Pub Date : 2023-09-29DOI: 10.56825/bufbu.2023.4233089
Amar Nasir, Arbab Sikandar, Abdul Shakoor, Muhammad Kashif, Rana Waqar Tabish, Asghar Hussain, Adnan Hassan Tahir, Mazhar Abbas, Muhammad Umer Iqbal
Bovine ketosis is a major metabolic disorder emerging as a result of negative energy balance in the post calving period and subsequent lactation of lactating dairy animals. The ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone etc.) are accumulated that can be detected in milk, blood, and urine samples. The present study was accomplished to sort out the frequency of bovine ketosis in dairy buffalo herds during the first 2 months of lactation in buffalo to detect bovine ketosis. For this purpose, blood, and urine samples (n=50) were randomly collected from private dairy farms (n=5) having buffalos as a pilot study employing commercial test strips i.e., Free Style Optium β-ketone test strips (Abbot Healthcare, Pvt. Ltd. UK) for blood and JusChek (Muenster, Germany) for urine analysis. Resultantly, as a whole prevalence of bovine ketosis found using blood sample was 18% and via urine was 12% that indicates high prevalence of subclinical ketosis during 2nd month of lactation. Animals in the age group of 5 to 7 years were found to be significantly more susceptible (P<0.05) with an incidence of 37.5%. The results revealed that a significant subclinical ketosis exists in the dairy buffaloes, which causes decreased production associated with significant economic loss to the farmers. It was concluded that a moderately high incidence of sub clinical bovine ketosis predominates in the study area which may be curtailed by early diagnostic and remedial approaches using field tests to save the economics of the farmers.
{"title":"Detection of subclinical ketosis in dairy buffalo herds of Tehsil Jhang, Punjab, Pakistan","authors":"Amar Nasir, Arbab Sikandar, Abdul Shakoor, Muhammad Kashif, Rana Waqar Tabish, Asghar Hussain, Adnan Hassan Tahir, Mazhar Abbas, Muhammad Umer Iqbal","doi":"10.56825/bufbu.2023.4233089","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233089","url":null,"abstract":"Bovine ketosis is a major metabolic disorder emerging as a result of negative energy balance in the post calving period and subsequent lactation of lactating dairy animals. The ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone etc.) are accumulated that can be detected in milk, blood, and urine samples. The present study was accomplished to sort out the frequency of bovine ketosis in dairy buffalo herds during the first 2 months of lactation in buffalo to detect bovine ketosis. For this purpose, blood, and urine samples (n=50) were randomly collected from private dairy farms (n=5) having buffalos as a pilot study employing commercial test strips i.e., Free Style Optium β-ketone test strips (Abbot Healthcare, Pvt. Ltd. UK) for blood and JusChek (Muenster, Germany) for urine analysis. Resultantly, as a whole prevalence of bovine ketosis found using blood sample was 18% and via urine was 12% that indicates high prevalence of subclinical ketosis during 2nd month of lactation. Animals in the age group of 5 to 7 years were found to be significantly more susceptible (P<0.05) with an incidence of 37.5%. The results revealed that a significant subclinical ketosis exists in the dairy buffaloes, which causes decreased production associated with significant economic loss to the farmers. It was concluded that a moderately high incidence of sub clinical bovine ketosis predominates in the study area which may be curtailed by early diagnostic and remedial approaches using field tests to save the economics of the farmers.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135296060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-29DOI: 10.56825/bufbu.2023.4233521
Balbir Singh Khadda, Kanak Lata, Raj Kumar
Pashu chocolate is the brand name of the urea molasses mineral block formulated by KVK, Panchmahal by using of urea, molasses, wheat bran, area specific mineral mixture, black salt, etc. for augment milk production and reproductive performance of dairy animals. A study was conducted to find out the efficacy of Pashu chocolate supplementation on productive and reproductive performance of milking buffalo and feed economics at farmers field in semi-arid zone, during the year 2018 to 2019. Twenty, lactating Mehsana breed of buffalo were randomly selected and distributed equally in to two groups ten each in a completely randomized design i.e., T1 (Control) and T2 (Pashu chocolate supplementations). In the feeding schedule of the present study T1 animals were given 5 to 6 kg dry roughage (sorghum/pearl millet) with green fodder (20 kg Napier-lucerne) added to 2.5 kg concentrate feed/ day, whereas an ad lib lick of UMMB (Pashu chocolate) was offered in the T2 Group in addition to the feeding schedule of T1 Group. An increase of 24.56% in milk i.e., from 6.23±0.40 to 7.76±0.54 L per day was recorded after comparing the outcomes of the two treatments. This significant (P˂0.05) increase in milk production indicates a positive impact of Pashu chocolate on the performance of milch buffaloes. Supplementation of Pashu chocolate as an ad lib lick reduced the postpartum estrus period and service period from 79.7±7.43 and 132.00±13.34 days to 54.3±4.41 and 81.60±7.54 days, respectively. Hence, the findings in the present study indicate beneficial effects of Pashu chocolate on productive and reproductive traits of buffaloes in semi-arid climatic conditions.
