Pub Date : 2022-09-29DOI: 10.56825/bufbu.2022.4134917
Manasi Soni, J. B. Nayak, Pranav Anjaria, Prakrutik Bhavsar, Jeetendrakumar H. Chaudhary, M. Brahmbhatt
The present study was conducted to determine the drug resistance pattern and the occurrence of biofilm producing Enterococcus faecalis from buffalo meat samples collected from in and around the Anand city, Gujarat. A total of 100 samples (meat, hand’s swab, knife’s swab) were collected aseptically from the butcher shops. Out of 100 samples, 52 (52%) samples were found to be positive on selective media, which were subjected to polymerase chain reaction revealing that 40 isolates were of Enterococcus faecalis. Antibiotic sensitivity test showed that all the isolates were sensitive to Ampicillin (100%); sensitive to intermediate for Gentamicin and resistance was observed against Tigecycline (85%), Trimethoprim (60%), Vancomycin (50%), Norfloxacin (37.5%), and Imipenem-cilastatin (25%). Out of all the PCR positive isolates, 95% (38/40) were biofilm producers when observed phenotypically on Congo Red Agar (CRA). So, it can be concluded that the buffalo meat can be a possible intermediary vehicle for the spread of multidrug-resistant biofilm producing enterococci strains to humans.
{"title":"Isolation and molecular detection of biofilm producing and multiple drug resistant Enterococcus faecalis from the buffalo meat","authors":"Manasi Soni, J. B. Nayak, Pranav Anjaria, Prakrutik Bhavsar, Jeetendrakumar H. Chaudhary, M. Brahmbhatt","doi":"10.56825/bufbu.2022.4134917","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4134917","url":null,"abstract":"The present study was conducted to determine the drug resistance pattern and the occurrence of biofilm producing Enterococcus faecalis from buffalo meat samples collected from in and around the Anand city, Gujarat. A total of 100 samples (meat, hand’s swab, knife’s swab) were collected aseptically from the butcher shops. Out of 100 samples, 52 (52%) samples were found to be positive on selective media, which were subjected to polymerase chain reaction revealing that 40 isolates were of Enterococcus faecalis. Antibiotic sensitivity test showed that all the isolates were sensitive to Ampicillin (100%); sensitive to intermediate for Gentamicin and resistance was observed against Tigecycline (85%), Trimethoprim (60%), Vancomycin (50%), Norfloxacin (37.5%), and Imipenem-cilastatin (25%). Out of all the PCR positive isolates, 95% (38/40) were biofilm producers when observed phenotypically on Congo Red Agar (CRA). So, it can be concluded that the buffalo meat can be a possible intermediary vehicle for the spread of multidrug-resistant biofilm producing enterococci strains to humans. \u0000 ","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42136589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-29DOI: 10.56825/bufbu.2022.4133165
Prajakta Kuralkar, S. Kuralkar
The present study was designed to observe the effect of season and stage of lactation on milk components of Purnathadi buffaloes. Total 346 milk samples were collected, from three different seasons (winter, summer and rainy) and stage of lactation (early, mid and late) for the study. Overall milk fat, protein, lactose, solid not fat (SNF) and total solids (TS) were observed as 8.44±0.07, 3.99±0.02, 3.96±0.02, 8.78±0.04, and 17.23±0.08% respectively. There was significantly higher fat and total solids percent during winter (9.01±0.23 and 17.62±0.26% respectively) and lower during summer (8.25±0.14 and 16.73±0.14% respectively) season, but milk SNF percent was significantly higher during rainy (9.00±0.04%) and lower during summer (8.48±0.05%). Stage of lactation had also significant effect on milk components; milk fat, protein, SNF, and TS percent increased significantly with the advancement of lactation stage, whereas milk lactose was nonsignificant. Milk fat during early, mid and late lactation was 7.43±0.10, 8.40±0.07 and 8.79±0.12%; protein was 3.83±0.06, 3.94±0.02 and 4.06±0.02%; SNF was 8.36±0.14, 8.70±0.04 and 8.96±0.05% and TS was 15.61±0.17, 17.10±0.08 and 17.75±0.12%, respectively. Thus, results of the present investigation indicated that season and stage of lactation affect certain milk components in Purnathadi buffaloes and could be minimized by better farm management practices.
