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Correction: Mcl-1 confers protection of Her2-positive breast cancer cells to hypoxia: therapeutic implications 更正:Mcl-1能保护Her2阳性乳腺癌细胞免受缺氧影响:治疗意义
IF 7.4 1区 医学 Pub Date : 2024-04-02 DOI: 10.1186/s13058-024-01811-y
Muhammad Hasan Bashari, Fengjuan Fan, Sonia Vallet, Martin Sattler, Melissa Arn, Claudia Luckner-Minden, Henning Schulze-Bergkamen, Inka Zörnig, Frederik Marme, Andreas Schneeweiss, Michael H. Cardone, Joseph T. Opferman, Dirk Jäger, Klaus Podar

Correction: Breast Cancer Research (2016) 18:26 https://doi.org/10.1186/s13058-016-0686-4

Following the publication of the original article [1], the author reported that PARP blots of JIMT-1 and JIMT-1-BR3 cells in Fig. 7C of the original publication were mistakenly duplicated.

Error image

figure a

The correct figure below depicts the correct PARP blot for JIMT-1 cells.

Correct image

figure b

The error does not affect any of the interpretations or conclusions of the article.

The email address of the Corresponding Author has also been updated, from klaus.podar@nct-heidelberg.de to klaus.podar@krems.lknoe.at as shown in this Correction article.

The original article [1] has been updated.

  1. Bashari MH, Fan F, Vallet S, et al. Mcl-1 confers protection of Her2-positive breast cancer cells to hypoxia: therapeutic implications. Breast Cancer Res. 2016;18:26. https://doi.org/10.1186/s13058-016-0686-4.

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Authors and Affiliations

  1. Department of Medical Oncology, National Center for Tumor Diseases (NCT), University of Heidelberg, Im Neuenheimer Feld #460, 69120, Heidelberg, Germany

    Muhammad Hasan Bashari, Fengjuan Fan, Sonia Vallet, Claudia Luckner-Minden, Henning Schulze-Bergkamen, Inka Zörnig, Frederik Marme, Andreas Schneeweiss, Dirk Jäger & Klaus Podar

  2. Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Padjadjaran, Jl. Eijkman 38, Bandung, 02215, Indonesia