{"title":"Effect of Pashu chocolate supplementation on production and reproductive performance of milch buffalo under field conditions of semi arid region","authors":"Balbir Singh Khadda, Kanak Lata, Raj Kumar","doi":"10.56825/bufbu.2023.4233521","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233521","url":null,"abstract":"Pashu chocolate is the brand name of the urea molasses mineral block formulated by KVK, Panchmahal by using of urea, molasses, wheat bran, area specific mineral mixture, black salt, etc. for augment milk production and reproductive performance of dairy animals. A study was conducted to find out the efficacy of Pashu chocolate supplementation on productive and reproductive performance of milking buffalo and feed economics at farmers field in semi-arid zone, during the year 2018 to 2019. Twenty, lactating Mehsana breed of buffalo were randomly selected and distributed equally in to two groups ten each in a completely randomized design i.e., T1 (Control) and T2 (Pashu chocolate supplementations). In the feeding schedule of the present study T1 animals were given 5 to 6 kg dry roughage (sorghum/pearl millet) with green fodder (20 kg Napier-lucerne) added to 2.5 kg concentrate feed/ day, whereas an ad lib lick of UMMB (Pashu chocolate) was offered in the T2 Group in addition to the feeding schedule of T1 Group. An increase of 24.56% in milk i.e., from 6.23±0.40 to 7.76±0.54 L per day was recorded after comparing the outcomes of the two treatments. This significant (P˂0.05) increase in milk production indicates a positive impact of Pashu chocolate on the performance of milch buffaloes. Supplementation of Pashu chocolate as an ad lib lick reduced the postpartum estrus period and service period from 79.7±7.43 and 132.00±13.34 days to 54.3±4.41 and 81.60±7.54 days, respectively. Hence, the findings in the present study indicate beneficial effects of Pashu chocolate on productive and reproductive traits of buffaloes in semi-arid climatic conditions.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-29DOI: 10.56825/bufbu.2023.4232682
Nunna Veera Venkata Hari Krishna
A primiparous non-descript buffalo heifer in ninth month of gestation was presented for showing the signs of abortion, i.e., vaginal discharges and rupture of water bag (choreo-allantois). Obstetrical examination revealed an open cervix. The foetus was in anterior longitudinal presentation, dorso-sacral position and was locked in pelvic cavity. A round sructure was observed in the pelvic cavity. Based on history and clinical observations, it was diagnosed as late gestational threatened abortion. It was decided to deliver the calf by manual traction, if not possible, by cesarean section. Manual traction on the foetus using snares was applied and a dead foetus with hydrocephalus was delivered per vagina.
