M. Nosova, II Latif, L. Kraeva, G. Khamdulaeva, A. Sharov, I. Kopetskiy, Daniil N. Eremin, E. Postnikova, M. Postnikov
Periodontitis is a problem urgent in Russia and throughout the world in general. Because of the dynamically changing flora causing this diseases, the treatment methods designed against it should be adapted on a regular basis. The classic approach to arresting development of the acute process relies on 0.2–0.12% chlorhexidine, a chemical antiseptic, but after 3 weeks of use, its efficacy drops drastically because pathogenic flora adjusts thereto. In the recent years, plantbased complexes with antiseptic properties have shown their capacity to challenge the classic approach. Obviously, efficacy of active ingredients depends on the form of the final product. The marker of periodontitis in the oral cavity is Staphylococcus aureus. Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii have virulence markers that are copathogens for periodontitis. This study aimed to find plant-based preparations capable of eliminating the said microbes and Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Enterococcus faecalis. We compared antibacterial, adhesion and biofilm formation preventing properties of Phytodent plant-based products in various forms: water solution, water-alcohol solution, oil solution, gel. Long exposure form — gel — proved to be the most effective in terms of the properties tested. Products with synthetic and plant-based antiseptics, as well as those with plant-based antiseptics in maximum concentration (elixir), had comparable efficacy. Water and oil solutions are less effective because of the lower active ingredient concentration and relatively brief exposure. Our results support the results of clinical studies dedicated to the use of Phytodent products as oral care products in the context of periodontitis prevention and treatment. We recommend conducting further studies comparing compositions, cross- and comparative studies investigating the effect of frequency of application and time of exposure, such studies registering titers of active ingredient concentrations, and with subjects thereof including mixed biofilms.
{"title":"Antibacterial, anti-adhesive and anti-biofilm-forming activity of plant complexes against periodontopathogenic bacteria in vitro","authors":"M. Nosova, II Latif, L. Kraeva, G. Khamdulaeva, A. Sharov, I. Kopetskiy, Daniil N. Eremin, E. Postnikova, M. Postnikov","doi":"10.24075/brsmu.2023.029","DOIUrl":"https://doi.org/10.24075/brsmu.2023.029","url":null,"abstract":"Periodontitis is a problem urgent in Russia and throughout the world in general. Because of the dynamically changing flora causing this diseases, the treatment methods designed against it should be adapted on a regular basis. The classic approach to arresting development of the acute process relies on 0.2–0.12% chlorhexidine, a chemical antiseptic, but after 3 weeks of use, its efficacy drops drastically because pathogenic flora adjusts thereto. In the recent years, plantbased complexes with antiseptic properties have shown their capacity to challenge the classic approach. Obviously, efficacy of active ingredients depends on the form of the final product. The marker of periodontitis in the oral cavity is Staphylococcus aureus. Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii have virulence markers that are copathogens for periodontitis. This study aimed to find plant-based preparations capable of eliminating the said microbes and Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Enterococcus faecalis. We compared antibacterial, adhesion and biofilm formation preventing properties of Phytodent plant-based products in various forms: water solution, water-alcohol solution, oil solution, gel. Long exposure form — gel — proved to be the most effective in terms of the properties tested. Products with synthetic and plant-based antiseptics, as well as those with plant-based antiseptics in maximum concentration (elixir), had comparable efficacy. Water and oil solutions are less effective because of the lower active ingredient concentration and relatively brief exposure. Our results support the results of clinical studies dedicated to the use of Phytodent products as oral care products in the context of periodontitis prevention and treatment. We recommend conducting further studies comparing compositions, cross- and comparative studies investigating the effect of frequency of application and time of exposure, such studies registering titers of active ingredient concentrations, and with subjects thereof including mixed biofilms.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44589298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VB Pavlenko, S. Vlasenko, LS Orekhova, EA Biryukova
As explained earlier, neurorehabilitation sessions involving the use of the non-invasive “brain – computer – hand exoskeleton” interface reduce hand muscle spasticity and improve motor skills in children with cerebral palsy (CP). However, the changes in the patients’ speech functions and their relationship with the upper limb mobility have not been analyzed. The study was aimed to assess the correlation between the motor and speech functions of children with CP, as well as to detect the changes in motor realization of speech production following complex treatment of patients including sessions of neurorehabilitation. The study involved children with CP aged 6–15. The index group (n = 40, 16 girls, 24 boys) received complex resort treatment with the course of neurorehabilitation, while the comparison group (n = 20, 10 girls, 10 boys) received standard resort treatment. A significant (р < 0.001) correlation between the total ABILHAND-Kids score and the indicators of speech production motor realization was revealed. In patients of the index group, complex treatment with the course of neurorehabilitation resulted in the significant (р < 0.001) decrease in hand spasticity and the increase in the total ABILHAND-Kids score and speech scores. No significant changes of these indicators were revealed in children of the comparison group. Beneficial effects of neurorehabilitation may be based on the enhanced plasticity of the neural circuits responsible for planning and execution of complex hand movements, as well as speech processes. The findings can be used to develop new methods for correction of motor and cognitive spheres in children with CP.
