IV Zapesotskaya, MV Sokolyskaya, TN Razuvaeva, SL Borisova
The study of neuropsychological features that cause eating disorders may provide a starting point for planning complex studies that allow for integral assessment of the internal and external mechanisms and patterns of eating disorders. The work aims to evaluate the influence of the neuropsychological status on features of the body schema in eating disorders. We conducted an analysis of the subjective and objective indicators of the body image on 51 women aged 20–35 years using face-relative hand position reproduction tests, the "Silhouette" method, measurement of the right hand index finger diameter and of the foot length, and a self-image questionnaire. We carried out qualitative and quantitative assessment of the neuropsychological status using the Luriev test battery. For the analysis of control functions, we used the Wisconsin sorting card test, Cantidad-Numér interference task (Canum), and "Block Span". We found that women with atypical eating behaviors noted the following features associated with a subjective attitude towards their own body: prevalence of dissatisfaction in one's emotional evaluation due to the perception of one's own appearance, stemming from the beliefs and ideas about one's ideal appearance, despite the absence of the abnormalities associated with the objectified ideas of one's own body (weight, size, body proportions). We identified modal-nonspecific control function deficiencies characteristic of different types of eating disorders.
{"title":"Influence of neuropsychological status on body schema in eating disorders","authors":"IV Zapesotskaya, MV Sokolyskaya, TN Razuvaeva, SL Borisova","doi":"10.24075/brsmu.2022.051","DOIUrl":"https://doi.org/10.24075/brsmu.2022.051","url":null,"abstract":"The study of neuropsychological features that cause eating disorders may provide a starting point for planning complex studies that allow for integral assessment of the internal and external mechanisms and patterns of eating disorders. The work aims to evaluate the influence of the neuropsychological status on features of the body schema in eating disorders. We conducted an analysis of the subjective and objective indicators of the body image on 51 women aged 20–35 years using face-relative hand position reproduction tests, the \"Silhouette\" method, measurement of the right hand index finger diameter and of the foot length, and a self-image questionnaire. We carried out qualitative and quantitative assessment of the neuropsychological status using the Luriev test battery. For the analysis of control functions, we used the Wisconsin sorting card test, Cantidad-Numér interference task (Canum), and \"Block Span\". We found that women with atypical eating behaviors noted the following features associated with a subjective attitude towards their own body: prevalence of dissatisfaction in one's emotional evaluation due to the perception of one's own appearance, stemming from the beliefs and ideas about one's ideal appearance, despite the absence of the abnormalities associated with the objectified ideas of one's own body (weight, size, body proportions). We identified modal-nonspecific control function deficiencies characteristic of different types of eating disorders.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42328869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Balanovska, I. Gorin, V. Petrushenko, DK Chernevskiy, S. Koshel, I. Temirbulatov, VYu Pylev, AT Agdzhoyan
The correlation between the risk of death from COVID-19 and the patient's ethnogeographic origin has been previously detected. LZTFL1 gene was identified as a potential marker of a two times higher risk of severe COVID-19. The study was aimed to assess spatial variation in the LZTFL1 SNP markers in indigenous populations of Russia and the world. Spatial variation in the LZTFL1 polymorphic markers was analyzed in 28 metapopulations (97 ethnic groups) of North Eurasia (n = 1980) and 34 world's metapopulations (n = 3637) by bioinformatics, statistical and cartographic methods. In North Eurasia, the major geographic variation vectors, North–South and West–East, are generally in line with the Caucasoid–Mongoloid anthropological vector. Global variation also corresponds to anthropological features: each cluster of indigenous populations includes only those from the place where it originates: Africa, Asia, or America. Indo-European cluster integrates Caucasoid populations of Europe and Asia. All four clusters of the world's indigenous population are separated from each other. The huge genetic diversity of Russia peoples and neighboring countries forms a bridge between three continents: Europe, Asia and America. Cartographic atlas for spatial variation in 11 LZTFL1 markers in the populations has been created. The following major patterns have been revealed: а) the world's extrema fall on the indigenous populations of Africa and America; 2) Eurasia constitutes a transition zone between these two extrema, but has its own patterns and shows enormous scale of variation shows enormous variation on a global scale; 3) the genetic landscape of Russia tends to be seamlessly integrated into the Eurasian landscape.
