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Impact of DNA Extraction Methods on Quantitative PCR Telomere Length Assay Precision in Human Saliva Samples DNA提取方法对人唾液样品定量PCR端粒长度测定精度的影响
Pub Date : 2022-11-27 DOI: 10.21467/ijm.1.1.5784
Dana L. Smith, Calvin Wu, S. Gregorich, Guorui Dai, Jue Lin
Telomere length (TL) has emerged as a promising replicative cellular aging marker that reflects both genetic and non-genetic influences. Quantitative PCR (qPCR) TL measurement has been favored as a cost-effective method that can be easily implemented, especially in population studies with limited quantities of source material. However, several recent reports have revealed inconsistencies in telomere length measurements when applying different DNA extraction methods to the same source material. In this study we tested three DNA extraction methods on saliva samples from 48 participants of the National Growth and Health Study (NGHS) collected with DNA Genotek’s Oragene kit. The chosen extraction kits represent three distinct approaches to genomic DNA extraction from lysed cells and we employed two different operators to carry out all assays on the same samples. We measured DNA yield and quality and calculated the between-operator agreement of qPCR TL measurements (intraclass correlation, ICC). Our analyses showed that while both QIAamp and Agencourt DNAdvance had higher agreement between the 2 operators (ICC=0.937, CI [0.891, 0.965] and ICC=0.95, CI [0.911, 0.972] respectively), compared to PrepIT kit (ICC=0.809, CI [0.678, 0.889]), QIAamp extracted DNA samples were notably degraded. Using generalizability theory, we found that the participant-by-extraction-method interaction explained about 10% of total variation in TL, suggesting that TL differences across methods are somewhat participant-specific. Therefore, our results suggest that the among the three DNA extraction methods tested, Agencourt (magnetic bead purification) is the preferred kit, and we also strongly recommend against combining different extraction methods within a study population.
端粒长度(TL)作为一种反映遗传和非遗传影响的有希望的复制细胞衰老标志物已经出现。定量PCR (qPCR) TL测量作为一种成本效益高、易于实施的方法而受到青睐,特别是在源材料数量有限的种群研究中。然而,最近的一些报告揭示了端粒长度测量的不一致性,当应用不同的DNA提取方法对同一来源材料。在这项研究中,我们测试了三种DNA提取方法对48名国家生长与健康研究(NGHS)参与者的唾液样本进行提取,这些唾液样本是用DNA Genotek的Oragene试剂盒收集的。所选择的提取试剂盒代表了从裂解细胞中提取基因组DNA的三种不同方法,我们采用了两种不同的操作人员对同一样品进行所有检测。我们测量了DNA产量和质量,并计算了qPCR TL测量的算子间一致性(类内相关性,ICC)。我们的分析表明,虽然QIAamp和agcourt DNAdvance两种操作器之间的一致性更高(ICC=0.937, CI[0.891, 0.965]和ICC=0.95, CI[0.911, 0.972]),但与PrepIT试剂盒(ICC=0.809, CI[0.678, 0.889])相比,QIAamp提取的DNA样本明显降解。利用概化理论,我们发现参与者与提取方法之间的相互作用解释了大约10%的TL总变异,这表明不同方法之间的TL差异在某种程度上是参与者特有的。因此,我们的结果表明,在测试的三种DNA提取方法中,agcourt(磁珠纯化)是首选试剂盒,我们也强烈建议不要在研究人群中使用不同的提取方法。
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引用次数: 1
Psychometric Properties of Online Adolescent Anger Instrument 在线青少年愤怒量表的心理测量特性
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.819
Nor Shafrin, Rozniza Zaharudin, A. Zamri
Anger is a topic that requires intervention from teachers, counsellors, psychologists, parents, and all communities. The expressions of anger are subjective and sometimes hard to identify. Thus, anger should be measured more objectively, while the expressions need to be examined closely. The purpose of this study is to provide valid confirmation for development of an online instrument to measure the types of anger expression among adolescents. Data were collected from 935 adolescents from nine schools in northern Malaysia and the theoretical literature search. The data were analysed to provide evidence of construct validity in terms of item factor analysis, reliability estimates, and correlation between the types of anger expressions. Findings were used to develop an online Adolescent Anger Instrument. It measures five types of anger expressions, namely, physical, verbal, intrinsic, extrinsic, and passive. The results showed that the instrument is internally consistent with high evidence of construct validity. Exploratory factor analysis, with varimax rotation, suggested the existence of five distinct types of anger as conceptualised. Meanwhile, the correlation between types of anger expressions indicates the strength of the relationship between them. Discussions on the findings are provided, while suggestions for future research are also described.
