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Characterization of the quality of the steamed yoghurts enriched by dates flesh and date powder variety H’loua 红枣果肉和红枣粉加味蒸酸奶的品质表征
Pub Date : 2018-05-15 DOI: 10.7904/2068-4738-IX(17)-31
A. Hariri, N. Ouis, D. Bouhadi, Zouaoui Benatouche
The purpose of the present work was to investigate the effect of including date flesh and date powder in the steamed yoghurt processing on some quality during four weeks of storage. In order to obtain the powders, date variety of H’loua was washed, cut into small particles, and then dried at 90 °C during 2 h. Seven steamed yoghurts were manufactured: control prepared by 80 g/L sucrose, yoghurts manufactured by replacement of some quantity of sucrose by 10, 20 and 25 g/L of the date powder and yoghurts prepared by replacement of some quantity of sucrose by 60, 70 and equal quantity 80 g/L of date flesh. All samples were analyzed for some physicochemical, biochemical, microbiological and sensory characteristics at 1, 7, 15, 21 and 29 days of storage. The total or partial replacement of the sucrose by date flesh and date powder improves the nutritional quality of the yoghurts such as proteins, fat, ash, and dry mater, but the level of the total sugars was decreased. Results of microbiological analyze showed complete absence of the total and fecal coliforms, Staphylococcus aureus, faecal streptococci and Salmonella in all yoghurts prepared and throughout the storage period. Replacement of the sucrose by all types of date improves the sensory characteristics of the yoghurts included taste and texture. Keyword: Date flesh, date powder, H’loua, Quality, Sucrose, Yoghurt.
研究了在酸奶蒸制过程中加入枣果肉和枣粉对酸奶在4周贮藏过程中某些品质的影响。为获得粉末状,将H 'loua枣品种洗净,切成小颗粒,90℃下烘干2 H,制备出7种蒸制酸奶:以80 g/L蔗糖为对照,以10、20、25 g/L枣粉代替一定量蔗糖制成酸奶,以60、70、等量80 g/L枣果肉代替一定量蔗糖制成酸奶。在贮藏1、7、15、21和29 d时,对所有样品进行理化、生化、微生物学和感官特性分析。用枣果肉和枣粉替代全部或部分蔗糖,提高了酸奶的蛋白质、脂肪、灰分和干物质等营养品质,但降低了总糖含量。微生物学分析结果显示,在所有酸奶制备和整个贮存期间,总大肠菌群和粪便大肠菌群、金黄色葡萄球菌、粪便链球菌和沙门氏菌完全不存在。用各种类型的枣代替蔗糖可以改善酸奶的感官特征,包括口感和质地。关键词:枣果肉,枣粉,哈洛瓦,品质,蔗糖,酸奶
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引用次数: 15
Antixenosis Resistance in Sugar Beet Varieties to Long Snout Weevil Lixus incanescens Boh. (Col.: Curculionidae) 甜菜品种对长鼻象鼻虫的抗虫性研究(Col。:象甲科)
Pub Date : 2018-05-15 DOI: 10.7904/2068-4738-ix(17)-24
F. H. Moghadam, J. Khalghani, S. Moharramipour, B. Gharali, Mehrzad Mostashari Mohasses
In plants, antixenosis resistance could reduce injury and yield loss; they can produce selection pressures on insect herbivores that lead to pest resistance. Traditionally, sugar beet long snout weevil, Lixus incanescens Boh. (Col.: Curculionidae), is one of the serious pests causing sugar beet losses. In the present study, antixenosis resistance was evaluated in 11 varieties of sugar beet including Pars, Torbat, Ekbatan, SBSI019, Rizofort, Puma, Dorothea, Brigita, Pecora, Nagano and Jolgehin a field trial from 2013 and 2014 years in Qazvin province, Iran. A randomized complete block design with their replications was used. The results showed that in both years, in the first 24 h experiment after release the lowest and highest densities of pest (No. adult/plant) were seen on Pars (2.99) and Dorothea (2.83) varieties, respectively, as opposed the lowest antixenosis resistance were found on Brigita (8.66) and Nagano (8.83) cultivar, whereas on other cultivars showed intermediate values. In Pars, Brigita and Nagano varieties the number of adult weevils (adult/plant) increased rapidly during the first 24 h, but after which there was a decline to the minimum on about the fifth day. The results of data analysis to investigate the mechanism of free choice antixenosis in the years 2013 and 2014 showed that there are no significant differences between experimental blocks and among cultivars tested. As a result, our findings showed that above mention variety can be used in integrated management plans of sugar beet long snout weevil, and with regionally adapted varieties and excellent seed quality we strive for excellent varietal performance in all growing regions of the Iran. Keyword: Antixenosis, Lixus incanescens, Resistance, Sugar beet, Weevil.