{"title":"Speech improvement in children with cerebral palsy by \"brain-computer-hand exoskeleton\" neurointerface rehabilitation","authors":"VB Pavlenko, S. Vlasenko, LS Orekhova, EA Biryukova","doi":"10.24075/brsmu.2023.026","DOIUrl":"https://doi.org/10.24075/brsmu.2023.026","url":null,"abstract":"As explained earlier, neurorehabilitation sessions involving the use of the non-invasive “brain – computer – hand exoskeleton” interface reduce hand muscle spasticity and improve motor skills in children with cerebral palsy (CP). However, the changes in the patients’ speech functions and their relationship with the upper limb mobility have not been analyzed. The study was aimed to assess the correlation between the motor and speech functions of children with CP, as well as to detect the changes in motor realization of speech production following complex treatment of patients including sessions of neurorehabilitation. The study involved children with CP aged 6–15. The index group (n = 40, 16 girls, 24 boys) received complex resort treatment with the course of neurorehabilitation, while the comparison group (n = 20, 10 girls, 10 boys) received standard resort treatment. A significant (р < 0.001) correlation between the total ABILHAND-Kids score and the indicators of speech production motor realization was revealed. In patients of the index group, complex treatment with the course of neurorehabilitation resulted in the significant (р < 0.001) decrease in hand spasticity and the increase in the total ABILHAND-Kids score and speech scores. No significant changes of these indicators were revealed in children of the comparison group. Beneficial effects of neurorehabilitation may be based on the enhanced plasticity of the neural circuits responsible for planning and execution of complex hand movements, as well as speech processes. The findings can be used to develop new methods for correction of motor and cognitive spheres in children with CP.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43103635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Gumenyuk, IA Zemlyanaya, A. Rami, ES Badula, AR Ismailov, NA Seroshtanov, SS Kokareva, AA Cheremisova, YuR Kupreichyuk
Today, the association of gut microbiota with external genital endometriosis (EGE) is of special scientific interest. The study was aimed to assess alterations of the gut microbiota taxonomic composition and explore their correlations with plasma levels of IL6, IL8 and TNFα at the species level in patients with EGE. The crosssectional comparative study involved 50 patients with EGE (index group) and 50 healthy women (control group). The changes in the gut microbiota taxonomic composition and plasma levels of IL6, IL8 and TNFα were assessed. A significant decrease in the abundance of such species, as Coprococcus catu (р = 0.009), Turicibacter sanguinis (р = 0.008) and Ruminococcus gnavus (р < 0.001), along with the increase in the abundance of Eubacterium ramulus (р = 0.040), Bacterioides dorei (р = 0.001), Prevotella divia (р = 0.008) and Shigella flexneri (р < 0.001) were found in the gut microbiota taxonomic composition in patients with EGE. Significant correlations between the IL6 levels and the abundance of Turicibacter sanguinis (r = –0.92; р = 0.001), IL8 levels and the abundance of Shigella flexneri (r = 0.72; р < 0.001), TNFα levels and the abundance of Prevotella divia (r = 0.77; р = 0.001) were revealed. The findings add to the available literature data on the features of gut microbiota alterations and their association with some inflammation biomarkers in individuals with EGE, which can justify further research in this area and probably open up new approaches to treatment of the disease.
