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Comparison of Aflatoxin Yield from Peanut Isolate of Aspergillus flavus with Other Isolates 黄曲霉花生分离物与其他分离物黄曲霉毒素产量的比较
Pub Date : 2023-08-01 DOI: 10.18805/ajdfr.dr-2028
R. Sarmah, P. K. Subudhi, T. K. Dutta, P. Roychoudhury, J. Gali, Y. D. Singh
Background: Mouldy growth in food commodities by aflatoxin producing fungi is a serious health concern to animals as well as human beings due to the harmful effects of aflatoxins like hepatotoxicity, carcinogenicity, impairment of protein synthesis, formation of DNA adducts, etc. even in minute concentrations. A tropical climate with moderate temperature, high humidity and heavy rainfall provides optimal conditions for the growth of aflatoxin producing fungi like Aspergillus flavus in different food items. The current study was to examine if the substrate(s) in a food item has any role to play in aflatoxin production by A. flavus. Methods: The fungus was isolated from ten different edible materials and identified using standard procedure. The isolates were cultured in potato dextrose agar slants keeping the culture conditions identical in triplicates. The toxin extracted using chloroform as solvent and downstream processing to get crude toxin was quantified on the basis of dry weight. Thin layer chromatography was done and the fluorescence of the toxins from different isolates was visualised under high wavelength of ultra-violet light with standard Aflatoxin B1 chromatographed vis-a-vis to confirm presence of the toxin in all the extracted toxins. Result: Results of the extracted toxin dry weight comparison between different isolates as well as the fluorescence intensity in the chromatograms revealed that contaminated peanut isolate of A. flavus yielded the highest amount of crude toxin. Thus, the results indicate that there are some factor(s) in peanut that promote(s) aflatoxin production by A. flavus. This information was further used for formulating potato dextrose agar media for isolation of aflatoxin producing fungi by incorporating peanut extract in the media and comparing the amount of crude toxin yield.
背景:产生黄曲霉毒素的真菌在食品中滋生霉菌,对动物和人类都是一个严重的健康问题,因为黄曲霉毒素具有肝毒性、致癌性、蛋白质合成受损、DNA加合物形成等有害影响,即使在很小的浓度下也是如此。温和温度、高湿度和强降雨的热带气候为黄曲霉等产生黄曲霉毒素的真菌在不同食品中的生长提供了最佳条件。目前的研究是检查食品中的底物是否在黄曲霉产生黄曲霉毒素中发挥任何作用。方法:从10种不同的食用材料中分离得到该真菌,并采用标准程序进行鉴定。分离株在马铃薯葡萄糖琼脂斜面中培养,保持培养条件相同,一式三份。以三氯甲烷为溶剂提取毒素,经下游加工得到粗毒素,以干重为基础进行定量。进行薄层色谱,并在高波长紫外线下观察来自不同分离物的毒素的荧光,用标准黄曲霉毒素B1进行色谱分析,以确认所有提取的毒素中都存在毒素。结果:不同分离物的提取毒素干重比较以及色谱图中的荧光强度结果表明,受污染的花生黄曲霉分离物产生的粗毒素量最高。因此,研究结果表明,花生中存在一些促进黄曲霉产生黄曲霉毒素的因素。该信息进一步用于配制马铃薯葡萄糖琼脂培养基,通过在培养基中加入花生提取物并比较粗毒素产量来分离黄曲霉毒素产生真菌。
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引用次数: 0
Effects of Foxtail Millet Flour (Setaria italica) on the Proximate Composition, Texture Profile, Colour Profile and Microbiological Qualities of Duck Meat Sausages 谷子粉对鸭肉香肠近似成分、质构、色泽及微生物品质的影响
Pub Date : 2023-08-01 DOI: 10.18805/ajdfr.dr-2094
K. Handique, S. Laskar, M. Raquib, S. Choudhury, S. Upadhyay, D. Bora, S. Tamuly
Background: Dietary fiber can be effectively incorporated into processed meat products along with binders, extenders and fillers. Using millet in meat products can balance the enormous demand and supply for affordable, wholesome, dietary fiber enriched and healthful meat products. Methods: The duck meat sausages were developed by incorporating three different levels of roasted foxtail millet flour (FTMF) i.e. 5%, 10% and 15% and coded as T1(5%), T2(10%) and T3 (15%). The proximate composition, texture profile, colour profile and microbiological qualities of the control and treated sausages were estimated. Result: The moisture, crude protein, ether extract and calorie value of the sausages declined significantly (p less than 0.05) with increasing levels of FTMF in the treated sausages. In the case of texture profile, a significant (p less than 0.05) difference was observed in the springiness, hardness, chewiness and resilience (p less than 0.05) of the sausages between the control and treated groups. However, no significant difference in cohesiveness was observed between the control and the treated products. The colour profile revealed no significant (p greater than 0.01) difference in lightness (L*), redness (a*) and yellowness (b*). TVC and TVPBC showed a significantly (p less than 0.01) decreasing trend from control to treated products. However, the bacterial load increased during the storage for up to 15 days. No colititre, yeast, or mould were detected during the entire storage period.
