Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-93
N. Ouis, A. Hariri
90 Abstract . This study was carried out to determine the phytochemical profile and antioxidant activity of the carob. The results of preliminary phytochemical assessed showed that the alkaloid bases and salts, flavones aglycones, reducing compounds, fatty acids, polyterpenes, catechin tannins, volatile oils, cardiac glycosides, flavonoids, sterol glycosides and terpenes, amino acids and saponins are classes of chemical groups present in the pods of Ceratonia siliqua . The pods are characterized by 87 ± 1.0 % dry matter, 55 ± 0.2 % total sugars, 4 ± 0.1 % proteins, 7 ± 0.4 % lipids, 4 ± 0.3 ash. We have found that the pod has a 10.56 ± 0.2 Na, 0.75 ± 0.1 Zn, 0.55 ± 0.3 Cu, 41.7 ± 0.2 Mg, 210 ± 0.3 Ca and 1150 ± 0.2 mg/100g K. Pods of carob present 71.6 ± 2.10 mg/100g of flavonoids, 56.51 ± 1.02 mg/100g of alkaloids, 41.25 ± 1.36 mg/100g of saponins and 1.18 ± 0.2 mg/100g of tannins. Flavonoids fractions were extracted using organic solvents with different polarity. The ethyl acetate extract has the highest content of polyphenols and flavonoids 259.4 ± 4.2, 71.34 ± 1.08 mg EGA/g E, followed by the n–butanol extract 62.19 ± 0.13, 53.01 ± 0.11, then the crude extract 15.5 ± 0.21, 12.9 ± 0.17 and finally aqueous extract 13.39 ± 0.27, 11.13 ± 0.62. The flavonoids fractions extracted by ethyl acetate and n–butanol showed the higher antioxidant capacity determined by three methods: free radical scavenging activity, reducing power and liver lipid peroxidation compared to the crude exact and aqueous
{"title":"Phytochemical analysis and antioxidant activity of the flavonoids extracts from pods of Ceratonia siliqua L.","authors":"N. Ouis, A. Hariri","doi":"10.7904/2068-4738-VIII(16)-93","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-93","url":null,"abstract":"90 Abstract . This study was carried out to determine the phytochemical profile and antioxidant activity of the carob. The results of preliminary phytochemical assessed showed that the alkaloid bases and salts, flavones aglycones, reducing compounds, fatty acids, polyterpenes, catechin tannins, volatile oils, cardiac glycosides, flavonoids, sterol glycosides and terpenes, amino acids and saponins are classes of chemical groups present in the pods of Ceratonia siliqua . The pods are characterized by 87 ± 1.0 % dry matter, 55 ± 0.2 % total sugars, 4 ± 0.1 % proteins, 7 ± 0.4 % lipids, 4 ± 0.3 ash. We have found that the pod has a 10.56 ± 0.2 Na, 0.75 ± 0.1 Zn, 0.55 ± 0.3 Cu, 41.7 ± 0.2 Mg, 210 ± 0.3 Ca and 1150 ± 0.2 mg/100g K. Pods of carob present 71.6 ± 2.10 mg/100g of flavonoids, 56.51 ± 1.02 mg/100g of alkaloids, 41.25 ± 1.36 mg/100g of saponins and 1.18 ± 0.2 mg/100g of tannins. Flavonoids fractions were extracted using organic solvents with different polarity. The ethyl acetate extract has the highest content of polyphenols and flavonoids 259.4 ± 4.2, 71.34 ± 1.08 mg EGA/g E, followed by the n–butanol extract 62.19 ± 0.13, 53.01 ± 0.11, then the crude extract 15.5 ± 0.21, 12.9 ± 0.17 and finally aqueous extract 13.39 ± 0.27, 11.13 ± 0.62. The flavonoids fractions extracted by ethyl acetate and n–butanol showed the higher antioxidant capacity determined by three methods: free radical scavenging activity, reducing power and liver lipid peroxidation compared to the crude exact and aqueous","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"29 1","pages":"93-104"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83460476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-75
A. Hariri, N. Ouis, D. Bouhadi, K. O. Yerou
This study was carried out to the valuing of the date variety H’mira (very rich’s in sugars and in nourishing elements) and the cheese whey for production of novel syrups. Four samples syrups were manufactured: control represent date syrup obtained after hot extraction at a concentration 20 %, syrups (A) represent control with 10 % of cheese whey, syrup (B) with 20 % and syrup (C) with 30 % of cheese whey. These syrups were stored during 3 weeks at 4 °C. The biochemical, microbiological and sensory evaluations for all syrups were evaluated every week of storage. The biochemical characterization of syrups showed that it’s richness in total sugars, proteins contents, ash and dry mater. The syrup of date enriched with 10 % of liquid acid cheese whey present a good biochemical quality (higher level of sugars, proteins and ash) and important sensorial properties (color, odor and flavor). This syrup can be used for beverage or as medium to fermentation for production a new products. Keyword: Date, Syrup, H’mira, Cheese whey, Quality, Storage.
