Pub Date : 2020-12-01DOI: 10.21608/aunj.2020.220877
M. Bysam, A. Sedky, W. Elshemey, H. Mohammed
The low-level laser therapy was used in various biomedical applications for a long time such as improving the rheological properties of blood, wound healing, vascular restenosis, tissue repair. however, its safety has not been well investigated. The present in-vitro study aims to illustrate the influence of low power laser irradiation on the secondary structure of human Haemoglobin (Hb). Fifteen samples of healthy fresh blood have been divided into five groups (3 samples/group) and irradiated using low power infrared laser (wavelength 808 nm, power 4 mw and power density 0.64 mW/cm 2 ) at different irradiation durations (5, 10, 20, 30 min) and zero time for control samples. We used FTIR Spectroscopy followed by some mathematical techniques such as band-narrowing (second-derivative method), spectral subtraction, curve fitting, and integration processes to quantitatively calculate the different contribution ratios of the secondary structure components of irradiated and non-irradiated human hemoglobin samples. It was found no significant change in the contents of the α-helix structure at (5,10,20 min) groups. However, the thirty minutes group (30min) show a significant reduction in the content of α -helix accompanied by a significant increase in the random coil and β-turn structures with (p < 0.05). The decreasing of the alpha-helix structure is explained in terms of photo-inductive degradation to the unordered structure of random coils and β-turns. Extra care has to be taken while the clinical application that includes the intravenous laser irradiation of blood to avoid the protein photodamage caused by the low power laser at long expoture time.
{"title":"FTIR SPECTROSCOPY STUDY ON THE IMPACT OF LOW POWER NEAR-INFRARED LASER ON THE SECONDARY STRUCTURE OF HUMAN HEMOGLOBIN (IN-VITRO)","authors":"M. Bysam, A. Sedky, W. Elshemey, H. Mohammed","doi":"10.21608/aunj.2020.220877","DOIUrl":"https://doi.org/10.21608/aunj.2020.220877","url":null,"abstract":"The low-level laser therapy was used in various biomedical applications for a long time such as improving the rheological properties of blood, wound healing, vascular restenosis, tissue repair. however, its safety has not been well investigated. The present in-vitro study aims to illustrate the influence of low power laser irradiation on the secondary structure of human Haemoglobin (Hb). Fifteen samples of healthy fresh blood have been divided into five groups (3 samples/group) and irradiated using low power infrared laser (wavelength 808 nm, power 4 mw and power density 0.64 mW/cm 2 ) at different irradiation durations (5, 10, 20, 30 min) and zero time for control samples. We used FTIR Spectroscopy followed by some mathematical techniques such as band-narrowing (second-derivative method), spectral subtraction, curve fitting, and integration processes to quantitatively calculate the different contribution ratios of the secondary structure components of irradiated and non-irradiated human hemoglobin samples. It was found no significant change in the contents of the α-helix structure at (5,10,20 min) groups. However, the thirty minutes group (30min) show a significant reduction in the content of α -helix accompanied by a significant increase in the random coil and β-turn structures with (p < 0.05). The decreasing of the alpha-helix structure is explained in terms of photo-inductive degradation to the unordered structure of random coils and β-turns. Extra care has to be taken while the clinical application that includes the intravenous laser irradiation of blood to avoid the protein photodamage caused by the low power laser at long expoture time.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76493377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220859
G. Salaheldin, Hany El-Gamal, E. Zahran, Marwa Abdel, Hamid
The objective of the present study is to measure the radioactivity concentrations of the natural radionuclides 226 Ra, 232 Th and 40 K in the Gabal Ghareb region in the eastern desert of Egypt. The activity concentrations were measured by using a high purity germanium (HPGe) detector-based γ-spectrometry. The activity concentrations of 226 Ra, 232 Th and 40 K for the collected rock samples were varied from 6.3±0.32 to 136.73 ± 6.80 Bqkg -1 with an average value of 45.75±2.28 Bqkg -1 , from 6.05±0.35 to 120.10±6.01 Bqkg -1 with an average value of 50.91±2.56 Bqkg -1 , and from 377.54±18.87 to 1102.01±55.10 Bqkg -1 with an average value of 826.13±39.33 Bqkg -1 , respectively. The radiological risk parameters such as radium equivalent (Raeq), external risk index (Hex), internal risk index (Hin), absorbed dose rate (ADR), annual effective dose rate (AEDR), annual gonadal dose equivalent (AGDE) and Excess Lifetime Cancer Risk (ELCR) were calculated to assess the radiation risks associated with the studied rock samples. Most of these parameters are higher than the recommended reference levels. The data obtained provide a valuable future database to estimate the impact of radioactive contamination in the studied area and in the places where the rocks are used.
