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Goat movement network analysis and its implications for caprine brucellosis propagation in Nonthaburi Province, Thailand 山羊运动网络分析及其对泰国芽武里省山羊布鲁氏菌病传播的影响
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-85
P. Chintrakulchai, Sirawit Vuttichai, A. Wiratsudakul
Peerawat Chintrakulchai, Sirawit Vuttichai, Anuwat Wiratsudakul Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand The Monitoring and Surveillance Centre for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand Asian Pac J Trop Dis 2017; 7(8): 477-481
玛希隆大学兽医学院临床科学与公共卫生系,泰国那空73170。玛希隆大学兽医学院野生动物和外来动物人畜共患疾病监测中心,泰国那空73170,亚洲太平洋J Trop Dis 2017;7 (8): 477 - 481
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引用次数: 3
The association of Cryptosporidium from three different points of Balok River and Kuantan River by using physico-chemical and heavy metal assessments 应用理化和重金属评价巴洛河和关丹河三个不同点隐孢子虫的亲缘关系
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-103
Fatin Khairunnisa Zainutdin, Mohd Aiman Barudin, M. A. Jainul, M. Isa, A. Yusof
Objective: The objectives of this study were to detect the occurrence of Cryptosporidium oocysts and to assess the physico-chemical and heavy metal parameters in two main rivers in Kuantan. Methods: Water samples were collected at three sampling points per river (upstream, midstream, downstream) from Kuantan River and Balok River. Samples were filtered using the Continuous Flow Centrifugation (CFC) machine followed by immunomagnetic separation technique to isolate Cryptosporidium oocysts and stained using fluorescein isothiocyanate (FITC) –staining. Cryptosporidium oocysts were examined using fluorescence microscope. Physical parameters were assessed in-situ using Cyber Scan PCD 650 multi-parameter instrument. Both chemical and heavy metal assessment were done in the laboratory following the American Public Health Association (APHA) Standard Method with slight modification. The parameters attained were compared with the Interim National Water Quality Standards (INWQS) which is the standard vital parameters used to evaluate the safety level of surface water in Malaysia. Results: All samples were positive with Cryptosporidium oocysts. Results for physical parameters were within the range of Interim National Water Quality Standards (INWQS) in Malaysia. For chemical assessment, results for chemical oxygen demand and biological oxygen demand exceeded the INWQS range in most of the sampling points. Of 23 metal elements assessed, only 9 elements were found. Both Kuantan River and Balok River can be classified under Class III river following INWQS which requires extensive treatment. Conclusions: This study hoped to provide new and updated information on the occurrence of Cryptosporidium and its physico-chemical assessment in two main rivers in Kuantan. Future study on molecular identification of Cryptosporidium in rivers needs to be done in order to identify the source of transmission of this waterborne parasite.
目的:本研究旨在检测关丹两条主要河流隐孢子虫卵囊的发生情况,并评估其理化和重金属参数。方法:在宽潭河和巴鲁克河的每条河流(上游、中游、下游)的三个采样点采集水样。使用连续流离心(CFC)机过滤样品,然后采用免疫磁分离技术分离隐孢子虫卵囊,并使用异硫氰酸荧光素(FITC)染色。用荧光显微镜检查隐孢子虫卵囊。物理参数使用Cyber Scan PCD 650多参数仪器进行现场评估。化学和重金属评估均在实验室中按照美国公共卫生协会(APHA)的标准方法进行,略有修改。将获得的参数与国家临时水质标准(INWQS)进行了比较,后者是用于评估马来西亚地表水安全水平的标准重要参数。结果:所有标本均为隐孢子虫卵囊阳性。物理参数的结果在马来西亚国家水质临时标准(INWQS)的范围内。就化学评估而言,大多数采样点的化学需氧量和生物需氧量结果都超过了INWQS范围。在评估的23种金属元素中,只发现了9种元素。关丹河和巴鲁克河都可以根据INWQS分类为三级河流,需要进行广泛的处理。结论:本研究旨在为关丹两条主要河流隐孢子虫的发生及其理化评价提供新的最新信息。未来需要对河流中隐孢子虫的分子鉴定进行研究,以确定这种水传播寄生虫的传播来源。