{"title":"Dystocia due to external hydrocephalus in a primiparous buffalo heifer","authors":"Nunna Veera Venkata Hari Krishna","doi":"10.56825/bufbu.2023.4232682","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4232682","url":null,"abstract":"A primiparous non-descript buffalo heifer in ninth month of gestation was presented for showing the signs of abortion, i.e., vaginal discharges and rupture of water bag (choreo-allantois). Obstetrical examination revealed an open cervix. The foetus was in anterior longitudinal presentation, dorso-sacral position and was locked in pelvic cavity. A round sructure was observed in the pelvic cavity. Based on history and clinical observations, it was diagnosed as late gestational threatened abortion. It was decided to deliver the calf by manual traction, if not possible, by cesarean section. Manual traction on the foetus using snares was applied and a dead foetus with hydrocephalus was delivered per vagina.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4225257
H. Guler, G. Yarim, M. Yarım, O. Duru, Ayris Gokceoglu
The aim of this study was to investigate the relationship between milk insulin-like growth factor-2 (IGF-2) concentration and milk yield in Anatolian water buffaloes. This study was conducted on milk samples from 80 Anatolian water buffaloes. Milk samples collected from buffaloes were evaluated for subclinical mastitis. For this purpose, the California mastitis test and somatic cell count were performed in milk samples taken from four different mammary lobes of buffaloes. The milk IGF-2 level was determined by an enzyme-linked immunosorbent assay kit. Milk IGF-2 concentration ranged from 21.6 ng/ml - 63.2 ng/ml in Anatolian water buffaloes. The IGF-2 concentration in Anatolian water buffaloes milk was 40.1±8.5 ng/ml. Milk IGF-2 was positively correlated with milk yield (r2=0.941, P<0.01). Our results showed that milk IGF-2 concentration was associated with milk yield in Anatolian buffaloes. These findings show that locally synthesized IGF-2 can affect milk yield. This study contributes to the understanding of the composition of buffalo milk, which has an important value in human nutrition. It is recommended to confirm the results of similar measurements in milk from other animal species used for human consumption.
{"title":"The milk IGF-2 level is positively correlated with milk yield in Anatolian water buffaloes","authors":"H. Guler, G. Yarim, M. Yarım, O. Duru, Ayris Gokceoglu","doi":"10.56825/bufbu.2023.4225257","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4225257","url":null,"abstract":"The aim of this study was to investigate the relationship between milk insulin-like growth factor-2 (IGF-2) concentration and milk yield in Anatolian water buffaloes. This study was conducted on milk samples from 80 Anatolian water buffaloes. Milk samples collected from buffaloes were evaluated for subclinical mastitis. For this purpose, the California mastitis test and somatic cell count were performed in milk samples taken from four different mammary lobes of buffaloes. The milk IGF-2 level was determined by an enzyme-linked immunosorbent assay kit. Milk IGF-2 concentration ranged from 21.6 ng/ml - 63.2 ng/ml in Anatolian water buffaloes. The IGF-2 concentration in Anatolian water buffaloes milk was 40.1±8.5 ng/ml. Milk IGF-2 was positively correlated with milk yield (r2=0.941, P<0.01). Our results showed that milk IGF-2 concentration was associated with milk yield in Anatolian buffaloes. These findings show that locally synthesized IGF-2 can affect milk yield. This study contributes to the understanding of the composition of buffalo milk, which has an important value in human nutrition. It is recommended to confirm the results of similar measurements in milk from other animal species used for human consumption.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49292810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A retrospective study was conducted in a total of 89 water buffaloes reported with cervico-vaginal prolapse (CVP) in various degrees and were initially examined for their physiological and haematological parameters. The affected buffaloes were classified into 3 groups based on the degree of CVP and were treated with 8 'R' principles and as a recurrence prevention strategy, they were managed with modified conventional vulval truss method, vulval retention suture method and both in all the three groups. The buffaloes handled with modified conventional vulval truss method showed reduced straining, genital injury and no obstetrical complications. The buffaloes treated with vulval retention suture showed mild straining, wound around the sutured site and those buffaloes treated with both modified conventional vulval truss method and vulval retention suture showed relatively effective results. Based on this study, it is concluded that the buffaloes affected with any degree of CVP and treated with modified conventional vulval truss method showed effective recurrence prevention with relatively no swelling, straining, haemorrhage, injury, maggot wound and postpartum obstetrical complications.