{"title":"ASSESSMENT OF RADIATION HAZARDS OF RADIONUCLIDES FOR GRANITE ROCKS FROM GABAL GHAREB, EASTERN DESERT OF EGYPT","authors":"G. Salaheldin, Hany El-Gamal, E. Zahran, Marwa Abdel, Hamid","doi":"10.21608/aunj.2020.220859","DOIUrl":"https://doi.org/10.21608/aunj.2020.220859","url":null,"abstract":"The objective of the present study is to measure the radioactivity concentrations of the natural radionuclides 226 Ra, 232 Th and 40 K in the Gabal Ghareb region in the eastern desert of Egypt. The activity concentrations were measured by using a high purity germanium (HPGe) detector-based γ-spectrometry. The activity concentrations of 226 Ra, 232 Th and 40 K for the collected rock samples were varied from 6.3±0.32 to 136.73 ± 6.80 Bqkg -1 with an average value of 45.75±2.28 Bqkg -1 , from 6.05±0.35 to 120.10±6.01 Bqkg -1 with an average value of 50.91±2.56 Bqkg -1 , and from 377.54±18.87 to 1102.01±55.10 Bqkg -1 with an average value of 826.13±39.33 Bqkg -1 , respectively. The radiological risk parameters such as radium equivalent (Raeq), external risk index (Hex), internal risk index (Hin), absorbed dose rate (ADR), annual effective dose rate (AEDR), annual gonadal dose equivalent (AGDE) and Excess Lifetime Cancer Risk (ELCR) were calculated to assess the radiation risks associated with the studied rock samples. Most of these parameters are higher than the recommended reference levels. The data obtained provide a valuable future database to estimate the impact of radioactive contamination in the studied area and in the places where the rocks are used.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84730113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220871
H. Omar, Asmaa M Mohamed, Heba M Saadeldien, E. Wassif, O. Omran, E. Ahmed
Potassium bromate (KBrO3) is used in many countries in cosmetic and food industries. In this research, we study the possible renoprotective effect of curcumin (CUR) and bone marrow meschenymal stem cells (BM-MSCs) on the actions of KBrO3 in female rats. Thirty two female rats were categorized into four group, the first work as control, the second was exposed to KBrO3 (100 mg/kg/day for 28 days in drinking water), the third and fourth where exposed to KBrO3 like group 2 and co-treated with either CUR (100mg/kg, ip) twice a week or BM-MSCs (2x10 6 , ip for each rat) once a week for 4 weeks, respectively. Kidney function and oxidative stress parameters were measured calorimetrically in plasma. Expression of caspase-3 in kidney by real time PCR was measured by the comparative Ct (2-ΔΔCt) method. Apoptosis in kidney was evaluated by TUNNEL assay. The results indicated that treatment with KBrO3 caused nephrotoxicity, as evident by the measured renal structural and functional indices and oxidative stress markers in plasma. CUR and BM-MSCs co-treatment significantly abated most of the indices and biomarkers of the renal toxicity caused by KBrO3, suggesting their beneficial effects with the priority of CUR due to their antioxidant effect.