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引用次数: 2
A study on endoparasitic and ectoparasitic fauna of snakes in Mizoram, India 标题印度米佐拉姆邦蛇类内寄生和外寄生动物群的研究
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-48
G. Patra, S. Borthakur, S. Alam, Subhamoy Ghosh, H. Lalliankimi, H. Lalrinkima
Gautam Patra, Sonjoy Kumar Borthakur, Seikh Sahanawaz Alam, Subhamoy Ghosh, Hmar Lalliankimi, Hniang Lalrinkima Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, India Department of Veterinary Microbiology, West Bengal University of Animal & Fishery Sciences, K.B. Sarani, Kolkata, W.B., India Department of Livestock Production & Management, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, India Asian Pac J Trop Dis 2017; 7(8): 458-462
Gautam Patra, Sonjoy Kumar Borthakur, Seikh Sahanawaz Alam, Subhamoy Ghosh, Hmar Lalliankimi, hnang Lalrinkima兽医寄生虫学系,印度兽医科学与畜牧学院,印度兽医微生物学系,西孟加拉邦动物与渔业科学大学,K.B. Sarani,加尔各答,印度畜牧生产与管理系,印度兽医科学与畜牧学院,Selesih, Aizawl2017;印度;亚太地区;7 (8): 458 - 462
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引用次数: 3
Liposome as nanocarrier: Site targeted delivery in lung cancer 脂质体作为纳米载体:肺癌部位靶向递送
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D6-182
N. Ullah, Saba Noreen, K. Tehreem, A. Zaman, Z. Ahmad, H. Balouch, N. Samad
The phenomenal perceptive and study of how to handle the materials at molecular, macromolecular and atomic levels, possessing characteristics which are appreciably discriminated from the higher levels, is notorious as nanoscience. The applications of these technologies for cancer handling are drug delivery modifications and overpowering some drug delivery disorders[1]. One billionth of a meter (a nanometer) and this subatomic and atomic level, has the material features which are evidently distinctive from features of larger sized forms of the same materials. The innovative traits haunted by nanomaterials, though, renowned were for its mechanical, electrical, physical, magnetic, biological and chemical applications, presently, pharmaceutical purposes, mainly in drug delivery field, are getting a much more dedication[2]. Extensive research has been done for many eras in cancer mitigation using nanotechnology that is moving ahead quickly up to now. The pharmacokinetics individuality of many chemotherapeutic medications that are potentially functioning with less aqueous solubility, can be enhanced by nanocarriers predominantly. In this way the therapeutic effect of these products is being boosted. Hence, a multiplicity of drug delivery systems based on the biocompatible polymeric resources has been anticipated[3]. The most substantial research field for the pharmaceutical drugs management and development is targeted drug delivery system (TDDS). TDDS has the major role in drug delivery to the targeted tissues while comparative medication strength is reduced in other remaining tissues subsequent this. Consequently, leaving the neighboring tissues uninfluenced, the drug is mainly confined to the targeted area. The TDDS advantages, mainly include safe and welfare consignment and perfection in therapeutic catalog. A greater variety of quantitative advantages of TDDS for disease therapy are also explored[4]. The four most frequently identified cancers are prostate, breast, colorectal and lung cancers. Plentiful hostile