Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.138653.1011
M. Atia, Ebtehal Sallam, H. Abdel-Tawab, A. Alghriany
Background: Stem cells (SCs) from adipose tissue are being studied as part of regenerative medicine and used in vitro tissue engineering. Aim: The study goal was to highlight the modifications of fragmented adipose mesenchymal stem cells (AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7) by inducing cell death (either apoptosis or necrosis). Materials and Methods: This study explains the methods of isolating, identifying, and characterization of fragmented frozen AD-MSCs. Also, investigate the phenotype of AD-MSCs culturing cells obtained in Passage 3 using CD105 & CD90 as positive markers and CD45 as a stem cells preferentially
{"title":"The Role of Fragmented Adipose Mesenchymal Stem Cells in Vitro Therapy for Human Hepatoma Cell Line Growth","authors":"M. Atia, Ebtehal Sallam, H. Abdel-Tawab, A. Alghriany","doi":"10.21608/aunj.2022.138653.1011","DOIUrl":"https://doi.org/10.21608/aunj.2022.138653.1011","url":null,"abstract":"Background: Stem cells (SCs) from adipose tissue are being studied as part of regenerative medicine and used in vitro tissue engineering. Aim: The study goal was to highlight the modifications of fragmented adipose mesenchymal stem cells (AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7) by inducing cell death (either apoptosis or necrosis). Materials and Methods: This study explains the methods of isolating, identifying, and characterization of fragmented frozen AD-MSCs. Also, investigate the phenotype of AD-MSCs culturing cells obtained in Passage 3 using CD105 & CD90 as positive markers and CD45 as a stem cells preferentially","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87767091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.139022.1013
Abo Bakr Abdel Shakor, A. Alghriany, Hanem Abdel twab, E. Ahmed
DNA-protein cross-links and thymidine dimer are special types of DNA damages that formed when DNA exposed to many inducers as UV irradiations. Anthocyanins are members of phytochemicals found in many plants including Hibiscus sabdariffa. It was reported that anthocyanins protect DNA from UV-induced damages. Here we investigate the role of autophagy induced by anthocyanins in protection of UVB-induced DNA-protein cross-links and thymidine dimer in HepG2 cells . Cells were treated either with anthocyanins, Rapamycin, or combination of anthocyanins and Bafilomycin A1 or anthocyanins and 3-methyladenine for 24 h and then subjected to 0.36 J/cm 2 UVB irradiation. The results revealed that anthocyanins induce autophagy formation as detected by acridine orange pH dependent green to red (G/R) color shifting. To investigate the role of anthocyanins-induced autophagy on DNA-protein cross-links and thymidine dimer formation after UVB exposure, electrophoresis of protein covalently linked to DNA or thymidine dimer dot plot analysis of DNA were carried out. It was found that anthocyanins treated cells have reduced DNA-protein cross-links and thymidine dimer compared with the anthocyanins untreated cells. methyladenine together with anthocyanins significantly increased the DNA-protein cross-links and thymidine dimer formation compared with anthocyanins treated cells. Also, Rapamycin treatment alone protects cells from DNA-protein cross-links and thymidine dimer formation which confirm the role of autophagy in protection of cells from UVB-induced DNA-protein cross-links and thymidine dimer formation.
