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Metagenomic symphony of the intestinal ecosystem: How the composition affects the mind 肠道生态系统的元基因组交响乐:构成如何影响心灵
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-10-04 DOI: 10.1016/j.bbi.2024.09.033
Stefanie Malan-Müller , David Martín-Hernández , Javier R. Caso , Jelle Matthijnssens , Amanda Rodríguez-Urrutia , Christopher A. Lowry , Juan C. Leza
Mental health disorders and neurodegenerative diseases place a heavy burden on patients and societies, and, although great strides have been made to understand the pathophysiology of these conditions, advancement in drug development is lagging. The importance of gastrointestinal health in maintaining overall health and preventing disease is not a new concept. Hundreds of years ago, healers from various cultures and civilizations recognized the crucial role of the gut in sustaining health. More than a century ago, scientists began exploring the restorative effects of probiotics, marking the early recognition of the importance of gut microbes. The omics era brought more enlightenment and enabled researchers to identify the complexity of the microbial ecosystems we harbour, encompassing bacteria, eukaryotes (including fungi), archaea, viruses, and other microorganisms. The extensive genetic capacity of the microbiota is dynamic and influenced by the environment. The microbiota therefore serves as a significant entity within us, with evolutionarily preserved functions in host metabolism, immunity, development, and behavior. The significant role of the bacterial gut microbiome in mental health and neurodegenerative disorders has been realized and described within the framework of the microbiota-gut-brain axis. However, the bacterial members do not function unaccompanied, but rather in concert, and there is a substantial knowledge gap regarding the involvement of non-bacterial microbiome members in these disorders. In this review, we will explore the current literature that implicates a role for the entire metagenomic ensemble, and how their complex interkingdom relationships could influence CNS functioning in mental health disorders and neurodegenerative diseases.
精神疾病和神经退行性疾病给患者和社会带来了沉重的负担,尽管人们在了解这些疾病的病理生理学方面取得了长足的进步,但药物开发方面的进展却十分滞后。胃肠道健康对维持整体健康和预防疾病的重要性并不是一个新概念。数百年前,来自不同文化和文明的医学家就认识到肠道在维持健康方面的关键作用。一个多世纪前,科学家们开始探索益生菌的修复作用,标志着人们很早就认识到肠道微生物的重要性。omics时代带来了更多启迪,使研究人员能够识别我们体内微生物生态系统的复杂性,包括细菌、真核生物(包括真菌)、古生菌、病毒和其他微生物。微生物群广泛的遗传能力是动态的,并受到环境的影响。因此,微生物群是我们体内的一个重要实体,在宿主的新陈代谢、免疫、发育和行为方面具有进化过程中保留下来的功能。细菌肠道微生物群在心理健康和神经退行性疾病中的重要作用已在微生物群-肠道-大脑轴的框架内得到认识和描述。然而,细菌成员并不是孤立地发挥作用,而是相互配合,而关于非细菌微生物群成员在这些疾病中的参与,还存在很大的知识空白。在这篇综述中,我们将探讨目前有哪些文献表明整个元基因组的作用,以及它们之间复杂的关系如何影响精神疾病和神经退行性疾病的中枢神经系统功能。
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引用次数: 0
Pathways to maternal health inequities: Structural racism, sleep, and physiological stress 孕产妇健康不平等的途径:结构性种族主义、睡眠和生理压力。
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-10-02 DOI: 10.1016/j.bbi.2024.09.037
Lisa M. Christian , Ryan L. Brown , Judith E. Carroll , Julian F. Thayer , Tené T. Lewis , Shannon L. Gillespie , Christopher P. Fagundes
Racial inequities in health are vast and well-documented, particularly regarding maternal and infant health. Sleep health, including but not limited to duration and quality, is central to overall health and well-being. However, research has not adequately addressed how racism embedded in structures and systems, in addition to individual experiences, may affect maternal health by impacting sleep. In this critical review, we aim to 1) synthesize findings, emphasizing collaborative studies within our group, 2) highlight gaps in knowledge, and 3) propose a theoretical framework and methodological approach for moving the field forward. Specifically, we focus on findings and future directions linking perinatal sleep, cardiovascular and immune function, and racial disparities in maternal health. Because too few studies look beyond individual-level determinants of sleep deficiencies among Black Americans, we assert a critical need for research that bridges multiple levels of analysis (e.g., individual, community, society) and provides recommendations for specific health parameters that researchers in this area can target. Although the need to understand and address perinatal health disparities is clear, the goal of identifying multilevel mechanisms underlying how racism in one’s environment and daily life may interact to affect health extends far beyond pregnancy research.
