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Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology最新文献

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Morphological study of distal furcation in mandibular first molars based on cone beam computed tomography images in Beijing population. 基于锥形束计算机断层扫描图像的北京人群下颌第一磨牙远端窝沟形态学研究。
Jie Zhu, Ya Liu

Objectives: This retrospective study explored the incidence of independent distal-lingual root (DLR) in mandibular first molars and the morphologic features of distal furcations through cone beam computed tomography (CBCT) in Beijing population.

Methods: A total of 401 CBCT images of both mandibular first molars existed were included. The sex of the patients, the incidence of DLR, the distal root trunk length (DRT), the locations of distal furcation entrance (DFE), and the separation angle of distal furcation (ADF) were recorded.

Results: The incidence rates of DLR were 33.2% on the subject level and 26.8% on the tooth level. The incidence of DLR was not different between genders but was higher on the right sides. The DRT values were 4.15 mm±1.02 mm. The ADF was 65.56°±11.56°. The DFE was located lingually, and 97.2% DFE was located more apically than buccal/lingual furcations.

Conclusions: A high incidence of DLR was found in the Beijing population. The DRT was longer than buccal and lingual sites. The DFE was located lingually with a wide separation. Understanding the morphological features of distal furcation can help clinicians to make proper treatment plans.

研究目的这项回顾性研究通过锥形束计算机断层扫描(CBCT)探讨了北京人群中下颌第一磨牙独立远端舌根(DLR)的发生率和远端毛囊的形态特征:方法:共纳入 401 张下颌第一磨牙的 CBCT 图像。方法:共纳入 401 张下颌第一磨牙 CBCT 图像,记录患者性别、DLR 发生率、远端根干长度(DRT)、远端窝沟入口位置(DFE)和远端窝沟分离角(ADF):受试者的 DLR 发生率为 33.2%,牙齿的 DLR 发生率为 26.8%。不同性别的 DLR 发生率没有差异,但右侧较高。DRT值为4.15 mm±1.02 mm。ADF为65.56°±11.56°。DFE位于舌侧,97.2%的DFE位于比颊/舌沟更尖的位置:结论:在北京人群中,DLR的发病率很高。结论:在北京人群中,DLR 的发病率很高。DFE位于舌侧,分隔较宽。了解远端窝沟的形态特征有助于临床医生制定正确的治疗方案。
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引用次数: 0
Correlation between FOXN3-SIN3A complex expression in peripheral blood and non-syndromic cleft lip and palate in Xinjiang. 新疆外周血中 FOXN3-SIN3A 复合物表达与非综合征唇腭裂的相关性。
Duolikun Wufuer, Dilibaier Yimingjiang, Kamilijiang Maimaitiming, Jun Li, Wulifan Tuoleheng

Objectives: This work aimed to study the correlation between FOXN3-SIN3A complex expression and non-syndromic oral clefts (NSOC) in Xinjiang.

Methods: In this study, 60 patients with NSOC attending the People's Hospital of Xinjiang Uygur Autonomous Region were recruited into the case group, including 30 cleft lip with or without cleft palate (NSCL/P), 30 cleft palate only (CPO), and 30 healthy children in the control group. The expression levels of FOXN3, SIN3A, and NEAT1 in peripheral blood of each group were detected by high-throughput second-generation sequencing technology and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to analyze the diagnostic efficiency of NSOC.

Results: The comparison of the NSOC and control groups showed that FOXN3, SIN3A, and NEAT1 genes increased compared with the control group. The differences were all statistically significant (P<0.05). The AUCs of FOXN3, SIN3A, and NEAT1 in the NSCL/P group were 0.933 [95%CI=(0.864, 1.000)], 0.822 [(95%CI=(0.713, 0.932)], and 1.000[95%CI= (1.000, 1.000)], respectively. The AUCs of FOX-N3, SIN3A, and NEAT1 in the CPO group were 0.891 [95%CI=(0.806, 0.976)], 0.688 [95%CI=(0.552, 0.824)], and 1.000 [95%CI=(1.000, 1.000)], respectively.