愤怒是一个需要教师、咨询师、心理学家、家长和所有社区介入的话题。愤怒的表达是主观的,有时很难识别。因此,愤怒应该更客观地衡量,而表达需要仔细检查。本研究的目的是为开发一种测量青少年愤怒表达类型的在线工具提供有效的验证。本研究收集了马来西亚北部9所学校935名青少年的数据,并进行了理论文献检索。对数据进行分析,从项目因子分析、信度估计和愤怒表达类型之间的相关性等方面提供结构效度的证据。研究结果被用于开发一个在线青少年愤怒工具。它测量了五种愤怒的表达方式,即身体愤怒、言语愤怒、内在愤怒、外在愤怒和被动愤怒。结果表明,该工具具有较高的构念效度。探索性因素分析,与变量旋转,表明存在五种不同类型的愤怒概念化。同时,愤怒表达类型之间的相关性表明了它们之间关系的强度。对研究结果进行了讨论,并对今后的研究提出了建议。
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引用次数: 0
Understanding International Students' Learning Experiences of English as a Medium of Instruction Courses 了解国际学生英语教学课程的学习经验
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.639
Ik-Soon Chung, Hyunyoung Choi
This study aims to understand international students' learning experiences in English as a medium of instruction (EMI) courses in a private Korean university. By using mixed-method research, this study collected two data sources, including 1) an online survey (n=46) and 2) an individual in-depth interview (n=4) at the end of the 2022 Spring semester. The study results are summarized as follows. First, EMI courses could enhance international students' content knowledge development effectively. Second, EMI courses could improve international students' English proficiency in terms of reading and vocabulary ability. Third, international students showed high satisfaction with EMI courses and are willing to take EMI courses continuously. However, the interview analysis showed that it was challenging for a few international students to improve their English speaking ability due to limited opportunities to speak English during graduate-level courses. These study results suggest several recommendations to provide practical EMI courses for teaching diverse international students, including English language learners in higher education.
本研究旨在了解韩国私立大学国际学生在英语教学中的学习经历。通过混合方法研究,本研究收集了两个数据来源,包括1)在线调查(n=46)和2)2022春季学期末的个人深度访谈(n=4)。研究结果总结如下。首先,EMI课程可以有效地促进国际学生的内容知识开发。其次,EMI课程可以提高国际学生的英语阅读和词汇能力。第三,国际学生对EMI课程表现出很高的满意度,并愿意继续学习EMI课程。然而,面试分析显示,由于在研究生级别的课程中讲英语的机会有限,少数国际学生提高英语口语能力具有挑战性。这些研究结果提出了一些建议,为不同的国际学生提供实用的EMI课程,包括高等教育中的英语学习者。
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引用次数: 1
Quality of Music Education in Croatian Music Schools: Students' Attitudes 克罗地亚音乐学校音乐教育质量:学生的态度
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.759
Vesna Svalina, Z. Šimunović
The purpose of this paper is to investigate how higher secondary music school students experience teaching, programs, and educational processes in music schools, which are the shortcomings and what are the suggestions for improving the system. The results show high satisfaction with attending music school related to the purpose of future professional orientation. Students see improvements in choosing more modern teaching topics, greater connection with practice, more time for student creativity, and work on improving teachers’ pedagogical skills. The results of this research could be used to accelerate the introduction of curriculum teaching and the National Curriculum for Art Education as a relevant document in music schools in the Republic of Croatia. Also, regardless of the inertia of the educational system, teachers can plan their teaching according to these results that will reconcile their ideas, methodological and didactic laws, and student expectations to contribute to greater motivation of their students.
