Pub Date : 2010-03-05DOI: 10.2174/1875043501003010001
Laura Ylä-Outinen, Christine Mariani, H. Skottman, R. Suuronen, A. Harlin, S. Narkilahti
{"title":"Electrospun Poly(L,D-lactide) Scaffolds Support the Growth of Human Embryonic Stem Cell-derived Neuronal Cells~!2009-08-26~!2009-11-30~!2010-02-12~!","authors":"Laura Ylä-Outinen, Christine Mariani, H. Skottman, R. Suuronen, A. Harlin, S. Narkilahti","doi":"10.2174/1875043501003010001","DOIUrl":"https://doi.org/10.2174/1875043501003010001","url":null,"abstract":"","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"361 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2010-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-04DOI: 10.2174/1875043500902010063
H. Shigematsu, M. Akahane, Y. Dohi, Akifumi Nakamura, H. Ohgushi, T. Imamura, Yasuhito Tanaka
We have developed a new cell transplantation technique, in which mesenchymal stem cells (MSCs) were cultured and lifted as a cell sheet structure. The sheet formed bone tissue after in vivo transplantation at a subcutaneous site, without the need for any scaffold. The sheet could also be transplanted with a scaffold, such as hydroxyapatite (HA). Transplantation of this sheet/HA construct resulted in extensive bone formation. The purpose of the present study was to analyze the spatial distribution and biological potential of the osteogenic sheet/HA constructs. Histological examination showed that the construct allowed bone formation inside the pores, as well as outside the HA at 2 weeks after transplantation. Interestingly, when two HAs were wrapped in one sheet, the bone on the outside of one HA could bond to the bone on the other HA, resulting in union of two HAs. Biochemical investigation showed that the sheet/HA constructs had higher osteogenic potential than previous methods, such as MSC/HA constructs. Our results indicate that this transplantation technique using MSC sheets has the potential to be a useful tool in bone tissue engineering.
{"title":"Osteogenic Potential and Histological Characteristics of Mesenchymal Stem Cell Sheet/Hydroxyapatite Constructs","authors":"H. Shigematsu, M. Akahane, Y. Dohi, Akifumi Nakamura, H. Ohgushi, T. Imamura, Yasuhito Tanaka","doi":"10.2174/1875043500902010063","DOIUrl":"https://doi.org/10.2174/1875043500902010063","url":null,"abstract":"We have developed a new cell transplantation technique, in which mesenchymal stem cells (MSCs) were cultured and lifted as a cell sheet structure. The sheet formed bone tissue after in vivo transplantation at a subcutaneous site, without the need for any scaffold. The sheet could also be transplanted with a scaffold, such as hydroxyapatite (HA). Transplantation of this sheet/HA construct resulted in extensive bone formation. The purpose of the present study was to analyze the spatial distribution and biological potential of the osteogenic sheet/HA constructs. Histological examination showed that the construct allowed bone formation inside the pores, as well as outside the HA at 2 weeks after transplantation. Interestingly, when two HAs were wrapped in one sheet, the bone on the outside of one HA could bond to the bone on the other HA, resulting in union of two HAs. Biochemical investigation showed that the sheet/HA constructs had higher osteogenic potential than previous methods, such as MSC/HA constructs. Our results indicate that this transplantation technique using MSC sheets has the potential to be a useful tool in bone tissue engineering.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"63-70"},"PeriodicalIF":0.0,"publicationDate":"2010-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-02DOI: 10.2174/1875043500902010053
Jennifer Dawson, Sophie Boisvenue, I. Skerjanc, M. Griffith
Our objective was to develop a suitable cardiomyocyte progenitor cell line for use in testing biomaterials as potential scaffolds in cardiac tissue engineering. We transfected P19 cells with the human cardiac -actin promoter driving the gene for puromycin resistance, to create a stable cardiomyocyte-selectable P19 cell line, termed P19(CA- Puro). Puromycin selection resulted in a 4-10 fold enrichment of cardiac muscle gene expression and a 3-fold enrichment in cardiomyocytes. Morphological, biochemical, and functional analyses were used to evaluate the properties of P19(CA- Puro) cardiomyocytes in the presence and absence of a novel cross-linked collagen-based biomaterial. The collagen-based biomaterial was able to support appropriate viability, gene expression, and cardiomyocyte function. Therefore, P19(CA- Puro) cells are suitable for examining biomaterials as potential scaffolds and this approach could be used for rapidly screening biomaterials for designing future human embryonic stem cell therapies.
