Clinical hemorheology and microcirculation最新文献_第10页

An observational study of microcirculation among healthy individuals by age and sex 按年龄和性别分列的健康人微循环观察研究

Clinical hemorheology and microcirculation

Pub Date : 2023-12-07 DOI: 10.3233/ch-231807

Yun-Ping Yang, Ching-Tang Chiu, Anne Chao, Y. Yeh, Wei-Zen Sun, Chih-Min Liu, Wing-Sum Chan

OBJECTIVE: This study measured normal ranges of microcirculatory parameters in healthy individuals and investigated differences in parameters by age and sex. METHODS: Participants were enrolled into three groups with equal numbers of male and female: young (20–39 years), middle-aged (40–59 years), and elderly (60–79 years). Sublingual microcirculation images were obtained using the incident dark field (IDF). RESULTS: A total of 75 female and 75 male healthy individuals were enrolled. The elderly group had a higher TVD (26.5 [2] vs. 25.2 [1.8]; p = 0.019) and a lower PPV (97 [2] vs. 98 [3]; p = 0.03) than did the young group. In the elderly group, systolic blood pressure (SBP) and mean arterial pressure (MAP) were moderately and positively correlated with MFI score (r = 0.407, p < 0.05, and r = 0.403, p < 0.05, respectively). The female participants had a lower MFI score than did the male participants (2.9 [2.8–3] vs. 3.0 [2.9–3]; p = 0.015). CONCLUSIONS: This study revealed the range of microcirculatory parameters between different ages and sexes in healthy individuals. We found that blood pressure levels were correlated with microcirculatory parameters, especially in elders and female.

目的:本研究测量了健康人微循环参数的正常范围，并研究了不同年龄和性别的参数差异。方法:参与者被分为男女人数相等的三组:年轻人(20-39岁)、中年人(40-59岁)和老年人(60-79岁)。使用入射暗场(IDF)获得舌下微循环图像。结果:共纳入75名女性和75名男性健康个体。老年组TVD较高(26.5 [2]vs. 25.2 [1.8];p = 0.019)和较低的PPV(97[2]对98 [3];P = 0.03)。老年组收缩压(SBP)、平均动脉压(MAP)与MFI评分呈中度正相关(r = 0.407, p < 0.05; r = 0.403, p < 0.05)。女性受试者的MFI得分低于男性受试者(2.9 [2.8-3]vs. 3.0 [2.9 - 3]);p = 0.015)。结论:本研究揭示了健康人不同年龄和性别之间微循环参数的变化范围。我们发现血压水平与微循环参数相关，尤其是在老年人和女性中。

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引用次数: 0

Modulation of the capillary leakage by exogenous albumin in a rat model of endothelial glycocalyx damage 大鼠内皮糖萼损伤模型中外源性白蛋白对毛细血管渗漏的调节作用

Clinical hemorheology and microcirculation

Pub Date : 2023-12-07 DOI: 10.3233/ch-232027

D. Astapenko, Marek Zrzavecky, Diana Gorskaja, R. Hyšpler, A. Tichá, Vera Radochova, Christian Lehmann, Manu L. N. G. Malbrain, Vladimir Cerny, Robert G. Hahn

BACKGROUND: Endothelial glycocalyx (EG) plays a crucial role in maintaining the plasma proteins within the intravascular space. OBJECTIVE: We studied whether exogenous albumin protects the EG in an experimental model of EG enzymatic damage in rats. METHODS: Rats were divided into three groups of 10 animals that received (1) Evans blue (2) Evans blue + hyaluronidase, or (3) Evans blue + hyaluronidase + 20% human albumin via the tail vein. Spectrophotometric analysis was performed 2 h later to quantify the leakage of Evans blue-labeled albumin into the heart, lungs, brain, kidneys, liver, small intestine, spleen, and skeletal muscle. RESULTS: Administration of hyaluronidase numerically increased the capillary leakage of Evans blue in all examined tissues. Co-administration of albumin decreased the leakage of albumin in all tissues except the heart. In the lungs, the ratio between the absorbance and dry organ weight decreased from 5.3 ± 2.4 to 1.7 ± 0.5 (mean ± SD) (P < 0.002), and in the liver, the absorbance decreased from 2.2 ± 0.7 to 1.5 ± 0.4 (P < 0.011). CONCLUSION: Exogenous albumin decreased the capillary leakage of albumin which was interpreted as a sign of maintained EG integrity.