背景:膳食纤维可以作为粘合剂、填充剂和填充剂有效地加入加工肉制品中。在肉制品中使用小米可以平衡对价格合理、健康、富含膳食纤维的健康肉制品的巨大需求和供应。方法:采用5%、10%和15%三种不同水平的烤尾小米粉(FTMF),分别编码为T1(5%)、T2(10%)和T3(15%)的鸭肉香肠。对对照和处理过的香肠的近似组成、质地、颜色和微生物质量进行了估计。结果:随着FTMF含量的增加,香肠的水分、粗蛋白质、粗脂肪和热量值显著下降(p < 0.05)。在质构方面,对照组和处理组香肠的弹性、硬度、嚼劲和回弹性差异显著(p < 0.05)。然而,在对照组和处理过的产品之间没有观察到明显的凝聚力差异。颜色分布显示明度(L*)、红度(a*)和黄度(b*)无显著差异(p > 0.01)。TVC和TVPBC呈极显著(p < 0.01)下降趋势。然而,在长达15天的储存期间,细菌负荷增加。在整个贮存期间,未检测到酵母菌、酵母菌或霉菌。
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引用次数: 0
Formulation, Characterization and Storage Stability of Prickly Pear (Opuntia ficus) Fruit and Milk Based Fermented Beverage 仙人掌果乳发酵饮料的配方、特性及贮存稳定性
Pub Date : 2023-08-01 DOI: 10.18805/ajdfr.dr-2113
Nikunj M. Vachhani, K. Ahuja, A. J. Thesiya, Tanmay Hazra, V. Ramani
Background: Fermented milk based beverages are popular all over the world and cherished by people of all ages. Health conscious consumers nowadays desire for beverages with enhanced functionality. Prickly pear fruit crop having resilience to grow in arid and semi-arid lands, possess excellent antioxidant and natural food colorant potential. Present investigation was an attempt to combine prickly pear fruit with fermented milk. Methods: Effect of milk fat, pulp level and sugar replacement with low calorie sweetener was investigated on the sensory acceptability. Optimized prickly pear fruit and milk based beverages was evaluated for nutritional, colour, antioxidant properties and consumer acceptance. Stability of pear fruit and milk based beverage under refrigeration storage was analyzed. Result: Consumer acceptability test (n=100) of selected formulation indicated wide acceptability with overall liking score of 8.17±0.05 on a 9-point hedonic scale. Antioxidant potential of optimized prickly pear fruit and milk based beverage was significantly higher (648±6.11 TEAC/g) than the control samples (410.33±5.49 TEAC/g). Packed beverage in glass bottles remained acceptable up to 9th day.
