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Effect of hydro-alcoholic extract of Panax ginseng and Ampicillin treatment in an animal model of Listeria monocytogenes-induced endocarditis. 人参水醇提取物和氨苄青霉素治疗单核细胞增多性李斯特菌诱导的心内膜炎动物模型的效果。
IF 2.2 Q2 Medicine Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.21227
Seyed Zanyar Athari, Zahra Karamouz, Mir Alireza Nourazar, Yousef Doustar, Younes Anzabi

Objective: Endocarditis is a rare but serious infection caused by Listeria monocytogenes. Panax ginseng demonstrated multiple immunomodulatory effects in earlier studies. Ampicillin is known as an effective antibiotic in the treatment of this disease. Therefore, this study aimed to evaluate the effect of hydro-alcoholic extract of P. ginseng and ampicillin treatment in an animal model of Listeria monocytogenes-induced endocarditis.

Materials and methods: Thirty mice, 5-7 weeks old, were randomly divided into five groups (n=6) including Healthy Control, Infected, Ampicillin (20 mg/kg, subcutaneous) treatment, Ginseng (0.025 mg/kg, intraperitoneal) treatment, and Ginseng (0.025 mg/kg, intraperitoneal) +Ampicillin (15 mg/kg, subcutaneous) treatment groups. The concentration of cytokines in heart tissue, such as IL-1 (interleukine-1), IL-6, IL-8, and TNF-α (Tumor Necrosis Factor-α), was measured. Histopathological changes were evaluated in heart tissues.

Results: The levels of cytokines were significantly decreased in the Ampicillin+Ginseng treated group compared to the other experimental groups. Microscopically, pathologic changes in heart tissue were concomitant with biochemical findings, which in the infected group, neutrophils and mononuclear cells infiltration in endocardial tissue, myocardial cell necrosis, and edema were detectable. The Ampicillin+Ginseng group showed no significant changes compared to the normal control group.

Conclusion: This study showed that ginseng hydro-alcoholic extract plus ampicillin has better efficacy than the extract or antibiotic alone against experimental endocarditis caused by Listeriosis.

目的:心内膜炎是由单核细胞增多性李斯特菌引起的一种罕见但严重的感染。人参在早期的研究中显示出多种免疫调节作用。众所周知,氨苄青霉素是治疗这种疾病的有效抗生素。因此,本研究旨在评估人参水醇提取物和氨苄青霉素治疗单核细胞增多性李斯特菌诱导的心内膜炎动物模型的效果。材料和方法:30只5-7周龄的小鼠随机分为5组(n=6),包括健康对照组、感染组、氨苄青霉素(20mg/kg,皮下)治疗组、人参(0.025mg/kg,腹膜内)治疗组和人参(0.025 mg/kg,腹腔内)+氨苄青霉素组(15mg/kg,皮下)。测量心脏组织中细胞因子的浓度,如IL-1(白细胞介素-1)、IL-6、IL-8和TNF-α(肿瘤坏死因子-α)。评估心脏组织的组织病理学变化。结果:与其他实验组相比,氨苄青霉素+人参治疗组的细胞因子水平显著降低。显微镜下,心脏组织的病理变化伴随着生化结果,在感染组中,可以检测到心内膜组织中的中性粒细胞和单核细胞浸润、心肌细胞坏死和水肿。与正常对照组相比,氨苄青霉素+人参组没有显示出显著的变化。结论:人参水醇提取物联合氨苄青霉素对李斯特菌病引起的实验性心内膜炎有较好的疗效。
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引用次数: 0
Evaluating the effect of an Iranian traditional medicine-based herbal candy on body composition and appetite in overweight and obese adults: A preliminary study. 评估伊朗传统药物草药糖果对超重和肥胖成年人身体成分和食欲的影响:一项初步研究。
IF 2.2 Q2 Medicine Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.21314
Maryam Golzar, Effat Saghi, Hassan Rakhshandeh, Zahra Dehnavi, Ali Jafarzadeh Esfehani, Mohsen Nematy

Objective: Obesity is an important public health concern in the world. Considering the limited medications and their side effects and lack of a known effective way to reduce appetite, traditional herbs have been considered a complementary treatment for obesity. Therefore, this study was conducted to evaluate the effect of an Iranian Traditional Medicine (ITM)-based herbal candy on body composition and appetite in obese and overweight adults.

Materials and methods: In this preliminary study that conducted in nutrition clinic of Ghaem Hospital of Mashhad, participants were selected from overweight and obese people and randomly assigned to either groups. Participants in the intervention group received herbal candy which contained a combination of Portulaca oleracea, Plantago psyllium, and peanut oil, while participants in the control group received placebo candy for 8 weeks. The primary (appetite response, and weight changes) and secondary (body mass index (BMI), anthropometric parameters, blood pressure, and laboratory variables) outcomes were collected at baseline and during the intervention.

Results: Fifty participants between the age of 18 and 65 years old were included in this study. Herbal candy resulted in a greater reduction in mean weight and BMI compared to placebo (p<0.001). Mean of hunger, satiety, and eating capacity indicators decreased more significantly at all three intervals (30 min after herbal candy, 1 hour after meal and 2 hours after meal) at lunch and dinner meal in the intervention group compared to the control group (p<0.05).