{"title":"A P19 Cardiac Cell Line as a Model for Evaluating Cardiac Tissue Engineering Biomaterials","authors":"Jennifer Dawson, Sophie Boisvenue, I. Skerjanc, M. Griffith","doi":"10.2174/1875043500902010053","DOIUrl":"https://doi.org/10.2174/1875043500902010053","url":null,"abstract":"Our objective was to develop a suitable cardiomyocyte progenitor cell line for use in testing biomaterials as potential scaffolds in cardiac tissue engineering. We transfected P19 cells with the human cardiac -actin promoter driving the gene for puromycin resistance, to create a stable cardiomyocyte-selectable P19 cell line, termed P19(CA- Puro). Puromycin selection resulted in a 4-10 fold enrichment of cardiac muscle gene expression and a 3-fold enrichment in cardiomyocytes. Morphological, biochemical, and functional analyses were used to evaluate the properties of P19(CA- Puro) cardiomyocytes in the presence and absence of a novel cross-linked collagen-based biomaterial. The collagen-based biomaterial was able to support appropriate viability, gene expression, and cardiomyocyte function. Therefore, P19(CA- Puro) cells are suitable for examining biomaterials as potential scaffolds and this approach could be used for rapidly screening biomaterials for designing future human embryonic stem cell therapies.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"2009-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-11-10DOI: 10.2174/1875043500902010048
A. Barak, J. George, A. Lowenstein, M. Goldstein, Haim Shmlovich, A. Afek
Objective: Patients with age-related macular degeneration (AMD) exhibit pathologic neovascularization under the retina, with choroidal neovascularization (CNV) suggestive of defective angiogenesis. The endothelial progenitor cells (EPCs) present in the peripheral blood contribute to angiogenesis and vasculogenesis, and their regulation is altered in several vascular disorders. We investigated whether the numbers and functional properties of EPCs may be disordered in newly diagnosed neovascular AMD. Methods Fifteen suitable AMD patients and 10 controls matched for age, risk factors for atherosclerosis and use of medi- cation that could influence the circulating pool of EPCs were studied. Circulating EPCs were assayed by the col- ony-forming unit (CFU) method. The EPCs' adhesive capacity was studied by evaluating their ability to attach to fi- bronectin and cultured endothelial cells. Serum levels of vascular endothelial growth factor (VEGF) were studied and cor- related with EPC numbers. Results: The patients had significantly fewer circulating EPCs(16.5±2.8) compared to their controls (31±4.6; p=0.0085). The functional properties of both groups' EPCs were similar. Conclusions: The peripheral circulating pool of endothelial stem cells is altered in patients with newly diagnosed neovas- cular AMD, suggesting that pathologic angiogenesis may result from or influence the regulation of endothelial precursor circulation.