{"title":"Gut microbiota alterations and their association with IL6, IL8 and TNFα levels in patients with external genital endometriosis","authors":"L. Gumenyuk, IA Zemlyanaya, A. Rami, ES Badula, AR Ismailov, NA Seroshtanov, SS Kokareva, AA Cheremisova, YuR Kupreichyuk","doi":"10.24075/brsmu.2023.018","DOIUrl":"https://doi.org/10.24075/brsmu.2023.018","url":null,"abstract":"Today, the association of gut microbiota with external genital endometriosis (EGE) is of special scientific interest. The study was aimed to assess alterations of the gut microbiota taxonomic composition and explore their correlations with plasma levels of IL6, IL8 and TNFα at the species level in patients with EGE. The crosssectional comparative study involved 50 patients with EGE (index group) and 50 healthy women (control group). The changes in the gut microbiota taxonomic composition and plasma levels of IL6, IL8 and TNFα were assessed. A significant decrease in the abundance of such species, as Coprococcus catu (р = 0.009), Turicibacter sanguinis (р = 0.008) and Ruminococcus gnavus (р < 0.001), along with the increase in the abundance of Eubacterium ramulus (р = 0.040), Bacterioides dorei (р = 0.001), Prevotella divia (р = 0.008) and Shigella flexneri (р < 0.001) were found in the gut microbiota taxonomic composition in patients with EGE. Significant correlations between the IL6 levels and the abundance of Turicibacter sanguinis (r = –0.92; р = 0.001), IL8 levels and the abundance of Shigella flexneri (r = 0.72; р < 0.001), TNFα levels and the abundance of Prevotella divia (r = 0.77; р = 0.001) were revealed. The findings add to the available literature data on the features of gut microbiota alterations and their association with some inflammation biomarkers in individuals with EGE, which can justify further research in this area and probably open up new approaches to treatment of the disease.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48203770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Guselnikova, V. Razenkova, D. Sufieva, D. Korzhevskii
Arterial hypertension is one of the most significant medical and social problems, being widespread and associated with the risk of renal failure, cardiovascular and cerebrovascular complications. The aim was to investigate the morphofunctional state of microglia in different regions of the rat brain in the setting of arterial hypertension. Brain samples from spontaneously hypertensive SHR rats aged 3–8 months (n = 4) were used as study material. Normotensive WKY rats of the same age (n = 3) were used as the control group. The work was performed using immunohistochemical analysis and confocal laser microscopy. During the quantitative analysis, we were seeking to determine the number of microgliocyte bodies and the area occupied by the bodies and processes of these cells per 1 mm2 of the nervous tissue. An immunohistochemical reaction for calcium-binding protein Iba1 revealed that in rats with arterial hypertension, microglia in the cerebral cortex, striatum, subcortical white matter and subfornical organ showed morphological signs of activation: increased body size and thickening of the processes of these cells. The strongest activation is demonstrated by microglia of the subfornical organ, which is in a preactivated state in normotensive rats. The performed statistical analysis revealed a trend towards an increase in the amount of microglia in the brain in SHR rats compared to animals in the control group. The Iba1/CD68 double immunofluorescence reaction showed no changes in the amount and/or distribution of lysosomal CD68 protein in spontaneously hypertensive rats compared to control group. The results obtained indicate chronic activation of microglia in the brain of spontaneously hypertensive rats. Activation of microglia in this case is not accompanied by an increase in the phagocytic activity of these cells.