effects of chemotherapeutic drugs are also pragmatic. Nanotechnology has a great exploitation in medical sciences as it is the escalating filed in medicine[5]. Cancer is one of the miscellaneous and heterogeneous diseases. Drug withstanding is the consequence of the synchronized practice of multiple drugs which is known as multidrug resistance (MDR) and it can be either intrinsic or acquired. Cancerous cells are being immune to typical therapy due to MDR with many anticancer mechanisms. Now it is the foremost routine in cancer medication to ARTICLE INFO ABSTRACT
对如何在分子、大分子和原子水平上处理材料的非凡感知和研究,具有明显区别于更高水平的特征,被称为纳米科学。这些技术在癌症治疗中的应用是药物递送修饰和克服某些药物递送障碍[1]。十亿分之一米(纳米)和这种亚原子和原子水平的材料特征与相同材料的较大尺寸形式的特征明显不同。然而,纳米材料的创新特性以其机械、电气、物理、磁性、生物和化学应用而闻名,目前,制药目的,主要是在药物递送领域,正变得更加专注[2]。使用纳米技术缓解癌症已经进行了许多时代的广泛研究,目前进展迅速。许多化疗药物的药代动力学特性可能具有较小的水溶性,主要可以通过纳米载体来增强。通过这种方式,这些产品的治疗效果得到了提高。因此,基于生物相容性聚合物资源的多种药物递送系统已被预期[3]。药物管理和开发最重要的研究领域是靶向给药系统(TDDS)。TDDS在药物递送到靶组织中具有主要作用,而随后在其他剩余组织中的相对药物强度降低。因此,在不影响邻近组织的情况下,药物主要局限于靶区。TDDS的优势,主要包括安全、福利的托运和完善的治疗目录。TDDS在疾病治疗中的各种量化优势也得到了探索[4]。四种最常见的癌症是前列腺癌、乳腺癌、结直肠癌和肺癌。化疗药物的大量敌对作用也是实用的。纳米技术是医学领域不断发展的一个领域,在医学科学中有着巨大的开发潜力[5]。癌症是多种疾病中的一种。药物耐受是多种药物同步使用的结果,这种药物被称为多药耐药性(MDR),它可以是内在的,也可以是后天的。由于MDR具有许多抗癌机制,癌细胞对典型的治疗具有免疫性。现在这是癌症治疗的首要常规
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引用次数: 3
Phenotypic and molecular detection of multi-drug resistant Salmonella Enteritidis, Salmonella Typhimurium and Salmonella species in retail raw beef and chicken 零售生牛肉和鸡肉中多重耐药肠炎沙门氏菌、鼠伤寒沙门氏菌和沙门氏菌的表型和分子检测
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-10
R. E. Uzeh, Venatius Chinenye Ihekire, S. Smith, M. Fowora
Globally, Salmonella is a major cause of foodborne diseases[1,2]. The incidence of non-typhoidal Salmonella is estimated at 1.3 billion cases with annual death rate of 3 million[3]. It results in morbidity, mortality and great economic loss[4,5]. Human salmonellosis is most frequently caused by Salmonella Typhimurium (S. Typhimurium) and Salmonella Enteritidis (S. Enteritidis)[6]. Among the over 2 500 serovars identified within Salmonella enterica subspecies enterica, S. Typhimurium continues to be one of the most frequently recovered from food animals worldwide[7]. Due to its broad host range, S. Typhimurium is also one of the most common serotype isolated from human clinical cases of food-borne salmonellosis. Poor sanitary conditions have been identified to be responsible for the transmission of Salmonella spp., S. Typhimurium (group D) and S. Enteritidis (group B) in developing countries. In sub-Saharan Africa, they have been reported to be the cause of 79%–95% of all bacteriaemic non-typhoidal Salmonella infections or foodborne outbreaks[8,9], and are associated with case fatality rate of 20%– 25%[10]. Salmonella can be transmitted to humans from animals and by consuming foods from animal sources such as milk, egg, poultry meat and beef which serve as reservoirs[11,12]. During the production of meat, the major source of Salmonella contamination of carcasses is the evisceration stage in slaughter house[13]. In order to ensure food safety and for the purpose of food borne disease surveillance, foods should be examined routinely for the presence of Salmonella. Conventional typing methods such as, biotyping, serotyping and phage typing which are based on phenotypic characteristics have been used extensively for this purpose[14]. However, they are less discriminative. Molecular typing methods offer higher discrimination[14] and have been employed for identification of Salmonella spp.[9]. Studies on the molecular typing of microbial isolates have ARTICLE INFO ABSTRACT