背景:内皮糖萼(Endothelial glycocalyx, EG)在维持血浆蛋白在血管内空间中起着至关重要的作用。目的:研究外源性白蛋白对大鼠EG酶损伤的保护作用。方法:将大鼠分为3组，每组10只，分别经尾静脉注射(1)埃文斯蓝(2)埃文斯蓝+透明质酸酶，或(3)埃文斯蓝+透明质酸酶+ 20%人白蛋白。2 h后进行分光光度分析，量化Evans蓝色标记白蛋白渗漏到心脏、肺、脑、肾脏、肝脏、小肠、脾脏和骨骼肌的情况。结果:在所有检查组织中，给药透明质酸酶增加了埃文斯蓝毛细血管渗漏。同时给予白蛋白可减少除心脏外所有组织的白蛋白渗漏。肺吸光度与干脏器重之比由5.3±2.4降至1.7±0.5 (mean±SD) (P < 0.002)，肝吸光度由2.2±0.7降至1.5±0.4 (P < 0.011)。结论:外源性白蛋白减少了白蛋白的毛细血管渗漏，这被解释为维持EG完整性的标志。

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引用次数: 0

Blood rheology and vascular function in sickle cell trait and sickle cell disease: From pathophysiological mechanisms to clinical usefulness 镰状细胞性状和镰状细胞病的血液流变学和血管功能：从病理生理机制到临床实用性

Clinical hemorheology and microcirculation

Pub Date : 2023-12-07 DOI: 10.3233/ch-238122

P. Connes

Sickle cell disease (SCD) is an autosomal recessive disorder. Although the molecular mechanisms at the origin of SCD have been well characterized, its clinical expression is highly variable. SCD is characterized by blood rheological abnormalities, increased inflammation and oxidative stress, and vascular dysfunction. Individuals with only one copy of the mutated β-globin gene have sickle cell trait (SCT) and are usually asymptomatic. The first part of this review focuses on the biological responses of SCT carriers during exercise and on the effects of combined SCT and diabetes on vascular function, several biomarkers and clinical complications. The second part of the review focuses on SCD and shows that the magnitude of red blood cell (RBC) rheological alterations is highly variable from one patient to another, and this variability reflects the clinical and hematological variability: patients with the less deformable RBCs have high hemolytic rate and severe anemia, and are prone to develop leg ulcers, priapism, cerebral vasculopathy, glomerulopathy or pulmonary hypertension. In contrast, SCD patients characterized by the presence of more deformable RBCs (but still rigid) are less anemic and may exhibit increased blood viscosity, which increases the risk for vaso-occlusive events. Several genetic and cellular factors may modulate RBC deformability in SCD: co-existence of α-thalassemia, fetal hemoglobin level, oxidative stress, the presence of residual mitochondria into mature RBCs, the activity of various non-selective cationic ion channels, etc. The last part of this review presents the effects of hydroxyurea and exercise training on RBC rheology and other biomarkers in SCD.