{"title":"Activation of microglia in the brain of spontaneously hypertensive rats","authors":"V. Guselnikova, V. Razenkova, D. Sufieva, D. Korzhevskii","doi":"10.24075/brsmu.2023.024","DOIUrl":"https://doi.org/10.24075/brsmu.2023.024","url":null,"abstract":"Arterial hypertension is one of the most significant medical and social problems, being widespread and associated with the risk of renal failure, cardiovascular and cerebrovascular complications. The aim was to investigate the morphofunctional state of microglia in different regions of the rat brain in the setting of arterial hypertension. Brain samples from spontaneously hypertensive SHR rats aged 3–8 months (n = 4) were used as study material. Normotensive WKY rats of the same age (n = 3) were used as the control group. The work was performed using immunohistochemical analysis and confocal laser microscopy. During the quantitative analysis, we were seeking to determine the number of microgliocyte bodies and the area occupied by the bodies and processes of these cells per 1 mm2 of the nervous tissue. An immunohistochemical reaction for calcium-binding protein Iba1 revealed that in rats with arterial hypertension, microglia in the cerebral cortex, striatum, subcortical white matter and subfornical organ showed morphological signs of activation: increased body size and thickening of the processes of these cells. The strongest activation is demonstrated by microglia of the subfornical organ, which is in a preactivated state in normotensive rats. The performed statistical analysis revealed a trend towards an increase in the amount of microglia in the brain in SHR rats compared to animals in the control group. The Iba1/CD68 double immunofluorescence reaction showed no changes in the amount and/or distribution of lysosomal CD68 protein in spontaneously hypertensive rats compared to control group. The results obtained indicate chronic activation of microglia in the brain of spontaneously hypertensive rats. Activation of microglia in this case is not accompanied by an increase in the phagocytic activity of these cells.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49415010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EV Gantsgorn, OV Denisenko, YaO Osipenko, DA Kalmykova, AV Ivanov, S. Gerasyuta, G. Bulguryan, M. Ivanova, D. Saakyan
Type 1 diabetes mellitus is a condition caused by autoimmune damage to insulin–producing beta cells of the pancreatic islets, leading to endogenous insulin deficiency. Despite the sufficient knowledge of the disease and the availability of clinical recommendations for substitution therapy, the number of patients with this pathology is growing worldwide. At the same time, their cohort is very heterogeneous, including amid different etiology, concomitant genetic background, variations in the manifestation of the disease and severity. In this regard, traditional ideas about type 1 diabetes mellitus are being questioned, which requires special attention when managing patients with a clinical picture of the disease that differs from the traditional one. The article presents a clinical case of type 1 diabetes mellitus in a young patient, which demonstrates the importance of a personalized approach to the diagnosis and treatment of diabetic patients with a "non-classical" history.
{"title":"Type 1 diabetes mellitus: features of differential diagnosis","authors":"EV Gantsgorn, OV Denisenko, YaO Osipenko, DA Kalmykova, AV Ivanov, S. Gerasyuta, G. Bulguryan, M. Ivanova, D. Saakyan","doi":"10.24075/brsmu.2023.023","DOIUrl":"https://doi.org/10.24075/brsmu.2023.023","url":null,"abstract":"Type 1 diabetes mellitus is a condition caused by autoimmune damage to insulin–producing beta cells of the pancreatic islets, leading to endogenous insulin deficiency. Despite the sufficient knowledge of the disease and the availability of clinical recommendations for substitution therapy, the number of patients with this pathology is growing worldwide. At the same time, their cohort is very heterogeneous, including amid different etiology, concomitant genetic background, variations in the manifestation of the disease and severity. In this regard, traditional ideas about type 1 diabetes mellitus are being questioned, which requires special attention when managing patients with a clinical picture of the disease that differs from the traditional one. The article presents a clinical case of type 1 diabetes mellitus in a young patient, which demonstrates the importance of a personalized approach to the diagnosis and treatment of diabetic patients with a \"non-classical\" history.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47559722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZK Batyrova, AS Bolshakova, Z. Kumykova, DA Kruglyak, E. Uvarova, VD Chuprynin, FSh Mamedova, I. Sadelov, D. Trofimov
Two forms of Mayer–Rokitansky–Kuster–Hauser (MRKH) syndrome are recognized: isolated uterovaginal agenesis and associated with extragenital malformations, including several well-recognized syndromes. Trichorhinophalangeal syndrome (TRPS) is a rare autosomal dominant condition characterized by facial dysmorphism, ectodermal and skeletal features. TRPS comprises TRPSI (caused by a heterozygous pathogenic variant in TRPS1), TRPSII (caused by contiguous gene deletion of TRPS1, RAD21, and EXT1). Genital anomalies occur particularly in TRPSII. We present a case of rare combination TRPSII with MRKH syndrome. Delayed diagnosis resulted to prolonged pain syndrome and repeated surgery. Recognition of genital anomalies in TRPS allows timely referral diagnosis and appropriate care by paediatrician and adolescent gynaecologists.
