Abstract Objectives The search for genetic mutations is very important in younger patients and other age groups with a history of recurrent cerebrovascular diseases (CVD) and a family history of other causes to be excluded. The aim of this study is to define the characteristics of genetic mutations in the etiology of ischemic stroke. Methods Twenty-three patients with acute CVD in the last 1 year and only genetic mutations acknowledged in the etiology were retrospectively analyzed. We determined the frequency of the genetic mutations that are observed in cerebral arterial events (CAE) and cerebral venous thrombosis (CVT). Results All patients had at least one genetic mutation and 19 of them had arterial events and 4 had venous thrombosis. MTHFR mutation was the most common mutation and PAI-1 mutation was the second in line for the arterial events. PAI 4G/5G, MTHFR A1298 and FV mutations were most frequently observed in venous events. Patent foramen ovale (PFO) was detected in 14 patients (%74) with CAE. Conclusions We concluded that multiple gene mutations may significantly increase the development of CVD. CVD is most commonly associated with MTHFR, PAI-1 or FV gene mutations and is most commonly seen in CAE. MTHFR mutations showed moderate linear correlation in the development of arterial events and FXII and FXIII mutations in venous events. The association of thrombophilia and PFO is high in patients who have undergone CAE, especially responsible for recurrent events. This study will need to be confirmed by prospective studies with larger sample and control group.
{"title":"Ischemic cerebrovascular disease caused by genetic mutation and patent foramen ovale","authors":"Esra Eruyar, Tevfik Honca, Fatih Bakır","doi":"10.1515/tjb-2023-0187","DOIUrl":"https://doi.org/10.1515/tjb-2023-0187","url":null,"abstract":"Abstract Objectives The search for genetic mutations is very important in younger patients and other age groups with a history of recurrent cerebrovascular diseases (CVD) and a family history of other causes to be excluded. The aim of this study is to define the characteristics of genetic mutations in the etiology of ischemic stroke. Methods Twenty-three patients with acute CVD in the last 1 year and only genetic mutations acknowledged in the etiology were retrospectively analyzed. We determined the frequency of the genetic mutations that are observed in cerebral arterial events (CAE) and cerebral venous thrombosis (CVT). Results All patients had at least one genetic mutation and 19 of them had arterial events and 4 had venous thrombosis. MTHFR mutation was the most common mutation and PAI-1 mutation was the second in line for the arterial events. PAI 4G/5G, MTHFR A1298 and FV mutations were most frequently observed in venous events. Patent foramen ovale (PFO) was detected in 14 patients (%74) with CAE. Conclusions We concluded that multiple gene mutations may significantly increase the development of CVD. CVD is most commonly associated with MTHFR, PAI-1 or FV gene mutations and is most commonly seen in CAE. MTHFR mutations showed moderate linear correlation in the development of arterial events and FXII and FXIII mutations in venous events. The association of thrombophilia and PFO is high in patients who have undergone CAE, especially responsible for recurrent events. This study will need to be confirmed by prospective studies with larger sample and control group.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"199 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134977975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bülent Gözcelioğlu, İbrahim Seyda Uras, Murat Şentürk, Belma Konuklugil
Abstract Objectives Marine organisms obtained from Antarctica are prominent sources for many important activities. Algae are known for adapting to various adverse environmental conditions and for producing secondary metabolites with various biological activities. This study examined the enzyme inhibitory properties of six different Antarctic algal extracts. Methods We investigated the activity of specific enzymes, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA I/II), glutathione reductase (GR), and α-glucosidase (AG), as these enzymes have potential therapeutic applications such as in Alzheimer’s disease, malaria, cancer, and diabetes mellitus. Results The results of the study found that the algal extracts had potent inhibitory effects on these enzymes, with IC 50 values ranging from 0.60 to 48.85 μg/mL, indicating that these extracts could be source of potential new drugs. Monostroma harioti and Cystosphaera jacquinotii extracts demonstrated highest AChE and CA I enzymes inhibiton. M. harioti and Desmarestia antarctica extracts presented highest GR enzyme inhibiton, C. jacquinotii and D. antarctica extracts presented highest inhibitory activity against BChE, CA II and α-glucosidase enzymes. Conclusions Extracts of algae samples taken from Antarctica have high enzyme inhibitory activity, and further studies are needed to find out which compounds may be responsible for the effect.
