Abeer Al-Qatati, E. Abdel-SattarS.Hamad, S. Aliwaini
Background and Objective: Palladacycles have been reported to exert significant anticancer activities against different cancer cells. The current study conducted to evaluate the anti-tumor activity of a new palladacycle complex (ASH10) in estrogen receptor-positive (MCF7) and triple-negative (HCC1937) breast cancer cells. Materials and Methods: The effect of ASH10 on cell proliferation was tested by MTT assay. Scratch assay was used to test the anti-migration ability of ASH10. Apoptosis induced by ASH10 was measured by different methods including nuclear staining and detection of apoptosis markers by western blotting. Autophagy induced by ASH10 also measured by LC3II puncta staining and western blotting using the LC3II antibody. Results: The MTT results showed that the ASH10 compound has a strong anti-growth effect. Data showed that ASH10 induces its cytotoxic effect by inducing DNA damage followed by cell cycle arrest, intrinsic apoptosis and autophagy. Compared to untreated cells, ASH10 treated cells showed high levels of DNA damage markers p-H2AX, p-ATM and p53. Importantly, the cell cycle arrest marker p21 was similarly induced by ASH10 treatment. Furthermore, ASH10 induced significant levels of apoptosis as evidenced by nuclear fragmentation and an increase in the levels of PARP cleavage. Interestingly, ASH10 also activated the formation of autophagosomes and increased the autophagy marker LC3II. Inhibition of autophagy led to a decrease in ASH10 cytotoxicity suggesting that ASH10 induced autophagy was a cytotoxic mechanism. Conclusion: These results demonstrated that ASH10 is a potential effective compound in the treatment of both ER-negative and ER-positive breast cancer cells.
{"title":"Newly Synthesized Palladium (II) Complex ASH10 Induces Apoptosis and Autophagy in Breast Cancer Cells","authors":"Abeer Al-Qatati, E. Abdel-SattarS.Hamad, S. Aliwaini","doi":"10.3923/ijcr.2020.40.47","DOIUrl":"https://doi.org/10.3923/ijcr.2020.40.47","url":null,"abstract":"Background and Objective: Palladacycles have been reported to exert significant anticancer activities against different cancer cells. The current study conducted to evaluate the anti-tumor activity of a new palladacycle complex (ASH10) in estrogen receptor-positive (MCF7) and triple-negative (HCC1937) breast cancer cells. Materials and Methods: The effect of ASH10 on cell proliferation was tested by MTT assay. Scratch assay was used to test the anti-migration ability of ASH10. Apoptosis induced by ASH10 was measured by different methods including nuclear staining and detection of apoptosis markers by western blotting. Autophagy induced by ASH10 also measured by LC3II puncta staining and western blotting using the LC3II antibody. Results: The MTT results showed that the ASH10 compound has a strong anti-growth effect. Data showed that ASH10 induces its cytotoxic effect by inducing DNA damage followed by cell cycle arrest, intrinsic apoptosis and autophagy. Compared to untreated cells, ASH10 treated cells showed high levels of DNA damage markers p-H2AX, p-ATM and p53. Importantly, the cell cycle arrest marker p21 was similarly induced by ASH10 treatment. Furthermore, ASH10 induced significant levels of apoptosis as evidenced by nuclear fragmentation and an increase in the levels of PARP cleavage. Interestingly, ASH10 also activated the formation of autophagosomes and increased the autophagy marker LC3II. Inhibition of autophagy led to a decrease in ASH10 cytotoxicity suggesting that ASH10 induced autophagy was a cytotoxic mechanism. Conclusion: These results demonstrated that ASH10 is a potential effective compound in the treatment of both ER-negative and ER-positive breast cancer cells.","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"10 1","pages":"40-47"},"PeriodicalIF":0.0,"publicationDate":"2020-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81855506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ekbal M. Abo-Has, M. Elwakil, Y. Shabana, M. A. El-Metwall, Ghada El-Kannish, R. Anwar, E. Fawzy, M. Elzayat, Narmin Saied
{"title":"CYP2E1 and L-myc EcoRI Gene Polymorphisms and Heavy Metals Exposure in Hepatocellular Carcinoma Patients","authors":"Ekbal M. Abo-Has, M. Elwakil, Y. Shabana, M. A. El-Metwall, Ghada El-Kannish, R. Anwar, E. Fawzy, M. Elzayat, Narmin Saied","doi":"10.3923/ijcr.2020.48.53","DOIUrl":"https://doi.org/10.3923/ijcr.2020.48.53","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"5 1","pages":"48-53"},"PeriodicalIF":0.0,"publicationDate":"2020-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81875724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asif Rashed, S. Tarafder, Humayun Sattar, Sadia Hossain