{"title":"Evaluation of the quality of the date syrups enriched by cheese whey during the period of storage","authors":"A. Hariri, N. Ouis, D. Bouhadi, K. O. Yerou","doi":"10.7904/2068-4738-VIII(16)-75","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-75","url":null,"abstract":"This study was carried out to the valuing of the date variety H’mira (very rich’s in sugars and in nourishing elements) and the cheese whey for production of novel syrups. Four samples syrups were manufactured: control represent date syrup obtained after hot extraction at a concentration 20 %, syrups (A) represent control with 10 % of cheese whey, syrup (B) with 20 % and syrup (C) with 30 % of cheese whey. These syrups were stored during 3 weeks at 4 °C. The biochemical, microbiological and sensory evaluations for all syrups were evaluated every week of storage. The biochemical characterization of syrups showed that it’s richness in total sugars, proteins contents, ash and dry mater. The syrup of date enriched with 10 % of liquid acid cheese whey present a good biochemical quality (higher level of sugars, proteins and ash) and important sensorial properties (color, odor and flavor). This syrup can be used for beverage or as medium to fermentation for production a new products. Keyword: Date, Syrup, H’mira, Cheese whey, Quality, Storage.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"16 1","pages":"75-82"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87773018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-127
L. Derakhshan, S. Emami
Medicinal plants have been used for treatment of various diseases. The potent scolicidal activity of medicinal plants has been also known. Thus, the current study was conducted to investigate the protoscolicidal effects of Bunium Persicum against hydatid cyst protoscoleces. Protoscoleces were collected from sheep's kidney having hydatid cysts. Five concentrations of B. Persicum essential oil (1, 3, 5, 10 and 15 mg/mL) were investigated for 10, 20, 30 and 60 min. Viability of protoscoleces was confirmed using eosin exclusive test. Regardless of the time, the rate of dead protoscoleces was increased with increasing the essential oil concentrations. The most rates of dead protoscoleces (100 %) were observed in 15 mg/mL from 10 to 60 minutes. On the basis findings this in vitro study, it can be advised B. Persicum essential oil as an effective natural scolicidal agent. Keyword: Bunium Persicum, Hydatid cyst, Protoscoleces, Scolicidal activity.
{"title":"Protoscolicidal Effects of Bunium Persicum (Boiss) Against Hydatid Cyst Protoscoleces","authors":"L. Derakhshan, S. Emami","doi":"10.7904/2068-4738-VIII(16)-127","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-127","url":null,"abstract":"Medicinal plants have been used for treatment of various diseases. The potent scolicidal activity of medicinal plants has been also known. Thus, the current study was conducted to investigate the protoscolicidal effects of Bunium Persicum against hydatid cyst protoscoleces. Protoscoleces were collected from sheep's kidney having hydatid cysts. Five concentrations of B. Persicum essential oil (1, 3, 5, 10 and 15 mg/mL) were investigated for 10, 20, 30 and 60 min. Viability of protoscoleces was confirmed using eosin exclusive test. Regardless of the time, the rate of dead protoscoleces was increased with increasing the essential oil concentrations. The most rates of dead protoscoleces (100 %) were observed in 15 mg/mL from 10 to 60 minutes. On the basis findings this in vitro study, it can be advised B. Persicum essential oil as an effective natural scolicidal agent. Keyword: Bunium Persicum, Hydatid cyst, Protoscoleces, Scolicidal activity.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"29 1","pages":"127-132"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81577192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-105
F. Barazesh, H. Oloumi, F. Nasibi, K. M. Kalantari
Several physiological problems such as abscission of inflorescence buds and fruit, incidence of blank, non–split and deformed nuts leads to diminish yield of pistachio trees. This research designed study the effects of epibrassinolid and spermine application on fruit disorders and abscission on Pistacia vera “Ahmad–Aghai” cultivar. Shoots, of pistachio trees sprayed with Epi–epibrassinolid and spermine, with three replicates. The hormonal treatments consisted of, 0.5 and 1 ppm Epi–Br and 100 and 200 ppm Spm and their combination. Some yield traits including inflorescence and fruit abscission were studied. Fruit abscission diminished significantly in all treatments in comparison with untreated hormonal trees. Epibrassinolid and spermine treatments decreased fruit blankness percentage and ethylene emission in compared with control. Simlutanouse application of epibrassinolid and spermine effectively raised split percentage in compared to each hormonal treatment alone. Chlorophyll content and carbohydrate content significantly increased by spermine 100 and 200 ppm, epibrassinolid 0.5 ppm and combination of spermine and epibrassinolid 0.5 in compared with the control. According to the results, it seems that spermine, epibrassinolid and combination of these hormones could significantly reduce fruit abscission and improve some of the physiological parameters in pistachio trees, probably through the antagonistic effects on ethylene production. Keyword: Ethylene emission, Fruit and bud abscission, fruit disorder, pistachio yield.