{"title":"Modified vulval truss technique to prevent recurrence of cervico-vaginal prolapse in water buffaloes","authors":"Maniprabhu Selvaraju, Subramanian Prakash, V. Varudharajan, Mani Periyannan, Kaliannan Ravikumar, Mahakrishnan . Palanisamy","doi":"10.56825/bufbu.2023.4223888","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4223888","url":null,"abstract":"A retrospective study was conducted in a total of 89 water buffaloes reported with cervico-vaginal prolapse (CVP) in various degrees and were initially examined for their physiological and haematological parameters. The affected buffaloes were classified into 3 groups based on the degree of CVP and were treated with 8 'R' principles and as a recurrence prevention strategy, they were managed with modified conventional vulval truss method, vulval retention suture method and both in all the three groups. The buffaloes handled with modified conventional vulval truss method showed reduced straining, genital injury and no obstetrical complications. The buffaloes treated with vulval retention suture showed mild straining, wound around the sutured site and those buffaloes treated with both modified conventional vulval truss method and vulval retention suture showed relatively effective results. Based on this study, it is concluded that the buffaloes affected with any degree of CVP and treated with modified conventional vulval truss method showed effective recurrence prevention with relatively no swelling, straining, haemorrhage, injury, maggot wound and postpartum obstetrical complications.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44606945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4225055
S. A. Shergojry, A. Verma, I. D. Gupta, N. A. Mir
The Mannose-binding lectin (MBL1) gene is an important component of immune response system. It plays vital role in activation of the complement system and act as chief defense molecule of host cell to provide protection against various diseases. In the recent study, six novel single nucleotide polymorphisms (SNPs), at 2689G>C, 2751A>G, 4822T>C, 4853A>G, 4855T>C, and 4978T>C resulted in 2 non synonymous type of change at 4853A>G, 4855T>C resulting in one amino acid substitution Ser150Gly of MBL1 protein and their association with clinical mastitis were investigated in two hundred (200) Murrah buffaloes. These SNPs in MBL1 were genotyped by using the polymerase chain reaction (PCR) and Deoxyribose nucleic acid (DNA) sequencing methods in order to reveal the association with clinical mastitis. SHEsis software tool was used for construction of haplotypes and Linkage disequilibrium analysis. Twenty one (21) haplotypes were constructed. Allelic association analysis showed that C allele of 2689 G>C, A allele of 2751 A>G, C allele of 4822 T>C, A allele of 4853 A>G, C allele of 4855 T>C, and C allele of 4978 T>C had significant association with increased risk of clinical mastitis in Murrah buffaloes (P<0.05). Murrah buffaloes with CG genotype of 2689 G>C, AG or GG genotype of 2751 A>G, CC genotype of 4822T>C, AG or GG genotype of 4853 A>G, TC or CC genotype of 4855 T>C and CC genotype of 4978 T>C loci had significantly lower incidence of clinical mastitis compared to their counter genotypes. Haplotype association analysis showed that Hap21 (TATTGA) was found significantly related to higher risk of clinical mastitis (P<0.05). Hap14 (TGTTGG) and Hap5 (CGCCCA) were found significantly associated with lower risk of clinical mastitis in Murrah buffalo (P<0.05).
