detection (LOD), were determined according to ICH guidelines. All the results were found to be within the limits.
{"title":"Development of UV- Spectrophotometric Method for Simultaneous Estimation of Metformin and Piperine in Combined Tablet Dosage Form","authors":"S. Venkatesh, V. Laxmi, Rajeswari Chiluka, Y. Padmavathi","doi":"10.52711/2231-5675.2022.00036","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00036","url":null,"abstract":"Piperine is an alkaloid obtained from Piper nigrum Linn., is known to modify the biotransformation of the drugs by inhibiting Cytochrome P450 forms. Piperine inhibits the bio-transformation and metabolism of metformin leading to higher levels of drug in systemic circulation, thus increases the bioavailabilty when co-administered with metformin. In the present study a simple, accurate, precise, economical procedures, entailing neither irksome sample treatment nor tedious extraction process a combined tablet dosage form of metformin and piperine have been developed for the simultaneous estimation of piperine and metformin using UV- Spectrophotometry method. The developed method was based on employing simultaneous equation method for analysis of both the drugs. Metformin and piperine have shown absorbance maxima at 237nm and 342nm respectively, in methanol and the linearity was obeyed over the concentration range of 2-10μg/ml and 2-10 μg/ml respectively for metformin and piperine. The validation parameters like precision, accuracy, limit of quantitation (LOQ), limit of detection (LOD), were determined according to ICH guidelines. All the results were found to be within the limits.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88407907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00038
Anil K Agarwal, A. Yadav, C. P, Paromita Kuity, Jaya Mishra
Aim/Objective: The primary objective of this review is to describe the Evolution of current allopathic Anti-malarial Pharmacotherapy and understanding significance of Antimalarial drugs in Malaria Program/Public Health Program in India. Subject/Method: Review of Research Literature and Context-Content-Outcome Analysis of Antimalarial Drug Policy. Findings: Antimalarial drug list expanded extensively and the drug dose intensity is proportional to disease transmission in region/area; both the factors medicalizing the community significantly. The conceptual understanding of malaria remains within biomedicine domain evidenced by the fact that much of importance (over-reliance) given to least effective individual level technical interventions like drugs that too; without considering immunity and nutritional status of both individual and community. Indian Planners seems confused to differentiate (or ignoring) between Malaria’s behavior at Individual level and community level. Thus, the Malaria planning lacks ethical consideration for resource allocation in Public Health Planning evidenced by the fact that large resources allocated to least effective intervention that serves the profit making purpose of private sector pharma industry. Sufferings of the most impoverished household of community remain unaddressed in Health planning. Policy for Antimalarial Pharmacotherapy is unable to serve its primary purpose of relieving suffering of community, prevention of mortalities, reducing morbidities, slowing down the resistance, and reducing drug load in community. Conclusion: The investment in medications to tackle malaria as Public Health problem looks costly investments as it consumes heavy resources and its benefits/success claim is questionable. There is mismatch in planning and field reality as intervention in the form of drugs does not match the requirement as per epidemiological complexity. Health Planning is comprehensive exercise for any country. Ignoring voices from field/frontline workers prove detrimental for health planning. Every organization or individual involved in health planning would be having different opinions as per their interest but the final decision regarding resource allocation should consider the field reality following good public health ethics.