{"title":"Effect of Spermine, Epibrassinolid and their interaction on inflorescence buds and fruits abscission of pistachio tree (Pistacia vera L.), “Ahmad–Aghai” cultivar","authors":"F. Barazesh, H. Oloumi, F. Nasibi, K. M. Kalantari","doi":"10.7904/2068-4738-VIII(16)-105","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-105","url":null,"abstract":"Several physiological problems such as abscission of inflorescence buds and fruit, incidence of blank, non–split and deformed nuts leads to diminish yield of pistachio trees. This research designed study the effects of epibrassinolid and spermine application on fruit disorders and abscission on Pistacia vera “Ahmad–Aghai” cultivar. Shoots, of pistachio trees sprayed with Epi–epibrassinolid and spermine, with three replicates. The hormonal treatments consisted of, 0.5 and 1 ppm Epi–Br and 100 and 200 ppm Spm and their combination. Some yield traits including inflorescence and fruit abscission were studied. Fruit abscission diminished significantly in all treatments in comparison with untreated hormonal trees. Epibrassinolid and spermine treatments decreased fruit blankness percentage and ethylene emission in compared with control. Simlutanouse application of epibrassinolid and spermine effectively raised split percentage in compared to each hormonal treatment alone. Chlorophyll content and carbohydrate content significantly increased by spermine 100 and 200 ppm, epibrassinolid 0.5 ppm and combination of spermine and epibrassinolid 0.5 in compared with the control. According to the results, it seems that spermine, epibrassinolid and combination of these hormones could significantly reduce fruit abscission and improve some of the physiological parameters in pistachio trees, probably through the antagonistic effects on ethylene production. Keyword: Ethylene emission, Fruit and bud abscission, fruit disorder, pistachio yield.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"20 1","pages":"105-115"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81932551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-18
S. Rodino, M. Buțu, C. Gaidău, Manuela Calin, A. Buțu
The development of in silico methods with application in scientific research is in full progress. They have the advantage of being modest consumers of material resources and time and, also, of being easy to be used by researchers from various field of specialization. The research work described in this paper has pursued building an ontological model for the evaluation of the impact of nanoparticle on the human cell. This model can be used to determine the inflammatory and cytotoxic effects of nanomaterials on human cells by complements and refining of the data achieved by well–known laboratory techniques. Keyword: ontology, modelling, citotoxicity, nanoparticles.
{"title":"Ontological model for the evaluation of the impact of nanoparticles on the human cell morphology","authors":"S. Rodino, M. Buțu, C. Gaidău, Manuela Calin, A. Buțu","doi":"10.7904/2068-4738-VIII(16)-18","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-18","url":null,"abstract":"The development of in silico methods with application in scientific research is in full progress. They have the advantage of being modest consumers of material resources and time and, also, of being easy to be used by researchers from various field of specialization. The research work described in this paper has pursued building an ontological model for the evaluation of the impact of nanoparticle on the human cell. This model can be used to determine the inflammatory and cytotoxic effects of nanomaterials on human cells by complements and refining of the data achieved by well–known laboratory techniques. Keyword: ontology, modelling, citotoxicity, nanoparticles.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"29 1","pages":"18-23"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85442933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-12
F.P.D. Satimehin, L. O. Tiamiyu, R. G. Okayi
Rubber leaves are a large by-product of rubber plantations which could serve as an important source of unconventional feed of agro by-product origin. However, it contains antinutritional factors which limits it utilization on a large scale as feed for animal nutrition. This study seeks to improve the nutritional value of rubber leaves meal through hydrothermal processing. Fresh leaves of rubber were collected from Rubber Research Institute of Nigeria in Benin City, and divided into five batches; four of these batches were subjected to varying periods of hydrothermal processing namely, 5, 10, 15 and 20 minutes, while one batch was kept as control without processing. Result reveals significant reduction in phytic acid, Hydrocyanic acid and Tannin as the processing time progressed. However, crude protein increased till the 10 min and significantly reduced thereafter and contrary to this, fat and ash significantly reduced as the time increased. Nutritional trials with various animals are recommended to evaluate feed utilization as a result of this processing method. Keyword: Unconventional feed stuff, Hydrogen Cyanide, Crude protein, Antinutritional factor.