    Muhammad Hasan Bashari

  3. Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02215, USA

    Martin Sattler

  4. Eutropics, Inc., 767C Concord Avenue, Cambridge, MA, 02138, USA

    Melissa Arn & Michael H. Cardone

  5. German Cancer Research Center (DKFZ), Im Neuenheimer Feld 460, 69120, Heidelberg, Germany

    Claudia Luckner-Minden, Inka Zörnig & Dirk Jäger

  6. St. Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, TN, 38105, USA

    Joseph T. Opferman

Authors
  1. Muhammad Hasan BashariView author public
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引用次数: 0
Factors associated with overall survival in breast cancer patients with leptomeningeal disease (LMD): a single institutional retrospective review 与伴有左侧脑膜疾病(LMD)的乳腺癌患者总生存率相关的因素:单个机构的回顾性研究
IF 7.4 1区 医学 Pub Date : 2024-03-29 DOI: 10.1186/s13058-024-01789-7
Gerald Wallace, Ronak Kundalia, Ethan Vallebuona, Biwei Cao, Youngchul Kim, Peter Forsyth, Aixa Soyano, Inna Smalley, Yolanda Pina
Breast cancer-related leptomeningeal disease (BC-LMD) is a dire diagnosis for 5–8% of patients with breast cancer (BC). We conducted a retrospective review of BC-LMD patients diagnosed at Moffitt Cancer Center from 2011 to 2020, to determine the changing incidence of BC-LMD, factors which are associated with the progression of BC CNS metastasis to BC-LMD, and factors which are associated with OS for patients with BC-LMD. Patients with BC and brain/spinal metastatic disease were identified. For those who eventually developed BC-LMD, we used Kaplan–Meier survival curve, log-rank test, univariable, and multivariate Cox proportional hazards regression model to identify factors affecting time from CNS metastasis to BC-LMD and OS. 128 cases of BC-LMD were identified. The proportion of BC-LMD to total BC patients was higher between 2016 and 2020 when compared to 2011–2015. Patients with HR+ or HER2 + BC experienced longer times between CNS metastasis and LMD than patients with triple-negative breast cancer (TNBC). Systemic therapy and whole-brain radiation therapy (WBRT) was associated with prolonged progression to LMD in all patients. Hormone therapy in patients with HR + BC were associated with a delayed BC-CNS metastasis to LMD progression. Lapatinib treatment was associated with a delayed progression to LMD in patients with HER2 + BC. Patients with TNBC-LMD had shorter OS compared to those with HR + and HER2 + BC-LMD. Systemic therapy, intrathecal (IT) therapy, and WBRT was associated with prolonged survival for all patients. Lapatinib and trastuzumab therapy was associated with improved OS in patients with HER2 + BC-LMD. Increasing rates of BC-LMD provide treatment challenges and opportunities for clinical trials. Prospective trials testing lapatinib and/or similar tyrosine kinase inhibitors, IT therapies, and combination treatments are urgently needed.
5%-8%的乳腺癌(BC)患者会被诊断为乳腺癌相关脑脊髓疾病(BC-LMD)。我们对 2011 年至 2020 年期间在莫菲特癌症中心确诊的 BC-LMD 患者进行了回顾性研究,以确定 BC-LMD 的发病率变化情况、与 BC 中枢神经系统转移发展为 BC-LMD 相关的因素,以及与 BC-LMD 患者 OS 相关的因素。研究人员对患有 BC 和脑/脊柱转移性疾病的患者进行了鉴定。对于最终发展为BC-LMD的患者,我们采用卡普兰-梅耶生存曲线、对数秩检验、单变量和多变量考克斯比例危险回归模型来确定影响从中枢神经系统转移到BC-LMD的时间和OS的因素。共发现128例BC-LMD病例。与2011-2015年相比,2016-2020年间BC-LMD占BC患者总数的比例更高。与三阴性乳腺癌(TNBC)患者相比,HR+或HER2 + BC患者从中枢神经系统转移到LMD的时间更长。全身治疗和全脑放疗 (WBRT) 与所有患者的 LMD 进展时间延长有关。HR+BC患者接受激素治疗与BC-CNS转移至LMD的进展延迟有关。拉帕替尼治疗与HER2+BC患者延迟进展至LMD有关。与HR +和HER2 + BC-LMD患者相比,TNBC-LMD患者的OS较短。全身治疗、鞘内治疗和WBRT与所有患者的生存期延长有关。拉帕替尼和曲妥珠单抗疗法可改善HER2 + BC-LMD患者的生存期。BC-LMD发病率的增加为临床试验带来了治疗挑战和机遇。目前急需开展前瞻性试验,测试拉帕替尼和/或类似的酪氨酸激酶抑制剂、IT疗法和联合疗法。
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引用次数: 0
Establishing conditions for the generation and maintenance of estrogen receptor-positive organoid models of breast cancer 建立生成和维持雌激素受体阳性乳腺癌类器官模型的条件
IF 7.4 1区 医学 Pub Date : 2024-03-29 DOI: 10.1186/s13058-024-01798-6
Michael U J Oliphant, Dipikaa Akshinthala, Senthil K. Muthuswamy
