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Calcium phosphate-hybridized tendon graft to enhance tendon-bone healing two years after ACL reconstruction in goats. 磷酸钙杂交肌腱移植物促进山羊前交叉韧带重建两年后肌腱骨愈合。
Pub Date : 2011-12-14 DOI: 10.1186/1758-2555-3-31
Hirotaka Mutsuzaki, Masataka Sakane

Background: We developed a novel technique to improve tendon-bone attachment by hybridizing calcium phosphate (CaP) with a tendon graft using an alternate soaking process. However, the long-term result with regard to the interface between the tendon graft and the bone is unclear.

Methods: We analyzed bone tunnel enlargement by computed tomography and histological observation of the interface and the tendon graft with and without the CaP hybridization 2 years after anterior cruciate ligament (ACL) reconstruction in goats using EndoButton and the postscrew technique (CaP, n = 4; control, n = 4).

Results: The tibial bone tunnel enlargement rates in the CaP group were lower than those in the control group (p < 0.05). In the CaP group, in the femoral and tibial bone tunnels at the anterior and posterior of the joint aperture site, direct insertion-like formation that contained a cartilage layer without tidemarks was more observed at the tendon-bone interface than in the control group (p < 0.05). Moreover, the gap area between the tendon graft and the bone was more observed at the femoral bone tunnel of the joint aperture site in the control group than in the CaP group (p < 0.05). The maturation of the tendon grafts determined using the ligament tissue maturation index was similar in both groups.

Conclusions: The CaP-hybridized tendon graft enhanced the tendon-bone healing 2 years after ACL reconstruction in goats. The use of CaP-hybridized tendon grafts can reduce the bone tunnel enlargement and gap area associated with the direct insertion-like formation in the interface near the joint.

背景:我们开发了一种新技术,通过交替浸泡过程将磷酸钙(CaP)与肌腱移植物杂交来改善肌腱-骨附着。然而,关于肌腱移植物与骨之间的界面的长期结果尚不清楚。方法:应用EndoButton和螺钉后技术(CaP, n = 4;结果:CaP组胫骨隧道扩大率明显低于对照组(p < 0.05)。CaP组在关节孔前后的股骨和胫骨隧道中,在肌腱-骨界面处观察到更多含有软骨层且无潮痕的直接插入样形成(p < 0.05)。与CaP组相比,对照组在关节孔处股骨隧道处肌腱移植物与骨之间的间隙面积更大(p < 0.05)。用韧带组织成熟指数测定两组肌腱移植的成熟程度相似。结论:cap杂交肌腱移植可促进山羊前交叉韧带重建2年后的肌腱骨愈合。使用cap杂交肌腱移植物可以减少与关节附近界面直接插入样形成相关的骨隧道扩大和间隙面积。
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引用次数: 22
Double bundle arthroscopic Anterior Cruciate Ligament reconstruction with remnant preserving technique using a hamstring autograft. 双束关节镜下腘绳肌腱自体移植物保留残馀技术重建前交叉韧带。
Pub Date : 2011-12-05 DOI: 10.1186/1758-2555-3-30
Mitsuo Ochi, Mohamed M Abouheif, Wirat Kongcharoensombat, Atsuo Nakamae, Nobuo Adachi, Masataka Deie

Background: Preservation of the Anterior Cruciate Ligament (ACL) remnant is important from the biological point of view as it enhances revascularization, and preserves the proprioceptive function of the graft construct. Additionally, it may have a useful biomechanical function. Double bundle ACL reconstruction has been shown to better replicate the native ACL anatomy and results in better restoration of the rotational stability than single bundle reconstruction.

Methods: We used the far anteromedial (FAM) portal for creation of the femoral tunnels, with a special technique for its preoperative localization using three dimensional (3D) CT. The central anteromedial (AM) portal was used to make a longitudinal slit in the ACL remnant to allow visualization of the tips of the guide pins during anatomical creation of the tibial tunnels within the native ACL tibial foot print. The use of curved hemostat allow retrieval of the wire loop from the apertures of the femoral tunnels through the longitudinal slit in the ACL remnant thereby, guarding against impingement of the reconstruction graft against the ACL remnant as well as the roof of the intercondylar notch.

