Pub Date : 2020-01-01DOI: 10.1016/b978-0-12-816053-4.09989-2
{"title":"Acknowledgments","authors":"","doi":"10.1016/b978-0-12-816053-4.09989-2","DOIUrl":"https://doi.org/10.1016/b978-0-12-816053-4.09989-2","url":null,"abstract":"","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88031598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.1016/b978-0-12-816053-4.09992-2
Divya J Aickara, R. Alessandro, S. Alipoor, R. Andriantsitohaina, Jun Araya, Koji Asano, Evangelos V. Badiavas, M. Bailey, Scott E Bonner, Han Chen, Yong Cheng, Raul Coimbra, A. Conigliaro, Denis Corbeil, C. Corrado, Todd W. Costantini, D. Crețoiu, S. Crețoiu, A. Cronemberger-Andrade, J. Dear, A. Deftu, A. Deftu, Shinichiro Egawa, B. Eliceiri, S. Fontana, Y. Fujita, Andrew F. Hill, Robert W. Hunter, T. Kadota, Ju‐Seop Kang, N. Kosaka, K. Kuwano, S. Le Lay, A. Lorico, Imre Mäger, M. C. Martinez, J. McBride, E. Mortaz, Soumyalekshmi Nair, Denis Noble, Takahiro Ochiya, S. Pang, B. Radu, Stefania Raimondo, L. Saieva, C. Salomón, Jeff S. Schorey, J. Sundstrom, Sin-Yeang Teow, A. Torrecilhas, F. Urabe, Sarah Weber, Eduard Willms, M. Wood, P. Xander, Junjie Xiao, Zhongdang Xiao, Yuanjun Zhao, Mi Zhou, Yueyuan Zhou
{"title":"Contributors","authors":"Divya J Aickara, R. Alessandro, S. Alipoor, R. Andriantsitohaina, Jun Araya, Koji Asano, Evangelos V. Badiavas, M. Bailey, Scott E Bonner, Han Chen, Yong Cheng, Raul Coimbra, A. Conigliaro, Denis Corbeil, C. Corrado, Todd W. Costantini, D. Crețoiu, S. Crețoiu, A. Cronemberger-Andrade, J. Dear, A. Deftu, A. Deftu, Shinichiro Egawa, B. Eliceiri, S. Fontana, Y. Fujita, Andrew F. Hill, Robert W. Hunter, T. Kadota, Ju‐Seop Kang, N. Kosaka, K. Kuwano, S. Le Lay, A. Lorico, Imre Mäger, M. C. Martinez, J. McBride, E. Mortaz, Soumyalekshmi Nair, Denis Noble, Takahiro Ochiya, S. Pang, B. Radu, Stefania Raimondo, L. Saieva, C. Salomón, Jeff S. Schorey, J. Sundstrom, Sin-Yeang Teow, A. Torrecilhas, F. Urabe, Sarah Weber, Eduard Willms, M. Wood, P. Xander, Junjie Xiao, Zhongdang Xiao, Yuanjun Zhao, Mi Zhou, Yueyuan Zhou","doi":"10.1016/b978-0-12-816053-4.09992-2","DOIUrl":"https://doi.org/10.1016/b978-0-12-816053-4.09992-2","url":null,"abstract":"","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89225411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.1016/b978-0-12-816053-4.09995-8
{"title":"Copyright","authors":"","doi":"10.1016/b978-0-12-816053-4.09995-8","DOIUrl":"https://doi.org/10.1016/b978-0-12-816053-4.09995-8","url":null,"abstract":"","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79051829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.1016/b978-0-12-816053-4.00002-x
Mi Zhou, Sarah Weber, Yuanjun Zhao, Han Chen, J. Sundstrom
{"title":"Methods for exosome isolation and characterization","authors":"Mi Zhou, Sarah Weber, Yuanjun Zhao, Han Chen, J. Sundstrom","doi":"10.1016/b978-0-12-816053-4.00002-x","DOIUrl":"https://doi.org/10.1016/b978-0-12-816053-4.00002-x","url":null,"abstract":"","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78908413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-18DOI: 10.1016/B978-0-12-816053-4.00020-1
Ju‐Seop Kang
{"title":"The potential of exosomes as theragnostics in various clinical situations","authors":"Ju‐Seop Kang","doi":"10.1016/B978-0-12-816053-4.00020-1","DOIUrl":"https://doi.org/10.1016/B978-0-12-816053-4.00020-1","url":null,"abstract":"","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76196768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Duchenne muscular dystrophy is the most common type of muscular dystrophy. There is no effective cure for this disease. Recently, researchers have started to look at the therapeutic potential of exosomes — small (40–100 nm) vesicles secreted by cells into the extracellular environment. They transport a few types of macromolecules, including microRNA and proteins, that can be analyzed to estimate the efficacy of the applied therapy. Besides, exosomes can be harnessed for delivering therapeutic components (microRNA, antisense oligonucleotides) to the target tissue. Below, we analyze the available literature and assess the feasibility of using exosomes in the diagnosis and treatment of Duchenne muscular dystrophy. We conclude that exosomes can have their place in the arsenal of researchers and clinicians once some technical issues are solved.