{"title":"Exploring the enzyme inhibitory properties of Antarctic algal extracts","authors":"Bülent Gözcelioğlu, İbrahim Seyda Uras, Murat Şentürk, Belma Konuklugil","doi":"10.1515/tjb-2023-0103","DOIUrl":"https://doi.org/10.1515/tjb-2023-0103","url":null,"abstract":"Abstract Objectives Marine organisms obtained from Antarctica are prominent sources for many important activities. Algae are known for adapting to various adverse environmental conditions and for producing secondary metabolites with various biological activities. This study examined the enzyme inhibitory properties of six different Antarctic algal extracts. Methods We investigated the activity of specific enzymes, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA I/II), glutathione reductase (GR), and α-glucosidase (AG), as these enzymes have potential therapeutic applications such as in Alzheimer’s disease, malaria, cancer, and diabetes mellitus. Results The results of the study found that the algal extracts had potent inhibitory effects on these enzymes, with IC 50 values ranging from 0.60 to 48.85 μg/mL, indicating that these extracts could be source of potential new drugs. Monostroma harioti and Cystosphaera jacquinotii extracts demonstrated highest AChE and CA I enzymes inhibiton. M. harioti and Desmarestia antarctica extracts presented highest GR enzyme inhibiton, C. jacquinotii and D. antarctica extracts presented highest inhibitory activity against BChE, CA II and α-glucosidase enzymes. Conclusions Extracts of algae samples taken from Antarctica have high enzyme inhibitory activity, and further studies are needed to find out which compounds may be responsible for the effect.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.1515/tjb-2023-frontmatter5
{"title":"Frontmatter","authors":"","doi":"10.1515/tjb-2023-frontmatter5","DOIUrl":"https://doi.org/10.1515/tjb-2023-frontmatter5","url":null,"abstract":"","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136010218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives This study aims to comprehensively evaluate extracellular vesicle (EV)-based biomarkers circulating in body fluids with significant prognostic value in breast cancer (BrCa). Methods We systematically searched WOS, PubMed, and Scopus databases on 14 February 2023 for studies indicating overall survival(OS), progression/disease/event-free survival(PFS/DFS/EFS), and metastatic progression. We computed univariate(UHR) or multivariate adjusted(AHR) hazard ratios, and AUC values for all prognostic EV-based biomarkers of blood-origin using random effect model and Stata 16.0 software. Subgroup analysis was conducted for positive and negative prognostic factors. Results Twenty-one articles comprising twenty-six studies and 3,423 patients satisfied the inclusion criteria. EV-based negative biomarkers indicated low OS(UHR=2.31, CI=1.77–3.03, I 2 =60.12 %, p<0.001); worse DFS/PFS/EFS(UHR=3.91, CI=2.82–5.43, I 2 =19.08 %, p=0.24); increased risk for metastasis(pooled AUC=0.91). Out of 56 EV-based biomarkers that have been previously described, we identified PD-L2, sHLA-G, exo-XIST, and miR4800 as the best predictors of OS of BrCa patients. Expression levels of miR155, Annexin-A2, sHLA-G, PD-L2, miR1246, PSMA and the biomarkers constructing the EV P -panel hold significant potential to be combined in a prognostic-panel predicting DFS/PFS/EFS of BrCa patients. PD-L2 and sHLA-G standing out as leading biomarkers in both OS and DFS highlights the importance of immune system evasion for patient survival. In addition, we suggest that reinforcement with additional RNA biomarkers could significantly increase the metastatic prediction power of the previously described EV DX -panel. Conclusions This meta-analysis provides an overview of the liquid biopsy-based EV-biomarkers associated with OS, DFS, and metastatic progression of BrCa for the first time. Prognostic efficiency of the proposed panels should be further investigated before transition to clinical use.
