Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献

For studying the role of CRF in the process of depression, chronic intra-cerebral ventricular administration of CRF in normal rats for 21 days were performed and compared with the depression model of chronic unpredictable miled stress (CUMS) in rats. The Open-field Test and Morris water Maze were employed to test the ability of locomotion and learning and memory. HPLC-UV, RT-PCR were employed to analyze the level of blood serum cortisol and the expression of CRF as well as its receptors (R1 and R2) of CUMS models. After chronic stress, the locomotion activity and spatial learning and memory ability decreased obviously, while the level of serum cortisol increased evidently, and the CRF and its receptor-1 mRNA levels were higher compared with those in normal rats. The rats with chronic administration of CRF also consistently decreased the weight gain, locomotion activity and the ability of spatial learning and memory as the CUMS model. This work demonstrates that CRF plays a very important role in the depression genesis and development, sustained elevation of CRF induced by stress may be the chief factor for depression.

The microtubular organelles in the cortical ciliature of the soil ciliate Territricha stramenticola were visualized by direct fluorescent labeling of FLUTAX and immuno-fluorecent labeling using anti-alpha tubulin antibody. The distribution pattern of base-associated microtubules were found to be in accordance with that of adoral zone membranelles (AZM), undulating membranes (UM), frontal-ventral-transverse cirri (FVTC), left-right marginal cirri (L-RMC) and dorsal kineties (DK). The cytoskeleton of AZM and UM was composed of membranelle brackets, membranelle-associated microtubules and the skeleton of UM. The anterior longitudinal microtubules (ALM), posterior longitudinal microtubules (PLM) and transverse microtubules (TM) were located in the basal part of FVTC. ALM, PLM, TM and sprouted microtubules (SM) were situated in the basal parts of LMC and RMC. ALM and PLM were visualized in the basal part of DK. The base of transverse cirri (TC) contained comparatively developed bundles of TM. The occurence of SM and the location of TM in the basal parts of L-RMC might be of species specificity. The microtubules and a layer of microtubule bellow cell's cortex could be used to combine the microtubular cytoskeleton network with cell's various ciliatures used for movement. The presence of these microtubular structures might be one of the cell's adaptive characteristics to soil environment. The microtubules of old AZM were found to be renewed progressively during morphogenesis. The microtubular organelles of old ciliatures might play an important role in the location and substance supply of new structures when these structures begin to occur and develope. During the period of defferetiation and maturation of the new structures, the old ciliatures seemed to play a similar role when they gradually desintegrated and disappeared.

Vibrio alginolyticus is the main Vibrio pathogen in aquaculture in the south of China. A one step loop-mediated isothermal amplification (LAMP) assay was developed for detection of Vibrio alginolyticus. A set of primers were designed from the OmpK sequence of Vibrio alginolyticus. The assay was optimised to amplify Vibrio alginolyticus DNA by incubation at 65 degrees C for only 1 h, and required only a simple water bath or heating block to provide a constant temperature of 65 degrees C. LAMP amplification products had a ladder-like appearance when electrophoresed on an agarose gel. The detection limit of the LAMP assay was n(cell) = 38/mL which was found to be higher than the commonly used PCR method. The assay was evaluated using clinical samples and the results indicated the suitability and simplicity of the test as a rapid, field diagnostic tool for Vibrio alginolyticus.

The present study was performed to explore learning and memory ability in the depression models of rats. The chronic unpredictable stress and olfactory bulbectomy model of rats were adopted. Open-field test was used to detect the locomotion activity and HPLC-UV was employed to analyze the level of blood serum cortisol. The method of Morris water Maze was used to measure learning and memory ability and the results of LTP and LTD in hippocampus CA1 were recorded to observe the synaptic plasticity of hippocampus neurons. The results showed that compared with control group, the locomotion activity and learning ability for two models decreased extremely, while there was no apparent difference in the feedback of memory. Meanwhile, the synaptic plasticity of hippocampus neurons for two models decreased significantly and the level of serum cortisol increased evidently. These results suggested that both methods employed to build the models could cause rats depression and learning inhibition, but do no effects on the feedback of memory.

Gravitropic bending of horizontally-oriented rice shoots results from the differential elongation of cells in the upper and lower halves of the shoot bases. In this study, genes encoding a reversibly glycosylated polypeptide (OsRGP1) and a sucrose synthase (OsSuS) related to sugar metabolism were identified by suppressive subtractive hybridization (SSH) as being differentially expressed in gravibending lower and upper halves of rice shoot bases. RT-PCR was used to monitor OsRGP1 and OsSuS gene expression. The two genes were differentially induced in lower and upper halves of the shoot bases during gravitropism and their expression was regulated by auxin. Gene promoter sequence analysis demonstrated the existence of elements related to auxin. Treatment with the auxin transport inhibitor TIBA inhibited the asymmetric expression of OsRGP1 and OsSuS. In addition, an increase in hexose sugars was detected in the lower half of the shoot bases during gravitropism. Our data suggest that asymmetric redistribution of auxin following gravistimulation results in differentially localized OsRGP1 and OsSuS expression. While asymmetric expression of OsSuS may result in a disproportionate distribution of hexose, asymmetric expression of OsRGP1 could induce cell wall polysaccharide synthesis in the lower half of shoot bases. Therefore hexose and cell wall polysaccharide accumulation in the lower half of rice shoot bases might contribute to cell expansion and subsequent gravitropic bending.