种族在健康方面的不平等是巨大的,也是有据可查的,尤其是在母婴健康方面。睡眠健康,包括但不限于睡眠时间和质量,对整体健康和幸福至关重要。然而,除了个人经历之外,研究还没有充分探讨结构和系统中的种族主义如何通过影响睡眠来影响孕产妇的健康。在这篇重要综述中,我们旨在:1)综合研究结果,强调我们小组内的合作研究;2)突出知识差距;3)提出理论框架和方法论,以推动该领域的发展。具体来说,我们将重点放在将围产期睡眠、心血管和免疫功能以及孕产妇健康中的种族差异联系起来的研究结果和未来方向上。由于很少有研究关注美国黑人睡眠不足的个人层面以外的决定因素,我们认为亟需开展连接多个分析层面(如个人、社区、社会)的研究,并为该领域研究人员可针对的具体健康参数提供建议。虽然了解和解决围产期健康差异的必要性是显而易见的,但确定环境和日常生活中的种族主义如何相互作用影响健康的多层次机制这一目标远远超出了妊娠研究的范围。
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引用次数: 0
Communication of inflammation 炎症的传播。
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-30 DOI: 10.1016/j.bbi.2024.09.036
Michael B. Hennessy
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引用次数: 0
Microglial TREM2 promotes phagocytic clearance of damaged neurons after status epilepticus 小胶质细胞 TREM2 促进对癫痫状态后受损神经元的吞噬清除。
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-29 DOI: 10.1016/j.bbi.2024.09.034
Dale B. Bosco , Vaclav Kremen , Koichiro Haruwaka , Shunyi Zhao , Lingxiao Wang , Blake A. Ebner , Jiaying Zheng , Manling Xie , Aastha Dheer , Jadyn F. Perry , Abhijeet Barath , Aivi T. Nguyen , Gregory A. Worrell , Long-Jun Wu
In the central nervous system, triggering receptor expressed on myeloid cells 2 (TREM2) is exclusively expressed by microglia and is critical for microglial proliferation, migration, and phagocytosis. Microglial TREM2 plays an important role in neurodegenerative diseases, such as Alzheimer’s disease and amyotrophic lateral sclerosis. However, little is known about how TREM2 affects microglial function within epileptogenesis. To investigate this, we utilized male TREM2 knockout (KO) mice within the intra-amygdala kainic acid seizure model. Electroencephalographic analysis, immunocytochemistry, and RNA sequencing revealed that TREM2 deficiency significantly promoted seizure-induced pathology. We found that TREM2 KO increased both the severity of acute status epilepticus and the number of spontaneous recurrent seizures characteristic of chronic focal epilepsy. Phagocytic clearance of damaged neurons by microglia was also impaired by TREM2 KO and reduced phagocytic activity correlated with increased spontaneous seizures. Analysis of human tissue from patients who underwent surgical resection for drug resistant temporal lobe epilepsy also showed a negative correlation between expression of the microglial phagocytic marker CD68 and focal to bilateral tonic-clonic generalized seizure history. These results indicate that microglial TREM2 and phagocytic activity are important to epileptogenic pathology.
在中枢神经系统中,髓系细胞上表达的触发受体 2(TREM2)专门由小胶质细胞表达,对小胶质细胞的增殖、迁移和吞噬作用至关重要。小胶质细胞 TREM2 在阿尔茨海默病和肌萎缩侧索硬化症等神经退行性疾病中发挥着重要作用。然而,人们对 TREM2 如何影响癫痫发生过程中的小胶质细胞功能知之甚少。为了研究这个问题,我们利用雄性 TREM2 基因敲除(KO)小鼠在小鼠杏仁核内凯尼酸癫痫模型中进行研究。脑电图分析、免疫细胞化学和 RNA 测序显示,TREM2 的缺失会显著促进癫痫发作诱发的病理变化。我们发现,TREM2 KO会增加急性癫痫状态的严重程度和慢性局灶性癫痫特征性的自发性复发性发作次数。小胶质细胞对受损神经元的吞噬清除能力也会因 TREM2 KO 而受损,吞噬活性的降低与自发性癫痫发作的增加有关。对因耐药性颞叶癫痫而接受手术切除的患者的人体组织进行的分析也显示,小胶质细胞吞噬标记物 CD68 的表达与局灶性至双侧强直阵挛性全身性癫痫发作史呈负相关。这些结果表明,小胶质细胞 TREM2 和吞噬活性对致痫病理学非常重要。
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引用次数: 0
Upregulation of delta opioid receptor by meningeal interleukin-10 prevents relapsing pain 脑膜白细胞介素-10上调δ类阿片受体可预防复发性疼痛
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-29 DOI: 10.1016/j.bbi.2024.09.031
Kufreobong E. Inyang , Jaewon Sim , Kimberly B. Clark , Matan Geron , Karli Monahan , Christine Evans , Patrick O’Connell , Sophie Laumet , Bo Peng , Jiacheng Ma , Cobi J. Heijnen , Robert Dantzer , Grégory Scherrer , Annemieke Kavelaars , Matthew Bernard , Yasser A. Aldhamen , Joseph K. Folger , Alexis Bavencoffe , Geoffroy Laumet
Chronic pain often includes periods of transient amelioration and even remission that alternate with severe relapsing pain. While most research on chronic pain has focused on pain development and maintenance, there is a critical unmet need to better understand the mechanisms that underlie pain remission and relapse. We found that interleukin (IL)-10, a pain resolving cytokine, is produced by resident macrophages in the spinal meninges during remission from pain and signaled to IL-10 receptor-expressing sensory neurons. Using unbiased RNA-sequencing, we identified that IL-10 upregulated expression and antinociceptive activity of δ-opioid receptor (δOR) in the dorsal root ganglion. Genetic or pharmacological inhibition of either IL-10 signaling or δOR triggered relapsing pain. Overall, our findings, from electrophysiology, genetic manipulation, flow cytometry, pharmacology, and behavioral approaches, indicate that remission of pain is not simply a return to the naïve state. Instead, remission is an adapted homeostatic state associated with lasting pain vulnerability resulting from persisting neuroimmune interactions within the nociceptive system. Broadly, this sheds light on the elusive mechanisms underlying recurrence a common aspect across various chronic pain conditions.