{"title":"Exosomal prognostic biomarkers predict metastatic progression and survival in breast cancer patients","authors":"Ceyhan Ceran Serdar, Şeyma Osmanlıoğlu","doi":"10.1515/tjb-2023-0142","DOIUrl":"https://doi.org/10.1515/tjb-2023-0142","url":null,"abstract":"Abstract Objectives This study aims to comprehensively evaluate extracellular vesicle (EV)-based biomarkers circulating in body fluids with significant prognostic value in breast cancer (BrCa). Methods We systematically searched WOS, PubMed, and Scopus databases on 14 February 2023 for studies indicating overall survival(OS), progression/disease/event-free survival(PFS/DFS/EFS), and metastatic progression. We computed univariate(UHR) or multivariate adjusted(AHR) hazard ratios, and AUC values for all prognostic EV-based biomarkers of blood-origin using random effect model and Stata 16.0 software. Subgroup analysis was conducted for positive and negative prognostic factors. Results Twenty-one articles comprising twenty-six studies and 3,423 patients satisfied the inclusion criteria. EV-based negative biomarkers indicated low OS(UHR=2.31, CI=1.77–3.03, I 2 =60.12 %, p<0.001); worse DFS/PFS/EFS(UHR=3.91, CI=2.82–5.43, I 2 =19.08 %, p=0.24); increased risk for metastasis(pooled AUC=0.91). Out of 56 EV-based biomarkers that have been previously described, we identified PD-L2, sHLA-G, exo-XIST, and miR4800 as the best predictors of OS of BrCa patients. Expression levels of miR155, Annexin-A2, sHLA-G, PD-L2, miR1246, PSMA and the biomarkers constructing the EV P -panel hold significant potential to be combined in a prognostic-panel predicting DFS/PFS/EFS of BrCa patients. PD-L2 and sHLA-G standing out as leading biomarkers in both OS and DFS highlights the importance of immune system evasion for patient survival. In addition, we suggest that reinforcement with additional RNA biomarkers could significantly increase the metastatic prediction power of the previously described EV DX -panel. Conclusions This meta-analysis provides an overview of the liquid biopsy-based EV-biomarkers associated with OS, DFS, and metastatic progression of BrCa for the first time. Prognostic efficiency of the proposed panels should be further investigated before transition to clinical use.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135407267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Chronic kidney disease (CKD) is a condition characterized by atherosclerosis, cognitive impairment, physical limitations, biochemical abnormalities, and vascular aging. The proportion of those with a diagnosis of CKD in the older is increasing. With comprehensive geriatric assessment, it could be possible to detect the disorders that are related to biological aging. The aim is to evaluate geriatric syndromes like frailty, cognitive dysfunction, malnutrition, and polypharmacy in an aged population with pre-dialytic CKD (stages 3a–5), and to investigate possible relations with biochemical features and anticholinergic drug burden (ADB). Methods One hundred and fifty-six CKD patients aged 60 and older and 164 healthy controls were included in the study. Geriatric parameters that were used for the evaluation of the groups were, Clinical Frailty Index; Charlson Comorbidity Index; Montreal Cognitive Assessment and Mini Nutritional Assessment Short-Form. Besides, biochemical parameters and ADB defined with 3 scales Anticholinergic Burden Classification (ABC), Chew’s scale, and Drug Burden Index were recorded. Results Despite being younger, CKD patients had higher comorbidity and frailty scores than the controls. Patients and controls had similar nutritional status, and cognitive function test results. Frailty was an important predictor for geriatric parameters and eGFR. ABC score was higher in the CKD group in ADB scale. Conclusions Frailty and polypharmacy are more prevalent than expected in older with CKD. In addition, anticholinergic burden and polypharmacy may form causal links with one and other and lead to increased mortality rates especially with frailty. Therefore, geriatric assessment and appropriate ADB evaluation may be recommended in CKD patients.