Conclusion: Our technique allows for anatomical double bundle reconstruction of the ACL while maximally preserving the ACL remnant without the use of intra-operative image intensifier.

背景:从生物学的角度来看,保存前交叉韧带(ACL)残余物是重要的,因为它增强了血管重建,并保留了移植物结构的本体感觉功能。此外,它可能具有有用的生物力学功能。与单束重建相比,双束ACL重建能更好地复制原ACL解剖结构,并能更好地恢复旋转稳定性。方法:我们使用远前内侧(FAM)门静脉创建股骨隧道,并使用三维CT进行术前定位的特殊技术。使用中央前内侧(AM)门静脉在前交叉韧带残肢上开纵缝,以便在原前交叉韧带胫骨足印内解剖创建胫骨隧道时,能够看到引导钉的尖端。弯曲止血钳的使用允许将钢丝环从股骨隧道的孔中取出,通过残余前交叉韧带的纵缝,从而防止重建移植物撞击残余前交叉韧带以及髁间切迹的顶部。结论:我们的技术允许解剖双束重建前交叉韧带,同时最大限度地保留前交叉韧带残余,而无需使用术中图像增强器。
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引用次数: 38
Application of layered poly (L-lactic acid) cell free scaffold in a rabbit rotator cuff defect model. 层状聚l -乳酸无细胞支架在兔肩袖缺损模型中的应用。
Pub Date : 2011-12-02 DOI: 10.1186/1758-2555-3-29
Atsuyuki Inui, Takeshi Kokubu, Hiroyuki Fujioka, Issei Nagura, Ryosuke Sakata, Hanako Nishimoto, Masaru Kotera, Takashi Nishino, Masahiro Kurosaka

Background: This study evaluated the application of a layered cell free poly (L-lactic acid) (PLLA) scaffold to regenerate an infraspinatus tendon defect in a rabbit model. We hypothesized that PLLA scaffold without cultivated cells would lead to regeneration of tissue with mechanical properties similar to reattached infraspinatus without tendon defects.

Methods: Layered PLLA fabric with a smooth surface on one side and a pile-finished surface on the other side was used. Novel form of layered PLLA scaffold was created by superimposing 2 PLLA fabrics. Defects of the infraspinatus tendon were created in 32 rabbits and the PLLA scaffolds were transplanted, four rabbits were used as normal control. Contralateral infraspinatus tendons were reattached to humeral head without scaffold implantation. Histological and mechanical evaluations were performed at 4, 8, and 16 weeks after operation.

Results: At 4 weeks postoperatively, cell migration was observed in the interstice of the PLLA fibers. Regenerated tissue was directly connected to the bone composed mainly of type III collagen, at 16 weeks postoperatively. The ultimate failure load increased in a time-dependent manner and no statistical difference was seen between normal infraspinatus tendon and scaffold group at 8 and 16 weeks postoperatively. There were no differences between scaffold group and reattach group at each time of point. The stiffness did not improve significantly in both groups.

Conclusions: A novel form of layered PLLA scaffold has the potential to induce cell migration into the scaffold and to bridge the tendon defect with mechanical properties similar to reattached infraspinatus tendon model.

背景:本研究评估了层状无细胞聚l -乳酸(PLLA)支架在兔冈下肌腱缺损模型中的应用。我们假设没有培养细胞的PLLA支架可以再生组织,其机械性能与无肌腱缺损的冈下肌再植相似。方法:采用一边表面光滑,另一边表面绒毛整理的层状PLLA织物。将2块PLLA纤维叠加在一起,形成了一种新型的分层PLLA支架。32只家兔造出冈下肌腱缺损,移植PLLA支架,4只家兔作为正常对照。对侧冈下肌腱在肱骨头上再植,不植入支架。分别于术后4、8、16周进行组织学和力学评价。结果:术后4周,PLLA纤维间质出现细胞迁移。术后16周,以ⅲ型胶原为主的再生组织与骨直接连接。术后8周和16周,正常冈下肌腱组与支架组的最终失效负荷呈时间依赖性增加,差异无统计学意义。各时间点支架组与复接组间无差异。两组患者的僵硬度均无明显改善。结论:一种新型的层状PLLA支架具有诱导细胞向支架内迁移和桥接肌腱缺损的潜力,其力学特性与再附着的甘下肌腱模型相似。
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引用次数: 29
Percutaneous & Mini Invasive Achilles tendon repair. 经皮微创跟腱修复术。
Pub Date : 2011-11-14 DOI: 10.1186/1758-2555-3-28
Michael R Carmont, Roberto Rossi, Sven Scheffler, Omer Mei-Dan, Philippe Beaufils