{"title":"Expression of Aberrant Antigens in Adult Acute Leukemia: A Study in Bangladesh","authors":"Asif Rashed, S. Tarafder, Humayun Sattar, Sadia Hossain","doi":"10.3923/ijcr.2020.28.34","DOIUrl":"https://doi.org/10.3923/ijcr.2020.28.34","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"451 1","pages":"28-34"},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85309684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. El-Naggar, K. El-Said, Adel Abd Al Hameed Albagoury, Violet Sobhy Awad, L. Attia
{"title":"Cancer-fighting Phytochemicals of Prunus armeniaca and Prunus domestica Seeds Extracts","authors":"S. El-Naggar, K. El-Said, Adel Abd Al Hameed Albagoury, Violet Sobhy Awad, L. Attia","doi":"10.3923/ijcr.2020.10.17","DOIUrl":"https://doi.org/10.3923/ijcr.2020.10.17","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"8 1","pages":"10-17"},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80853128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mujeeb, A. Vedamurthy, Arun K. Shettar, S. Puranik, S. Ghagane, Shivasharana Chandra Thimmappa
{"title":"In vitro Anti-oxidant and Anti-cancer Activity of Tetradesmus acuminatus Microalgae Extract on MCF-7 Human Breast Cancer Cell Line","authors":"M. Mujeeb, A. Vedamurthy, Arun K. Shettar, S. Puranik, S. Ghagane, Shivasharana Chandra Thimmappa","doi":"10.3923/ijcr.2020.1.9","DOIUrl":"https://doi.org/10.3923/ijcr.2020.1.9","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"270 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73367694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanny Nugrahani, A. S. Kusuma, J. Levita, R. Abdulah
{"title":"Synergistic Combination of Sulforaphane and Methylseleninic Acid in Inhibiting the Proliferation of MCF-7 Breast Cancer Cells","authors":"Hanny Nugrahani, A. S. Kusuma, J. Levita, R. Abdulah","doi":"10.3923/ijcr.2020.35.39","DOIUrl":"https://doi.org/10.3923/ijcr.2020.35.39","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"140 1","pages":"35-39"},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86602218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dwi Sutiningsi, Mohamad Arie Wurya, S HenrySetyawan, Sujud Hariyadi, Mustofa
{"title":"Anticancer Activity of Linamarin from Cassava Leaves (Manihot esculenta Cranz) on Raji Cells","authors":"Dwi Sutiningsi, Mohamad Arie Wurya, S HenrySetyawan, Sujud Hariyadi, Mustofa","doi":"10.3923/ijcr.2020.18.27","DOIUrl":"https://doi.org/10.3923/ijcr.2020.18.27","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"7 1","pages":"18-27"},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72644651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objective: MicroRNAs had been implicated in several malignancies. Abnormal circulating microRNA-1246 expression had been detected in HCC patients in an expanding number of studies. However, the information in literature describing the pertinent ramifications of miR-1246 in early-stage HCCs are rare and heterogeneous. This study was designed to assess the diagnostic accuracy of serum miR-1246 level in early-stage HCV-related HCC patients contrasted with chronic hepatitis C (CHC), liver cirrhosis (LC) and healthy control (HC). Materials and Methods: Two hundred HCV outpatients were doled out into 3 groups, HCC group (n = 100), CHC group (n = 30) and LC group (n = 70). Another hundred (100) ageand sex-matched healthy controls (HC) were likewise enlisted. The serum expression level of miR-1246 (by quantitative Real-Time PCR), AFP and prothrombin induced by vitamin K absence-II (PIVKA-II) were tested. Results: In HCC patients, in contrast to AFP, the serum expression levels of PIVKA-II and miR-1246 were statistically significantly increased discriminating HCC patients and early-stage HCCs not only from non-HCC patients (CHC, LC) yet additionally from HC. PIVKA-II and miR-1246, either individually or combined, had excellent diagnostic accuracies and performances as demonstrated by their ROCs and high AUCs >0.7. This serum over-expression positively correlated with the clinicopathological characteristics of both HCC and non-HCC patients. Conclusion: Serum miR-1246 level was significantly higher in HCC patients compared with non-HCC and HC and reliably discriminate early-stage HCV-related HCCs particularly when combined with PIVKA-II.