{"title":"Single nucleotide polymorphism, haplotypes and genotypes of Mannose-binding lectin (MBL1) gene and their association with clinical mastitis in Murrah buffalo","authors":"S. A. Shergojry, A. Verma, I. D. Gupta, N. A. Mir","doi":"10.56825/bufbu.2023.4225055","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4225055","url":null,"abstract":"The Mannose-binding lectin (MBL1) gene is an important component of immune response system. It plays vital role in activation of the complement system and act as chief defense molecule of host cell to provide protection against various diseases. In the recent study, six novel single nucleotide polymorphisms (SNPs), at 2689G>C, 2751A>G, 4822T>C, 4853A>G, 4855T>C, and 4978T>C resulted in 2 non synonymous type of change at 4853A>G, 4855T>C resulting in one amino acid substitution Ser150Gly of MBL1 protein and their association with clinical mastitis were investigated in two hundred (200) Murrah buffaloes. These SNPs in MBL1 were genotyped by using the polymerase chain reaction (PCR) and Deoxyribose nucleic acid (DNA) sequencing methods in order to reveal the association with clinical mastitis. SHEsis software tool was used for construction of haplotypes and Linkage disequilibrium analysis. Twenty one (21) haplotypes were constructed. Allelic association analysis showed that C allele of 2689 G>C, A allele of 2751 A>G, C allele of 4822 T>C, A allele of 4853 A>G, C allele of 4855 T>C, and C allele of 4978 T>C had significant association with increased risk of clinical mastitis in Murrah buffaloes (P<0.05). Murrah buffaloes with CG genotype of 2689 G>C, AG or GG genotype of 2751 A>G, CC genotype of 4822T>C, AG or GG genotype of 4853 A>G, TC or CC genotype of 4855 T>C and CC genotype of 4978 T>C loci had significantly lower incidence of clinical mastitis compared to their counter genotypes. Haplotype association analysis showed that Hap21 (TATTGA) was found significantly related to higher risk of clinical mastitis (P<0.05). Hap14 (TGTTGG) and Hap5 (CGCCCA) were found significantly associated with lower risk of clinical mastitis in Murrah buffalo (P<0.05).","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49593993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4224146
T. Gülhan, M. Sezener, Şeyda Yaman, A. Fi̇ndi̇k, A. Çiftçi
In this study, it was aimed to develop an ELISA diagnostic kit that can be used in the serological investigation of Q fever disease caused by C. burnetii. For this purpose, bovine, sheep, goat and buffalo sera were tested with a commercial ELISA kit and home-made ELISA kit developed using two different conjugates. Of the 92 buffaloes blood serum samples tested, 22 (23.9%) were found positive by commercial ELISA kit, 7 (7.6%) and 29 (31.5%) were positive by protein-A conjugated ELISA and 29 anti-bovine IgG conjugated ELISA, respectively. In addition, Q fever positive and negative cattle, sheep, and goat blood sera that were found to be positive and negative for Q fever in a previous study conducted on animals with abortion history were also re-examined in our laboratory. In conclusion, within the scope of the project, an alternative homemade ELISA kit was developed for the serological diagnosis of Q fever and serum samples of the target species of buffalo, cattle, sheep, and goats were tested. Thus, the home-made ELISA kit, which can be used in research aimed at detecting seropositivity, especially in buffalo sera, was made ready for use. It was concluded that the data obtained would contribute to wider ranging epidemiological studies. The epidemiology of the disease can be revealed in detail in the light of research to be carried out with more comprehensive projects in our region.
{"title":"Development of ELISA kit for serological diagnosis of Q fever","authors":"T. Gülhan, M. Sezener, Şeyda Yaman, A. Fi̇ndi̇k, A. Çiftçi","doi":"10.56825/bufbu.2023.4224146","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224146","url":null,"abstract":"In this study, it was aimed to develop an ELISA diagnostic kit that can be used in the serological investigation of Q fever disease caused by C. burnetii. For this purpose, bovine, sheep, goat and buffalo sera were tested with a commercial ELISA kit and home-made ELISA kit developed using two different conjugates. Of the 92 buffaloes blood serum samples tested, 22 (23.9%) were found positive by commercial ELISA kit, 7 (7.6%) and 29 (31.5%) were positive by protein-A conjugated ELISA and 29 anti-bovine IgG conjugated ELISA, respectively. In addition, Q fever positive and negative cattle, sheep, and goat blood sera that were found to be positive and negative for Q fever in a previous study conducted on animals with abortion history were also re-examined in our laboratory. In conclusion, within the scope of the project, an alternative homemade ELISA kit was developed for the serological diagnosis of Q fever and serum samples of the target species of buffalo, cattle, sheep, and goats were tested. Thus, the home-made ELISA kit, which can be used in research aimed at detecting seropositivity, especially in buffalo sera, was made ready for use. It was concluded that the data obtained would contribute to wider ranging epidemiological studies. The epidemiology of the disease can be revealed in detail in the light of research to be carried out with more comprehensive projects in our region.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47524605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4224783
Urfeya Mirza, Sukhnandan Singh, U. Farooq
In this article, the treatment of squamous cell carcinoma surgically in an eye of an 8-year-old Nili-Ravi buffalo with entire vision loss is described. The positive result of this case indicates that radical exenteration of the orbit is a feasible option for alleviating the distress of bovines with squamous cell carcinoma of the eyes.