{"title":"Proximate and phytochemical changes in hydrothermally processed rubber (Hevea brasiliensis) leaf meal","authors":"F.P.D. Satimehin, L. O. Tiamiyu, R. G. Okayi","doi":"10.7904/2068-4738-VIII(16)-12","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-12","url":null,"abstract":"Rubber leaves are a large by-product of rubber plantations which could serve as an important source of unconventional feed of agro by-product origin. However, it contains antinutritional factors which limits it utilization on a large scale as feed for animal nutrition. This study seeks to improve the nutritional value of rubber leaves meal through hydrothermal processing. Fresh leaves of rubber were collected from Rubber Research Institute of Nigeria in Benin City, and divided into five batches; four of these batches were subjected to varying periods of hydrothermal processing namely, 5, 10, 15 and 20 minutes, while one batch was kept as control without processing. Result reveals significant reduction in phytic acid, Hydrocyanic acid and Tannin as the processing time progressed. However, crude protein increased till the 10 min and significantly reduced thereafter and contrary to this, fat and ash significantly reduced as the time increased. Nutritional trials with various animals are recommended to evaluate feed utilization as a result of this processing method. Keyword: Unconventional feed stuff, Hydrogen Cyanide, Crude protein, Antinutritional factor.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"10 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84929167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-148
A. Saidi, Yazdan Eghbalnegad, Z. Hajibarat
This study was undertaken to evaluate genetic diversity in a germplasm consisting of rose varieties. Genetic distances were estimated using three different molecular marker techniques including: start codon targeted (SCoT), conserved DNA–Derived Polymorphism (CDDP) and directly amplified minisatellite DNA (DAMD). According to the results, the average polymorphism information content was 0.37, 0.36, and 0.36 for SCoT, CDDP and DAMD markers, respectively indicating that the studied marker types were equal in terms of assessing diversity. Cluster analysis using SCoT and CDDP divided the varieties to four distinct clusters whereas DAMD markers data, grouped the varieties into three clusters. There was a positive significant correlation (r=0.92, p<0.01) between similarity matrix obtained by SCoT and CDDP. Results suggested that the efficiency of SCOT, CDDP and DAMD markers had a relatively same efficiency in fingerprinting of varieties. This is the first time that the efficiency of the three molecular markers have been compared with each other in a set of rose samples. The results showed that the studied markers had an appropriate polymorphism and thus were suitable for the study of genetic diversity in rose. Keyword: Fingerprinting, PIC, molecular marker, genetic distance, correlation.
{"title":"Study of genetic diversity in local rose varieties (Rosa spp.) using molecular markers","authors":"A. Saidi, Yazdan Eghbalnegad, Z. Hajibarat","doi":"10.7904/2068-4738-VIII(16)-148","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-148","url":null,"abstract":"This study was undertaken to evaluate genetic diversity in a germplasm consisting of rose varieties. Genetic distances were estimated using three different molecular marker techniques including: start codon targeted (SCoT), conserved DNA–Derived Polymorphism (CDDP) and directly amplified minisatellite DNA (DAMD). According to the results, the average polymorphism information content was 0.37, 0.36, and 0.36 for SCoT, CDDP and DAMD markers, respectively indicating that the studied marker types were equal in terms of assessing diversity. Cluster analysis using SCoT and CDDP divided the varieties to four distinct clusters whereas DAMD markers data, grouped the varieties into three clusters. There was a positive significant correlation (r=0.92, p<0.01) between similarity matrix obtained by SCoT and CDDP. Results suggested that the efficiency of SCOT, CDDP and DAMD markers had a relatively same efficiency in fingerprinting of varieties. This is the first time that the efficiency of the three molecular markers have been compared with each other in a set of rose samples. The results showed that the studied markers had an appropriate polymorphism and thus were suitable for the study of genetic diversity in rose. Keyword: Fingerprinting, PIC, molecular marker, genetic distance, correlation.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"25 1 1","pages":"148-157"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90762735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-69
A. Coroian, V. Mireșan, D. Cocan, C. Răducu, A. Longodor, A. Pop, Gratian Feher, Luisa Andronie, Z. Marchiș