{"title":"Comprehensive geriatric assessment and drug burden in elderly chronic kidney disease patients","authors":"Neziha Erken, Ertugrul Erken","doi":"10.1515/tjb-2023-0189","DOIUrl":"https://doi.org/10.1515/tjb-2023-0189","url":null,"abstract":"Abstract Objectives Chronic kidney disease (CKD) is a condition characterized by atherosclerosis, cognitive impairment, physical limitations, biochemical abnormalities, and vascular aging. The proportion of those with a diagnosis of CKD in the older is increasing. With comprehensive geriatric assessment, it could be possible to detect the disorders that are related to biological aging. The aim is to evaluate geriatric syndromes like frailty, cognitive dysfunction, malnutrition, and polypharmacy in an aged population with pre-dialytic CKD (stages 3a–5), and to investigate possible relations with biochemical features and anticholinergic drug burden (ADB). Methods One hundred and fifty-six CKD patients aged 60 and older and 164 healthy controls were included in the study. Geriatric parameters that were used for the evaluation of the groups were, Clinical Frailty Index; Charlson Comorbidity Index; Montreal Cognitive Assessment and Mini Nutritional Assessment Short-Form. Besides, biochemical parameters and ADB defined with 3 scales Anticholinergic Burden Classification (ABC), Chew’s scale, and Drug Burden Index were recorded. Results Despite being younger, CKD patients had higher comorbidity and frailty scores than the controls. Patients and controls had similar nutritional status, and cognitive function test results. Frailty was an important predictor for geriatric parameters and eGFR. ABC score was higher in the CKD group in ADB scale. Conclusions Frailty and polypharmacy are more prevalent than expected in older with CKD. In addition, anticholinergic burden and polypharmacy may form causal links with one and other and lead to increased mortality rates especially with frailty. Therefore, geriatric assessment and appropriate ADB evaluation may be recommended in CKD patients.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135768499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Type 1 diabetes mellitus is a metabolic disease characterized by dysbiosis. Modulation of the gut microbiota by oligosaccharides and aerobic training are proposed mechanisms that ameliorate the disease through affecting host-microbiota interactions. Methods Seventy-two male Wistar rats were randomly divided into 8 groups – 5 with streptozotocin-induced diabetes and 3 healthy controls. The effect of two oligosaccharides – xylo- and galactooligosaccharides, and of aerobic training on the blood glucose concentration, growth and diversity of the gut microbiota was evaluated in the current study. Results The galactooligosaccharides positively affected the glycemic status of the experimental animals as the diabetic and healthy rats had lower blood glucose concentration after 6 weeks of treatment (diabetic rats: week 4 vs. week 8, p=0.047; healthy rats: week 2,4,6,10 vs. week 8, p=0.001, p=0.000, p=0.025 and p=0.001, respectively). A positive effect of the galactooligosaccharides on body weight was observed when administered to diabetic rats in comparison to the diabetic control (p=0.020). Similar results were observed for the aerobically trained diabetic rats (p=0.004). The identification of bacterial species showed preserved microbiota diversity and indicated Bifidobacterium indicum , Lactobacillus feritoshensis and E. coli as the most abundant species among the analyzed genera. Conclusions Prebiotic treatment beneficially affected the hyperglycemia and growth of type 1 diabetic rats. The most significant effect of the aerobic training was the improvement of the morphological parameters. Oligosaccharide administration and exercise did not affect the diversity of the bacterial species.