慢性疼痛通常会有短暂的缓解期,甚至缓解期与严重的复发期交替出现。虽然大多数有关慢性疼痛的研究都集中在疼痛的发展和维持上,但更好地了解疼痛缓解和复发的机制仍是一项尚未满足的重大需求。我们发现,白细胞介素(IL)-10 是一种缓解疼痛的细胞因子,在疼痛缓解期间由脊髓脑膜中的常驻巨噬细胞产生,并向表达 IL-10 受体的感觉神经元发出信号。通过无偏见的 RNA 序列分析,我们发现 IL-10 能上调背根神经节中δ-阿片受体(δOR)的表达和抗痛觉活性。对IL-10信号或δOR的遗传或药物抑制都会引发复发性疼痛。总之,我们通过电生理学、遗传操作、流式细胞术、药理学和行为学方法得出的研究结果表明,疼痛的缓解并不是简单地恢复到天真状态。相反,缓解是一种适应性平衡状态,与痛觉系统内持续的神经免疫相互作用导致的持久疼痛脆弱性有关。从广义上讲,这揭示了各种慢性疼痛的共同点--复发--的难以捉摸的内在机制。
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引用次数: 0
Inflammatory pain resolution by mouse serum-derived small extracellular vesicles 小鼠血清源性小细胞外囊泡缓解炎性疼痛。
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-29 DOI: 10.1016/j.bbi.2024.09.032
Zhucheng Lin , Xuan Luo , Jason R. Wickman , Deepa Reddy , Jason T. DaCunza , Richa Pande , Yuzhen Tian , Ezgi E. Kasimoglu , Vivian Triana , Jingyun Lee , Cristina M. Furdui , Desmond Pink , Ahmet Sacan , Seena K. Ajit
Current treatments for chronic pain have limited efficacy and significant side effects, warranting research on alternative strategies for pain management. One approach involves using small extracellular vesicles (sEVs), or exosomes, to transport beneficial biomolecular cargo to aid pain resolution. Exosomes are 30–150 nm sEVs that can be beneficial or harmful depending on their source and cargo composition. We report a comprehensive multi-modal analysis of different aspects of sEV characterization, miRNAs, and protein markers across sEV sources. To investigate the short- and long-term effects of mouse serum-derived sEVs in pain modulation, sEVs from naïve control or spared nerve injury (SNI) model male donor mice were injected intrathecally into naïve male recipient mice. These sEVs transiently increased basal mechanical thresholds, an effect mediated by opioid signaling as this outcome was blocked by naltrexone. Mass spectrometry of sEVs detected endogenous opioid peptide leu-enkephalin. sEVs from naïve female mice have higher levels of leu-enkephalin compared to male, matching the analgesic onset of leu-enkephalin in male recipient mice. In investigating the long-term effect of sEVs, we observed that a single prophylactic intrathecal injection of sEVs two weeks prior to induction of the pain model in recipient mice accelerated recovery from inflammatory pain after complete Freund’s adjuvant (CFA) injection. Our exploratory studies examining immune cell populations in spinal cord and dorsal root ganglion using ChipCytometry suggested alterations in immune cell populations 14 days post-CFA. Flow cytometry confirmed increases in CD206+ macrophages in the spinal cord in sEV-treated mice. Collectively, these studies demonstrate multiple mechanisms by which sEVs can attenuate pain.
目前治疗慢性疼痛的方法疗效有限,而且副作用很大,因此有必要研究疼痛管理的替代策略。其中一种方法是利用小细胞外囊泡(sEVs)或外泌体来运输有益的生物分子货物,以帮助缓解疼痛。外泌体是一种 30-150 纳米的 sEV,根据其来源和货物成分的不同,可能有益也可能有害。我们报告了对各种 sEV 来源的 sEV 特征、miRNA 和蛋白质标记物的不同方面进行的全面多模式分析。为了研究小鼠血清来源的 sEV 对疼痛调节的短期和长期影响,我们将来自天真对照组或幸免神经损伤(SNI)模型雄性供体小鼠的 sEV 经鞘内注射到天真雄性受体小鼠体内。这些 sEVs 可短暂提高基础机械阈值,这种效应由阿片类药物信号介导,因为纳曲酮可阻断这种效应。与雄性小鼠相比,来自天真雌性小鼠的 sEVs 含有更高水平的 leu-enkephalin,并且在雄性受体中显示出更强的效应。在研究 sEVs 的长期效应时,我们观察到,在受体小鼠疼痛模型诱导前两周进行一次预防性鞘内注射 sEVs,可加快完全弗氏佐剂(CFA)注射后炎症性疼痛的恢复。我们利用芯片流式细胞术对脊髓和背根神经节中的免疫细胞群进行了探索性研究,结果表明,在注射完全弗氏佐剂(CFA)14 天后,免疫细胞群发生了变化。流式细胞术证实了经 sEV 处理的小鼠脊髓中 CD206+ 巨噬细胞的增加。总之,这些研究证明了 sEV 可减轻疼痛的多种机制。
{"title":"Inflammatory pain resolution by mouse serum-derived small extracellular vesicles","authors":"Zhucheng Lin ,&nbsp;Xuan Luo ,&nbsp;Jason R. Wickman ,&nbsp;Deepa Reddy ,&nbsp;Jason T. DaCunza ,&nbsp;Richa Pande ,&nbsp;Yuzhen Tian ,&nbsp;Ezgi E. Kasimoglu ,&nbsp;Vivian Triana ,&nbsp;Jingyun Lee ,&nbsp;Cristina M. Furdui ,&nbsp;Desmond Pink ,&nbsp;Ahmet Sacan ,&nbsp;Seena K. Ajit","doi":"10.1016/j.bbi.2024.09.032","DOIUrl":"10.1016/j.bbi.2024.09.032","url":null,"abstract":"<div><div>Current treatments for chronic pain have limited efficacy and significant side effects, warranting research on alternative