Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献

In this study the small interfering RNA(siRNA) targeting human VEGF effectively inhibited the expression of VEGF in human hepatoma cell line, SMMC7721, and could dramatically decrease the tumorigenicity of SMMC7721 s.c. xenograft tumor. Chemically synthesized siRNA targeting VEGF was transiently transfected into SMMC7721 cells by lipofectamine, RT-PCR and Elisa analysis suggested that the expression of VEGF mRNA and secreted protein in SMMC7721 cells with VEGF siRNA transfection were suppressed with inhibition rates of 76.16% and 96.28% respectively compared with negative control, but the growth rate of SMMC 7721 cells with VEGF siRNA transfection was the same as the control cells. In vivo test, siRNA was injected directly into implanted tumors and the tumors volume were calculated at different time interval. Result showed that VEGF siRNA greatly inhibited the growth of tumors tissues, which was consistent with decrease of VEGF mRNA and protein compared with control. In addition, the VEGF siRNA-treated group exhibited obvious signs of necrosis compared with control.

The effect of galanin (GAL) on neural proliferation was studied in this article using PC12 and B104 cells. RT-PCR was used to determine the expression of galanin and its receptors in both cells. MTT analysis method was employed to detect the effects of galanin and the agonist, antagonist of galanin receptors on the proliferation of both cells. Results showed that PC12 cells expressed mRNAs for all the three galanin receptors (GalRs) but not for galanin, while B104 cells expressed galanin, GalR2, GalR3 except GalR1. In addition, galanin and two receptor agonists, GAL-11 and GAL2-11, could inhibit the proliferation of PC12 cells but activated the proliferation of B104 cells significantly. Moreover, the influences could be blocked by M35, a nonspecific antagonist of galanin receptors. It suggested that GAL can affect cell proliferation via GalRs, but the different galanin receptors might mediate different cell functions.

To investigate the immunocontraception effects of different kinds of mice and seek the relatively effective species-specific DNA vaccines, we constructed four corresponding immunocontraceptive recombinant plasmids: pcDNA3-mzp3 (pcD-M), pcDNA3-Izp3 (pcD-L), pcDNA3-aat-comp-mzp3 (pcD-ACM) and pcDNA3-aat-comp-lzp3 (pcD-ACL) using zona pellucida 3 gene of Lagurus lagurus (Lzp3) and Mus musculus (Mzp3) respectively to immunize NIH mice. With the introduction of hydrnamic transfection instead of traditional Hela cell transfection to study the genes expression in vivo, the results indicated that all four recombinants could be expressed in livers of mice; Histogram pattern of ELISA showed that all of the recombinants in mice could elicit high quantity and lasting specific anti-ZP3 antibody; Antifertility experiment showed that Mzp3 and Lzp3 groups both enhanced sterile effects (P < 0.05), especially the group of pcD-ACM had a significant difference compared with control group (P < 0.01). Histology of ovarian sections demonstrated that pcD-M and pcD-L groups had no disruption of follicular development while pcD-ACL and pcD-ACM did the opposite. The present studies proved that L. lagurus zona pellucida 3 gene (Lzp3) and M. musculus zona pellucida 3 gene (Mzp3) had immunocontraception effects and primarily presumed that they didn't possess species specificity.

In order to evaluate the genotype of CAST gene and its relationship with the muscle histology and other postmortem traits. 158 Jinpi F2 pigs were electrically stunned and exsanguinated. The blood and muscle samples were collected, and both postmortem and meat traits were recorded. PCR-RFLP, PAS and myosin heavy chain immunohistochemistry were employed to explore the relationship between the genotypes and the muscle histology. Based on the PAS reactivity, the muscle fibers can be divided into three types: I, II-a and II-b. Myosin heavy chain immunohistochemistry could differentiated the fibers into slow fibers and fast fibers, the ratio of slow fibers and fast fibers is about 7.62% and 92.38% respectively. The amplification products of the region between 6th and 7th exon of CAST gene were digested by HinfI and discriminated 2 polymorphic sites (524bp and 632bp) in endonuclease map. Only 3 genotypes (AA,AB and BB) was distinguished out. The frequency of AA, AB and BB is 0.1795, 0.5897 and 0.2308 respectively. The frequency of A and B is 0.4743 and 0.5256 respectively. Different genotypes had no statistical significant effect on area, water holding capacity, pH value, and conductivity of longissimus dorsi muscle. The results revealed that the genotypes had a significant effect on the aspect ratio of muscle and showed a negative correlation with the percentage of intramuscular connective tissue.