背景:发酵乳饮料在世界各地都很受欢迎,受到各个年龄段人们的珍视。如今,注重健康的消费者渴望功能增强的饮料。刺梨果实作物具有在干旱和半干旱地区生长的韧性,具有良好的抗氧化剂和天然食品着色剂潜力。目前的研究是尝试将刺梨果实与发酵乳结合。方法:研究低热量甜味剂替代乳脂肪、果肉水平和糖含量对感官可接受性的影响。对优化的刺梨果和牛奶饮料的营养、颜色、抗氧化性能和消费者接受度进行了评估。分析了梨乳饮料在冷藏条件下的稳定性。结果:所选配方的消费者可接受性测试(n=100)显示出广泛的可接受性,在9分特征量表上的总体喜好得分为8.17±0.05。优化后的刺梨果乳基饮料的抗氧化能力(648±6.11 TEAC/g)显著高于对照样品(410.33±5.49 TEAC/g)。装在玻璃瓶中的饮料直到第9天仍然可以接受。
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引用次数: 0
Utilization of the Whole Cowpea Pod and Barley Husk in The Production of Nutritionally Enriched Composite Flour 全豇豆荚和大麦壳在营养强化复合粉生产中的应用
Pub Date : 2023-07-31 DOI: 10.18805/ajdfr.dr-1986
Urvashi, Anuradha Dutta, R. Raghuvanshi, Y. Singh, Nivedita, S. Tilara, D. Joshi
Background: Cowpea is a climbing annual crop from Fabaceae family which is grown for its edible seeds and pods. Cowpea is rich in various nutrients such as fibre, protein, iron, potassium and is low in fat and calories. It has been observed that non-Communicable diseases are increasing at a rapid rate in India as well as globally. The need of the hour is to control the rate of diseases through modification in dietary practices. This study has focused on formulation of whole cowpea pod enriched composite flour by including more fibre and various nutrients in the diet. Methods: In the study, composite flour using whole cowpea pod flour, barley husk flour and whole wheat flour was developed. The nutritional characteristics of composite flour and barley husk were analyzed. Storage study with two different packaging materials was also done. Result: The composite flour was found to have good nutritional properties as it contained valuable amount of protein, energy and crude fibre. It was also found that the flour had higher content of iron, magnesium and calcium while barley husk had higher content of manganese. Laminated aluminium pouches found to be more suitable for use as a packaging material.
背景:豇豆是豆科一年生攀缘作物,其种子和豆荚可食用。豇豆富含多种营养物质,如纤维、蛋白质、铁、钾,脂肪和卡路里含量低。据观察,非传染性疾病在印度和全球都在迅速增加。当务之急是通过改变饮食习惯来控制疾病的发病率。本研究的重点是通过在膳食中添加更多的纤维和各种营养成分来制备全豇豆豆荚复合面粉。方法:采用全豇豆豆荚粉、大麦壳粉和全麦粉配制复合面粉。分析了复合面粉和大麦皮的营养特性。并进行了两种不同包装材料的贮藏研究。结果:该复合面粉含有大量的蛋白质、能量和粗纤维,具有良好的营养特性。面粉中铁、镁、钙含量较高,大麦皮中锰含量较高。叠铝袋被发现更适合用作包装材料。
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引用次数: 0
Softened Water and Steam Boiler Water Condensate Quality used in the Milk Production Line of a Private Dairy Unit Located in Bechar Province (South-West of Algeria): Physicochemical and Bacteriological Analysis 位于阿尔及利亚西南部贝查尔省的一家私营乳品厂牛奶生产线上使用的软化水和蒸汽锅炉冷凝水水质:理化和细菌学分析
Pub Date : 2023-07-31 DOI: 10.18805/ajdfr.drf-327
E. Benyagoub
Background: This study aimed at assessing the physicochemical and bacteriological parameters of the water at the softener outlet and the steam boiler water condensate of a dairy unit located in Bechar province (South-West of Algeria). Methods: Some physicochemical and bacteriological parameters were analyzed based on standard methods given by the American Public Health Association (APHA). Result: The obtained results showed that the parameters: pH, EC, salinity, TA, TAC, sulfate, nitrate, and fluoride ions of most analyzed water samples at the softener outlet (SW) were in agreement with national regulations. However, the steam boiler water condensate samples (BW) were out of specification except for TA, TAC, nitrate, and fluoride ions. The SW and BW samples were qualified as very hard exceeding the standard set at 15°f and 10,5 to 11,5°f, respectively, with high salinity and TDS values. The bacteriological analysis showed that most of the steam boiler water condensate samples complied with national regulations with the absence of fecal coliforms, knowing that the BW5 and BW8 samples were contaminated with spores of sulfite-reducing Clostridia at a load of 3 and 1 spore/50 mL, respectively. However, an average of 6/10 (i.e. 60%) of the softened water samples were contaminated with the opportunistic species ‘Pseudomonas aeruginosa’, while the SW5, SW8 and SW10 samples were contaminated with spores of sulfite-reducing Clostridia (1 to 11 spores/50 mL). All samples were free from Salmonella spp. A high TH of the water at the softener outlet reveals a malfunction of the external treatment (softening). On the one hand, boiler scaling that may occur can reduce the steam flow (low efficiency of the heat treatment), which contributes to poor pasteurization of the milk, especially in the presence of bacterial contaminants, and on the other hand, increase the energy expenditure.
