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Protective effect of Ganoderma lucidum-fermented crop extracts against hydrogen peroxide- or β-amyloid-induced damage in human neuronal SH-SY5Y cells 灵芝发酵作物提取物对过氧化氢或 β 淀粉样蛋白诱导的人神经元 SH-SY5Y 细胞损伤的保护作用
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04409-1
Chung-Hsiung Huang, Yu-Ting Liao, Chien-Li Chen, Guo-Jane Tsai
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the accumulation of stacked β-amyloid peptides in the brain and associated with the generation of oxidative stress. So far, there is no cure for AD or a way to stop its progression. Although the neuroprotective effects of Ganoderma lucidum aqueous extract and G. lucidum-derived triterpenoids and polysaccharides have been reported, the influence of G. lucidum-fermented crops on AD still lacks clarity. This study aimed to investigate the protective effect of G. lucidum-fermented crop extracts against hydrogen peroxide- or β-amyloid peptide (Aβ25-35)-induced damage in human neuroblastoma SH-SY5Y cells. Various extracts of G. lucidum-fermented crops, including extract A: 10% ethanol extraction using microwave, extract B: 70˚C water extraction, and extract C: 100˚C water extraction followed by ethanol precipitation, were prepared and analyzed. Extract B had the highest triterpenoid content. Extract C had the highest total glucan content, while extract A had the highest gamma-aminobutyric acid (GABA) content. The median inhibitory concentration (IC50, mg/g) for DPPH and ABTS scavenging activity of the fermented crop extracts was significantly lower than that of the unfermented extract. Pretreatment with these extracts significantly increased the cell viability of SH-SY5Y cells damaged by H2O2 or Aβ25-35, possibly by reducing cellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels and increasing superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. Moreover, extract B markedly alleviated the activity of acetylcholinesterase (AChE), which is crucial in the pathogenesis of AD. These results clearly confirmed the effects of G. lucidum-fermented crop extracts on preventing against H2O2- or Aβ25-35-induced neuronal cell death and inhibiting AChE activity, revealing their potential in management of AD.
阿尔茨海默病(AD)是一种神经退行性疾病,其特征是大脑中堆积的β-淀粉样肽,并与氧化应激的产生有关。迄今为止,还没有治疗 AD 的方法,也没有阻止其发展的方法。虽然灵芝水提取物以及灵芝衍生的三萜类和多糖具有神经保护作用,但灵芝发酵作物对AD的影响仍不明确。本研究的目的是探讨银耳发酵提取物对过氧化氢或β-淀粉样肽(Aβ25-35)诱导的人神经母细胞瘤SH-SY5Y细胞损伤的保护作用。制备并分析了绿藻发酵作物的各种提取物,包括提取物A:10%乙醇微波提取、提取物B:70˚C水提取和提取物C:100˚C水提取后乙醇沉淀。提取物 B 的三萜类化合物含量最高。提取物 C 的总葡聚糖含量最高,而提取物 A 的γ-氨基丁酸(GABA)含量最高。发酵作物提取物的 DPPH 和 ABTS 清除活性的中位抑制浓度(IC50,毫克/克)明显低于未发酵提取物。用这些提取物预处理可明显提高受 H2O2 或 Aβ25-35 损伤的 SH-SY5Y 细胞的存活率,这可能是通过降低细胞活性氧(ROS)和丙二醛(MDA)水平以及提高超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)活性实现的。此外,提取物 B 还能明显降低乙酰胆碱酯酶(AChE)的活性,而乙酰胆碱酯酶在注意力缺失症的发病机制中起着至关重要的作用。这些结果清楚地证实了绿藻发酵作物提取物在防止H2O2-或Aβ25-35诱导的神经细胞死亡和抑制乙酰胆碱酯酶活性方面的作用,揭示了它们在治疗AD方面的潜力。
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引用次数: 0
The ameliorating effect of Rutin on hepatotoxicity and inflammation induced by the daily administration of vortioxetine in rats 芦丁对大鼠每日服用伏替西汀引起的肝毒性和炎症的改善作用
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04447-9
Mai M. Anwar, Ibrahim M. Ibrahim Laila
Vortioxetine (VORTX) is a potent and selective type of selective serotonin reuptake inhibitor (SSRI) that is mainly prescribed for treating major depression along with mood disorders as the first drug of choice. Limited previous findings have indicated evidence of liver injury and hepatotoxicity associated with daily VORTX treatment. Rutin (RUT), which is known for its antioxidant properties, has demonstrated several beneficial health actions, including hepatoprotection. Therefore the current study aimed to evaluate and assess the ameliorative effect of RUT against the hepatotoxic actions of daily low and high-dose VORTX administration. The experimental design included six groups of rats, each divided equally. Control, rats exposed to RUT (25 mg/kg), rats exposed to VORTX (28 mg/kg), rats exposed to VORTX (28 mg/kg) + RUT (25 mg/kg), rats exposed to VORTX (80 mg/kg), and rats exposed to VORTX (80 mg/kg) + RUT (25 mg/kg). After 30 days from the daily exposure period, assessments were conducted for serum liver enzyme activities, hepatotoxicity biomarkers, liver antioxidant endogenous enzymes, DNA fragmentation, and histopathological studies of liver tissue. Interestingly, the risk of liver damage and hepatotoxicity related to VORTX was attenuated by the daily co-administration of RUT. Significant improvements were observed among all detected liver functions, oxidative stress, and inflammatory biomarkers including aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), albumin, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), glutathione S-transferase (GST), total protein, acid phosphatase, N-Acetyl-/β-glucosaminidase (β-NAG), β-Galactosidase (β-Gal), alpha-fetoprotein (AFP), caspase 3, and cytochrom-C along with histopathological studies, compared to the control and sole RUT group. Thus, RUT can be considered a potential and effective complementary therapy in preventing hepatotoxicity and liver injury induced by the daily or prolonged administration of VORTX.