镰状细胞病是一种常染色体隐性遗传病。虽然SCD起源的分子机制已经被很好地描述，但其临床表现却变化很大。SCD的特点是血液流变学异常，炎症和氧化应激增加，血管功能障碍。只有一个突变β-珠蛋白基因拷贝的个体具有镰状细胞特征(SCT)，通常无症状。第一部分综述了SCT携带者在运动中的生物学反应，以及SCT联合糖尿病对血管功能、几种生物标志物和临床并发症的影响。该综述的第二部分重点关注SCD，并表明红细胞流变学改变的幅度在不同患者之间存在很大差异，这种变异性反映了临床和血液学的变异性:红细胞变形较小的患者溶血率高，贫血严重，并且容易发生腿部溃疡、阴茎勃起、脑血管病、肾小球病或肺动脉高压。相反，以红细胞更易变形(但仍然坚硬)为特征的SCD患者贫血程度较低，并可能表现出血液粘度增加，这增加了血管闭塞事件的风险。几个遗传和细胞因素可能调节SCD红细胞的变形性:α-地中海贫血的共存、胎儿血红蛋白水平、氧化应激、成熟红细胞中残余线粒体的存在、各种非选择性阳离子离子通道的活性等。最后介绍了羟基脲和运动训练对SCD红细胞流变学和其他生物标志物的影响。

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引用次数: 0

Thrombosis density ratio can predict the occurrence of pulmonary embolism and post-thrombotic syndrome in lower-extremity deep vein thrombosis patients 血栓密度比可预测下肢深静脉血栓患者肺栓塞和血栓后综合征的发生率

Clinical hemorheology and microcirculation

Pub Date : 2023-12-07 DOI: 10.3233/ch-231778

Miao-Miao Yao, Ting-Ting Lin, Xu Shi, Mei-Gui Chen, Jia-Xiang Wu, Yan-Ping Zhao, Bao-Shan Lin

OBJECTIVE: Deep vein thrombosis (DVT) formation of lower extremities can lead to serious complications including pulmonary embolism (PE) and chronic post-thrombotic syndrome (PTS). We aimed to explore the relationship between the ratio of thrombotic density and the occurrence of PE and PTS in patients with DVT of the lower extremities. METHODS: A retrospective analysis was conducted in patients who performed computed tomography venography, dividing into DVT with PE group (54 patients) and DVT-alone group (34 patients), The clinical data were recorded. Univariate and multivariate logistic regression analysis were used to analysis variables associated with PE. The ability of thrombosis density ratio and Wells score to diagnose PE was evaluated by using the receiver operating characteristic curve (ROC) area under the curve (AUC). According to the treatment and follow-up results, subgroup analysis was performed, and the Villata score was used to determine the presence or absence of PTS and its severity. RESULTS: Compare with the DVT-alone group, more patients had dyspnea and chest pain in the DVT with PE group. DVT with PE group had lower the percentage of neutrophils, white blood cell count and platelet count, while had higher blood cell count, D-dimer, wells score, thrombus and thrombus density ratio. Multivariate logistic analysis showed that percentage of neutrophils (OR(95% CIs)=1.15 (1.01,1.31), P = 0.040), platelets (OR(95% CIs)=0.96 (0.93,0.99), P = 0.011), and thrombus density ratio (OR(95% CIs)=5.99 (1.96,18.35), P = 0.002) are independent predictors of PE. The Wells score and thrombosis density ratio were consistent in the diagnostic efficacy of PE. In the subgroup analysis, there was a relevance between the ratio of thrombosis density and the Villalta score. CONCLUSION: Percentage of neutrophils, platelets, and thrombus density ratio are independent predictors of PE. The thrombosis density of DVT patients may be an index to predict the risk of PE and PTS in DVT patients.

目的:下肢深静脉血栓形成(DVT)可导致严重并发症，包括肺栓塞(PE)和慢性血栓后综合征(PTS)。我们旨在探讨下肢DVT患者血栓密度比与PE和PTS发生的关系。方法:回顾性分析行ct静脉造影的患者，将其分为DVT合并PE组(54例)和DVT单独组(34例)，记录临床资料。采用单因素和多因素logistic回归分析与PE相关的变量。采用受试者工作特征曲线(ROC)曲线下面积(AUC)评价血栓密度比和Wells评分对PE的诊断能力。根据治疗及随访结果进行亚组分析，采用Villata评分法判断有无PTS及其严重程度。结果:与单纯DVT组相比，DVT合并PE组出现呼吸困难、胸痛的患者较多。DVT合并PE组中性粒细胞百分比、白细胞计数和血小板计数较低，血细胞计数、d -二聚体、井评分、血栓和血栓密度比较高。多因素logistic分析显示，中性粒细胞百分比(OR(95% CIs)=1.15 (1.01,1.31)， P = 0.040)、血小板(OR(95% CIs)=0.96 (0.93,0.99)， P = 0.011)、血栓密度比(OR(95% CIs)=5.99 (1.96,18.35)， P = 0.002)是PE的独立预测因子。Wells评分和血栓密度比对PE的诊断效果一致。在亚组分析中，血栓密度比与Villalta评分之间存在相关性。结论:中性粒细胞百分比、血小板百分比和血栓密度比是PE的独立预测因子。深静脉血栓形成密度可作为预测深静脉血栓患者发生PE和PTS风险的指标。