Heavy metals are important pollutants in the environment and can cause problems for the organisms, and the bioaccumulation of heavy metals in the food chain can have adverse effects on human health. Regulations on the legally accepted amounts of heavy metals in food and in the body are very precise. The purpose of this paper was to evaluate the level of heavy metals in cow milk from different localities in the Baia Mare area, considered a very polluted area. The physical–chemical parameters and heavy metals in milk were determined. The determination of heavy metals in milk was performed by inductively coupled plasma mass spectrometry or ICP– MS used to identify and quantify the Pb, Cd, Cu, and Zn elements. The highest concentrations of Pb were observed in samples collected in Firiza locality with an average value of (43.22±0.62) (μg/L) and the lowest concentrations are for the samples analyzed in Săcălăşeni (11.53±0.33) (mg/L) (μg/L). Cd in milk did not show very large variations between localities, so the lowest concentrations were recorded in Săcălăşeni (4.32±0.13) and the highest ones were observed in the locality Firiza (10.93±0.39) (μg/L). The highest concentrations were observed for the Zn in milk, with an interval between (2255.20±7.14) (μg/L) in Săcălăşeni and (3855.80±5.11) (μg/L) for the analyzed samples from Firiza. Keyword: milk, fat, proteins, Zn, Cd, Cu, Pb.
{"title":"Physical–chemical parameters and the level of heavy metals in cow milk in the Baia Mare area","authors":"A. Coroian, V. Mireșan, D. Cocan, C. Răducu, A. Longodor, A. Pop, Gratian Feher, Luisa Andronie, Z. Marchiș","doi":"10.7904/2068-4738-VIII(16)-69","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-69","url":null,"abstract":"Heavy metals are important pollutants in the environment and can cause problems for the organisms, and the bioaccumulation of heavy metals in the food chain can have adverse effects on human health. Regulations on the legally accepted amounts of heavy metals in food and in the body are very precise. The purpose of this paper was to evaluate the level of heavy metals in cow milk from different localities in the Baia Mare area, considered a very polluted area. The physical–chemical parameters and heavy metals in milk were determined. The determination of heavy metals in milk was performed by inductively coupled plasma mass spectrometry or ICP– MS used to identify and quantify the Pb, Cd, Cu, and Zn elements. The highest concentrations of Pb were observed in samples collected in Firiza locality with an average value of (43.22±0.62) (μg/L) and the lowest concentrations are for the samples analyzed in Săcălăşeni (11.53±0.33) (mg/L) (μg/L). Cd in milk did not show very large variations between localities, so the lowest concentrations were recorded in Săcălăşeni (4.32±0.13) and the highest ones were observed in the locality Firiza (10.93±0.39) (μg/L). The highest concentrations were observed for the Zn in milk, with an interval between (2255.20±7.14) (μg/L) in Săcălăşeni and (3855.80±5.11) (μg/L) for the analyzed samples from Firiza. Keyword: milk, fat, proteins, Zn, Cd, Cu, Pb.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"81 1","pages":"69-74"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75420897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-133
Seyed Fayaz Mirasi Oskoee, H. Hamali, R. Asadpour
The objective of this study was to evaluate the efficacy of intravenous infusion of hypertonic glucose for recycling of the cows with postpartum anestrus. One hundred true anestrus Holstein–Frisian cows with ≥ 60 days in milk (DIM) according to the ultrasound examinations were selected from a dairy herd located on the suburb of Tabriz. Anestrus cows randomly were allocated into two groups: A&B (n=50). In group A (Gluco–Select synch), cows received 500 mL hypertonic glucose for 3 consecutive days (–3 to 0) IV, 0.021 mg bucerelin acetate on the day 0, IM followed by 25 mg dinoprost tromethamine IM on the day 7. In group B (Select synch), the same protocol was carried out on the cows except that the glucose hypertonic solution replaced by normal saline as placebo. Thereafter all cows were detected for estrus signs for the next 5 days and inseminated based on visual signs. Twenty seven out of 50 cows (54 %) in group A and 8 out of 50 cows (16 %) in group B showed the estrus signs respectively. Conception rates were recorded as A=44.5 %, B=37.5 % for the first insemination and A=B=100 % for the second insemination of cows. The number of insemination per pregnancy was calculated as 1.55 and 1.62 for groups A and B respectively. In the case of estrus rate, the difference between two groups was statistically significant (P≤0/01), but in the cases of conception rate or the number of insemination per pregnancy rate the differences were not statistically significant. Keyword: Anestrus, Cow, Glucose, Hypertonic, Postpartum.