Patient-derived organoid models of estrogen receptor-positive (ER+) breast cancer would provide a much-needed tool to understand drug resistance and disease progression better. However, the establishment and long-term maintenance of ER expression, function, and response in vitro remains a significant challenge. Here, we report the development of an ER+ breast tumor organoid medium (BTOM-ER) that conserves ER expression, estrogen responsiveness, and dependence, as well as sensitivity to endocrine therapy of ER+ patient-derived xenograft organoids (PDXO). Our findings demonstrate the utility of subtype-specific culture conditions that better mimic the characteristics of the breast epithelial biology and microenvironment, providing a powerful platform for investigating therapy response and disease progression of ER+ breast cancer.
雌激素受体阳性(ER+)乳腺癌的患者衍生类器官模型将为更好地了解耐药性和疾病进展提供急需的工具。然而,如何在体外建立并长期保持ER的表达、功能和反应仍然是一项重大挑战。在这里,我们报告了一种ER+乳腺肿瘤类器官培养基(BTOM-ER)的开发情况,它能保持ER的表达、雌激素反应性和依赖性,以及对ER+患者异种移植类器官(PDXO)内分泌治疗的敏感性。我们的研究结果表明,亚型特异性培养条件能更好地模拟乳腺上皮生物学特性和微环境,为研究ER+乳腺癌的治疗反应和疾病进展提供了一个强大的平台。
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引用次数: 0
Paradoxical cancer cell proliferation after FGFR inhibition through decreased p21 signaling in FGFR1-amplified breast cancer cells 抑制表皮生长因子受体(FGFR)后,FGFR1 扩增的乳腺癌细胞通过减少 p21 信号传导出现癌细胞增殖的矛盾现象
IF 7.4 1区 医学 Pub Date : 2024-03-29 DOI: 10.1186/s13058-024-01808-7
Feng Chi, Jason I. Griffiths, Aritro Nath, Andrea H. Bild
Fibroblast growth factors (FGFs) control various cellular functions through fibroblast growth factor receptor (FGFR) activation, including proliferation, differentiation, migration, and survival. FGFR amplification in ER + breast cancer patients correlate with poor prognosis, and FGFR inhibitors are currently being tested in clinical trials. By comparing three-dimensional spheroid growth of ER + breast cancer cells with and without FGFR1 amplification, our research discovered that FGF2 treatment can paradoxically decrease proliferation in cells with FGFR1 amplification or overexpression. In contrast, FGF2 treatment in cells without FGFR1 amplification promotes classical FGFR proliferative signaling through the MAPK cascade. The growth inhibitory effect of FGF2 in FGFR1 amplified cells aligned with an increase in p21, a cell cycle inhibitor that hinders the G1 to S phase transition in the cell cycle. Additionally, FGF2 addition in FGFR1 amplified cells activated JAK-STAT signaling and promoted a stem cell-like state. FGF2-induced paradoxical effects were reversed by inhibiting p21 or the JAK-STAT pathway and with pan-FGFR inhibitors. Analysis of patient ER + breast tumor transcriptomes from the TCGA and METABRIC datasets demonstrated a strong positive association between expression of FGF2 and stemness signatures, which was further enhanced in tumors with high FGFR1 expression. Overall, our findings reveal a divergence in FGFR signaling, transitioning from a proliferative to stemness state driven by activation of JAK-STAT signaling and modulation of p21 levels. Activation of these divergent signaling pathways in FGFR amplified cancer cells and paradoxical growth effects highlight a challenge in the use of FGFR inhibitors in cancer treatment.
成纤维细胞生长因子(FGF)通过激活成纤维细胞生长因子受体(FGFR)控制各种细胞功能,包括增殖、分化、迁移和存活。ER+乳腺癌患者的FGFR扩增与预后不良有关,FGFR抑制剂目前正在进行临床试验。通过比较有无 FGFR1 扩增的 ER + 乳腺癌细胞的三维球状生长,我们的研究发现,FGF2 治疗可降低 FGFR1 扩增或过表达细胞的增殖。相反,在没有 FGFR1 扩增的细胞中处理 FGF2 会通过 MAPK 级联促进经典的 FGFR 增殖信号传导。FGF2 对 FGFR1 扩增细胞的生长抑制作用与细胞周期抑制剂 p21 的增加相一致,p21 会阻碍细胞周期中 G1 期向 S 期的转变。此外,在FGFR1扩增细胞中添加FGF2激活了JAK-STAT信号,促进了干细胞样状态。抑制p21或JAK-STAT通路以及使用泛FGFR抑制剂可逆转FGF2诱导的矛盾效应。对来自TCGA和METABRIC数据集的患者ER +乳腺肿瘤转录组的分析表明,FGF2的表达与干性特征之间存在很强的正相关性,在FGFR1高表达的肿瘤中,这种正相关性进一步增强。总之,我们的研究结果揭示了FGFR信号转导的分化,即在JAK-STAT信号转导激活和p21水平调节的驱动下,从增殖状态过渡到干性状态。FGFR扩增癌细胞中这些不同信号通路的激活以及矛盾的生长效应凸显了在癌症治疗中使用FGFR抑制剂所面临的挑战。
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引用次数: 0