{"title":"Fewer Circulating Endothelial Progenitor Cells in Newly Diagnosed Neovascular AMD Patients","authors":"A. Barak, J. George, A. Lowenstein, M. Goldstein, Haim Shmlovich, A. Afek","doi":"10.2174/1875043500902010048","DOIUrl":"https://doi.org/10.2174/1875043500902010048","url":null,"abstract":"Objective: Patients with age-related macular degeneration (AMD) exhibit pathologic neovascularization under the retina, with choroidal neovascularization (CNV) suggestive of defective angiogenesis. The endothelial progenitor cells (EPCs) present in the peripheral blood contribute to angiogenesis and vasculogenesis, and their regulation is altered in several vascular disorders. We investigated whether the numbers and functional properties of EPCs may be disordered in newly diagnosed neovascular AMD. Methods Fifteen suitable AMD patients and 10 controls matched for age, risk factors for atherosclerosis and use of medi- cation that could influence the circulating pool of EPCs were studied. Circulating EPCs were assayed by the col- ony-forming unit (CFU) method. The EPCs' adhesive capacity was studied by evaluating their ability to attach to fi- bronectin and cultured endothelial cells. Serum levels of vascular endothelial growth factor (VEGF) were studied and cor- related with EPC numbers. Results: The patients had significantly fewer circulating EPCs(16.5±2.8) compared to their controls (31±4.6; p=0.0085). The functional properties of both groups' EPCs were similar. Conclusions: The peripheral circulating pool of endothelial stem cells is altered in patients with newly diagnosed neovas- cular AMD, suggesting that pathologic angiogenesis may result from or influence the regulation of endothelial precursor circulation.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"2009-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-07-28DOI: 10.2174/1875043500902010040
A. Larena, D. A. Cáceres, G. Ramos-Ortiz
Tissue engineering and regenerative medicine are disciplines in constant evolution to provide solutions, to assist and accelerate the regeneration and repairing of defective and damaged tissues. Many repair techniques produce inflammatory effects that difficult the regenerative process. In the present work we present a summary of our experience in using chitosan formulations as cells carrier for bone and cartilage regeneration. We have found that the use of chitosan lead to inflammatory processes in tissular repairing. To overcome this we proposed the introduction of anti-inflammatory products during the tissular regeneration and here we describe the experimental results on the control inflammatory effect in tissue engineering with chitosan nanoparticles under different sterilization process. It was found that chitosan nanoparticles have potential applications for several therapeutical drugs administration.
{"title":"Control Inflammatory Effect in Tissue Engineering with Chitosan Nanoparticles, Influence of Sterilization Process","authors":"A. Larena, D. A. Cáceres, G. Ramos-Ortiz","doi":"10.2174/1875043500902010040","DOIUrl":"https://doi.org/10.2174/1875043500902010040","url":null,"abstract":"Tissue engineering and regenerative medicine are disciplines in constant evolution to provide solutions, to assist and accelerate the regeneration and repairing of defective and damaged tissues. Many repair techniques produce inflammatory effects that difficult the regenerative process. In the present work we present a summary of our experience in using chitosan formulations as cells carrier for bone and cartilage regeneration. We have found that the use of chitosan lead to inflammatory processes in tissular repairing. To overcome this we proposed the introduction of anti-inflammatory products during the tissular regeneration and here we describe the experimental results on the control inflammatory effect in tissue engineering with chitosan nanoparticles under different sterilization process. It was found that chitosan nanoparticles have potential applications for several therapeutical drugs administration.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"40-47"},"PeriodicalIF":0.0,"publicationDate":"2009-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-08DOI: 10.2174/1875043500902010020
E. Monaco, A. Lima, M. Bionaz, A. Maki, S. Wilson, W. Hurley, M. Wheeler
In the present study we provided a morphological and transcriptomic comparison of adult porcine adipose- derived stem cells (ADSC) and bone marrow-derived stem cells (BMSC) as they differentiated in vitro towards the os- teogenic and adipogenic lineages for up to 4 weeks. The long term goal of this comparison is to assess the possibility of using ADSC as a potential alternative to BMSC as a source of autologous adult stem cells in human therapies. Our data indicated that ADSC can differentiate into osteocytes and adipocytes similar to BMSC but with some differ- ences. During the osteogenic differentiation both cell types went through morphological changes; however, while ADSC formed predominately osteogenic islands (nodules) in the culture dish, BMSC formed a continuous osteogenic sheet of small nodules. Transcriptomic analysis revealed that both cell types responded to the osteogenic induction. However, BGLAP mRNA expression did not increase in ADSC suggesting, together with the percentage area stained observed for Alizarin Red and von Kossa in ADSC, a lesser mineralization of bone matrix in this cell type compared to BMSC. During the adipogenic induction ADSC as well as BMSC were able to achieve the morphological and transcriptome changes characteristic of the adipogenic lineage. After 7 days of differentiation the expression patterns of DGAT2 and ADFP became greater in ADSC versus BMSC, which agreed with the larger lipid droplets formation observed in the ADSC by Oil Red O staining. Our findings represent an important step towards the assessment of using ADSC as an alternative to BMSC in therapeutic applications.