{"title":"Effect of oligosaccharides and aerobic training on hyperglycemia, growth and intestinal microbial diversity of diabetic rats","authors":"Mariya Atanasova Choneva, Milen Veselinov Hristozov, Ivica Dimov, Krasimir Ognyanov Boyanov, Iliyan Valeriev Dimitrov, Mariana Atanasova Murdjeva, Petar Ivanov Hrischev, Veselin Atanasov Vasilev, Katerina Nikolova Georgieva, Anelia Veselinova Bivolarska","doi":"10.1515/tjb-2023-0070","DOIUrl":"https://doi.org/10.1515/tjb-2023-0070","url":null,"abstract":"Abstract Objectives Type 1 diabetes mellitus is a metabolic disease characterized by dysbiosis. Modulation of the gut microbiota by oligosaccharides and aerobic training are proposed mechanisms that ameliorate the disease through affecting host-microbiota interactions. Methods Seventy-two male Wistar rats were randomly divided into 8 groups – 5 with streptozotocin-induced diabetes and 3 healthy controls. The effect of two oligosaccharides – xylo- and galactooligosaccharides, and of aerobic training on the blood glucose concentration, growth and diversity of the gut microbiota was evaluated in the current study. Results The galactooligosaccharides positively affected the glycemic status of the experimental animals as the diabetic and healthy rats had lower blood glucose concentration after 6 weeks of treatment (diabetic rats: week 4 vs. week 8, p=0.047; healthy rats: week 2,4,6,10 vs. week 8, p=0.001, p=0.000, p=0.025 and p=0.001, respectively). A positive effect of the galactooligosaccharides on body weight was observed when administered to diabetic rats in comparison to the diabetic control (p=0.020). Similar results were observed for the aerobically trained diabetic rats (p=0.004). The identification of bacterial species showed preserved microbiota diversity and indicated Bifidobacterium indicum , Lactobacillus feritoshensis and E. coli as the most abundant species among the analyzed genera. Conclusions Prebiotic treatment beneficially affected the hyperglycemia and growth of type 1 diabetic rats. The most significant effect of the aerobic training was the improvement of the morphological parameters. Oligosaccharide administration and exercise did not affect the diversity of the bacterial species.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135770462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ömer Faruk Çiçek, Fikret Akyürek, Hakan Akbayrak, Atilla Orhan, Eyüp Cihan Kaya, Mustafa Büyükateş
Abstract Objectives The aim of this study was to investigate the potential of preoperative neopterin levels as a predictive marker for postoperative acute kidney injury (AKI) in patients undergoing on-pump cardiac surgery, in addition to other potential risk factors. Methods This observational study included 91 patients who underwent elective cardiac surgery under cardiopulmonary bypass. Of these, 35 patients (38.46 %) experienced AKI following surgery, as outlined by the Kidney Disease Improving Global Outcomes (KDIGO) standards. The study participants were divided into two groups depending on whether they had developed AKI after the surgery or not. The study compared two groups and utilized logistic regression analysis to evaluate potential predictors. A receiver operating characteristic (ROC) analysis was conducted to determine the ability of preoperative neopterin levels to predict the occurrence of AKI. Results A comparison of the baseline demographic, clinical, laboratory, and echocardiographic characteristics was conducted between patients who suffered from AKI and those who did not. The multivariate analysis demonstrated that EuroSCORE II (OR, 4.525; 95 % CI, 1.29–15.87; p=0.019), X-clamp time (OR, 1.157; 95 % CI, 1.01–1.326; p=0.035), and neopterin levels (OR, 22.952; 95 % CI, 3.14–167.763; p=0.002) were independently predicted the post-cardiac surgery AKI. ROC analysis identified a cut-off value of 9.65 nmol/L, which had a sensitivity of 91.4 % and a specificity of 91.1 % (area under the curve, 0.98; 95 % CI, 0.958–1; p<0.001). Conclusions Our study emphasizes the potential of preoperative neopterin levels, EuroSCORE II, and X-clamp time as independent predictors of postoperative AKI, even in milder cases, in individuals undergoing on-pump cardiac surgery.