背景:本研究旨在评估贝查尔省(阿尔及利亚西南部)一家乳品厂软化剂出口处的水和蒸汽锅炉冷凝水的物理化学和细菌学参数。方法:根据美国公共卫生协会(APHA)给出的标准方法,对一些理化和细菌学参数进行分析。结果:软化器出水水质的pH、EC、盐度、TA、TAC、硫酸盐、硝酸盐、氟离子等参数符合国家规定。然而,除TA、TAC、硝酸盐和氟离子外,蒸汽锅炉冷凝水样品(BW)不符合规范。SW和BW样品分别在15°f和10,5至11,5°f时被评定为非常硬,超过标准,盐度和TDS值较高。细菌分析表明,大多数蒸汽锅炉冷凝水样本符合国家规定,不存在粪便大肠菌群,因为已知BW5和BW8样本分别被硫酸盐还原梭菌孢子污染,负荷分别为3和1个孢子/50 mL。然而,平均6/10(即60%)的软化水样品被机会菌种“铜绿假单胞菌”污染,而SW5、SW8和SW10样品被还原亚硫酸盐的梭状芽孢杆菌孢子污染(1-11个孢子/50mL)。所有样品均不含沙门氏菌。软化器出口处的水TH较高,表明外部处理(软化)出现故障。一方面,可能发生的锅炉结垢会减少蒸汽流量(热处理效率低),这会导致牛奶的巴氏灭菌效果不佳,尤其是在存在细菌污染物的情况下,另一方面还会增加能源消耗。
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引用次数: 0
Forage Production in India: Bottlenecks and Opportunities by Opening up New Way with an Increase in Fodder Oats Demand: A Review 印度饲料生产:随着饲料燕麦需求的增加,开辟新途径带来的瓶颈和机遇:综述
Pub Date : 2023-07-28 DOI: 10.18805/ajdfr.dr-2050
Harshavardan J. Hilli, R. Kapoor, A. -
In India, mixed farming and cattle raising is an essential elements of rural life and these practices are connected with the social structure in cultural, religious and economic ways. India’s production of fodder is very unevenly distributed and how this resource is used depends on the breed of cattle raised, the climate, the socioeconomic context and the crop-growing patterns. Fodder from cultivated regions is regularly fed to cattle and buffalo, with some harvested grasses and top feeds added as supplements. On the other hand within the land area of just 2.3 per cent, India is home to 16.8 per cent of the world’s population and nearly 20 per cent of the world’s cattle. India homes cattle (16%), buffalo (55%), goats (20%) and sheep (5%) of worlds total livestock population respectively. This fact makes it more cumbersome to meet the forage demand by livestock, thus shift in paradigm and opportunity in near future is about to hike for fodder crops with their potentiality in production per unit area. The present Indian scenario of fodder crops in comparison with the livestock population is being described in this article along with the net deficit and surplus availability of green and dry fodder. Further, this review also includes the opening of a new way for oats to contribute to fodder production. The challenges facing India for fodder production and its near opportunity to open up and evolve with various methodologies to tackle the present fodder demand by enhancing the production is described in detail in an article.