本文的目的是调查高中音乐学校的学生如何体验音乐学校的教学、课程和教育过程,以及存在的不足和改进该系统的建议。研究结果表明,对进入音乐学校的未来职业定位目标有很高的满意度。学生们看到了在选择更现代的教学主题、与实践的更多联系、学生创造力的更多时间以及提高教师教学技能方面的进步。这项研究的结果可用于加快课程教学和《国家艺术教育课程》作为克罗地亚共和国音乐学校的相关文件的引入。此外,无论教育系统的惯性如何,教师都可以根据这些结果来规划教学,这些结果将调和他们的想法、方法和教学规律以及学生的期望,以提高学生的动力。
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引用次数: 1
Korean Pre-service Teachers' Flipped Learning Experiences in a Teacher Education Program 韩国职前教师在教师教育项目中的翻转学习体验
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.711
Yong-Jik Lee*, Seung-hoon Jeong, Yue Li
This study explores Korean pre-service teachers' perceptions, attitudes, and satisfaction with flipped learning courses in a teacher education program. A mixed research method was used to collect the end-of-semester survey (n=62) and individual interview data (n=4). Guided by the four pillars of flipped learning (FL), study results are followed. First, Korean pre-service teachers believed that flipped learning format provided a flexible learning environment where pre-service teachers (PSTs) learn course materials in their own space. This result indicated that their learning happened without the limits of time and space. Second, pre-service teachers showed that their self-directed learning could be enhanced while watching pre-recorded videos before attending the class. Most PSTs agreed that their self-directed learning could be enhanced after participating in the flipped learning courses. Third, pre-service teachers evaluated that the FL course instructor was a professional educator who conveyed the intentional content effectively. Fourth, pre-service teachers were generally satisfied with the FL courses and were willing to take the FL courses again in the future.
本研究探讨了韩国职前教师对教师教育项目中翻转学习课程的认知、态度和满意度。采用混合研究方法收集学期末调查(n=62)和个人访谈数据(n=4)。在翻转学习四大支柱的指导下,研究结果如下。首先,韩国职前教师认为,翻转学习模式提供了一个灵活的学习环境,职前教师(PSTs)可以在自己的空间学习课程材料。这一结果表明,他们的学习是不受时间和空间限制的。其次,职前教师表明,在上课前观看预先录制的视频可以增强他们的自主学习。大多数PST都认为,在参加翻转学习课程后,他们的自主学习可以得到加强。第三,职前教师评价外语课程教师是一位有效传达有意内容的专业教育者。第四,职前教师普遍对外语课程感到满意,并愿意在未来再次学习外语课程。
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引用次数: 1
Natural Disaster Education in School: A Bibliometric Analysis with a Detailed Future Insight Overview 学校自然灾害教育:文献计量学分析与详细的未来展望
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.743
A. Saregar, S. Sunyono, Een Yayah, H. Hariri, Fredi Ganda, R. Diani, M. Misbah, R. Umam
In some places of the world, disasters occur virtually every day. If disasters are not properly foreseen, they may result in many fatalities. This research aims to examine the growth and pattern of literature on natural disaster education in schools. This analytical strategy combines quantitative and statistical methods to discover trends, assess quality, and track development. A total of 216 documents were chosen from the 403 documents collected. Following 2015, there was a considerable increase in four-year publications. Authors from the United States contributed to 45 papers with 37% citations and ranked first, followed by authors from Japan (31 documents; 15% citations) and Indonesia (31 documents) with rankings citations below the top 10. Most publications were published in the International Journal of Disaster Risk Reduction (12 docs; Q1 Scimago Journal Rank 1.1 (SJR 1.1)). The Journal of Child Psychology and Psychiatry and Allied Disciplines had the most citations (100; Q1 SJR 3.6) and was ranked first in its discipline. The existing core literature on school education on natural disasters demonstrates that this topic is developing rapidly, but with insufficient international research collaboration. Research cooperation in this area must be strengthened to better the global response to natural disaster mitigation, which should begin in schools worldwide. There is a need to widen the scope of study in this field to include natural disaster preparedness education in the school curriculum, assessments, learning media, disaster response education, and instructional designs. Finally, disaster education in schools must be addressed as soon as possible to contribute to disaster preparedness.