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引用次数: 0

Effectiveness of an online/offline mixed-mode Tai Chi cardiac rehabilitation program on microcirculation in patients with coronary artery disease: A randomized controlled study. 线上/线下混合模式太极心脏康复计划对冠状动脉疾病患者微循环的有效性：一项随机对照研究。

Clinical hemorheology and microcirculation

Pub Date : 2023-01-01 DOI: 10.3233/CH-231894

Shaojun Lyu, Zaihao Chen, Meize Cui, Qiuyang Wei, Yameng Li, Hui Fang, Mingyu Liu, Linli Liu, Jiahao Fu, Jianwei Zhang

Objective: We explored the effectiveness of an online/offline mixed-mode Tai Chi cardiac rehabilitation program on the microcirculation of patients with coronary artery disease (CAD).

Design: Prospective, randomized controlled study.

Setting: It was conducted in a tertiary hospital.

Subjects: Twenty-six patients who met the diagnostic criteria for coronary artery disease were recruited.

Interventions: Patients were randomized divided into a 12-week Tai Chi cardiac rehabilitation program(TCCRP) or a conventional exercise rehabilitation program(CERP) in a 1:1 fashion, 4 weeks of in-hospital rehabilitation and 8 weeks of online rehabilitation at home (a total of 12 weeks of intervention).

Main outcome measures: Nailfold microcirculation (Morphological integrals, Blood flow integrals, Periphery capillary loop integrals, Overall integrals).

Main outcome measures: Twenty patients completed the study. The Morphological integrals (baseline: 2.875±1.171 vs 12weeks: 1.863±0.414, t = 2.432, P = 0.045 < 0.05) and Overall integrals (baseline: 5.563±2.001 vs 12weeks: 3.688±1.167, t = 3.358, P = 0.012 < 0.05) decreased significantly in the TCCRP, The nailfold microcirculation integra decreased not significantly in the CERP (P > 0.05). The nailfold microcirculation integra was not significantly different between the two groups after the intervention (P > 0.05).

Conclusions: The TCCRP improved the microcirculation of patients with CAD.

目的：探讨线上/线下混合太极心脏康复方案对冠心病患者微循环的影响。设计：前瞻性随机对照研究。设置：在一家三级医院进行。受试者：26名符合冠状动脉疾病诊断标准的患者被招募。干预措施：患者以1:1的方式随机分为12周太极心脏康复计划（TCCRP）或常规运动康复计划（CERP），4周的住院康复和8周的家庭在线康复（共12周的干预）。主要结果指标：甲襞微循环（形态积分、血流积分、外周毛细血管环积分、整体积分）。主要成果指标：20名患者完成了研究。形态学积分（基线：2.875±1.171 vs 12周：1.863±0.414，t = 2.432，P = 0.045 < 0.05）和总积分（基线：5.563±2.001 vs 12周：3.688±1.167，t = 3.358，P = 0.012 < 0.05）显著降低，甲襞微循环积分在CERP中下降不显著（P > 干预后两组甲襞微循环积分差异无统计学意义（P > 结论：TCCRP可改善冠心病患者的微循环。