在全球范围内,沙门氏菌是食源性疾病的主要原因[1,2]。非伤寒沙门氏菌的发病率估计为13亿例,年死亡率为300万[3]。它会导致发病率、死亡率和巨大的经济损失[4,5]。人类沙门氏菌病最常见由鼠伤寒沙门氏菌(S.Typhimurium)和肠炎沙门氏菌引起[6]。在肠炎沙门氏菌亚种肠炎中发现的2500多种血清型中,鼠伤寒沙门氏菌仍然是世界上最常见的食用动物之一[7]。由于其宿主范围广泛,鼠伤寒沙门氏菌也是从人类食源性沙门氏菌病临床病例中分离出的最常见的血清型之一。卫生条件差已被确定是沙门氏菌属、鼠伤寒沙门氏菌(D组)和肠炎沙门氏杆菌(B组)在发展中国家传播的原因。据报道,在撒哈拉以南非洲,它们是所有细菌性非伤寒沙门氏菌感染或食源性疫情的79%-95%[8,9]的病因,病死率为20%-25%[10]。沙门氏菌可以通过动物和食用动物来源的食物传播给人类,如牛奶、鸡蛋、禽肉和牛肉,这些食物是宿主[11,12]。在肉类生产过程中,胴体沙门氏菌污染的主要来源是屠宰场的内脏摘除阶段[13]。为了确保食品安全和监测食源性疾病,应定期检查食品中是否存在沙门氏菌。基于表型特征的传统分型方法,如生物分型、血清分型和噬菌体分型,已被广泛用于此目的[14]。然而,他们的歧视性较小。分子分型方法提供了更高的辨别力[14],并已用于鉴定沙门氏菌。[9]。对微生物分离株分子分型的研究有文章信息摘要
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引用次数: 1
Soluble expression and purification of hepatitis B core antigen (HBcAg) subgenotype B3 in Escherichia coli using thioredoxin fusion tag 硫氧还蛋白融合标签在大肠杆菌中可溶性表达和纯化乙型肝炎核心抗原B3亚基因
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-58
Rahmah Waty, A. Z. Mustopa, S. Suharsono, Hidayah Murtiyaningsih
Hepatitis B virus (HBV) infection is a serious health problem in the world. HBV is one of the major causes of liver diseases because of its long term effects. The HBV infection in liver can cause acute or chronic disease. It can be transmitted through contact with blood or other body fluids of hepatitis patient. It was estimated that 257 million people worldwide have been infected with HBV. In 2015, 887 000 deaths were caused by hepatitis and its complications such as cirrhosis and hepatocellular carcinoma[1]. Besides, the prevalence of HBV infections in Southeast Asia including Indonesia is high[2]. Genetic variability of HBV plays an important role in the development of HBV infection treatment. Based on nucleotide array, HBV is classified into eight genotypes (A–H) and each genotype is classified into some subgenotypes. Each genotype or subgenotype has a different geographic distribution. For example, some HBV/ B subgenotypes from Southeast Asia (B3, B4, B5, B7, B8, and B9) are clearly separated with HBV/B1 and B2 subgenotypes which are found in Japan and China[3]. Indonesia is an archipelago that consists of thousands of islands and is a home to hundreds of ethnic populations. The distribution of HBV genotypes/subgenotypes in the Indonesian archipelago is related to the ethnic pattern of its population. There are three main genotypes of HBV in Indonesia, namely, HBV/B (70.9%), HBV/C (27.5%), and HBV/D (1.6%). HBV/B is the dominant genotype in Western Indonesia, especially HBV/B3 which is the predominant subgenotype in Java region. In East Indonesia, HBV/C is the predominant genotype followed by HBV/D[3,4]. ARTICLE INFO ABSTRACT
乙型肝炎病毒(HBV)感染是世界范围内严重的健康问题。HBV因其长期影响而成为肝脏疾病的主要原因之一。乙肝病毒感染肝脏可引起急性或慢性疾病。它可以通过接触肝炎患者的血液或其他体液传播。据估计,全世界有2.57亿人感染了乙肝病毒。2015年,有88.7万人死于肝炎及其并发症,如肝硬化和肝细胞癌。此外,包括印度尼西亚在内的东南亚地区乙型肝炎病毒感染率很高。HBV的遗传变异在HBV感染治疗的发展中起着重要作用。基于核苷酸阵列,HBV分为8个基因型(A-H),每个基因型又分为一些亚基因型。每种基因型或亚基因型都有不同的地理分布。例如,来自东南亚的一些HBV/B亚基因型(B3、B4、B5、B7、B8和B9)与在日本和中国发现的HBV/B1和B2亚基因型明显分离。印度尼西亚是一个由数千个岛屿组成的群岛,是数百个少数民族的家园。HBV基因型/亚基因型在印度尼西亚群岛的分布与其人口的种族模式有关。印度尼西亚的HBV主要有三种基因型,即HBV/B(70.9%)、HBV/C(27.5%)和HBV/D(1.6%)。HBV/B是印度尼西亚西部的主要基因型,特别是HBV/B3是爪哇地区的主要亚基因型。在东印度尼西亚,HBV/C是主要的基因型,其次是HBV/D[3,4]。文章信息摘要
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引用次数: 2