在印度,混合耕种和养牛是农村生活的重要组成部分,这些做法在文化、宗教和经济方面与社会结构有关。印度的饲料生产分布非常不均衡,如何使用这种资源取决于饲养的牛的品种、气候、社会经济环境和作物生长模式。种植区的饲料定期喂养牛和水牛,并添加一些收获的草和顶级饲料作为补充。另一方面,在仅有2.3%的土地面积内,印度拥有世界16.8%的人口和近20%的牲畜。印度饲养的牛(16%)、水牛(55%)、山羊(20%)和绵羊(5%)分别占世界牲畜总数。这一事实使满足牲畜的饲料需求变得更加困难,因此,在不久的将来,饲料作物的单位面积生产潜力将增加其模式和机会的转变。本文描述了目前印度饲料作物与牲畜数量的比较情况,以及绿色和干饲料的净短缺和过剩情况。此外,这篇综述还包括开辟燕麦为饲料生产做出贡献的新途径。一篇文章详细描述了印度饲料生产面临的挑战,以及通过提高产量来解决当前饲料需求的各种方法的开放和发展机会。
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引用次数: 0
Comparative Evaluation of Manual and Mechanical Jamun Fruit Harvesting 贾门果人工与机械采收的比较评价
Pub Date : 2023-07-27 DOI: 10.18805/ajdfr.dr-2107
I. Sahu, M. Panda, U. S. Pal, M. Mohapatra, G. Acharya
Background: An engine operated jamun harvester to shake the limb with adjustable shaking frequency and amplitude was developed. The aim of the present study was to evaluate the performance of the jamun harvester and determine the suitable shaking frequency and amplitude to obtain maximum harvesting efficiency. Methods: A 4×3 factorial experiment with completely randomized design was conducted to investigate the effects of shaking amplitude (10, 20, 30 and 40 mm) and frequency (2, 4 and 6 Hz) on percentage matured jamun harvested, fruit removal percentage and harvesting efficiency. Result: Analysis of variance and mean comparison showed that the shaking amplitude and shaking frequency both had significant effect on percentage matured jamun harvested, fruit removal percentage and harvesting efficiency. The fruit removal percentage increased at higher levels of shaking amplitude and frequency. Complete fruit removal (100%) was achieved at 6 Hz shaking frequency and 40 mm amplitude, however shattering of leaves and immature fruits were observed. Therefore, shaking amplitude of 30 mm and shaking frequency of 6 Hz was recommended for Jamun harvesting with 93.15% fruit removal and 64.33% harvesting efficiency. The fruit detachment force to fruit weight ratio (FDF/W) of Jamun varied from 1.46 to 0.23. The cost of harvesting of jamun by the developed harvester was found to be approx. Rs. 9/- as compared to Rs. 15/- per kg fruit for manual harvesting.