{"title":"Effect of Intravenous Infusion of Hypertonic Glucose on the Treatment of Cows with Postpartum Anestrus","authors":"Seyed Fayaz Mirasi Oskoee, H. Hamali, R. Asadpour","doi":"10.7904/2068-4738-VIII(16)-133","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-133","url":null,"abstract":"The objective of this study was to evaluate the efficacy of intravenous infusion of hypertonic glucose for recycling of the cows with postpartum anestrus. One hundred true anestrus Holstein–Frisian cows with ≥ 60 days in milk (DIM) according to the ultrasound examinations were selected from a dairy herd located on the suburb of Tabriz. Anestrus cows randomly were allocated into two groups: A&B (n=50). In group A (Gluco–Select synch), cows received 500 mL hypertonic glucose for 3 consecutive days (–3 to 0) IV, 0.021 mg bucerelin acetate on the day 0, IM followed by 25 mg dinoprost tromethamine IM on the day 7. In group B (Select synch), the same protocol was carried out on the cows except that the glucose hypertonic solution replaced by normal saline as placebo. Thereafter all cows were detected for estrus signs for the next 5 days and inseminated based on visual signs. Twenty seven out of 50 cows (54 %) in group A and 8 out of 50 cows (16 %) in group B showed the estrus signs respectively. Conception rates were recorded as A=44.5 %, B=37.5 % for the first insemination and A=B=100 % for the second insemination of cows. The number of insemination per pregnancy was calculated as 1.55 and 1.62 for groups A and B respectively. In the case of estrus rate, the difference between two groups was statistically significant (P≤0/01), but in the cases of conception rate or the number of insemination per pregnancy rate the differences were not statistically significant. Keyword: Anestrus, Cow, Glucose, Hypertonic, Postpartum.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"98 1","pages":"133-139"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84622734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-140
M. Aramesh, Hatef Ajoudanifar
Although several microorganisms are known to produce proteases, Bacillus strains are often preferred as major sources for commercial alkaline proteases due to their exceptional ability to secrete large amounts of highly active enzymes. The present study aimed at isolation and molecular identification of alkaline protease producing Bacillus spp. from soil samples of Damghan County in Iran. This research was conducted in the laboratory complexes in the school of pharmacy of Islamic Azad University Damghan Branch. Soil samples were collected from 3 different regions. Streaking plate was done and 9 colonies were selected on the basis of appearance. To identify isolates, biochemical tests were performed. 16SrRNA gene duplicated with PCR and sent to Sinagene Company for sequencing. All isolates were oxidase–negative and catalase–positive. The ONPG tests were negative. 16SrRNA analysis showed that the isolated bacteria belonged to genus Bacillus. The results confirmed that four bacterial isolates had alkaline protease production capacity. It appears that after complementary examinations, the isolated Bacillus strains can be used for production of alkaline protease. Keyword: alkaline protease, Bacillus, enzyme, biochemical tests.
{"title":"Alkaline protease producing Bacillus isolation and identification from Iran","authors":"M. Aramesh, Hatef Ajoudanifar","doi":"10.7904/2068-4738-VIII(16)-140","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-140","url":null,"abstract":"Although several microorganisms are known to produce proteases, Bacillus strains are often preferred as major sources for commercial alkaline proteases due to their exceptional ability to secrete large amounts of highly active enzymes. The present study aimed at isolation and molecular identification of alkaline protease producing Bacillus spp. from soil samples of Damghan County in Iran. This research was conducted in the laboratory complexes in the school of pharmacy of Islamic Azad University Damghan Branch. Soil samples were collected from 3 different regions. Streaking plate was done and 9 colonies were selected on the basis of appearance. To identify isolates, biochemical tests were performed. 16SrRNA gene duplicated with PCR and sent to Sinagene Company for sequencing. All isolates were oxidase–negative and catalase–positive. The ONPG tests were negative. 16SrRNA analysis showed that the isolated bacteria belonged to genus Bacillus. The results confirmed that four bacterial isolates had alkaline protease production capacity. It appears that after complementary examinations, the isolated Bacillus strains can be used for production of alkaline protease. Keyword: alkaline protease, Bacillus, enzyme, biochemical tests.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":"50 1","pages":"140-147"},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79211516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：