伏替西汀(VORTX)是一种强效的选择性血清素再摄取抑制剂(SSRI),主要用于治疗重度抑郁症和情绪障碍,是首选药物。以往有限的研究结果表明,每天服用 VORTX 会导致肝损伤和肝毒性。芦丁(RUT)以其抗氧化特性而闻名,已被证明具有多种有益健康的作用,包括保护肝脏。因此,本研究旨在评价和评估芦丁对每天服用低剂量和高剂量 VORTX 所引起的肝毒性作用的改善效果。实验设计包括六组大鼠,每组平均分配。对照组、暴露于 RUT(25 毫克/千克)的大鼠组、暴露于 VORTX(28 毫克/千克)的大鼠组、暴露于 VORTX(28 毫克/千克)+ RUT(25 毫克/千克)的大鼠组、暴露于 VORTX(80 毫克/千克)的大鼠组和暴露于 VORTX(80 毫克/千克)+ RUT(25 毫克/千克)的大鼠组。每天暴露 30 天后,对血清肝酶活性、肝毒性生物标志物、肝脏抗氧化内源酶、DNA 断裂进行评估,并对肝组织进行组织病理学研究。有趣的是,每天同时服用 RUT 可降低与 VORTX 有关的肝损伤和肝毒性风险。所有检测到的肝功能、氧化应激和炎症生物标志物均有显著改善,包括天门冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)、白蛋白、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷氨酰转肽酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽(GSH)、谷胱甘肽(GSH)和谷胱甘肽(GSH)、总蛋白、酸性磷酸酶、N-乙酰基-/β-氨基葡萄糖苷酶(β-NAG)、β-半乳糖苷酶(β-Gal)、甲胎蛋白(AFP)、Caspase 3 和细胞色素-C,以及组织病理学研究。因此,RUT 可被视为一种潜在而有效的辅助疗法,可预防每日或长期服用 VORTX 引起的肝毒性和肝损伤。
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引用次数: 0
Mechanism of Smilax china L. in the treatment of intrauterine adhesions based on network pharmacology, molecular docking and experimental validation 基于网络药理学、分子对接和实验验证的Smilax china L.治疗宫腔内粘连的机制
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04414-4
Tingting Shi, Chuqi Hou, Yongzhen Duan, Yuliang Li, Wenqin Liu, Peixian Huang, Yuhua Zhou, Shanshan Yu, Luyao Song
Smilax china L. (SCL) is a traditional herbal medicine for the potential treatment of intrauterine adhesion (IUA). However, the mechanisms of action have not yet been determined. In this study, we explored the effects and mechanisms of SCL in IUA by network pharmacology, molecular docking and molecular biology experiments. Active ingredients and targets of SCL were acquired from TCMSP and SwissTargetPrediction. IUA-related targets were collected from the GeneCards, DisGeNET, OMIM and TTD databases. A protein‒protein interaction (PPI) network was constructed by Cytoscape 3.9.1 and analysed with CytoHubba and CytoNCA to identify the core targets. The DAVID tool was used for GO and KEGG enrichment analyses. Furthermore, molecular docking was employed to assess the interaction between the compounds and key targets. Finally, the mechanisms and targets of SCL in IUA were verified by cellular experiments and western blot. A total of 196 targets of SCL were identified, among which 93 were related to IUA. Topological and KEGG analyses results identified 15 core targets that were involved in multiple pathways, such as inflammation, apoptosis, and PI3K/AKT signalling pathways. Molecular docking results showed that the active compounds had good binding to the core targets. In vitro experiments showed that astilbin (AST), a major component of SCL, significantly reduced TGF-β-induced overexpression of fibronectin (FN), activation of the PI3K/AKT signalling pathway and the expression of downstream factors (NF-κB and BCL2) in human endometrial stromal cells, suggesting that AST ameliorates IUA by mediating the PI3K/AKT/NF-κB and BCL2 proteins. AST, a major component of SCL, may be a potential therapeutic agent for IUA. Moreover, its mechanism is strongly associated with regulation of the PI3K/AKT signalling pathway and the downstream NF-κB and BCL2 proteins. This study will provide new strategies that utilize AST for the treatment of IUA.