{"title":"Can preoperative neopterin levels predict acute kidney injury in patients undergoing on-pump cardiac surgery?","authors":"Ömer Faruk Çiçek, Fikret Akyürek, Hakan Akbayrak, Atilla Orhan, Eyüp Cihan Kaya, Mustafa Büyükateş","doi":"10.1515/tjb-2023-0074","DOIUrl":"https://doi.org/10.1515/tjb-2023-0074","url":null,"abstract":"Abstract Objectives The aim of this study was to investigate the potential of preoperative neopterin levels as a predictive marker for postoperative acute kidney injury (AKI) in patients undergoing on-pump cardiac surgery, in addition to other potential risk factors. Methods This observational study included 91 patients who underwent elective cardiac surgery under cardiopulmonary bypass. Of these, 35 patients (38.46 %) experienced AKI following surgery, as outlined by the Kidney Disease Improving Global Outcomes (KDIGO) standards. The study participants were divided into two groups depending on whether they had developed AKI after the surgery or not. The study compared two groups and utilized logistic regression analysis to evaluate potential predictors. A receiver operating characteristic (ROC) analysis was conducted to determine the ability of preoperative neopterin levels to predict the occurrence of AKI. Results A comparison of the baseline demographic, clinical, laboratory, and echocardiographic characteristics was conducted between patients who suffered from AKI and those who did not. The multivariate analysis demonstrated that EuroSCORE II (OR, 4.525; 95 % CI, 1.29–15.87; p=0.019), X-clamp time (OR, 1.157; 95 % CI, 1.01–1.326; p=0.035), and neopterin levels (OR, 22.952; 95 % CI, 3.14–167.763; p=0.002) were independently predicted the post-cardiac surgery AKI. ROC analysis identified a cut-off value of 9.65 nmol/L, which had a sensitivity of 91.4 % and a specificity of 91.1 % (area under the curve, 0.98; 95 % CI, 0.958–1; p<0.001). Conclusions Our study emphasizes the potential of preoperative neopterin levels, EuroSCORE II, and X-clamp time as independent predictors of postoperative AKI, even in milder cases, in individuals undergoing on-pump cardiac surgery.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136235942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Although MOTS-C has been reported to have a role in diabetes mellitus, no human studies have evaluated the serum level of MOTS-C in GDM. It was aimed to investigate serum levels of MOTS-C in patients with gestational diabetes mellitus (GDM). Methods Comparisons were made of 44 pregnant patients diagnosed with GDM and a control group of 44 healthy pregnant women in respect of serum MOTS-C, insulin, and glucose levels, and serum lipid profile. Results A significantly higher level of fasting serum glucose and significantly lower serum levels of MOTS-C and high density lipoprotein were determined in the GDM group compared to the control group (p<0.05 for all). A cut-off value of 173.5 ng/mL for serum MOTS-C level had sensitivity of 81.8 % and specificity of 61.4 % for GDM diagnosis (p<0.001). A significant correlation was determined between the serum MOTS-C and serum glucose levels (r=−0.239, p=0.025). Conclusions For the first time in literature, the results of this study showed that patients with GDM had a decreased serum level of MOTS-C and that increasing serum MOTS-C levels were associated with a decrease in serum glucose levels, thereby supporting the view that mitochondrial dysfunction plays a role in GDM pathogenesis. Therefore, MOTS-C could be a promising diagnostic biomarker for GDM cases.