TMEM120B strengthens breast cancer cell stemness and accelerates chemotherapy resistance via β1-integrin/FAK-TAZ-mTOR signaling axis by binding to MYH9 TMEM120B与MYH9结合,通过β1-整合素/FAK-TAZ-mTOR信号轴增强乳腺癌细胞的干性并加速化疗耐受性
IF 7.4 1区 医学 Pub Date : 2024-03-19 DOI: 10.1186/s13058-024-01802-z
Ran Hu, Yu Cao, Yuanyuan Wang, Tingting Zhao, Kaibo Yang, Mingwei Fan, Mengyao Guan, Yuekang Hou, Jiao Ying, Xiaowen Ma, Ning Deng, Xun Sun, Yong Zhang, Xiupeng Zhang
Breast cancer stem cell (CSC) expansion results in tumor progression and chemoresistance; however, the modulation of CSC pluripotency remains unexplored. Transmembrane protein 120B (TMEM120B) is a newly discovered protein expressed in human tissues, especially in malignant tissues; however, its role in CSC expansion has not been studied. This study aimed to determine the role of TMEM120B in transcriptional coactivator with PDZ-binding motif (TAZ)-mediated CSC expansion and chemotherapy resistance. Both bioinformatics analysis and immunohistochemistry assays were performed to examine expression patterns of TMEM120B in lung, breast, gastric, colon, and ovarian cancers. Clinicopathological factors and overall survival were also evaluated. Next, colony formation assay, MTT assay, EdU assay, transwell assay, wound healing assay, flow cytometric analysis, sphere formation assay, western blotting analysis, mouse xenograft model analysis, RNA-sequencing assay, immunofluorescence assay, and reverse transcriptase-polymerase chain reaction were performed to investigate the effect of TMEM120B interaction on proliferation, invasion, stemness, chemotherapy sensitivity, and integrin/FAK/TAZ/mTOR activation. Further, liquid chromatography–tandem mass spectrometry analysis, GST pull-down assay, and immunoprecipitation assays were performed to evaluate the interactions between TMEM120B, myosin heavy chain 9 (MYH9), and CUL9. TMEM120B expression was elevated in lung, breast, gastric, colon, and ovarian cancers. TMEM120B expression positively correlated with advanced TNM stage, lymph node metastasis, and poor prognosis. Overexpression of TMEM120B promoted breast cancer cell proliferation, invasion, and stemness by activating TAZ-mTOR signaling. TMEM120B directly bound to the coil-coil domain of MYH9, which accelerated the assembly of focal adhesions (FAs) and facilitated the translocation of TAZ. Furthermore, TMEM120B stabilized MYH9 by preventing its degradation by CUL9 in a ubiquitin-dependent manner. Overexpression of TMEM120B enhanced resistance to docetaxel and doxorubicin. Conversely, overexpression of TMEM120B-∆CCD delayed the formation of FAs, suppressed TAZ-mTOR signaling, and abrogated chemotherapy resistance. TMEM120B expression was elevated in breast cancer patients with poor treatment outcomes (Miller/Payne grades 1–2) than in those with better outcomes (Miller/Payne grades 3–5). Our study reveals that TMEM120B bound to and stabilized MYH9 by preventing its degradation. This interaction activated the β1-integrin/FAK-TAZ-mTOR signaling axis, maintaining stemness and accelerating chemotherapy resistance.
乳腺癌干细胞(CSC)扩增会导致肿瘤进展和化疗耐药性;然而,对CSC多能性的调控仍有待探索。跨膜蛋白120B(TMEM120B)是一种新发现的在人体组织中表达的蛋白,尤其是在恶性组织中;然而,它在CSC扩增中的作用尚未被研究。本研究旨在确定TMEM120B在具有PDZ结合基调的转录辅激活因子(TAZ)介导的CSC扩增和化疗耐药中的作用。研究人员通过生物信息学分析和免疫组化检测,研究了TMEM120B在肺癌、乳腺癌、胃癌、结肠癌和卵巢癌中的表达模式。此外,还对临床病理因素和总生存率进行了评估。接着,研究人员进行了菌落形成试验、MTT试验、EdU试验、transwell试验、伤口愈合试验、流式细胞分析、球体形成试验、Western印迹分析、小鼠异种移植模型分析、RNA测序试验、免疫荧光试验和反转录聚合酶链反应,以研究TMEM120B相互作用对增殖、侵袭、干性、化疗敏感性和整合素/FAK/TAZ/mTOR激活的影响。此外,还进行了液相色谱-串联质谱分析、GST牵引试验和免疫沉淀试验,以评估TMEM120B、肌球蛋白重链9(MYH9)和CUL9之间的相互作用。TMEM120B在肺癌、乳腺癌、胃癌、结肠癌和卵巢癌中表达升高。TMEM120B的表达与TNM分期晚期、淋巴结转移和预后不良呈正相关。TMEM120B的过表达通过激活TAZ-mTOR信号传导促进了乳腺癌细胞的增殖、侵袭和干性。TMEM120B直接与MYH9的线圈盘旋结构域结合,从而加速了焦点粘附(FA)的组装,并促进了TAZ的转位。此外,TMEM120B还以泛素依赖的方式阻止MYH9被CUL9降解,从而稳定了MYH9。过表达 TMEM120B 会增强对多西他赛和多柔比星的耐药性。相反,TMEM120B-∆CCD的过表达会延迟FA的形成,抑制TAZ-mTOR信号转导,减轻化疗耐药性。治疗效果不佳的乳腺癌患者(Miller/Payne 分级 1-2)的 TMEM120B 表达高于治疗效果较好(Miller/Payne 分级 3-5)的患者。我们的研究发现,TMEM120B 与 MYH9 结合并阻止其降解,从而稳定了 MYH9。这种相互作用激活了β1-整合素/FAK-TAZ-mTOR信号轴,维持了干性并加速了化疗耐药性。