Conclusion: A combination of produced herbal candy at a dose of 4 g (2 pcs), given half an hour before each meal for 8 weeks, can be effective in reducing weight and appetite in obese and overweight people.

目的:肥胖是世界上一个重要的公共卫生问题。考虑到药物及其副作用有限,而且缺乏已知的有效减肥方法,传统草药被认为是治疗肥胖的补充疗法。因此,本研究旨在评估一种基于伊朗传统医学(ITM)的草药糖果对肥胖和超重成年人身体成分和食欲的影响。材料和方法:在马什哈德盖姆医院营养诊所进行的这项初步研究中,参与者从超重和肥胖人群中选择,并随机分为两组。干预组的参与者接受了含有马齿苋、车前子和花生油的草药糖果,而对照组的参与者则接受了为期8周的安慰剂糖果。在基线和干预期间收集主要(食欲反应和体重变化)和次要(体重指数(BMI)、人体测量参数、血压和实验室变量)结果。结果:50名年龄在18岁至65岁之间的参与者被纳入本研究。与安慰剂相比,草药糖能显著降低平均体重和BMI(结论:在8周内,每餐前半小时服用4克(2片)的草药糖,可以有效降低肥胖和超重人群的体重和食欲。
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引用次数: 0
Neuroprotective effects of sesamol against LPS-induced spatial learning and memory deficits are mediated via anti-inflammatory and antioxidant activities in the rat brain. 芝麻酚对LPS诱导的空间学习和记忆缺陷的神经保护作用是通过大鼠大脑中的抗炎和抗氧化活性介导的。
IF 2.2 Q2 Medicine Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.21403
Zahra Beheshtimanesh, Ziba Rajaei

Objective: Sesamol is a phenolic lignan extracted from sesame seeds, and it possesses anti-inflammatory and antioxidant activities. Lipopolysaccharide (LPS) is known to produce neuroinflammatory responses and memory impairment. The current study aimed to investigate the protective influence of sesamol against LPS-mediated neuroinflammation and memory impairment.

Materials and methods: Sesamol (10 and 50 mg/kg) was injected to Wistar rats for two weeks. Then, animals received LPS injection (1 mg/kg) for five days, while treatment with sesamol was performed 30 min before LPS injection. Spatial learning and memory were assessed by the Morris water maze (MWM), two hours after LPS injection on days 15-19. Biochemical assessments were performed after the end of behavioral experiments.

Results: LPS-administered rats showed spatial learning and memory deficits, since they spent more time in the MWM to find the hidden platform and less time in the target quadrant. Besides these behavioral changes, tumor necrosis factor-α (TNF-α) and lipid peroxidation levels were increased, while total thiol level was decreased in the hippocampus and/or cerebral cortex. In addition, sesamol treatment (50 mg/kg) for three weeks decreased the escape latency and increased the time on probe trial. Sesamol also reduced lipid peroxidation and TNF-α level, while enhanced total thiol level in the brain of LPS-exposed rats.

Conclusion: Supplementation of sesamol attenuated learning and memory impairments in LPS-treated rats via antioxidative and anti-inflammatory activities in the rat brain.

目的:芝麻酚是从芝麻中提取的酚类木脂素,具有抗炎、抗氧化等活性。众所周知,脂多糖(LPS)会产生神经炎症反应和记忆障碍。本研究旨在探讨芝麻酚对LPS介导的神经炎症和记忆障碍的保护作用。材料和方法:给Wistar大鼠注射芝麻酚(10和50mg/kg),持续两周。然后,动物接受LPS注射(1mg/kg)5天,同时在LPS注射前30分钟进行芝麻酚处理。在第15-19天注射LPS两小时后,通过Morris水迷宫(MWM)评估空间学习和记忆。在行为实验结束后进行生化评估。结果:LPS给药的大鼠表现出空间学习和记忆缺陷,因为它们在MWM中花费更多的时间来寻找隐藏的平台,而在目标象限中花费更少的时间。除这些行为变化外,海马和/或大脑皮层的肿瘤坏死因子-α(TNF-α)和脂质过氧化水平升高,而总硫醇水平降低。此外,三周的芝麻酚治疗(50mg/kg)降低了逃逸潜伏期,增加了探针试验的时间。芝麻酚还降低了LPS暴露大鼠脑内脂质过氧化和TNF-α水平,同时提高了总硫醇水平。结论:补充芝麻酚通过在大鼠大脑中的抗氧化和抗炎活性减轻LPS处理的大鼠的学习和记忆障碍。
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引用次数: 1
Therapeutic effects of herbal medicines in different types of retinopathies: A systematic review. 草药对不同类型视网膜病变的治疗作用:系统综述。
IF 2.2 Q2 Medicine Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.62423.2977
Negin Ansari-Mohseni, Adel Ghorani-Azam, Seyed Ahmad Mohajeri

Objective: Retinopathy is an ocular manifestation of systemic diseases such as diabetes and vascular diseases. Herbal drugs have been considered as an effective therapeutic option with minimal side effects for the treatment of retinopathy by reducing the symptoms and improving visual acuity. The purpose of this systematic review was to collect studies on the effectiveness of medicinal plants in the treatment or prevention of retinopathy.