{"title":"PROTECTIVE EFFICIENCY OF CURCUMIN AND BONE MARROW MESENCHYMAL STEM CELLS OF NEPHROTOXICITY INDUCED BY BROMATE IN RATS","authors":"H. Omar, Asmaa M Mohamed, Heba M Saadeldien, E. Wassif, O. Omran, E. Ahmed","doi":"10.21608/aunj.2020.220871","DOIUrl":"https://doi.org/10.21608/aunj.2020.220871","url":null,"abstract":"Potassium bromate (KBrO3) is used in many countries in cosmetic and food industries. In this research, we study the possible renoprotective effect of curcumin (CUR) and bone marrow meschenymal stem cells (BM-MSCs) on the actions of KBrO3 in female rats. Thirty two female rats were categorized into four group, the first work as control, the second was exposed to KBrO3 (100 mg/kg/day for 28 days in drinking water), the third and fourth where exposed to KBrO3 like group 2 and co-treated with either CUR (100mg/kg, ip) twice a week or BM-MSCs (2x10 6 , ip for each rat) once a week for 4 weeks, respectively. Kidney function and oxidative stress parameters were measured calorimetrically in plasma. Expression of caspase-3 in kidney by real time PCR was measured by the comparative Ct (2-ΔΔCt) method. Apoptosis in kidney was evaluated by TUNNEL assay. The results indicated that treatment with KBrO3 caused nephrotoxicity, as evident by the measured renal structural and functional indices and oxidative stress markers in plasma. CUR and BM-MSCs co-treatment significantly abated most of the indices and biomarkers of the renal toxicity caused by KBrO3, suggesting their beneficial effects with the priority of CUR due to their antioxidant effect.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78482816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220867
Naeima M. H. Yousef, E. S. Aldaby, Eman E. Ali
The present work was carried out to isolate the cellulose-decomposing bacteria (CDB) from Wadi El-Natrun, Egypt. Totally 40 isolates were isolated from saline mud samples. Eight out of 40 isolates exhibited high hydrolysis capacity of the Congo-Red screening method. The highly producer isolate (CDB2) was selected to optimize some nutritional and environmental factors for improving cellulase production. The bacterial isolate was identified on the basis of molecular characteristics using 16srRNA. The sequence analysis showed 99% similarity to Brevibacterium halotolerans, which is then deposited in the GenBank in NCBI under the accession number MN148622. The bacterium showed highly cellulase production by using carboxymethyl cellulose (CMC) as a sole carbon source. The results showed that the optimum conditions for cellulase production by the selected isolate were noticed when grown on CMC (20 g/L) at pH 8.0 incubated at 35 ο C in batch conditions with shaking. The results revealed that, the bacterial isolate showed potential cellulase production at stationary phase of growth corresponding to an incubation period of 3-5 days. The cellulase enzyme has high specificity to CMC considered to be an endo-1,4-glucanase.
本研究从埃及Wadi El-Natrun分离纤维素分解细菌(CDB)。从盐泥样品中分离到40株。40个分离株中有8个在刚果红筛选方法中表现出较高的水解能力。选择高产分离菌(CDB2)进行营养和环境因素优化，以提高纤维素酶的产量。利用16srRNA进行分子特征鉴定。序列分析结果显示，该菌株与耐盐短杆菌相似度达99%，并将其保存在NCBI的GenBank中，登录号为MN148622。该细菌以羧甲基纤维素(CMC)为唯一碳源，高产纤维素酶。结果表明，所选菌株在35 ο C、pH 8.0、CMC (20 g/L)培养基上分批培养、振荡培养，获得了最佳的产酶条件。结果表明，该分离菌在3 ~ 5 d的固定生长阶段具有生产纤维素酶的潜力。纤维素酶对CMC有很高的特异性，被认为是内切-1,4-葡聚糖酶。
{"title":"OPTIMIZATION OF THE CELLULASE ENZYME PRODUCTION BY BREVIBACTERIUM HALOTOLERANS ISOLATED FROM WADI EL-NATRUN, EGYPT","authors":"Naeima M. H. Yousef, E. S. Aldaby, Eman E. Ali","doi":"10.21608/aunj.2020.220867","DOIUrl":"https://doi.org/10.21608/aunj.2020.220867","url":null,"abstract":"The present work was carried out to isolate the cellulose-decomposing bacteria (CDB) from Wadi El-Natrun, Egypt. Totally 40 isolates were isolated from saline mud samples. Eight out of 40 isolates exhibited high hydrolysis capacity of the Congo-Red screening method. The highly producer isolate (CDB2) was selected to optimize some nutritional and environmental factors for improving cellulase production. The bacterial isolate was identified on the basis of molecular characteristics using 16srRNA. The sequence analysis showed 99% similarity to Brevibacterium halotolerans, which is then deposited in the GenBank in NCBI under the accession number MN148622. The bacterium showed highly cellulase production by using carboxymethyl cellulose (CMC) as a sole carbon source. The results showed that the optimum conditions for cellulase production by the selected isolate were noticed when grown on CMC (20 g/L) at pH 8.0 incubated at 35 ο C in batch conditions with shaking. The results revealed that, the bacterial isolate showed potential cellulase production at stationary phase of growth corresponding to an incubation period of 3-5 days. The cellulase enzyme has high specificity to CMC considered to be an endo-1,4-glucanase.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81876802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220861