背景:研制了一种振动频率和振幅可调的发动机驱动的机械臂振动装置。本研究的目的是评估jamun收割机的性能,确定合适的振动频率和振幅,以获得最大的收获效率。方法:采用完全随机设计的4×3因子试验,研究振动幅度(10、20、30和40 mm)和频率(2、4和6 Hz)对jamun成熟采收率、去果率和采收效率的影响。结果:方差分析和均值比较表明,摇幅和摇频对熟果采收率、去果率和采收效率均有显著影响。振动幅度和频率越高,果实脱粒率越高。在6 Hz的振动频率和40 mm的振幅下,果实被完全去除(100%),但观察到叶片和未成熟果实破碎。因此,建议振幅为30 mm,振频为6 Hz,可获得93.15%的果去除率和64.33%的收获效率。Jamun果实分离力与果实重比(FDF/W)变化范围为1.46 ~ 0.23。开发的采收机采收jamun的成本约为。每公斤水果9卢比,而手工采摘每公斤水果15卢比。
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引用次数: 0
Development of Egg Nuggets with Addition of Pearl Millet 添加珍珠小米的鸡蛋块的研制
Pub Date : 2023-07-27 DOI: 10.18805/ajdfr.dr-2038
G. ., Kuntal Das, S. Verma, A. Patyal, C. Sahu
Background: A study was conducted to develop egg nugget incorporated with pearl millet to improve its acceptability and nutritional value. Eggs being an ideal diet for humans for its high nutritional properties that also fulfils special dietary requirements. Egg nuggets are the novel convenience egg products to be developed to fulfil a number of objectives including preparation of delicious ready to eat egg products with positive health effects. The beneficial effect of egg may be improved with addition of fibre in the nugget with a greater acceptance among the consumers. Methods: The egg nuggets were developed by using pearl millet flour at three different levels viz. T1(21.5%), T2 (19.5%) and T3 (17.5%). 19.5% level of pearl millet flour was selected on the basis of sensory attributes. Result: The variant T2 (19.5% Pearl Millet) was awarded highest overall palatability score Flavour, juiciness and texture scores were also found to be significantly (p less than 0.05) higher. Therefore, from the present study it was concluded that egg nuggets with 19.5% pearl millet flour had a better sensory quality and acceptability.
背景:为了提高珍珠小米的可接受性和营养价值,研制了珍珠小米蛋块。鸡蛋是人类的理想饮食,因为它具有高营养特性,也满足特殊的饮食要求。蛋块是一种新型方便蛋制品,旨在实现多项目标,包括制备具有积极健康效果的美味即食蛋制品。鸡蛋的有益效果可以通过在鸡块中添加纤维来改善,消费者更容易接受。方法:采用T1(21.5%)、T2(19.5%)和T3(17.5%)三个不同水平的珍珠小米粉制作鸡块,根据感官性状选择19.5%水平的珍珠米粉。结果:变体T2(19.5%珍珠小米)的总体适口性得分最高。风味、多汁性和质地得分也显著高于(p<0.05)。因此,本研究得出结论,添加19.5%珍珠小米粉的蛋块具有更好的感官质量和可接受性。
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引用次数: 0
Antibiotic Resistance Pattern and Distribution of Resistance Genes in Salmonella Isolated from Chicken and Duck Eggs in Chhattisgarh, India 印度恰蒂斯加尔邦鸡鸭蛋中沙门氏菌的耐药模式及耐药基因分布
Pub Date : 2023-07-25 DOI: 10.18805/ajdfr.dr-2033
T. Ganjeer, A. Patyal, S. Shakya, C. Chandrakar, S. Verma, N. Gade, C. Sannat, V. Naik, S. Parkar
Background: Salmonella is recognized as the most prevalent bacterial cause of foodborne diseases worldwide and animal-sourced foods have been reported as a common source of Salmonella infections among humans. Methods: The commercial chicken eggs, backyard chicken eggs, and duck eggs samples, 60 each, were processed for isolation and identification of Salmonella. All Salmonella isolates were further tested for resistance against six different antibiotics. The prevalence of virulence and antimicrobial resistance genes in the Salmonella isolates was determined by PCR. Result: A total of 28 Salmonella isolates were recovered with an overall prevalence of 15.6% and out of them, 11.1% and 4.4% were from eggshell and egg content, respectively. All the isolates were found sensitive to Gentamicin however maximum resistance was observed against Cefotaxime. PCR results revealed that 100% of the isolates were carrying the invA gene however stn gene was detected in 78.6% of isolates. Among presumptively identified β-lactam-resistant Salmonella isolates, 100% and 50% isolates harbored blaTEM and blaCTX-M genes, respectively whereas none of the isolates contained the blaSHV gene. All tetracycline-resistant isolates harbored the tetA gene whereas none of the isolates carried the tetB gene. 100% of fluoroquinolone-resistant isolates were carrying the gyrA gene however parC gene was present only in 60% of isolates. These results indicate that drug-resistant Salmonella spp. were prevalent in eggs sold in the study area which can pose a serious public health problem.