{"title":"Geographic distribution of the LZTFL1 SNP markers associated with severe COVID-19 in Russia and worldwide","authors":"E. Balanovska, I. Gorin, V. Petrushenko, DK Chernevskiy, S. Koshel, I. Temirbulatov, VYu Pylev, AT Agdzhoyan","doi":"10.24075/brsmu.2022.047","DOIUrl":"https://doi.org/10.24075/brsmu.2022.047","url":null,"abstract":"The correlation between the risk of death from COVID-19 and the patient's ethnogeographic origin has been previously detected. LZTFL1 gene was identified as a potential marker of a two times higher risk of severe COVID-19. The study was aimed to assess spatial variation in the LZTFL1 SNP markers in indigenous populations of Russia and the world. Spatial variation in the LZTFL1 polymorphic markers was analyzed in 28 metapopulations (97 ethnic groups) of North Eurasia (n = 1980) and 34 world's metapopulations (n = 3637) by bioinformatics, statistical and cartographic methods. In North Eurasia, the major geographic variation vectors, North–South and West–East, are generally in line with the Caucasoid–Mongoloid anthropological vector. Global variation also corresponds to anthropological features: each cluster of indigenous populations includes only those from the place where it originates: Africa, Asia, or America. Indo-European cluster integrates Caucasoid populations of Europe and Asia. All four clusters of the world's indigenous population are separated from each other. The huge genetic diversity of Russia peoples and neighboring countries forms a bridge between three continents: Europe, Asia and America. Cartographic atlas for spatial variation in 11 LZTFL1 markers in the populations has been created. The following major patterns have been revealed: а) the world's extrema fall on the indigenous populations of Africa and America; 2) Eurasia constitutes a transition zone between these two extrema, but has its own patterns and shows enormous scale of variation shows enormous variation on a global scale; 3) the genetic landscape of Russia tends to be seamlessly integrated into the Eurasian landscape.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47721421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NK Abdraimova, M. Kornienko, D. Bespiatykh, NS Kuptsov, R. Gorodnichev, E. Shitikov
Currently, the search for new therapy options for infectious diseases caused by multidrug-resistant Staphylococcus aureus is a priority. Combining antibiotics with virulent (lytic) bacteriophages may be considered a viable alternative to conventional antibiotic therapy. The study was aimed to assess the combined effects of the lytic bacteriophage vB_SauM-515A1 of Herelleviridae family and antibiotics of various classes on the Staphylococcus aureus clinical strains. Strains (n = 4) belong to the clinically significant sequence types ST1, ST8, ST121 and are characterized by multidrug resistance. Efficiency of the combination use of two antibacterial agents was assessed by comparison of optical densities of the test samples and controls after 24 hrs. of incubation. Mutually enhancing activities of bacteriophage used in combination with oxacillin, tetracycline and linezolid were revealed, in contrast to the separate use of each agent. Efficiency generally increased with the selected optimum multiplicity of infection values. No antagonism was revealed when combining the phage with antibiotics. Thus, virulent bacteriophage vB_SauM515A1 can be considered as a possible auxiliary therapeutic agent for antimicrobial-resistant strains of Staphylococcus aureus.