Aly A. Abd-Ella, Saoud, A. Metwally, Y. A. El-Ossaily, Fathy M. Abd Elrazek, S. Aref, Youssra A. Naffea
Four 3,5-pyrazolidinedione derivatives namely, 4-(4`-chlorobenzylidene)-1-phenylpyrazolidine3,5-dione (1), 4-(4`-nitrobenzylidene)-1-phenylpyrazolidine-3,5-dione (2), 2’-(4-chlorophenyl)-1phenyl-3,5-dioxo-1,2,3,5 tetrahydrospiropyrazole-4,3’ oxirane (3) and 2’-(4-nitrophenyl)-1phenyl-3,5-dioxo-1,2,3,5 tetrahydrospiropyrazole-4,3’ oxirane (4) were prepared in pure state and bioassayed against 2 nd and 4 th instars larvae of cotton leaf worm, Spodoptera littoralis (Boised) (Lepidoptera: Noctuidae) using feeding and dipping bioassay. The results of bioassays indicated that title compounds exhibit satisfactory insecticidal activities. Among those, compound (1) exhibit the highest insecticidal activities against 2 nd instar larvae, with LC50s 3.23 and 0.619 mgL -1 for feeding, and 36.04 and 28.69 mgL -1 for dipping, after 48 and 72 h treatment. According to the toxicity index the compound (1) showed the highest larvicidal activity against 4 th instar larvae with LC50s 141.33 and 76.12 mgL -1 for feeding larvae, and 26.94 and 12.29 mgL -1 for dipping larvae after 48 and 72 h treatment. These results showed that, the 2 nd larvae was more susceptible than 4 th instars larvae to these compounds. In addition, the insecticidal activity of these compounds was Aly A. Abd-Ella et al. 2 more effective on cotton leaf worm larvae in feeding bioassay as compared with the dipping treatment. The rest of the tested compounds possessed moderate to strong larvicidal activities against cotton leaf worm. In general, the results indicate the possible use of 3,5-pyrazolidinedione derivatives as components in integrated pest management program against S. littoralis.
{"title":"Insecticidal activity of some 3, 5-pyrazolidinedione derivatives against cotton leaf worm, Spodoptera littoralis (Boised) (Lepidoptera: Noctuidae)","authors":"Aly A. Abd-Ella, Saoud, A. Metwally, Y. A. El-Ossaily, Fathy M. Abd Elrazek, S. Aref, Youssra A. Naffea","doi":"10.21608/aunj.2020.220861","DOIUrl":"https://doi.org/10.21608/aunj.2020.220861","url":null,"abstract":"Four 3,5-pyrazolidinedione derivatives namely, 4-(4`-chlorobenzylidene)-1-phenylpyrazolidine3,5-dione (1), 4-(4`-nitrobenzylidene)-1-phenylpyrazolidine-3,5-dione (2), 2’-(4-chlorophenyl)-1phenyl-3,5-dioxo-1,2,3,5 tetrahydrospiropyrazole-4,3’ oxirane (3) and 2’-(4-nitrophenyl)-1phenyl-3,5-dioxo-1,2,3,5 tetrahydrospiropyrazole-4,3’ oxirane (4) were prepared in pure state and bioassayed against 2 nd and 4 th instars larvae of cotton leaf worm, Spodoptera littoralis (Boised) (Lepidoptera: Noctuidae) using feeding and dipping bioassay. The results of bioassays indicated that title compounds exhibit satisfactory insecticidal activities. Among those, compound (1) exhibit the highest insecticidal activities against 2 nd instar larvae, with LC50s 3.23 and 0.619 mgL -1 for feeding, and 36.04 and 28.69 mgL -1 for dipping, after 48 and 72 h treatment. According to the toxicity index the compound (1) showed the highest larvicidal activity against 4 th instar larvae with LC50s 141.33 and 76.12 mgL -1 for feeding larvae, and 26.94 and 12.29 mgL -1 for dipping larvae after 48 and 72 h treatment. These results showed that, the 2 nd larvae was more susceptible than 4 th instars larvae to these compounds. In addition, the insecticidal activity of these compounds was Aly A. Abd-Ella et al. 2 more effective on cotton leaf worm larvae in feeding bioassay as compared with the dipping treatment. The rest of the tested compounds possessed moderate to strong larvicidal activities against cotton leaf worm. In general, the results indicate the possible use of 3,5-pyrazolidinedione derivatives as components in integrated pest management program against S. littoralis.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76155953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220869
M. Ismail, Nagwa R. A. Hussein, M. Abdel‐Sater, RM Sayed