{"title":"Transpalpebral exenteration of eye for recurrent squamous cell carcinoma in a Nili-Ravi buffalo – A case report","authors":"Urfeya Mirza, Sukhnandan Singh, U. Farooq","doi":"10.56825/bufbu.2023.4224783","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224783","url":null,"abstract":"In this article, the treatment of squamous cell carcinoma surgically in an eye of an 8-year-old Nili-Ravi buffalo with entire vision loss is described. The positive result of this case indicates that radical exenteration of the orbit is a feasible option for alleviating the distress of bovines with squamous cell carcinoma of the eyes.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46388505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4223586
S. Ghuman, M. Honparkhe, Bilawal Singh
For assessing the impact of melatonin pre-treatment in summer anestrous Murrah buffaloes on subsequent reproductive activity of a fixed time artificial insemination (FTAI) protocol, 83 anestrous Murrah buffaloes were subcutaneously inserted 2x4 mm size absorbable melatonin (MT) implants (18 mg/50 kg b wt) at the base of left ear and 38 Murrah buffaloes were used as non-implanted controls. On day 35 post-MT, the treatment and control group Murrah buffaloes were subjected to FTAI protocol (Ovsynch plus CIDR). Ovarian ultrasonography and jugular vein blood sampling was carried out on day 7 and 10 of protocol and on day 7-post AI. Overall pregnancy rate on day 90 post-AI in MT group was higher compared to control group (55.4 vs 31.6%, P<0.05), along with the higher (P>0.05) plasma progesterone on day 7 of FTAI protocol in Murrah buffaloes of the former group which conceived subsequently compared to their non-pregnant counterparts. Moreover, MT group had higher (P>0.05) increase in dominant follicle diameter between day 7 and 10 of protocol compared to control group. On day 7 post-AI, the luteal profile in pregnant murrah buffaloes of MT group was better (P<0.05) compared to their counterparts in control group (CL: 14.80±0.70 vs 10.82±0.46 mm, P4: 1.54±0.07 vs 1.04±0.08 ng/ml). In conclusion, melatonin pre-treatment in summer anestrous Murrah buffaloes could be associated with better luteal activity and higher pregnancy rate in the Ovsynch+CIDR protocol.
为了评估夏季麻醉的Murrah水牛褪黑激素预处理对固定时间人工授精（FTI）方案后续生殖活动的影响，在左耳底部皮下植入83头麻醉的Murah水牛，植入尺寸为2x4mm的可吸收褪黑激素（MT）植入物（18 mg/50 kg b wt），38头Murrah野牛作为非植入对照。MT后第35天，治疗组和对照组Murrah水牛接受FTAI方案（Ovsynch加CIDR）。在方案的第7天、第10天和AI后第7天进行卵巢超声和颈静脉抽血。MT组在AI后第90天的总妊娠率高于对照组（55.4%对31.6%，P0.05），前者的Murrah水牛在FTI方案的第七天的血浆孕酮水平高于未怀孕的水牛。此外，与对照组相比，MT组在方案的第7天和第10天之间的优势卵泡直径增加更高（P>0.05）。在AI后第7天，MT组的妊娠水牛黄体轮廓比对照组更好（P<0.05）（CL:14.80±0.70vs10.82±0.46mm，P4:1.54±0.07vs1.04±0.08ng/ml）。总之，在Ovsynch+CIDR方案中，夏季麻醉的Murrah水牛的褪黑素预处理可能与更好的黄体活性和更高的妊娠率有关。
{"title":"Effect of melatonin implantation prior to ovsynch plus cidr protocol on subsequent ovarian activity and pregnancy rate in summer anestrous Murrah buffaloes","authors":"S. Ghuman, M. Honparkhe, Bilawal Singh","doi":"10.56825/bufbu.2023.4223586","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4223586","url":null,"abstract":"For assessing the impact of melatonin pre-treatment in summer anestrous Murrah buffaloes on subsequent reproductive activity of a fixed time artificial insemination (FTAI) protocol, 83 anestrous Murrah buffaloes were subcutaneously inserted 2x4 mm size absorbable melatonin (MT) implants (18 mg/50 kg b wt) at the base of left ear and 38 Murrah buffaloes were used as non-implanted controls. On day 35 post-MT, the treatment and control group Murrah buffaloes were subjected to FTAI protocol (Ovsynch