{"title":"Effect of season and stage of lactation on milk components in Purnathadi buffaloes","authors":"Prajakta Kuralkar, S. Kuralkar","doi":"10.56825/bufbu.2022.4133165","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4133165","url":null,"abstract":"The present study was designed to observe the effect of season and stage of lactation on milk components of Purnathadi buffaloes. Total 346 milk samples were collected, from three different seasons (winter, summer and rainy) and stage of lactation (early, mid and late) for the study. Overall milk fat, protein, lactose, solid not fat (SNF) and total solids (TS) were observed as 8.44±0.07, 3.99±0.02, 3.96±0.02, 8.78±0.04, and 17.23±0.08% respectively. There was significantly higher fat and total solids percent during winter (9.01±0.23 and 17.62±0.26% respectively) and lower during summer (8.25±0.14 and 16.73±0.14% respectively) season, but milk SNF percent was significantly higher during rainy (9.00±0.04%) and lower during summer (8.48±0.05%). Stage of lactation had also significant effect on milk components; milk fat, protein, SNF, and TS percent increased significantly with the advancement of lactation stage, whereas milk lactose was nonsignificant. Milk fat during early, mid and late lactation was 7.43±0.10, 8.40±0.07 and 8.79±0.12%; protein was 3.83±0.06, 3.94±0.02 and 4.06±0.02%; SNF was 8.36±0.14, 8.70±0.04 and 8.96±0.05% and TS was 15.61±0.17, 17.10±0.08 and 17.75±0.12%, respectively. Thus, results of the present investigation indicated that season and stage of lactation affect certain milk components in Purnathadi buffaloes and could be minimized by better farm management practices.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45144136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.56825/bufbu.2022.4132403
A. Tripathi, A. Srivastava, Padmnibash Panigrahi, M. Srivastava, Ram Sagar
The study was carried out on 36 naturally infected buffaloes with Theileria annulata, to determine the clinical markers, hemato-biochemical and oxidative stress parameters alterations. The clinical markers recorded in infected buffaloes were fever, enlargement of pre-scapular lymph nodes, pale mucosae, anorexia, thin mucoid nasal discharge along with melanic faeces and corneal opacity as clinical signs which might be considered as clinical markers of theleriosis in buffaloes in the semi arid zone of the region. Hemato-bichemical and oxidative stress parameters alterations revealed significant decrease in red blood cells (RBC) and haemoglobin (Hb) concentration in the infected animals, compared to the control ones. Increased lipid peroxidation (LPO) and decrease in the levels superoxide dismutase (SOD) and catalase (CAT) in infected animals, compared to the control ones. It can be concluded that theleriosis in buffaloes may lead to anemia which might be attributed due to increased oxidative stress.
{"title":"Studdies on clinical markers, hemato-biochemical and oxidative stress parameter alterations in theileriosis affected buffaloes from semi arid zone of northern plains of India","authors":"A. Tripathi, A. Srivastava, Padmnibash Panigrahi, M. Srivastava, Ram Sagar","doi":"10.56825/bufbu.2022.4132403","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4132403","url":null,"abstract":"The study was carried out on 36 naturally infected buffaloes with Theileria annulata, to determine the clinical markers, hemato-biochemical and oxidative stress parameters alterations. The clinical markers recorded in infected buffaloes were fever, enlargement of pre-scapular lymph nodes, pale mucosae, anorexia, thin mucoid nasal discharge along with melanic faeces and corneal opacity as clinical signs which might be considered as clinical markers of theleriosis in buffaloes in the semi arid zone of the region. Hemato-bichemical and oxidative stress parameters alterations revealed significant decrease in red blood cells (RBC) and haemoglobin (Hb) concentration in the infected animals, compared to the control ones. Increased lipid peroxidation (LPO) and decrease in the levels superoxide dismutase (SOD) and catalase (CAT) in infected animals, compared to the control ones. It can be concluded that theleriosis in buffaloes may lead to anemia which might be attributed due to increased oxidative stress.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48943470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.56825/bufbu.2022.4134205
A. Amin, M. Khalil, K. Mourad, M. K. Ibrahim, E. Afifi
The genetic gains were estimated for milk production and persistency, derived from random regression models, using eigenvector indices, and they were compared with the traditional selection index. The data set contained 4971 test day milk yield recorded for 691 buffalo cows, daughters of 120 sires and 532 dams. The model included the random effects of direct additive genetic, permanent environment and error, whereas the fixed effects were herd test day, year and season of calving and parity, and as a covariate, it was milk days. The first and the 2nd eigenvalues explained 73.1 and 22.9% of the variation of the random regression coefficients, respectively, suggesting that the use of the first two eigenvectors is sufficient. Genetic responses in total milk yield (TMY) based on the first eigenvector index (Ie1) and that based on the conventional selection (IMY) have close gain of about 171 kg in each index. The second eigenvector index (Ie2) showed an increase in TMY (9.91 kg), and thus an increase in the persistency (0.86 kg). The TMY and persistency are the two economically important traits in dairy production, additional genetic gains in persistency and high genetic gain for TMY could be obtained using the 2nd eigenvector index (I*2).