{"title":"Role of autophagy induced by anthocyanins in protection of DNA damages caused by UVB irradiation in HepG2 cells","authors":"Abo Bakr Abdel Shakor, A. Alghriany, Hanem Abdel twab, E. Ahmed","doi":"10.21608/aunj.2022.139022.1013","DOIUrl":"https://doi.org/10.21608/aunj.2022.139022.1013","url":null,"abstract":"DNA-protein cross-links and thymidine dimer are special types of DNA damages that formed when DNA exposed to many inducers as UV irradiations. Anthocyanins are members of phytochemicals found in many plants including Hibiscus sabdariffa. It was reported that anthocyanins protect DNA from UV-induced damages. Here we investigate the role of autophagy induced by anthocyanins in protection of UVB-induced DNA-protein cross-links and thymidine dimer in HepG2 cells . Cells were treated either with anthocyanins, Rapamycin, or combination of anthocyanins and Bafilomycin A1 or anthocyanins and 3-methyladenine for 24 h and then subjected to 0.36 J/cm 2 UVB irradiation. The results revealed that anthocyanins induce autophagy formation as detected by acridine orange pH dependent green to red (G/R) color shifting. To investigate the role of anthocyanins-induced autophagy on DNA-protein cross-links and thymidine dimer formation after UVB exposure, electrophoresis of protein covalently linked to DNA or thymidine dimer dot plot analysis of DNA were carried out. It was found that anthocyanins treated cells have reduced DNA-protein cross-links and thymidine dimer compared with the anthocyanins untreated cells. methyladenine together with anthocyanins significantly increased the DNA-protein cross-links and thymidine dimer formation compared with anthocyanins treated cells. Also, Rapamycin treatment alone protects cells from DNA-protein cross-links and thymidine dimer formation which confirm the role of autophagy in protection of cells from UVB-induced DNA-protein cross-links and thymidine dimer formation.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"137 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89355283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.139175.1015
M. Eraky, G. Abdelaal, A. El-Haddad
{"title":"Detection of Depth and Location of Subsurface Utilities Using Smart Phone Magnetic Application: A Case Study at Assiut University, Egypt","authors":"M. Eraky, G. Abdelaal, A. El-Haddad","doi":"10.21608/aunj.2022.139175.1015","DOIUrl":"https://doi.org/10.21608/aunj.2022.139175.1015","url":null,"abstract":"","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78977984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.139368.1016
Reda A. Ali, M. Atia, ola Aborehab, D. Mostafa
Introduction: is a standout amongst the most lethal metals derived from the natural environment. The major reason for human arsenic toxicity is tainting of drinking water. Arsenic trioxide is inorganic arsenic that is classified by the US Environmental Protection Agency as a known human carcinogen. Aim: Illustration of morphological and histological effects of arsenic trioxide on mice offspring. Methods: Adult female albino mice were divided into five groups: Control, negative control treated with Hcl (arsenic trioxide solvent), and three groups daily treated orally with different doses of arsenic trioxide (0.3, 0.7 and 1 mg/kg). Offspring of 21 day old mice were morphologically examined, weighed and morphometric measurements were carried out. Liver and kidney were histopathologically examined. Results: Data showed a significant decrease in body weight and crown rump length in the arsenic high dose treated group and a significant
{"title":"Morphological and histological changes induced by arsenic trioxide in mice offspring","authors":"Reda A. Ali, M. Atia, ola Aborehab, D. Mostafa","doi":"10.21608/aunj.2022.139368.1016","DOIUrl":"https://doi.org/10.21608/aunj.2022.139368.1016","url":null,"abstract":"Introduction: is a standout amongst the most lethal metals derived from the natural environment. The major reason for human arsenic toxicity is tainting of drinking water. Arsenic trioxide is inorganic arsenic that is classified by the US Environmental Protection Agency as a known human carcinogen. Aim: Illustration of morphological and histological effects of arsenic trioxide on mice offspring. Methods: Adult female albino mice were divided into five groups: Control, negative control treated with Hcl (arsenic trioxide solvent), and three groups daily treated orally with different doses of arsenic trioxide (0.3, 0.7 and 1 mg/kg). Offspring of 21 day old mice were morphologically examined, weighed and morphometric measurements were carried out. Liver and kidney were histopathologically examined. Results: Data showed a significant decrease in body weight and crown rump length in the arsenic high dose treated group and a significant","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80561553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.139639.1022
Marwa M. Thabet
this study, the impact of zinc oxide nanoparticles (ZnONPs) was addressed in the larval stages of Culex mosquito 3 days post treatment. Furthermore, the treated larvae were allowed to recover from the particle-effect for additional 3 and 6 days to address whether the particle impact is sustainable or temporary. To this end, a sub lethal concentration (LC30) of ZnONPs was used to address their impact on the tissues of the alimentary canal and Malpighian tubules of Culex larvae. The treated larvae suffered negative signs on foregut, midgut , hindgut and Malpighian tubule epithelia which appeared in the form of columnar cell vaculation and damage of the microvilli. In the meantime, the larvae which allowed recovering for 3 and 6 days didn’t show signs of improvement in the alimentary canal and Malpighian tubules suggesting sustainable impact of the particles on the larval tissues. Future work will be directed toward ultra-structural studies in the gut cells. This study suggests that ZnONPs can be used as promising tools in controlling mosquito larvae.