在植物中,抗蛇毒可以减少伤害和产量损失;它们可以对食草昆虫产生选择压力,从而导致害虫抗性。传统上,甜菜长鼻象鼻虫,白杨。甜菜螟是造成甜菜严重损失的害虫之一。本研究通过2013年和2014年在伊朗Qazvin省进行的田间试验,对Pars、Torbat、Ekbatan、SBSI019、Rizofort、Puma、Dorothea、Brigita、Pecora、Nagano和Jolgehin等11个甜菜品种的抗xenosis抗性进行了评价。采用随机完全区组设计,并进行重复。结果表明,两年放生后的前24 h试验中,害虫的最低密度和最高密度分别为:Pars品种(2.99)和Dorothea品种(2.83)的成虫/株的抗性最低,Brigita品种(8.66)和长野品种(8.83)的抗性最低,其余品种均为中等水平。帕尔斯、布丽吉塔和长野品种成虫数(成虫/株)在前24 h迅速增加,之后在第5 d左右降至最低。对2013年和2014年自由选择抗异种中毒机理的数据分析结果表明,不同试验区和品种间的抗异种中毒机制差异不显著。因此,我们的研究结果表明,上述品种可以用于甜菜长鼻象鼻虫的综合管理计划,并且具有区域适应性品种和优良的种子品质,我们争取在伊朗所有产区的优良品种表现。关键词:抗蛇毒,白柳,抗性,甜菜,象鼻虫。
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引用次数: 2
Toxicity and repellency of three Algerian medicinal plants against pests of stored product: Ryzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) 阿尔及利亚3种药用植物对储存品害虫的毒性及驱避性研究
Pub Date : 2018-05-15 DOI: 10.7904/2068-4738-IX(17)-50
K. Righi, F. Righi, Ahlem Boubkeur, K. Boungab, A. Elouissi, Ali Cherif Djendara
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引用次数: 11
Constructional and Functional Evaluation of Two New Plant Expression Vectors—pBI121gus-6 and pBI1215+1 两个新的植物表达载体pbi121gus -6和pBI1215+1的构建及功能评价
Pub Date : 2018-05-15 DOI: 10.7904/2068-4738-IX(17)-60
Reza Mohammadhassan, K. Esfahani, B. Kashefi
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引用次数: 5
Investigation of the induced antibiosis resistance by zinc element in different cultivars of sugar beet to long snout weevil, Lixus incanescens (Col: Curculionidae) 不同品种甜菜锌元素诱导对长鼻象抗性的研究
Pub Date : 2018-05-15 DOI: 10.7904/2068-4738-IX(17)-5
F. H. Moghadam, J. Khalghani, S. Moharramipour, B. Gharali, Mehrzad Mostashari Mohasses
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引用次数: 13
Effects of electromagnetic field radiation on biochemical parameters in swiss albino mice 电磁场辐射对瑞士白化病小鼠生化指标的影响
Pub Date : 2017-10-26 DOI: 10.7904/2068-4738-VIII(16)-48
S. Peighambarzadeh, M. Tavana
7784 Abstract . This study investigated the effects of electromagnetic field radiated from mobile phones with frequency between 500 to 900 MHz on some biochemical parameters in 60 swiss albino mice which divided in three groups (one control and two tests). Test groups were exposed to electromagnetic wave twice a day for 20 and 40 days. Blood samples were prepared from heart and Total Protein, Albumin, Blood Urea Nitrogen (BUN), Creatinine Phosphokinase (CPK), Cholesterol, Creatinine, Glucose, Alanin Amino Transferase (ALT), Aspartate Amino Transferase (AST), Alkaline Phosphatase (ALP), Amylase, Lipase, Thyroid Stimulating Hormone (TSH), T 3 and Tyroxin (T 4) were measured and statistically analysied. According to the results, long time electromagnetic field radiation caused a significant decrease in serum creatinine, glucose, total protein, albumine, cholesterol, T 4 and body weight. The amount of Serum BUN, CPK, ALP, ALT, AST, TSH and T 3 significantly increased. Electromagnetic radiation has influence on biochemical parameters in mice and may effected on
7784摘要。本研究研究了频率在500 ~ 900 MHz之间的手机电磁场对60只瑞士白化病小鼠某些生化参数的影响,这些小鼠被分为三组(一对照组和两组试验)。试验组每天两次暴露于电磁波中,持续20天和40天。取心脏血样,测定总蛋白、白蛋白、血尿素氮(BUN)、肌酐磷酸激酶(CPK)、胆固醇、肌酐、葡萄糖、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、淀粉酶、脂肪酶、促甲状腺激素(TSH)、t3、酪氨酸(t4)含量,并进行统计学分析。结果表明,长时间电磁场辐射导致大鼠血清肌酐、葡萄糖、总蛋白、白蛋白、胆固醇、t4和体重显著下降。血清BUN、CPK、ALP、ALT、AST、TSH、t3含量显著升高。电磁辐射对小鼠的生化指标有影响,并可能对人体产生影响
