Pub Date : 2014-01-01DOI: 10.2478/MICRNAT-2013-0002
Xinna Zhang, Xiongbin Lu, G. Lopez-Berestein, A. Sood, G. Calin
Received 05 March 2013 Accepted 31 May 2013 Abstract MicroRNAs (miRNAs) are small non-coding RNAs that regulate various aspects of gene expression in physiology and development. Links between miRNAs and the initiation and progression of human diseases are becoming increasingly apparent. The development of methods to detect the subcellular and tissue localization of miRNAs is essential for understanding their biological role in homeostasis. In this review, we discuss how in situ hybridization can complement tissuelevel miRNA expression profiling and its role as an investigational tool to better understand the etiology of human diseases. Furthermore, in situ hybridization of miRNAs represents a potent diagnostic assay that could be further refined and utilized for clinical applications.
{"title":"In situ hybridization-based detection of microRNAs in human diseases","authors":"Xinna Zhang, Xiongbin Lu, G. Lopez-Berestein, A. Sood, G. Calin","doi":"10.2478/MICRNAT-2013-0002","DOIUrl":"https://doi.org/10.2478/MICRNAT-2013-0002","url":null,"abstract":"Received 05 March 2013 Accepted 31 May 2013 Abstract MicroRNAs (miRNAs) are small non-coding RNAs that regulate various aspects of gene expression in physiology and development. Links between miRNAs and the initiation and progression of human diseases are becoming increasingly apparent. The development of methods to detect the subcellular and tissue localization of miRNAs is essential for understanding their biological role in homeostasis. In this review, we discuss how in situ hybridization can complement tissuelevel miRNA expression profiling and its role as an investigational tool to better understand the etiology of human diseases. Furthermore, in situ hybridization of miRNAs represents a potent diagnostic assay that could be further refined and utilized for clinical applications.","PeriodicalId":90652,"journal":{"name":"microRNA diagnostics and therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/MICRNAT-2013-0002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69232773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-04-26DOI: 10.2478/micrnat-2013-0001
Juan R Cubillos-Ruiz, Melanie R Rutkowski, Julia Tchou, Jose R Conejo-Garcia
It is becoming increasingly clear that there are unique sets of miRNAs that have distinct governing roles in several aspects of both innate and adaptive immune responses. In addition, new tools allow selective modulation of the expression of individual miRNAs, both in vitro and in vivo. Here, we summarize recent advances in our understanding of how miRNAs drive the activity of immune cells, and how their modulation in vivo opens new avenues for diagnostic and therapeutic interventions in multiple diseases, from immunodeficiency to cancer.
{"title":"Reprogramming immune responses via microRNA modulation.","authors":"Juan R Cubillos-Ruiz, Melanie R Rutkowski, Julia Tchou, Jose R Conejo-Garcia","doi":"10.2478/micrnat-2013-0001","DOIUrl":"https://doi.org/10.2478/micrnat-2013-0001","url":null,"abstract":"<p><p>It is becoming increasingly clear that there are unique sets of miRNAs that have distinct governing roles in several aspects of both innate and adaptive immune responses. In addition, new tools allow selective modulation of the expression of individual miRNAs, both in vitro and in vivo. Here, we summarize recent advances in our understanding of how miRNAs drive the activity of immune cells, and how their modulation in vivo opens new avenues for diagnostic and therapeutic interventions in multiple diseases, from immunodeficiency to cancer.</p>","PeriodicalId":90652,"journal":{"name":"microRNA diagnostics and therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/micrnat-2013-0001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32722844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：