{"title":"The potential of exosomes for the diagnosis and treatment of Duchenne muscular dystrophy","authors":"I. Galkin, T. Egorova","doi":"10.24075/brsmu.2019.049","DOIUrl":"https://doi.org/10.24075/brsmu.2019.049","url":null,"abstract":"Duchenne muscular dystrophy is the most common type of muscular dystrophy. There is no effective cure for this disease. Recently, researchers have started to look at the therapeutic potential of exosomes — small (40–100 nm) vesicles secreted by cells into the extracellular environment. They transport a few types of macromolecules, including microRNA and proteins, that can be analyzed to estimate the efficacy of the applied therapy. Besides, exosomes can be harnessed for delivering therapeutic components (microRNA, antisense oligonucleotides) to the target tissue. Below, we analyze the available literature and assess the feasibility of using exosomes in the diagnosis and treatment of Duchenne muscular dystrophy. We conclude that exosomes can have their place in the arsenal of researchers and clinicians once some technical issues are solved.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85936226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The number of children born with cerebral palsy (CP) remains stably high. Novel approaches for rehabilitation of such patients are being sought. This study aimed to define the efficiency of the image visualization technologies in play activity for the physical rehabilitation of children with cerebral palsy. Sixteen boys with spastic diplegia aged 7–9 participated in the study. They were divided into treatment group (TG) and control group (CG), 8 children each. The TG patients were trained using the virtual reality based Krisaf training simulator twice a week for 40 minutes during 8 months. The child was suspended in the horizontal position and looked at the monitor through the specialised eyeglasses. Under the conditions of the marine environment immersion simulation with reduced gravity children performed motor tasks through play: searched for treasures, competed with dolphins etc. The CG patients attended the physical therapy lessons. Rehabilitation lessons using the virtual reality based Krisaf training simulator for children affected with spastic cerebral palsy led to a significant improvement of motor skills. Various motion tests showed an improvement over baseline, the average indicators increased 1.30–1.48 times. The difference between TG and CG results was statistically significant. In the CG referred to physical therapy the indicators increase was less than 10%, in the TG the increase reached 30–40%. It was concluded that the use of virtual reality based technologies promotes the optimization of neurophysiological processes in the motor analyzer cortical areas and better adaptation to motor loads.
