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Evaluating anthelmintic, anti-platelet, and anti-coagulant activities, and identifying the bioactive phytochemicals of Amaranthus blitum L. 评价马齿苋的抗蠕虫、抗血小板和抗凝血活性,并鉴定其生物活性植物化学物质
2区 医学 Q1 Medicine Pub Date : 2024-05-04 DOI: 10.1186/s12906-024-04478-2
Ghada Abdel-Moez, Hanaa Sayed, Azza Khalifa, Salwa Abd-Elrahman, Mohammed Osman, Shaymaa Mohamed
Highlighting affordable alternative crops that are rich in bioactive phytoconstituents is essential for advancing nutrition and ensuring food security. Amaranthus blitum L. (AB) stands out as one such crop with a traditional history of being used to treat intestinal disorders, roundworm infections, and hemorrhage. This study aimed to evaluate the anthelmintic and hematologic activities across various extracts of AB and investigate the phytoconstituents responsible for these activities. In vitro anthelmintic activity against Trichinella spiralis was evaluated in terms of larval viability reduction. The anti-platelet activities were assessed based on the inhibitory effect against induced platelet aggregation. Further, effects on the extrinsic pathway, the intrinsic pathway, and the ultimate common stage of blood coagulation, were monitored through measuring blood coagulation parameters: prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT), respectively. The structures of isolated compounds were elucidated by spectroscopic analysis. Interestingly, a previously undescribed compound (19), N-(cis-p-coumaroyl)-ʟ-tryptophan, was isolated and identified along with 21 known compounds. Significant in vitro larvicidal activities were demonstrated by the investigated AB extracts at 1 mg/mL. Among tested compounds, compound 18 (rutin) displayed the highest larvicidal activity. Moreover, compounds 19 and 20 (N-(trans-p-coumaroyl)-ʟ-tryptophan) induced complete larval death within 48 h. The crude extract exhibited the minimal platelet aggregation of 43.42 ± 11.69%, compared with 76.22 ± 14.34% in the control plasma. Additionally, the crude extract and two compounds 19 and 20 significantly inhibited the extrinsic coagulation pathway. These findings extend awareness about the nutritional value of AB as a food, with thrombosis-preventing capabilities and introducing a promising source for new anthelmintic and anticoagulant agents.
突出经济实惠、富含生物活性植物成分的替代作物对于促进营养和确保粮食安全至关重要。Amaranthus blitum L.(AB)就是这样一种作物,具有治疗肠道疾病、蛔虫感染和出血的传统历史。本研究旨在评估马齿苋各种萃取物的驱虫和血液学活性,并调查导致这些活性的植物成分。体外抗蠕虫活性是根据幼虫存活率的降低来评估的。根据对诱导血小板聚集的抑制作用评估了抗血小板活性。此外,通过测量血液凝固参数:凝血酶原时间(PT)、活化部分凝血活酶时间(aPTT)和凝血酶时间(TT),分别监测了对血液凝固的外在途径、内在途径和最终共同阶段的影响。通过光谱分析阐明了分离化合物的结构。有趣的是,分离出了一种以前未曾描述过的化合物(19),即 N-(顺式-对香豆酰基)-ʟ-色氨酸,并与 21 种已知化合物一起进行了鉴定。所研究的 AB 提取物在 1 mg/mL 的浓度下具有显著的体外杀幼虫剂活性。在测试的化合物中,化合物 18(芦丁)的杀幼虫活性最高。此外,化合物 19 和 20(N-(反式对香豆酰基)-ʟ-色氨酸)可在 48 小时内诱导幼虫完全死亡。粗提取物的血小板聚集率最低,为 43.42 ± 11.69%,而对照血浆的血小板聚集率为 76.22 ± 14.34%。此外,粗提取物和两种化合物 19 和 20 还能明显抑制外凝血途径。这些发现扩大了人们对 AB 作为食物的营养价值的认识,它具有预防血栓形成的功能,并为新的抗虫药和抗凝血剂提供了一个前景广阔的来源。
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引用次数: 0
Views of Hong Kong Chinese medicine practitioners on the application of the “Chinese Medicine Anti-epidemic Plans” prepared by the Chinese medicine expert group of central authorities: a focus group study 香港中医师对应用中央中医药专家组编制的 "中医药防疫计划 "的意见:焦点小组研究
2区 医学 Q1 Medicine Pub Date : 2024-05-04 DOI: 10.1186/s12906-024-04469-3
Shu Cheng Chen, Wing Fai Yeung, Hui Lin Cheng, Man Ho Li, Yuen Shan Ho
Drawing on the extensive utilization of traditional Chinese medicine (TCM) to combat COVID-19 in Mainland China, experts designed a series of TCM anti-epidemic strategies. This study aims to understand Hong Kong CM practitioners’ application of and opinions on the “Chinese Medicine Anti-epidemic Plans.” Online focus group interviews were conducted, and purposive sampling was employed to invite 22 CM practitioners to voluntarily participate in three interview sessions. The interviews were audio recorded, then transcribed verbatim. The transcripts were analyzed using template analysis. Three themes were derived: (1) facilitators of the “Chinese Medicine Anti-epidemic Plans,” (2) barriers of the “Chinese Medicine Anti-epidemic Plans,” and (3) expectations on improving the “Chinese Medicine Anti-epidemic Plans.” The participants could obtain relevant information from various sources, which highlights the value of the plans for TCM medicinal cuisine and non-pharmacologic therapies and guiding junior CM practitioners, supplementing Western medicine interventions, and managing Chinese herb reserves in clinics. However, the barriers included the lack of a specialized platform for timely information release, defective plan content, limited reference value to experienced CM practitioners, and lack of applicability to Hong Kong. The expectations of the CM practitioners for improving the plans were identified based on the barriers. To enhance the implementation of the anti-epidemic plans, CM practitioners in Hong Kong expect to utilize a specific CM platform and refine the plans to ensure that they are realistic, focused, comprehensive, and tailored to the local context.