Smilax china L.(SCL)是一种传统草药,可用于治疗宫腔内粘连(IUA)。然而,其作用机制尚未确定。在本研究中,我们通过网络药理学、分子对接和分子生物学实验探索了SCL对IUA的作用和机制。SCL 的有效成分和靶点来自 TCMSP 和 SwissTargetPrediction。IUA相关靶标来自GeneCards、DisGeNET、OMIM和TTD数据库。用 Cytoscape 3.9.1 构建了蛋白质-蛋白质相互作用(PPI)网络,并用 CytoHubba 和 CytoNCA 进行分析,以确定核心靶标。DAVID 工具用于 GO 和 KEGG 富集分析。此外,还采用了分子对接技术来评估化合物与关键靶点之间的相互作用。最后,通过细胞实验和Western blot验证了SCL在IUA中的作用机制和靶点。共鉴定出196个SCL靶点,其中93个与IUA相关。拓扑和KEGG分析结果表明,有15个核心靶点涉及多个通路,如炎症、细胞凋亡和PI3K/AKT信号通路。分子对接结果显示,活性化合物与核心靶点结合良好。体外实验表明,SCL的主要成分Astilbin(AST)能显著降低TGF-β诱导的人子宫内膜基质细胞中纤维连接蛋白(FN)的过表达、PI3K/AKT信号通路的激活以及下游因子(NF-κB和BCL2)的表达,这表明AST能通过介导PI3K/AKT/NF-κB和BCL2蛋白来改善IUA。作为 SCL 的主要成分,AST 可能是 IUA 的潜在治疗药物。此外,其机制与 PI3K/AKT 信号通路及下游 NF-κB 和 BCL2 蛋白的调节密切相关。这项研究将为利用 AST 治疗 IUA 提供新的策略。
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引用次数: 0
Effects of garlic (Allium sativum L) and Citrullus colocynthis (L.) Schrad individually and in combination on male reproductive damage due to diabetes: suppression of the AGEs/RAGE/Nox-4 signaling pathway 大蒜(Allium sativum L)和壳斗(Citrullus colocynthis (L.) Schrad)单独或混合使用对糖尿病导致的男性生殖损伤的影响:抑制 AGEs/RAGE/Nox-4 信号通路
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04402-8
Aghileh Mohammadzadeh, Ali Gol, Reza Kheirandish
Diabetes Mellitus is associated with disturbances in male reproductive function and fertility. Studies have shown that oxidative stress with the subsequent inflammation and apoptosis cause these complications in diabetes. Garlic (G) (Allium sativum L) and Citrullus colocynthis (L.) Schrad (C) both have antidiabetic and antioxidant properties. Recently, we demonstrated their synergistic effects in alleviating reproductive complications when administered concomitantly. However, as even medicinal plants in long term usage may lead to some unwanted side effects of their own, we examined whether with half the original doses of these two medicinal plants we could achieve the desired results. Thirty-five male Wistar rats were divided into five groups (n = 7/group): Control, Diabetic, Diabetic + G (0.5 ml/100 g BW), Diabetic + C (5 mg/kg BW) and Diabetic + GC (0.5 ml/100 g BW of garlic and 5 mg/kg BW of C. colocynthis) groups. The experimental period was 30 days. Oxidative stress, advanced glycation end products (AGEs), immunoexpression of caspase-3, and expression of mRNAs for receptor for advanced glycation end products (RAGE), NADPH oxidase-4 (NOX-4) and nuclear factor kappa B increased in testis of diabetic rats. Treatment with garlic and C. colocynthis alone showed some beneficial effects, but in the combination form the effectiveness was more profound. We conclude that the combination therapy of diabetic rats with lower doses is still as efficient as higher doses; therefore, the way forward for reducing complications in long term consumption.
糖尿病与男性生殖功能和生育能力紊乱有关。研究表明,氧化应激以及随之而来的炎症和细胞凋亡是导致糖尿病并发症的原因。大蒜(G)(Allium sativum L)和壳斗(Citrullus colocynthis (L.) Schrad (C))都具有抗糖尿病和抗氧化特性。最近,我们证实了同时服用这两种药物在缓解生殖系统并发症方面的协同作用。然而,即使是长期使用的药用植物也可能会导致一些不必要的副作用,因此我们研究了将这两种药用植物的剂量减半是否能达到预期效果。35 只雄性 Wistar 大鼠被分为五组(n = 7/组):对照组、糖尿病组、糖尿病 + G(0.5 毫升/100 克体重)组、糖尿病 + C(5 毫克/千克体重)组和糖尿病 + GC(0.5 毫升/100 克体重的大蒜和 5 毫克/千克体重的款冬花)组。实验周期为 30 天。糖尿病大鼠睾丸中的氧化应激、高级糖化终产物(AGEs)、Caspase-3的免疫表达、高级糖化终产物受体(RAGE)、NADPH氧化酶-4(NOX-4)和核因子卡巴B的mRNA表达均有所增加。单独使用大蒜和秋水仙碱治疗有一定的益处,但联合使用效果更显著。我们得出的结论是,对糖尿病大鼠进行低剂量联合治疗与高剂量治疗一样有效;因此,这是长期服用大蒜减少并发症的未来之路。
{"title":"Effects of garlic (Allium sativum L) and Citrullus colocynthis (L.) Schrad individually and in combination on male reproductive damage due to diabetes: suppression of the AGEs/RAGE/Nox-4 signaling pathway","authors":"Aghileh Mohammadzadeh, Ali Gol, Reza Kheirandish","doi":"10.1186/s12906-024-04402-8","DOIUrl":"https://doi.org/10.1186/s12906-024-04402-8","url":null,"abstract":"Diabetes Mellitus is associated with disturbances in male reproductive function and fertility. Studies have shown that oxidative stress with the subsequent inflammation and apoptosis cause these complications in