strategies for pain management. One approach involves using small extracellular vesicles (sEVs), or exosomes, to transport beneficial biomolecular cargo to aid pain resolution. Exosomes are 30–150 nm sEVs that can be beneficial or harmful depending on their source and cargo composition. We report a comprehensive multi-modal analysis of different aspects of sEV characterization, miRNAs, and protein markers across sEV sources. To investigate the short- and long-term effects of mouse serum-derived sEVs in pain modulation, sEVs from naïve control or spared nerve injury (SNI) model male donor mice were injected intrathecally into naïve male recipient mice. These sEVs transiently increased basal mechanical thresholds, an effect mediated by opioid signaling as this outcome was blocked by naltrexone. Mass spectrometry of sEVs detected endogenous opioid peptide leu-enkephalin. sEVs from naïve female mice have higher levels of leu-enkephalin compared to male, matching the analgesic onset of leu-enkephalin in male recipient mice. In investigating the long-term effect of sEVs, we observed that a single prophylactic intrathecal injection of sEVs two weeks prior to induction of the pain model in recipient mice accelerated recovery from inflammatory pain after complete Freund’s adjuvant (CFA) injection. Our exploratory studies examining immune cell populations in spinal cord and dorsal root ganglion using ChipCytometry suggested alterations in immune cell populations 14 days post-CFA. Flow cytometry confirmed increases in CD206<sup>+</sup> macrophages in the spinal cord in sEV-treated mice. Collectively, these studies demonstrate multiple mechanisms by which sEVs can attenuate pain.</div></div>","PeriodicalId":9199,"journal":{"name":"Brain, Behavior, and Immunity","volume":"123 ","pages":"Pages 422-441"},"PeriodicalIF":8.8,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sleep loss-induced oncogenic pathways are mediated via the neuron-specific interleukin-1 receptor accessory protein (AcPb) 睡眠不足诱导的致癌途径是通过神经元特异性白细胞介素-1受体附属蛋白(AcPb)介导的。
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-28 DOI: 10.1016/j.bbi.2024.09.029
Yool Lee , Erika L. English , Catherine M. Schwartzmann , Yiyong Liu , James M. Krueger
Interleukin-1β (IL1), a pleiotropic cytokine, is involved in sleep regulation, tumor ontogeny, and immune responses. IL1 receptor adaptor proteins, including the IL1 receptor accessory protein (AcP), and its neuron-specific isoform, AcPb, are required for IL1 signaling. The AcPb isoform is resultant from alternate splicing of the AcP transcript. Our previous studies using AcPb null (AcPb-/-) mice characterized its participation in sleep regulation and emergent neuronal/glial network properties. Here, we investigated the impact of acute sleep disruption (SD) on brain cancer-related pathways in wild-type (WT) and AcPb-/- mice, employing RNA sequencing methods. In WT mice, SD increased AcPb mRNA levels, but not AcP mRNA, confirming prior similar work in rats. Transcriptome and pathway enrichment analyses demonstrated significant alterations in cancer, immune, and viral disease-related pathways in WT mice after SD, which were attenuated in AcPb-/- mice including multiple upregulated Src phosphorylation-signaling-dependent genes associated with cancer progression and metastasis. Our RNAseq findings, were analyzed within the context of The Cancer Genome Atlas Program (TCGA) data base; revealing an upregulation of sleep- and cancer-linked genes (e.g., IL-17B, IL-17RA, LCN2) across various tumors, including brain tumors, compared to normal tissues. Sleep-linked factors, identified through TCGA analyses, significantly impact patient prognosis and survival, particularly in low-grade glioma (LGG) and glioblastoma multiforme (GBM) patients. Overall, our findings suggest that SD promotes a pro-tumor environment through AcPb-modulated pathways.