根据中国大陆广泛使用传统中医药防治 COVID-19 的经验,专家们设计了一系列中医药抗疫策略。本研究旨在了解香港中医师对 "中医药抗疫计划 "的应用及意见。本研究以网上焦点小组访谈形式进行,并采用目的性抽样,邀请 22 位中医师自愿参与三次访谈。访谈进行了录音,然后逐字记录。访谈记录采用模板分析法进行分析。得出三个主题:(1)"中医药防疫计划 "的促进因素;(2)"中医药防疫计划 "的障碍;(3)对改进 "中医药防疫计划 "的期望。与会者可以从各种渠道获得相关信息,这凸显了中医药药膳和非药物疗法计划的价值,以及对初级中医师的指导、对西医干预措施的补充和诊所中草药储备的管理。然而,障碍包括缺乏及时发布信息的专业平台、计划内容有缺陷、对有经验的中医师的参考价值有限以及缺乏对香港的适用性。根据这些障碍,确定了中药从业人员对改进计划的期望。为加强防疫计划的实施,香港的中药从业人员期望利用专门的中药平台,完善防疫计划,确保防疫计划切实可行、重点突出、内容全面,并符合本地实际情况。
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引用次数: 0
Effects of hand-press pellet on pain and daily life of elders with chronic lower back pain: randomized controlled trial 手压颗粒对慢性下背痛老人的疼痛和日常生活的影响:随机对照试验
2区 医学 Q1 Medicine Pub Date : 2024-05-03 DOI: 10.1186/s12906-024-04481-7
Hyojung Park, Hyejin Lee
For elderly people with chronic lower back pain who need long-term management, there is a need for a nursing intervention study that is effective, is easy to perform, and applies complementary and alternative therapies to manage pain without repulsion. Hand pressure therapy is a treatment indigenous to Korea used to reduce pain and improve functions of daily life by applying acupuncture, pressure sticks, and moxibustion to parts of the hand as they relate to parts of the body. This research is to identify the effects of pellet pressed on the hand on pain and the daily lives of elders with chronic lower back pain (CLBP). The hand pressed-pellet intervention period was six weeks long. Twenty-seven patients in the intervention group and twenty-four patients in the placebo control group were recruited from elderly over sixty-five who used welfare centers. In the intervention group, hand pressed-pellet therapy was conducted in eleven acupressure response zones related to CLBP, and the placebo control group was provided with similar therapy and zones, but unrelated to CLBP. The research tool measured the intensity of CLBP using the Visual Analogue Scale (VAS), the Korean Owestry Disability Index (K-ODI), which are subjective indicators, and the Compact Digital Algometer, which is an objective indicator. The pain intensity (VAS) measured after six weeks of hand pressed-pellet therapy showed significant difference between the two groups compared to their pain before the experiment (F = 60.522, p < .001). There was a significant difference between the two groups in the pain pressure threshold using pressure statistics (F = 8.940, p < .001), and in CLBP dysfunction evaluation index (K-ODI) after applying pressed pellet to the hand (Z = − 3.540, p < .001). Subjective indicators were measured to verify the effect of hand pressed-pellet therapy on CLBP, and the result confirmed that the hand pressed-pellet therapy was effective in alleviating CLBP. The study was registered retrospectively with reference number KCT0008024 on 23/12/2022.
对于需要长期治疗的患有慢性腰背痛的老年人来说,有必要开展一项有效、易于实施、应用辅助和替代疗法来控制疼痛而不产生排斥反应的护理干预研究。手压疗法是韩国本土的一种治疗方法,通过针灸、压棒和艾灸手部与身体各部位的关系来减轻疼痛和改善日常生活功能。本研究旨在确定手部按压颗粒对慢性腰背痛(CLBP)老人的疼痛和日常生活的影响。手部按压颗粒干预期为六周。干预组和安慰剂对照组分别有 27 名和 24 名患者,他们都是从使用福利中心的 65 岁以上老人中招募的。干预组在与慢性前列腺炎相关的 11 个穴位反应区进行手按颗粒疗法,安慰剂对照组提供类似的疗法和区段,但与慢性前列腺炎无关。研究工具采用主观指标视觉模拟量表(VAS)、韩国奥维司特里残疾指数(K-ODI)和客观指标紧凑型数字测力计来测量 CLBP 的强度。手压颗粒疗法六周后测量的疼痛强度(VAS)显示,两组患者的疼痛强度与实验前相比有显著差异(F = 60.522,P < .001)。使用压力统计法测量的疼痛压力阈值(F = 8.940,p < .001)和手部按压颗粒后的 CLBP 功能障碍评估指数(K-ODI)(Z = - 3.540,p < .001)在两组之间存在显著差异。通过测量主观指标来验证手部按压颗粒疗法对 CLBP 的效果,结果证实手部按压颗粒疗法能有效缓解 CLBP。该研究于 2022 年 12 月 23 日进行了回顾性注册,注册编号为 KCT0008024。
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引用次数: 0
PRECIS-2 analysis of pragmatic acupuncture trials: a systematic review PRECIS-2 实用针灸试验分析:系统回顾
2区 医学 Q1 Medicine Pub Date : 2024-05-03 DOI: 10.1186/s12906-024-04473-7
Jinwoong Lim, Hyeonhoon Lee, Yong-Suk Kim
Pragmatic acupuncture trials (PATs) are a research tool for assessing the effectiveness of acupuncture treatments in a real-world setting. This study aimed to provide a comprehensive methodological analysis of PATs using the PRECIS-2(PRagmatic Explanatory Continuum Indicator Summary-2) tool to determine their pragmatism. The MEDLINE, EMBASE, Cochrane Central Register for Controlled Trials, CINAHL, Allied and Complementary Medicine Database, China National Knowledge Infrastructure, VIP, WANFANG, Taiwan Periodical Literature Database, KoreaMed, KMbase, Research Information Service System, Oriental Medicine Advanced Searching Integrated System, CiNii and ClinicalTrials.gov were searched. The search included randomised controlled trials (RCTs) and protocols of RCTs that investigated all types of acupuncture and used self-declared pragmatic design. Two authors independently collected the basic information and characteristics of the studies and assessed their pragmatism using the nine PRECIS-2 domains and the additional domain of control. A total of 93 studies were included. The means of eligibility, recruitment, organisation, primary outcome, primary analysis, and control domains were statistically larger than three and were shown to be pragmatic. The means of setting, flexibility:delivery, and follow-up domains were not greater than three and were shown to be non-pragmatic. For flexibility:adherence domain was inappropriate for assessment owing to insufficient information in the studies. PATs were pragmatic in the domain of eligibility, recruitment, organisation, primary outcome, primary analysis, and control and were not pragmatic in the domain of setting, flexibility:delivery, and follow-up. Future PATs need to strengthen the pragmatism in the setting, flexibility:delivery, and follow-up domains and to describe the flexibility:adherence domain in more detail. CRD42021236975.