diabetes. Garlic (G) (Allium sativum L) and Citrullus colocynthis (L.) Schrad (C) both have antidiabetic and antioxidant properties. Recently, we demonstrated their synergistic effects in alleviating reproductive complications when administered concomitantly. However, as even medicinal plants in long term usage may lead to some unwanted side effects of their own, we examined whether with half the original doses of these two medicinal plants we could achieve the desired results. Thirty-five male Wistar rats were divided into five groups (n = 7/group): Control, Diabetic, Diabetic + G (0.5 ml/100 g BW), Diabetic + C (5 mg/kg BW) and Diabetic + GC (0.5 ml/100 g BW of garlic and 5 mg/kg BW of C. colocynthis) groups. The experimental period was 30 days. Oxidative stress, advanced glycation end products (AGEs), immunoexpression of caspase-3, and expression of mRNAs for receptor for advanced glycation end products (RAGE), NADPH oxidase-4 (NOX-4) and nuclear factor kappa B increased in testis of diabetic rats. Treatment with garlic and C. colocynthis alone showed some beneficial effects, but in the combination form the effectiveness was more profound. We conclude that the combination therapy of diabetic rats with lower doses is still as efficient as higher doses; therefore, the way forward for reducing complications in long term consumption.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A systematic review and meta-analysis on sodium tanshinone IIA sulfonate injection for the adjunctive therapy of pulmonary heart disease 关于丹参酮 IIA 磺酸钠注射液辅助治疗肺心病的系统综述和荟萃分析
2区 医学 Q1 Medicine Pub Date : 2024-04-05 DOI: 10.1186/s12906-024-04434-0
Huikai Shao, Fei Yu, Dongsheng Xu, Chunyan Fang, Rongsheng Tong, Lingguo Zhao
Sodium tanshinone IIA sulfonate (STS) injection has been widely used as adjunctive therapy for pulmonary heart disease (PHD) in China. Nevertheless, the efficacy of STS injection has not been systematically evaluated so far. Hence, the efficacy of STS injection as adjunctive therapy for PHD was explored in this study. Randomized controlled trials (RCTs) were screened from China Science and Technology Journal Database, China National Knowledge Infrastructure, Wanfang Database, PubMed, Sino-Med, Google Scholar, Medline, Chinese Biomedical Literature Database, Cochrane Library, Embase and Chinese Science Citation Database until 20 January 2024. Literature searching, data collection and quality assessment were independently performed by two investigators. The extracted data was analyzed with RevMan 5.4 and STATA 14.0. Basing on the methodological quality, dosage of STS injection, control group measures and intervention time, sensitivity analysis and subgroup analysis were performed. 19 RCTs with 1739 patients were included in this study. Results showed that as adjunctive therapy, STS injection combined with Western medicine showed better therapeutic efficacy than Western medicine alone for PHD by increasing the clinical effective rate (RR = 1.22; 95% CI, 1.17 to 1.27; p < 0.001), partial pressure of oxygen (MD = 10.16; 95% CI, 5.07 to 15.24; p < 0.001), left ventricular ejection fraction (MD = 8.66; 95% CI, 6.14 to 11.18; p < 0.001) and stroke volume (MD = 13.10; 95% CI, 11.83 to 14.38; p < 0.001), meanwhile decreasing the low shear blood viscosity (MD = -1.16; 95% CI, -1.57 to -0.74; p < 0.001), high shear blood viscosity (MD = -0.64; 95% CI, -0.86 to -0.42; p < 0.001), plasma viscosity (MD = -0.23; 95% CI, -0.30 to -0.17; p < 0.001), hematokrit (MD = -8.52; 95% CI, -11.06 to -5.98; p < 0.001), fibrinogen (MD = -0.62; 95% CI, -0.87 to -0.37; p < 0.001) and partial pressure of carbon dioxide (MD = -8.56; 95% CI, -12.09 to -5.02; p < 0.001). STS injection as adjunctive therapy seemed to be more effective than Western medicine alone for PHD. However, due to low quality of the included RCTs, more well-designed RCTs were necessary to verify the efficacy of STS injection.