白细胞介素-1β(IL1)是一种多效细胞因子,参与睡眠调节、肿瘤的发生和免疫反应。IL1 受体适配蛋白,包括 IL1 受体附属蛋白(AcP)及其神经元特异异构体 AcPb,是 IL1 信号传导所必需的。AcPb 异构体是 AcP 转录本交替剪接的结果。我们之前使用 AcPb 空值(AcPb-/-)小鼠进行的研究揭示了它参与睡眠调节和神经元/胶质细胞网络特性的特点。在这里,我们采用 RNA 测序方法研究了急性睡眠中断(SD)对野生型(WT)和 AcPb-/- 小鼠脑癌相关通路的影响。在WT小鼠中,SD增加了AcPb mRNA水平,但没有增加AcP mRNA水平,这证实了之前在大鼠中进行的类似研究。转录组和通路富集分析表明,SD 后 WT 小鼠的癌症、免疫和病毒性疾病相关通路发生了显著变化,而 AcPb-/- 小鼠的这些变化有所减弱,其中包括与癌症进展和转移相关的多个 Src 磷酸化信号依赖基因的上调。我们在癌症基因组图谱计划(TCGA)数据库中对 RNAseq 研究结果进行了分析,结果显示,与正常组织相比,包括脑肿瘤在内的各种肿瘤中与睡眠和癌症相关的基因(如 IL-17B、IL-17RA、LCN2)都出现了上调。通过TCGA分析确定的睡眠相关因素对患者的预后和生存有重大影响,尤其是在低级别胶质瘤(LGG)和多形性胶质母细胞瘤(GBM)患者中。总之,我们的研究结果表明,SD通过AcPb调控通路促进了有利于肿瘤的环境。
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引用次数: 0
Pathophysiology, blood biomarkers, and functional deficits after intimate partner violence-related brain injury: Insights from emergency department patients and a new rat model 亲密伴侣暴力相关脑损伤后的病理生理学、血液生物标志物和功能障碍:来自急诊科患者和新大鼠模型的启示。
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-28 DOI: 10.1016/j.bbi.2024.09.030
Mujun Sun , Georgia F. Symons , Gershon Spitz , William T. O’Brien , Tamara L Baker , Jianjia Fan , Beatriz D. Martins , Josh Allen , Lauren P. Giesler , Richelle Mychasiuk , Paul van Donkelaar , Justin Brand , Brian Christie , Terence J. O’Brien , Michael J. O’Sullivan , Biswadev Mitra , Cheryl Wellington , Stuart J. McDonald , Sandy R. Shultz
Intimate partner violence is a serious, but underappreciated, issue that predominantly affects women and often results in concussion (i.e., mild traumatic brain injury). However, concussion in intimate partner violence is unique because it often involves a concomitant strangulation which may exacerbate or alter the physiology and clinical presentation of the brain injury. Therefore, here we conducted human and rodent studies to provide insight into knowledge gaps related to the detection, pathophysiology, and functional consequences of intimate partner violence-related brain injury. We conducted the first study to analyze blood biomarkers and symptoms of brain injury in intimate partner violence patients presenting to an emergency department within 72 h of concussion. Intimate partner violence concussion patients, some of whom had also experienced a concomitant strangulation, had elevated serum neurofilament light and worse brain injury symptoms compared to healthy control, orthopedic trauma, and non-intimate partner violence concussion groups. We also developed the first rat model of non-fatal strangulation and examined the consequences of strangulation and concussion in isolation and in combination on pathophysiology, blood biomarkers, and behavior at 2 h and 1wk post-injury. Rats exposed to combined strangulation and concussion had exacerbated motor and cognitive deficits, neuroinflammation, and serum glial fibrillary acidic protein levels compared with either injury in isolation. Taken together, these rodent findings demonstrate that a concomitant strangulation modifies and exacerbates concussion pathophysiology, biomarkers, and functional consequences. Overall, these findings provide novel insights into intimate partner violence-related brain injury and provides a foundation for future translational studies.
亲密伴侣暴力是一个严重但未得到充分重视的问题,它主要影响女性,并经常导致脑震荡(即轻微脑外伤)。然而,亲密伴侣暴力中的脑震荡是独一无二的,因为它往往同时涉及到勒死,这可能会加剧或改变脑损伤的生理和临床表现。因此,我们在此开展了人类和啮齿动物研究,以深入了解与亲密伴侣暴力相关的脑损伤的检测、病理生理学和功能性后果方面的知识差距。我们进行了第一项研究,分析了在脑震荡后 72 小时内到急诊科就诊的亲密伴侣暴力患者的血液生物标志物和脑损伤症状。与健康对照组、骨科创伤组和非亲密伴侣暴力脑震荡组相比,亲密伴侣暴力脑震荡患者的血清神经丝蛋白轻度升高,脑损伤症状加重,其中一些患者还同时经历了勒死。我们还建立了首个非致命性扼颈大鼠模型,并研究了扼颈和脑震荡单独或合并对损伤后 2 小时和 1 周内的病理生理学、血液生物标志物和行为的影响。与单独受到其中一种伤害的大鼠相比,同时受到扼杀和脑震荡伤害的大鼠运动和认知障碍、神经炎症和血清胶质纤维酸性蛋白水平都有所加剧。总之,这些啮齿类动物的研究结果表明,同时发生的绞杀会改变和加剧脑震荡的病理生理学、生物标志物和功能性后果。总之,这些发现为亲密伴侣暴力相关脑损伤提供了新的见解,并为未来的转化研究奠定了基础。
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引用次数: 0
Exploring the use of immunomethylomics in the characterization of depressed patients: A proof-of-concept study 探索免疫组学在抑郁症患者特征描述中的应用:概念验证研究
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-26 DOI: 10.1016/j.bbi.2024.09.026
Evelien Van Assche , Christa Hohoff , Ecem Su Atil , Sophia M. Wissing , Alessandro Serretti , Chiara Fabbri , Claudia Pisanu , Alessio Squassina , Alessandra Minelli , European College of Neuropsychopharmacology (ECNP) Pharmacogenomics & Transcriptomics Network , Bernhard T. Baune
Alterations in DNA methylation and inflammation could represent valid biomarkers for the stratification of patients with major depressive disorder (MDD). This study explored the use of DNA-methylation based immunological cell-type profiles in the context of MDD and symptom severity over time.