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引用次数: 0
The role of surgical tissue injury and intraoperative sympathetic activation in postoperative immunosuppression after breast-conserving surgery versus mastectomy: a prospective observational study 手术组织损伤和术中交感神经激活在保乳手术与乳房切除术后免疫抑制中的作用:一项前瞻性观察研究
IF 7.4 1区 医学 Pub Date : 2024-03-11 DOI: 10.1186/s13058-024-01801-0
Lotte MC Jacobs, Leonie S Helder, Kim I Albers, Josephine Kranendonk, Christiaan Keijzer, Leo AB Joosten, Luc JA Strobbe, Michiel C Warlé
Breast cancer is the second most common cause of death from cancer in women worldwide. Counterintuitively, large population-based retrospective trials report better survival after breast-conserving surgery (BCS) compared to mastectomy, corrected for tumour- and patient variables. More extensive surgical tissue injury and activation of the sympathetic nervous system by nociceptive stimuli are associated with immune suppression. We hypothesized that mastectomy causes a higher expression of plasma damage associated molecular patterns (DAMPs) and more intraoperative sympathetic activation which induce postoperative immune dysregulation. Immune suppression can lead to postoperative complications and affect tumour-free survival. In this prospective observational study, plasma DAMPs (HMGB1, HSP70, S100A8/A9 and S100A12), intraoperative sympathetic activation (Nociception Level (NOL) index from 0 to 100), and postoperative immune function (plasma cytokine concentrations and ex vivo cytokine production capacity) were compared in patients undergoing elective BCS (n = 20) versus mastectomy (n = 20). Ex vivo cytokine production capacity of TNF, IL-6 and IL-1β was nearly absent in both groups one hour after surgery. Levels appeared recovered on postoperative day 3 (POD3), with significantly higher ex vivo production capacity of IL-1β after BCS (p = .041) compared to mastectomy. Plasma concentration of IL-6 was higher one hour after mastectomy (p = .045). Concentrations of plasma alarmins S100A8/A9 and S100A12 were significantly higher on POD3 after mastectomy (p = .003 and p = .041, respectively). Regression analysis showed a significantly lower percentage of NOL measurements ≤ 8 (absence of nociception) during mastectomy when corrected for norepinephrine equivalents (36% versus 45% respectively, p = .038). Percentage of NOL measurements ≤ 8 of all patients correlated with ex vivo cytokine production capacity of IL-1β and TNF on POD3 (r = .408; p = .011 and r = .500; p = .001, respectively). This pilot study revealed substantial early postoperative immune suppression after BCS and mastectomy that appears to recover in the following days. Differences between BCS and mastectomy in release of DAMPs and intraoperative sympathetic activation could affect postoperative immune homeostasis and thereby contribute to the better survival reported after BCS in previous large population-based retrospective trials. These results endorse further exploration of (1) S100 alarmins as potential therapeutic targets in breast cancer surgery and (2) suppression of intraoperative sympathetic activation to substantiate the observed association with postoperative immune dysregulation.
乳腺癌是全球妇女死于癌症的第二大常见病因。与直觉相反的是,基于人群的大型回顾性试验报告显示,在对肿瘤和患者变量进行校正后,保乳手术(BCS)的存活率高于乳房切除术。更广泛的手术组织损伤以及交感神经系统在痛觉刺激下的激活与免疫抑制有关。我们假设乳房切除术会导致血浆损伤相关分子模式(DAMPs)的更高表达和术中交感神经的更多激活,从而诱发术后免疫失调。免疫抑制可导致术后并发症并影响无瘤生存。在这项前瞻性观察研究中,比较了接受择期乳腺切除术(BCS)(20 人)和乳腺切除术(20 人)患者的血浆 DAMPs(HMGB1、HSP70、S100A8/A9 和 S100A12)、术中交感神经激活(Nociception Level (NOL) index,从 0 到 100)和术后免疫功能(血浆细胞因子浓度和体内外细胞因子生成能力)。术后一小时,两组患者体内TNF、IL-6和IL-1β的体外细胞因子生成能力几乎都不存在。术后第 3 天(POD3),TNF、IL-6 和 IL-1β 的水平出现恢复,与乳房切除术相比,BCS 术后 IL-1β 的体内外产生能力明显更高(p = .041)。乳房切除术后一小时,血浆中的 IL-6 浓度更高(p = 045)。乳房切除术后 POD3 的血浆抗原 S100A8/A9 和 S100A12 浓度明显更高(分别为 p = .003 和 p = .041)。回归分析表明,在乳房切除术期间,经去甲肾上腺素当量校正后,NOL 测量值≤ 8(无痛觉)的百分比明显降低(分别为 36% 和 45%,p = .038)。所有患者的 NOL 测量值≤ 8 的百分比与 POD3 上 IL-1β 和 TNF 的体外细胞因子生成能力相关(r = .408; p = .011 和 r = .500; p = .001)。这项试点研究显示,乳房局部切除术和乳房切除术后的早期免疫抑制很严重,但在随后几天似乎会恢复。乳房局部切除术和乳房切除术在 DAMPs 释放和术中交感神经激活方面的差异可能会影响术后免疫平衡,从而导致之前基于人群的大型回顾性试验中报告的乳房局部切除术后较好的存活率。这些结果支持进一步探索:(1) 将 S100 alarmins 作为乳腺癌手术的潜在治疗靶点;(2) 抑制术中交感神经激活,以证实观察到的与术后免疫失调的关联。