{"title":"Efficiency of image visualization simulator technology for physical rehabilitation of children with cerebral palsy through play","authors":"V. Gorelik, S. Filippova, V. Belyaev, E. Karlova","doi":"10.24075/brsmu.2019.051","DOIUrl":"https://doi.org/10.24075/brsmu.2019.051","url":null,"abstract":"The number of children born with cerebral palsy (CP) remains stably high. Novel approaches for rehabilitation of such patients are being sought. This study aimed to define the efficiency of the image visualization technologies in play activity for the physical rehabilitation of children with cerebral palsy. Sixteen boys with spastic diplegia aged 7–9 participated in the study. They were divided into treatment group (TG) and control group (CG), 8 children each. The TG patients were trained using the virtual reality based Krisaf training simulator twice a week for 40 minutes during 8 months. The child was suspended in the horizontal position and looked at the monitor through the specialised eyeglasses. Under the conditions of the marine environment immersion simulation with reduced gravity children performed motor tasks through play: searched for treasures, competed with dolphins etc. The CG patients attended the physical therapy lessons. Rehabilitation lessons using the virtual reality based Krisaf training simulator for children affected with spastic cerebral palsy led to a significant improvement of motor skills. Various motion tests showed an improvement over baseline, the average indicators increased 1.30–1.48 times. The difference between TG and CG results was statistically significant. In the CG referred to physical therapy the indicators increase was less than 10%, in the TG the increase reached 30–40%. It was concluded that the use of virtual reality based technologies promotes the optimization of neurophysiological processes in the motor analyzer cortical areas and better adaptation to motor loads.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73837832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Evdokimenko, A. Chechetkin, L. D. Druina, M. Tanashyan
Neovascularization of a carotid atherosclerotic plaque (AP) is associated with an increased risk of stroke. Contrast-enhanced ultrasonography (CEUS) is a widely used method for imaging intraplaque neovascularization in vivo. Unfortunately, there are no standardized guidelines for CEUS interpretation. The aim of this study was to identify the most reliable method for CEUS-based assessment of AP neovascularization. Seventy-eight AP were removed during carotid endarterectomy in 73 patients, of whom 5 had AP on both sides, and examined morphologically. All patients underwent preoperative duplex scanning and CEUS; Sonovue was used as a contrast agent. AP neovascularization was assessed on a 4-grade visual scale and with 3 different quantitative methods using QLAB software. On the visual scale (method 1), poorly (37%) and moderately (51%) vascularized plaques were the most common. Quantitative analysis (data were presented as Me (Q1; Q3)) revealed that the number of blood vessels per 1 cm2 of the plaque (method 2) was 16 (10; 26), the ratio of the total vessel area to the plaque area (method 3) was 6% (3; 9), and AP ROI (method 4) was 2.6 dB (1.8; 4.1). Significant correlations were demonstrated between the results produced by method 2 and method 3 (р < 0.0001), method 3 and method 2 (p = 0.0006), and between pathomorphological findings and the results produced by methods 1–3, especially method 2 (p < 0.004). AP ROI brightness did not correlate with other results. The presence of hyperechoic components (calcifications) in AP dramatically reduced the reliability of US-based intraplaque neovascularization assessment. The most accurate CEUS-based quantitative method for assessing intraplaque neovascularization is estimation of blood vessel number per 1 cm2 of the plaque.
颈动脉粥样硬化斑块(AP)的新生血管与卒中风险增加有关。超声造影(CEUS)是一种广泛应用于斑块内新生血管成像的方法。不幸的是，没有标准的超声造影解释指南。本研究的目的是确定最可靠的基于ceus的AP新生血管评估方法。73例患者在颈动脉内膜切除术中切除了78例AP，其中5例患者两侧均有AP，并进行了形态学检查。所有患者术前均行双工扫描和超声造影;Sonovue被用作造影剂。采用QLAB软件，采用4级视觉评分和3种不同的定量方法对AP新生血管进行评估。在目测量表(方法1)上，较差(37%)和中度(51%)血管化斑块最常见。定量分析(数据以Me (Q1;Q3))显示每1 cm2斑块(方法2)的血管数量为16 (10;26)，总血管面积与斑块面积(方法3)之比为6%(方法3;AP ROI (method 4)为2.6 dB (1.8;4.1)。方法2和方法3、方法3和方法2的结果之间存在显著相关性(p < 0.0001)，方法3和方法2的结果之间存在显著相关性(p = 0.0006)，病理形态学结果与方法1-3的结果之间存在显著相关性，特别是方法2 (p < 0.004)。AP ROI亮度与其他结果不相关。AP中高回声成分(钙化)的存在显著降低了基于美国的斑块内新生血管评估的可靠性。评估斑块内新生血管形成最准确的基于ceb的定量方法是估计斑块每1平方厘米的血管数量。
