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The Coping Long Term with Active Suicide Program: Description and Pilot Data. 长期应对主动自杀计划》:说明和试点数据。
IF 3.2 Pub Date : 2016-12-01 Epub Date: 2016-04-02 DOI: 10.1111/sltb.12247
Ivan W Miller, Brandon A Gaudiano, Lauren M Weinstock

A description of and pilot data for a new adjunctive intervention designed to reduce suicidal behavior among high-risk individuals are presented. The Coping Long Term with Active Suicide Program (CLASP) has three major components: (1) three individual, in-person meetings, (2) one significant other/family meeting, and (3) 11 brief (15-30 minute) phone contacts with the patient and his/her significant other. Open trial and pilot randomized trial data provide preliminary evidence that CLASP is a feasible, acceptable, and efficacious intervention to reduce suicidal behavior.

本文介绍了一种旨在减少高危人群自杀行为的新型辅助干预措施,并提供了相关试验数据。长期应对主动自杀计划(CLASP)有三个主要组成部分:(1) 3 次个人面谈;(2) 1 次重要他人/家人面谈;(3) 11 次与患者及其重要他人的简短(15-30 分钟)电话联系。公开试验和试点随机试验数据初步证明,CLASP 是一种可行、可接受且有效的干预措施,可减少自杀行为。
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引用次数: 0
Inactivation of bacteria by high-power 940 nm laser irradiation 高功率940nm激光照射细菌的灭活
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.004
Martin Udart, Karl Stock, Rainer Graser, Raimund Hibst

Background and objective

Diode lasers are used in dentistry for oral surgery or disinfection of root canals in endodontic treatment. The aim of the study was to elucidate the basic mechanism of action in laser-induced root canal disinfection, which could be a photothermal and/or photochemical effect.

Materials and methods

To find out whether high-power NIR laser bacterial killing is caused by the light itself (photochemical effect or direct bacterial heating by selective absorption) or by a general thermal process, bacterial suspensions of a non-pathogenic strain of Escherichia coli were heated in a water bath and by diode laser (940 nm) each with the same temporal temperature course. Furthermore, bacterial suspensions were irradiated during which the temperature was fixed by iced water. Efficacy of bacterial inactivation was determined using double fluorescence labeling to distinguish vital from dead bacteria.

Results

Comparison of bacteria-killing efficacy between laser and water bath-induced heating showed no significant differences. The most important parameter was the maximum temperature achieved during heating. Laser irradiation of bacteria at low temperatures (elimination of temperature effect) did not show any bactericidal effect.

Conclusion

The experiments show that the inactivation of bacteria, at least for E. coli, using high-power 940 nm laser irradiation is solely based on a thermal mechanism. This should be taken into consideration during laser-assisted endodontic disinfection, where efficient bacterial inactivation must be achieved whilst preserving temperature-sensitive neighboring tissue.

Hintergrund und Zielsetzung

Diodenlaser finden in der Zahnheilkunde im Rahmen der Oralchirurgie und der Desinfektion des Wurzelkanals bei der Wurzelkanalbehandlung Verwendung. Das Ziel unserer Studie war, den grundlegenden Mechanismus der Laser-induzierten Desinfektion des Wurzelkanals zu untersuchen, dem sowohl ein photothermischer als auch ein photochemischer Effekt zugrunde liegen könnte.

Material und Methoden

Um herauszufinden, ob die Inaktivierung von Bakterien durch die Laserstrahlung (photochemischer Effekt oder direkte Erhitzung der Bakterien durch selektive Absorption) hervorgerufen wird oder von allgemein thermischer Natur ist, wurden Bakteriensuspensionen eines nicht-pathogenen Stamms von Escherichia coli in vergleichbarer Weise durch Bestrahlung mit einem Diodenlaser (940 nm) oder im Wasserbad erhitzt. In einem weiteren Experiment wurden die Bakteriensuspensionen während der Bestrahlung in Eiswasser gekühlt, um so ein Ansteigen der Temperatur zu verhindern. Die jeweilige Wirkung auf die Vitalität der Bakterien wurde durch anschließende Färbung mit zwei Fluoreszenzfarbstoffen bestimmt, die die Unterscheidung von lebenden und toten Bakterien