Tetraploid sorghum (Sorghum bicolor L. Moench) line "sishentian" and Johnsongrass (Sorghum halepense L. Pers) were used to analyze genetic differences between Sorghum and Johnsongrass by SSR (simple sequence repeat) markers and cytogenetic methods. The SSR analyzed results indicated: (1) There were great genetic differences between sorghum and Johnsongrass, According to the different locus distribution, the chromosome linkage groups can be separated into two groups: High differences group and low differences group. (2) Cytogenetic analysis revealed that the parents and their hybrid are irregular tetraploid genetic populations; The chromosome configuration at MI were mainly bivalent and quadrivalents in sorghum, Johnsongrass and their hybrid; there were 17.00, 15.23, 15.83 bivalents and 0.95, 2.15, 1.60 quadrivalents in hybrid, sorghum and Johnsongrass respectively; The results of SSR and cytogenetic analysis demonstrated that the genome of Johnsongrass and Sorghum are homologous in a certain extent. The hybrid can not be steadily hereditary as double diploid.

The aim of this study was to investigate whether ova of Sannen goat could support the pre-implantation development of interspecies embryos constructed through somatic cell nucleus transfer (SCNT) embryos and whether secondary SCNT (SSCNT) could improve the pre-implantation development of those embryos. The primary SCNT (PSCNT) embryos were produced by using Sannen goat ovum cytoplasts as recipients and fibroblast cells, derived from human, rabbit and Boer goat skins, as nucleus donors. The blastomeres of 8 to 16 cells stage of PSCNT embryos were subsequently used as nucleus donor cells and Sannen goat ovum cytoplasts as recipients to evaluate the effect of SSCNT on the pre-implantation development rate of these reconstructed interspecies embryos. Our results indicate that the pre-implantation development rates of SSCNT embryos reconstructed using these three different blastomeres are almost twice of that of corresponding PSCNT embryos (human, 15.8% vs. 7.8%; rabbit, 27.9% vs. 12.5%; Boer goat 55.3% vs. 24.5%; P < 0.05 in all three cases). The time durations that embryos need for the serial events of remodeling and reprogramming to take place vary, depending on the animal species of nucleus donors. These data suggest that remodeling and reprogramming of donor nucleus may be enhanced by prolonged exposure of donor nucleus to maternal cytoplast. We conclude that Sannen goat cytoplast can support the pre-implantation development of embryos constructed with nuclei from various donors, including fibroblasts of human, rabbit and Boer goat; and the somatic nucleus derived from different species requires more time to achieve its reprogramming necessary for pre-implantation development.

A recombinant inbred (RI) population derived from the cross between an indica variety, IR24, and a japonica variety, Asominori, was used to map QTLs controlling leaf chlorophyll content (LCC) and chlorophyll degradation speed (CDS) of detached leaves collected at the tillering stage of rice through composite interval mapping analysis (CIM). Resultantly, four QTLs (qLCC-3, qLCC-5, qLCC-7, qLCC-12) controlling LCC and three QTLs (qCDS-1, qCDS-6, qCDS-7) associated with CDS were detected respectively. Among them, qCDS-7 with the largest effect for CDS, located on chromosome 7, coincided with the genomic region of qLCC-7 for LCC detected. Those results from this study basically can explicate the genetic basis associated with leaf chlorophyll content and chlorophyll degradation speed of detached leaves in rice, which might be available for a rapid determination of leaf senescence at early growth-stages and breeding of high-photosynthetic efficiency in rice.

In order to understand the cytological mechanism of pollen abortion of genetic male sterile mutant induced by space flight in maize, the sister cross population were used for sterility analysis and cytological observation. Intact anther observation, isolated cells observation and paraffin section were adopted in this research. The results showed that pollen abortion occured mostly in dyad stage of meiosis in genetic male sterile mutant. The dyad were degenerated with abnormal shape. In late anther developing stage, the tapetal cells were giant vacuolated and delayed degeneration. The pollen mother cells (PMC) began to dissolve and degenerate in a few anther before meiosis.

Mapping of silkworm Bmrbp1 gene in silkworm chromosome may lay the basis to investigate function of silkworm Bmrbp1 gene and to establish silkworm physical genetic map. In this study, FISH (fluorescence in situ hybridization) was carried out on meiotic chromosome using one sex regulation gene rbp1 of Bombyx mori as a probe. The experimental results demonstrated that: this gene was a single-copy gene of autosome, located at the site with relative length of 38.806 +/- 1.080% in the 5th chromosome of silkworm meiosis.

Using Agrobacterium tumefaciens strain EHA105 carrying binary vector p1301, which containing the gus and hpt genes, a highly efficient transformation system was developed based on the study of factors influencing the Agrobacterium-mediated cotyledonary-node transformation of soybean. The results demonstrated the additions of acetosyringone (200 micromol/L) and ascorbatic acid (50 mg/L) in both infection medium and co-cultivation medium resulted in a significant increase in the transformation efficiency. The induction of the shoots was benefited from the combined utilization of Carbnicillin (250 mg/L) and Cefotaxime (100 mg/L). The inclusion of a 7-d resting step made the selection scheme using hygromycin B as the selective agent more efficient to produce transgenic shoots. Using the optimized transformation procedure, three soybean cultivars widely grown in North China were successfully transformed and the frequency of PCR-positive plant ranged from 3.8%-7.6%. The integration of the transgenes into the soybean nuclear genome was confirmed by PCR analysis using gus- and hpt-specific primers and by Southern blot using hpt-specific probe.