{"title":"Morphological and Transcriptomic Comparison of Adipose and Bone Marrow Derived Porcine Stem Cells","authors":"E. Monaco, A. Lima, M. Bionaz, A. Maki, S. Wilson, W. Hurley, M. Wheeler","doi":"10.2174/1875043500902010020","DOIUrl":"https://doi.org/10.2174/1875043500902010020","url":null,"abstract":"In the present study we provided a morphological and transcriptomic comparison of adult porcine adipose- derived stem cells (ADSC) and bone marrow-derived stem cells (BMSC) as they differentiated in vitro towards the os- teogenic and adipogenic lineages for up to 4 weeks. The long term goal of this comparison is to assess the possibility of using ADSC as a potential alternative to BMSC as a source of autologous adult stem cells in human therapies. Our data indicated that ADSC can differentiate into osteocytes and adipocytes similar to BMSC but with some differ- ences. During the osteogenic differentiation both cell types went through morphological changes; however, while ADSC formed predominately osteogenic islands (nodules) in the culture dish, BMSC formed a continuous osteogenic sheet of small nodules. Transcriptomic analysis revealed that both cell types responded to the osteogenic induction. However, BGLAP mRNA expression did not increase in ADSC suggesting, together with the percentage area stained observed for Alizarin Red and von Kossa in ADSC, a lesser mineralization of bone matrix in this cell type compared to BMSC. During the adipogenic induction ADSC as well as BMSC were able to achieve the morphological and transcriptome changes characteristic of the adipogenic lineage. After 7 days of differentiation the expression patterns of DGAT2 and ADFP became greater in ADSC versus BMSC, which agreed with the larger lipid droplets formation observed in the ADSC by Oil Red O staining. Our findings represent an important step towards the assessment of using ADSC as an alternative to BMSC in therapeutic applications.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"20-33"},"PeriodicalIF":0.0,"publicationDate":"2009-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-08DOI: 10.2174/1875043500902010034
F. Bucchieri, A. Fucarino, L. Rizzuto, A. Pitruzzella, A. Noto, F. Cappello, G. Zummo
Several acute and chronic inflammatory pathologies of the lung are accompanied by structural modifications of airway mucosa that vary depending on the severity, duration and type of the disease. These morphological changes, that determine organ dysfunction, are not always reversible. Indeed, the cycle of injury and repair, influencing airway wall re- generation, may sometimes break off and an exacerbation of the pathology may occur. The mechanisms at the base of airway remodelling during inflammation have been widely studied and numerous evidences indicate that the molecular dialogue among the cells of the mucosa has an essential role in orchestrating cell differentiation and tissue repair. In this review, we revise old notions on pulmonary morphology at the light of some of the most recent discoveries concerning stem cell differentiation, tissue homeostasis and organ regeneration of the lung.
{"title":"Stem Cell Populations and Regenerative Potential in Chronic Inflammatory Lung Diseases","authors":"F. Bucchieri, A. Fucarino, L. Rizzuto, A. Pitruzzella, A. Noto, F. Cappello, G. Zummo","doi":"10.2174/1875043500902010034","DOIUrl":"https://doi.org/10.2174/1875043500902010034","url":null,"abstract":"Several acute and chronic inflammatory pathologies of the lung are accompanied by structural modifications of airway mucosa that vary depending on the severity, duration and type of the disease. These morphological changes, that determine organ dysfunction, are not always reversible. Indeed, the cycle of injury and repair, influencing airway wall re- generation, may sometimes break off and an exacerbation of the pathology may occur. The mechanisms at the base of airway remodelling during inflammation have been widely studied and numerous evidences indicate that the molecular dialogue among the cells of the mucosa has an essential role in orchestrating cell differentiation and tissue repair. In this review, we revise old notions on pulmonary morphology at the light of some of the most recent discoveries concerning stem cell differentiation, tissue homeostasis and organ regeneration of the lung.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"34-39"},"PeriodicalIF":0.0,"publicationDate":"2009-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-07DOI: 10.2174/1875043500902010014
Valle Eva María Martin Del, P. HerreroEdgar, O. MartinsDinaA., A. GalánMiguel.