背景与目的二极管激光器用于口腔手术或根管消毒。本研究的目的是阐明激光诱导根管消毒的基本作用机制,这种作用可能是光热和/或光化学作用。材料和方法为了确定高功率近红外激光杀灭细菌是由光本身(光化学效应或通过选择性吸收直接加热细菌)还是由一般的热过程引起的,我们在水浴中加热了一种非致病性大肠杆菌菌株的细菌悬液,并使用二极管激光(940 nm)在相同的时间温度过程中加热。此外,对细菌悬浮液进行辐照,期间用冰水固定温度。用双荧光标记法区分活菌和死菌,确定细菌灭活效果。结果激光与水浴加热杀菌效果比较无显著差异。最重要的参数是加热过程中达到的最高温度。低温激光照射细菌(消除温度效应)没有显示出任何杀菌效果。结论高功率940 nm激光对大肠杆菌的灭活作用完全基于热机制。在激光辅助牙髓消毒过程中,必须考虑到这一点,在保持对温度敏感的邻近组织的同时,必须实现有效的细菌灭活。在口腔外科手术的研究中发现了一种新的研究方法,即在口腔外科的研究中发现了一种新的研究方法,即在口腔外科的研究中发现了一种新的研究方法。Das Ziel unserer研究战争,den grundlegenden Mechanismus der laser - industrizierten Desinfektion des Wurzelkanals zu untersuchen, dem sohl in photothermischer and auch in photochemischer effect zugrunde liegen könnte。材料与方法herauszufinden, ob die Inaktivierung von Bakterien durch die Laserstrahlung(光化学效应oder direcrekte Erhitzung der Bakterien durch选择性吸收)hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgerufen, hervorgensuspensionen eines -致病菌Stamms von Escherichia coli in vergleichbarer Weise durh Bestrahlung mit einem didenlaser (940 nm) oder in Wasserbad erhitzt。In einem weiteren Experiment wurden die Bakteriensuspensionen während der Bestrahlung In Eiswasser gekhlt, um so In Ansteigen der temperature zu verhindern。Die jeweilige Wirkung auf Die Vitalität der bakteren wurche schließende Färbung mit zwei fluoreszenzfarstofoften bestbestmt, Die Die Unterscheidung von lebenden and toten bakteren erlaubt。2 .激光辐照技术在激光辐照条件下的应用Der wichtigste参数ist die durch die Erwärmung erreichte zieltemperature Der Bakteriensuspension。激光对悬浮粒子的影响(Ausschalten des effeters der Temperaturerhöhung)与对悬浮粒子的影响(Ausschalten des effeters der Temperaturerhöhung)研究结果表明:1 .激光辐射对大肠杆菌的影响;2 .激光辐射对大肠杆菌的影响Diese Erkenntnis sollte beder endodontischen desinfetion mittelels Laser beachtet werden, beder eine effizente Inaktivierung von bakteren gewährleistet sein muss, behne benachbartes temperature rempfindlich Gewebe zu schädigen。
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引用次数: 8
Content 内容
Pub Date : 2011-11-01 DOI: 10.1016/S1615-1615(11)00063-9
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引用次数: 0
Satzung der Deutschen Gesellschaft für Lasermedizin e.V. 德国激光协会章程
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.008
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引用次数: 0
Investigation of lipid bodies in a colon carcinoma cell line by confocal Raman microscopy 用共聚焦拉曼显微镜观察结肠癌细胞系的脂质体
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.002
Claudia Scalfi-Happ, Martin Udart, Carmen Hauser, Angelika Rück

Objective

The investigation of living cells under physiological conditions requires sensitive, sophisticated and in particular, non-invasive methods. Raman spectroscopy provides vibrational information about the sample. Combined with high-resolution confocal microscopy, it allows a complete Raman spectrum to be recorded at every confocal image point. This technique was applied here for the investigation of lipid bodies in a colon carcinoma cell line.