在中国,丹参酮 IIA 磺酸钠注射液(STS)已被广泛用于肺心病(PHD)的辅助治疗。然而,迄今为止,尚未对注射用丹参酮 IIA 磺酸钠的疗效进行系统评估。因此,本研究探讨了 STS 注射液作为 PHD 辅助疗法的疗效。本研究从中国科技期刊数据库、中国国家知识基础设施、万方数据库、PubMed、Sino-Med、Google Scholar、Medline、中国生物医学文献数据库、Cochrane图书馆、Embase和中国科学引文数据库(截至2024年1月20日)中筛选随机对照试验(RCT)。文献检索、数据收集和质量评估由两名研究人员独立完成。提取的数据使用 RevMan 5.4 和 STATA 14.0 进行分析。根据方法学质量、STS注射剂量、对照组措施和干预时间,进行了敏感性分析和亚组分析。本研究共纳入了 19 项 RCT,1739 名患者。结果显示,作为辅助疗法,STS注射液联合西药治疗PHD的临床有效率(RR = 1.22; 95% CI, 1.17 to 1.27; p < 0.001)、氧分压(MD = 10.16; 95% CI, 5.07 to 15.24; p < 0.001)、左室射血分数(MD = 8.66; 95% CI, 6.14 to 11.18; p < 0.001)和搏出量(MD = 13.10; 95% CI, 11.83 to 14.38; p < 0.001),同时降低低剪切血液粘度(MD = -1.16; 95% CI, -1.57 to -0.74; p < 0.001)、高剪切血液粘度(MD = -0.64; 95% CI, -0.86 to -0.42; p < 0.001)、血浆粘度(MD = -0.23; 95% CI, -0.30 to -0.17;p < 0.001)、血细胞比容(MD = -8.52;95% CI,-11.06 to -5.98;p < 0.001)、纤维蛋白原(MD = -0.62;95% CI,-0.87 to -0.37;p < 0.001)和二氧化碳分压(MD = -8.56;95% CI,-12.09 to -5.02;p < 0.001)。注射 STS 作为辅助疗法治疗 PHD 似乎比单独使用西药更有效。然而,由于纳入的研究性临床试验的质量较低,因此需要更多设计良好的研究性临床试验来验证 STS 注射剂的疗效。
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引用次数: 0
Ginger inhibits the invasion of ovarian cancer cells SKOV3 through CLDN7, CLDN11 and CD274 m6A methylation modifications 生姜通过 CLDN7、CLDN11 和 CD274 m6A 甲基化修饰抑制卵巢癌细胞 SKOV3 的侵袭
2区 医学 Q1 Medicine Pub Date : 2024-04-04 DOI: 10.1186/s12906-024-04431-3
Xiaoyu Zhang, Hairong Zhang, Lin Zhu, Lei Xia
Ginger is a common aromatic vegetable with a wide range of functional ingredients and considerable medicinal and nutritional properties. Numerous studies have shown that ginger and its active ingredients have suppressive effects on manifold tumours, including ovarian cancer (OC). However, the molecular mechanism by which ginger inhibits OC is not clear. The aim of this study was to investigate the function and mechanism of ginger in OC. The estimation of n6-methyladenosine (m6A) levels was performed using the m6A RNA Methylation Quantification Kit, and RT-qPCR was used to determine the expression of m6A-related genes and proteins. The m6A methylationome was detected by MeRIP-seq, following analysis of the data. Differential methylation of genes was assessed utilizing RT-qPCR and Western Blotting. The effect of ginger on SKOV3 invasion in ovarian cancer cells was investigated using the wound healing assay and transwell assays. Ginger significantly reduced the m6A level of OC cells SKOV3. The 3’UTR region is the major site of modification for m6A methylation, and its key molecular activities include Cell Adhesion Molecules, according to meRIP-seq results. Moreover, it was observed that Ginger aids significantly in downregulating the CLDN7, CLDN11 mRNA, and protein expression. The results of wound healing assay and transwell assay showed that ginger significantly inhibited the invasion of OC cells SKOV3. Ginger inhibits ovarian cancer cells’ SKOV3 invasion by regulating m6A methylation through CLDN7, CLDN11, and CD274.
生姜是一种常见的芳香蔬菜,具有广泛的功能成分和可观的药用和营养特性。大量研究表明,生姜及其活性成分对包括卵巢癌(OC)在内的多种肿瘤具有抑制作用。然而,生姜抑制卵巢癌的分子机制尚不清楚。本研究旨在探讨生姜在卵巢癌中的作用和机制。使用 m6A RNA 甲基化定量试剂盒估算 n6-甲基腺苷(m6A)水平,并使用 RT-qPCR 测定 m6A 相关基因和蛋白质的表达。在对数据进行分析后,通过 MeRIP-seq 检测了 m6A 甲基化组。利用 RT-qPCR 和 Western 印迹技术评估了基因的甲基化差异。利用伤口愈合试验和透孔试验研究了生姜对卵巢癌细胞 SKOV3 侵袭的影响。生姜能明显降低卵巢癌细胞 SKOV3 的 m6A 水平。根据 meRIP-seq 结果,3'UTR 区域是 m6A 甲基化的主要修饰位点,其关键分子活性包括细胞粘附分子。此外,研究还发现生姜能显著下调 CLDN7、CLDN11 mRNA 和蛋白质的表达。伤口愈合试验和透孔试验结果表明,生姜能显著抑制卵巢癌细胞 SKOV3 的侵袭。生姜通过CLDN7、CLDN11和CD274调节m6A甲基化,从而抑制卵巢癌细胞SKOV3的侵袭。
{"title":"Ginger inhibits the invasion of ovarian cancer cells SKOV3 through CLDN7, CLDN11 and CD274 m6A methylation modifications","authors":"Xiaoyu Zhang, Hairong Zhang, Lin Zhu, Lei Xia","doi":"10.1186/s12906-024-04431-3","DOIUrl":"https://doi.org/10.1186/s12906-024-04431-3","url":null,"abstract":"Ginger is a common aromatic vegetable with a wide range of functional ingredients and considerable medicinal and nutritional properties. Numerous studies have shown that ginger and its active ingredients have suppressive effects on manifold tumours, including ovarian cancer (OC). However, the molecular mechanism by which ginger inhibits OC is not clear. The aim of this study was to investigate the function and mechanism of ginger in OC. The estimation of n6-methyladenosine (m6A) levels was performed using the m6A RNA Methylation Quantification Kit, and RT-qPCR was used to determine the expression of m6A-related genes and proteins. The m6A methylationome was detected by MeRIP-seq, following analysis of the data. Differential methylation of genes was assessed utilizing RT-qPCR and Western Blotting. The effect of ginger on SKOV3 invasion in ovarian cancer cells was investigated using the wound healing assay and transwell assays. Ginger significantly reduced the m6A level of OC cells SKOV3. The 3’UTR region is the major site of modification for m6A methylation, and its key molecular activities include Cell Adhesion Molecules, according to meRIP-seq results. Moreover, it was observed that Ginger aids significantly in downregulating the CLDN7, CLDN11 mRNA, and protein expression. The results of wound healing assay and transwell assay showed that ginger significantly inhibited the invasion of OC cells SKOV3. Ginger inhibits ovarian cancer cells’ SKOV3 invasion by regulating m6A methylation through CLDN7, CLDN11, and CD274.","PeriodicalId":9132,"journal":{"name":"BMC Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroprotective effects of methanolic extract from Chuanxiong Rhizoma in mice with middle cerebral artery occlusion-induced ischemic stroke: suppression of astrocyte- and microglia-related inflammatory response 川芎甲醇提取物对大脑中动脉闭塞所致缺血性脑卒中小鼠的神经保护作用:抑制星形胶质细胞和小胶质细胞相关炎症反应