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引用次数: 3
Assessment of immunogenicity of alginate microparticle containing Brucella melitensis 16M oligo polysaccharide tetanus toxoid conjugate in mouse 含布氏菌16M寡糖海藻酸盐微粒子对小鼠免疫原性的评价
Pub Date : 2017-10-26 DOI: 10.7904/2068-4738-VIII(16)-83
Parivash Ghaderinia, R. Shapouri
Brucellosis is an infectious disease caused by Brucella bacteria, affecting animals and humans. It is found as an endemic disease in many parts of the world, especially in the Middle East, Central Asian, and South American countries. The objective of this study was to prepare alginate microparticle containing conjugate of Brucella melitensis 16 M oligopolysaccharide (OPS) with tetanus toxoid, and to assess its immunogenicity in mouse, in order to find a new method of confronting brucellosis. In this study, we extracted OPS from lipopolysaccharide (LPS) and conjugated it with tetanus toxoid by amidation method. Then, the obtained conjugate was condensed by gel filtration, followed by emulcification method in iso–octane organic phase to prepare alginate microparticles containing the conjugate. In our study, sera titr of antibodies IgM, IgA, IgG2a, IgG1, IgG2b, IgG3, and total IgG generated against oligopolysaccharide was performed by ELISA test. The final results, considering the obtained immunogenicity and the increased titer of antibodies IgG1 (19 times), IgG3 (19 times), IgG (15 times), and IgM (9 times) after injection of produced microparticle in mouse indicates appropriateness of the produced microparticle as a candidate vaccine. Thus, it could be concluded that alginate microparticles containing conjugates of B.melitensis 16 M oligopolysaccharide with tetanus toxoid can be proposed as a candidate anti– brucellosis vaccine. Keyword: Brucella melitensis, Microparticle, Tetanus, Alginate, Vaccine.
布鲁氏菌病是一种由布鲁氏菌引起的传染病,影响动物和人类。在世界许多地方,特别是在中东、中亚和南美国家,发现它是一种地方病。本研究的目的是制备含有美氏布鲁氏菌16m寡聚糖(OPS)与破伤风类毒素偶联物的藻酸盐微球,并对其进行小鼠免疫原性评价,以期找到一种对抗布鲁氏菌病的新方法。本研究从脂多糖(LPS)中提取OPS,并采用酰胺化法与破伤风类毒素偶联。然后,将得到的共轭物通过凝胶过滤浓缩,然后在异辛烷有机相中进行乳化法制备含有该共轭物的海藻酸盐微颗粒。在我们的研究中,通过ELISA检测血清中抗寡多糖的抗体IgM、IgA、IgG2a、IgG1、IgG2b、IgG3和总IgG的滴度。最终的结果,考虑所得的免疫原性和小鼠注射后IgG1(19倍)、IgG3(19倍)、IgG(15倍)和IgM(9倍)抗体滴度的提高,表明所制微粒作为候选疫苗是合适的。由此可见,含有B.melitensis 16m寡聚糖与破伤风类毒素结合物的海藻酸盐微颗粒可作为抗布鲁氏菌病的候选疫苗。关键词:布鲁氏菌,微粒,破伤风,海藻酸盐,疫苗
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引用次数: 7
Antibacterial effects of aqueous and organic quince leaf extracts on gram–positive and gram–negative bacteria 木瓜叶水提液和有机提液对革兰氏阳性菌和革兰氏阴性菌的抑菌作用
Pub Date : 2017-10-26 DOI: 10.7904/2068-4738-VIII(16)-54
S. N. Semnani, N. Hajizadeh, H. Alizadeh
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引用次数: 14
Isolation and cloning of flanking regions (ndhB–rps7) of plastidial vector from lettuce 莴苣质体侧翼区ndhB-rps7的分离与克隆
Pub Date : 2017-10-26 DOI: 10.7904/2068-4738-VIII(16)-5
Mahdi Gholipour, B. Kohnehrouz