{"title":"Histological abnormalities in the alimentary canal and Malpighian tubules of Culex sp. mosquitoes larvae treated with zinc oxide nanoparticles","authors":"Marwa M. Thabet","doi":"10.21608/aunj.2022.139639.1022","DOIUrl":"https://doi.org/10.21608/aunj.2022.139639.1022","url":null,"abstract":"this study, the impact of zinc oxide nanoparticles (ZnONPs) was addressed in the larval stages of Culex mosquito 3 days post treatment. Furthermore, the treated larvae were allowed to recover from the particle-effect for additional 3 and 6 days to address whether the particle impact is sustainable or temporary. To this end, a sub lethal concentration (LC30) of ZnONPs was used to address their impact on the tissues of the alimentary canal and Malpighian tubules of Culex larvae. The treated larvae suffered negative signs on foregut, midgut , hindgut and Malpighian tubule epithelia which appeared in the form of columnar cell vaculation and damage of the microvilli. In the meantime, the larvae which allowed recovering for 3 and 6 days didn’t show signs of improvement in the alimentary canal and Malpighian tubules suggesting sustainable impact of the particles on the larval tissues. Future work will be directed toward ultra-structural studies in the gut cells. This study suggests that ZnONPs can be used as promising tools in controlling mosquito larvae.","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75851881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.140201.1026
Shimaa Said, Wafaa Mohamed, Gehad N. Aboulnasr, Z. Bakr, Naser A. Elshimy
In the past decades, nanotechnology has the use of nanoparticles (ENP) in a wide variety of fields. Metal nanoparticles (NPs) it was a source of interest for many environmental studies concerned with the study of toxicology since the early 2000s. Accumulated Nanomaterials in the soil through various activities such as sewage, atmospheric sedimentation, agrochemicals, and soil remediation[1]. Soil has been indicated pollutants [2]. The global productivity of Ag NPs ranges between 500 ton/year and 1000 ton/year [3, 4]. Ag NPs are used as an antimicrobial component in a lot of commercial products such ARTICLE INFO ABSTRACT Article History: Received: 2022-05-23 Accepted: 2022-05-26 Online: 2022-08-14 _______________
{"title":"Impact of Ag NPs and AgNO3 on the histopathological studies of land slug (Lehmannia nyctelia) inhabiting Assiut city, Egypt","authors":"Shimaa Said, Wafaa Mohamed, Gehad N. Aboulnasr, Z. Bakr, Naser A. Elshimy","doi":"10.21608/aunj.2022.140201.1026","DOIUrl":"https://doi.org/10.21608/aunj.2022.140201.1026","url":null,"abstract":"In the past decades, nanotechnology has the use of nanoparticles (ENP) in a wide variety of fields. Metal nanoparticles (NPs) it was a source of interest for many environmental studies concerned with the study of toxicology since the early 2000s. Accumulated Nanomaterials in the soil through various activities such as sewage, atmospheric sedimentation, agrochemicals, and soil remediation[1]. Soil has been indicated pollutants [2]. The global productivity of Ag NPs ranges between 500 ton/year and 1000 ton/year [3, 4]. Ag NPs are used as an antimicrobial component in a lot of commercial products such ARTICLE INFO ABSTRACT Article History: Received: 2022-05-23 Accepted: 2022-05-26 Online: 2022-08-14 _______________","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81622898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21608/aunj.2022.139563.1018
M. Alfons, G. Badr, A. Harabawy, M. Al-Salahy, A. Ibrahim