In 119 individuals with MDD, DNA-methylation was assessed on whole blood using the Illumina Infinium MethylationEPIC 850 k BeadChip. Quality control and data processing, as well as cell type estimation was conducted using the RnBeads package. The cell type composition was estimated using epigenome-wide DNA methylation signatures, applying the Houseman method, considering six cell types (neutrophils, natural killer cells (NK), B cells, CD4+ T cells, CD8+ T cells and monocytes). Two cytokines (IL-6 and IL-1β) and hsCRP were quantified in serum. We performed a hierarchical cluster analysis on the six estimated cell-types and tested the differences between these clusters in relation to the two cytokines and hsCRP, depression severity at baseline, and after 6 weeks of treatment (celecoxib/placebo + vortioxetine). We performed a second cluster analysis with cell-types and cytokines combined. ANCOVA was used to test for differences across clusters. We applied the Bonferroni correction.
After quality control, we included 113 participants. Two clusters were identified, cluster 1 was high in CD4+ cells and NK, cluster 2 was high in CD8+ T-cells and B-cells, with similar fractions of neutrophils and monocytes. The clusters were not associated with either of the two cytokines and hsCRP, or depression severity at baseline, but cluster 1 showed higher depression severity after 6 weeks, corrected for baseline (p = 0.0060). The second cluster analysis found similar results: cluster 1 was low in CD8+ T-cells, B-cells, and IL-1β. Cluster 2 was low in CD4+ cells and natural killer cells. Neutrophils, monocytes, IL-6 and hsCRP were not different between the clusters. Participants in cluster 1 showed higher depression severity at baseline than cluster 2 (p = 0.034), but no difference in depression severity after 6 weeks.
DNA-methylation based cell-type profiles may be valuable in the immunological characterization and stratification of patients with MDD. Future models should consider the inclusion of more cell-types and cytokines for better a prediction of treatment outcomes.
DNA 甲基化和炎症的改变可能是对重度抑郁症(MDD)患者进行分层的有效生物标志物。本研究探讨了在MDD和症状严重程度随时间变化的背景下,使用基于DNA甲基化的免疫细胞类型图谱。使用 Illumina Infinium MethylationEPIC 850 k BeadChip 对 119 名 MDD 患者的全血进行了 DNA 甲基化评估。使用 RnBeads 软件包进行了质量控制、数据处理和细胞类型估计。细胞类型组成是通过表观基因组范围的 DNA 甲基化特征来估算的,应用了 Houseman 方法,考虑了六种细胞类型(中性粒细胞、自然杀伤细胞(NK)、B 细胞、CD4 + T 细胞、CD8 + T 细胞和单核细胞)。对血清中的两种细胞因子(IL-6 和 IL-1β)和 hsCRP 进行了定量。我们对估计出的六种细胞类型进行了分层聚类分析,并检测了这些聚类与两种细胞因子和 hsCRP、基线抑郁严重程度以及 6 周治疗(塞来昔布/安慰剂+伏替西汀)后抑郁严重程度之间的差异。我们结合细胞类型和细胞因子进行了第二次聚类分析。方差分析用于检验不同聚类之间的差异。我们采用了 Bonferroni 校正。经过质量控制后,我们纳入了 113 名参与者。我们发现了两个集群,集群 1 的 CD4 + 细胞和 NK 含量高,集群 2 的 CD8 + T 细胞和 B 细胞含量高,中性粒细胞和单核细胞的比例相似。聚类与两种细胞因子和 hsCRP 或基线抑郁严重程度无关,但聚类 1 在 6 周后显示出较高的抑郁严重程度,并对基线进行了校正(p = 0.0060)。第二次聚类分析发现了类似的结果:聚类 1 的 CD8 + T 细胞、B 细胞和 IL-1β 含量较低。第 2 组的 CD4 + 细胞和自然杀伤细胞含量较低。中性粒细胞、单核细胞、IL-6 和 hsCRP 在群组之间没有差异。群组 1 的参与者基线抑郁严重程度高于群组 2(p = 0.034),但 6 周后抑郁严重程度无差异。基于DNA甲基化的细胞类型图谱可能对MDD患者的免疫学特征描述和分层很有价值。未来的模型应考虑纳入更多的细胞类型和细胞因子,以更好地预测治疗结果。
{"title":"Exploring the use of immunomethylomics in the characterization of depressed patients: A proof-of-concept study","authors":"Evelien Van Assche ,&nbsp;Christa Hohoff ,&nbsp;Ecem Su Atil ,&nbsp;Sophia M. Wissing ,&nbsp;Alessandro Serretti ,&nbsp;Chiara Fabbri ,&nbsp;Claudia Pisanu ,&nbsp;Alessio Squassina ,&nbsp;Alessandra Minelli ,&nbsp;European College of Neuropsychopharmacology (ECNP) Pharmacogenomics & Transcriptomics Network ,&nbsp;Bernhard T. Baune","doi":"10.1016/j.bbi.2024.09.026","DOIUrl":"10.1016/j.bbi.2024.09.026","url":null,"abstract":"<div><div>Alterations in DNA methylation and inflammation could represent valid biomarkers for the stratification of patients with major depressive disorder (MDD). This study explored the use of DNA-methylation based immunological cell-type profiles in the context of MDD and symptom severity over time.</div><div>In 119 individuals with MDD, DNA-methylation was assessed on whole blood using the Illumina Infinium MethylationEPIC 850 k BeadChip. Quality control and data processing, as well as cell type estimation was conducted using the RnBeads package. The cell type composition was estimated using epigenome-wide DNA methylation signatures, applying the Houseman method, considering six cell types (neutrophils, natural killer cells (NK), B cells, CD4+ T cells, CD8+ T cells and monocytes). Two cytokines (IL-6 and IL-1β) and hsCRP were quantified in serum. We performed a hierarchical cluster analysis on the six estimated cell-types and tested the differences between these clusters in relation to the two cytokines and hsCRP, depression severity at baseline, and after 6 weeks of treatment (celecoxib/placebo + vortioxetine). We performed a second cluster analysis with cell-types and cytokines combined. ANCOVA was used to test for differences across clusters. We applied the Bonferroni correction.