In this work a new system for immobilisation of cells was developed based on biocompatible alginate barium- films. Films are prepared to allow better control over film thickness and produce better film uniformity, avoiding rough- ness over film surface that can produce fibrosis and rejection after transplantation. In this study monocyte, mononuclear and fibroblast cell lines were immobilised in micrometric (� 300 microns of thick- ness) barium alginate films. The system was able to maintain the cell viability of all the three types of cells for at least two weeks after immobilisation, but cell proliferation was only verified for the monocyte and fibroblast cell lines.
{"title":"Immobilisation of Cells in Biocompatible Films to Cell Therapy","authors":"Valle Eva María Martin Del, P. HerreroEdgar, O. MartinsDinaA., A. GalánMiguel.","doi":"10.2174/1875043500902010014","DOIUrl":"https://doi.org/10.2174/1875043500902010014","url":null,"abstract":"In this work a new system for immobilisation of cells was developed based on biocompatible alginate barium- films. Films are prepared to allow better control over film thickness and produce better film uniformity, avoiding rough- ness over film surface that can produce fibrosis and rejection after transplantation. In this study monocyte, mononuclear and fibroblast cell lines were immobilised in micrometric (� 300 microns of thick- ness) barium alginate films. The system was able to maintain the cell viability of all the three types of cells for at least two weeks after immobilisation, but cell proliferation was only verified for the monocyte and fibroblast cell lines.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"14-19"},"PeriodicalIF":0.0,"publicationDate":"2009-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68109044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-21DOI: 10.2174/1875043500902010001
W. Krüger, T. Kiefer, C. Lotze, Fabian P. Thomsen, C. Hirt, F. Schüler, C. Busemann, G. Dölken
The chimeric monoclonal anti-CD20 antibody rituximab and the graft-versus-leukaemia/lymphoma effect after allogeneic stem cell transplantation have improved therapy of lymphatic malignancies during the last decade. In addition, successful therapy of chronic graft-versus-host disease after stem cell transplantation with rituximab has been published. Other investigators correlated prolonged neutropenia after autologous stem cell transplantation with prior rituximab- therapy. To address the question, if rituximab pre-treatment prior to transplantation diminishes the graft versus lymphoma effect afterwards and can prolong engraftment of allogeneic haemopoiesis, we retrospectively analysed data from 34 patients al- lografted for lymphoid malignancies. 19 patients had received at least one course of rituximab prior to allogeneic stem cell transplantation and 15 did not. Both groups matched very good. Patients with rituximab pre-treatment engrafted with 1 leukocyte/nl significantly faster than patients without prior rituxi- mab therapy (12 days (median, range 1-32) vs. 18 days (median, range 12-31), p<0,005). Engraftment of platelets (PLT) occurred as well faster after rituximab pre-treatment. The differences were significant for all three steps (20PLT/nl: 11,5 days (median, range 1-28) vs. 27 days (median, range 11-54) p<0,001; 50PLT/nl: 16,5 days (median, range 11-37) vs. 42 days (median, range 22-70) p<0,001; 100PLT/nl: 22,5 days (median, range 13-52) vs. 60 days (median, range 25-117) p<0,005). The mechanism leading to faster engraftment remains unclear so far. Preceding therapy with rituximab had no influence on event-free survival, overall survival, or manifestation freedom from graft-versus-host disease (GVHD) after allogeneic stem cell transplantation. Patients with GVHD had a significantly better overall survival (p=0,0001) comparing to patients without. In conclusion, this investigation gives no hint for a suppression of GvH and GvL effects by rituximab administration prior to allogeneic stem cell transplantation. In addition, in contrast to results published for autologous stem cell transplantation, no detrimental effects on establishment of graft haemopoiesis after allogeneic stem cell transplantation by preceding ri- tuximab therapy were found.