实用针灸试验(PATs)是一种在真实世界环境中评估针灸治疗效果的研究工具。本研究旨在使用 PRECIS-2(PRagmatic Explanatory Continuum Indicator Summary-2)工具对 PAT 进行全面的方法学分析,以确定其实用性。本研究检索了MEDLINE、EMBASE、Cochrane对照试验中央注册中心、CINAHL、专科和补充医学数据库、中国国家知识基础设施、VIP、万方数据库、台湾期刊文献数据库、KoreaMed、KMbase、研究信息服务系统、东方医学高级检索集成系统、CiNii和ClinicalTrials.gov。搜索范围包括研究所有类型针灸并采用自我声明的实用主义设计的随机对照试验(RCT)和 RCT 方案。两位作者独立收集了研究的基本信息和特征,并使用 PRECIS-2 的九个领域和控制的附加领域对其实用性进行了评估。共有 93 项研究被纳入其中。资格、招募、组织、主要结果、主要分析和控制领域的平均值在统计上大于 3,并被证明是实用的。设置、灵活性:提供和随访领域的均值均未超过 3,被证明是非实用性的。由于研究信息不足,灵活性:坚持领域不适合进行评估。PAT 在资格、招募、组织、主要结果、主要分析和控制方面是务实的,而在设置、灵活性:实施和随访方面则不务实。未来的 PAT 需要加强设置、灵活性:交付和随访领域的实用性,并更详细地描述灵活性:依从性领域。CRD42021236975。
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引用次数: 0
Antibacterial activity of Dioscorea bulbifera Linn. extract and its active component flavanthrinin against skin-associated bacteria 薯蓣提取物及其活性成分黄连素对皮肤相关细菌的抗菌活性
2区 医学 Q1 Medicine Pub Date : 2024-05-02 DOI: 10.1186/s12906-024-04480-8
Donruedee Sanguansermsri, Phanchana Sanguansermsri, Kittisak Buaban, Vachira Choommongkol, Chareeporn Akekawatchai, Noree Charoensri, Ian Fraser, Nalin Wongkattiya
Dioscorea bulbifera Linn. has been used for wound care in Thailand. However, a comprehensive evaluation of its antibacterial activity is required. This study aimed to investigate the antibacterial efficacy of D. bulbifera extract against skin-associated bacteria and isolate and characterize its active antibacterial agent, flavanthrinin. Air-dried bulbils of D. bulbifera were pulverised and extracted with hexane, dichloromethane, ethyl acetate, methanol, ethanol, and distilled water; vacuum filtered; concentrated; freeze-dried; and stored at -20 ºC. Antibacterial activity of the extracts was assessed using microdilution techniques against several skin-associated bacteria. Thin-layer chromatography (TLC) bioautography was used to identify the active compounds in the extract, which were fractionated by column chromatography and purified by preparative TLC. The chemical structures of the purified compounds were analysed using nuclear magnetic resonance (NMR). The cytotoxicity of the extract and its active compounds was evaluated in Vero cells. The ethyl acetate extract exhibited distinct inhibition zones against bacteria compared to other extracts. Therefore, the ethyl acetate extract of D. bulbifera in the ethyl acetate layer was used for subsequent analyses. D. bulbifera extract exhibited antibacterial activity, with minimum inhibitory concentrations (MICs) of 0.78–1.56 mg/mL. An active compound, identified through TLC-bioautography, demonstrated enhanced antibacterial activity, with MICs of 0.02–0.78 mg/mL. NMR analysis identified this bioactive compound as flavanthrinin. Both D. bulbifera extract and flavanthrinin-containing fraction demonstrated potent antibacterial activity against Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), and S. epidermidis. The flavanthrinin containing fraction demonstrated low cytotoxicity against Vero cells, showing CC50 values of 0.41 ± 0.03 mg/mL. These values are lower than the MIC value, indicating that this fraction is safer than the initial ethyl acetate extract. Dioscorea bulbifera extract and its bioactive component flavanthrinin demonstrated significant antibacterial activity against the skin-associated bacteria Staphylococci, including MRSA. Flavanthrinin has potential as a complementary therapeutic agent for managing skin infections owing to its potent antibacterial effects and low cytotoxicity.
在泰国,薯蓣(Dioscorea bulbifera Linn.)已被用于伤口护理。然而,需要对其抗菌活性进行全面评估。本研究旨在调查球茎薯蓣萃取物对皮肤相关细菌的抗菌功效,并分离和鉴定其活性抗菌剂黄黄连素。将风干的球茎粉碎,用正己烷、二氯甲烷、乙酸乙酯、甲醇、乙醇和蒸馏水提取,真空过滤,浓缩,冷冻干燥,储存于 -20 ºC。使用微量稀释技术评估了提取物对几种皮肤相关细菌的抗菌活性。采用薄层色谱(TLC)生物自动层析技术确定了提取物中的活性化合物,并通过柱层析和制备型 TLC 进行了分馏和纯化。利用核磁共振(NMR)分析了纯化化合物的化学结构。在 Vero 细胞中评估了提取物及其活性化合物的细胞毒性。与其他提取物相比,乙酸乙酯提取物对细菌的抑制作用明显。因此,在随后的分析中使用了醋酸乙酯层中的 D. bulbifera 提取物。D.bulbifera提取物具有抗菌活性,最低抑菌浓度(MIC)为0.78-1.56 mg/mL。通过 TLC 生物指纹图谱鉴定出的一种活性化合物显示出更强的抗菌活性,最低抑菌浓度为 0.02-0.78 mg/mL。核磁共振分析确定这种生物活性化合物为黄花菜素。D.bulbifera萃取物和含黄连素的馏分都对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌(MRSA)和表皮葡萄球菌具有很强的抗菌活性。含黄素的馏分对 Vero 细胞的细胞毒性较低,CC50 值为 0.41 ± 0.03 mg/mL。这些值低于 MIC 值,表明该馏分比最初的乙酸乙酯提取物更安全。薯蓣提取物及其生物活性成分黄连素对皮肤相关细菌葡萄球菌(包括 MRSA)具有显著的抗菌活性。由于黄花菜素具有强大的抗菌效果和较低的细胞毒性,因此有望成为治疗皮肤感染的辅助治疗剂。
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引用次数: 0
Response to “Physical activity and psychological support can replace “another pill” to manage cancer-related symptoms in children and adolescents diagnosed with cancer” 对 "体育锻炼和心理支持可取代 "另一种药片 "来控制确诊癌症的儿童和青少年的癌症相关症状 "的回应
2区 医学 Q1 Medicine Pub Date : 2024-04-22 DOI: 10.1186/s12906-024-04476-4
Dana C. Mora, Miek C. Jong, Sara A. Quandt, Thomas A. Arcury, Agnete E. Kristoffersen, Trine Stub