2区 医学 Q1 Medicine Pub Date : 2024-04-04 DOI: 10.1186/s12906-024-04454-w
Chiyeon Lim, Sehyun Lim, So-Jung Moon, Suin Cho
In traditional Asian medicine, dried rhizomes of Ligusticum chuanxiong Hort. (Chuanxiong Rhizoma [CR]) have long been used to treat pain disorders that affect the head and face such as headaches. Furthermore, they have been used primarily for blood circulation improvement or as an analgesic and anti-inflammatory medicine. This study aimed to investigate the neuroprotective effects of a methanol extract of CR (CRex) on ischemic stroke in mice caused by middle cerebral artery occlusion (MCAO). C57BL/6 mice were given a 1.5-h transient MCAO (MCAO control and CRex groups); CRex was administered in the mice of the CRex group at 1,000–3,000 mg/kg either once (single dose) or twice (twice dose) before MCAO. The mechanism behind the neuroprotective effects of CRex was examined using the following techniques: brain infarction volume, edema, neurological deficit, novel object recognition test (NORT), forepaw grip strength, and immuno-fluorescence staining. Pretreating the mice with CRex once at 1,000 or 3,000 mg/kg and twice at 1,000 mg/kg 1 h before MCAO, brought about a significantly decrease in the infarction volumes. Furthermore, pretreating mice with CRex once at 3,000 mg/kg 1 h before MCAO significantly suppressed the reduction of forepaw grip strength of MCAO-induced mice. In the MCAO-induced group, preadministration of CRex inhibited the reduction in the discrimination ratio brought on by MCAO in a similar manner. CRex exhibited these effects by suppressing the activation of astrocytes and microglia, which regulated the inflammatory response. This study proposes a novel development for the treatment of ischemic stroke and provides evidence favoring the use of L. chuanxiong rhizomes against ischemic stroke.
在亚洲传统医学中,川芎(Chuanxiong Rhizoma [CR])的干燥根茎长期以来一直被用于治疗头面部疼痛,如头痛。(Chuanxiong Rhizoma [CR])的干燥根茎长期以来一直被用于治疗影响头面部的疼痛疾病,如头痛。此外,川芎还主要用于改善血液循环或作为镇痛消炎药。本研究旨在探讨 CR 的甲醇提取物(CRex)对大脑中动脉闭塞(MCAO)引起的小鼠缺血性中风的神经保护作用。对 C57BL/6 小鼠进行 1.5 小时的瞬时 MCAO(MCAO 对照组和 CRex 组);在 MCAO 前,给 CRex 组小鼠注射 CRex,剂量为 1,000-3,000 mg/kg,一次(单剂量)或两次(双剂量)。CRex神经保护作用的机制是通过以下技术进行检测的:脑梗死体积、水肿、神经功能缺损、新物体识别测试(NORT)、前爪握力和免疫荧光染色。在小鼠 MCAO 前 1 小时用 CRex 预处理 1,000 或 3,000 毫克/千克,以及 2 次 1,000 毫克/千克,可显著减少脑梗塞体积。此外,在 MCAO 诱导的小鼠前爪握力下降的情况下,MCAO 诱导的小鼠在 MCAO 前 1 小时预处理一次 CRex(3,000 毫克/千克)可明显抑制其前爪握力的下降。在MCAO诱导组中,预给药CRex也以类似的方式抑制了MCAO导致的辨别率下降。CRex通过抑制调节炎症反应的星形胶质细胞和小胶质细胞的活化发挥了上述作用。本研究提出了治疗缺血性中风的新方法,并提供了使用川芎治疗缺血性中风的有利证据。
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引用次数: 0
Catharanthus roseus intoxication mimicking acute cholangitis 模仿急性胆管炎的长春花中毒症
2区 医学 Q1 Medicine Pub Date : 2024-04-04 DOI: 10.1186/s12906-024-04441-1
Yoen Young Chuah, Yeong Yeh Lee, Chu-Kuang Chou, Li-Jen Chang
Catharanthus roseus, a Madagascar native flowering plant, is known for its glossy leaves and vibrant flowers, and its medicinal significance due to its alkaloid compounds. As a source of vinblastine and vincristine used in chemotherapy, Catharanthus roseus is also employed in traditional medicine with its flower and stalks in dried form. Its toxicity can lead to various adverse effects. We report a case of Catharanthus roseus juice toxicity presenting as acute cholangitis, emphasizing the importance of healthcare providers obtaining detailed herbal supplement histories. A 65-year-old woman presented with abdominal pain, fever, anorexia, and lower limb numbness. Initial diagnosis of acute cholangitis was considered, but imaging excluded common bile duct stones. Further investigation revealed a history of ingesting Catharanthus roseus juice for neck pain. Laboratory findings showed leukocytosis, elevated liver enzymes, and hyperbilirubinemia. The patient developed gastric ulcers, possibly due to alkaloids in Catharanthus roseus. No bacterial growth was noted in blood cultures. The patient recovered after discontinuing the herbal extract. Catharanthus roseus toxicity can manifest as fever, hepatotoxicity with cholestatic jaundice, and gastric ulcers, mimicking acute cholangitis. Awareness of herbal supplement use and potential toxicities is crucial for healthcare providers to ensure prompt diagnosis and appropriate management. This case emphasizes the need for public awareness regarding the possible toxicity of therapeutic herbs and the importance of comprehensive patient histories in healthcare settings.