{"title":"Accelerated Allogeneic Haemopoietic Engraftment after Preceding Rituximab-Therapy","authors":"W. Krüger, T. Kiefer, C. Lotze, Fabian P. Thomsen, C. Hirt, F. Schüler, C. Busemann, G. Dölken","doi":"10.2174/1875043500902010001","DOIUrl":"https://doi.org/10.2174/1875043500902010001","url":null,"abstract":"The chimeric monoclonal anti-CD20 antibody rituximab and the graft-versus-leukaemia/lymphoma effect after allogeneic stem cell transplantation have improved therapy of lymphatic malignancies during the last decade. In addition, successful therapy of chronic graft-versus-host disease after stem cell transplantation with rituximab has been published. Other investigators correlated prolonged neutropenia after autologous stem cell transplantation with prior rituximab- therapy. To address the question, if rituximab pre-treatment prior to transplantation diminishes the graft versus lymphoma effect afterwards and can prolong engraftment of allogeneic haemopoiesis, we retrospectively analysed data from 34 patients al- lografted for lymphoid malignancies. 19 patients had received at least one course of rituximab prior to allogeneic stem cell transplantation and 15 did not. Both groups matched very good. Patients with rituximab pre-treatment engrafted with 1 leukocyte/nl significantly faster than patients without prior rituxi- mab therapy (12 days (median, range 1-32) vs. 18 days (median, range 12-31), p<0,005). Engraftment of platelets (PLT) occurred as well faster after rituximab pre-treatment. The differences were significant for all three steps (20PLT/nl: 11,5 days (median, range 1-28) vs. 27 days (median, range 11-54) p<0,001; 50PLT/nl: 16,5 days (median, range 11-37) vs. 42 days (median, range 22-70) p<0,001; 100PLT/nl: 22,5 days (median, range 13-52) vs. 60 days (median, range 25-117) p<0,005). The mechanism leading to faster engraftment remains unclear so far. Preceding therapy with rituximab had no influence on event-free survival, overall survival, or manifestation freedom from graft-versus-host disease (GVHD) after allogeneic stem cell transplantation. Patients with GVHD had a significantly better overall survival (p=0,0001) comparing to patients without. In conclusion, this investigation gives no hint for a suppression of GvH and GvL effects by rituximab administration prior to allogeneic stem cell transplantation. In addition, in contrast to results published for autologous stem cell transplantation, no detrimental effects on establishment of graft haemopoiesis after allogeneic stem cell transplantation by preceding ri- tuximab therapy were found.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2009-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68109034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.2174/1875043500902010008
K Zheng, M A Rupnick, B Liu, M E Brezinski
Optical Coherence Tomography (OCT) provides detailed, real-time information on the structure and composition of constructs used in tissue engineering. The focus of this work is the OCT three-dimensional assessment of scaffolding architecture and distribution of cells on it. PLGA scaffolds were imaged in two and three-dimensions, both seeded and unseeded with cells. Then two types of scaffolds were reconstructed in three dimensions. Both scaffolding types were examined at three different seeding densities. The importance of three-dimensional assessments was evident, particularly with respect to porosity and identification of asymmetrical cell distribution.
{"title":"Three Dimensional OCT in the Engineering of Tissue Constructs: A Potentially Powerful Tool for Assessing Optimal Scaffold Structure.","authors":"K Zheng, M A Rupnick, B Liu, M E Brezinski","doi":"10.2174/1875043500902010008","DOIUrl":"https://doi.org/10.2174/1875043500902010008","url":null,"abstract":"<p><p>Optical Coherence Tomography (OCT) provides detailed, real-time information on the structure and composition of constructs used in tissue engineering. The focus of this work is the OCT three-dimensional assessment of scaffolding architecture and distribution of cells on it. PLGA scaffolds were imaged in two and three-dimensions, both seeded and unseeded with cells. Then two types of scaffolds were reconstructed in three dimensions. Both scaffolding types were examined at three different seeding densities. The importance of three-dimensional assessments was evident, particularly with respect to porosity and identification of asymmetrical cell distribution.</p>","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"2 ","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789573/pdf/nihms89540.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28583484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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