Materials and methods

The colorectal adenocarcinoma cell line Caco-2 and the rat intestine epithelial cell line IEC-6 were analysed with the confocal Raman microscope alpha300 R (WITec GmbH, Germany), using a frequency-doubled Nd:YAG laser at 532 nm and 10 mW for excitation. The use of a water immersion lens (63×, NA 1.0) allowed a lateral resolution in the sub-micrometer range. Raman images of cells were generated from the data sets by integrating over specific Raman bands. Mapping of the C–H stretching band (2800–3030 cm−1) allowed for the visualisation of the whole cell, whereas the automated statistical evaluation of all spectra by k-means cluster analysis resulted in spectral unmixed images which provided an insight into the chemical composition of the sample.

Results

With the described method, it was possible to visualise the distribution of different cellular biomarkers. Lipid bodies, in particular, which are reported to be present in increased numbers in colorectal cancer cells as compared to normal tissue, could be characterised and localised. A quantitative approach was developed to assess the fraction of lipid bodies in the total cell area. This method was applied for comparison between malignant and non-malignant cell lines. The fraction of lipid bodies turned out to be significantly higher in malignant (13.8%, n = 21) than in non-malignant cells (1.8%, n = 16).

Conclusion

Confocal Raman microscopy is shown to be a powerful method for the investigation of lipid bodies.

Hintergrund und Zielsetzung

Die Untersuchung von lebenden Zellen unter physiologischen Bedingungen erfordert sensitive und nicht-invasive Methoden. Die Raman-Spektroskopie liefert über das Schwingungsverhalten von Molekülen Informationen über die chemische Komposition einer Probe. Die Kombination mit hochauflösender konfokaler Mikroskopie generiert ein komplettes Raman-Spektrum in jedem einzelnen konfokalen Bildpunkt. Diese Methode wurde angewendet um Lipid-Bodies in einer Kolonkarzinom-Zelllinie und in normalen Darmepithelzellen zu charakterisieren.

Materialien und Methoden

Die Adenokarzinom-Zelllinie Caco-2 und die Ratten-Darmepithel-Zelllinie IEC-6 wurden mit dem konfokalen Raman-Mikroskop alpha300 R (WITec GmbH, Ulm, Deutschland) unter Anregung durch einen Frequenz-verdoppelten Nd:YAG-

目的生理条件下活细胞的研究需要灵敏、精密,特别是非侵入性的方法。拉曼光谱提供了样品的振动信息。结合高分辨率共聚焦显微镜,它可以在每个共聚焦图像点记录完整的拉曼光谱。本研究将此技术应用于结肠癌细胞系脂质体的研究。材料与方法使用德国WITec公司alpha300 R共聚焦拉曼显微镜,采用532 nm、10 mW倍频Nd:YAG激光激发,对结直肠癌细胞系Caco-2和大鼠肠上皮细胞系IEC-6进行分析。使用水浸透镜(63×, NA 1.0),可实现亚微米范围内的横向分辨率。通过对特定拉曼波段进行积分,从数据集生成细胞的拉曼图像。C-H拉伸带(2800-3030 cm−1)的映射允许整个细胞的可视化,而通过k-means聚类分析对所有光谱的自动统计评估导致光谱未混合图像,从而提供了对样品化学成分的深入了解。结果采用该方法,可以直观地观察不同细胞生物标志物的分布。特别是脂质体,据报道,与正常组织相比,脂质体在结直肠癌细胞中的数量增加,可以被表征和定位。建立了一种定量方法来评估脂质体在总细胞面积中的比例。该方法用于恶性和非恶性细胞系的比较。恶性细胞中脂质体的比例(13.8%,n = 21)明显高于非恶性细胞(1.8%,n = 16)。结论共聚焦拉曼显微镜是研究脂质体的一种有效方法。研究进展与进展[j]。化学合成探测器与化学合成探测器。[0] [0] [0] [0] [0] [0] [0] [0] [0] [0]用Diese方法研究kolokarzinom - zelllinie和正常Darmepithelzellen的脂质体特征。材料与方法die adenokarzino - zelllinie caco2和die Ratten-Darmepithel-Zelllinie IEC-6 wurden mit dem konfokalen Raman-Mikroskop alpha300 R (WITec GmbH, Ulm, Deutschland) in Anregung durch einen frequency -verdoppelten Nd:YAG-Laser (532 nm, 10 mW)激光器。在63× wasserimmersion - object - mit数字孔径:1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0。Raman-Bilder von Zellen wurden durch Integration。模具集成 ber Die C-H-Streckschwingungsbande (2800-3030 cm-1) ergibt Bilder des gesamten Zellareals, während Die automatisierte statisticsche analyser Spektren mit k- means - clustering analysis Die verilung von Regionen mit ähnlicher chemischer Zusammensetzung wiedergibt。[3] [2] [1] [1] [1] [1] [1] [1] [1] [1] [2]脂质体的生物学性质,生物学性质,生物学性质,生物学性质,生物学性质和生物学性质。关于脂质体与gesamten关系的研究Zelloberfläche研究技术,定量研究。在恶性Zellen (13.8%, n = 21)和夜间肿瘤Zellen (1.8%, n = 16)中,Der Anteil和脂质体也有显著差异höher。引用本文:[j] .基于Raman-Mikroskopie的高效液相色谱法研究了kolonkarzinomzen中脂质体的研究进展。
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引用次数: 27
Concept of a fiber guidance instrument for laser-assisted laparoscopic partial nephrectomy 激光辅助腹腔镜部分肾切除术用光纤导引仪的概念
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.006
Wael Y. Khoder , Ronald Sroka