{"title":"Contrast-enhanced ultrasonography for assessing neovascularization of carotid atherosclerotic plaque","authors":"A. Evdokimenko, A. Chechetkin, L. D. Druina, M. Tanashyan","doi":"10.24075/brsmu.2019.057","DOIUrl":"https://doi.org/10.24075/brsmu.2019.057","url":null,"abstract":"Neovascularization of a carotid atherosclerotic plaque (AP) is associated with an increased risk of stroke. Contrast-enhanced ultrasonography (CEUS) is a widely used method for imaging intraplaque neovascularization in vivo. Unfortunately, there are no standardized guidelines for CEUS interpretation. The aim of this study was to identify the most reliable method for CEUS-based assessment of AP neovascularization. Seventy-eight AP were removed during carotid endarterectomy in 73 patients, of whom 5 had AP on both sides, and examined morphologically. All patients underwent preoperative duplex scanning and CEUS; Sonovue was used as a contrast agent. AP neovascularization was assessed on a 4-grade visual scale and with 3 different quantitative methods using QLAB software. On the visual scale (method 1), poorly (37%) and moderately (51%) vascularized plaques were the most common. Quantitative analysis (data were presented as Me (Q1; Q3)) revealed that the number of blood vessels per 1 cm2 of the plaque (method 2) was 16 (10; 26), the ratio of the total vessel area to the plaque area (method 3) was 6% (3; 9), and AP ROI (method 4) was 2.6 dB (1.8; 4.1). Significant correlations were demonstrated between the results produced by method 2 and method 3 (р < 0.0001), method 3 and method 2 (p = 0.0006), and between pathomorphological findings and the results produced by methods 1–3, especially method 2 (p < 0.004). AP ROI brightness did not correlate with other results. The presence of hyperechoic components (calcifications) in AP dramatically reduced the reliability of US-based intraplaque neovascularization assessment. The most accurate CEUS-based quantitative method for assessing intraplaque neovascularization is estimation of blood vessel number per 1 cm2 of the plaque.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78327393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
И. Сашкина, А И Абдуллаева, Г. С. Рунова, И. В. Салдусова, О. В. Зайченко, Д. К. Фасхутдинов, С. И. Соколова, Е. П. Пустовая, A. Abdullaeva, G. S. Runova, I. V. Saldusova, OV Zajchenko, D. Faskhutdinov, S. I. Sokolova, E. P. Pustovaya, Россия Москва, Москва Российский университет дружбы народов, Россия
Chronic generalized periodontitis (CGP) is a disease associated with low susceptibility to the therapeutic protocols applied; practitioners tend to characterize it as a disease presenting certain difficulties. Therefore, the search for drugs and methods capable of increasing the efficacy of CGP therapy is an ongoing process. Additional problems, which have to do with either with antibiotic resistance or increased sensitivity to drugs, also occur quite often. This study aimed to assess the possibility of applying hirudotherapy in the context of conservative treatment of CGP. 50 patients with CGP without somatic pathology were examined and treated. The participants were divided into two groups (n = 25), all group members of about the same age. At the first stage, the treatment followed the accepted standard: professional oral hygiene procedures, antimicrobial and anti-inflammatory drugs, demonstration of proper personal oral hygiene routines. Then, first group went through a monthlong hirudotherapy course that consisted of 6 to 8 individual procedures. Second group was observed throughout this period with the aim to control the level of their compliance with the oral hygiene routines they were trained. Having analyzed the results, we found that hirudotherapy was more effective than what was prescribed to the second (control) group. The papillary marginal alveolar index (PMA), which reflects the severity of inflammation and gum bleeding, decreased significantly in the first group, where medicinal leeches were used: in the patients with severe CGP it went down by 6%, in those with moderately severe CGP the index decreased by 24% and the participants whose CGP was only light had the PMA go down by 2%. Thus, we have demonstrated the efficacy of hirudotherapy in the context of conservative CGP treatment, which allows recommending this method for inclusion into clinical practice.