在世界上的一些地方,灾难几乎每天都在发生。如果没有正确预见灾害,可能会导致许多人死亡。本研究旨在探讨学校自然灾害教育文献的成长与模式。这种分析策略结合了定量和统计方法来发现趋势、评估质量和跟踪发展。从收集的403份文件中,共挑选了216份文件。2015年之后,四年期出版物大幅增加。来自美国的作者贡献了45篇论文,引用率为37%,排名第一,其次是来自日本(31篇;引用率为15%)和印度尼西亚(31篇)的作者,引用率低于前10。大多数出版物发表在《国际减少灾害风险杂志》上(12篇文献;Q1 Scimago Journal Rank 1.1(SJR 1.1))。《儿童心理学与精神病学及相关学科杂志》被引用次数最多(100次;Q1 SJR 3.6),在其学科中排名第一。现有的关于自然灾害学校教育的核心文献表明,这一主题发展迅速,但国际研究合作不足。必须加强这一领域的研究合作,以更好地应对全球对自然灾害的缓解,这应该从世界各地的学校开始。有必要扩大这一领域的研究范围,将自然灾害防备教育纳入学校课程、评估、学习媒体、灾害应对教育和教学设计。最后,必须尽快解决学校的灾害教育问题,为备灾作出贡献。
{"title":"Natural Disaster Education in School: A Bibliometric Analysis with a Detailed Future Insight Overview","authors":"A. Saregar, S. Sunyono, Een Yayah, H. Hariri, Fredi Ganda, R. Diani, M. Misbah, R. Umam","doi":"10.12973/ijem.8.4.743","DOIUrl":"https://doi.org/10.12973/ijem.8.4.743","url":null,"abstract":"In some places of the world, disasters occur virtually every day. If disasters are not properly foreseen, they may result in many fatalities. This research aims to examine the growth and pattern of literature on natural disaster education in schools. This analytical strategy combines quantitative and statistical methods to discover trends, assess quality, and track development. A total of 216 documents were chosen from the 403 documents collected. Following 2015, there was a considerable increase in four-year publications. Authors from the United States contributed to 45 papers with 37% citations and ranked first, followed by authors from Japan (31 documents; 15% citations) and Indonesia (31 documents) with rankings citations below the top 10. Most publications were published in the International Journal of Disaster Risk Reduction (12 docs; Q1 Scimago Journal Rank 1.1 (SJR 1.1)). The Journal of Child Psychology and Psychiatry and Allied Disciplines had the most citations (100; Q1 SJR 3.6) and was ranked first in its discipline. The existing core literature on school education on natural disasters demonstrates that this topic is developing rapidly, but with insufficient international research collaboration. Research cooperation in this area must be strengthened to better the global response to natural disaster mitigation, which should begin in schools worldwide. There is a need to widen the scope of study in this field to include natural disaster preparedness education in the school curriculum, assessments, learning media, disaster response education, and instructional designs. Finally, disaster education in schools must be addressed as soon as possible to contribute to disaster preparedness.","PeriodicalId":92378,"journal":{"name":"International journal of educational methodology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42517575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Language Tests, Motivation, and Demotivation: A Complex Relationship 语言测试、动机和去动机:一种复杂的关系
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.699
Faris Keblawi
Tests are an integral part of most formal language learning processes, exerting far-reaching effects on many aspects of language learning. Among other things, tests affect language learning motivation (LLM), an element that is fundamental to many learning contexts. However, little attention has been paid to how tests affect LLM and what the pedagogical implications may be. Using qualitative semi-structured interviews with 20 adolescent Arab learners, this study attempts to explore how and when tests can motivate or demotivate language learners. It also examines the main strategies learners deploy to cope with the demotivating experiences associated with test taking. The data suggest that tests have the potential to both motivate and demotivate learners before, during, and after taking a test. Additionally, the results reveal several strategies that learners employ to cope with demotivation. This leads to a discussion regarding pedagogical implications on how to effectively employ tests to increase learners’ motivation and decrease their demotivation.   