Rupture of the Achilles tendon is a considerable cause of morbidity with reduced function following injury. Recent studies have shown little difference in outcome between the techniques of open and non-operative treatment using an early active rehabilitation programme. Meta-analyses have shown that non-operative management has increased risk of re-rupture whereas surgical intervention has risks of complications related to the wound and iatrogenic nerve injury. Minimally invasive surgery has been adopted as a way of reducing infections rates and wound breakdown however avoiding iatrogenic nerve injury must be considered. We discuss the techniques and outcomes of percutaneous and minimally invasive repairs of the Achilles tendon.

跟腱断裂是损伤后功能降低的一个重要原因。最近的研究表明,采用早期主动康复方案的开放技术和非手术治疗的结果差别不大。荟萃分析显示,非手术治疗增加了再破裂的风险,而手术干预有与伤口和医源性神经损伤相关的并发症的风险。微创手术已成为降低感染率和伤口破裂的一种方法,但必须考虑避免医源性神经损伤。我们讨论经皮和微创修复跟腱的技术和结果。
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引用次数: 31
Results from the translation and adaptation of the Iranian Short-Form McGill Pain Questionnaire (I-SF-MPQ): preliminary evidence of its reliability, construct validity and sensitivity in an Iranian pain population. 伊朗短形式麦吉尔疼痛问卷(I-SF-MPQ)的翻译和改编结果:其在伊朗疼痛人群中的可靠性、结构效度和敏感性的初步证据。
Pub Date : 2011-11-10 DOI: 10.1186/1758-2555-3-27
Farhad Adelmanesh, Ali Arvantaj, Hassan Rashki, Seyedmehdi Ketabchi, Ali Montazeri, Gholamreza Raissi

Background: The Short Form McGill Pain Questionnaire (SF-MPQ) is one of the most widely used instruments to assess pain. The aim of this study was to translate and culturally adapt the questionnaire for Farsi (the official language of Iran) speakers in order to test its reliability and sensitivity.

Methods: We followed Guillemin's guidelines for cross-cultural adaption of health-related measures, which include forward-backward translations, expert committee meetings, and face validity testing in a pilot group. Subsequently, the questionnaire was administered to a sample of 100 diverse chronic pain patients attending a tertiary pain and rehabilitation clinic. In order to evaluate test-retest reliability, patients completed the questionnaire in the morning and early evening of their first visit. Finally, patients were asked to complete the questionnaire for the third time after completing a standardized treatment protocol three weeks later. Intraclass correlation coefficient (ICC) was used to evaluate reliability. We used principle component analysis to assess construct validity.

Results: Ninety-two subjects completed the questionnaire both in the morning and in the evening of the first visit (test-retest reliability), and after three weeks (sensitivity to change). Eight patients who did not finish treatment protocol were excluded from the study. Internal consistency was found by Cronbach's alpha to be 0.951, 0.832 and 0.840 for sensory, affective and total scores respectively. ICC resulted in 0.906 for sensory, 0.712 for affective and 0.912 for total pain score. Item to subscale score correlations supported the convergent validity of each item to its hypothesized subscale. Correlations were observed to range from r2 = 0.202 to r2 = 0.739. Sensitivity or responsiveness was evaluated by pair t-test, which exhibited a significant difference between pre- and post-treatment scores (p < 0.001).

Conclusion: The results of this study indicate that the Iranian version of the SF-MPQ is a reliable questionnaire and responsive to changes in the subscale and total pain scores in Persian chronic pain patients over time.