Conclusions: The results showed a correlation between the rising gene expression of FOXN3, SIN3A, and NEAT1 in peripheral blood and the occurrence of NSOC in Xinjiang. This work provides a theoretical basis for further study of the FOXN3-SIN3A complex as biomarkers to facilitate the early screening, disease prediction, and early prevention of NSOC.

研究目的本研究旨在探讨FOXN3-SIN3A复合体表达与新疆非综合征性口腔裂隙(NSOC)的相关性:方法:本研究选取在新疆维吾尔自治区人民医院就诊的60例NSOC患者为病例组,其中唇裂伴或不伴腭裂(NSCL/P)30例,单纯腭裂(CPO)30例,对照组为30例健康儿童。通过高通量二代测序技术和定量反转录聚合酶链反应(RT-qPCR)检测各组外周血中 FOXN3、SIN3A 和 NEAT1 的表达水平。采用接收者操作特征曲线(ROC)和曲线下面积(AUC)分析NSOC的诊断效率:结果:NSOC组与对照组的比较显示,FOXN3、SIN3A和NEAT1基因比对照组增高。结果:与对照组相比,NSOC 组的 FOXN3、SIN3A 和 NEAT1 基因均有所增加,差异均有统计学意义(PConclusions:结果表明,外周血中 FOXN3、SIN3A 和 NEAT1 基因表达的升高与新疆 NSOC 的发生存在相关性。这项工作为进一步研究 FOXN3-SIN3A 复合物作为生物标志物,促进 NSOC 的早期筛查、疾病预测和早期预防提供了理论依据。
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引用次数: 0
Chinese surgical robot-assisted surgery for parotid tumor: a case report. 中国手术机器人辅助腮腺肿瘤手术:病例报告。
Guiquan Zhu, Zhongkai Ma, Chang Cao, Jialu He, Jiawei Hong, Ruiting Ren, Hui Xia, Bing Yan, Xiaoyi Wang, Long-Jiang Li, Chunjie Li
Robotic surgery is known as the "third technological revolution" in the field of surgery, and is an important milestone in the development of modern surgery. However, our country's innovative surgical robot industry is still in its early stages, and it is only being utilized in certain surgical fields. To explore the effectiveness of the application of domestic surgical robot in oral and maxillofacial surgery, the author successfully completed a case of benign parotid tumor resection with the assistance of a domestic autonomous robot. The operation was successful, facial nerve function was preserved, and postoperative wound healing was good.
机器人手术被称为外科领域的 "第三次技术革命",是现代外科发展的重要里程碑。然而,我国的手术机器人创新产业仍处于初级阶段,仅在部分手术领域得到应用。为探讨国产手术机器人在口腔颌面外科的应用效果,笔者在国产自主机器人的辅助下成功完成了一例良性腮腺肿瘤切除术。手术成功,面神经功能得以保留,术后伤口愈合良好。
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引用次数: 0
Site selection of micro-implant anchorages in the infrazygomatic crest in adult orthodontic patients. 成年正畸患者颧骨下微型种植体固定的位置选择。
Xinyi Chen, Xiaoge Jiang, Song Chen
OBJECTIVESTo determine the optimal placement of miniscrews, this study compared adult male and female patients in terms of cortical bone density, cortical bone thickness, and available bone width in the infrazygomatic crest region.METHODSThe cone beam computed tomography imaging data of 200 patients (20-30 years old; 100 males and 100 females) were collected. The right maxillary posterior teeth in the sagittal plane were divided into six levels from proximal to distal, and three measurement sites were positioned at vertical distances of 8, 10, and 12 mm from the cementum. Cortical bone density, cortical bone thickness, and available bone width were measured in 18 measurement sites in the infrazygomatic crest and analyzed statistically.RESULTSThe highest cortical bone density, cortical bone thickness, and available bone width in the infrazygomatic crest in adult male and female patients were at the level of the interradicular space between the maxillary second premolar and maxillary first molar. The bone cortical density and thickness increased with vertical height, whereas the available bone width decreased with increasing vertical height. Differences were observed in cortical bone density, cortical bone thickness, and available bone width between adult male and female patients.CONCLUSIONSThe optimal implantation sites of the micro-implant anchorages in the infrazygomatic crest were at the level of the interradicular space between the maxillary second premolar and the maxillary first molar, and the vertical height of the optimal implantation site in males was appropriately higher than that in females.