考试是大多数正式语言学习过程的一个组成部分,对语言学习的许多方面产生深远的影响。除此之外,考试影响语言学习动机(LLM),这是许多学习环境的基本要素。然而,很少有人关注考试是如何影响法学硕士的,以及它的教学意义是什么。通过对20名阿拉伯青少年学习者的定性半结构化访谈,本研究试图探索测试如何以及何时可以激励或挫伤语言学习者。它还研究了学习者为应对与考试相关的消极经历而采用的主要策略。数据表明,考试在考试前、考试中和考试后都有可能激励和打击学习者。此外,研究结果还揭示了学习者用来应对动机丧失的几种策略。这导致了关于如何有效地利用测试来增加学习者的动机和减少他们的动机的教学意义的讨论。
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引用次数: 0
Students’ Challenges and Support Needed in Reaching Academic Literacies in Higher Education: In-Depth Interviews with Swedish Students 学生在高等教育中达到学术素养所面临的挑战和需要的支持——对瑞典学生的深度采访
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.731
Anna-Karin Svensson
The aim of this study is to capture and analyse perspectives on higher education from the views of five students through in-depth interviews. The students' statements are analyzed in order to gain an understanding of their experiences of developing academic literacies in their university studies. They are regularly visiting the university study workshop for help with their exam assignments. The following questions have been formulated to fulfil the aim of the study: (a) what aspects of studies in higher education do the students express as important, favorable, or unfavorable, for their development of academic literacies? and (b) in what way do the students value the study workshops as an educational tool? The analysis reveals three themes: the importance of explicit support structures, the importance of teachers’ feedback, and the importance of using the students’ pedagogical capital. The results also show that the students highly value the study workshop when they reflect upon the one-to-one feedback, but the support seems to be insufficient both in supporting them to be more independent in their studies and in developing academic literacies from a critical and epistemological point of view.
本研究的目的是通过深入访谈,从五名学生的角度捕捉和分析对高等教育的看法。对学生的陈述进行分析,以了解他们在大学学习中发展学术素养的经历。他们定期参观大学学习研讨会,寻求帮助完成考试作业。为了实现本研究的目的,我们提出了以下问题:(a)学生在高等教育中的哪些方面的学习对他们的学术素养发展是重要的、有利的还是不利的?以及(b)学生们以何种方式重视学习讲习班作为一种教育工具?分析揭示了三个主题:明确支持结构的重要性、教师反馈的重要性和利用学生教学资本的重要性。研究结果还表明,当学生反思一对一的反馈时,他们非常重视学习研讨会,但无论是在支持他们在学习中更加独立,还是从批判性和认识论的角度发展学术素养方面,这种支持似乎都不够。
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引用次数: 0
Number of Response Options, Reliability, Validity, and Potential Bias in the Use of the Likert Scale Education and Social Science Research: A Literature Review 教育与社会科学研究中李克特量表使用的回答选项数、信度、效度和潜在偏倚:文献综述
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.625
I. Kusmaryono, Dyana Wijayanti
This study reviews 60 papers using a Likert scale and published between 2012 – 2021. Screening for literature review uses the PRISMA method. The data analysis technique was carried out through data extraction, then synthesized in a structured manner using the narrative method. To achieve credible research results at the stage of the data collection and data analysis process, a group discussion forum (FGD) was conducted. The findings show that only 10% of studies use a measurement scale with an even answer choice category (4, 6, 8, or 10 choices). In general, (90%) of research uses a measurement instrument that involves a Likert scale with odd response choices (5, 7, 9, or 11) and the most popular researchers use a Likert scale with a total response of 5 points. The use of a rating scale with an odd number of responses of more than five points (especially on a seven-point scale) is the most effective in terms of reliability and validity coefficients, but if the researcher wants to direct respondents to one side, then a scale with an even number of responses (six points) is possible. more suitable. The presence of response bias and central tendency bias can affect the validity and reliability of the use of the Likert scale instrument.