</div><div>After quality control, we included 113 participants. Two clusters were identified, cluster 1 was high in CD4+ cells and NK, cluster 2 was high in CD8+ T-cells and B-cells, with similar fractions of neutrophils and monocytes. The clusters were not associated with either of the two cytokines and hsCRP, or depression severity at baseline, but cluster 1 showed higher depression severity after 6 weeks, corrected for baseline (<em>p</em> = 0.0060). The second cluster analysis found similar results: cluster 1 was low in CD8+ T-cells, B-cells, and IL-1β. Cluster 2 was low in CD4+ cells and natural killer cells. Neutrophils, monocytes, IL-6 and hsCRP were not different between the clusters. Participants in cluster 1 showed higher depression severity at baseline than cluster 2 (<em>p</em> = 0.034), but no difference in depression severity after 6 weeks.</div><div>DNA-methylation based cell-type profiles may be valuable in the immunological characterization and stratification of patients with MDD. Future models should consider the inclusion of more cell-types and cytokines for better a prediction of treatment outcomes.</div></div>","PeriodicalId":9199,"journal":{"name":"Brain, Behavior, and Immunity","volume":"123 ","pages":"Pages 597-605"},"PeriodicalIF":8.8,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blockage of ATGL-mediated breakdown of lipid droplets in microglia alleviates neuroinflammatory and behavioural responses to lipopolysaccharides 阻断 ATGL 介导的小胶质细胞脂滴分解可减轻对脂多糖的神经炎症反应和行为反应
IF 8.8 2区 医学 Q1 IMMUNOLOGY Pub Date : 2024-09-24 DOI: 10.1016/j.bbi.2024.09.027
Josephine Louise Robb , Frédérick Boisjoly , Arturo Israel Machuca-Parra , Adeline Coursan , Romane Manceau , Danie Majeur , Demetra Rodaros , Khalil Bouyakdan , Karine Greffard , Jean-François Bilodeau , Anik Forest , Caroline Daneault , Matthieu Ruiz , Cyril Laurent , Nathalie Arbour , Sophie Layé , Xavier Fioramonti , Charlotte Madore , Stephanie Fulton , Thierry Alquier
Lipid droplets (LD) are triglyceride storing organelles that have emerged as an important component of cellular inflammatory responses. LD lipolysis via adipose triglyceride lipase (ATGL), the enzyme that catalyses the rate-limiting step of triglyceride lipolysis, regulates inflammation in peripheral immune and non-immune cells. ATGL elicits both pro- and anti-inflammatory responses in the periphery in a cell-type dependent manner. The present study determined the impact of ATGL inhibition and microglia-specific ATGL genetic loss-of-function on acute inflammatory and behavioural responses to pro-inflammatory insult. First, we evaluated the impact of lipolysis inhibition on lipopolysaccharide (LPS)-induced expression and secretion of cytokines and phagocytosis in mouse primary microglia cultures. Lipase inhibitors (ORlistat and ATGListatin) and LPS led to LD accumulation in microglia. Pan-lipase inhibition with ORlistat alleviated LPS-induced expression of IL-1β and IL-6. Specific inhibition of ATGL had a similar action on CCL2, IL-1β and IL-6 expression in both neonatal and adult microglia cultures. CCL2 and IL-6 secretion were also reduced by ATGListatin or knockdown of ATGL. ATGListatin increased phagocytosis in neonatal cultures independently from LPS treatment. Second, targeted and untargeted lipid profiling revealed that ATGListatin reduced LPS-induced generation of pro-inflammatory prostanoids and modulated ceramide species in neonatal microglia. Finally, the role of microglial ATGL in neuroinflammation was assessed using a novel microglia-specific and inducible ATGL knockout mouse model. Loss of microglial ATGL in adult male mice dampened LPS-induced expression of IL-6 and IL-1β and microglial density. LPS-induced sickness- and anxiety-like behaviours were also reduced in male mice with loss of ATGL in microglia. Together, our results demonstrate potent anti-inflammatory effects produced by pharmacological or genetic inhibition of ATGL-mediated triglyceride lipolysis and thereby propose that supressing microglial LD lipolysis has beneficial actions in acute neuroinflammatory conditions.
