Pub Date : 2022-10-04DOI: 10.15583/jpchrom.2022.011
Hsin-Miao Tsai, C. Hsieh, Chiharu Ishii, T. Akita, M. Mita, T. Ide, J. Lee, K. Hamase
{"title":"Two-Dimensional Chiral HPLC Analysis of Lactate, Hydroxybutyrates and Malate in Human Plasma","authors":"Hsin-Miao Tsai, C. Hsieh, Chiharu Ishii, T. Akita, M. Mita, T. Ide, J. Lee, K. Hamase","doi":"10.15583/jpchrom.2022.011","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.011","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48139349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-03DOI: 10.15583/jpchrom.2022.010
Wataru Hobo, Akihiro Jo, Sae Koyagi, Shoujiro Ogawa, S. Nishimoto-Kusunose, T. Higashi
Liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) has the great ability to accurately and precisely quantify various biomolecules, but there is a concern about its analysis time, especially during the analysis of a high number of samples. Sample-multiplexing in the same injection is a promising strategy for reducing the total analysis time. This strategy can be accomplished by derivatization of multiple samples with multiple isotopologous reagents. In this study, a sample-triplex LC/ESI-MS/MS assay was developed for quantifying the urinary hexanoylglycine (HG), a diagnostic marker of medium-chain acyl-coenzyme A dehydrogenase deficiency, in three different samples within a single run. For this purpose, the 1-[(4-diethylaminophenyl)carbonyl]piperazine (DEAPPZ) isotopologues ( 2 H 0 -, 2 H 3 - and 2 H 6 -forms) were synthesized. When compared to the non-derivatization method, which analyzed one sample in each run, the analysis time after the sample pretreatment was reduced to 55% (390 min → 210 min) for 30 samples in the sample-triplex method, which also had an acceptable precision (intra- and inter-assay precisions; ≤ 5.9% and ≤ 9.1%, respectively) and accuracy (91.7–97.1%). Thus, the sample-triplex strategy using the DEAPPZ isotopologues could successfully reduce the analysis time in the urinary HG quantification.
{"title":"An LC/ESI-MS/MS Method for Quantification of Hexanoylglycine in Three Different Urine Samples Within a Single Run Using Triplex 1-[(4- Diethylaminophenyl)carbonyl]piperazine Isotopologues","authors":"Wataru Hobo, Akihiro Jo, Sae Koyagi, Shoujiro Ogawa, S. Nishimoto-Kusunose, T. Higashi","doi":"10.15583/jpchrom.2022.010","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.010","url":null,"abstract":"Liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) has the great ability to accurately and precisely quantify various biomolecules, but there is a concern about its analysis time, especially during the analysis of a high number of samples. Sample-multiplexing in the same injection is a promising strategy for reducing the total analysis time. This strategy can be accomplished by derivatization of multiple samples with multiple isotopologous reagents. In this study, a sample-triplex LC/ESI-MS/MS assay was developed for quantifying the urinary hexanoylglycine (HG), a diagnostic marker of medium-chain acyl-coenzyme A dehydrogenase deficiency, in three different samples within a single run. For this purpose, the 1-[(4-diethylaminophenyl)carbonyl]piperazine (DEAPPZ) isotopologues ( 2 H 0 -, 2 H 3 - and 2 H 6 -forms) were synthesized. When compared to the non-derivatization method, which analyzed one sample in each run, the analysis time after the sample pretreatment was reduced to 55% (390 min → 210 min) for 30 samples in the sample-triplex method, which also had an acceptable precision (intra- and inter-assay precisions; ≤ 5.9% and ≤ 9.1%, respectively) and accuracy (91.7–97.1%). Thus, the sample-triplex strategy using the DEAPPZ isotopologues could successfully reduce the analysis time in the urinary HG quantification.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48171875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-20DOI: 10.15583/jpchrom.2022.005