33356003 Abstract . One of the advantages of chloroplast engineering is the entrance of targeted gene in to a specific region of the chloroplast genome. Due to advantage of this method than nuclear genetic engineering, it is one of the safest ways for the production of recombinant biopharmaceuticals, vaccines etc ., in the plants. This is done due to the presence of homologous sequences from chloroplast genome of host plant on both sides of the gene cassette and the occurrence of homologous recombination. In this research, cloning of ndhB–rps7 fragment was considered from lettuce chloroplast genome as flanking regions. The designing of specific primers for amplification of these regions was done by Primer 3 and Oligo Analyzer softwares. After total genomic DNA extraction, PCR amplification of fragment was done and cloning of this fragment was carried out by T/A procedure in to p TG19–T vector. The recombinant plasmids were sent to Genefanavaran co for sequencing and determining the restriction sites in the cloned fragment. The validation of ndhB–rps7 fragment was confirmed with the lettuce 1380 bp through the ‘BLAST’ software. This work is the first step in the production of biomaterials in
33356003摘要叶绿体工程的优点之一是目标基因进入叶绿体基因组的特定区域。由于该方法比核基因工程具有优势,是植物内生产重组生物制药、疫苗等最安全的方法之一。这是由于基因盒两侧存在寄主植物叶绿体基因组的同源序列,并发生同源重组。本研究考虑从莴苣叶绿体基因组中克隆ndhB-rps7片段作为侧翼区。利用Primer 3和Oligo Analyzer软件设计特异性引物扩增这些区域。提取总基因组DNA后,对该片段进行PCR扩增,并在TG19-T载体上进行T/A程序克隆。将重组质粒送到Genefanavaran co进行测序并确定克隆片段的限制性内切位点。ndhB-rps7片段通过BLAST软件以莴苣1380 bp为样本进行验证。这项工作是生物材料生产的第一步
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引用次数: 1
Assessment of HIV infection in cells of infected individuals 感染个体细胞中HIV感染的评估
Pub Date : 2017-10-26 DOI: 10.7904/2068-4738-VIII(16)-24
Jafar Aghajani, S. Mirtajani, S. Kojuri, Reihane Zaheire, S. Ayoubi
HIV is one of the most prevalent opportunistic pathogens in cells of infected individuals and can cause diseases such as: encephalitis, pneumonia and chorioretinitis. The aim of this study is to investigate HIV infection in cells infected persons. This study was performed on 50 blood samples from individuals infected with HIV. Studied patients in the two groups were consumer and nonconsumer of antiretroviral drugs (mention the name of the Antiviral drug). After separation of plasma from the blood samples to detect HIV in cell culture and PCR were examined. Of the 50 patients, 56 % (n = 28) were male and 44 percent (n = 22) were women. DNA antibodies in 16 % (8 cases) were identified in the population, the prevalence of HIV infection in men 21.4 % (6 cases) and in women 1.9% (2 cases). The high prevalence of HIV infection in patients treated with CMV (full form of CMV), the treatment of HIV infection should be considered. Keyword: HIV infection, cells infection, analyses, characteristics.
艾滋病毒是感染个体细胞中最普遍的机会性病原体之一,可引起脑炎、肺炎和绒毛膜视网膜炎等疾病。本研究的目的是研究HIV在细胞感染者中的感染情况。这项研究是在50个艾滋病毒感染者的血液样本上进行的。研究的两组患者是抗逆转录病毒药物的消费者和非消费者(提及抗病毒药物的名称)。分离血浆后从血样中检测HIV,进行细胞培养和PCR检测。在50例患者中,56% (n = 28)为男性,44% (n = 22)为女性。人群中发现DNA抗体率为16%(8例),男性为21.4%(6例),女性为1.9%(2例)。在接受巨细胞病毒(CMV)治疗的患者中HIV感染率较高,应考虑对HIV感染的治疗。关键词:HIV感染,细胞感染,分析,特征。
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引用次数: 3
期刊
Banat's Journal of Biotechnology
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