{"title":"Combined effects of bacteriophage vB_SauM-515A1 and antibiotics on the Staphylococcus aureus clinical isolates","authors":"NK Abdraimova, M. Kornienko, D. Bespiatykh, NS Kuptsov, R. Gorodnichev, E. Shitikov","doi":"10.24075/brsmu.2022.052","DOIUrl":"https://doi.org/10.24075/brsmu.2022.052","url":null,"abstract":"Currently, the search for new therapy options for infectious diseases caused by multidrug-resistant Staphylococcus aureus is a priority. Combining antibiotics with virulent (lytic) bacteriophages may be considered a viable alternative to conventional antibiotic therapy. The study was aimed to assess the combined effects of the lytic bacteriophage vB_SauM-515A1 of Herelleviridae family and antibiotics of various classes on the Staphylococcus aureus clinical strains. Strains (n = 4) belong to the clinically significant sequence types ST1, ST8, ST121 and are characterized by multidrug resistance. Efficiency of the combination use of two antibacterial agents was assessed by comparison of optical densities of the test samples and controls after 24 hrs. of incubation. Mutually enhancing activities of bacteriophage used in combination with oxacillin, tetracycline and linezolid were revealed, in contrast to the separate use of each agent. Efficiency generally increased with the selected optimum multiplicity of infection values. No antagonism was revealed when combining the phage with antibiotics. Thus, virulent bacteriophage vB_SauM515A1 can be considered as a possible auxiliary therapeutic agent for antimicrobial-resistant strains of Staphylococcus aureus.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41804805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Andreevskaya, TG Smirnova, L. Chernousova, E. Larionova, EA Kiseleva, A. Ergeshov
Fluoroquinolones are the main group of drugs used for treatment of multidrug resistant tuberculosis (MDR-TB). The study was aimed to assess the diversity of mutation in the gyrA gene and to evaluate the association of gyrA mutations with the phenotypic resistance to levofloxacin and the general drug resistance profile of the pathogen. The study involved assessment of diagnostic materials obtained from 2836 patients with pulmonary tuberculosis. TB-BIOCHIP-2 and AmplitubeFQ-RV kits were used for identification of the gyrA mutations. Phenotypic drug susceptibility of M. tuberculosis (MTB) was defined using the BACTEC MGIT 960 test system. It was shown that mutations D94G (41.63%; 95% CI: 38.03–45.32%) and A90V (21.32%; 95% CI: 18.44–24.50%) prevailed in MBT, although some isolates carrying these mutations were obtained from the newly diagnosed patients with pulmonary tuberculosis. It was found that mutation D94A was not strongly associated with the phenotypic resistance to fluoroquinolones. Fluoroquinolone resistance was usually associated with multiple drug resistance (93.52%; 95% CI 91.43–95.12%). In 2.31% (95% CI 1.78–3.00%) of cases, genotypic heteroresistance to fluoroquinolones was detected: mixed populations included 2–4 MTB pools with various structure of the gyrA QRDR. The results obtained lead to the conclusion that resistance to fluoroquinolones that is usually associated with the existing MDR arises in the modern MTB population. MTB carrying gyrA mutations D94G and A90V seems to be the most promising in evolutionary terms.
氟喹诺酮类药物是治疗耐多药结核病(MDR-TB)的主要药物。该研究旨在评估gyrA基因突变的多样性，并评估gyrA突变与左氧氟沙星表型耐药和病原菌一般耐药谱的关系。该研究评估了2836例肺结核患者的诊断资料。使用TB-BIOCHIP-2和AmplitubeFQ-RV试剂盒鉴定gyrA突变。采用BACTEC MGIT 960检测系统确定结核分枝杆菌(MTB)的表型药敏。结果表明，突变D94G (41.63%;95% CI: 38.03-45.32%)和A90V (21.32%;95% CI: 18.44-24.50%)在MBT中普遍存在，尽管从新诊断的肺结核患者中获得了一些携带这些突变的分离株。发现突变D94A与氟喹诺酮类药物的表型耐药无明显相关性。氟喹诺酮类药物耐药多伴有多重耐药(93.52%;95% ci 91.43-95.12%)。2.31% (95% CI 1.78 ~ 3.00%)的病例检测到氟喹诺酮类药物基因型异耐药:混合人群包括2-4个具有不同gyrA QRDR结构的MTB池。所获得的结果得出的结论是，对氟喹诺酮类药物的耐药性通常与现有的耐多药有关，在现代MTB人群中出现。从进化角度来看，携带gyrA突变D94G和A90V的MTB似乎是最有希望的。