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引用次数: 0
HER2-low and tumor infiltrating lymphocytes in triple-negative breast cancer: Are they connected? 三阴性乳腺癌中的 HER2 低表达和肿瘤浸润淋巴细胞:它们之间有联系吗?
IF 7.4 1区 医学 Pub Date : 2024-03-11 DOI: 10.1186/s13058-024-01783-z
Ximena Baez-Navarro, Nadine S. van den Ende, Anh H. Nguyen, Renata Sinke, Pieter Westenend, Johannes Bastiaan van Brakel, Claudia Stobbe, Johan Westerga, Carolien H. M. van Deurzen
Most patients with triple-negative breast cancer (TNBC) are not candidates for targeted therapy, leaving chemotherapy as the primary treatment option. Recently, immunotherapy has demonstrated promising results in TNBC, due to its immunogenicity. In addition, a novel antibody–drug conjugate, namely, trastuzumab-deruxtecan, has shown effectiveness in TNBC patients with low-HER2 expression (HER2-low). These novel treatment options raise the question about the potential association between the density of stromal tumor-infiltrating lymphocytes (sTILs) and the level of HER2 expression. We aimed to evaluate the association between the level of HER2 expression (HER2-low versus HER2-0) and density of sTILs in TNBC patients, and how they impact the response to neoadjuvant chemotherapy (NAC). This was a retrospective multicenter study including all TNBC patients diagnosed between 2018 and 2022. Central pathology review included sTILs percentages and level of HER2 expression. Tumors were reclassified as either HER2-0 (HER2 IHC 0) or HER2-low (IHC 1 + or 2 + with negative reflex test). Various clinicopathologic characteristics, including sTILs density, and response to NAC were compared between HER2-0 and HER2-low cases. In total, 753 TNBC patients were included in this study, of which 292 patients received NAC. Interobserver agreement between the original pathology report and central review was moderate (77% had the same IHC status after reclassification in either HER2-0 or HER2-low; k = 0.45). HER2-low TNBC represented about one third (36%) of the tumors. No significant difference in sTILs density or complete pathologic response rate was found between HER2-0 and HER2-low cases (p = 0.476 and p = 0.339, respectively). The density of sTILs (≥ 10% sTILs vs. < 10%) was independently associated with achieving a pCR (p = 0.011). In conclusion, no significant association was found between HER2-low status and density of sTILs nor response to NAC. Nonetheless, sTILs could be an independent biomarker for predicting NAC response in TNBC patients.
大多数三阴性乳腺癌(TNBC)患者不适合接受靶向治疗,因此化疗成为主要的治疗选择。最近,免疫疗法因其免疫原性在 TNBC 中取得了可喜的成果。此外,一种新型抗体-药物共轭物,即曲妥珠单抗-德鲁司坦,也显示出对HER2低表达(HER2-low)的TNBC患者有效。这些新型治疗方案提出了一个问题:基质肿瘤浸润淋巴细胞(sTILs)的密度与HER2表达水平之间可能存在关联。我们的目的是评估 TNBC 患者的 HER2 表达水平(HER2-low 与 HER2-0)和 sTILs 密度之间的关联,以及它们对新辅助化疗 (NAC) 反应的影响。这是一项回顾性多中心研究,包括2018年至2022年期间确诊的所有TNBC患者。中央病理审查包括 sTILs 百分比和 HER2 表达水平。肿瘤被重新分类为HER2-0(HER2 IHC 0)或HER2-低(IHC 1 +或2 +,反射试验阴性)。比较了 HER2-0 和 HER2 低病例的各种临床病理特征,包括 sTILs 密度和对 NAC 的反应。本研究共纳入了 753 例 TNBC 患者,其中 292 例患者接受了 NAC 治疗。原始病理报告与中央复查之间的观察者间一致性为中等(77%的患者在重新分类为HER2-0或HER2-low后具有相同的IHC状态;k = 0.45)。HER2低的TNBC约占肿瘤的三分之一(36%)。HER2-0和HER2-low病例的sTILs密度和完全病理反应率无明显差异(分别为p = 0.476和p = 0.339)。sTILs的密度(≥10% sTILs vs. <10%)与获得pCR独立相关(p = 0.011)。总之,HER2-low状态与sTILs密度或对NAC的反应均无明显关联。然而,sTILs可能是预测TNBC患者NAC反应的独立生物标志物。
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引用次数: 0
The FBXW7-binding sites on FAM83D are potential targets for cancer therapy FAM83D 上的 FBXW7 结合位点是癌症治疗的潜在靶点