背景:短格式麦吉尔疼痛问卷(SF-MPQ)是最广泛使用的疼痛评估工具之一。本研究的目的是为波斯语(伊朗官方语言)使用者翻译问卷并进行文化调整,以测试其可靠性和敏感性。方法:我们遵循Guillemin关于健康相关措施的跨文化适应指南,包括向前向后翻译、专家委员会会议和在试点群体中进行面孔效度测试。随后,对100名在三级疼痛康复诊所就诊的不同类型的慢性疼痛患者进行问卷调查。为了评估重测信度,患者在第一次就诊的早晨和傍晚完成问卷。最后,患者在三周后完成标准化治疗方案后第三次被要求填写问卷。用类内相关系数(Intraclass correlation coefficient, ICC)评价信度。我们使用主成分分析来评估构念效度。结果:92名受试者分别于首次访视的早晚(重测信度)和三周后(变化敏感性)完成问卷。8例未完成治疗方案的患者被排除在研究之外。经Cronbach's alpha检验,感觉、情感和总分的内部一致性分别为0.951、0.832和0.840。结果:感觉疼痛评分为0.906,情感疼痛评分为0.712,总疼痛评分为0.912。项目与子量表得分的相关性支持每个项目与其假设子量表的收敛效度。相关系数r2 = 0.202 ~ r2 = 0.739。敏感度或反应性采用配对t检验,治疗前后评分差异显著(p < 0.001)。结论:本研究结果表明,伊朗版SF-MPQ是一份可靠的问卷,能够反映波斯慢性疼痛患者亚量表和总疼痛评分随时间的变化。
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引用次数: 37
Immunohistological analysis of extracted anterior cruciate ligament graft impinged against posterior cruciate ligament. 取出的前交叉韧带移植物撞击后交叉韧带的免疫组织学分析。
Pub Date : 2011-11-02 DOI: 10.1186/1758-2555-3-26
So Kato, Atsushi Fukai, Hideki Takeda, Shuji Taketomi, Shuichi Nakayama, Jinso Hirota, Kohei Nakajima, Kozo Nakamura, Takumi Nakagawa

A young female athlete suffered from the residual instability of the knee after anterior cruciate ligament (ACL) reconstruction with hamstring autograft. The 3-dimensional (3-D) CT scan showed the "high noon" positioning of the primary femoral bone tunnel. The revision surgery with anatomic double-bundle technique was performed two years after the primary surgery and the femoral tunnels were created with the assistance of the 3-D fluoroscopy-based navigation. An arthroscopic examination confirmed the ACL graft impingement against posterior cruciate ligament (PCL) when the knee was deeply flexed. The histological analysis of the resected primary ACL graft showed local inflammatory infiltration, enhanced synovial coverage and vascularization at the impinged site. The enhanced expression of vascular endothelial growth factor (VEGF) at the impinged area when compared with non-impinged area was observed on immunohistochemical analysis. Abnormal mechanical stress by the impingement against PCL might have induced chronic inflammation and VEGF overexpression.

一位年轻的女运动员在用自体腘绳肌腱重建前交叉韧带(ACL)后出现膝关节残余不稳定。三维(3-D) CT扫描显示原发性股骨隧道的“正午”定位。在初次手术两年后采用解剖双束技术进行翻修手术,并在三维透视导航的帮助下创建了股骨隧道。膝关节深度屈曲时,关节镜检查证实ACL移植物撞击后交叉韧带(PCL)。切除的原发性前交叉韧带移植物的组织学分析显示局部炎症浸润,滑膜覆盖增强,撞击部位血管化。免疫组化观察撞击区血管内皮生长因子(VEGF)表达较非撞击区增强。PCL撞击引起的机械应力异常可能引起慢性炎症和VEGF过表达。
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引用次数: 1
Clinical examination of the knee: know your tools for diagnosis of knee injuries. 膝关节的临床检查:了解诊断膝关节损伤的工具。
Pub Date : 2011-10-28 DOI: 10.1186/1758-2555-3-25
Roberto Rossi, Federico Dettoni, Matteo Bruzzone, Umberto Cottino, Davide G D'Elicio, Davide E Bonasia

The clinical evaluation of the knee is a fundamental tool to correctly address diagnosis and treatment, and should never be replaced by the findings retrieved by the imaging studies carried on the patient.Every surgeon has his own series of exams with whom he is more confident and on whom he relies on for diagnosis. Usually, three sets of series are used: one for patello-femoral/extensor mechanism pathologies; one for meniscal and chondral (articular) lesions; and one for instability evaluation.This review analyses the most commonly used tests and signs for knee examination, outlining the correct way to perform the test, the correct interpretation of a positive test and the best management for evaluating an injured knee both in the acute and delayed timing.