{"title":"The nature of genotypic resistance to fluoroquinolones in Mycobacterium tuberculosis circulating in Russian Federation","authors":"S. Andreevskaya, TG Smirnova, L. Chernousova, E. Larionova, EA Kiseleva, A. Ergeshov","doi":"10.24075/brsmu.2022.054","DOIUrl":"https://doi.org/10.24075/brsmu.2022.054","url":null,"abstract":"Fluoroquinolones are the main group of drugs used for treatment of multidrug resistant tuberculosis (MDR-TB). The study was aimed to assess the diversity of mutation in the gyrA gene and to evaluate the association of gyrA mutations with the phenotypic resistance to levofloxacin and the general drug resistance profile of the pathogen. The study involved assessment of diagnostic materials obtained from 2836 patients with pulmonary tuberculosis. TB-BIOCHIP-2 and AmplitubeFQ-RV kits were used for identification of the gyrA mutations. Phenotypic drug susceptibility of M. tuberculosis (MTB) was defined using the BACTEC MGIT 960 test system. It was shown that mutations D94G (41.63%; 95% CI: 38.03–45.32%) and A90V (21.32%; 95% CI: 18.44–24.50%) prevailed in MBT, although some isolates carrying these mutations were obtained from the newly diagnosed patients with pulmonary tuberculosis. It was found that mutation D94A was not strongly associated with the phenotypic resistance to fluoroquinolones. Fluoroquinolone resistance was usually associated with multiple drug resistance (93.52%; 95% CI 91.43–95.12%). In 2.31% (95% CI 1.78–3.00%) of cases, genotypic heteroresistance to fluoroquinolones was detected: mixed populations included 2–4 MTB pools with various structure of the gyrA QRDR. The results obtained lead to the conclusion that resistance to fluoroquinolones that is usually associated with the existing MDR arises in the modern MTB population. MTB carrying gyrA mutations D94G and A90V seems to be the most promising in evolutionary terms.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41825009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Gavisova, NN Stenyaeva, ZR Gardanova, T. Nazarenko, N. Dolgushina
Androgens play a key role in the physiology of the female body and the reproductive system. Androgen receptor expression in the various tissues points to the importance of androgens in the regulation of the female sexual and social functioning. The study aimed to evaluate sexual functioning in women with infertility and diminished ovarian reserve (DOR) using the Female Sexual Functioning Index questionnaire (FSFI). A cross-sectional study of 496 patients with infertility and DOR assessed the degree of sexual dysfunction in conjunction with the changes in the androgenic profiles as indicated by the androstenedione levels in the blood serum. Women with infertility and DOR were significantly more likely to report changes in sexual functioning, including a decrease in libido and in the quality and frequency of sexual relations. Furthermore, patients with normal androstenedione levels generally significantly outscored patients with decreased androstenedione levels (average questionnaire scores 21.2 ± 7.2 and 15.17 ± 3.0 respectively), indicating a lesser degree of sexual dysfunction in the former group; on the other hand, the latter group reported increased pain and decreased attraction, arousal, lubrication, orgasm, and satisfaction. Hormonal profile changes in patients with DOR, including decreased androstenedione levels, significantly impact sexual functioning, and their detection in clinical practice will allow to objectify complaints at an earlier state in order to assess the severity of sexual dysfunction and determine further personalized management tactics.