Objective

New developments in laser-assisted laparoscopic partial nephrectomy (LLPN) have created a demand for surgical tools which are compatible with laparoscopic equipment to ensure that laser-assisted technique is safer and reproducible. A concept was developed for a novel laser fiber guidance instrument based upon observations made during minimally invasive LLPN with a modified, commercially available ENT laser guidance instrument.

Patients and methods

Ten patients underwent laser-assisted laparoscopic/retroperitoneoscopic partial nephrectomy by means of fiber-assisted light application of 1318 nm using a surgical diode laser (Eraser; Rolle&Rolle, Austria). Observations made during the laparoscopic procedure were analyzed with a view to developing a novel laser fiber guidance instrument taking into account intraoperative laser-related requirements.

Results

All laparoscopic procedures were completed without any changeovers to open surgery. Tumor locations (kidney: 5 left/5 right) were central (1/10), upper pole (2/10), lower pole (2/10) and middle kidney parenchyma (5/10). The mean operation time and blood loss were comparable to standard techniques. All patients showed a favorable outcome with uneventful postoperative convalescence. A concept for a new instrument was developed taking into consideration the drawbacks associated with the clinical handling of the used modified ENT laser guidance instrument and ensuring that the new instrument is simple, cheap and easy to produce as well as easy to handle.

Conclusion

The in vivo observational study presented here shows that the 1318 nm emitting diode laser is a promising device for LLPN. Laser-based treatment facilitates for both good coagulation as well as cutting effects. The observations made resulted in a concept for an improved instrument. It is therefore conceivable that in the future there will be a wider use of LLPN for selective and precise laser resection of tumors with almost bloodless operation field and adequate fiber manipulation.

Zielsetzung

Neue Entwicklungen in der Laser-gestützten laparoskopischen partiellen Nephrektomie (LLPN) haben zu neuen Anforderungen an das chirurgische Instrumentarium geführt, um das Verfahren noch sicherer und reproduzierbarer zu machen. Basierend auf Handhabungsbeobachtungen während der minimal-invasiven LLPN unter Verwendung eines modifizierten HNO Lasersonden-Führungsinstrumentes wurde ein Konzept für eine neuartige Instrumentenvariante entwickelt.