Thank you for the opportunity to respond to the letter to the editor submitted by Dr. Caru and his colleagues [1]. We appreciate the interest of Dr. Caru et al. in our paper and their knowledgeable comments on our study.

In their letter to the editor [1], Dr. Caru notes that the findings of our study [2] need to be put into perspective to increase its scope and highlight that physical activity and psychosocial interventions are important nonpharmacological interventions to manage cancer-related symptoms. Furthermore, they point out that our paper [2] did not discuss physical activity and psychological support as CAM modalities. We agree that the latter were not discussed in the paper to a great extent. However, this reflects the use of a qualitative interview design in which we focused on modalities that the participants themselves discussed in response to the queries and probes used to illicit their views on supportive care for children undergoing cancer treatment. The interviews were open-ended, and, while participants’ responses were probed for additional modalities, such an approach does not query about specific modalities not discussed by the participants. Some participants briefly mentioned physical activity, but it was not spoken about in enough detail or by enough participants to emerge as a salient theme in the data analysis. We briefly discuss psychosocial support in the results section under the play, psychodrama, and music therapy Sect. [2].

As stated in the paper’s methods section, the participants were recruited using purposive sampling [3] through professional pediatrics and CAM networks. One of the limitations of the paper was that it was not possible to interview providers for every CAM modality currently used. We agree that our study had limitations and think it is important to highlight modalities (such as physical activity and psychosocial interventions) that are safe and effective and can aid parents and children in coping with the adverse effects derived from cancer treatment.

In their letter, Dr. Caru and colleagues appear to criticize our paper because it did not include experts on physical activity. This criticism misses the point that we interviewed care providers with expetice in treating pediatric oncology patients not necessarily researchers. We do understand that there have been studies published of the effectiveness of physical activity interventions with child cancer patients [4]. However, this does not necessarily make such interventions top of mind with care providers, the population we interviewed. It is indeed significant that such interventions were NOT noted by our study participants. We suggest that this reflects the need for greater training of care providers in the effectiveness of such modalities, rather than a shortcoming of our study.

In conclusion, we agree with Dr. Caru and his colleagues that physical activity and psychosocial interventions are important nonpharmacological

