Gallein is a known Gβγ subunit inhibitor, but its function in bone metabolism, especially in osteoblasts, and its molecular mechanism remains to be elucidated. Osteoprotegerin (OPG), which is secreted from osteoblasts, binds to nuclear factor kB receptor activator (RANK) ligand (RANKL) as a decoy receptor, prevents RANKL-RANK binding, and inhibits bone resorption. IL-6 is not only a bone resorption factor but also as a bone metabolism regulator. Prostaglandin F2α (PGF2α) promotes p44/p42 MAPK, p38 MAPK and stress-activated protein kinase/JNK phosphorylation in osteoblast-like MC3T3-E1 cells. In MC3T3-E1 cells, activated p44/p42 and p38 MAPKs promote IL-6 secretion and activated p44/p42 and p38 MAPKs and JNK promote OPG secretion. The present study aimed to investigate the effect and mechanism of gallein on PGF2α-induced OPG and IL-6 secretion using an osteoblastic MC3T3-E1 cell line. It was found that gallein significantly increased PGF2α-induced OPG and IL-6 secretion in the MC3T3-E1 cell. By contrast, fluorescein, which is a gallein-like compound that does not bind to Gβγ, did not affect PGF2α-induced OPG and IL-6 secretion. Gallein significantly improved the PGF2α-induced OPG and IL-6 mRNA expression levels. Gallein did not affect the PGF2α-activated phosphorylation of p44/p42 and p38 MAPKs and JNK. Gallein increased PGF2α-induced OPG and IL-6 secretion in osteoblasts, indicating that gallein may regulate bone remodeling via OPG/IL-6 in bone metabolism.
{"title":"Gallein increases prostaglandin F2α‑induced osteoprotegerin and IL‑6 secretion in osteoblasts.","authors":"Gen Kuroyanagi, Tomoyuki Hioki, Rie Matsushima-Nishiwaki, Takuya Omura, Osamu Kozawa, Haruhiko Tokuda","doi":"10.3892/br.2024.1835","DOIUrl":"10.3892/br.2024.1835","url":null,"abstract":"<p><p>Gallein is a known Gβγ subunit inhibitor, but its function in bone metabolism, especially in osteoblasts, and its molecular mechanism remains to be elucidated. Osteoprotegerin (OPG), which is secreted from osteoblasts, binds to nuclear factor kB receptor activator (RANK) ligand (RANKL) as a decoy receptor, prevents RANKL-RANK binding, and inhibits bone resorption. IL-6 is not only a bone resorption factor but also as a bone metabolism regulator. Prostaglandin F2α (PGF2α) promotes p44/p42 MAPK, p38 MAPK and stress-activated protein kinase/JNK phosphorylation in osteoblast-like MC3T3-E1 cells. In MC3T3-E1 cells, activated p44/p42 and p38 MAPKs promote IL-6 secretion and activated p44/p42 and p38 MAPKs and JNK promote OPG secretion. The present study aimed to investigate the effect and mechanism of gallein on PGF2α-induced OPG and IL-6 secretion using an osteoblastic MC3T3-E1 cell line. It was found that gallein significantly increased PGF2α-induced OPG and IL-6 secretion in the MC3T3-E1 cell. By contrast, fluorescein, which is a gallein-like compound that does not bind to Gβγ, did not affect PGF2α-induced OPG and IL-6 secretion. Gallein significantly improved the PGF2α-induced OPG and IL-6 mRNA expression levels. Gallein did not affect the PGF2α-activated phosphorylation of p44/p42 and p38 MAPKs and JNK. Gallein increased PGF2α-induced OPG and IL-6 secretion in osteoblasts, indicating that gallein may regulate bone remodeling via OPG/IL-6 in bone metabolism.</p>","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"21 5","pages":"147"},"PeriodicalIF":2.3,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asma' Al‑Zabin, Tuqa Abu Thiab, M. Zihlif, A. Al-Hunaiti, Hamzeh J. Al-Ameer, W. Al-Awaida, Amer Imraish
{"title":"Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe2ZnO4 nanoparticles against MCF‑7 cell line","authors":"Asma' Al‑Zabin, Tuqa Abu Thiab, M. Zihlif, A. Al-Hunaiti, Hamzeh J. Al-Ameer, W. Al-Awaida, Amer Imraish","doi":"10.3892/br.2024.1724","DOIUrl":"https://doi.org/10.3892/br.2024.1724","url":null,"abstract":"","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"5 17","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139439773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dominic-Gabriel Iliescu, R. Petrita, Cristina Teodorescu, R. Olaru, Andreea Alexa, Izabella Petre
{"title":"Real‑world performance and safety of vaginal ovules in reducing the vaginal symptoms associated with vulvovaginal atrophy and postmenopausal sexual dysfunction","authors":"Dominic-Gabriel Iliescu, R. Petrita, Cristina Teodorescu, R. Olaru, Andreea Alexa, Izabella Petre","doi":"10.3892/br.2024.1723","DOIUrl":"https://doi.org/10.3892/br.2024.1723","url":null,"abstract":"","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"32 34","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139442763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
George Fotakopoulos, Charalambos Gatos, V. Georgakopoulou, Ioannis G. Lempesis, Demetrios A. Spandidos, N. Trakas, P. Sklapani, Kostas Fountas
{"title":"Role of decompressive craniectomy in the management of acute ischemic stroke (Review)","authors":"George Fotakopoulos, Charalambos Gatos, V. Georgakopoulou, Ioannis G. Lempesis, Demetrios A. Spandidos, N. Trakas, P. Sklapani, Kostas Fountas","doi":"10.3892/br.2024.1721","DOIUrl":"https://doi.org/10.3892/br.2024.1721","url":null,"abstract":"","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"19 23","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139389430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Klaihmon, K. Pattanapanyasat, Phatchariya Phannasil
{"title":"An update on recent studies of extracellular vesicles and their role in hypercoagulability in thalassemia (Review)","authors":"P. Klaihmon, K. Pattanapanyasat, Phatchariya Phannasil","doi":"10.3892/br.2023.1719","DOIUrl":"https://doi.org/10.3892/br.2023.1719","url":null,"abstract":"","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"17 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139150371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenyun Kui, Lei Xie, Yanan Li, Zhen Gu, Shuang Liu, Chunchun Xue, Kaiqiang Wang
{"title":"Efficacy and safety of bloodletting puncture and cupping in postherpetic neuralgia: A systematic review and meta‑analysis","authors":"Wenyun Kui, Lei Xie, Yanan Li, Zhen Gu, Shuang Liu, Chunchun Xue, Kaiqiang Wang","doi":"10.3892/br.2023.1718","DOIUrl":"https://doi.org/10.3892/br.2023.1718","url":null,"abstract":"","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"68 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139150218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Infection with multi‑drug resistant organisms in patients with limb fractures: Analysis of risk factors and pathogens","authors":"Honggang Sun, Dagao Dong, Min Zhao, Jie Jian","doi":"10.3892/br.2023.1716","DOIUrl":"https://doi.org/10.3892/br.2023.1716","url":null,"abstract":"","PeriodicalId":8863,"journal":{"name":"Biomedical reports","volume":"59 30","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138945945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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