Materials and methods: A systematic literature search was performed in PubMed, Scopus, Google Scholar, and other databases in April 2021 using "herbal products" and "Retinopathy" with all their equivalent and similar terms. For this purpose, human clinical trials with the English language were included and articles with subject irrelevancy were excluded from further evaluation.

Results: Overall, 30 articles with 2324 patients were studied for possible effects of herbal therapy on retinopathy. From 30 included articles, different herbal products had been evaluated. Out of 30 selected articles, 11 articles were for the treatment of age-related macular degeneration (AMD), 14 articles covered patients with diabetic retinopathy, and the other five studies were for other retinal disorders. The outcomes in majority of the studies include changes in visual acuity (VA), fundus performance, best-corrected visual acuity (BCVA), central macular thickness (CMT), focal electroretinogram (fERG), supplements and adjuvant medications appeared to be more beneficial in patients with AMD and diabetic maculopathy.

Conclusion: Herbal therapy can be considered as a potential candidate in the adjuvant and complementary therapies of retinopathy. However, further studies are required to verify such efficiency.

目的:视网膜病变是糖尿病和血管疾病等全身性疾病的眼部表现。草药被认为是一种有效的治疗选择,通过减轻症状和提高视力来治疗视网膜病变,副作用最小。本系统综述的目的是收集有关药用植物治疗或预防视网膜病变有效性的研究。材料和方法:2021年4月,在PubMed、Scopus、Google Scholar和其他数据库中使用“草药产品”和“视网膜病变”及其所有等效和类似术语进行了系统的文献搜索。为此,纳入了英语的人类临床试验,并将与受试者无关的文章排除在进一步评估之外。结果:总共研究了30篇文章,涉及2324名患者,探讨了草药治疗视网膜病变的可能效果。从纳入的30篇文章中,对不同的草药产品进行了评估。在30篇精选文章中,11篇文章是关于年龄相关性黄斑变性(AMD)的治疗,14篇文章涉及糖尿病视网膜病变患者,其他5项研究涉及其他视网膜疾病。大多数研究的结果包括视力(VA)、眼底表现、最佳矫正视力(BCVA)、中央黄斑厚度(CMT)、局灶性视网膜电图(fERG)的变化,补充剂和辅助药物似乎对AMD和糖尿病黄斑病变患者更有益。结论:中药治疗可作为视网膜病变的辅助和补充治疗的潜在候选药物。然而,还需要进一步的研究来验证这种效率。
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引用次数: 1
Effect of Ajwa date pits powder (Phoenix dactylifera L.) on body composition, lipid profile and blood pressure in patients with hyperlipidemia: A randomized clinical trial. Ajwa枣核粉对高脂血症患者身体成分、血脂和血压的影响:一项随机临床试验。
IF 2.2 Q2 Medicine Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.21316
Parkha Riaz Nasrullah, Bismillah Sehar, Mahpara Safdar, Sadia Fatima, Falak Zeb, Jahan Shah, Atif Ali Khan Khalil, Bilal Ahmed, Ali Saad R Alsubaie, Muhammad Haidar Zaman

Objective: To evaluate the effect of Ajwa dates pit powder (ADP) on lipid profile, body composition and blood pressure in patients with hyperlipidemia.

Materials and methods: This randomized controlled clinical study was carried out on 40 patients with total cholesterol >200 mg/dl, triglycerides >150 mg/dl and BMI >25, of either sex, aged 30-50 years, who were recruited through written consent. The patients were divided into two groups (n=20 each): the ADP and the control group (CG). All patients received the doctor's prescribed class A statin (Rosuvastatin/ Atorvastatin) 10 mg/day, while 2.7 g ADP was given on daily basis before breakfast with lukewarm water for 40 days and the control group received the same amount of wheat flour. Body composition, blood pressure and lipid profile were determined at baseline, and after 20 and 40 days. Data were analyzed by using SPSS and GraphPad Prism.

Results: ADP significantly reduced body weight (p<0.001), BMI (p<0.001), fat mass, body fat percentage, visceral fat area and waist circumference compared to the control group. Similarly, ADP significantly (p=0.000) decreased the serum level of total cholesterol and low-density lipoprotein.

Conclusion: ADP may have the potential to improve dyslipidemia and obesity.