感谢您让我们有机会对 Caru 博士及其同事提交的致编辑的信[1]做出回应。我们感谢 Caru 博士等人对我们论文的关注以及他们对我们研究提出的富有见地的意见。Caru 博士在致编辑的信[1]中指出,我们的研究结果[2]需要从更广阔的角度来看待,并强调体育锻炼和社会心理干预是控制癌症相关症状的重要非药物干预措施。此外,他们还指出,我们的论文[2]并未将体育锻炼和心理支持作为 CAM 方式进行讨论。我们同意该论文在很大程度上没有讨论后者。不过,这反映出我们采用了定性访谈设计,在访谈中,我们重点关注参与者在回答询问和探究时自己讨论的方式,以了解他们对接受癌症治疗的儿童的支持性护理的看法。访谈是开放式的,虽然我们会在参与者的回答中询问其他方式,但这种方法不会询问参与者未讨论过的具体方式。一些参与者简短地提到了体育锻炼,但由于谈论的不够详细,也没有足够多的参与者将其作为数据分析中的一个突出主题。我们在结果部分的游戏、心理剧和音乐疗法部分简要讨论了社会心理支持。[正如本文方法部分所述,参与者是通过专业儿科和 CAM 网络以目的性抽样[3]的方式招募的。本文的局限性之一是无法采访到目前使用的每一种 CAM 方式的提供者。我们同意我们的研究存在局限性,并认为强调安全有效的方式(如体育锻炼和心理干预)非常重要,这些方式可以帮助家长和儿童应对癌症治疗带来的不良影响。在他们的信中,卡鲁博士及其同事似乎批评我们的论文,因为其中没有包括体育锻炼方面的专家。这种批评忽略了一点,即我们采访的是具有治疗儿科肿瘤患者经验的护理人员,而不一定是研究人员。我们确实知道,已经有关于儿童癌症患者体育锻炼干预效果的研究发表[4]。然而,这并不一定会让我们的受访者--医疗服务提供者将此类干预措施放在首位。我们的研究参与者并未注意到此类干预措施,这一点确实很重要。总之,我们同意 Caru 博士及其同事的观点,即体育锻炼和社会心理干预是控制儿童和青少年癌症相关症状的重要非药物干预措施。我们很高兴他们对我们的工作产生了兴趣,并扩大了工作范围,因为这丰富了研究领域,并进一步强调了有助于减轻确诊癌症的儿童及其家人必须承受的负担的方式。体育锻炼和心理支持可替代另一种药片,帮助确诊癌症的儿童和青少年控制癌症相关症状。2023.Mora DC、Jong MC、Quandt SA、Arcury TA、Kristoffersenn AE、Stub T.《儿科肿瘤中癌症相关症状的支持性治疗:医疗服务提供者的定性研究》。BMC Complement Altern Med 2023, 23(104).Patton MQ.定性研究与amp;评估方法:理论与实践相结合》。加利福尼亚州:加州:Sage;2014.Google Scholar Wurz A、McLaughlin E、Lategan C、Ellis K、Culos-Reed SN.综合受癌症影响的儿童和青少年的体育锻炼文献:国际儿科肿瘤锻炼指南(iPOEG)的证据》。Transl Behav Med.2021;11(3):699-708.Article PubMed PubMed Central Google Scholar Download referencesNot applicable.Not applicable.Authors and AffiliationsNational Research Center in Complementary and Alternative Medicine, Department of Community Medicine, Faculty of Health Science, NAFKAM, UiT The Arctic University of Norway, Tromsø, 9037, NorwayDana C. Mora, Miek C. Jong, Agnete E. Kristoffersen &amp; N.C. M. Jong.Kristoffersen &amp; Trine StubDepartment of Epidemiology and Prevention, Division of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USASara A. QuandtDepartment of Family and Community Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USAThomas A. ArcuryAuthorsDana C. Mora查看作者发表的文章您也可以在PubMed Google Scholar中搜索该作者Miek C. Jong, Agnete E. Kristoffersen &amp; Trine StubDepartment of Epidemiology and Prevention, Division of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USAThomas A. Arcury JongView 作者发表作品您也可以在 PubMed Google ScholarSara A. QuandtView 作者发表作品您也可以在 PubMed Google ScholarThomas A. ArcuryView 作者发表作品您也可以在 PubMed Google ScholarAgnete E. KristoffersenView 作者发表作品您也可以在 PubMed Google ScholarTrine Stubiew 作者发表作品您也可以在 PubMed Google ScholarCributionsKristoffersenView author publications您也可以在PubMed Google Scholar中搜索该作者Trine StubView author publications您也可以在PubMed Google Scholar中搜索该作者ContributionsAll author reviewed the response.通讯作者Dana C. Mora.伦理批准Not applicable.同意参与Not applicable.同意发表Not applicable.同意出版Not applicable.同意发表不适用.利益冲突/竞争性利益作者声明他们没有利益冲突,也没有竞争性利益.出版商注释Springer Nature对出版地图中的管辖权主张和机构隶属关系保持中立。开放获取 本文采用知识共享署名 4.0 国际许可协议进行许可,该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文,但必须注明原作者和出处,提供知识共享许可协议的链
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引用次数: 0
Retraction Note: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity 撤稿说明:从 Mitrella kentii 中分离出的 desmosdumotin C 对乙醇诱导的大鼠胃粘膜出血的胃保护作用:谷胱甘肽、热休克蛋白-70、巯基化合物、一氧化氮和抗幽门螺杆菌活性的可能参与
2区 医学 Q1 Medicine Pub Date : 2024-04-19 DOI: 10.1186/s12906-024-04475-5
Heyam Mohamed Ali Sidahmed, Ainnul Hamidah Syahadah Azizan, Syam Mohan, Mahmood Ameen Abdulla, Siddig Ibrahim Abdelwahab, Manal Mohamed Elhassan Taha, A. Hamid A. Hadi, Kamal Aziz Ketuly, Najihah Mohd Hashim, Mun Fai Loke, Jamuna Vadivelu

Retraction Note: BMC Complement Med Ther 13, 183 (2013)

https://doi.org/10.1186/1472-6882-13-183

The Editors retracted this article because of concerns regarding a number of figures presented in this work. These concerns call into question the article’s overall scientific soundness and its authorship. An investigation conducted after its publication discovered similarities between images in this work and images in [1] and [2],

  • Panel D in Fig. 5 and panel B in Fig. 5 in [1];

  • Panel D in Fig. 6 and panel E in Fig. 5 in [2];

  • Panel F in Fig. 5 and panel E in Fig. 4 in [1];

  • The bottom part of panel F in Fig. 5 and the top part of panel E in Fig. 4 in [2];

The Editors therefore no longer have confidence in the integrity of the research presented in this article. Jamuna Vadivelu and Mun Fai Loke agree with the retraction. The remaining authors have not responded to correspondence from the Publisher about the retraction.

  1. Al Batran R, Al-Bayaty F, Ameen Abdulla M, Al-Obaidi J, Hajrezaei MM, Hassandarvish M, Fouad P, Golbabapour M, S. and, Talaee S. Gastroprotective effects of Corchorus olitorius. J Gastroenterol Hepatol. 2013;28:1321–9. https://doi.org/10.1111/jgh.12229.