本研究使用Likert量表回顾了2012年至2021年间发表的60篇论文。文献综述筛选采用PRISMA方法。数据分析技术是通过数据提取进行的,然后使用叙述方法以结构化的方式进行综合。为了在数据收集和数据分析过程阶段取得可信的研究结果,举办了一个小组讨论论坛。研究结果显示,只有10%的研究使用了偶数答案选择类别(4、6、8或10个选择)的测量量表。一般来说,(90%)的研究使用了一种测量仪器,该仪器涉及具有奇怪反应选择(5、7、9或11)的Likert量表,最受欢迎的研究人员使用的Liker特量表总反应为5分。就信度和有效性系数而言,使用奇数回答超过5分的评分量表(尤其是在7分的量表上)是最有效的,但如果研究人员想将受访者引向一边,那么偶数回答(6分)的量表是可能的。更合适。反应偏倚和中心倾向偏倚的存在会影响Likert量表使用的有效性和可靠性。
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引用次数: 4
The Implementation of Dual Language Programme for Mathematics Education in Secondary Schools: A Systematic Literature Review 中学数学双语教学计划的实施:系统的文献综述
Pub Date : 2022-11-15 DOI: 10.12973/ijem.8.4.669
N. Izzatie, R. Hidayat
The study discussed in this paper is a systematic literature review related to the role of dual language programme (DLP) in mathematics education which has been published within the last 5 years. This study was conducted to identify the distribution of DLP studies in terms of year of publication, the study context covered in previous studies, the context of study areas used, focus and trends of past studies, research methods used in previous studies and the role of language in school mathematics education. This review study followed the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) to analyze articles from Scopus and Web of Science. The findings of the study indicate that research trends in the implementation of the DLP on mathematics education for secondary school students showed an increase from 2017 to 2019. Most DLP-related articles are widely developed in the United States and Germany. The findings indicate that previous studies are more interested in studying the implementation of DLP in rural areas. Past studies have also preferred to use the design of either a qualitative study or a quantitative study to be implemented. Questionnaires, tests and interviews are among the research instruments that are often used for a study.
本文讨论的研究是对近5年来发表的有关双语课程(DLP)在数学教育中的作用的文献进行系统的综述。本研究旨在确定DLP研究在出版年份、以往研究涵盖的研究背景、使用的研究领域背景、过去研究的重点和趋势、以往研究使用的研究方法以及语言在学校数学教育中的作用等方面的分布。本综述研究遵循系统评价和元分析首选报告项目(PRISMA)的指导方针,分析来自Scopus和Web of Science的文章。研究结果表明,2017年至2019年,实施DLP对中学生数学教育的研究趋势呈上升趋势。大多数与dlp相关的文章是在美国和德国广泛开发的。研究结果表明,以往的研究更倾向于研究DLP在农村地区的实施情况。过去的研究也倾向于使用定性研究或定量研究的设计来实施。问卷调查、测试和访谈是研究中经常使用的研究工具。
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引用次数: 3
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International journal of educational methodology
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