IF 7.4 1区 医学 Pub Date : 2024-03-07 DOI: 10.1186/s13058-024-01795-9
Xiaoyu Jiang, Yuli Wang, Lulu Guo, Yige Wang, Tianshu Miao, Lijuan Ma, Qin Wei, Xiaoyan Lin, Jian-Hua Mao, Pengju Zhang
Increasing evidence shows the oncogenic function of FAM83D in human cancer, but how FAM83D exerts its oncogenic function remains largely unclear. Here, we investigated the importance of FAM83D/FBXW7 interaction in breast cancer (BC). We systematically mapped the FBXW7-binding sites on FAM83D through a comprehensive mutational analysis together with co-immunoprecipitation assay. Mutations at the FBXW7-binding sites on FAM83D led to that FAM83D lost its capability to promote the ubiquitination and proteasomal degradation of FBXW7; cell proliferation, migration, and invasion in vitro; and tumor growth and metastasis in vivo, indicating that the FBXW7-binding sites on FAM83D are essential for its oncogenic functions. A meta-evaluation of FAM83D revealed that the prognostic impact of FAM83D was independent on molecular subtypes. The higher expression of FAM83D has poorer prognosis. Moreover, high expression of FAM83D confers resistance to chemotherapy in BCs, which is experimentally validated in vitro. We conclude that identification of FBXW7-binding sites on FAM83D not only reveals the importance for FAM83D oncogenic function, but also provides valuable insights for drug target.
越来越多的证据表明,FAM83D在人类癌症中具有致癌功能,但FAM83D如何发挥其致癌功能在很大程度上仍不清楚。在此,我们研究了FAM83D/FBXW7相互作用在乳腺癌(BC)中的重要性。我们通过全面的突变分析和共沉淀实验系统地绘制了FAM83D上的FBXW7结合位点。FAM83D上的FBXW7结合位点突变导致FAM83D失去了促进FBXW7泛素化和蛋白酶体降解的能力;失去了体外促进细胞增殖、迁移和侵袭的能力;失去了体内促进肿瘤生长和转移的能力,这表明FAM83D上的FBXW7结合位点对其致癌功能至关重要。对 FAM83D 的荟萃评估显示,FAM83D 对预后的影响与分子亚型无关。FAM83D表达越高,预后越差。此外,FAM83D的高表达会使BC对化疗产生耐药性,这在体外实验中得到了验证。我们的结论是,FAM83D上FBXW7结合位点的鉴定不仅揭示了FAM83D致癌功能的重要性,还为药物靶向提供了有价值的见解。
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引用次数: 0
How does weight gain since the age of 18 years affect breast cancer risk in later life? A meta-analysis 18 岁以后的体重增加如何影响日后患乳腺癌的风险?荟萃分析
IF 7.4 1区 医学 Pub Date : 2024-03-07 DOI: 10.1186/s13058-024-01804-x
Yunan Han, Ebunoluwa E. Otegbeye, Carrie Stoll, Angela Hardi, Graham A. Colditz, Adetunji T. Toriola
Early life factors are important risk factors for breast cancer. The association between weight gain after age 18 and breast cancer risk is inconsistent across previous epidemiologic studies. To evaluate this association, we conducted a meta-analysis according to PRISMA guidelines and the established inclusion criteria. We performed a comprehensive literature search using Medline (Ovid), Embase, Scopus, Cochrane Library, and ClinicalTrials.gov to identify relevant studies published before June 3, 2022. Two reviewers independently reviewed the articles for final inclusion. Seventeen out of 4,725 unique studies met the selection criteria. The quality of studies was assessed using the Newcastle-Ottawa Scale (NOS), and all were of moderate to high quality with NOS scores ranging from 5 to 8. We included 17 studies (11 case-control, 6 cohort) in final analysis. In case-control studies, weight gain after age 18 was associated with an increased risk of breast cancer (odds ratio [OR] = 1.25; 95% CI = 1.07–1.48), when comparing the highest versus the lowest categories of weight gain. Menopausal status was a source of heterogeneity, with weight gain after age 18 associated with an increased risk of breast cancer in postmenopausal women (OR = 1.53; 95% CI = 1.40–1.68), but not in premenopausal women (OR = 1.01; 95% CI = 0.92–1.12). Additionally, a 5 kg increase in weight was positively associated with postmenopausal breast cancer risk (OR = 1.12; 95%CI = 1.05–1.21) in case-control studies. Findings from cohort studies were identical, with a positive association between weight gain after age 18 and breast cancer incidence in postmenopausal women (relative risk [RR] = 1.30; 95% CI = 1.09–1.36), but not in premenopausal women (RR = 1.06; 95% CI = 0.92–1.22). Weight gain after age 18 is a risk factor for postmenopausal breast cancer, highlighting the importance of weight control from early adulthood to reduce the incidence of postmenopausal breast cancer.