    Article PubMed PubMed Central Google Scholar

  2. Ismail IF, Golbabapour S, Hassandarvish P, Hajrezaie M, Majid NA, Kadir FA. Fouad Al-Bayaty, Khalijah Awang, Hazrina Hazni, Mahmood Ameen Abdulla, Gastroprotective Activity of Polygonum chinense Aqueous Leaf Extract on Ethanol-Induced Hemorrhagic Mucosal Lesions in Rats, Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 404012, 9 pages, 2012. https://doi.org/10.1155/2012/404012.

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Author notes
  1. Equal contributors.

Authors and Affiliations

  1. Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia

    Heyam Mohamed Ali Sidahmed, Syam Mohan & Najihah Mohd Hashim

  2. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia

    Mahmood Ameen Abdulla

  3. Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia

    Ainnul Hamidah Syahadah Azizan, A. Hamid A. Hadi & Kamal Aziz Ketuly

  4. Medical Research Centre, Jazan University, P.O. Box 114, Jazan, Saudi Arabia

    Siddig Ibrahim Abdelwahab & Manal Mohamed Elhassan Taha

  5. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Mal

et al. Retraction Note: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity.BMC Complement Med Ther 24, 166 (2024). https://doi.org/10.1186/s12906-024-04475-5Download citationPublished: 19 April 2024DOI: https://doi.org/10.1186/s12906-024-04475-5Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative.
{"title":"Retraction Note: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity","authors":"Heyam Mohamed Ali Sidahmed, Ainnul Hamidah Syahadah Azizan, Syam Mohan, Mahmood Ameen Abdulla, Siddig Ibrahim Abdelwahab, Manal Mohamed Elhassan Taha, A. Hamid A. Hadi, Kamal Aziz Ketuly, Najihah Mohd Hashim, Mun Fai Loke, Jamuna Vadivelu","doi":"10.1186/s12906-024-04475-5","DOIUrl":"https://doi.org/10.1186/s12906-024-04475-5","url":null,"abstract":"<p><b>Retraction Note: BMC Complement Med Ther 13, 183 (2013)</b></p><p><b>https://doi.org/10.1186/1472-6882-13-183</b></p><p>The Editors retracted this article because of concerns regarding a number of figures presented in this work. These concerns call into question the article’s overall scientific soundness and its authorship. An investigation conducted after its publication discovered similarities between images in this work and images in [1] and [2],</p><ul>\u0000<li>\u0000<p>Panel D in Fig. 5 and panel B in Fig. 5 in [1];</p>\u0000</li>\u0000<li>\u0000<p>Panel D in Fig. 6 and panel E in Fig. 5 in [2];</p>\u0000</li>\u0000<li>\u0000<p>Panel F in Fig. 5 and panel E in Fig. 4 in [1];</p>\u0000</li>\u0000<li>\u0000<p>The bottom part of panel F in Fig. 5 and the top part of panel E in Fig. 4 in [2];</p>\u0000</li>\u0000</ul><p>The Editors therefore no longer have confidence in the integrity of the research presented in this article. Jamuna Vadivelu and Mun Fai Loke agree with the retraction. The remaining authors have not responded to correspondence from the Publisher about the retraction.</p><ol data-track-component=\"outbound reference\"><li data-counter=\"1.\"><p>Al Batran R, Al-Bayaty F, Ameen Abdulla M, Al-Obaidi J, Hajrezaei MM, Hassandarvish M, Fouad P, Golbabapour M, S. and, Talaee S. Gastroprotective effects of <i>Corchorus olitorius</i>. J Gastroenterol Hepatol. 2013;28:1321–9. https://doi.org/10.1111/jgh.12229.</p><p>Article PubMed PubMed Central Google Scholar </p></li><li data-counter=\"2.\"><p>Ismail IF, Golbabapour S, Hassandarvish P, Hajrezaie M, Majid NA, Kadir FA. Fouad Al-Bayaty, Khalijah Awang, Hazrina Hazni, Mahmood Ameen Abdulla, Gastroprotective Activity of <i>Polygonum chinense</i> Aqueous Leaf Extract on Ethanol-Induced Hemorrhagic Mucosal Lesions in Rats, <i>Evidence-Based Complementary and Alternative Medicine</i>, vol. 2012, Article ID 404012, 9 pages, 2012. https://doi.org/10.1155/2012/404012.</p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><span>Author notes</span><ol><li><p>Equal contributors.</p></li></ol><h3>Authors and Affiliations</h3><ol><li><p>Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia</p><p>Heyam Mohamed Ali Sidahmed, Syam Mohan &amp; Najihah Mohd Hashim</p></li><li><p>Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia</p><p>Mahmood Ameen Abdulla</p></li><li><p>Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia</p><p>Ainnul Hamidah Syahadah Azizan, A. Hamid A. Hadi &amp; Kamal Aziz Ketuly</p></li><li><p>Medical Research Centre, Jazan University, P.O. Box 114, Jazan, Saudi Arabia</p><p>Siddig Ibrahim Abdelwahab &amp; Manal Mohamed Elhassan Taha</p></li><li><p>Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Mal","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative effectiveness of Propolis with chlorhexidine mouthwash on gingivitis – a randomized controlled clinical study 蜂胶与洗必泰漱口水对牙龈炎的疗效比较--随机对照临床研究