{"title":"A rare case of combination trichorinophalangeal syndrome and Mayer-Rokitansky-Küster-Hauser syndrome","authors":"ZK Batyrova, AS Bolshakova, Z. Kumykova, DA Kruglyak, E. Uvarova, VD Chuprynin, FSh Mamedova, I. Sadelov, D. Trofimov","doi":"10.24075/brsmu.2023.022","DOIUrl":"https://doi.org/10.24075/brsmu.2023.022","url":null,"abstract":"Two forms of Mayer–Rokitansky–Kuster–Hauser (MRKH) syndrome are recognized: isolated uterovaginal agenesis and associated with extragenital malformations, including several well-recognized syndromes. Trichorhinophalangeal syndrome (TRPS) is a rare autosomal dominant condition characterized by facial dysmorphism, ectodermal and skeletal features. TRPS comprises TRPSI (caused by a heterozygous pathogenic variant in TRPS1), TRPSII (caused by contiguous gene deletion of TRPS1, RAD21, and EXT1). Genital anomalies occur particularly in TRPSII. We present a case of rare combination TRPSII with MRKH syndrome. Delayed diagnosis resulted to prolonged pain syndrome and repeated surgery. Recognition of genital anomalies in TRPS allows timely referral diagnosis and appropriate care by paediatrician and adolescent gynaecologists.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":"23 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68986922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Vasileva, VP Tereschenko, BN Krapivin, A. Muslimov, IS Kukushkin, II Pateev, S. Rybtsov, RA Ivanov, VV Reshetnikov
Development of the new tuberculosis vaccines that would be effective in adults is an urgent task: worldwide, the annual death toll of this disease exceeds 1.5 million. In the recent decades, the matter has been addressed in numerous studies, but none has yielded an effective vaccine so far. There are many factors to resistance against tuberculosis; this study focuses on the T-cell response, a mechanism that enables elimination of intracellular pathogens, such as M. tuberculosis. We aimed to develop an mRNA vaccine capable of triggering a pronounced T-cell response to the M. tuberculosis antigens. The in silico analysis allowed us to select epitopes of the M. tuberculosis secreted protein ESAT6 (Rv3875) and design a multi-epitope mRNA vaccine thereon. We assessed the intensity of T-cell response in mice immunized with mRNA vaccines that encode a full-length or multi-epitope antigen. The results of this study in mice show that immunization with a multi-epitope mRNA vaccine produces twice as many IFNγ-secreting splenocytes in response to specific stimulation than immunization with an mRNA vaccine encoding the full-length protein. Thus, the developed multi-epitope mRNA vaccine can be an effective M. tuberculosis prevention agent the mode of action of which involves formation of a pronounced T-cell response.
