PMC biophysics最新文献

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The debut of PMC Biophysics. PMC生物物理学的首次亮相。

PMC biophysics

Pub Date : 2008-11-05 DOI: 10.1186/1757-5036-1-1

Huan-Xiang Zhou

引用次数: 7

ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations. ATR-FTIR光谱检测亚细胞毒性/-基因毒性浓度的MCF-7细胞中有机锡(IV)羧酸盐诱导的变化。

PMC biophysics

Pub Date : 2008-11-05 DOI: 10.1186/1757-5036-1-3

Muhammad S Ahmad, Bushra Mirza, Mukhtiar Hussain, Muhammad Hanif, Saqib Ali, Michael J Walsh, Francis L Martin

The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 mug/ml, 0.1 mug/ml or 1.0 mug/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-strand breaks (SSBs) and the cytokinesis-block micronucleus (CBMN) assay to determine micronucleus formation. Biochemical-cell signatures were also ascertained using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. In the comet assay, organotin(IV) carboxylates induced significantly-elevated levels of DNA SSBs. Elevated micronucleus-forming activities were also observed. Following interrogation using ATR-FTIR spectroscopy, infrared spectra in the biomolecular range (900 cm-1 - 1800 cm-1) derived from organotin-treated MCF-7 cells exhibited clear alterations in their biochemical-cell fingerprint compared to control-cell populations following exposures as low as 0.0001 mug/ml. Mono-, di- or tri-organotin(IV) carboxylates (0.1 mug/ml, 1.0 mug/ml or 10.0 mug/ml) were markedly cytotoxic as determined by the clonogenic assay following treatment of MCF-7 cells with >/= 1.0 mug/ml. Our results demonstrate that ATR-FTIR spectroscopy can be applied to detect molecular alterations induced by organotin(IV) compounds at sub-cytotoxic and sub-genotoxic concentrations. This biophysical approach points to a novel means of assessing risk associated with environmental contaminants.PACS codes: 87.15.-v, 87.17.-d, 87.18.-h.

金属配合物如有机锡(IV)化合物对环境的影响日益受到关注。采用碱性单细胞凝胶电泳(comet)法测定DNA单链断裂(SSBs)，细胞分裂阻断微核(CBMN)法测定微核形成，测定有机锡(IV)化合物(0.01杯/ml、0.1杯/ml或1.0杯/ml)的遗传毒性效应。利用衰减全反射傅里叶变换红外光谱(ATR-FTIR)确定了生化细胞的特征。在彗星试验中，有机锡(IV)羧酸盐诱导DNA SSBs水平显著升高。微核形成活性升高也被观察到。在使用ATR-FTIR光谱分析后，有机锡处理的MCF-7细胞的生物分子范围(900 cm-1 - 1800 cm-1)的红外光谱显示，与对照细胞群体相比，在暴露低至0.0001马克杯/毫升时，其生化细胞指纹发生了明显的变化。单、二或三有机锡(IV)羧酸盐(0.1马克杯/毫升、1.0马克杯/毫升或10.0马克杯/毫升)在>/= 1.0马克杯/毫升的MCF-7细胞处理后，通过克隆生成实验发现，它们具有明显的细胞毒性。我们的研究结果表明，ATR-FTIR光谱可以用于检测亚细胞毒性和亚基因毒性浓度的有机锡(IV)化合物引起的分子改变。这种生物物理方法指出了一种评估与环境污染物相关风险的新方法。PACS代码:87.15。- v, 87.17。- d, 87.18.-h。

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引用次数: 19

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