目的:评价阿杰瓦红枣核散对高脂血症患者血脂、身体成分及血压的影响。材料和方法:这项随机对照临床研究对40名总胆固醇>200 mg/dl、甘油三酯>150 mg/dl、BMI>25的患者进行,这些患者年龄在30-50岁之间,属于任何性别,通过书面同意招募。将患者分为两组(每组n=20):ADP组和对照组(CG)。所有患者均接受医生处方的A类他汀类药物(瑞舒伐他汀/阿托伐他汀)10mg/天,而每天早餐前用温水给予2.7g ADP,持续40天,对照组接受等量的小麦粉。在基线、20天和40天后测定身体成分、血压和脂质状况。结果:ADP能显著减轻体重(P结论:ADP可能具有改善血脂异常和肥胖的潜力。
{"title":"Effect of Ajwa date pits powder (<i>Phoenix dactylifera</i> L.) on body composition, lipid profile and blood pressure in patients with hyperlipidemia: A randomized clinical trial.","authors":"Parkha Riaz Nasrullah,&nbsp;Bismillah Sehar,&nbsp;Mahpara Safdar,&nbsp;Sadia Fatima,&nbsp;Falak Zeb,&nbsp;Jahan Shah,&nbsp;Atif Ali Khan Khalil,&nbsp;Bilal Ahmed,&nbsp;Ali Saad R Alsubaie,&nbsp;Muhammad Haidar Zaman","doi":"10.22038/AJP.2022.21316","DOIUrl":"10.22038/AJP.2022.21316","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of Ajwa dates pit powder (ADP) on lipid profile, body composition and blood pressure in patients with hyperlipidemia.</p><p><strong>Materials and methods: </strong>This randomized controlled clinical study was carried out on 40 patients with total cholesterol >200 mg/dl, triglycerides >150 mg/dl and BMI >25, of either sex, aged 30-50 years, who were recruited through written consent. The patients were divided into two groups (n=20 each): the ADP and the control group (CG). All patients received the doctor's prescribed class A statin (Rosuvastatin/ Atorvastatin) 10 mg/day, while 2.7 g ADP was given on daily basis before breakfast with lukewarm water for 40 days and the control group received the same amount of wheat flour. Body composition, blood pressure and lipid profile were determined at baseline, and after 20 and 40 days. Data were analyzed by using SPSS and GraphPad Prism.</p><p><strong>Results: </strong>ADP significantly reduced body weight (p<0.001), BMI (p<0.001), fat mass, body fat percentage, visceral fat area and waist circumference compared to the control group. Similarly, ADP significantly (p=0.000) decreased the serum level of total cholesterol and low-density lipoprotein.</p><p><strong>Conclusion: </strong>ADP may have the potential to improve dyslipidemia and obesity.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of crocin and losartan on TGF-β gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy. 番红花和氯沙坦对糖尿病肾病大鼠肾组织TGF-β基因表达和组织病理学的影响。
IF 2.2 Q2 Medicine Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.21414
Yaser Mohammadi, Mohammad Zangooei, Mahmoud Zardast, Morteza Mamashli, Azam Rezaei Farimani

Objective: Diabetic nephropathy is one of the most common microvascular complications of diabetes mellitus that finally leads to complete loss of kidney function. Therefore, this study aimed to evaluate the effect of crocin and losartan on TGF-β gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.

Materials and methods: Forty male Wistar rats were randomly divided into five groups (n=8): Untreated control, Diabetic (D), D + crocin, D + losartan, and D + losartan + crocin. Induction of diabetes was performed using streptozotocin (50 mg/kg/ Intraperitoneal injection). At the end of the eight-week period, the rats were sacrificed. Spectrophotometry measured serum glucose, urea, creatinine, and uric acid levels. Microalbumin and creatinine levels were measured in 24-hour urine. Real-time PCR was used to determine the relative expression of the TGF-β gene in kidney tissue. Renal tissue histopathology was also examined.

Results: The results showed that hyperglycemia increased biochemical factors associated with diabetes, TGF-β gene expression, and kidney damage. Separate treatment with crocin and losartan led to a decrease in renal function factors and TGF-β gene expression and improved kidney damage.

Conclusion: Our results showed that crocin could improve kidney function in diabetic conditions. In addition, we showed that crocin increases the effectiveness of losartan. Consequently, we suggest that crocin in combination with chemical drugs can be a potential therapeutic agent for diabetes and its complications. Nonetheless, human studies are needed to make firm findings.