{"title":"Eigenvector selection indices for improving milk yield and persistency in Egyptian buffalo","authors":"A. Amin, M. Khalil, K. Mourad, M. K. Ibrahim, E. Afifi","doi":"10.56825/bufbu.2022.4134205","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4134205","url":null,"abstract":"The genetic gains were estimated for milk production and persistency, derived from random regression models, using eigenvector indices, and they were compared with the traditional selection index. The data set contained 4971 test day milk yield recorded for 691 buffalo cows, daughters of 120 sires and 532 dams. The model included the random effects of direct additive genetic, permanent environment and error, whereas the fixed effects were herd test day, year and season of calving and parity, and as a covariate, it was milk days. The first and the 2nd eigenvalues explained 73.1 and 22.9% of the variation of the random regression coefficients, respectively, suggesting that the use of the first two eigenvectors is sufficient. Genetic responses in total milk yield (TMY) based on the first eigenvector index (Ie1) and that based on the conventional selection (IMY) have close gain of about 171 kg in each index. The second eigenvector index (Ie2) showed an increase in TMY (9.91 kg), and thus an increase in the persistency (0.86 kg). The TMY and persistency are the two economically important traits in dairy production, additional genetic gains in persistency and high genetic gain for TMY could be obtained using the 2nd eigenvector index (I*2).","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46681803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.56825/bufbu.2022.4134795
Annadurai Rajadurai, S. Alimudeen, Natarajan Kumaravelu
A study was conducted to identify the constraints perceived by the buffalo farmers in Kallakurichi district of Tamil Nadu. For this study, data were collected from the randomly selected buffalo farmers by using a structured interview schedule. The present study indicated that the major constraints faced by buffalo dairy farmers were high cost of concentrates (63.82% Garrett score), followed by high capital investment in animal housing (53.64% Garrett score), Low conception rate (58.92% Garrett score) repeat breeding (60.20% Garrett score), lower milk price 64.34% Garrett score), Unavailability of credit facilities from government side (50.80% Garrett score), high cost involved in veterinary care (56.28% Garrett score) and reproductive disorders (48.70% Garrett score).
{"title":"Constraints of buffalo farming in Kallakurichi district of Tamil Nadu, India","authors":"Annadurai Rajadurai, S. Alimudeen, Natarajan Kumaravelu","doi":"10.56825/bufbu.2022.4134795","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4134795","url":null,"abstract":"A study was conducted to identify the constraints perceived by the buffalo farmers in Kallakurichi district of Tamil Nadu. For this study, data were collected from the randomly selected buffalo farmers by using a structured interview schedule. The present study indicated that the major constraints faced by buffalo dairy farmers were high cost of concentrates (63.82% Garrett score), followed by high capital investment in animal housing (53.64% Garrett score), Low conception rate (58.92% Garrett score) repeat breeding (60.20% Garrett score), lower milk price 64.34% Garrett score), Unavailability of credit facilities from government side (50.80% Garrett score), high cost involved in veterinary care (56.28% Garrett score) and reproductive disorders (48.70% Garrett score).","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41921721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.56825/bufbu.2022.4134281
A. ., H. Mehta, Madhu Shivhare, Rahul Chourasia
The present study was carried out at Veterinary Clinical Complex (V.C.C.), College of Veterinary Science and Animal Husbandry, Mhow. The present investigation was undertaken to study the Epidemiology of Haemoprotozoan Diseases in Buffaloes using Giemsa’s staining technique and also to correlate the meteorological parameter with the incidence of haemoprotozoan diseases. The samples were collected from the randomly selected buffaloes belonging to three districts viz Indore, Dhar and Alirajpur of Madhya Pradesh. A total of 756 samples were collected from different buffaloes in which 252 samples were collected from each selected district. The incidence of haemoprotozoan diseases was recorded in three districts as 58.32% in Indore district followed by 29.75% and 28.56% in Alirajpur and Dhar district respectively. The highest prevalence was found in adult’s age, followed by in heifers and lowest in calves in all three districts.