{"title":"Serum MicroRNA-1246 as a Potential Biomarker for HCV-related Early-stage Hepatocellular Carcinoma","authors":"M. A. Mohammed, N. Omar, S. Mohammed, A. Amin","doi":"10.3923/ijcr.2019.47.57","DOIUrl":"https://doi.org/10.3923/ijcr.2019.47.57","url":null,"abstract":"Background and Objective: MicroRNAs had been implicated in several malignancies. Abnormal circulating microRNA-1246 expression had been detected in HCC patients in an expanding number of studies. However, the information in literature describing the pertinent ramifications of miR-1246 in early-stage HCCs are rare and heterogeneous. This study was designed to assess the diagnostic accuracy of serum miR-1246 level in early-stage HCV-related HCC patients contrasted with chronic hepatitis C (CHC), liver cirrhosis (LC) and healthy control (HC). Materials and Methods: Two hundred HCV outpatients were doled out into 3 groups, HCC group (n = 100), CHC group (n = 30) and LC group (n = 70). Another hundred (100) ageand sex-matched healthy controls (HC) were likewise enlisted. The serum expression level of miR-1246 (by quantitative Real-Time PCR), AFP and prothrombin induced by vitamin K absence-II (PIVKA-II) were tested. Results: In HCC patients, in contrast to AFP, the serum expression levels of PIVKA-II and miR-1246 were statistically significantly increased discriminating HCC patients and early-stage HCCs not only from non-HCC patients (CHC, LC) yet additionally from HC. PIVKA-II and miR-1246, either individually or combined, had excellent diagnostic accuracies and performances as demonstrated by their ROCs and high AUCs >0.7. This serum over-expression positively correlated with the clinicopathological characteristics of both HCC and non-HCC patients. Conclusion: Serum miR-1246 level was significantly higher in HCC patients compared with non-HCC and HC and reliably discriminate early-stage HCV-related HCCs particularly when combined with PIVKA-II.","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87267606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytotoxic Potential and Phytochemical Profile of Extracts from Garcinia rubra Merr. Leaves","authors":"Carlo A. Limbo, S. Jacinto","doi":"10.3923/ijcr.2019.38.46","DOIUrl":"https://doi.org/10.3923/ijcr.2019.38.46","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85074260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Guandalini, L LaísPessanha, Marina Schmidt Mo, Letícia Ribeiro Ca, Ágata Depolo Ech, Naira Marceli Fraga Silv, Glenda Blaser Pet, Taísa Sabrina Silva Pere
{"title":"Neutrophil-to-lymphocyte Ratio and Nutritional Status in Patients with Cancer in Hospital Admission","authors":"V. Guandalini, L LaísPessanha, Marina Schmidt Mo, Letícia Ribeiro Ca, Ágata Depolo Ech, Naira Marceli Fraga Silv, Glenda Blaser Pet, Taísa Sabrina Silva Pere","doi":"10.3923/IJCR.2019.9.16","DOIUrl":"https://doi.org/10.3923/IJCR.2019.9.16","url":null,"abstract":"","PeriodicalId":90856,"journal":{"name":"International journal of cancer research","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86091062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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