早期生活因素是乳腺癌的重要风险因素。在以往的流行病学研究中,18 岁以后体重增加与乳腺癌风险之间的关系并不一致。为了评估这种关联,我们根据 PRISMA 指南和既定的纳入标准进行了一项荟萃分析。我们使用 Medline (Ovid)、Embase、Scopus、Cochrane Library 和 ClinicalTrials.gov 进行了全面的文献检索,以确定 2022 年 6 月 3 日之前发表的相关研究。两名审稿人分别独立审阅文章,以确定最终纳入的研究。在 4725 项独特的研究中,有 17 项符合筛选标准。研究质量采用纽卡斯尔-渥太华量表(Newcastle-Ottawa Scale,NOS)进行评估,所有研究的质量均为中上等,NOS评分在5到8分之间。我们在最终分析中纳入了 17 项研究(11 项病例对照研究和 6 项队列研究)。在病例对照研究中,比较体重增加的最高和最低类别,18 岁以后体重增加与乳腺癌风险增加有关(几率比 [OR] = 1.25;95% CI = 1.07-1.48)。绝经状态是异质性的一个来源,绝经后妇女 18 岁后体重增加与乳腺癌风险增加有关(OR = 1.53;95% CI = 1.40-1.68),而绝经前妇女则无关(OR = 1.01;95% CI = 0.92-1.12)。此外,在病例对照研究中,体重增加 5 千克与绝经后乳腺癌风险呈正相关(OR = 1.12;95%CI = 1.05-1.21)。队列研究的结果相同,18 岁以后体重增加与绝经后妇女乳腺癌发病率呈正相关(相对风险 [RR] = 1.30;95% CI = 1.09-1.36),但与绝经前妇女无关(RR = 1.06;95% CI = 0.92-1.22)。18岁以后体重增加是绝经后乳腺癌的一个风险因素,这凸显了从成年早期开始控制体重对降低绝经后乳腺癌发病率的重要性。
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引用次数: 0
Clinically relevant gene signatures provide independent prognostic information in older breast cancer patients 临床相关基因特征为老年乳腺癌患者提供独立的预后信息
IF 7.4 1区 医学 Pub Date : 2024-03-07 DOI: 10.1186/s13058-024-01797-7
Miguel Castresana-Aguirre, Annelie Johansson, Alexios Matikas, Theodoros Foukakis, Linda S. Lindström, Nicholas P. Tobin
The clinical utility of gene signatures in older breast cancer patients remains unclear. We aimed to determine signature prognostic capacity in this patient subgroup. Research versions of the genomic grade index (GGI), 70-gene, recurrence score (RS), cell cycle score (CCS), PAM50 risk-of-recurrence proliferation (ROR-P), and PAM50 signatures were applied to 39 breast cancer datasets (N = 9583). After filtering on age ≥ 70 years, and the presence of estrogen receptor (ER) and survival data, 871 patients remained. Signature prognostic capacity was tested in all (n = 871), ER-positive/lymph node-positive (ER + /LN + , n = 335) and ER-positive/lymph node-negative (ER + /LN−, n = 374) patients using Kaplan–Meier and multivariable Cox-proportional hazard (PH) modelling. All signatures were statistically significant in Kaplan–Meier analysis of all patients (Log-rank P < 0.001). This significance remained in multivariable analysis (Cox-PH, P ≤ 0.05). In ER + /LN + patients all signatures except PAM50 were significant in Kaplan–Meier analysis (Log-rank P ≤ 0.05) and remained so in multivariable analysis (Cox-PH, P ≤ 0.05). In ER + /LN− patients all except RS were significant in Kaplan–Meier analysis (Log-rank P ≤ 0.05) but only the 70-gene, CCS, ROR-P, and PAM50 signatures remained so in multivariable analysis (Cox-PH, P ≤ 0.05). We found that gene signatures provide prognostic information in survival analyses of all, ER + /LN + and ER + /LN- older (≥ 70 years) breast cancer patients, suggesting a potential role in aiding treatment decisions in older patients.
基因特征在老年乳腺癌患者中的临床应用仍不明确。我们的目的是确定这一患者亚群的特征预后能力。我们将基因组分级指数(GGI)、70-基因、复发评分(RS)、细胞周期评分(CCS)、PAM50 复发风险增殖(ROR-P)和 PAM50 签名的研究版本应用于 39 个乳腺癌数据集(N = 9583)。在筛选出年龄≥70岁、有雌激素受体(ER)和生存数据的患者后,剩下871名患者。使用 Kaplan-Meier 和多变量 Cox 比例危险(PH)建模法对所有患者(n = 871)、ER 阳性/淋巴结阳性(ER + /LN +,n = 335)和ER 阳性/淋巴结阴性(ER + /LN-,n = 374)患者的特征预后能力进行了检测。在对所有患者进行卡普兰-梅耶分析时,所有特征均具有统计学意义(Log-rank P < 0.001)。这种显著性在多变量分析中依然存在(Cox-PH,P ≤ 0.05)。在 ER + /LN + 患者中,除 PAM50 外,所有特征在 Kaplan-Meier 分析中均具有显著性(Log-rank P ≤ 0.05),在多变量分析中仍具有显著性(Cox-PH,P ≤ 0.05)。在ER + /LN-患者中,除RS外,其他基因在卡普兰-梅耶分析中均有显著性(Log-rank P≤0.05),但只有70基因、CCS、ROR-P和PAM50特征在多变量分析中仍有显著性(Cox-PH,P≤0.05)。我们发现,基因特征在所有、ER + /LN + 和 ER + /LN- 老年(≥ 70 岁)乳腺癌患者的生存分析中提供了预后信息,这表明基因特征在帮助老年患者做出治疗决定方面具有潜在的作用。
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Breast Cancer Research
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