{"title":"Effectiveness of an online/offline mixed-mode Tai Chi cardiac rehabilitation program on microcirculation in patients with coronary artery disease: A randomized controlled study.","authors":"Shaojun Lyu, Zaihao Chen, Meize Cui, Qiuyang Wei, Yameng Li, Hui Fang, Mingyu Liu, Linli Liu, Jiahao Fu, Jianwei Zhang","doi":"10.3233/CH-231894","DOIUrl":"10.3233/CH-231894","url":null,"abstract":"Objective: We explored the effectiveness of an online/offline mixed-mode Tai Chi cardiac rehabilitation program on the microcirculation of patients with coronary artery disease (CAD).Design: Prospective, randomized controlled study.Setting: It was conducted in a tertiary hospital.Subjects: Twenty-six patients who met the diagnostic criteria for coronary artery disease were recruited.Interventions: Patients were randomized divided into a 12-week Tai Chi cardiac rehabilitation program(TCCRP) or a conventional exercise rehabilitation program(CERP) in a 1:1 fashion, 4 weeks of in-hospital rehabilitation and 8 weeks of online rehabilitation at home (a total of 12 weeks of intervention).Main outcome measures: Nailfold microcirculation (Morphological integrals, Blood flow integrals, Periphery capillary loop integrals, Overall integrals).Main outcome measures: Twenty patients completed the study. The Morphological integrals (baseline: 2.875±1.171 vs 12weeks: 1.863±0.414, t = 2.432, P = 0.045 < 0.05) and Overall integrals (baseline: 5.563±2.001 vs 12weeks: 3.688±1.167, t = 3.358, P = 0.012 < 0.05) decreased significantly in the TCCRP, The nailfold microcirculation integra decreased not significantly in the CERP (P > 0.05). The nailfold microcirculation integra was not significantly different between the two groups after the intervention (P > 0.05).Conclusions: The TCCRP improved the microcirculation of patients with CAD.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41149819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A.L. Copley Best Paper Prize 2022. A.L. Copley 最佳论文奖 2022.

Clinical hemorheology and microcirculation

Pub Date : 2023-01-01 DOI: 10.3233/CH-239103

Friedrich Jung, Philippe Connes, Christian Lehmann

引用次数: 0

Dynamic contrast-enhanced ultrasound characteristics of renal tumors: VueBox™ quantitative analysis. 肾肿瘤的动态增强超声特征：VueBox™ 定量分析。

Clinical hemorheology and microcirculation

Pub Date : 2023-01-01 DOI: 10.3233/CH-231750

Ning Li, Ziyue Hu, Yang Liu, Jianmin Ding, Pengcheng Han, Xiang Jing, Yanmin Kan

Background: VueBoxtrademark has been used for contrast analysis in DCE-US-based quantitative research.

Objective: Aim of this study was to use the enhancement-mode and VueBoxtrademark parameters to further evaluate the differential diagnostic value of DCE-US for renal tumors.

Methods: 24 patients with renal tumors, including 7 benign and 17 malignant, were retrospectively analyzed.The DCE-US enhancement-mode and VueBoxtrademark parameters correlated with the histological analyses of tumors were obtained and analyzed.

Results: The benign and malignant renal tumors showed significant differences in enhancement degree (P = 0.017) and presence of a pseudocapsule (P = 0.009) and in the VueBoxtrademark parameters FT (P = 0.045) and RT (P = 0.039). Receiver operating characteristic analysis for differential diagnosis of benign and malignant renal tumors showed that AUC for a combination of enhancement degree and presence of a pseudocapsule was greater (AUC = 0.815) than the values for either parameter of enhancement mode alone. Similarly, the AUC for a combination of RT and FT was greater (AUC = 0.798) than the values for RT or FT alone. A comprehensive index obtaining by combining the enhancement-mode and VueBoxtrademark parameters showed the largest AUC (AUC = 0.916) with relatively high accuracy (87.50%), sensitivity (76.50%), and specificity (85.70%).

Conclusions: DCE-US with enhancement mode and quantitative analysis can facilitate preoperative differential diagnosis of benign and malignant renal tumors.