Takako Hayashi, K. Hamase
{"title":"Simultaneous Determination of Seven β2-Agonists in Livestock Products Using an LC-MS/MS System","authors":"Takako Hayashi, K. Hamase","doi":"10.15583/jpchrom.2022.005","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.005","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43316231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-13DOI: 10.15583/jpchrom.2022.009
Kohei Kawabata, M. Iwata, Manae Kawaguchi, M. Kaneko, Mami Gennai, Shiori Akimoto, Masanori Inagaki, Keiichi Segawa, H. Nishi
{"title":"Photostabilities of Amlodipine OD Tablets in Different Dosage Forms","authors":"Kohei Kawabata, M. Iwata, Manae Kawaguchi, M. Kaneko, Mami Gennai, Shiori Akimoto, Masanori Inagaki, Keiichi Segawa, H. Nishi","doi":"10.15583/jpchrom.2022.009","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.009","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42768938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-06DOI: 10.15583/jpchrom.2022.008
K. Todoroki, Ryota Isobe, Tomohiro Yamada, T. Saito, Yutaka Shimizu, K. Tsukakoshi, Eiji Sugiyama, H. Mizuno, H. Hayashi, K. Ikebukuro
{"title":"Development of a Lateral Flow Assay for Bevacizumab Using an Anti-Idiotype DNA Aptamer as a Capture Molecule","authors":"K. Todoroki, Ryota Isobe, Tomohiro Yamada, T. Saito, Yutaka Shimizu, K. Tsukakoshi, Eiji Sugiyama, H. Mizuno, H. Hayashi, K. Ikebukuro","doi":"10.15583/jpchrom.2022.008","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.008","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47011547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-03DOI: 10.15583/jpchrom.2022.007
Ren Sakita, Hiroka Kishi, Yoshinori Iiguni, S. Kitagawa, H. Ohtani
{"title":"Fundamental Study for Increasing Multiplicity of Frequency Division Multiplex HPLC-MS","authors":"Ren Sakita, Hiroka Kishi, Yoshinori Iiguni, S. Kitagawa, H. Ohtani","doi":"10.15583/jpchrom.2022.007","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.007","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46540172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-31DOI: 10.15583/jpchrom.2022.006
Mai Oyaide, Chiharu Ishii, A. Fujii, T. Akita, M. Mita, M. Nagano, K. Hamase
{"title":"Enantioselective Determination of Hydroxy Amino Acids in Japanese Traditional Amber Rice Vinegars","authors":"Mai Oyaide, Chiharu Ishii, A. Fujii, T. Akita, M. Mita, M. Nagano, K. Hamase","doi":"10.15583/jpchrom.2022.006","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.006","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49113423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-06DOI: 10.15583/jpchrom.2022.003
I. Ueta, A. Miyazaki, S. Yano, Ryosuke Kikuchi, Koki Nakagami, Yoshihiro Saito, K. Kawata
{"title":"Evaluation of Volatility of Bendamustine Using Solid Phase Extraction-Type Extraction Device","authors":"I. Ueta, A. Miyazaki, S. Yano, Ryosuke Kikuchi, Koki Nakagami, Yoshihiro Saito, K. Kawata","doi":"10.15583/jpchrom.2022.003","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.003","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48845395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-06DOI: 10.15583/jpchrom.2022.004
Yohei Mikami, Mizuo Aizawa, Ryoko Toda, Shoujiro Ogawa, S. Nishimoto-Kusunose, T. Ishige, T. Higashi
{"title":"Application of 4-Diethylaminobenzoic Acid N-Succinimidyl Ester and Its Deuterated Isotopologue as Derivatization Reagents to Quantitative Analysis of γ-Aminobutyric Acid in Serum by LC/ESI-MS/MS","authors":"Yohei Mikami, Mizuo Aizawa, Ryoko Toda, Shoujiro Ogawa, S. Nishimoto-Kusunose, T. Ishige, T. Higashi","doi":"10.15583/jpchrom.2022.004","DOIUrl":"https://doi.org/10.15583/jpchrom.2022.004","url":null,"abstract":"","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46517458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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