Pub Date : 2022-05-23DOI: 10.1186/s13058-022-01528-w
Ashvathi Raghavakaimal, Massimo Cristofanilli, Cha-Mei Tang, R K Alpaugh, Kirby P Gardner, Saranya Chumsri, Daniel L Adams
Background: CCR5 is a motility chemokine receptor implicated in tumor progression, whose activation and subsequent endocytosis may identify highly aggressive breast cancer cell subtypes likely to spread into the circulatory system.
Methods: The MDA-MB-231 cell line was used to model and visualize CCR5 activation by stimulation with RANTES, in an effort to quantify CCR5 endocytosis from the cell surface to the perinuclear space. CCR5 expression was then examined in tumor-associated cells (TACs), consisting of circulating tumor cells and circulating stromal cells, isolated from the peripheral blood of 54 metastatic breast cancer (mBC) patients to evaluate these CCR5 pooling patterns as they relate to progression and survival over 2 years.
Results: In MB231 experiments, it was observed that CCR5 formed ~ 1 micron clusters identified as "CCR5 pools" on the surface of the cell, which in the presence of RANTES were endocytosed and translocated to the cell cytoplasm. When TACs from patients were analyzed, CCR5 pools were observed on the cell surface and translocating to the nuclear area, with CCR5 also having a positive statistical correlation between increased numbers of TACs and increased CCR5 pools on the cells. Further, it was determined that patients with very high numbers of CCR5 (> 10 CCR5 pools), specifically in the circulating stromal cells, were associated with worse progression-free survival (hazard ratio = 4.5, p = 0.002) and worse overall survival (hazard ratio = 3.7, p = 0.014).
Conclusions: Using a liquid biopsy approach, we evaluated two populations of tumor-associated cells emanating from primary tumors, with data suggesting that upregulation of the motility chemokine CCR5 in TACs provides clinically relevant opportunities for treating and tracking drug targetable receptors in mBC.
{"title":"CCR5 activation and endocytosis in circulating tumor-derived cells isolated from the blood of breast cancer patients provide information about clinical outcome.","authors":"Ashvathi Raghavakaimal, Massimo Cristofanilli, Cha-Mei Tang, R K Alpaugh, Kirby P Gardner, Saranya Chumsri, Daniel L Adams","doi":"10.1186/s13058-022-01528-w","DOIUrl":"10.1186/s13058-022-01528-w","url":null,"abstract":"<p><strong>Background: </strong>CCR5 is a motility chemokine receptor implicated in tumor progression, whose activation and subsequent endocytosis may identify highly aggressive breast cancer cell subtypes likely to spread into the circulatory system.</p><p><strong>Methods: </strong>The MDA-MB-231 cell line was used to model and visualize CCR5 activation by stimulation with RANTES, in an effort to quantify CCR5 endocytosis from the cell surface to the perinuclear space. CCR5 expression was then examined in tumor-associated cells (TACs), consisting of circulating tumor cells and circulating stromal cells, isolated from the peripheral blood of 54 metastatic breast cancer (mBC) patients to evaluate these CCR5 pooling patterns as they relate to progression and survival over 2 years.</p><p><strong>Results: </strong>In MB231 experiments, it was observed that CCR5 formed ~ 1 micron clusters identified as \"CCR5 pools\" on the surface of the cell, which in the presence of RANTES were endocytosed and translocated to the cell cytoplasm. When TACs from patients were analyzed, CCR5 pools were observed on the cell surface and translocating to the nuclear area, with CCR5 also having a positive statistical correlation between increased numbers of TACs and increased CCR5 pools on the cells. Further, it was determined that patients with very high numbers of CCR5 (> 10 CCR5 pools), specifically in the circulating stromal cells, were associated with worse progression-free survival (hazard ratio = 4.5, p = 0.002) and worse overall survival (hazard ratio = 3.7, p = 0.014).</p><p><strong>Conclusions: </strong>Using a liquid biopsy approach, we evaluated two populations of tumor-associated cells emanating from primary tumors, with data suggesting that upregulation of the motility chemokine CCR5 in TACs provides clinically relevant opportunities for treating and tracking drug targetable receptors in mBC.</p>","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"24 1","pages":"35"},"PeriodicalIF":6.1,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65772635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.1186/s13058-022-01526-y
B. Rousseau, Sengottuvelan Murugan, Ajay Palagani, D. Sarkar
{"title":"Beta 2 adrenergic receptor and mu opioid receptor interact to potentiate the aggressiveness of human breast cancer cell by activating the glycogen synthase kinase 3 signaling","authors":"B. Rousseau, Sengottuvelan Murugan, Ajay Palagani, D. Sarkar","doi":"10.1186/s13058-022-01526-y","DOIUrl":"https://doi.org/10.1186/s13058-022-01526-y","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"39 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80577043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-09DOI: 10.1186/s13058-022-01527-x