{"title":"Hirudotherapy in treatment of chronic generalised periodontitis","authors":"И. Сашкина, А И Абдуллаева, Г. С. Рунова, И. В. Салдусова, О. В. Зайченко, Д. К. Фасхутдинов, С. И. Соколова, Е. П. Пустовая, A. Abdullaeva, G. S. Runova, I. V. Saldusova, OV Zajchenko, D. Faskhutdinov, S. I. Sokolova, E. P. Pustovaya, Россия Москва, Москва Российский университет дружбы народов, Россия","doi":"10.24075/brsmu.2019.052","DOIUrl":"https://doi.org/10.24075/brsmu.2019.052","url":null,"abstract":"Chronic generalized periodontitis (CGP) is a disease associated with low susceptibility to the therapeutic protocols applied; practitioners tend to characterize it as a disease presenting certain difficulties. Therefore, the search for drugs and methods capable of increasing the efficacy of CGP therapy is an ongoing process. Additional problems, which have to do with either with antibiotic resistance or increased sensitivity to drugs, also occur quite often. This study aimed to assess the possibility of applying hirudotherapy in the context of conservative treatment of CGP. 50 patients with CGP without somatic pathology were examined and treated. The participants were divided into two groups (n = 25), all group members of about the same age. At the first stage, the treatment followed the accepted standard: professional oral hygiene procedures, antimicrobial and anti-inflammatory drugs, demonstration of proper personal oral hygiene routines. Then, first group went through a monthlong hirudotherapy course that consisted of 6 to 8 individual procedures. Second group was observed throughout this period with the aim to control the level of their compliance with the oral hygiene routines they were trained. Having analyzed the results, we found that hirudotherapy was more effective than what was prescribed to the second (control) group. The papillary marginal alveolar index (PMA), which reflects the severity of inflammation and gum bleeding, decreased significantly in the first group, where medicinal leeches were used: in the patients with severe CGP it went down by 6%, in those with moderately severe CGP the index decreased by 24% and the participants whose CGP was only light had the PMA go down by 2%. Thus, we have demonstrated the efficacy of hirudotherapy in the context of conservative CGP treatment, which allows recommending this method for inclusion into clinical practice.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91288470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lychagin, A. Garkavi, O. Islaieh, P. I. Katunyan, D. Bobrov, R. Yavlieva, E. Tselisheva
Osteoarthritis (OA) affects both elderly people, for whom it is one of the main causes of disability, and people of active working age and is an urgent clinical and social problem of resistance of pain syndrome to therapy. The disease is characterized by both destruction of intra-articular and paraarticular structures, such as subchondral bone. While OA is an important sign of pathological changes believe the bone marrow edema (BME). This work examines the effect of BME on development osteoarthritis, and therapeutic approaches to the management of patients with OA. The aim of the study was to develop a method of treatment of BME in OA of the knee joint by locally intraosseous injection of autologous thrombotic-rich plasma (PRP) into the edema zone. In this study 17 patients with the diagnosis: Osteoarthritis II-IV Grade. according to the classification of Kellgren–Lawrence, in which areas of local inflammation in the form of BME were detected on MRI in the subchondral zone in accordance with the international classification of WORMS (Whole Organ Magnetic Resonance Imaging Score). The mean age of patients was 41,7 ± 14,3 years, 10 of them were women and 7 men. Patients were treated with autological platelet-rich plasma under x-ray control injected from extra-articular intraosseous access in the area of BME. Evaluation of effectiveness of treatment performed by VAS, WOMAC and KOOS scales, before the introduction of autoplasma, after 1 and 3 months after the start of treatment. Three months after the manipulation, there was a statistically significant decrease in the intensity of inflammatory syndrome: for WOMAC by 17.5%, for KOOS by 19.4% and for VAS by 33,1% (p < 0,01). Thus, the efficiency of intraosseous Infiltration of autologous platelet-rich plasma in the treatment of patients with OA, accompanied by edema of the bone marrow in the subchondral zone, was proved.
骨关节炎(OA)既影响老年人，对老年人来说，它是残疾的主要原因之一，也影响积极工作年龄的人，是一个迫切的临床和社会问题，对疼痛综合征的治疗抵抗。这种疾病的特点是破坏关节内和关节旁结构，如软骨下骨。而骨性关节炎是骨髓水肿(BME)病理改变的重要标志。这项工作考察了BME对骨关节炎发展的影响，以及OA患者管理的治疗方法。该研究的目的是通过在水肿区局部骨内注射自体富血栓血浆(PRP)来开发治疗膝关节OA BME的方法。本研究17例患者诊断为II-IV级骨关节炎。根据Kellgren-Lawrence分类，在软骨下区MRI上检测到BME形式的局部炎症区域，按照国际全器官磁共振成像评分(WORMS, Whole Organ Magnetic Resonance Imaging Score)分类。患者平均年龄(41.7±14.3)岁，其中女性10例，男性7例。患者在x线控制下从BME区域的关节外骨内通道注射自体富血小板血浆。采用VAS、WOMAC和oos量表，在引入自体浆前、治疗开始后1个月和3个月对治疗效果进行评价。术后3个月，WOMAC组炎症综合征强度降低17.5%，oos组降低19.4%，VAS组降低33.1%，差异有统计学意义(p < 0.01)。由此，证实了自体富血小板血浆骨内浸润治疗伴有软骨下区骨髓水肿的OA患者的有效性。
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