{"title":"Changes in sexual functioning in women of reproductive age with infertility and diminished ovarian reserve","authors":"A. Gavisova, NN Stenyaeva, ZR Gardanova, T. Nazarenko, N. Dolgushina","doi":"10.24075/brsmu.2022.045","DOIUrl":"https://doi.org/10.24075/brsmu.2022.045","url":null,"abstract":"Androgens play a key role in the physiology of the female body and the reproductive system. Androgen receptor expression in the various tissues points to the importance of androgens in the regulation of the female sexual and social functioning. The study aimed to evaluate sexual functioning in women with infertility and diminished ovarian reserve (DOR) using the Female Sexual Functioning Index questionnaire (FSFI). A cross-sectional study of 496 patients with infertility and DOR assessed the degree of sexual dysfunction in conjunction with the changes in the androgenic profiles as indicated by the androstenedione levels in the blood serum. Women with infertility and DOR were significantly more likely to report changes in sexual functioning, including a decrease in libido and in the quality and frequency of sexual relations. Furthermore, patients with normal androstenedione levels generally significantly outscored patients with decreased androstenedione levels (average questionnaire scores 21.2 ± 7.2 and 15.17 ± 3.0 respectively), indicating a lesser degree of sexual dysfunction in the former group; on the other hand, the latter group reported increased pain and decreased attraction, arousal, lubrication, orgasm, and satisfaction. Hormonal profile changes in patients with DOR, including decreased androstenedione levels, significantly impact sexual functioning, and their detection in clinical practice will allow to objectify complaints at an earlier state in order to assess the severity of sexual dysfunction and determine further personalized management tactics.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46285162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VS Okovantsev, G. Ponomarev, A. Agdzhoyan, A. T. Agdzhoyan, VYu Pylev, E. Balanovska
The peculiarity of the Russian North gene pool has long become scientific fact, but has yet to receive informative explanation. Genetic drift cannot be the only contributing factor in the striking genetic differences between not only northern Russian populations and the southern ones, but among individual northern populations as well. Studying Russian North gene pools previously underrepresented in scientific literature may help understand this phenomenon. The work aimed to perform a subtotal study of the gene pool of the Arkhangelsk Oblast Pomors (Onega Coast, Summer Coast, the western fragment of the Winter Coast; n = 130) using a panel of 60 Y-chromosome SNP markers through multidimensional scaling and mapping of genetic distances. The frequencies of 14 identified haplogroups differ drastically in Pomor populations: haplogroups I1, R1a, and N3 each comprise a quarter of the total Pomor gene pool, I2-P37.2, and R1b each comprise about 8%, and the rest of the haplogroups are rare. The Onega Coast Pomors showed genetic similarity to a wide range of North-Eastern Europe Finnic-speaking populations, as well as to Russian populations with a strong pre-Slavic substratum. The Summer Coast Pomors are close to the Scandinavian gene pools, and the Winter Coast Pomors are similar only to specific Finn and Swede populations. None of the Pomor populations demonstrate genetic similarity with the Novgorod Oblast Russian populations, with which the origin of the Pomors is traditionally associated. The genetic distances between Pomor populations are so great, they are comparable to the general range of variability between the Eastern Slavic, Baltic, and Finno-Ugric peoples of the region. The reasons for such pronounced originality of Pomor populations presumably include, along with genetic drift, the gene pool of each population being underlied by a different pre-Slavic substrate, with later gene flows as an additional factor.
{"title":"Peculiarity of Pomors of Onega Peninsula and Winter Coast in the genetic context of Northern Europe","authors":"VS Okovantsev, G. Ponomarev, A. Agdzhoyan, A. T. Agdzhoyan, VYu Pylev, E. Balanovska","doi":"10.24075/brsmu.2022.046","DOIUrl":"https://doi.org/10.24075/brsmu.2022.046","url":null,"abstract":"The peculiarity of the Russian North gene pool has long become scientific fact, but has yet to receive informative explanation. Genetic drift cannot be the only contributing factor in the striking genetic differences between not only northern Russian populations and the southern ones, but among individual northern populations as well. Studying Russian North gene pools previously underrepresented in scientific literature may help understand this phenomenon. The work aimed to perform a subtotal study of the gene pool of the Arkhangelsk Oblast Pomors (Onega Coast, Summer Coast, the western fragment of the Winter Coast; n = 130) using a panel of 60 Y-chromosome SNP markers through multidimensional scaling and mapping of genetic distances. The frequencies of 14 identified haplogroups differ drastically in Pomor populations: haplogroups I1, R1a, and N3 each comprise a quarter of the total Pomor gene pool, I2-P37.2, and R1b each comprise about 8%, and the rest of the haplogroups are rare. The Onega Coast Pomors showed genetic similarity to a wide range of North-Eastern Europe Finnic-speaking populations, as well as to Russian populations with a strong pre-Slavic substratum. The Summer Coast Pomors are close to the Scandinavian gene pools, and the Winter Coast Pomors are similar only to specific Finn and Swede populations. None of the Pomor populations demonstrate genetic similarity with the Novgorod Oblast Russian populations, with which the origin of the Pomors is traditionally associated. The genetic distances between Pomor populations are so great, they are comparable to the general range of variability between the Eastern Slavic, Baltic, and Finno-Ugric peoples of the region. The reasons for such pronounced originality of Pomor populations presumably include, along with genetic drift, the gene pool of each population being underlied by a different pre-Slavic substrate, with later gene flows as an additional factor.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43818539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Poydasheva, I. Bakulin, D. Sinitsyn, A. Zabirova, N. Suponeva, NV Maslenikov, EE Tsukarzi, S. Mosolov, M. Piradov