{"title":"Immunogenicity of full-length and multi-epitope mRNA vaccines for M. Tuberculosis as demonstrated by the intensity of T-cell response: a comparative study in mice","authors":"O. Vasileva, VP Tereschenko, BN Krapivin, A. Muslimov, IS Kukushkin, II Pateev, S. Rybtsov, RA Ivanov, VV Reshetnikov","doi":"10.24075/brsmu.2023.021","DOIUrl":"https://doi.org/10.24075/brsmu.2023.021","url":null,"abstract":"Development of the new tuberculosis vaccines that would be effective in adults is an urgent task: worldwide, the annual death toll of this disease exceeds 1.5 million. In the recent decades, the matter has been addressed in numerous studies, but none has yielded an effective vaccine so far. There are many factors to resistance against tuberculosis; this study focuses on the T-cell response, a mechanism that enables elimination of intracellular pathogens, such as M. tuberculosis. We aimed to develop an mRNA vaccine capable of triggering a pronounced T-cell response to the M. tuberculosis antigens. The in silico analysis allowed us to select epitopes of the M. tuberculosis secreted protein ESAT6 (Rv3875) and design a multi-epitope mRNA vaccine thereon. We assessed the intensity of T-cell response in mice immunized with mRNA vaccines that encode a full-length or multi-epitope antigen. The results of this study in mice show that immunization with a multi-epitope mRNA vaccine produces twice as many IFNγ-secreting splenocytes in response to specific stimulation than immunization with an mRNA vaccine encoding the full-length protein. Thus, the developed multi-epitope mRNA vaccine can be an effective M. tuberculosis prevention agent the mode of action of which involves formation of a pronounced T-cell response.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44648314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VD Nazarov, C. Cherebillo, S. Lapin, DV Sidorenko, YA Devyatkina, AC Musonova, TV Petrova, AI Nikiforova, A. Ivanova
Spinal muscular atrophy (SMA) is an inherited neuromuscular disease characterized by progressive skeletal muscular weakness and atrophy. The newborn screening for spinal muscular atrophy should define all molecular forms of SMA. The aim of this study is to compare a PCR-based test for detection of homozygous SMN1 loss with multiple ligation probe amplification (MPLA) in patients with spinal muscular atrophy and other numerical changes of the SMN1 gene. PCR-based test was used to detect exon 7 of SMN1 gene homozygous loss. The study included 341 samples of patients with clinical suspicion of SMA from Biobank of Centre of Molecular Medicine of Pavlov State Medical University (Saint-Petersburg, Russia). Group 1 included 206 whole blood samples and Group 2 included 135 dried blood spot (DBS) samples. Copy number of the SMN1 and SMN2 genes had been evaluated with MLPA as a reference method. The results showed that kit was able to detect homozygous SMN1 loss in all samples from group 1 and 2 (Group 1: n = 67; 33%; Group 2: n = 19; 14%). At the same time in all samples with 1–3 copies of the SMN1 gene, the results of the kit were negative for homozygous loss of SMN1 gene (Group 1: n = 139; 67%; Group 2: n = 116; 86%). Kit showed high effectiveness in the detection of homozygous loss SMN1 gene. The kit detects all possible molecular forms of homozygous SMN1 gene loss in both DNA samples extracted from the whole blood and DBS.