Jieling Zheng, Bin Zhang
{"title":"Biological interpretation of prognostic radiomic score by correlating with tumor heterogeneity and microenvironment","authors":"Jieling Zheng, Bin Zhang","doi":"10.1186/s13058-022-01527-x","DOIUrl":"https://doi.org/10.1186/s13058-022-01527-x","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65772621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-03DOI: 10.1186/s13058-022-01525-z
W. Ho, Andrew M. K. Law, Etienne Masle-Farquhar, Lesley E Castillo, A. Mawson, Moira K. O’Bryan, C. Goodnow, D. Gallego-Ortega, S. Oakes, C. Ormandy
{"title":"Activation of the viral sensor oligoadenylate synthetase 2 (Oas2) prevents pregnancy-driven mammary cancer metastases","authors":"W. Ho, Andrew M. K. Law, Etienne Masle-Farquhar, Lesley E Castillo, A. Mawson, Moira K. O’Bryan, C. Goodnow, D. Gallego-Ortega, S. Oakes, C. Ormandy","doi":"10.1186/s13058-022-01525-z","DOIUrl":"https://doi.org/10.1186/s13058-022-01525-z","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":" ","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44167151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-14DOI: 10.1186/s13058-022-01522-2
Rachel L. Mintz, Mei Wang, Shuaili Xu, G. Colditz, C. Markovic, Adetunji T. Toriola
{"title":"Hormone and receptor activator of NF-κB (RANK) pathway gene expression in plasma and mammographic breast density in postmenopausal women","authors":"Rachel L. Mintz, Mei Wang, Shuaili Xu, G. Colditz, C. Markovic, Adetunji T. Toriola","doi":"10.1186/s13058-022-01522-2","DOIUrl":"https://doi.org/10.1186/s13058-022-01522-2","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80366287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-05DOI: 10.1186/s13058-022-01514-2
S. L. Foo, K. Sachaphibulkij, Corinne Lee, Gracemary L. R. Yap, Jianzhou Cui, T. Arumugam, L. Lim
{"title":"Breast cancer metastasis to brain results in recruitment and activation of microglia through annexin-A1/formyl peptide receptor signaling","authors":"S. L. Foo, K. Sachaphibulkij, Corinne Lee, Gracemary L. R. Yap, Jianzhou Cui, T. Arumugam, L. Lim","doi":"10.1186/s13058-022-01514-2","DOIUrl":"https://doi.org/10.1186/s13058-022-01514-2","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65772573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.1186/s13058-022-01518-y
Anne-Laure Renault, J. Dowty, Jason A. Steen, Shuai Li, I. Winship, G. Giles, J. Hopper, M. Southey, T. Nguyen-Dumont
{"title":"Population-based estimates of age-specific cumulative risk of breast cancer for pathogenic variants in ATM","authors":"Anne-Laure Renault, J. Dowty, Jason A. Steen, Shuai Li, I. Winship, G. Giles, J. Hopper, M. Southey, T. Nguyen-Dumont","doi":"10.1186/s13058-022-01518-y","DOIUrl":"https://doi.org/10.1186/s13058-022-01518-y","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65772614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.1186/s13058-022-01521-3
Guus J. J. E. Heynen, Kamil Lisek, Regina Vogel, A. Wulf‐Goldenberg, Joshua Alcaniz, Elodie Montaudon, E. Marangoni, W. Birchmeier
{"title":"Targeting SHP2 phosphatase in breast cancer overcomes RTK-mediated resistance to PI3K inhibitors","authors":"Guus J. J. E. Heynen, Kamil Lisek, Regina Vogel, A. Wulf‐Goldenberg, Joshua Alcaniz, Elodie Montaudon, E. Marangoni, W. Birchmeier","doi":"10.1186/s13058-022-01521-3","DOIUrl":"https://doi.org/10.1186/s13058-022-01521-3","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87336096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-21DOI: 10.1186/s13058-022-01519-x
H. Won, J. Ahn, Y. Kim, Jin Sung Kim, J. Song, Hong-Kyu Kim, Jeeyeon Lee, H. Park, Yong-Seok Kim
{"title":"Clinical significance of HER2-low expression in early breast cancer: a nationwide study from the Korean Breast Cancer Society","authors":"H. Won, J. Ahn, Y. Kim, Jin Sung Kim, J. Song, Hong-Kyu Kim, Jeeyeon Lee, H. Park, Yong-Seok Kim","doi":"10.1186/s13058-022-01519-x","DOIUrl":"https://doi.org/10.1186/s13058-022-01519-x","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"81 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88005552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Radiomics predicts the prognosis of patients with locally advanced breast cancer by reflecting the heterogeneity of tumor cells and the tumor microenvironment","authors":"Xuanyi Wang, Tiansong Xie, Ju-rui Luo, Zhengrong Zhou, Xiaoli Yu, Xiaomao Guo","doi":"10.1186/s13058-022-01516-0","DOIUrl":"https://doi.org/10.1186/s13058-022-01516-0","url":null,"abstract":"","PeriodicalId":9222,"journal":{"name":"Breast Cancer Research","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78756858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号