Stanford neuromodulation therapy (SNT) is the state-of-the-art magnetic stimulation protocol that has been developed for management of treatment-resistant depression (TRD). The study was aimed to assess the possibility of SNT implementation in clinical practice and to define the protocol safety and efficacy in patients with TRD being an episode of the recurrent depressive disorder or bipolar disorder at the independent center. The study involved six patients (among them three women aged 21–66) with TRD associated with recurrent depression and type 1 or 2 bipolar disorder. The patients received intermittent theta-burst stimulation in accordance with the SNT protocol for five days: applying 10 triple blocks of stimulation daily at intervals of 1 hr between the blocks to the selected stimulation site showing maximum negative functional connectivity with subgenual cingulate cortex within the left dorsolateral prefrontal cortex. The Montgomery–Asberg Depression Rating Scale (MADRS) was used for clinical assessment of the effects, the follow-up period was three months. The improvement of depressive symptoms to the levels characteristic of remission immediately after the SNT completion was observed in five patients (MADRS score ≤10). After three months, two patients still had remission, the condition of three patients met the criteria of mild depressive episode, and one female patient withdrew from the study due to logistical difficulties. No serious adverse events were reported. The findings confirm safety and potentially high efficacy of SNT, including in patients with type 1 and 2 bipolar disorders.
{"title":"Experience of Stanford neuromodulation therapy in patients with treatment-resistant depression","authors":"A. Poydasheva, I. Bakulin, D. Sinitsyn, A. Zabirova, N. Suponeva, NV Maslenikov, EE Tsukarzi, S. Mosolov, M. Piradov","doi":"10.24075/brsmu.2022.044","DOIUrl":"https://doi.org/10.24075/brsmu.2022.044","url":null,"abstract":"Stanford neuromodulation therapy (SNT) is the state-of-the-art magnetic stimulation protocol that has been developed for management of treatment-resistant depression (TRD). The study was aimed to assess the possibility of SNT implementation in clinical practice and to define the protocol safety and efficacy in patients with TRD being an episode of the recurrent depressive disorder or bipolar disorder at the independent center. The study involved six patients (among them three women aged 21–66) with TRD associated with recurrent depression and type 1 or 2 bipolar disorder. The patients received intermittent theta-burst stimulation in accordance with the SNT protocol for five days: applying 10 triple blocks of stimulation daily at intervals of 1 hr between the blocks to the selected stimulation site showing maximum negative functional connectivity with subgenual cingulate cortex within the left dorsolateral prefrontal cortex. The Montgomery–Asberg Depression Rating Scale (MADRS) was used for clinical assessment of the effects, the follow-up period was three months. The improvement of depressive symptoms to the levels characteristic of remission immediately after the SNT completion was observed in five patients (MADRS score ≤10). After three months, two patients still had remission, the condition of three patients met the criteria of mild depressive episode, and one female patient withdrew from the study due to logistical difficulties. No serious adverse events were reported. The findings confirm safety and potentially high efficacy of SNT, including in patients with type 1 and 2 bipolar disorders.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47016818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JG Birulina, OV Voronkova, VV Ivanov, EE Buyko, M. Shcherbakova, NA Chernyshov, E. Motlokhova
Chronic systemic inflammation is essential in many chronic non-infectious diseases, including type 2 diabetes, obesity and metabolic syndrome (MS). This study aimed at characterization of systemic inflammatory reaction as a component of diet-induced MS in rat model. Thirty-three male Wistar rats were distributed into two groups designated 'control' (n = 15) and 'experimental (MS)' (n = 18). The groups were fed, respectively, regular and high-fat/high-carbohydrate diets for 12 weeks. The intensity of systemic inflammatory process against the background of metabolic impairments was assessed by total and differential counts of white blood cells and serum levels of total protein, C-reactive protein, cytokines (IL6, IL10 and TNFα), insulin and leptin. We also assessed the production of reactive oxygen species in adipose tissue samples. The experiment revealed signs of systemic inflammation in MS as compared to control, including reactive leukocytosis, hyperproteinemia and increased serum levels of C-reactive protein (2.6-fold; р = 0.001), IL10 (3.7-fold; р = 0.029) and TNFα (4.2-fold; р = 0.035). The observed changes were accompanied by elevated metabolic activity of visceral adipose tissue, indicated by hyperleptinemia and increased free radical oxidation intensity. Pairwise positive correlations of serum levels were revealed for leptin and insulin (r = 0.701; р = 0.001) and leptin and IL10 (r = 0.523; р = 0.012). Thus, high-fat/ high-carbohydrate diet promoted metabolic impairments concomitantly with early signs of systemic inflammation characteristic of MS and obesity.