plus CIDR). Ovarian ultrasonography and jugular vein blood sampling was carried out on day 7 and 10 of protocol and on day 7-post AI. Overall pregnancy rate on day 90 post-AI in MT group was higher compared to control group (55.4 vs 31.6%, P<0.05), along with the higher (P>0.05) plasma progesterone on day 7 of FTAI protocol in Murrah buffaloes of the former group which conceived subsequently compared to their non-pregnant counterparts. Moreover, MT group had higher (P>0.05) increase in dominant follicle diameter between day 7 and 10 of protocol compared to control group. On day 7 post-AI, the luteal profile in pregnant murrah buffaloes of MT group was better (P<0.05) compared to their counterparts in control group (CL: 14.80±0.70 vs 10.82±0.46 mm, P4: 1.54±0.07 vs 1.04±0.08 ng/ml). In conclusion, melatonin pre-treatment in summer anestrous Murrah buffaloes could be associated with better luteal activity and higher pregnancy rate in the Ovsynch+CIDR protocol.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48843166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.56825/bufbu.2023.4224514
Rahul Khurana, G. Jatav, A. Jayraw, S. Shukla, Nidhi Krishna Shrivastava, V. Agrawal, M. Shakya
In the present study, samples from 100 buffaloes of either sex were collected from slaughterhouse located at Cantonment Board Mhow for the detection of ruminal amphistomosis. Blood samples with or without anticoagulant were collected to study the haematobiochemical changes in buffaloes suffered from amphistomosis. Amphistomes were also collected for morphological identification of amphistome species in slaughtered animals. The prevalence of ruminal amphistomes was found to be 8%. The infected buffaloes in this study showed a reduction in the mean values of Hb, TEC and PCV. There was an increase in TLC and neutrophil count. Lymphocytes showed a slight decrease in their mean values and no significant changes were seen in values of basophil and eosinophil count. The infected buffaloes in this study showed a reduction in the mean values of total protein. The mean values of SGOT, SGPT and alkaline phosphatase showed an increment in the infected buffalo.
{"title":"Incidence and haematobiochemical study of amphistomosis in buffaloes (Bubalus bubalis) in Mhow, Madhya Pradesh","authors":"Rahul Khurana, G. Jatav, A. Jayraw, S. Shukla, Nidhi Krishna Shrivastava, V. Agrawal, M. Shakya","doi":"10.56825/bufbu.2023.4224514","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4224514","url":null,"abstract":"In the present study, samples from 100 buffaloes of either sex were collected from slaughterhouse located at Cantonment Board Mhow for the detection of ruminal amphistomosis. Blood samples with or without anticoagulant were collected to study the haematobiochemical changes in buffaloes suffered from amphistomosis. Amphistomes were also collected for morphological identification of amphistome species in slaughtered animals. The prevalence of ruminal amphistomes was found to be 8%. The infected buffaloes in this study showed a reduction in the mean values of Hb, TEC and PCV. There was an increase in TLC and neutrophil count. Lymphocytes showed a slight decrease in their mean values and no significant changes were seen in values of basophil and eosinophil count. The infected buffaloes in this study showed a reduction in the mean values of total protein. The mean values of SGOT, SGPT and alkaline phosphatase showed an increment in the infected buffalo.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44001848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：