Mycobiota of 25 soil samples, collected from Botanical garden of Botany and Microbiology Department, Faculty of Science, Assiut University, were monitored on acidified weak potato dextrose agar (AWPDA), dichloran chloramphenicol peptone agar (DCPA), dichloran glycerol agar (DG-18) and potato carrot agar with manganese (PCA-Mn) media at 25 oC. One hundred and twenty-two species belong to 47 genera were obtained. Aspergillus was the most predominant genus on all media and A. niger was superior on AWPDA, DG18 and PCA-Mn. Fusarium was the runner up, followed by Alternaria, Curvularia, Talaromyces, Penicillium and Stachybotrys. Fifteen isolates of six species of Alternaria were subjected to some physiological and biochemical tests. The statistically analyzed results showed that, all isolates could significantly grow on ammonium tartarate, ammonium oxalate and glycine, while 14, 13 and 11 isolates wer able to grow on citric acid, lactic acid and sodium nitrite, respectively. Alternaria tenuissima and A. angustiovoidea were highly base producers from ammonium tartarate, while, the maximum base production on glycine was observed by A. angustiovoidea and A. arborescens. All isolates enabled to grow on creatine-sucrose and A. arborescens produced the strongest color, whereas the remaining isolates were negative (11 isolates) to moderate (3). On the other hand, A. chlamydospora and A. angustiovoidea showed maximum growth on mannitol and tannin-sucrose, respectively. Besides, the growth on 15 % NaCl-Cz was distinctive for 60 % and 50 % of A. alternata and A. angustiovoidea isolates, respectively.
{"title":"EVALUATION OF PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF ALTERNARIA SPECIES ISOLATED FROM SOIL IN ASSIUT GOVERNORATE, EGYPT, IN ADDITION TO DICHOTOMOUS KEY TO THE ENCOUNTERED SPECIES","authors":"M. Ismail, Nagwa R. A. Hussein, M. Abdel‐Sater, RM Sayed","doi":"10.21608/aunj.2020.220869","DOIUrl":"https://doi.org/10.21608/aunj.2020.220869","url":null,"abstract":"Mycobiota of 25 soil samples, collected from Botanical garden of Botany and Microbiology Department, Faculty of Science, Assiut University, were monitored on acidified weak potato dextrose agar (AWPDA), dichloran chloramphenicol peptone agar (DCPA), dichloran glycerol agar (DG-18) and potato carrot agar with manganese (PCA-Mn) media at 25 oC. One hundred and twenty-two species belong to 47 genera were obtained. Aspergillus was the most predominant genus on all media and A. niger was superior on AWPDA, DG18 and PCA-Mn. Fusarium was the runner up, followed by Alternaria, Curvularia, Talaromyces, Penicillium and Stachybotrys. Fifteen isolates of six species of Alternaria were subjected to some physiological and biochemical tests. The statistically analyzed results showed that, all isolates could significantly grow on ammonium tartarate, ammonium oxalate and glycine, while 14, 13 and 11 isolates wer able to grow on citric acid, lactic acid and sodium nitrite, respectively. Alternaria tenuissima and A. angustiovoidea were highly base producers from ammonium tartarate, while, the maximum base production on glycine was observed by A. angustiovoidea and A. arborescens. All isolates enabled to grow on creatine-sucrose and A. arborescens produced the strongest color, whereas the remaining isolates were negative (11 isolates) to moderate (3). On the other hand, A. chlamydospora and A. angustiovoidea showed maximum growth on mannitol and tannin-sucrose, respectively. Besides, the growth on 15 % NaCl-Cz was distinctive for 60 % and 50 % of A. alternata and A. angustiovoidea isolates, respectively.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80685904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220855
Eman Baky, Emad Mabrouk, I. Elsemman
Classification is one of the most popular techniques of data mining. This paper presents an evolutionary approach for designing classifiers for two-class classification problems using an enhanced version of the genetic programming (GP) algorithm, called the Memetic Programming (MP) algorithm. MP can discover relationships between observed data and express them logically. MP aims to obtain a classifier with the largest area under the ROC curve, which has been proved a better performance than traditionally metrics. The proposed approach is being demonstrated by experimenting on some UCI Machine Learning data sets. Results obtained in these experiments reflect the efficiency of the proposed algorithm.