Rab family proteins play a crucial role in regulating vesicular traffic in eukaryotic cells. EoRab43 is an atypical Rab gene identified in Euplotes octocarinatus. In order to understand the function of EoRab43, the 153bp fragment of the 3'-end of EoRab43 gene was subcloned into expression vector pGEX-6P-1, and the recombinant plasmid pGEX-EoRab43(153bp) was transfered into E.coli BL21 (DE3) to express the fusion protein. The fusion protein GST-EoRab43C was expressed and purified by affinity chromatography. BALB/c mice were immunolized by purified GST-EoRab43C. The titer of anti-EoRab43C polyclonal antibody was detected by indirect ELISA assay and the specificity of the antibody was detected by Western blot. Immunofluorescence experiments were performed using anti-EoRab43C antibody in the cells of Euplotes. The results showed that EoRab43 displayed a punctuate pattern in the cytoplasm around the macronuclear chromosome of Euplotes.

On purpose of expounding the phenotype change of Trichoderma mutant which produces cellulase, protein profiles of T. koningii CICC and its mutant TK-2R-1 were detected by 2-DE. Eight unique spots were found and four of them were identified and their functions by MS-TOF-TOF were connected with growth and metabolization of Trichoderma spp. The research provides a new theoretical basis and methods for the construction of Trichoderma mutant which is used to produce efficient cellulase.

SDAU18, an amphiploid of Ae.ventricosa with Ae.cylindrica, was identified by cytological analysis, seed storage protein electrophoresis, genomic in situ hybridization (GISH) and inoculation assessment. The results are as follows: The chromosome number of root tip cells (RTCs) of SDAU18 plants varied from 52 to 56. 28 bivalents were observed in most PMCs MI of SDAU18 with 56 chromosomes, meanwhile, a few univalents, multivalents also existed in some PMCs MI, and the average chromosome configuration was 2n = 56 = 3.21 I +19.78 II, (Ring)+6.50 II (Rod)+0.01 III +0.04 IV (Ring)R+0.01 IV (Rod). There were both Ae. ventricosa-specific bands and Ae. cylindrica-specific bands in the seed storage protein electrophoretogram of SDAU18, furthermore, SDAU18 had one novel HMW-GS not found in the parents and two novel ones not found in common wheats. By labeling the total genomic DNA of Ae. ventricosa and Ae. cylindrica as probes respectively, and using that of another parent as block, GISH of RTCs spread of SDAU18 was carried out. The green hybridization signal was observed in 14 chromosomes respectively, within 56 ones in RTCs of SDAU18. SDAU18 was immune to powdery mildew and stripe rusts. SDAU18 was an amphiploid of Ae. ventricosa with Ae. cylindrica, and had very important significance in wheat breeding and genetic improvement.

Anatomical, histochemical and phytochemistry methods were used to investigate the structure of vegetative organs, and saponins localization and dynamic changes in Polygala sibirica L. The root consisted of developed periderm and secondary vascular. The secondary phloem was thick, and mainly composed of parenchyma. There were well-developed vessels and fibers in the secondary xylem. The stem was composed of epidermis, cortex and vascular bundle. The ring of sclerenchymatous cells lied between cortex and phloem might be the apoplastic protective screen which could protect the stem from drought. The leaf was bifacial one. The root and stem possessed characteristics adapting to arid environment. Histochemical localization results showed that saponins distributed in secondary phloem and phelloderm of root, in epidermis, cortex and phloem of stem, mainly in mesophyll of leaf. It displayed that saponins accumulated mainly in parenchyma cells of vegetative organs, among of which, the secondary phloem was the main storage site. The HPLC results also showed that the saponins accumulated in all the vegetative organs of Polygala sibirica L., with higher content in roots and lower content in the aerial part that included stems and leaves. The study indicated the aerial part of Polygala sibirica L. also had medicinal value. The saponins content had dynamic variance at the developmental stage, the crude drug should be gathered at period from April to May.

A genome-wide analysis of P450 genes was performed in waterflea genome based on their mRNA and amino acid data. Seventy-one P450 genes, assigned into 15 gene families and 17 subfamilies, were identified. They were partitioned into 12 groups and 16 orphan genes by a criterion that the genes belong to the same group when their amino acid identity was more than 60%. Except orphan genes and 4 gene groups, including 2 genes per group, positive selection and gene conversion were analyzed in other 8 groups, including 47 genes. The results showed that positive selection was detected in 3 groups (including 22 genes). The positively selected sites 228E or 277T, locating in SRS (substrate recognition sites) 2 or SRS 5, were detected in 2 groups (18 sequences) with a more than 95% probability, and gene conversions were detected in 5 groups (19 sequences). Interestingly, both positive selection and gene conversion events were identified obviously in 12 genes, and neither positive selection nor gene conversion event was detected in 18 genes. The correlation was revealed between positive selection and gene conversion: positive selection sites were identified in 63.2% of genes in which gene conversion events were detected, and gene conversion events were identified in 54.5% of genes in which positive selections were detected. Moreover, twenty motifs were identified, there in to, 5 motifs existed in over 90% of P450 genes.