膝关节的临床评估是正确诊断和治疗的基本工具,绝不能被对患者进行的影像学研究所检索到的结果所取代。每个外科医生都有自己的一系列检查,他对这些检查更有信心,也可以依靠这些检查来进行诊断。通常,使用三组系列:一组用于髌股/伸肌机制病理;一个用于半月板和软骨(关节)损伤;一个用于不稳定性评估。这篇综述分析了最常用的膝盖检查测试和体征,概述了正确的测试方法、阳性测试的正确解释以及在急性和延迟时间评估受伤膝盖的最佳管理方法。
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引用次数: 43
Backward walking training improves balance in school-aged boys. 向后走训练可以提高学龄男孩的平衡能力。
Pub Date : 2011-10-22 DOI: 10.1186/1758-2555-3-24
Wei-Ya Hao, Yan Chen

Background: Falls remain a major cause of childhood morbidity and mortality. It is suggested that backward walking (BW) may offer some benefits especially in balance and motor control ability beyond those experienced through forward walking (FW), and may be a potential intervention for prevention of falls. The objective of this study was to investigate the effects of BW on balance in boys.

Methods: Sixteen healthy boys (age: 7.19 ± 0.40 y) were randomly assigned to either an experimental or a control group. The experimental group participated in a BW training program (12-week, 2 times weekly, and 25-min each time) but not the control group. Both groups had five dynamic balance assessments with a Biodex Stability System (anterior/posterior, medial/lateral, and overall balance index) before, during and after the training (week- 0, 4, 8, 12, 24). Six control and six experimental boys participated in a study comparing kinematics of lower limbs between FW and BW after the training (week-12).

Results: The balance of experimental group was better than that of control group after 8 weeks of training (P < 0.01), and was still better than that of control group (P < 0.05), when the BW training program had finished for 12 weeks. The kinematic analysis indicated that there was no difference between control and experimental groups in the kinematics of both FW and BW gaits after the BW training (P > 0.05). Compared to FW, the duration of stance phase of BW tended to be longer, while the swing phase, stride length, walking speed, and moving ranges of the thigh, calf and foot of BW decreased (P < 0.01).

Conclusion: Backward walking training in school-aged boys can improve balance.

背景:跌倒仍然是儿童发病和死亡的主要原因。研究表明,向后行走(BW)可能比向前行走(FW)更有利于平衡和运动控制能力,并可能是预防跌倒的潜在干预措施。本研究的目的是探讨体重对男孩平衡感的影响。方法:16名健康男孩(年龄:7.19±0.40岁)随机分为实验组和对照组。实验组接受体重训练(12周,每周2次,每次25分钟),对照组不接受训练。两组在训练前、训练中和训练后(第0、4、8、12、24周)均使用Biodex稳定性系统(前/后、内/外侧和整体平衡指数)进行了5次动态平衡评估。6名对照男孩和6名实验男孩参加了训练后(第12周)FW和BW下肢运动学的比较研究。结果:试验组在训练8周后平衡性优于对照组(P < 0.01),在训练12周后平衡性仍优于对照组(P < 0.05)。运动学分析表明,对照组和试验组在体重训练后的FW和BW步态的运动学均无显著差异(P > 0.05)。与FW相比,BW的站立阶段持续时间有延长的趋势,而摆动阶段、步幅、步行速度、大腿、小腿和足部的活动范围均减小(P < 0.01)。结论:学龄男孩后行训练可提高平衡性。
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引用次数: 37
From 2D leg kinematics to 3D full-body biomechanics-the past, present and future of scientific analysis of maximal instep kick in soccer. 从2D腿部运动学到3D全身生物力学——足球中最大脚背踢的科学分析的过去、现在和未来。
Pub Date : 2011-10-19 DOI: 10.1186/1758-2555-3-23
Gongbing Shan, Xiang Zhang