背景：VueBox商标已用于DCE US定量研究中的对比分析。目的：利用增强模式和VueBox商标参数进一步评价DCE-US对肾肿瘤的鉴别诊断价值。方法：对24例肾肿瘤患者进行回顾性分析，其中良性肿瘤7例，恶性肿瘤17例。获得并分析了与肿瘤组织学分析相关的DCE-US增强模式和VueBox商标参数。结果：肾良恶性肿瘤增强程度差异有统计学意义（P = 0.017）和假包囊的存在（P = 0.009）和VueBox商标参数FT（P = 0.045）和RT（P = 0.039）。用于鉴别诊断良性和恶性肾肿瘤的受试者操作特征分析显示，增强程度和假包膜存在的组合的AUC更大（AUC = 0.815）的值。类似地，RT和FT组合的AUC更大（AUC = 0.798）的值。通过组合增强模式和VueBox商标参数获得的综合指数显示出最大的AUC（AUC = 0.916），具有较高的准确性（87.50%）、敏感性（76.50%）和特异性（85.70%）。结论：DCE-US具有增强模式和定量分析，有助于肾良恶性肿瘤的术前鉴别诊断。

{"title":"Dynamic contrast-enhanced ultrasound characteristics of renal tumors: VueBox™ quantitative analysis.","authors":"Ning Li, Ziyue Hu, Yang Liu, Jianmin Ding, Pengcheng Han, Xiang Jing, Yanmin Kan","doi":"10.3233/CH-231750","DOIUrl":"10.3233/CH-231750","url":null,"abstract":"Background: VueBoxtrademark has been used for contrast analysis in DCE-US-based quantitative research.Objective: Aim of this study was to use the enhancement-mode and VueBoxtrademark parameters to further evaluate the differential diagnostic value of DCE-US for renal tumors.Methods: 24 patients with renal tumors, including 7 benign and 17 malignant, were retrospectively analyzed.The DCE-US enhancement-mode and VueBoxtrademark parameters correlated with the histological analyses of tumors were obtained and analyzed.Results: The benign and malignant renal tumors showed significant differences in enhancement degree (P = 0.017) and presence of a pseudocapsule (P = 0.009) and in the VueBoxtrademark parameters FT (P = 0.045) and RT (P = 0.039). Receiver operating characteristic analysis for differential diagnosis of benign and malignant renal tumors showed that AUC for a combination of enhancement degree and presence of a pseudocapsule was greater (AUC = 0.815) than the values for either parameter of enhancement mode alone. Similarly, the AUC for a combination of RT and FT was greater (AUC = 0.798) than the values for RT or FT alone. A comprehensive index obtaining by combining the enhancement-mode and VueBoxtrademark parameters showed the largest AUC (AUC = 0.916) with relatively high accuracy (87.50%), sensitivity (76.50%), and specificity (85.70%).Conclusions: DCE-US with enhancement mode and quantitative analysis can facilitate preoperative differential diagnosis of benign and malignant renal tumors.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between conventional ultrasound features combined with contrast-enhanced ultrasound patterns and pathological prognostic factors in malignant non-mass breast lesions. 乳腺恶性非肿块病变的常规超声特征与超声造影模式及病理预后因素的相关性。

Clinical hemorheology and microcirculation

Pub Date : 2023-01-01 DOI: 10.3233/CH-231936

Tong Wang, Wenjuan Guo, Xuemei Zhang, Ji Ma, Fang Li, Siqi Zheng, Miao Zhu, Yi Dong, Min Bai

Objective: To investigate the correlation between ultrasound performance and prognostic factors in malignant non-mass breast lesions (NMLs).

Materials and methods: This study included 106 malignant NMLs in 104 patients. Different US features and contrast enhancement patterns were evaluated. Prognostic factors, including histological types and grades, axillary lymph node and peritumoral lymphovascular status, estrogen and progesterone receptor status and the expression of HER-2 and Ki-67 were determined. A chi-square test and logistic regression analysis were used to analyse possible associations.