{"title":"Incidence of various haemoprotozoan diseases in buffaloes","authors":"A. ., H. Mehta, Madhu Shivhare, Rahul Chourasia","doi":"10.56825/bufbu.2022.4134281","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4134281","url":null,"abstract":"The present study was carried out at Veterinary Clinical Complex (V.C.C.), College of Veterinary Science and Animal Husbandry, Mhow. The present investigation was undertaken to study the Epidemiology of Haemoprotozoan Diseases in Buffaloes using Giemsa’s staining technique and also to correlate the meteorological parameter with the incidence of haemoprotozoan diseases. The samples were collected from the randomly selected buffaloes belonging to three districts viz Indore, Dhar and Alirajpur of Madhya Pradesh. A total of 756 samples were collected from different buffaloes in which 252 samples were collected from each selected district. The incidence of haemoprotozoan diseases was recorded in three districts as 58.32% in Indore district followed by 29.75% and 28.56% in Alirajpur and Dhar district respectively. The highest prevalence was found in adult’s age, followed by in heifers and lowest in calves in all three districts.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46913150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.56825/bufbu.2022.4134101
L. Singh, Chaju Ram Chaudhary, U. Singh, Prem Singh
The successful delivery of a rare case of dicephalic fetal monster using forceful traction in a Murrah buffalo is reported.
成功交付的一个罕见的情况下，双头畸形胎儿怪物使用强力牵引Murrah水牛报道。
{"title":"Dicephalus fetal monster in a Murrah buffalo: A case report","authors":"L. Singh, Chaju Ram Chaudhary, U. Singh, Prem Singh","doi":"10.56825/bufbu.2022.4134101","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4134101","url":null,"abstract":"The successful delivery of a rare case of dicephalic fetal monster using forceful traction in a Murrah buffalo is reported.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48116962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neonatal ruminants are born without any humoral immunity due to lack of placental transfer of immunoglobulins during gestation. This predisposes the newborn buffalo calves to a high incidence of morbidity and mortality on exposure to infectious agents. Colostrum is the first milk produced by the females after calving and is a rich source of immunoglobulins, especially immunoglobulin G (IgG). The immunocompetence of the neonates can be boosted by feeding them sufficient amount of good quality colostrum within a few hours after birth. Optimal colostrum management at a farm not only reduces the occurrence of diseases among the younger stock but also enhances their growth performance and productivity once they are adults. In the present study, eighty animals at a Murrah buffalo farm were screened for the concentration of IgG in the colostrum collected from recently parturated females and in the serum collected from their calves within 6 to 12 h of colostrum consumption to determine the status of transfer of passive immunity. Indirect ELISA was used to estimate the IgG levels. The overall mean (range) of colostral and serum IgG concentration was found to be 50.44±3.36 (12.71 to 227.78) and 10.85±0.62 (0.25 to 19.88) mg/ml, respectively for all the 80 animals. Routine screening of buffaloes, in a similar way, will help to reduce calf deaths due to immunodeficiency.
{"title":"Assaying the concentration of immunoglobulin G in colostrum from females postpartum and serum from neonatal calves of buffaloes (Bubalus bubalis)","authors":"Swati Agrawal, T. Kumar, Rajni Chaudhary, Anitta Pulikan Lionel, Subodh Kumar","doi":"10.56825/bufbu.2022.4134858","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4134858","url":null,"abstract":"Neonatal ruminants are born without any humoral immunity due to lack of placental transfer of immunoglobulins during gestation. This predisposes the newborn buffalo calves to a high incidence of morbidity and mortality on exposure to infectious agents. Colostrum is the first milk produced by the females after calving and is a rich source of immunoglobulins, especially immunoglobulin G (IgG). The immunocompetence of the neonates can be boosted by feeding them sufficient amount of good quality colostrum within a few hours after birth. Optimal colostrum management at a farm not only reduces the occurrence of diseases among the younger stock but also enhances their growth performance and productivity once they are adults. In the present study, eighty animals at a Murrah buffalo farm were screened for the concentration of IgG in the colostrum collected from recently parturated females and in the serum collected from their calves within 6 to 12 h of colostrum consumption to determine the status of transfer of passive immunity. Indirect ELISA was used to estimate the IgG levels. The overall mean (range) of colostral and serum IgG concentration was found to be 50.44±3.36 (12.71 to 227.78) and 10.85±0.62 (0.25 to 19.88) mg/ml, respectively for all the 80 animals. Routine screening of buffaloes, in a similar way, will help to reduce calf deaths due to immunodeficiency.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49034559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.56825/bufbu.2022.4131234
B. K. Pramanik, S. Batabyal, A. Maity, S. De, S. Chattopadhyay, A. Barui
The caseins (Alpha s1, Alpha s2, beta and kappa) comprise the major protein component of ruminant milk and are secreted in the form of stable calcium phosphate micelles. The present study was proposed to characterize αs1-casein gene in buffalo at the molecular level to determine complete αs1-casein cDNA sequence. Sequencing results were analyzed and aligned with available mRNA GenBank sequences of other domestic animals (Accession AAB34797, NM_001009795, X72221, AY344966, NM_181029, AY948385 and BC109618). The complete amino acid coding region of the buffalo αs1- casein was deduced from cloned cDNA generated from mammary tissue mRNA. Amino acid sequence comprises of 199 amino acid residues. When aligned with previously reported buffalo sequence (Accession-AY948385) it showed one amino acid (Glutamine) deletion at amino acid position 78.