Biomechanics investigation on soccer kicking has a relatively long history, yet the body of knowledge is still small. This paper reviews articles published from 1960s to 2011, summarizing relevant findings, research trends and method development. It also discusses challenges faced by the field. The main aim of the paper is to promote soccer kicking studies through discussions on problem solving in the past, method development in the present, and possible research directions for the future.

足球运动的生物力学研究已有较长的历史,但目前的知识体系还很小。本文回顾了20世纪60年代至2011年发表的文章,总结了相关发现、研究趋势和方法发展。它还讨论了该领域面临的挑战。本文的主要目的是通过讨论过去的问题解决,现在的方法发展以及未来可能的研究方向来促进足球踢球研究。
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引用次数: 35
Effect of Body Mass Index on work related musculoskeletal discomfort and occupational stress of computer workers in a developed ergonomic setup. 身体质量指数对电脑工人工作相关肌肉骨骼不适和职业压力的影响。
Pub Date : 2011-10-07 DOI: 10.1186/1758-2555-3-22
Jasobanta Sethi, Jaspal Singh Sandhu, Vijay Imbanathan

Background: Work urgency, accuracy and demands compel the computer professionals to spend longer hours before computers without giving importance to their health, especially body weight. Increase of body weight leads to improper Body Mass Index (BMI) may aggravate work related musculoskeletal discomfort and occupational-psychosocial stress. The objective of the study was to find out the effect of BMI on work related musculoskeletal discomforts and occupational stress of computer workers in a developed ergonomic setup.

Methods: A descriptive inferential study has been taken to analyze the effect of BMI on work related musculoskeletal discomfort and occupational-psychosocial stress. A total of 100 computer workers, aged 25-35 years randomly selected on convenience from software and BPO companies in Bangalore city, India for the participation in this study. BMI was calculated by taking the ratio of the subject's height (in meter) and weight (in kilogram). Work related musculoskeletal discomfort and occupational stress of the subjects was assessed by Cornell University's musculoskeletal discomfort questionnaire (CMDQ) and occupational stress index (OSI) respectively as well as a relationship was checked with their BMI.

Results: A significant association (p < 0.001) was seen among high BMI subjects with their increase scores of musculoskeletal discomfort and occupational stress.

Conclusion: From this study, it has been concluded that, there is a significant effect of BMI in increasing of work related musculoskeletal discomfort and occupational-psychosocial stress among computer workers in a developed ergonomic setup.

背景:工作的紧迫性、准确性和需求迫使计算机专业人员在电脑前花费更长时间,而不重视他们的健康,特别是体重。体重增加导致的身体质量指数(BMI)不合理会加重与工作相关的肌肉骨骼不适和职业心理社会压力。本研究的目的是在一个已发展的人体工程学环境中,找出身体质量指数对电脑工作人员与工作相关的肌肉骨骼不适和职业压力的影响。方法:采用描述性推理研究分析BMI对工作相关肌肉骨骼不适和职业心理社会压力的影响。从印度班加罗尔市的软件和业务流程外包公司中随机抽取了100名年龄在25-35岁之间的计算机从业人员参与本研究。BMI是用受试者的身高(米)和体重(公斤)之比来计算的。分别采用康奈尔大学肌肉骨骼不适问卷(CMDQ)和职业压力指数(OSI)对被试的工作相关肌肉骨骼不适和职业压力进行评估,并与被试的身体质量指数(BMI)进行关系检验。结果:高BMI受试者的肌肉骨骼不适和职业压力得分增加显著相关(p < 0.001)。结论:在发达的人体工程学环境中,BMI对计算机工作者的工作相关肌肉骨骼不适和职业心理社会压力有显著影响。
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引用次数: 62
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Sports medicine, arthroscopy, rehabilitation, therapy & technology : SMARTT
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