{"title":"MEMETIC PROGRAMMING WITH THE ATOMIC REPRESENTATION FOR EXTRACTING LOGICAL CLASSIFICATION RULES","authors":"Eman Baky, Emad Mabrouk, I. Elsemman","doi":"10.21608/aunj.2020.220855","DOIUrl":"https://doi.org/10.21608/aunj.2020.220855","url":null,"abstract":"Classification is one of the most popular techniques of data mining. This paper presents an evolutionary approach for designing classifiers for two-class classification problems using an enhanced version of the genetic programming (GP) algorithm, called the Memetic Programming (MP) algorithm. MP can discover relationships between observed data and express them logically. MP aims to obtain a classifier with the largest area under the ROC curve, which has been proved a better performance than traditionally metrics. The proposed approach is being demonstrated by experimenting on some UCI Machine Learning data sets. Results obtained in these experiments reflect the efficiency of the proposed algorithm.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73116467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220865
N. Youssef, A. Ismail, Naglaa Adly Hussien
It was found for decades that phytohormones have a prime role in modifying the plant growth. Especially, when these plants are exposed to biotic or abiotic stress. Verify this fact, this study examined the effect of salinity (0.0, 150 and 300 mM of NaCl) with different plant growth regulators (PGR), indole-3-acetic acid (200 ppm IAA) and/or Kinetin (200 ppm KIN). The pre-soaking effects of two plant hormones, IAA and KIN on wheat grains exposed to moderate and high NaCl levels were investigated. According to the results, an increase in salinity caused a progressive decrease in shoot and root lengths, fresh and dry matter yields and the reduction in the compatible compounds chiefly under the highest level of NaCl. While proline content and malondialdehyde (MDA) were increased. Meanwhile, salinity triggered an imbalance in endogenous phytohormones, including KIN and IAA. In our experiment, the application of IAA or KIN or even the combination between them can control the growth processes, improve the salt tolerance of the plant and promote its growth.
几十年来，人们发现植物激素在调节植物生长方面起着主要作用。特别是当这些植物受到生物或非生物胁迫时。为了验证这一事实，本研究考察了盐度(0.0、150和300 mM NaCl)对不同植物生长调节剂(PGR)、吲哚-3-乙酸(200 ppm IAA)和/或Kinetin (200 ppm KIN)的影响。研究了IAA和KIN两种植物激素对中高NaCl胁迫下小麦籽粒的预浸泡效果。结果表明，盐度的增加导致茎长、根长、鲜物质和干物质产量以及相容性化合物的减少，主要是在最高NaCl水平下发生的。脯氨酸含量和丙二醛(MDA)含量升高。同时，盐度导致植物内源激素失调，包括KIN和IAA。在我们的试验中，IAA或KIN的施用甚至两者的组合都可以控制植株的生长过程，提高植株的耐盐性，促进植株的生长。
{"title":"EFFECT OF SALINITY AND PRIMING WITH PHYTOHORMONES ON GROWTH AND SOME PHYSIOLOGICAL PARAMETERS ON WHEAT PLANTS.","authors":"N. Youssef, A. Ismail, Naglaa Adly Hussien","doi":"10.21608/aunj.2020.220865","DOIUrl":"https://doi.org/10.21608/aunj.2020.220865","url":null,"abstract":"It was found for decades that phytohormones have a prime role in modifying the plant growth. Especially, when these plants are exposed to biotic or abiotic stress. Verify this fact, this study examined the effect of salinity (0.0, 150 and 300 mM of NaCl) with different plant growth regulators (PGR), indole-3-acetic acid (200 ppm IAA) and/or Kinetin (200 ppm KIN). The pre-soaking effects of two plant hormones, IAA and KIN on wheat grains exposed to moderate and high NaCl levels were investigated. According to the results, an increase in salinity caused a progressive decrease in shoot and root lengths, fresh and dry matter yields and the reduction in the compatible compounds chiefly under the highest level of NaCl. While proline content and malondialdehyde (MDA) were increased. Meanwhile, salinity triggered an imbalance in endogenous phytohormones, including KIN and IAA. In our experiment, the application of IAA or KIN or even the combination between them can control the growth processes, improve the salt tolerance of the plant and promote its growth.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83092472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220862