{"title":"Development of Antimalarial Pharmacotherapy and its importance in Malaria Treatment/Public Health Program","authors":"Anil K Agarwal, A. Yadav, C. P, Paromita Kuity, Jaya Mishra","doi":"10.52711/2231-5675.2022.00038","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00038","url":null,"abstract":"Aim/Objective: The primary objective of this review is to describe the Evolution of current allopathic Anti-malarial Pharmacotherapy and understanding significance of Antimalarial drugs in Malaria Program/Public Health Program in India. Subject/Method: Review of Research Literature and Context-Content-Outcome Analysis of Antimalarial Drug Policy. Findings: Antimalarial drug list expanded extensively and the drug dose intensity is proportional to disease transmission in region/area; both the factors medicalizing the community significantly. The conceptual understanding of malaria remains within biomedicine domain evidenced by the fact that much of importance (over-reliance) given to least effective individual level technical interventions like drugs that too; without considering immunity and nutritional status of both individual and community. Indian Planners seems confused to differentiate (or ignoring) between Malaria’s behavior at Individual level and community level. Thus, the Malaria planning lacks ethical consideration for resource allocation in Public Health Planning evidenced by the fact that large resources allocated to least effective intervention that serves the profit making purpose of private sector pharma industry. Sufferings of the most impoverished household of community remain unaddressed in Health planning. Policy for Antimalarial Pharmacotherapy is unable to serve its primary purpose of relieving suffering of community, prevention of mortalities, reducing morbidities, slowing down the resistance, and reducing drug load in community. Conclusion: The investment in medications to tackle malaria as Public Health problem looks costly investments as it consumes heavy resources and its benefits/success claim is questionable. There is mismatch in planning and field reality as intervention in the form of drugs does not match the requirement as per epidemiological complexity. Health Planning is comprehensive exercise for any country. Ignoring voices from field/frontline workers prove detrimental for health planning. Every organization or individual involved in health planning would be having different opinions as per their interest but the final decision regarding resource allocation should consider the field reality following good public health ethics.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80178804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00044
Ajay I. Patel, Jenish Rachhadiya, Purvi Vadariya, Amitkumar J. Vysh, Ashok B. Patel
Diabetes mellitus both type I and type II, is a dangerous and lifelong disorder marked by unusually high blood glucose levels caused by a failure of insulin synthesis or a loss in insulin sensitivity and function. Diabetes have become more common over time, and it is now considered one of the primary causes of high death and morbidity rates. Complications from diabetes can be avoided by regularly monitoring and keeping blood glucose levels within the normal range. Despite the fact that practically all commercially effective blood glucose monitoring devices are invasive, there is an urgent need to create non-invasive glucose monitoring (NGM) devices that would relieve diabetics' pain and suffering associated with repeated skin pricking for glucose testing. It also discusses the most common non-invasive glucose monitoring technologies as well as the most relevant devices. The technology name, the underlying physical principle, and the technological limitation in the human body. There are eleven technologies and five devices in all. Despite numerous fascinating and promising ideas and devices, the review concluded that a suitable solution to the non-invasive glucose monitoring problem still required more work.
{"title":"A Critical Review on Current Devloping Non-Invasive Glucose Monitoring Technologies and Devices","authors":"Ajay I. Patel, Jenish Rachhadiya, Purvi Vadariya, Amitkumar J. Vysh, Ashok B. Patel","doi":"10.52711/2231-5675.2022.00044","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00044","url":null,"abstract":"Diabetes mellitus both type I and type II, is a dangerous and lifelong disorder marked by unusually high blood glucose levels caused by a failure of insulin synthesis or a loss in insulin sensitivity and function. Diabetes have become more common over time, and it is now considered one of the primary causes of high death and morbidity rates. Complications from diabetes can be avoided by regularly monitoring and keeping blood glucose levels within the normal range. Despite the fact that practically all commercially effective blood glucose monitoring devices are invasive, there is an urgent need to create non-invasive glucose monitoring (NGM) devices that would relieve diabetics' pain and suffering associated with repeated skin pricking for glucose testing. It also discusses the most common non-invasive glucose monitoring technologies as well as the most relevant devices. The technology name, the underlying physical principle, and the technological limitation in the human body. There are eleven technologies and five devices in all. Despite numerous fascinating and promising ideas and devices, the review concluded that a suitable solution to the non-invasive glucose monitoring problem still required more work.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87884627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00039
Priya Barbude, M. Tawar, Prashant J. Burange
Type 2 diabetes mellitus (T2DM) is a disorder distinguished by resisting insulin effects and/or its reduced secretion leading to high blood sugar levels. It is fast becoming epidemic worldwide and is a major cause of death in the past years. Maintaining a correct blood sugar level is the primary target in the management of T2DM. Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation and sensitive-free direct a new, simple, precise, accurate, reproducible, and efficient UV spectrophotometric method is developed and validated for the simultaneous estimation of ternary mixture of metformin (MET), saxagliptin (SXG) and dapagliflozin (DGF) in both their bulk form and combined in tablet dosage form recently approved by FDA in 2019 to be used for treatment of Type 2 diabetes mellitus by simultaneous equation method. The solutions of standard and sample were prepared in methanol: water (80:20 v/v). The 𝜆max for MET, SXG, and DGF were 232.0, 212.0 and 272.0nm, respectively. Calibration curves are linear in the concentration ranges 10-50𝜇g/ml for MET, 1-5𝜇g/ml for SXG and 5-25𝜇g/ml for DGF, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.