{"title":"Detection of SMN1 loss with PCR-based screening test","authors":"VD Nazarov, C. Cherebillo, S. Lapin, DV Sidorenko, YA Devyatkina, AC Musonova, TV Petrova, AI Nikiforova, A. Ivanova","doi":"10.24075/brsmu.2023.025","DOIUrl":"https://doi.org/10.24075/brsmu.2023.025","url":null,"abstract":"Spinal muscular atrophy (SMA) is an inherited neuromuscular disease characterized by progressive skeletal muscular weakness and atrophy. The newborn screening for spinal muscular atrophy should define all molecular forms of SMA. The aim of this study is to compare a PCR-based test for detection of homozygous SMN1 loss with multiple ligation probe amplification (MPLA) in patients with spinal muscular atrophy and other numerical changes of the SMN1 gene. PCR-based test was used to detect exon 7 of SMN1 gene homozygous loss. The study included 341 samples of patients with clinical suspicion of SMA from Biobank of Centre of Molecular Medicine of Pavlov State Medical University (Saint-Petersburg, Russia). Group 1 included 206 whole blood samples and Group 2 included 135 dried blood spot (DBS) samples. Copy number of the SMN1 and SMN2 genes had been evaluated with MLPA as a reference method. The results showed that kit was able to detect homozygous SMN1 loss in all samples from group 1 and 2 (Group 1: n = 67; 33%; Group 2: n = 19; 14%). At the same time in all samples with 1–3 copies of the SMN1 gene, the results of the kit were negative for homozygous loss of SMN1 gene (Group 1: n = 139; 67%; Group 2: n = 116; 86%). Kit showed high effectiveness in the detection of homozygous loss SMN1 gene. The kit detects all possible molecular forms of homozygous SMN1 gene loss in both DNA samples extracted from the whole blood and DBS.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48792123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Koniaeva, E. Varakuta, A. Leiman, DO Rafiev, EN Bolbasov, Ksenia S. Stankevich
Wound defects of the oral mucosa are a common pathology the treatment of which often involves synthetic membranes. Development of varieties of such membranes is an ongoing process. This study aimed to register morphological features of the oral mucosa regeneration process in the presence of one of the varieties, the polymer piezoelectric membranes. The study involved 45 Wistar rats divided into 3 groups: 1) animals with an open wound defect; 2) animals with a wound defect covered with a copper-coated polymer membrane; 3) intact animals. The samples for morphometric study were collected on the 3rd, 7th and 12th days. On the 3rd day, rats of group 1 had the specific area of granulation tissue 1.4 times greater than that in group 2 (p = 0.033). In group 1 rats, endotheliocytes expressed more VEGF than in the animals of group 2. In group 2, the defect was ultimately completely covered with the epithelial layer, which was not the case in group 1. On the 7th day, the epithelium in rats of group 2 was twice as thick as the layer registered in group 1 (p = 0.019). Granulation tissue was replaced by loose fibrous connective tissue. In group 1, the specific area of inflammatory infiltration was greater than that of loose fibrous connective tissue, and the VEGF expression level was lower than in group 2. On the 12th day, the predominant tissue in group 2 was the loose fibrous connective tissue, the VEGF expression level equaled that of group 3, and peripheral nerves began to grow. In group 1, the specific area of dense fibrous tissue was 3.9 times greater than that in group 2 (p = 0.012), the epithelium had pathological changes and the VEGF expression was below control values. Thus, a polymer piezoelectric membrane had a positive effect on the post-wound restoration of the oral mucosa tissues.
{"title":"Morphological peculiarities of regeneration of oral mucosa associated with use of polymeric piezoelectric membranes","authors":"A. Koniaeva, E. Varakuta, A. Leiman, DO Rafiev, EN Bolbasov, Ksenia S. Stankevich","doi":"10.24075/brsmu.2023.020","DOIUrl":"https://doi.org/10.24075/brsmu.2023.020","url":null,"abstract":"Wound defects of the oral mucosa are a common pathology the treatment of which often involves synthetic membranes. Development of varieties of such membranes is an ongoing process. This study aimed to register morphological features of the oral mucosa regeneration process in the presence of one of the varieties, the polymer piezoelectric membranes. The study involved 45 Wistar rats divided into 3 groups: 1) animals with an open wound defect; 2) animals with a wound defect covered with a copper-coated polymer membrane; 3) intact animals. The samples for morphometric study were collected on the 3rd, 7th and 12th days. On the 3rd day, rats of group 1 had the specific area of granulation tissue 1.4 times greater than that in group 2 (p = 0.033). In group 1 rats, endotheliocytes expressed more VEGF than in the animals of group 2. In group 2, the defect was ultimately completely covered with the epithelial