2区 医学 Q1 Medicine Pub Date : 2024-04-06 DOI: 10.1186/s12906-024-04456-8
Shilpa Gunjal, Deepak Gowda Sadashivappa Pateel
To assess and compare the effectiveness of propolis mouthwash with chlorhexidine mouthwash in the reduction of plaque and gingivitis. A single centre, latin-square cross-over, double masked, randomized controlled clinical trial was conducted on 45 chronic generalized gingivitis subjects who were chosen from the dental clinic of MAHSA University, Malaysia. A total of 45 subjects were randomly assigned into one of the three different groups (n = 15 each) using a computer-generated random allocation sequence: Group A Propolis mouthwash; Group B Chlorhexidine mouthwash; and Group C Placebo mouthwash. Supragingival plaque and gingival inflammation were assessed by full mouth Plaque index (PI) and gingival index (GI) at baseline and after 21 days. The study was divided into three phases, each phase lasted for 21 days separated by a washout period of 15 days in between them. Groups A, B and C were treated with 0.2% Propolis, Chlorhexidine, and Placebo mouthwash, respectively, in phase I. The study subjects were instructed to use the assigned mouthwash twice daily for 1 min for 21 days. On day 22nd, the subjects were recalled for measurement of PI and GI. After phase I, mouthwash was crossed over as dictated by the Latin square design in phase II and III. At baseline, intergroup comparison revealed no statistically significant difference between Groups A, B and C (p > 0.05). On day 21, one-way ANOVA revealed statistically significant difference between the three groups for PI (p < 0.001) and GI (p < 0.001). Bonferroni post-hoc test showed statistically significant difference between Propolis and Chlorhexidine mouthwash (P < 0.001), with higher reduction in the mean plaque and gingival scores in propolis group compared to chlorhexidine and placebo groups. Propolis mouthwash demonstrated significant improvement in gingival health and plaque reduction. Thus, it could be used as an effective herbal mouthwash alternative to chlorhexidine mouthwash. The trial was retrospectively registered on 25/07/2019 at clinicaltrials.gov and its identifier is NCT04032548.
评估和比较蜂胶漱口水与洗必泰漱口水在减少牙菌斑和牙龈炎方面的效果。我们在马来西亚 MAHSA 大学的牙科诊所对 45 名慢性牙龈炎患者进行了单中心、拉丁方形交叉、双面具随机对照临床试验。通过计算机生成的随机分配序列,45 名受试者被随机分配到三个不同的组别中(每组 15 人):A 组蜂胶漱口水;B 组洗必泰漱口水;C 组安慰剂漱口水。在基线和 21 天后,通过全口牙菌斑指数 (PI) 和牙龈指数 (GI) 评估龈上牙菌斑和牙龈炎症。研究分为三个阶段,每个阶段持续 21 天,中间有 15 天的冲洗期。A 组、B 组和 C 组在第一阶段分别使用 0.2% 蜂胶漱口水、洗必泰漱口水和安慰剂漱口水。第 22 天,再次召集受试者测量 PI 和 GI。第一阶段结束后,根据第二和第三阶段的拉丁方形设计,漱口水被交叉使用。基线时,组间比较显示 A 组、B 组和 C 组之间没有明显的统计学差异(P > 0.05)。第 21 天,单因素方差分析显示,三组的 PI(P < 0.001)和 GI(P < 0.001)差异有统计学意义。Bonferroni 事后检验显示,蜂胶漱口水和洗必泰漱口水之间的差异有统计学意义(P < 0.001),蜂胶组的牙菌斑和牙龈平均得分比洗必泰组和安慰剂组降低得更多。蜂胶漱口水明显改善了牙龈健康,减少了牙菌斑。因此,蜂胶漱口水可作为洗必泰漱口水的有效草药漱口水替代品。该试验于2019年7月25日在clinicaltrials.gov进行了回顾性注册,其标识符为NCT04032548。
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引用次数: 0
Evaluation of Lippia scaberrima Sond. and Aspalathus linearis (Burm.f.) R. Dahlgren extracts on human CYP enzymes and gold nanoparticle synthesis: implications for drug metabolism and cytotoxicity Lippia scaberrima Sond.和 Aspalathus linearis (Burm.f.) R. Dahlgren 提取物对人体 CYP 酶和金纳米粒子合成的影响:对药物代谢和细胞毒性的影响
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04439-9
Anna-Mari Kok, Risto Juvonen, Markku Pasanen, Vusani Mandiwana, Michel Lonji Kalombo, Suprakas Sinha Ray, Rirhandzu Rikhotso-Mbungela, Namrita Lall
Metabolism is an important component of the kinetic characteristics of herbal constituents, and it often determines the internal dose and concentration of these effective constituents at the target site. The metabolic profile of plant extracts and pure compounds need to be determined for any possible herb-drug metabolic interactions that might occur. Various concentrations of the essential oil of Lippia scaberrima, the ethanolic extract of Lippia scaberrima alone and their combinations with fermented and unfermented Aspalathus linearis extract were used to determine the inhibitory potential on placental, microsomal and recombinant human hepatic Cytochrome P450 enzymes. Furthermore, the study investigated the synthesis and characterization of gold nanoparticles from the ethanolic extract of Lippia scaberrima as a lead sample. Confirmation and characterization of the synthesized gold nanoparticles were conducted through various methods. Additionally, the cytotoxic properties of the ethanolic extract of Lippia scaberrima were compared with the gold nanoparticles synthesized from Lippia scaberrima using gum