{"title":"Systemic inflammation markers of diet-induced metabolic syndrome in rat model","authors":"JG Birulina, OV Voronkova, VV Ivanov, EE Buyko, M. Shcherbakova, NA Chernyshov, E. Motlokhova","doi":"10.24075/brsmu.2022.043","DOIUrl":"https://doi.org/10.24075/brsmu.2022.043","url":null,"abstract":"Chronic systemic inflammation is essential in many chronic non-infectious diseases, including type 2 diabetes, obesity and metabolic syndrome (MS). This study aimed at characterization of systemic inflammatory reaction as a component of diet-induced MS in rat model. Thirty-three male Wistar rats were distributed into two groups designated 'control' (n = 15) and 'experimental (MS)' (n = 18). The groups were fed, respectively, regular and high-fat/high-carbohydrate diets for 12 weeks. The intensity of systemic inflammatory process against the background of metabolic impairments was assessed by total and differential counts of white blood cells and serum levels of total protein, C-reactive protein, cytokines (IL6, IL10 and TNFα), insulin and leptin. We also assessed the production of reactive oxygen species in adipose tissue samples. The experiment revealed signs of systemic inflammation in MS as compared to control, including reactive leukocytosis, hyperproteinemia and increased serum levels of C-reactive protein (2.6-fold; р = 0.001), IL10 (3.7-fold; р = 0.029) and TNFα (4.2-fold; р = 0.035). The observed changes were accompanied by elevated metabolic activity of visceral adipose tissue, indicated by hyperleptinemia and increased free radical oxidation intensity. Pairwise positive correlations of serum levels were revealed for leptin and insulin (r = 0.701; р = 0.001) and leptin and IL10 (r = 0.523; р = 0.012). Thus, high-fat/ high-carbohydrate diet promoted metabolic impairments concomitantly with early signs of systemic inflammation characteristic of MS and obesity.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46984629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Vagner, EA Sarf, L. Belskaya, A. Korshunov, KN Kuryatnikov, AA Bondar, A. Meloyan, K. Maksimenko, M. Kasiy
Oral fluid is a valuable substrate for assessing dental health and other aspects of physical status. New methods for early diagnosis and prognosis of dental diseases on the basis of oral fluid composition are in constant demand. Excessive fluoride concentrations, often oversighted by dental therapists, negatively affect organs and tissues of the oral cavity. This study aimed at development and approbation of a method for reliable measurement of fluoride ions in oral fluid by capillary electrophoresis to be used in patients with dental diseases. The fluoride ion concentrations were measured in health (2.16 ± 0.48 mg/L), in isolated acute pericoronitis (15.2 ± 2.7 mg/L) and in acute pericoronitis combined to multiple caries (18.9 ± 4.2 mg/L). By post-operative day 3, fluoride levels in the group with isolated acute pericoronitis dropped to normal values (2.28 ± 0.52 mg/L), whereas in the group with acute pericoronitis combined to multiple caries fluoride levels remained high (8.7 ± 1.9 mg/L; р < 0.0001). The developed protocol is efficient for studying fluoride ion concentrations in isolated and combined dental diseases.