目的:糖尿病肾病是糖尿病最常见的微血管并发症之一,最终导致肾功能完全丧失。因此,本研究旨在评估番红花和氯沙坦对糖尿病肾病大鼠模型肾组织TGF-β基因表达和组织病理学的影响。材料和方法:雄性Wistar大鼠40只,随机分为5组(n=8):未治疗对照组、糖尿病组(D)、D+克罗辛组、D+氯沙坦组和D+氯沙坦+克罗辛。使用链脲佐菌素(50mg/kg/腹膜内注射)诱导糖尿病。在八周结束时,处死大鼠。分光光度法测量血清葡萄糖、尿素、肌酸酐和尿酸水平。测量24小时尿液中的微量白蛋白和肌酐水平。实时聚合酶链式反应检测肾组织中TGF-β基因的相对表达。还检查了肾组织病理学。结果:高血糖增加了与糖尿病、TGF-β基因表达和肾损伤相关的生化因子。单独使用番红花苷和氯沙坦治疗可降低肾功能因子和TGF-β基因表达,改善肾损伤。结论:番红花苷可改善糖尿病患者的肾功能。此外,我们发现番红花苷能提高氯沙坦的疗效。因此,我们认为番红花苷与化学药物联合治疗糖尿病及其并发症可能是一种潜在的治疗剂。尽管如此,还是需要进行人类研究才能得出确切的结论。
{"title":"The effect of crocin and losartan on <i>TGF-β</i> gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.","authors":"Yaser Mohammadi,&nbsp;Mohammad Zangooei,&nbsp;Mahmoud Zardast,&nbsp;Morteza Mamashli,&nbsp;Azam Rezaei Farimani","doi":"10.22038/AJP.2022.21414","DOIUrl":"10.22038/AJP.2022.21414","url":null,"abstract":"<p><strong>Objective: </strong>Diabetic nephropathy is one of the most common microvascular complications of diabetes mellitus that finally leads to complete loss of kidney function. Therefore, this study aimed to evaluate the effect of crocin and losartan on <i>TGF-β</i> gene expression and histopathology of kidney tissue in a rat model of diabetic nephropathy.</p><p><strong>Materials and methods: </strong>Forty male Wistar rats were randomly divided into five groups (n=8): Untreated control, Diabetic (D), D + crocin, D + losartan, and D + losartan + crocin. Induction of diabetes was performed using streptozotocin (50 mg/kg/ Intraperitoneal injection). At the end of the eight-week period, the rats were sacrificed. Spectrophotometry measured serum glucose, urea, creatinine, and uric acid levels. Microalbumin and creatinine levels were measured in 24-hour urine. Real-time PCR was used to determine the relative expression of the <i>TGF-β</i> gene in kidney tissue. Renal tissue histopathology was also examined.</p><p><strong>Results: </strong>The results showed that hyperglycemia increased biochemical factors associated with diabetes, <i>TGF-β</i> gene expression, and kidney damage. Separate treatment with crocin and losartan led to a decrease in renal function factors and <i>TGF-β</i> gene expression and improved kidney damage.</p><p><strong>Conclusion: </strong>Our results showed that crocin could improve kidney function in diabetic conditions. In addition, we showed that crocin increases the effectiveness of losartan. Consequently, we suggest that crocin in combination with chemical drugs can be a potential therapeutic agent for diabetes and its complications. Nonetheless, human studies are needed to make firm findings.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9663231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The effect of curcumin-piperine on cardiometabolic, inflammatory and oxidative stress factors and macular vascular density in optical coherence tomography angiography (OCTA) in patients with non-proliferative diabetic retinopathy: Study protocol for a randomized, double-blind controlled trial. 光学相干断层扫描血管造影术(OCTA)中姜黄素-哌啶对非增殖性糖尿病视网膜病变患者心脏代谢、炎症和氧化应激因子以及黄斑血管密度的影响:一项随机、双盲对照试验的研究方案。
IF 1.9 Q3 CHEMISTRY, MEDICINAL Pub Date : 2023-03-01 DOI: 10.22038/AJP.2022.21512
Sepide Amini, Amirhossein Sahebkar, Alireza Dehghani, Bijan Iraj, Abdolreza Rezaeian-Ramsheh, Gholamreza Askari, Muhammed Majeed, Mohammad Bagherniya

Objective: Curcumin is a safe phytochemical with antioxidant, anti-inflammatory, antidiabetic, and lipid-lowering effects. This study aims to investigate the efficacy of curcumin-piperine in non-proliferative diabetic retinopathy.

Materials and methods: In this double-blind randomized trial, 60 diabetic retinopathy patients after meeting the inclusion criteria will be randomly assigned to two groups of curcumin-piperine supplementation (1000 mg per day for 12 weeks) or receiving placebo. The density of small blood vessels in the retina by optical coherence tomography angiography (OCTA), fasting blood glucose, triglyceride, renal indices (blood urea nitrogen and creatinine), high-sensitivity C-reactive protein, total antioxidant capacity, total oxidant status, body mass index, waist circumference, and weight will be measured.

Conclusion: If the beneficial effects of curcumin on diabetic retinopathy are observed, this safe, this natural and inexpensive herbal supplement can be considered a therapeutic solution in these patients.

目的:姜黄素是一种安全的植物化学物质,具有抗氧化、抗炎、抗糖尿病、降脂等作用。本研究旨在探讨姜黄素哌啶治疗非增殖性糖尿病视网膜病变的疗效。材料和方法:在这项双盲随机试验中,60名符合纳入标准的糖尿病视网膜病变患者将被随机分为两组,分别补充姜黄素-哌啶(每天1000mg,持续12周)或接受安慰剂治疗。通过光学相干断层扫描血管造影术(OCTA)测量视网膜中小血管的密度、空腹血糖、甘油三酯、肾脏指数(血尿素氮和肌酐)、高敏C反应蛋白、总抗氧化能力、总氧化剂状态、体重指数、腰围和体重。结论:如果观察到姜黄素对糖尿病视网膜病变的有益作用,这种安全、天然、廉价的草药补充剂可以被认为是这些患者的治疗方案。
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引用次数: 0
Nigella sativa supplementation and non-alcoholic fatty liver disease: A systematic review of clinical trials. Nigella sativa补充剂与非酒精性脂肪肝:临床试验的系统综述。
IF 2.2 Q2 Medicine Pub Date : 2023-01-01 DOI: 10.22038/AJP.2022.20060
Abbas Mohtashamian, Armin Ebrahimzadeh, Zahra Shamekhi, Nasrin Sharifi

Objective: Based on the results of previous studies, the effects of N. sativa on some of the non-alcoholic fatty liver disease's (NAFLD) biomarkers were positive; however, there were conflicting results regarding other variables. Therefore, the present systematic review of clinical trials was designed to clarify whether N. sativa effectively prevents the progression of NAFLD.

Materials and methods: A search of four databases (Scopus, PubMed, Medline, and Google scholar) was conducted to identify the clinical trials that assessed the effects of N. sativa supplementation on NAFLD. The outcome variables of interest were biomarkers of hepatic steatosis, liver enzymes, insulin resistance, and inflammation.