layer, which was not the case in group 1. On the 7th day, the epithelium in rats of group 2 was twice as thick as the layer registered in group 1 (p = 0.019). Granulation tissue was replaced by loose fibrous connective tissue. In group 1, the specific area of inflammatory infiltration was greater than that of loose fibrous connective tissue, and the VEGF expression level was lower than in group 2. On the 12th day, the predominant tissue in group 2 was the loose fibrous connective tissue, the VEGF expression level equaled that of group 3, and peripheral nerves began to grow. In group 1, the specific area of dense fibrous tissue was 3.9 times greater than that in group 2 (p = 0.012), the epithelium had pathological changes and the VEGF expression was below control values. Thus, a polymer piezoelectric membrane had a positive effect on the post-wound restoration of the oral mucosa tissues.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46427428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Rogova, DS Lipov, V. Perfilova, M. Kustova, AV Mukhina, Danila A. Churzin
Granulosa cells feed the oocyte during its maturation and protect it. Aberrant apoptosis in these cells is known to ultimately impair oogenesis. The current knowledge of how extragenital inflammation affects apoptosis in granulosa cells is incomprehensive, which is the root of an urgent problem connected to the spread of inflammatory diseases and the growing level of female infertility. This study aimed to assess the intensity of granulosa cell apoptosis in women with impaired reproductive function that suffer from chronic respiratory and/or digestive system diseases of inflammatory origin, and to identify the link, if any, between the studied factor and dysfunction of the reproductive system in the test group. The group included 60 women with a history of respiratory and/or digestive system inflammatory pathology that underwent IVF in 2021–2022. The women were donors of the granulosa cells from the follicular fluid collected through transvaginal puncture of preovulatory follicles. We studied the apoptosis process with the help of flow cytometry. For statistical analysis, we used the Fisher's F-test and the Kruskal–Wallis test. Twenty participants without extragenital pathology in their medical histories, the first subgroup, had the level of apoptosis in granulosa cells at 0.0088 ± 0.0062%, which is significantly lower than in twenty donors with a history of chronic inflammatory digestive system diseases, the second subgroup (granulosa cell apoptosis at 0.0140 ± 0.0099%, p = 0.015), and the subgroup of women suffering from inflammatory diseases of the respiratory system (granulosa cell apoptosis at 0.0650 ± 0.0391%, p = 0.033); the efficacy of IVF was higher in the first subgroup.
{"title":"Apoptosis of granulosa cells in women with impaired reproductive function and extragenital pathology","authors":"L. Rogova, DS Lipov, V. Perfilova, M. Kustova, AV Mukhina, Danila A. Churzin","doi":"10.24075/brsmu.2023.019","DOIUrl":"https://doi.org/10.24075/brsmu.2023.019","url":null,"abstract":"Granulosa cells feed the oocyte during its maturation and protect it. Aberrant apoptosis in these cells is known to ultimately impair oogenesis. The current knowledge of how extragenital inflammation affects apoptosis in granulosa cells is incomprehensive, which is the root of an urgent problem connected to the spread of inflammatory diseases and the growing level of female infertility. This study aimed to assess the intensity of granulosa cell apoptosis in women with impaired reproductive function that suffer from chronic respiratory and/or digestive system diseases of inflammatory origin, and to identify the link, if any, between the studied factor and dysfunction of the reproductive system in the test group. The group included 60 women with a history of respiratory and/or digestive system inflammatory pathology that underwent IVF in 2021–2022. The women were donors of the granulosa cells from the follicular fluid collected through transvaginal puncture of preovulatory follicles. We studied the apoptosis process with the help of flow cytometry. For statistical analysis, we used the Fisher's F-test and the Kruskal–Wallis test. Twenty participants without extragenital pathology in their medical histories, the first subgroup, had the level of apoptosis in granulosa cells at 0.0088 ± 0.0062%, which is significantly lower than in twenty donors with a history of chronic inflammatory digestive system diseases, the second subgroup (granulosa cell apoptosis at 0.0140 ± 0.0099%, p = 0.015), and the subgroup of women suffering from inflammatory diseases of the respiratory system (granulosa cell apoptosis at 0.0650 ± 0.0391%, p = 0.033); the efficacy of IVF was higher in the first subgroup.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44691157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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