{"title":"Gestational diabetes mellitus is associated with a low serum level of mitochondrial-derived peptide-MOTS-C","authors":"Serdar Özer, Filiz Alkan Baylan, Abdullah Tok","doi":"10.1515/tjb-2022-0097","DOIUrl":"https://doi.org/10.1515/tjb-2022-0097","url":null,"abstract":"Abstract Objectives Although MOTS-C has been reported to have a role in diabetes mellitus, no human studies have evaluated the serum level of MOTS-C in GDM. It was aimed to investigate serum levels of MOTS-C in patients with gestational diabetes mellitus (GDM). Methods Comparisons were made of 44 pregnant patients diagnosed with GDM and a control group of 44 healthy pregnant women in respect of serum MOTS-C, insulin, and glucose levels, and serum lipid profile. Results A significantly higher level of fasting serum glucose and significantly lower serum levels of MOTS-C and high density lipoprotein were determined in the GDM group compared to the control group (p<0.05 for all). A cut-off value of 173.5 ng/mL for serum MOTS-C level had sensitivity of 81.8 % and specificity of 61.4 % for GDM diagnosis (p<0.001). A significant correlation was determined between the serum MOTS-C and serum glucose levels (r=−0.239, p=0.025). Conclusions For the first time in literature, the results of this study showed that patients with GDM had a decreased serum level of MOTS-C and that increasing serum MOTS-C levels were associated with a decrease in serum glucose levels, thereby supporting the view that mitochondrial dysfunction plays a role in GDM pathogenesis. Therefore, MOTS-C could be a promising diagnostic biomarker for GDM cases.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136236356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives To determine the ideal timing of labor for term pregnancies using oxidative stress determiners. Materials and methods 150 low-risk pregnant women were divided into three groups according to gestational week periods: early term, full term, and late-term. Groups were matched in terms of age and body mass index. Thiol/disulfide homeostasis parameters were obtained from maternal blood samples at the beginning of the active phase and from cord blood immediately after delivery. Results Maternal and fetal native and total thiol, which indicates antioxidant status, were found to be significantly higher in full terms (maternal 397.5 ± 78.8, 435.9 ± 84.5, and fetal 472.5 ± 78.4, 513.7 ± 89.2 respectively; for all parameters p<0.05). The maternal oxidant balance was not significantly different between the groups; 1st-minute APGAR scores were significantly correlated with maternal antioxidant levels (p=0.042; r=0.199). Conclusions The highest level of antioxidants in thiol/disulfide homeostasis in full-term pregnancies can be explained by the mechanism that increases the antioxidant level of the mother, which works best in the period between 39 and 41 weeks of pregnancy.
{"title":"Ideal timing of labor in terms of oxidative stress – which term period is best?","authors":"Gamze Yilmaz, Salim Neselioglu, Fatma Nur Ceylan, Burak Elmas, Fatmanur Ece Aydogdu, Ozcan Erel, Ozlem Uzunlar, Esma Sarikaya, Ozlem Moraloglu Tekin","doi":"10.1515/tjb-2023-0098","DOIUrl":"https://doi.org/10.1515/tjb-2023-0098","url":null,"abstract":"Abstract Objectives To determine the ideal timing of labor for term pregnancies using oxidative stress determiners. Materials and methods 150 low-risk pregnant women were divided into three groups according to gestational week periods: early term, full term, and late-term. Groups were matched in terms of age and body mass index. Thiol/disulfide homeostasis parameters were obtained from maternal blood samples at the beginning of the active phase and from cord blood immediately after delivery. Results Maternal and fetal native and total thiol, which indicates antioxidant status, were found to be significantly higher in full terms (maternal 397.5 ± 78.8, 435.9 ± 84.5, and fetal 472.5 ± 78.4, 513.7 ± 89.2 respectively; for all parameters p<0.05). The maternal oxidant balance was not significantly different between the groups; 1st-minute APGAR scores were significantly correlated with maternal antioxidant levels (p=0.042; r=0.199). Conclusions The highest level of antioxidants in thiol/disulfide homeostasis in full-term pregnancies can be explained by the mechanism that increases the antioxidant level of the mother, which works best in the period between 39 and 41 weeks of pregnancy.","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135825085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soner Yesilyurt, Osman Erinc, Almila Senat, Cem Tugrul Gezmis, Mustafa Bahadir Can Balci