Results: Overall, four randomized clinical trials (RCTs) were included. In three studies, hepatic steatosis grade decreased significantly after N. sativa supplementation. Serum levels of liver enzymes reduced significantly in three of four included trials. In the only study that examined the effect of N. sativa on insulin resistance parameters, all variables related to this factor were significantly reduced. In two included studies that measured biomarkers of inflammation, the serum levels of tumor necrosis factor α (TNF-α), high-sensitive C-reactive protein (hs-CRP), and interleukin 6 (IL-6) decreased significantly after intaking N. sativa supplements.

Conclusion: Although the efficacy of N. sativa on liver enzymes and the grade of hepatic steatosis was reported in some of the included studies, more well-designed clinical trials are needed to determine the definitive effects of N. sativa on NAFLD. The present study provides suggestions that help to design future studies in this field.

目的:根据以往的研究结果,N.sativa对一些非酒精性脂肪肝(NAFLD)生物标志物的影响是积极的;然而,在其他变量方面存在着相互矛盾的结果。因此,本临床试验的系统综述旨在阐明N.sativa是否能有效预防NAFLD的进展。材料和方法:检索四个数据库(Scopus、PubMed、Medline和Google scholar),以确定评估补充N.sativas对NAFLD影响的临床试验。感兴趣的结果变量是肝脂肪变性、肝酶、胰岛素抵抗和炎症的生物标志物。结果:总共纳入了四项随机临床试验。在三项研究中,补充N.sativa后,肝脏脂肪变性程度显著降低。在四项纳入的试验中,有三项试验的血清肝酶水平显著降低。在唯一一项研究N.sativa对胰岛素抵抗参数的影响的研究中,与该因素相关的所有变量都显著减少。在两项测量炎症生物标志物的纳入研究中,服用N.sativa补充剂后,血清肿瘤坏死因子α(TNF-α)、高敏C反应蛋白(hs-CRP)和白细胞介素6(IL-6)水平显著下降。结论:尽管在一些纳入的研究中报道了N.sativa对肝酶的作用和肝脂肪变性的程度,但还需要更精心设计的临床试验来确定N.sativa对NAFLD的确切影响。本研究提供了有助于设计该领域未来研究的建议。
{"title":"<i>Nigella sativa</i> supplementation and non-alcoholic fatty liver disease: A systematic review of clinical trials.","authors":"Abbas Mohtashamian,&nbsp;Armin Ebrahimzadeh,&nbsp;Zahra Shamekhi,&nbsp;Nasrin Sharifi","doi":"10.22038/AJP.2022.20060","DOIUrl":"10.22038/AJP.2022.20060","url":null,"abstract":"<p><strong>Objective: </strong>Based on the results of previous studies, the effects of <i>N. sativa</i> on some of the non-alcoholic fatty liver disease's (NAFLD) biomarkers were positive; however, there were conflicting results regarding other variables. Therefore, the present systematic review of clinical trials was designed to clarify whether <i>N. sativa</i> effectively prevents the progression of NAFLD.</p><p><strong>Materials and methods: </strong>A search of four databases (Scopus, PubMed, Medline, and Google scholar) was conducted to identify the clinical trials that assessed the effects of <i>N. sativa</i> supplementation on NAFLD. The outcome variables of interest were biomarkers of hepatic steatosis, liver enzymes, insulin resistance, and inflammation.</p><p><strong>Results: </strong>Overall, four randomized clinical trials (RCTs) were included. In three studies, hepatic steatosis grade decreased significantly after <i>N. sativa</i> supplementation. Serum levels of liver enzymes reduced significantly in three of four included trials. In the only study that examined the effect of <i>N. sativa</i> on insulin resistance parameters, all variables related to this factor were significantly reduced. In two included studies that measured biomarkers of inflammation, the serum levels of tumor necrosis factor α (TNF-α), high-sensitive C-reactive protein (hs-CRP), and interleukin 6 (IL-6) decreased significantly after intaking <i>N. sativa</i> supplements.</p><p><strong>Conclusion: </strong>Although the efficacy of <i>N. sativa</i> on liver enzymes and the grade of hepatic steatosis was reported in some of the included studies, more well-designed clinical trials are needed to determine the definitive effects of <i>N. sativa</i> on NAFLD. The present study provides suggestions that help to design future studies in this field.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Protective effects of hydroalcoholic extract of Rosa canina L. fruit on cyclophosphamide-induced testicular toxicity in mice. 犬蔷薇果实水醇提取物对环磷酰胺诱导的小鼠睾丸毒性的保护作用。
IF 2.2 Q2 Medicine Pub Date : 2023-01-01 DOI: 10.22038/AJP.2022.20893
Rahmatollah Parandin, Mahnaz Ghowsi, Ahmad Dadbod

Objective: Cyclophosphamide (CP)-induced testicular toxicity has been reported in recipient patients. The current study was designed to evaluate protective effects of hydroalcoholic extract of Rosa canina L. fruit (HARF) against CP-induced testicular toxicity in BALB/c mice.

Materials and methods: Thirty-five mice were divided into five groups as follows: group I (control), group II (CP, received CP 100 mg/kg on days 1, 8, 15, and 22), group III (CP + HARF 250 mg/kg), group IV (CP + HARF 500 mg/kg), and group V (CP + HARF 750 mg/kg). In the groups III, IV, and V that received CP, the HARF was simultaneously administered via intraperitoneal injections for 28 consecutive days starting from day 1. On the 29th day, sperm parameters, stress oxidative biomarkers, and mRNA expression of nuclear factor erythroid-derived-2 (Nrf2) in testis tissue, as well as blood testosterone were evaluated.