Results: Lesion size (OR: 3.08, p = 0.033) and posterior echo attenuation (OR: 8.38, p < 0.001) were useful in reflecting malignant NMLs containing an invasive carcinoma component. Posterior echo attenuation (OR: 7.51, p = 0.003) and unclear enhancement margin (OR: 6.50, p = 0.018) were often found in tumors with axillary lymph node metastases. Peritumoural lymphovascular invasion mostly exhibited posterior echo attenuation (OR: 3.84, p = 0.049) and unclear enhancement margin (OR: 8.68, p = 0.042) on ultrasound images. Perfusion defect was a comparatively accurate enhancement indicator for negative ER (OR: 2.57, p = 0.041) and PR (OR: 3.04, p = 0.008) expression. Calcifications (OR: 3.03, p = 0.025) and enlarged enhancement area (OR: 5.36, p = 0.033) imply an increased risk of positive HER-2 expression. Similarly, Calcifications (OR: 4.13, p = 0.003) and enlarged enhancement area (OR: 11.05, p < 0.001) were valid predictors of high Ki-67 proliferation index.

Conclusion: Ultrasound performance is valuable for non-invasive prediction of prognostic factors in malignant NMLs.

目的：探讨乳腺非肿块性恶性病变的超声表现与预后因素的相关性。评估了不同的US特征和对比度增强模式。测定预后因素，包括组织学类型和分级、腋窝淋巴结和肿瘤周围淋巴管状态、雌激素和孕激素受体状态以及HER-2和Ki-67的表达。卡方检验和逻辑回归分析用于分析可能的关联。结果：病变大小（OR:3.08，p = 0.033）和后回声衰减（OR:8.38，p < 0.001）可用于反映含有侵袭性癌成分的恶性NML。后部回声衰减（OR:7.51，p = 0.003）和不清楚的增强边缘（OR:6.50，p = 0.018）在伴有腋窝淋巴结转移的肿瘤中经常发现。肿瘤周围淋巴管侵犯主要表现为后部回声衰减（OR:3.84，p = 0.049）和不清楚的增强边缘（OR:8.68，p = 0.042）。灌注缺陷是ER阴性的一个相对准确的增强指标（OR:2.57，p = 0.041）和PR（OR:3.04，p = 0.008）表达。钙化（OR:3.03，p = 0.025）和增强面积增大（OR:5.36，p = 0.033）意味着HER-2阳性表达的风险增加。类似地，钙化（OR:4.13，p = 0.003）和增强区增大（OR:11.05，p < 0.001）是高Ki-67增殖指数的有效预测因子。结论：超声表现有助于无创预测恶性NML的预后因素。

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引用次数: 0

CircRBM33 induces endothelial dysfunction by targeting the miR-6838-5p/PDCD4 axis affecting blood-brain barrier in mice with cerebral ischemia-reperfusion injury. CircRBM33通过靶向影响脑缺血再灌注损伤小鼠血脑屏障的miR-6838-5p/PDCD4轴诱导内皮功能障碍。

Clinical hemorheology and microcirculation

Pub Date : 2023-01-01 DOI: 10.3233/CH-231776

Yanbin Zhang, Xiaodong Yuan, Jie Xu, Huafen Gu

Background: circRNAs (circRNAs) are involved in the process of cerebral ischemia-reperfusion injury (CI/RI). Our study aims to explore circRBM33 in the endothelial function of the blood-brain barrier (BBB).

Methods: The mouse middle cerebral artery occlusion model (MCAO) was established and restored to perfusion, and OGD/R-induced endothelial cells were used to simulate CI/RI. circRBM33, miR-6838-5p and PDCD4, as well as Occludin, ZO-1 and Claudin-5 TJs were evaluated by quantitative PCR and Western blot. The ring structure of circRBM33 was verified by RNAse R and actinomycin D experiments. MTT and LDH Cytotoxicity assay determined viability and toxicity, and flow cytometry determined apoptosis rate. Inflammatory cytokines and the number of microglia in brain tissue were measured by ELISA and IHC. The interaction between genes was verified by RIP and dual luciferase reporter assay.