Abstract Objectives In this study, we aimed to evaluate the relationship between ABO blood groups and Rhesus factor (Rf) and severe acute respiratory syndrome coronavirus-2 (Sars-CoV-2), as well as the risk of infection susceptibility and death according to pre-existing comorbidities. Methods This retrospective study included patients medical record between March 2020 and March 2021. A total 470 patients were included in the study. The subjects were categorized according to diagnose of Sars-CoV-2. Also, we evaluated the subject according to severity of Sars-CoV-2 infection. The logistic and multivariate regression analysis were performed to predict possible effect of ABO and Rf types as well as comorbidities on indicators of Sars-CoV-2 severity including Intensive care unit (ICU) hospitalization, intubation, and mortality. Results The distribution of ABO blood type and Rf were not statistically different cases with and without Sars-CoV-2. Blood type B and A were the most groups in intubation and mortality among patients with Sars-CoV-2. However, ABO blood types had no significant effect on risk of Sars-CoV-2 and mortality while, Rf had a significantly effect on it. Additionally, Rf had a statistically significant effect on all severity indicators of Sars-CoV-2 but ABO had not. Conclusions While Rf was significantly associated with risk of Sars-CoV-2 and had a strong effect on ICU admission, intubation, and mortality, ABO groups were not associated with risk of disease. Intubation and mortality rates were higher in patients with blood group B (OR: 2.93 p:0.390 95 % CI [0.253–33.9], OR: 0.217 p:0.211 95 % CI [0.020–2.37]) and Rh factor + (OR: 1.63 p:0.027 95 % CI [0.046–0.828]).
{"title":"Rhesus factor is a stronger predictor for the risk of Sars-CoV-2 and mortality than ABO blood types","authors":"Soner Yesilyurt, Osman Erinc, Almila Senat, Cem Tugrul Gezmis, Mustafa Bahadir Can Balci","doi":"10.1515/tjb-2023-0063","DOIUrl":"https://doi.org/10.1515/tjb-2023-0063","url":null,"abstract":"Abstract Objectives In this study, we aimed to evaluate the relationship between ABO blood groups and Rhesus factor (Rf) and severe acute respiratory syndrome coronavirus-2 (Sars-CoV-2), as well as the risk of infection susceptibility and death according to pre-existing comorbidities. Methods This retrospective study included patients medical record between March 2020 and March 2021. A total 470 patients were included in the study. The subjects were categorized according to diagnose of Sars-CoV-2. Also, we evaluated the subject according to severity of Sars-CoV-2 infection. The logistic and multivariate regression analysis were performed to predict possible effect of ABO and Rf types as well as comorbidities on indicators of Sars-CoV-2 severity including Intensive care unit (ICU) hospitalization, intubation, and mortality. Results The distribution of ABO blood type and Rf were not statistically different cases with and without Sars-CoV-2. Blood type B and A were the most groups in intubation and mortality among patients with Sars-CoV-2. However, ABO blood types had no significant effect on risk of Sars-CoV-2 and mortality while, Rf had a significantly effect on it. Additionally, Rf had a statistically significant effect on all severity indicators of Sars-CoV-2 but ABO had not. Conclusions While Rf was significantly associated with risk of Sars-CoV-2 and had a strong effect on ICU admission, intubation, and mortality, ABO groups were not associated with risk of disease. Intubation and mortality rates were higher in patients with blood group B (OR: 2.93 p:0.390 95 % CI [0.253–33.9], OR: 0.217 p:0.211 95 % CI [0.020–2.37]) and Rh factor + (OR: 1.63 p:0.027 95 % CI [0.046–0.828]).","PeriodicalId":92463,"journal":{"name":"Turk biyokimya dergisi = Turkish journal of biochemistry","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135830976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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