{"title":"Method Development using a UV Visible Spectrophotometer for the Simultaneous Estimation of Metformin (MET), Saxagliptin (SXG), and Dapagliflozin (DGF) in Marketed Formulation","authors":"Priya Barbude, M. Tawar, Prashant J. Burange","doi":"10.52711/2231-5675.2022.00039","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00039","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is a disorder distinguished by resisting insulin effects and/or its reduced secretion leading to high blood sugar levels. It is fast becoming epidemic worldwide and is a major cause of death in the past years. Maintaining a correct blood sugar level is the primary target in the management of T2DM. Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation and sensitive-free direct a new, simple, precise, accurate, reproducible, and efficient UV spectrophotometric method is developed and validated for the simultaneous estimation of ternary mixture of metformin (MET), saxagliptin (SXG) and dapagliflozin (DGF) in both their bulk form and combined in tablet dosage form recently approved by FDA in 2019 to be used for treatment of Type 2 diabetes mellitus by simultaneous equation method. The solutions of standard and sample were prepared in methanol: water (80:20 v/v). The 𝜆max for MET, SXG, and DGF were 232.0, 212.0 and 272.0nm, respectively. Calibration curves are linear in the concentration ranges 10-50𝜇g/ml for MET, 1-5𝜇g/ml for SXG and 5-25𝜇g/ml for DGF, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76978372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00037
A. Sutar, M. B. Mangsule
Two chemometric methods PLS and PCR were used to simultaneously estimate Ofloxacin and Ornidazole in binary mixtures. Mean centering is used as a preprocessing tool and then PLS and PCR models were built with help of calibration and validation set. These methods showed good results for validation parameters including accuracy, precision, linearity, robustness etc. at the same time lower values of RMSEP, PRESS and RMSECV shows statistical significance of the developed methods. Results show that multivariate calibrations techniques can be successfully used to analyses Ofloxacin and Ornidazole in pharmaceutical formulations.
{"title":"Application of PLS and PCR as Multivariate Calibration Techniques for Simultaneous Estimation of Ofloxacin and Ornidazole in Binary Mixtures","authors":"A. Sutar, M. B. Mangsule","doi":"10.52711/2231-5675.2022.00037","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00037","url":null,"abstract":"Two chemometric methods PLS and PCR were used to simultaneously estimate Ofloxacin and Ornidazole in binary mixtures. Mean centering is used as a preprocessing tool and then PLS and PCR models were built with help of calibration and validation set. These methods showed good results for validation parameters including accuracy, precision, linearity, robustness etc. at the same time lower values of RMSEP, PRESS and RMSECV shows statistical significance of the developed methods. Results show that multivariate calibrations techniques can be successfully used to analyses Ofloxacin and Ornidazole in pharmaceutical formulations.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74503150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.52711/2231-5675.2022.00042
T. Jain, A. Vyas, D. Dubey, K. Dashora, Vishal Jain
Hab-e-Azarakhi is an important Unani formulation, is official in Unani Pharmacopoeia. The formulation is dispensed for the tone up the nervous system and liver. It is also useful for increase the appetite. Fingerprinting of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Traditional medicines/herbal formulations. Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The present study is an attempt to develop Fingerprint method for Hab-e-Azarakhi with High Performance Liquid chromatography (HPLC) using piperine as a standard was developed. The estimation was carried out with three laboratory batches and one marketed formulation of Hab-e-Azarakhi. The concentration of piperine content of Hab-e-Azarakhi (three laboratory batches) and powdered Piper nigrum and Piper longum was carried out separately. The concentration of piperine present in raw material was found to be 2.9876±0.837%w/w in Piper nigrum and 0.9363±0.167% w/w in Piper longum and in three identical laboratory batches of Hab-e- Azarakhi HA-I, HA-II and HA-III 1.8121±0.739%, 1.8806±0.439%, 1.8901±0.779% w/w respectively, while in M-I it was 1.2108 ± 0.182%. The developed HPLC method is simple, rapid, precise and accurate for routine estimation piperine in Hab-e- Azarakhi.