S. Mahgoub, A. Nassar, H. Omar, Amany M. A. Osman
Aflatoxins (AFs) are chemically secondary metabolites produced by Aspergillus, Penicillium and Fusarium genera. It has adverse effects on humans and animals health due to inhibition of macromolecule synthesis. The current research investigate the pathological and biochemical changes in lymph follicles of female rats exposed to aflatoxin B1 (AFB1) for one month and the efficacy of isolated bradykinin potentiating factor (BPF) from cobra snake venom, butylated hydroxy toluene (BHT) and oltipraz (OPZ) to ameliorate those changes. Aflatoxicosis cause significant increase of lipid peroxidation (LPO) and nitric oxide (NO) in addition to significant decrease in the level of total thiols, glutathione (GSH) and the activities of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Moreover, AFB1 caused Safaa A. Mahgoub et al. 27 http://www.aun.edu.eg E-mail: president@aun.edu.eg histopathological changes in lymph follicles represented by depletion of the lymphoid cells. Treatment of aflatoxicosed rats with BPF, BHT or OPZ resulting in amelioration of the oxidative stress markers and improvement in the histological structure of lymph follicles represented by increase of lymphoid cell population with presence of mast cells and collagen bundle. In conclusion BPF, BHT or OPZ ameliorate the aflatoxicosis with priority for the BPF.
黄曲霉毒素(AFs)是由曲霉、青霉和镰刀菌属产生的化学次生代谢物。由于抑制大分子合成，对人类和动物的健康有不良影响。本研究观察了雌性大鼠暴露于黄曲霉毒素B1 (AFB1)一个月后淋巴滤泡的病理生化变化，以及眼镜蛇蛇毒中分离的缓动肽增强因子(BPF)、丁基羟基甲苯(BHT)和奥替praz (OPZ)对这些变化的改善作用。黄曲霉中毒引起脂肪过氧化(LPO)和一氧化氮(NO)显著升高，总硫醇、谷胱甘肽(GSH)水平显著降低，谷胱甘肽过氧化物酶(GPx)和谷胱甘肽s转移酶(GST)活性显著降低。此外，AFB1引起Safaa A. Mahgoub等人27 http://www.aun.edu.eg E-mail: president@aun.edu.eg淋巴滤泡的组织病理学改变，表现为淋巴样细胞的耗损。用BPF、BHT或OPZ治疗黄曲霉中毒大鼠，可改善氧化应激标志物，改善淋巴滤泡的组织学结构，表现为淋巴样细胞群增加，肥大细胞和胶原束存在。结论BPF、BHT或OPZ对黄曲霉中毒的改善作用以BPF为主。
{"title":"Ameliorative effects of bradykinin potentiating factor, butylated hydroxy toluene and oltipraz on biochemical and histological structure of lymphoid organs in aflatoxicated female rats","authors":"S. Mahgoub, A. Nassar, H. Omar, Amany M. A. Osman","doi":"10.21608/aunj.2020.220862","DOIUrl":"https://doi.org/10.21608/aunj.2020.220862","url":null,"abstract":"Aflatoxins (AFs) are chemically secondary metabolites produced by Aspergillus, Penicillium and Fusarium genera. It has adverse effects on humans and animals health due to inhibition of macromolecule synthesis. The current research investigate the pathological and biochemical changes in lymph follicles of female rats exposed to aflatoxin B1 (AFB1) for one month and the efficacy of isolated bradykinin potentiating factor (BPF) from cobra snake venom, butylated hydroxy toluene (BHT) and oltipraz (OPZ) to ameliorate those changes. Aflatoxicosis cause significant increase of lipid peroxidation (LPO) and nitric oxide (NO) in addition to significant decrease in the level of total thiols, glutathione (GSH) and the activities of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Moreover, AFB1 caused Safaa A. Mahgoub et al. 27 http://www.aun.edu.eg E-mail: president@aun.edu.eg histopathological changes in lymph follicles represented by depletion of the lymphoid cells. Treatment of aflatoxicosed rats with BPF, BHT or OPZ resulting in amelioration of the oxidative stress markers and improvement in the histological structure of lymph follicles represented by increase of lymphoid cell population with presence of mast cells and collagen bundle. In conclusion BPF, BHT or OPZ ameliorate the aflatoxicosis with priority for the BPF.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80418757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/aunj.2020.220863