Results: circRBM33 was a circrRNA present in the cytoplasm and up-regulated in the brain tissue of MCAO mice and OGD/R-induced endothelial cells. Silenced circRBM33 promoted Occludin, ZO-1, and Claudin-5 expression and cell proliferation, and inhibited cytotoxicity, inflammatory response, and apoptosis. Functionally, circRBM33-absorbed miR-6838-5p was involved in regulating PDCD4, leading to endothelial cell dysfunction, and thus affecting the function of the BBB.

Conclusions: circRBM33 by mediating miR-6838-5p/PDCD4 axis induces endothelial dysfunction, thereby affecting the BBB in mice with CI/RI.

背景：circRNAs（circRNAs）参与了脑缺血再灌注损伤（CI/RI）的过程。我们的研究旨在探索circRBM33在血脑屏障（BBB）内皮功能中的作用。方法：建立小鼠大脑中动脉闭塞模型（MCAO）并恢复灌流，用OGD/R诱导的内皮细胞模拟CI/RI。通过定量PCR和蛋白质印迹评估circRBM33、miR-6838-5p和PDCD4，以及Occludin、ZO-1和Claudin-5TJs。通过RNAse R和放线菌素D实验验证了circRBM33的环结构。MTT和LDH细胞毒性试验测定细胞活力和毒性，流式细胞术测定细胞凋亡率。采用ELISA法和IHC法测定脑组织中炎性细胞因子和小胶质细胞数量。通过RIP和双荧光素酶报告基因分析验证了基因之间的相互作用。结果：circRBM33是一种存在于MCAO小鼠脑组织和OGD/R诱导的内皮细胞中的circrRNA。沉默的circRBM33促进Occludin、ZO-1和Claudin-5的表达和细胞增殖，并抑制细胞毒性、炎症反应和细胞凋亡。在功能上，circRBM33吸收的miR-6838-5p参与调节PDCD4，导致内皮细胞功能障碍，从而影响血脑屏障的功能。结论：circRBM33通过介导miR-6838-5p/PDCD4轴诱导内皮功能障碍，从而影响CI/RI小鼠的血脑屏障。

{"title":"CircRBM33 induces endothelial dysfunction by targeting the miR-6838-5p/PDCD4 axis affecting blood-brain barrier in mice with cerebral ischemia-reperfusion injury.","authors":"Yanbin Zhang, Xiaodong Yuan, Jie Xu, Huafen Gu","doi":"10.3233/CH-231776","DOIUrl":"10.3233/CH-231776","url":null,"abstract":"Background: circRNAs (circRNAs) are involved in the process of cerebral ischemia-reperfusion injury (CI/RI). Our study aims to explore circRBM33 in the endothelial function of the blood-brain barrier (BBB).Methods: The mouse middle cerebral artery occlusion model (MCAO) was established and restored to perfusion, and OGD/R-induced endothelial cells were used to simulate CI/RI. circRBM33, miR-6838-5p and PDCD4, as well as Occludin, ZO-1 and Claudin-5 TJs were evaluated by quantitative PCR and Western blot. The ring structure of circRBM33 was verified by RNAse R and actinomycin D experiments. MTT and LDH Cytotoxicity assay determined viability and toxicity, and flow cytometry determined apoptosis rate. Inflammatory cytokines and the number of microglia in brain tissue were measured by ELISA and IHC. The interaction between genes was verified by RIP and dual luciferase reporter assay.Results: circRBM33 was a circrRNA present in the cytoplasm and up-regulated in the brain tissue of MCAO mice and OGD/R-induced endothelial cells. Silenced circRBM33 promoted Occludin, ZO-1, and Claudin-5 expression and cell proliferation, and inhibited cytotoxicity, inflammatory response, and apoptosis. Functionally, circRBM33-absorbed miR-6838-5p was involved in regulating PDCD4, leading to endothelial cell dysfunction, and thus affecting the function of the BBB.Conclusions: circRBM33 by mediating miR-6838-5p/PDCD4 axis induces endothelial dysfunction, thereby affecting the BBB in mice with CI/RI.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71489987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0