{"title":"Molecular characterization of buffalo αs1-casein gene","authors":"B. K. Pramanik, S. Batabyal, A. Maity, S. De, S. Chattopadhyay, A. Barui","doi":"10.56825/bufbu.2022.4131234","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4131234","url":null,"abstract":"The caseins (Alpha s1, Alpha s2, beta and kappa) comprise the major protein component of ruminant milk and are secreted in the form of stable calcium phosphate micelles. The present study was proposed to characterize αs1-casein gene in buffalo at the molecular level to determine complete αs1-casein cDNA sequence. Sequencing results were analyzed and aligned with available mRNA GenBank sequences of other domestic animals (Accession AAB34797, NM_001009795, X72221, AY344966, NM_181029, AY948385 and BC109618). The complete amino acid coding region of the buffalo αs1- casein was deduced from cloned cDNA generated from mammary tissue mRNA. Amino acid sequence comprises of 199 amino acid residues. When aligned with previously reported buffalo sequence (Accession-AY948385) it showed one amino acid (Glutamine) deletion at amino acid position 78.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"181 3","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41259089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was conducted on four Jaffarabadi breeding bulls, 5 to 6 years old, to know the neat characteristics based on total of 192 semen ejaculates evaluated and cryopreserved during winter (10 to 25°C mean temperature) of November to February (n=96 ejaculates) and summer (38 to 45°C temperature) March to June (n=96 ejaculates). The neat seminal characteristics of Jaffarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOST reactive sperm (%) and acrosomal integrity (%), first AI conception rate observed during winter season were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43, 93.56±0.56, 45.75±0.21 respectively, whereas the respective values in summer were 5.07±0.13, 2.00±0.00, 1186.60±24.78, 3.72±0.11, 78.75±0.46, 82.31±0.74, 7.13±0.52, 84.93±0.45, 90.56±0.23, 43.50±0.11. The semen quality of fresh samples was observed better during winter compared to summer season for use in breeding program with satisfactory first AI conception rate.
{"title":"Seasonal variation in semen quality and conception rate of Jaffarabadi buffalo bulls (Bubalus bubalis) in India","authors":"Parmar Kirankumar Hirabhai, Tank Prafulchandra Hirjibhai, Savsani Harishkumar Hirjibhai, Vala Karsanbhai Bhagvanbhai","doi":"10.56825/bufbu.2022.4133642","DOIUrl":"https://doi.org/10.56825/bufbu.2022.4133642","url":null,"abstract":"The study was conducted on four Jaffarabadi breeding bulls, 5 to 6 years old, to know the neat characteristics based on total of 192 semen ejaculates evaluated and cryopreserved during winter (10 to 25°C mean temperature) of November to February (n=96 ejaculates) and summer (38 to 45°C temperature) March to June (n=96 ejaculates). The neat seminal characteristics of Jaffarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOST reactive sperm (%) and acrosomal integrity (%), first AI conception rate observed during winter season were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43, 93.56±0.56, 45.75±0.21 respectively, whereas the respective values in summer were 5.07±0.13, 2.00±0.00, 1186.60±24.78, 3.72±0.11, 78.75±0.46, 82.31±0.74, 7.13±0.52, 84.93±0.45, 90.56±0.23, 43.50±0.11. The semen quality of fresh samples was observed better during winter compared to summer season for use in breeding program with satisfactory first AI conception rate.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47804912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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