Results: The CP exposure decreased sperm parameters, superoxide dismutase (SOD) activity, testosterone, and Nrf2 mRNA expression levels and increased the malondialdehyde (MDA). HARF at the dose of 500 mg/kg improved sperm count and viability and increased SOD and catalase activities, glutathione peroxidase (GPx) activity, testosterone level, and Nrf2 expression and reduced MDA. Also, HARF at the dose of 750 mg/kg improved sperm parameters and increased SOD, catalase, and GPx activities, total testosterone level, and Nrf2 expression, and reduced MDA in comparison with the CP group.

Conclusion: According to our findings, HARF at the doses of 500 and 750 mg/kg inhibited the ruinous effects of CP on the reproductive system in mice.

目的:报道了环磷酰胺(CP)在受体患者中引起的睾丸毒性。本研究旨在评估犬蔷薇果实水醇提取物(HARF)对CP诱导的BALB/c小鼠睾丸毒性的保护作用。材料和方法:35只小鼠分为五组:第一组(对照组)、第二组(CP,在第1、8、15和22天接受CP 100mg/kg)、第三组(CP+HARF 250mg/kg)、第四组(CP+ARF 500mg/kg)和第五组(CP+HARF 750mg/kg)。在接受CP的第III、IV和V组中,从第1天开始,通过腹膜内注射同时给予HARF连续28天。第29天,评估精子参数、应激氧化生物标志物、睾丸组织中核因子红细胞衍生2(Nrf2)的mRNA表达以及血液睾酮。结果:CP暴露降低了精子参数、超氧化物歧化酶(SOD)活性、睾酮和Nrf2mRNA表达水平,并增加了丙二醛(MDA)。500 mg/kg剂量的HARF改善了精子数量和活力,增加了SOD和过氧化氢酶活性、谷胱甘肽过氧化物酶(GPx)活性、睾酮水平和Nrf2表达,并降低了MDA。此外,与CP组相比,750mg/kg剂量的HARF改善了精子参数,增加了SOD、过氧化氢酶和GPx活性、总睾酮水平和Nrf2表达,并降低了MDA。结论:500和750mg/kg剂量的HARF抑制了CP对小鼠生殖系统的破坏性作用。
{"title":"Protective effects of hydroalcoholic extract of <i>Rosa canina</i> L. fruit on cyclophosphamide-induced testicular toxicity in mice.","authors":"Rahmatollah Parandin,&nbsp;Mahnaz Ghowsi,&nbsp;Ahmad Dadbod","doi":"10.22038/AJP.2022.20893","DOIUrl":"10.22038/AJP.2022.20893","url":null,"abstract":"<p><strong>Objective: </strong>Cyclophosphamide (CP)-induced testicular toxicity has been reported in recipient patients. The current study was designed to evaluate protective effects of hydroalcoholic extract of <i>Rosa canina</i> L. fruit (HARF) against CP-induced testicular toxicity in BALB/c mice.</p><p><strong>Materials and methods: </strong>Thirty-five mice were divided into five groups as follows: group I (control), group II (CP, received CP 100 mg/kg on days 1, 8, 15, and 22), group III (CP + HARF 250 mg/kg), group IV (CP + HARF 500 mg/kg), and group V (CP + HARF 750 mg/kg). In the groups III, IV, and V that received CP, the HARF was simultaneously administered via intraperitoneal injections for 28 consecutive days starting from day 1. On the 29<sup>th</sup> day, sperm parameters, stress oxidative biomarkers, and mRNA expression of <i>nuclear factor erythroid-derived-2 (Nrf2)</i> in testis tissue, as well as blood testosterone were evaluated.</p><p><strong>Results: </strong>The CP exposure decreased sperm parameters, superoxide dismutase (SOD) activity, testosterone, and <i>Nrf2</i> mRNA expression levels and increased the malondialdehyde (MDA). HARF at the dose of 500 mg/kg improved sperm count and viability and increased SOD and catalase activities, glutathione peroxidase (GPx) activity, testosterone level, and <i>Nrf2</i> expression and reduced MDA. Also, HARF at the dose of 750 mg/kg improved sperm parameters and increased SOD, catalase, and GPx activities, total testosterone level, and <i>Nrf2</i> expression, and reduced MDA in comparison with the CP group.</p><p><strong>Conclusion: </strong>According to our findings, HARF at the doses of 500 and 750 mg/kg inhibited the ruinous effects of CP on the reproductive system in mice.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10622338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The effect of hydroalcoholic extract of Cichorium intybus leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke. 菊苣叶水醇提取物对吸烟大鼠睾丸芳烃受体表达的影响。
IF 2.2 Q2 Medicine Pub Date : 2023-01-01 DOI: 10.22038/AJP.2022.21307
Maryam Hashemi, Mahnaz Azarnia, Zahra Hajebrahimi, Samad Nejad Ebrahimi

Objective: Cigarette smoke (CS) contains compounds such as reactive oxygen species (ROS). Oxidative stress caused by excessive ROS eventually leads to germ cell apoptosis and male infertility. The leaves of Cichorium intybus (chicory) are rich in natural antioxidants, but their protective effects on the adverse effects of CS on testicular tissue have not been studied.