脂滴(LD)是储存甘油三酯的细胞器,已成为细胞炎症反应的重要组成部分。脂滴通过脂肪甘油三酯脂肪酶(ATGL)(一种催化甘油三酯脂肪分解限速步骤的酶)进行脂肪分解,从而调节外周免疫细胞和非免疫细胞的炎症反应。ATGL 以细胞类型依赖的方式在外周引起促炎和抗炎反应。本研究确定了抑制 ATGL 和小胶质细胞特异性 ATGL 基因功能缺失对急性炎症反应和对促炎症损伤的行为反应的影响。首先,我们评估了脂肪分解抑制对小鼠原代小胶质细胞培养物中脂多糖(LPS)诱导的细胞因子表达和分泌以及吞噬作用的影响。脂肪酶抑制剂(ORlistat 和 ATGListatin)和 LPS 会导致 LD 在小胶质细胞中积累。用ORlistat抑制泛脂肪酶可减轻LPS诱导的IL-1β和IL-6的表达。在新生儿和成年小胶质细胞培养物中,特异性抑制 ATGL 对 CCL2、IL-1β 和 IL-6 的表达也有类似作用。ATGListatin 或敲除 ATGL 也会减少 CCL2 和 IL-6 的分泌。ATGListatin 增加了新生儿培养物的吞噬能力,而与 LPS 处理无关。其次,靶向和非靶向脂质分析表明,ATGListatin 可减少 LPS 诱导的促炎前列腺素的生成,并调节新生小胶质细胞中的神经酰胺种类。最后,我们使用一种新型小胶质细胞特异性和诱导性 ATGL 基因敲除小鼠模型评估了小胶质细胞 ATGL 在神经炎症中的作用。成年雄性小鼠小胶质细胞 ATGL 的缺失抑制了 LPS 诱导的 IL-6 和 IL-1β 的表达以及小胶质细胞的密度。在小胶质细胞 ATGL 缺失的雄性小鼠中,LPS 诱导的病态和焦虑行为也有所减少。总之,我们的研究结果表明,通过药物或基因抑制 ATGL 介导的甘油三酯脂解作用可产生强大的抗炎效果,从而提出抑制小胶质细胞 LD 脂解作用对急性神经炎症有益处。
{"title":"Blockage of ATGL-mediated breakdown of lipid droplets in microglia alleviates neuroinflammatory and behavioural responses to lipopolysaccharides","authors":"Josephine Louise Robb ,&nbsp;Frédérick Boisjoly ,&nbsp;Arturo Israel Machuca-Parra ,&nbsp;Adeline Coursan ,&nbsp;Romane Manceau ,&nbsp;Danie Majeur ,&nbsp;Demetra Rodaros ,&nbsp;Khalil Bouyakdan ,&nbsp;Karine Greffard ,&nbsp;Jean-François Bilodeau ,&nbsp;Anik Forest ,&nbsp;Caroline Daneault ,&nbsp;Matthieu Ruiz ,&nbsp;Cyril Laurent ,&nbsp;Nathalie Arbour ,&nbsp;Sophie Layé ,&nbsp;Xavier Fioramonti ,&nbsp;Charlotte Madore ,&nbsp;Stephanie Fulton ,&nbsp;Thierry Alquier","doi":"10.1016/j.bbi.2024.09.027","DOIUrl":"10.1016/j.bbi.2024.09.027","url":null,"abstract":"<div><div>Lipid droplets (LD) are triglyceride storing organelles that have emerged as an important component of cellular inflammatory responses. LD lipolysis via adipose triglyceride lipase (ATGL), the enzyme that catalyses the rate-limiting step of triglyceride lipolysis, regulates inflammation in peripheral immune and non-immune cells. ATGL elicits both pro- and anti-inflammatory responses in the periphery in a cell-type dependent manner. The present study determined the impact of ATGL inhibition and microglia-specific ATGL genetic loss-of-function on acute inflammatory and behavioural responses to pro-inflammatory insult. First, we evaluated the impact of lipolysis inhibition on lipopolysaccharide (LPS)-induced expression and secretion of cytokines and phagocytosis in mouse primary microglia cultures. Lipase inhibitors (ORlistat and ATGListatin) and LPS led to LD accumulation in microglia. Pan-lipase inhibition with ORlistat alleviated LPS-induced expression of IL-1β and IL-6. Specific inhibition of ATGL had a similar action on CCL2, IL-1β and IL-6 expression in both neonatal and adult microglia cultures. CCL2 and IL-6 secretion were also reduced by ATGListatin or knockdown of ATGL. ATGListatin increased phagocytosis in neonatal cultures independently from LPS treatment. Second, targeted and untargeted lipid profiling revealed that ATGListatin reduced LPS-induced generation of pro-inflammatory prostanoids and modulated ceramide species in neonatal microglia. Finally, the role of microglial ATGL in neuroinflammation was assessed using a novel microglia-specific and inducible ATGL knockout mouse model. Loss of microglial ATGL in adult male mice dampened LPS-induced expression of IL-6 and IL-1β and microglial density. LPS-induced sickness- and anxiety-like behaviours were also reduced in male mice with loss of ATGL in microglia. Together, our results demonstrate potent anti-inflammatory effects produced by pharmacological or genetic inhibition of ATGL-mediated triglyceride lipolysis and thereby propose that supressing microglial LD lipolysis has beneficial actions in acute neuroinflammatory conditions.</div></div>","PeriodicalId":9199,"journal":{"name":"Brain, Behavior, and Immunity","volume":"123 ","pages":"Pages 315-333"},"PeriodicalIF":8.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Brain, Behavior, and Immunity
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