{"title":"Prognostic significance of oral fluid fluoride measurement in acute pericoronitis","authors":"V. Vagner, EA Sarf, L. Belskaya, A. Korshunov, KN Kuryatnikov, AA Bondar, A. Meloyan, K. Maksimenko, M. Kasiy","doi":"10.24075/brsmu.2022.042","DOIUrl":"https://doi.org/10.24075/brsmu.2022.042","url":null,"abstract":"Oral fluid is a valuable substrate for assessing dental health and other aspects of physical status. New methods for early diagnosis and prognosis of dental diseases on the basis of oral fluid composition are in constant demand. Excessive fluoride concentrations, often oversighted by dental therapists, negatively affect organs and tissues of the oral cavity. This study aimed at development and approbation of a method for reliable measurement of fluoride ions in oral fluid by capillary electrophoresis to be used in patients with dental diseases. The fluoride ion concentrations were measured in health (2.16 ± 0.48 mg/L), in isolated acute pericoronitis (15.2 ± 2.7 mg/L) and in acute pericoronitis combined to multiple caries (18.9 ± 4.2 mg/L). By post-operative day 3, fluoride levels in the group with isolated acute pericoronitis dropped to normal values (2.28 ± 0.52 mg/L), whereas in the group with acute pericoronitis combined to multiple caries fluoride levels remained high (8.7 ± 1.9 mg/L; р < 0.0001). The developed protocol is efficient for studying fluoride ion concentrations in isolated and combined dental diseases.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47456058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
VO Soldatov, M. Pokrovskiy, O. Puchenkova, NS Zhunusov, A. Krayushkina, AV Grechina, M. Soldatova, K. Lapin, OYu Bushueva
Mitochondrial dysfunction is a key driver of neurodegeneration. This study aimed to evaluate the protective potential of EPOR/CD131 (heterodimeric erythropoietin receptor) stimulation in the neurodegeneration caused by rotenone-induced mitochondrial dysfunction. The effects of erythropoietin (EPO) and an EPO mimetic peptide pHBSP were assessed using in vivo and in vitro models. Single injections of 10 µg/kg EPO or 5 µg/kg pHBSP significantly alleviated the degeneration of ganglion cells of the retina in a rotenone-induced retinopathy in rats (p < 0.05). Consistently, in vitro exposure of rotenone-treated murine primary neuroglial cultures to 500 nM EPO or pHBSP significantly rescued the survival of the cells (p < 0.005). The observed enhancement of LC3A, ATG7, Beclin-1, Parkin and BNIP3 mRNA expression by EPOR/CD131 agonists implicates the autophagy and mitophagy activation as a plausible mitoprotective mechanism.
{"title":"EPOR/CD131-mediated attenuation of rotenone-induced retinal degeneration is associated with upregulation of autophagy genes","authors":"VO Soldatov, M. Pokrovskiy, O. Puchenkova, NS Zhunusov, A. Krayushkina, AV Grechina, M. Soldatova, K. Lapin, OYu Bushueva","doi":"10.24075/brsmu.2022.040","DOIUrl":"https://doi.org/10.24075/brsmu.2022.040","url":null,"abstract":"Mitochondrial dysfunction is a key driver of neurodegeneration. This study aimed to evaluate the protective potential of EPOR/CD131 (heterodimeric erythropoietin receptor) stimulation in the neurodegeneration caused by rotenone-induced mitochondrial dysfunction. The effects of erythropoietin (EPO) and an EPO mimetic peptide pHBSP were assessed using in vivo and in vitro models. Single injections of 10 µg/kg EPO or 5 µg/kg pHBSP significantly alleviated the degeneration of ganglion cells of the retina in a rotenone-induced retinopathy in rats (p < 0.05). Consistently, in vitro exposure of rotenone-treated murine primary neuroglial cultures to 500 nM EPO or pHBSP significantly rescued the survival of the cells (p < 0.005). The observed enhancement of LC3A, ATG7, Beclin-1, Parkin and BNIP3 mRNA expression by EPOR/CD131 agonists implicates the autophagy and mitophagy activation as a plausible mitoprotective mechanism.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49153516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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