Patienten und Methoden

10 Patienten wurden einer LLPN unterzogen, wobei ein 1318 nm Diodenlaser (Eraser; Rolle& Rolle, Österreich) zur Anwendung kam. Während der Eingriffe wurden Handhabungsbeobachtungen am verwendeten Lasersonden-Führungsinstrument durchgeführt, auf deren Basis die technischen Systemanforderungen analysi

目的激光辅助腹腔镜部分肾切除术(LLPN)的新发展对与腹腔镜设备兼容的手术工具产生了需求,以确保激光辅助技术的安全性和可重复性。基于在微创LLPN中使用改进的市售ENT激光制导仪进行的观察,开发了一种新型激光光纤制导仪的概念。患者和方法10例患者行激光辅助腹腔镜/后腹膜镜肾部分切除术,采用1318 nm光纤辅助光应用手术二极管激光器(Eraser;Rolle&罗尔、奥地利)。我们分析了腹腔镜手术过程中观察到的情况,并考虑到术中激光相关的要求,开发了一种新型激光光纤引导仪器。结果所有腹腔镜手术均顺利完成,未转开腹手术。肿瘤位置(肾:左5/右5)为中央(1/10)、上极(2/10)、下极(2/10)和中肾实质(5/10)。平均手术时间和出血量与标准技术相当。所有患者均表现出良好的预后,术后康复顺利。考虑到临床处理使用的改良耳鼻喉激光引导仪的缺点,开发了一种新仪器的概念,并确保新仪器简单,便宜,易于生产以及易于操作。结论1318nm发光二极管激光器是一种很有前途的LLPN器件。激光治疗有利于良好的凝血和切割效果。所作的观察产生了改进仪器的概念。因此可以想象,在未来LLPN将有更广泛的应用于选择性和精确的肿瘤激光切除,几乎无血的手术场和充分的纤维操作。激光成像成像技术在腹腔镜肾切除术(LLPN)中的应用研究(英文):1 .激光成像成像技术(英文)和激光成像技术(英文);微创LLPN的基础研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究:微创LLPN的研究;患者与方法:10例患者在1318 nm二极管激光擦除剂中使用LLPN不洁剂;Rolle&罗尔,Österreich) zur Anwendung kam。Während der Eingriffe wurden Handhabungsbeobachtungen am verwendeten lasersonden - fhng instrument durchgefhrt, auf deren Basis die technischen Systemanforderungen analyzer and inneuer instrumententwurf entwickelt wurden。ergebnisle Operationen konnten vollständig腹腔镜手术研究中心,dr .h. ergebnisle腹腔镜手术研究中心。5岁时,他的行动是在他和他的朋友之间进行的,5岁时,他和他的朋友在一起。Die tummoren selbst waren central (1/10), am nieerenoberpol (2/10), am nieerenunterpol(2/10)和am mittleren nieerenparenchym (5/10) lokalisiert。Die durchschnittliche Operationszeit and der auftretende Blutverlust waren der der Standardprozedur vergleichbar。所有患者均为阳性,并在黑龙江附近进行了检测。in Hinblick auf die beachteten Nachteile in der handhaung de verwendeten laser - of hundingsinstrumentenvariante entwickelt, under der ma ß ßgabe,在der der der Aufbau, preiswert and infacch in der production,在der Benutzung sein sollte。ZusammenfassungDie vorliegende in -体内machbarkees的研究weist den verwenddeten 1318 nm Diodenlaser als vielversprechendes Gerät frdie LLPN miter guten Koagulation and guten Schneideigenschaften aus。德国医学研究中心(Handhabungsbeobachtungen flossen),德国医学研究中心(verbesseres),德国医学研究中心(verbesseres),德国医学研究中心(verbesseres),德国医学研究中心(verbesseres)。Dies könnte zu einer breiteren Anwendbarkeit der LLPN f r die selictive und präzise肿瘤诊断和肿瘤诊断und Gewährleistung eines blutfreien Operationsfeldes und ausreichender Manipulationsmöglichkeiten f r die faster fhren。
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引用次数: 3
Protokoll der Mitgliederversammlung der Deutschen Gesellschaft für Lasermedizin (DGLM) e.V. 德国激光协会会员会议规程
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.007
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引用次数: 0
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.001
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引用次数: 0
Author and Subject Index 作者及主题索引
Pub Date : 2011-11-01 DOI: 10.1016/S1615-1615(11)00069-X
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引用次数: 0
Advertisement: Congress DGE-BV 广告:国会DGE-BV
Pub Date : 2011-11-01 DOI: 10.1016/j.mla.2011.08.010
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引用次数: 0
期刊
Medical laser application : international journal for laser treatment and research
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