长春花(Catharanthus roseus)是马达加斯加本土的一种开花植物,以其光洁的叶子和鲜艳的花朵而闻名,其生物碱化合物具有重要的药用价值。作为化疗中使用的长春碱和长春新碱的来源,长春花也以其干花和茎秆被用于传统医药中。其毒性可导致各种不良反应。我们报告了一例以急性胆管炎为表现形式的长春花汁中毒病例,强调了医疗服务提供者获取详细草药补充剂病史的重要性。一名 65 岁的妇女因腹痛、发热、厌食和下肢麻木就诊。初步诊断为急性胆管炎,但影像学检查排除了胆总管结石。进一步检查发现,患者曾因颈部疼痛而摄入蔷薇果汁。实验室检查结果显示白细胞增多、肝酶升高和高胆红素血症。患者出现了胃溃疡,可能是由于水飞蓟中的生物碱引起的。血液培养未发现细菌生长。患者在停用草药提取物后康复。长春花毒性可表现为发热、胆汁淤积性黄疸的肝毒性和胃溃疡,模仿急性胆管炎。医疗服务提供者必须了解草药补充剂的使用和潜在毒性,以确保及时诊断和适当处理。本病例强调了提高公众对治疗性草药可能产生的毒性的认识的必要性,以及在医疗机构全面记录患者病史的重要性。
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引用次数: 0
Cognitive enrichment through art: a randomized controlled trial on the effect of music or visual arts group practice on cognitive and brain development of young children 通过艺术丰富认知:音乐或视觉艺术小组练习对幼儿认知和大脑发育影响的随机对照试验
2区 医学 Q1 Medicine Pub Date : 2024-04-04 DOI: 10.1186/s12906-024-04433-1
C. E. James, M. Tingaud, G. Laera, C. Guedj, S. Zuber, R. Diambrini Palazzo, S. Vukovic, J. Richiardi, M. Kliegel, D. Marie
The optimal stimulation for brain development in the early academic years remains unclear. Current research suggests that musical training has a more profound impact on children's executive functions (EF) compared to other art forms. What is crucially lacking is a large-scale, long-term genuine randomized controlled trial (RCT) in cognitive neuroscience, comparing musical instrumental training (MIP) to another art form, and a control group (CG). This study aims to fill this gap by using machine learning to develop a multivariate model that tracks the interconnected brain and EF development during the academic years, with or without music or other art training. The study plans to enroll 150 children aged 6-8 years and randomly assign them to three groups: Orchestra in Class (OC), Visual Arts (VA), and a control group (CG). Anticipating a 30% attrition rate, each group aims to retain at least 35 participants. The research consists of three analytical stages: 1) baseline analysis correlating EF, brain data, age, gender, and socioeconomic status, 2) comparison between groups and over time of EF brain and behavioral development and their interactions, including hypothesis testing, and 3) exploratory analysis combining behavioral and brain data. The intervention includes intensive art classes once a week, and incremental home training over two years, with the CG receiving six annual cultural outings. This study examines the potential benefits of intensive group arts education, especially contrasting music with visual arts, on EF development in children. It will investigate how artistic enrichment potentially influences the presumed typical transition from a more unified to a more multifaceted EF structure around age eight, comparing these findings against a minimally enriched active control group. This research could significantly influence the incorporation of intensive art interventions in standard curricula. The project was accepted after peer-review by the Swiss National Science Foundation (SNSF no. 100014_214977) on March 29, 2023. The study protocol received approval from the Cantonal Commission for Ethics in Human Research of Geneva (CCER, BASEC-ID 2023-01016), which is part of Swiss ethics, on October 25, 2023. The study is registered at clinicaltrials.gov (NCT05912270).