背景:沙门氏菌被认为是世界范围内食源性疾病最常见的细菌原因,据报道,动物源性食品是人类感染沙门氏菌的常见来源。方法:对商品鸡蛋、农家鸡蛋、鸭蛋各60个样品进行沙门氏菌的分离鉴定。所有沙门氏菌分离株都被进一步测试对六种不同抗生素的耐药性。通过聚合酶链式反应测定沙门氏菌分离株中毒力和抗微生物耐药性基因的流行率。结果:共回收28株沙门氏菌,总检出率为15.6%,其中蛋壳和鸡蛋分别占11.1%和4.4%。所有分离株均对庆大霉素敏感,但对头孢噻肟的耐药性最高。PCR结果显示,100%的分离株携带invA基因,78.6%的分离株检测到stn基因。在推定鉴定的β-内酰胺耐药沙门氏菌分离株中,100%和50%的分离株分别携带blaTEM和blaCTX-M基因,而没有一个分离株含有blaSHV基因。所有四环素抗性菌株都携带tetA基因,而没有一个菌株携带tetB基因。100%的氟喹诺酮耐药分离株携带gyrA基因,而parC基因仅存在于60%的分离株中。这些结果表明,在研究区域出售的鸡蛋中普遍存在抗药性沙门氏菌,这可能会造成严重的公共卫生问题。
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引用次数: 0
Formulation and Numerical Optimization of Finger Millet and Quinoa Incorporated Eggless Doughnuts 小米加藜麦无蛋甜甜圈的配方及数值优化
Pub Date : 2023-07-22 DOI: 10.18805/ajdfr.dr-2072
Ayushi Joshi, S. Srivastava, N. Shahi, A. Kushwaha, R. Acharya
Background: The consumption of finger millet and quinoa can be increased by partially substituting refined wheat flour (RWF) with these grains in popular snack food item doughnuts. The present study was conducted to formulate eggless doughnuts using composite flour of RWF, finger millet flour and quinoa flour. Method: Response Surface Methodology was used for optimization of process parameters for which seventeen different combinations of finger millet flour: quinoa flour (X1), butter (X2) and curd (X3) were taken as independent variables. The effect of independent variables on the responses (crude protein, crude fat, in vitro protein digestibility, springiness, flavor, appearance) was investigated. Result: Optimized doughnuts (X1=3:1, X2=16.712% of composite flour and X3=25% of composite flour) were formulated, analyzed and compared with control RWF doughnuts prepared under similar conditions for nutritional quality, hardness and sensory quality. Significantly higher values were observed for total ash, crude protein, crude fibre, hardness and springiness of optimized doughnuts in comparison to control RWF doughnuts whereas sensory evaluation showed non-significant difference except for color of the doughnuts. This study showed that the formulated eggless doughnuts were nutritious and could be a good substitute for the market based doughnuts especially for vegetarian people and who are allergic to egg protein.
背景:在流行的休闲食品甜甜圈中,用精制小麦粉(RWF)部分代替小米和藜麦,可以增加小米和藜麦的消费量。本研究采用RWF粉、小米粉和藜麦粉复合粉制作无蛋甜甜圈。方法:以藜麦粉(X1)、黄油(X2)、凝乳(X3)等17种不同组合为自变量,采用响应面法进行工艺参数优化。考察了自变量(粗蛋白质、粗脂肪、体外蛋白质消化率、弹性、风味、外观)对反应的影响。结果:配制出优化后的甜甜圈(X1=3:1, X2=16.712%复合面粉,X3=25%复合面粉),并与相同条件下制备的对照RWF甜甜圈进行营养品质、硬度和感官品质的分析比较。优化后的甜甜圈在总灰分、粗蛋白质、粗纤维、硬度和弹性等指标上均显著高于对照,但在感官评价方面,除了甜甜圈的颜色外,差异不显著。这项研究表明,配方的无蛋甜甜圈营养丰富,可以很好地替代市面上的甜甜圈,特别是对于素食者和对鸡蛋蛋白过敏的人。
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Journal of dairying, foods & home sciences
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