arabic as a capping agent. All the samples showed varying levels of CYP inhibition. The most potent inhibition took place for CYP2C19 and CYP1B1 with 50% inhibitory concentration (IC50) values of less than 0.05 µg/L for the essential oil tested and IC50-values between 0.05 µg/L-1 µg/L for all the other combinations and extracts tested, respectively. For both CYP1A2 and CYP2D6 the IC50-values for the essential oil, the extracts and combinations were found in the range of 1 – 10 µg/L. The majority of the IC50 values found were higher than 10 µg/L and, therefore, were found to have no inhibition against the CYP enzymes tested. Therefore, the essential oil of Lippia scaberrima, the ethanolic extract of Lippia scaberrima alone and their combinations with Aspalathus linearis do not possess any clinically significant CYP interaction potential and may be further investigated for their adjuvant potential for use in the tuberculosis treatment regimen. Furthermore, it was shown that the cytotoxic potential of the Lippia scaberrima gold nanoparticles was reduced by twofold when compared to the ethanolic extract of Lippia scaberrima.
新陈代谢是草药成分动力学特性的重要组成部分,它往往决定了这些有效成分在目标部位的内部剂量和浓度。需要确定植物提取物和纯化合物的代谢概况,以了解可能发生的草药-药物代谢相互作用。本研究使用了不同浓度的 Lippia scaberrima 精油、单独的 Lippia scaberrima 乙醇提取物及其与发酵和未发酵 Aspalathus linearis 提取物的组合,以确定其对胎盘、微粒体和重组人肝脏细胞色素 P450 酶的抑制潜力。此外,该研究还调查了以 Lippia scaberrima 的乙醇提取物为先导样品的金纳米粒子的合成和表征。通过各种方法对合成的金纳米粒子进行了确认和表征。此外,还比较了葶苈子乙醇提取物与以阿拉伯树胶为封盖剂从葶苈子中合成的金纳米粒子的细胞毒性特性。所有样品都表现出不同程度的 CYP 抑制作用。对 CYP2C19 和 CYP1B1 的抑制作用最强,所测精油的 50%抑制浓度 (IC50) 值小于 0.05 µg/L,而所有其他组合和所测提取物的 IC50 值均在 0.05 µg/L-1 µg/L 之间。对于 CYP1A2 和 CYP2D6,精油、提取物和组合的 IC50 值在 1 - 10 µg/L 之间。所发现的 IC50 值大多高于 10 µg/L,因此对所测试的 CYP 酶没有抑制作用。因此,Lippia scaberrima 的精油、Lippia scaberrima 的乙醇提取物本身以及它们与 Aspalathus linearis 的组合在临床上并不具有任何显著的 CYP 相互作用潜力,可以进一步研究它们在结核病治疗方案中的辅助潜力。此外,研究还表明,与葶苈子乙醇提取物相比,葶苈子金纳米粒子的细胞毒性降低了两倍。
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引用次数: 0
Epigallocatechin-3-Gallate attenuates lipopolysacharide-induced pneumonia via modification of inflammation, oxidative stress, apoptosis, and autophagy 表没食子儿茶素-3-棓酸盐通过改变炎症、氧化应激、细胞凋亡和自噬,减轻脂多巴胂诱发的肺炎
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04436-y
Meili Shen, Yuting You, Chengna Xu, Zhixu Chen
Pneumonia, the acute inflammation of lung tissue, is multi-factorial in etiology. Hence, continuous studies are conducted to determine the mechanisms involved in the progression of the disease and subsequently suggest effective treatment. The present study attempted to evaluate the effects of Epigallocatechin-3-Gallate (EGCG), an herbal antioxidant, on inflammation, oxidative stress, apoptosis, and autophagy in a rat pneumonia model. Forty male Wistar rats, 5 months old and 250–290 g were divided into four groups including control, EGCG, experimental pneumonia (i/p LPS injection, 1 mg/kg), and experimental pneumonia treated with EGCG (i/p, 15 mg/kg, 1 h before and 3 h after LPS instillation). Total cell number in the bronchoalveolar lavage fluid, inflammation (TNF-a, Il-6, IL-1β, and NO), oxidative stress (Nrf2, HO-1, SOD, CAT, GSH, GPX, MDA, and TAC), apoptosis (BCL-2, BAX, CASP-3 and CASP-9), and autophagy (mTOR, LC3, BECN1) were evaluated. The findings demonstrated that EGCG suppresses the LPS-induced activation of inflammatory pathways by a significant reduction of inflammatory markers (p-value < 0.001). In addition, the upregulation of BCL-2 and downregulation of BAX and caspases revealed that EGCG suppressed LPS-induced apoptosis. Furthermore, ECGC suppressed oxidative injury while promoting autophagy in rats with pneumonia (p-value < 0.05). The current study revealed that EGCG could suppress inflammation, oxidative stress, apoptosis, and promote autophagy in experimental pneumonia models of rats suggesting promising therapeutical properties of this compound to be used in pneumonia management.
肺炎是肺组织的急性炎症,其病因是多因素的。因此,人们不断进行研究,以确定疾病发展的相关机制,进而提出有效的治疗建议。本研究试图评估表没食子儿茶素-3-棓酸盐(EGCG)(一种草本抗氧化剂)对大鼠肺炎模型中炎症、氧化应激、细胞凋亡和自噬的影响。将40只体重250-290克、5个月大的雄性Wistar大鼠分为四组,包括对照组、EGCG组、实验性肺炎组(LPS注射,1毫克/千克,i/p)和EGCG治疗实验性肺炎组(LPS注射前1小时和注射后3小时,i/p,15毫克/千克)。对支气管肺泡灌洗液中的细胞总数、炎症(TNF-a、Il-6、IL-1β和NO)、氧化应激(Nrf2、HO-1、SOD、CAT、GSH、GPX、MDA和TAC)、细胞凋亡(BCL-2、BAX、CASP-3和CASP-9)和自噬(mTOR、LC3和BECN1)进行了评估。研究结果表明,EGCG 能抑制 LPS 诱导的炎症通路激活,显著降低炎症标志物(p 值 < 0.001)。此外,BCL-2 的上调以及 BAX 和 caspases 的下调表明,EGCG 可抑制 LPS 诱导的细胞凋亡。此外,ECGC 在促进肺炎大鼠自噬的同时还抑制了氧化损伤(p 值 < 0.05)。目前的研究表明,EGCG 可抑制大鼠实验性肺炎模型中的炎症、氧化应激、细胞凋亡并促进自噬,这表明该化合物具有良好的治疗特性,有望用于肺炎的治疗。
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引用次数: 0
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BMC Complementary and Alternative Medicine
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