Characterization of diarrheagenic E. coli causing a diarrheal outbreak in the south of Iran, Summer 2015 2015年夏季伊朗南部引起腹泻疫情的致泻性大肠杆菌的特征
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-94
G. Pouladfar, Amir Arasteh-Far, M. Aminshahidi, N. Firoozian, B. Pourabbas, M. Moghadami, F. Fani
Gholamreza Pouladfar, Amir Arasteh-Far, Maneli Amin-Shahidi, Nasrin Firoozian, Bahman Pourabbas, Mohsen Moghadami, Fereshteh Fani Professor Alborzi Clinical Microbiology Research Center, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Fars, Iran Health Policy Research Center, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran Asian Pac J Trop Dis 2017; 7(8): 491-495
Gholamreza Pouladfar, Amir Arasteh-Far, Maneli Amin-Shahidi, Nasrin Firoozian, Bahman Pourabbas, Mohsen Moghadami, Fereshteh Fani教授设拉子医科大学医学院Alborzi临床微生物学研究中心,设拉子,法尔斯,伊朗设拉子医科大学卫生政策研究中心,设拉子,伊朗伊斯兰共和国7 (8): 491 - 495
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引用次数: 2
Are species-specific antigen detection tests needed in the diagnosis of Giardia duodenalis infection? 诊断十二指肠贾第鞭毛虫感染是否需要进行物种特异性抗原检测?
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-102
F. Uchôa, A. Sudré, N. Almosny
Flávia Fernandes de Mendonça Uchôa, Adriana Pittella Sudré, Nádia Regina Pereira Almosny Postgraduate Program in Veterinary Clinics and Animal Reproduction, School of Veterinary Medicine, Fluminense Federal University (UFF), Niterói, RJ, Brasil Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University (UFF), Niterói, RJ, Brasil Department of Clinics and Surgery, School of Veterinary Medicine, Fluminense Federal University (UFF), Niterói, RJ, Brasil Asian Pac J Trop Dis 2017; 7(8): 455-457
Flávia Fernandes de Mendonça Uchôa,Adriana Pittella Sudré,Nádia Regina Pereira Almosny兽医诊所和动物繁殖研究生项目,弗鲁米嫩塞联邦大学兽医学院,尼日利亚,巴西微生物和寄生虫学系,生物医学研究所,弗鲁米嫩塞联邦大学兽医学院巴西临床与外科,2017年巴西亚洲太平洋医学会;7(8):455-457
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引用次数: 1
Prevalence of intestinal parasites among food handlers at cafeteria of Jimma University Specialized Hospital, Southwest Ethiopia 埃塞俄比亚西南部吉马大学专科医院食堂食品处理人员肠道寄生虫流行情况
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-20
Hundaol Girma, Getenet Beyene, Z. Mekonnen
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引用次数: 18
Antimicrobial susceptibility patterns and CTX-M β-lactamase producing clinical isolates from burn patients in Islamabad, Pakistan 巴基斯坦伊斯兰堡烧伤患者的耐药性模式和产CTX-Mβ-内酰胺酶的临床分离株
Pub Date : 2017-08-10 DOI: 10.12980/APJTD.7.2017D7-75
M. Hussain, S. Munir, M. Fatima, Kashif Rahim, I. Ahmed, A. Basit, M. Z. Talpur, Nawaz Haider Bashir, Arslan Jamil, M. Jamal
Mubbashir Hussain, Shahzad Munir, Madiha Fatima, Kashif Rahim, Irfan Ahmed, Abdul Basit, Mir Zulqarnain Talpur, Nawaz Haider Bashir, Arslan Jamil, Muhammad Ameen Jamal Department of Microbiology, Kohat University of Science and Technology Kohat, KP, 26000, Pakistan Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan, China State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150069, China
Mubbashir Hussain,Shahzad Munir,Madiha Fatima,Kashif Rahim,Irfan Ahmed,Abdul Basit,Mir Zulqarnain Talpur,Nawaz Haider Bashir,Arslan Jamil,Muhammad Ameen Jamal Kohat科技大学微生物系,KP,26000,云南农业大学巴基斯坦植物保护学院,云南昆明650201,中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨哈平路678号,邮编150069
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引用次数: 5
期刊
Asian Pacific Journal of Tropical Disease
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