Materials and methods: 24 Wistar rats were classified into four groups: control, extract: treatment with chicory extract (200 mg/kg body weight/day) for 13 weeks, smoke: exposed to CS for 13 weeks, and smoke + extract: exposed to CS and treated with the C. intybus extract. Histological and biochemical analyses and apoptosis assay were done, and Ahr, and Cyp1a1 expression was determined.

Results: Treatment with C. intybus compensated for the reduction of Sertoli cells, spermatogonia, spermatocytes, and spermatids caused by CS. Chicory extract reduced free radicals and improved antioxidant status. The lowest and highest percentage of apoptotic cells was observed in the extract and smoke groups, respectively, while simultaneous treatment with C. intybus extract led to a significant reduction of apoptotic cells. The mean Ahr levels in the control, extract, smoke and smoke + extract groups were 1.00±0.57, 1.93±0.25, 5.98±0.42, and 0.62±0.22, respectively (p˂0.05). The mean levels of Cyp1a1 expression in the control, extract, smoke and smoke + extract groups were 1.00±0.31, 2.28±0.65, 5.55±0.40, and 0.21±0.23 (p˂0.05).

Conclusion: The C. intybus extract probably affected Cyp1a1 expression by downregulation of Ahr. These led to a decrease in free radicals and apoptosis, and an improvement in antioxidant status.

目的:香烟烟雾(CS)中含有活性氧(ROS)等化合物。过量ROS引起的氧化应激最终导致生殖细胞凋亡和男性不育。菊苣的叶子富含天然抗氧化剂,但它们对CS对睾丸组织的不良影响的保护作用尚未得到研究。材料和方法:24只Wistar大鼠分为四组:对照组、提取物组:用菊苣提取物(200mg/kg体重/天)处理13周,烟雾组:暴露于CS 13周,以及烟雾+提取物组:暴露在CS并用C.intybus提取物处理。进行组织学和生化分析以及细胞凋亡测定,并测定Ahr和Cyp1a1的表达。结果:C.intybus治疗可补偿CS引起的支持细胞、精原细胞、精母细胞和精子细胞的减少。菊苣提取物减少自由基,改善抗氧化状态。在提取物组和烟雾组中分别观察到最低和最高百分比的凋亡细胞,而同时用C.intybus提取物处理导致凋亡细胞显著减少。对照组、提取物组、烟雾组和烟雾+提取物组的平均Ahr水平分别为1.00±0.57、1.93±0.25、5.98±0.42和0.62±0.22(p 0.05),和0.21±0.23(p 0.05)。结论:西洋参提取物可能通过下调Ahr而影响Cyp1a1的表达。这些导致自由基和细胞凋亡的减少,以及抗氧化状态的改善。
{"title":"The effect of hydroalcoholic extract of <i>Cichorium intybus</i> leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke.","authors":"Maryam Hashemi,&nbsp;Mahnaz Azarnia,&nbsp;Zahra Hajebrahimi,&nbsp;Samad Nejad Ebrahimi","doi":"10.22038/AJP.2022.21307","DOIUrl":"10.22038/AJP.2022.21307","url":null,"abstract":"<p><strong>Objective: </strong>Cigarette smoke (CS) contains compounds such as reactive oxygen species (ROS). Oxidative stress caused by excessive ROS eventually leads to germ cell apoptosis and male infertility. The leaves of <i>Cichorium intybus</i> (chicory) are rich in natural antioxidants, but their protective effects on the adverse effects of CS on testicular tissue have not been studied.</p><p><strong>Materials and methods: </strong>24 Wistar rats were classified into four groups: control, extract: treatment with chicory extract (200 mg/kg body weight/day) for 13 weeks, smoke: exposed to CS for 13 weeks, and smoke + extract: exposed to CS and treated with the C. <i>intybus</i> extract. Histological and biochemical analyses and apoptosis assay were done, and <i>Ahr,</i> and <i>Cyp1a1</i> expression was determined.</p><p><strong>Results: </strong>Treatment with <i>C. intybus</i> compensated for the reduction of Sertoli cells, spermatogonia, spermatocytes, and spermatids caused by CS. Chicory extract reduced free radicals and improved antioxidant status. The lowest and highest percentage of apoptotic cells was observed in the extract and smoke groups, respectively, while simultaneous treatment with <i>C. intybus</i> extract led to a significant reduction of apoptotic cells. The mean <i>Ahr</i> levels in the control, extract, smoke and smoke + extract groups were 1.00±0.57, 1.93±0.25, 5.98±0.42, and 0.62±0.22, respectively (p˂0.05). The mean levels of <i>Cyp1a1</i> expression in the control, extract, smoke and smoke + extract groups were 1.00±0.31, 2.28±0.65, 5.55±0.40, and 0.21±0.23 (p˂0.05).</p><p><strong>Conclusion: </strong>The <i>C. intybus</i> extract probably affected <i>Cyp1a1</i> expression by downregulation of <i>Ahr.</i> These led to a decrease in free radicals and apoptosis, and an improvement in antioxidant status.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Avicenna Journal of Phytomedicine
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