{"title":"Development of HPLC Fingerprinting Method via Estimation of Piperine for Unani Formulation Hab-e-Azarakhi","authors":"T. Jain, A. Vyas, D. Dubey, K. Dashora, Vishal Jain","doi":"10.52711/2231-5675.2022.00042","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00042","url":null,"abstract":"Hab-e-Azarakhi is an important Unani formulation, is official in Unani Pharmacopoeia. The formulation is dispensed for the tone up the nervous system and liver. It is also useful for increase the appetite. Fingerprinting of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Traditional medicines/herbal formulations. Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The present study is an attempt to develop Fingerprint method for Hab-e-Azarakhi with High Performance Liquid chromatography (HPLC) using piperine as a standard was developed. The estimation was carried out with three laboratory batches and one marketed formulation of Hab-e-Azarakhi. The concentration of piperine content of Hab-e-Azarakhi (three laboratory batches) and powdered Piper nigrum and Piper longum was carried out separately. The concentration of piperine present in raw material was found to be 2.9876±0.837%w/w in Piper nigrum and 0.9363±0.167% w/w in Piper longum and in three identical laboratory batches of Hab-e- Azarakhi HA-I, HA-II and HA-III 1.8121±0.739%, 1.8806±0.439%, 1.8901±0.779% w/w respectively, while in M-I it was 1.2108 ± 0.182%. The developed HPLC method is simple, rapid, precise and accurate for routine estimation piperine in Hab-e- Azarakhi.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91077308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-12DOI: 10.52711/2231-5675.2022.00034
Amit Kumar J. Vyas, Sonali B. Mishra, A. Patel, N. K. Patel, S. R. Shah, D. Sheth
Liquid Chromatography- Mass Spectrometry is a powerful analytical technique having high Sensitivity and Specificity. LC-MS combines the separation capabilities of Liquid Chromatography with the mass analysis capabilities of Mass Spectrometry. Liquid Chromatography separates the mixture, which is having multiple components, and then Mass Spectrometry provides the Spectral information which is used to identify each separated component. Interfaces are used in LC-MS system to transfer the maximum amount of analyte, remove a significant portion of the mobile phase, and preserves the chemical identity. Different types of Interfaces are used which include Atmospheric-Pressure Ionization, Electrospray Ionization, Atmospheric Pressure Chemical Ionization, Thermospray and Plasma Spray Ionization, Particle Beam Ionization, Continuous Flow Fast Atom Bombardment. LC-MS having a wide range of application in various field which include Pharmaceutical, Drug Development, Forensic Science, Cosmetic, Petrochemistry, Environment, Food Analysis, Biochemical Application. In this article Principle, Instrument and Application are discussed briefly.
{"title":"A Brief Review on Liquid Chromatography-Mass Spectrometry/LCMS and its Application","authors":"Amit Kumar J. Vyas, Sonali B. Mishra, A. Patel, N. K. Patel, S. R. Shah, D. Sheth","doi":"10.52711/2231-5675.2022.00034","DOIUrl":"https://doi.org/10.52711/2231-5675.2022.00034","url":null,"abstract":"Liquid Chromatography- Mass Spectrometry is a powerful analytical technique having high Sensitivity and Specificity. LC-MS combines the separation capabilities of Liquid Chromatography with the mass analysis capabilities of Mass Spectrometry. Liquid Chromatography separates the mixture, which is having multiple components, and then Mass Spectrometry provides the Spectral information which is used to identify each separated component. Interfaces are used in LC-MS system to transfer the maximum amount of analyte, remove a significant portion of the mobile phase, and preserves the chemical identity. Different types of Interfaces are used which include Atmospheric-Pressure Ionization, Electrospray Ionization, Atmospheric Pressure Chemical Ionization, Thermospray and Plasma Spray Ionization, Particle Beam Ionization, Continuous Flow Fast Atom Bombardment. LC-MS having a wide range of application in various field which include Pharmaceutical, Drug Development, Forensic Science, Cosmetic, Petrochemistry, Environment, Food Analysis, Biochemical Application. In this article Principle, Instrument and Application are discussed briefly.","PeriodicalId":8547,"journal":{"name":"Asian Journal of Pharmaceutical Analysis","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83953716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-12DOI: 10.52711/2231-5675.2022.00035
Darshan A. Salade, Kishor S. Arote, P. Patil, Vikas V. Patil, Amol R. Pawar
Validation is the procedure which authorizing documentary evidence that proves the following process will consistently produce the product which leads to the expected result. According to GMP validation studies are important part of GMP these are required to be done as per predefined protocols, the minimum that should be validated include process, testing and cleaning as a result such control procedure stablish to monitor the output and validation of manufacturing processes that may be responsible for variability of drug product. Validation is the one of the important part in achieving and maintaining the quality of the final product. The validation study provides the accuracy, sensitivity, specificity and reproducibility of the test methods employed by the firms, shall be established and documented. Process validation is the process for improving the safety and quality of the dosage form which is manufactured in the pharmaceutical industry. Process validation is an integral part of quality assurance as per cGMP. Validation and quality assurance will go hand in hand, ensuring the thorough quality for product. Process validation plays a key role in the pharmaceutical manufacturing process as it delivers a high degree of assurance and evidence that the process, which is being carried out gives out the uniform results, that is, it means the required specifications, which has been performed accurately. The purpose of this review is to present an introduction and general review on validation in pharmaceutical industry.
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