S. Hamid, M. A. A. El-Rus, Y. A. E. Kazzaz, A. Khudeir
The Mudargag area, 740 km 2 builds up two Neoproterozoic tectono-stratigraphic units separated by a NW trending subhorizontal thrust plane along which deformed rocks from both units are confined. The lower infrastructural unit is represented by the northern dome "A" of Wadi Hafafit culmination which forms a macroscopic ovalshaped asymmetrical antifromal domal structure with a fold axis extends over 10 km in a NW-SE direction. It is differentiated into four structural subunits composed of interlayered sequences, mainly of para-granite gneisses, psammitic gneisses and paraamphibolites with medium to high-grade metamorphic facies. Granite gneisses and psammitic gneisses dominate the lower and the upper subunits, respectively, paraamphibolites dominate the second subunit, whereas the third subunit has subequal proportions of psammitic gneisses and amphibolites. The upper suprastructural unit is represented by Shait ophiolitic mélange domain (SOMD), which comprises deformed normally faulted thin-skinned napped sequence of low-grade metamorphosed volcanosedimentary island-arc type assemblages incorporating dismembered ophiolite and partially carbonated serpentinite fragments. Petrographic examinations carried out on both dome "A" and SOMD show distinct evidences of polyphase deformation (D1-D3) in addition to tow metamorphic events (M1-M2). Field data revealed that the exhumation of the Hafafit culmination domes to which the present dome "A" is considered and the large-scale crustal extension of SOMD run within a left-lateral dominated transpressional wrenching of Najd Shear System.
{"title":"NEOPROTEROZOIC TECTONO-METAMORPHIC EVOLUTION OF HAFAFIT DOME \"A\" AND THE ABUTTING SHAIT OPHIOLITIC MÉLANGE DOMAIN AT GABEL MUDARGAG AREA, CENTRAL EASTERN DESERT, EGYPT","authors":"S. Hamid, M. A. A. El-Rus, Y. A. E. Kazzaz, A. Khudeir","doi":"10.21608/aunj.2020.220863","DOIUrl":"https://doi.org/10.21608/aunj.2020.220863","url":null,"abstract":"The Mudargag area, 740 km 2 builds up two Neoproterozoic tectono-stratigraphic units separated by a NW trending subhorizontal thrust plane along which deformed rocks from both units are confined. The lower infrastructural unit is represented by the northern dome \"A\" of Wadi Hafafit culmination which forms a macroscopic ovalshaped asymmetrical antifromal domal structure with a fold axis extends over 10 km in a NW-SE direction. It is differentiated into four structural subunits composed of interlayered sequences, mainly of para-granite gneisses, psammitic gneisses and paraamphibolites with medium to high-grade metamorphic facies. Granite gneisses and psammitic gneisses dominate the lower and the upper subunits, respectively, paraamphibolites dominate the second subunit, whereas the third subunit has subequal proportions of psammitic gneisses and amphibolites. The upper suprastructural unit is represented by Shait ophiolitic mélange domain (SOMD), which comprises deformed normally faulted thin-skinned napped sequence of low-grade metamorphosed volcanosedimentary island-arc type assemblages incorporating dismembered ophiolite and partially carbonated serpentinite fragments. Petrographic examinations carried out on both dome \"A\" and SOMD show distinct evidences of polyphase deformation (D1-D3) in addition to tow metamorphic events (M1-M2). Field data revealed that the exhumation of the Hafafit culmination domes to which the present dome \"A\" is considered and the large-scale crustal extension of SOMD run within a left-lateral dominated transpressional wrenching of Najd Shear System.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74755040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：