对学龄初期大脑发育的最佳刺激仍不明确。目前的研究表明,与其他艺术形式相比,音乐训练对儿童的执行功能(EF)有更深远的影响。目前最缺乏的是认知神经科学领域的大规模、长期、真正的随机对照试验(RCT),将音乐器乐训练(MIP)与其他艺术形式和对照组(CG)进行比较。本研究旨在填补这一空白,利用机器学习开发一个多变量模型,跟踪学龄儿童在接受或不接受音乐或其他艺术培训期间大脑与 EF 发展的相互联系。该研究计划招募 150 名 6-8 岁的儿童,并将他们随机分配到三个小组:课堂管弦乐组(OC)、视觉艺术组(VA)和对照组(CG)。预计减员率为 30%,每组至少保留 35 名参与者。研究包括三个分析阶段:1)将 EF、大脑数据、年龄、性别和社会经济地位相关联的基线分析;2)各组之间和不同时期 EF 大脑和行为发展及其相互作用的比较,包括假设检验;3)结合行为和大脑数据的探索性分析。干预措施包括每周一次的强化艺术课程和两年的渐进式家庭训练,以及 CG 每年六次的文化郊游。本研究探讨了强化集体艺术教育(尤其是音乐与视觉艺术的对比)对儿童 EF 发展的潜在益处。本研究将探讨丰富的艺术教育如何潜在地影响儿童在八岁左右从较为统一的 EF 结构向较为多面的 EF 结构的典型过渡,并将这些研究结果与一个最低限度丰富的积极对照组进行比较。这项研究可能会对将强化艺术干预纳入标准课程产生重大影响。该项目经同行评审后于 2023 年 3 月 29 日获得瑞士国家科学基金会(SNSF 编号:100014_214977)的认可。研究方案于 2023 年 10 月 25 日获得日内瓦州人类研究伦理委员会(CCER,BASEC-ID 2023-01016)的批准,该委员会是瑞士伦理的一部分。该研究已在 clinicaltrials.gov 注册(NCT05912270)。
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引用次数: 0
Panax notoginseng saponins prevent dementia and oxidative stress in brains of SAMP8 mice by enhancing mitophagy 三七皂苷通过增强有丝分裂防止 SAMP8 小鼠大脑痴呆和氧化应激
2区 医学 Q1 Medicine Pub Date : 2024-04-04 DOI: 10.1186/s12906-024-04403-7
Yingying Yang, Wenya Chen, Zhenmei Lin, Yijing Wu, Yuqing Li, Xing Xia
Mitochondrial dysfunction is one of the distinctive features of neurons in patients with Alzheimer’s disease (AD). Intraneuronal autophagosomes selectively phagocytose and degrade the damaged mitochondria, mitigating neuronal damage in AD. Panax notoginseng saponins (PNS) can effectively reduce oxidative stress and mitochondrial damage in the brain of animals with AD, but their exact mechanism of action is unknown. Senescence-accelerated mouse prone 8 (SAMP8) mice with age-related AD were treated with PNS for 8 weeks. The effects of PNS on learning and memory abilities, cerebral oxidative stress status, and hippocampus ultrastructure of mice were observed. Moreover, changes of the PTEN-induced putative kinase 1 (PINK1)-Parkin, which regulates ubiquitin-dependent mitophagy, and the recruit of downstream autophagy receptors were investigated. PNS attenuated cognitive dysfunction in SAMP8 mice in the Morris water maze test. PNS also enhanced glutathione peroxidase and superoxide dismutase activities, and increased glutathione levels by 25.92% and 45.55% while inhibiting 8-hydroxydeoxyguanosine by 27.74% and the malondialdehyde production by 34.02% in the brains of SAMP8 mice. Our observation revealed the promotion of mitophagy, which was accompanied by an increase in microtubule-associated protein 1 light chain 3 (LC3) mRNA and 70.00% increase of LC3-II/I protein ratio in the brain tissues of PNS-treated mice. PNS treatment increased Parkin mRNA and protein expression by 62.80% and 43.80%, while increasing the mRNA transcription and protein expression of mitophagic receptors such as optineurin, and nuclear dot protein 52. PNS enhanced the PINK1/Parkin pathway and facilitated mitophagy in the hippocampus, thereby preventing cerebral oxidative stress in SAMP8 mice. This may be a mechanism contributing to the cognition-improvement effect of PNS.
线粒体功能障碍是阿尔茨海默病患者神经元的显著特征之一。神经元内的自噬体可选择性地吞噬和降解受损的线粒体,减轻阿尔茨海默病对神经元的损伤。三七皂苷(PNS)能有效减轻AD动物大脑中的氧化应激和线粒体损伤,但其确切的作用机制尚不清楚。研究人员用 PNS 对患有老年性注意力缺失症的衰老加速小鼠易感基因 8(SAMP8)小鼠进行了为期 8 周的治疗。观察了 PNS 对小鼠学习和记忆能力、大脑氧化应激状态和海马超微结构的影响。此外,还研究了调控泛素依赖性有丝分裂的PTEN诱导的推定激酶1(PINK1)-Parkin的变化以及下游自噬受体的招募情况。在莫里斯水迷宫测试中,PNS 可减轻 SAMP8 小鼠的认知功能障碍。PNS 还提高了谷胱甘肽过氧化物酶和超氧化物歧化酶的活性,谷胱甘肽水平分别提高了 25.92% 和 45.55%,同时抑制了 8-羟基脱氧鸟苷 27.74% 和丙二醛产生 34.02%。我们的观察结果表明,PNS 治疗小鼠脑组织中的微管相关蛋白 1 轻链 3(LC3)mRNA 增加,LC3-II/I 蛋白比值增加 70.00%,从而促进了有丝分裂。PNS 治疗可使 Parkin mRNA 和蛋白表达量分别增加 62.80% 和 43.80%,同时还可增加有丝分裂受体(如 optineurin)和核点蛋白 52 的 mRNA 转录量和蛋白表达量。PNS 增强了 PINK1/Parkin 通路,促进了海马中的有丝分裂,从而防止了 SAMP8 小鼠的脑氧化应激。这可能是 PNS 改善认知效果的一个机制。
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BMC Complementary and Alternative Medicine
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