The purpose of this study was to look into the ability of a propolis-supplemented diet on some haematological indices and histological alterations in the gill, kidney, and testes of catfish ( Clarias gariepinus ) to cope with heat
{"title":"Amelioration effect of propolis supplementation on haematological indices and histopathological alterations of Clarias gariepinus during heat stress","authors":"M. Alfons, G. Badr, A. Harabawy, M. Al-Salahy, A. Ibrahim","doi":"10.21608/aunj.2022.139563.1018","DOIUrl":"https://doi.org/10.21608/aunj.2022.139563.1018","url":null,"abstract":"The purpose of this study was to look into the ability of a propolis-supplemented diet on some haematological indices and histological alterations in the gill, kidney, and testes of catfish ( Clarias gariepinus ) to cope with heat","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83379692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.21608/aunj.2022.132015.1007
M. Salah, Gamal Badr, H. Hetta, W. Khalifa, A. Shoreit
Diabetic foot ulcer (DFU) is the most serious diabetic complication. Gangrene is causing by the successive bacterial infection invading diabetic wounds and may lead to limb amputation for the diabetic patient. The bacteria inhabiting the wound exhibit the ability to forming biofilm, which is a protected mode of growth that allows
{"title":"Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds","authors":"M. Salah, Gamal Badr, H. Hetta, W. Khalifa, A. Shoreit","doi":"10.21608/aunj.2022.132015.1007","DOIUrl":"https://doi.org/10.21608/aunj.2022.132015.1007","url":null,"abstract":"Diabetic foot ulcer (DFU) is the most serious diabetic complication. Gangrene is causing by the successive bacterial infection invading diabetic wounds and may lead to limb amputation for the diabetic patient. The bacteria inhabiting the wound exhibit the ability to forming biofilm, which is a protected mode of growth that allows","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81811138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.21608/aunj.2022.120568.1001
Marwa E Abdelmageed
All spiders having different types of hairs. The spider's body hair and spines are hollow and highly sensitive to touch
所有的蜘蛛都有不同种类的毛发。蜘蛛的体毛和刺是中空的，对触摸非常敏感
{"title":"Morphological studies on sense organs of the adult female of the true spider Cheiracanthium isiacum (Araneae: Cheiracanthiidae) at Assiut Governorate, Egypt.","authors":"Marwa E Abdelmageed","doi":"10.21608/aunj.2022.120568.1001","DOIUrl":"https://doi.org/10.21608/aunj.2022.120568.1001","url":null,"abstract":"All spiders having different types of hairs. The spider's body hair and spines are hollow and highly sensitive to touch","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78960285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.21608/aunj.2022.234650
M. M. Hassan, A. Shehata, M. S. Abdel-Daym
In this paper, we apply the extended Kudryashov method to construct some new exact solitary wave solutions of three important physical models, Schamel-nonlinear
本文应用扩展Kudryashov方法构造了三种重要的schamel -非线性物理模型的精确孤立波解
{"title":"Exact Solutions to a Class of Schamel Nonlinear Equations Modeling Dust Ion-acoustic Waves in Plasma","authors":"M. M. Hassan, A. Shehata, M. S. Abdel-Daym","doi":"10.21608/aunj.2022.234650","DOIUrl":"https://doi.org/10.21608/aunj.2022.234650","url":null,"abstract":"In this paper, we apply the extended Kudryashov method to construct some new exact solitary wave solutions of three important physical models, Schamel-nonlinear","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91420690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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