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Regulation of hippocampal NMDA receptors by magnesium and glycine during development. 镁和甘氨酸在发育过程中对海马NMDA受体的调节。
N W Kleckner, R Dingledine

N-Methyl-D-aspartate (NMDA) receptors play an important role in the development of neuronal connections in the retina and visual cortex, and in synaptic plasticity in the hippocampus. The objective of this study was to determine whether the sensitivity of hippocampal NMDA receptors to magnesium, glycine or NMDA changes during development. Xenopus oocytes were injected with mRNA prepared from hippocampi from rats of different ages, and NMDA receptor properties studied under voltage clamp. Voltage-dependent block of the NMDA receptor by magnesium was studied with voltage steps of -90 mV to -30 mV, in increments of 10 mV, during application of 100 microM NMDA, 3 microM glycine and 0-1000 microM Mg2+. The IC50 of Mg2+ for blocking NMDA receptor-mediated currents varied e-fold (2.72-fold) for approximately every 15 mV of membrane potential in the middle range of membrane potential (-70 to -50 mV), but the relationship between log[IC50] for Mg2+ and membrane potential was not linear, as would be expected for simple channel block. The slopes of the curves did not change with development, indicating no change in the voltage-dependence of Mg2+ block with age. However, the IC50 of Mg2+ block did change with age at every membrane potential tested. NMDA receptors expressed from mRNA isolated from 14-15 day old rats were nearly 2-fold less sensitive to block by Mg2+ (IC50 = 33 microM at -60 mV) than those from 1-2 day old rats (IC50 = 18 microM).(ABSTRACT TRUNCATED AT 250 WORDS)

n -甲基- d -天冬氨酸(NMDA)受体在视网膜和视觉皮层神经元连接的发育以及海马突触可塑性中发挥重要作用。本研究的目的是确定海马NMDA受体对镁、甘氨酸或NMDA的敏感性是否在发育过程中发生变化。将不同年龄大鼠海马制备的mRNA注入爪蟾卵母细胞,在电压箝位下研究NMDA受体的特性。在NMDA浓度为100 μ m、甘氨酸浓度为3 μ m、Mg2+浓度为0 ~ 1000 μ m的条件下,以-90 mV ~ -30 mV为电压阶,以10 mV为增量,研究了镁对NMDA受体的电压依赖性阻断作用。在膜电位的中间范围内(-70 ~ -50 mV), Mg2+阻断NMDA受体介导的电流的IC50大约每15 mV变化e倍(2.72倍),但Mg2+与膜电位之间的log[IC50]不是线性关系,正如简单通道阻断所预期的那样。曲线的斜率不随发育变化,说明Mg2+块体的电压依赖性不随年龄变化。而Mg2+阻滞的IC50在各膜电位下均随年龄变化。从14-15日龄大鼠分离的mRNA中表达的NMDA受体对Mg2+阻滞的敏感性(IC50 = 33 μ m, -60 mV)比1-2日龄大鼠(IC50 = 18 μ m)低近2倍。(摘要删节250字)
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Brain research. Molecular brain research
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