目的为了确定迷你螺钉的最佳位置,本研究比较了成年男性和女性患者的皮质骨密度、皮质骨厚度和颧骨下嵴区域的可用骨宽度。方法收集了 200 名患者(20-30 岁,男性 100 名,女性 100 名)的锥束计算机断层扫描成像数据。将矢状面上的右上颌后牙从近端到远端分为六个层次,并在距离骨水泥8、10和12毫米的垂直距离上设置三个测量点。结果成年男性和女性患者颧骨下嵴的皮质骨密度、皮质骨厚度和可用骨宽度在上颌第二前磨牙和上颌第一磨牙之间的关节间隙水平最高。骨皮质密度和厚度随垂直高度的增加而增加,而可用骨宽度则随垂直高度的增加而减少。结论颧骨下微种植体固定的最佳种植部位是上颌第二前磨牙和上颌第一磨牙之间的垂直间隙,男性最佳种植部位的垂直高度比女性高。
{"title":"Site selection of micro-implant anchorages in the infrazygomatic crest in adult orthodontic patients.","authors":"Xinyi Chen, Xiaoge Jiang, Song Chen","doi":"10.7518/hxkq.2024.2023282","DOIUrl":"https://doi.org/10.7518/hxkq.2024.2023282","url":null,"abstract":"OBJECTIVES\u0000To determine the optimal placement of miniscrews, this study compared adult male and female patients in terms of cortical bone density, cortical bone thickness, and available bone width in the infrazygomatic crest region.\u0000\u0000\u0000METHODS\u0000The cone beam computed tomography imaging data of 200 patients (20-30 years old; 100 males and 100 females) were collected. The right maxillary posterior teeth in the sagittal plane were divided into six levels from proximal to distal, and three measurement sites were positioned at vertical distances of 8, 10, and 12 mm from the cementum. Cortical bone density, cortical bone thickness, and available bone width were measured in 18 measurement sites in the infrazygomatic crest and analyzed statistically.\u0000\u0000\u0000RESULTS\u0000The highest cortical bone density, cortical bone thickness, and available bone width in the infrazygomatic crest in adult male and female patients were at the level of the interradicular space between the maxillary second premolar and maxillary first molar. The bone cortical density and thickness increased with vertical height, whereas the available bone width decreased with increasing vertical height. Differences were observed in cortical bone density, cortical bone thickness, and available bone width between adult male and female patients.\u0000\u0000\u0000CONCLUSIONS\u0000The optimal implantation sites of the micro-implant anchorages in the infrazygomatic crest were at the level of the interradicular space between the maxillary second premolar and the maxillary first molar, and the vertical height of the optimal implantation site in males was appropriately higher than that in females.","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"51 ","pages":"207-213"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140769974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of surface nanomorphology on the senescence of periodontal ligament stem cells. 表面纳米形态对牙周韧带干细胞衰老的影响
Yanping Sun, Li Liao
OBJECTIVESThe effect of TiO2 nanotube morphology on the differentiation potency of senescent periodontal ligament stem cells was investigated.METHODSTwo types of titanium sheets with TiO2 nanotube morphology (20V-NT and 70V-NT) were prepared via anodic oxidation at 20 and 70 V separately, and their surface morphology was observed. Young periodontal ligament stem cells were cultivated in an osteogenic induction medium, and the most effective surface morphology in promoting osteogenic differentiation was selected. RO3306 and Nutlin-3a were used to induce the aging of young periodontal ligament stem cells, and senescent periodontal ligament stem cells were obtained. The osteogenic differentiation of senescent periodontal ligament stem cells was induced, and the effect of surface morphology on osteogenic differentiation was observed.RESULTSNanotube morphology was achieved on the surfaces of titanium sheets through anodic oxidation, and the diameters of the nanotubes increased with voltage. A significant difference in the effect of nanotube morphology was found among nanotubes with different diameters in the young periodontal ligament stem cells. The surface nanotube morphology of 20V-NT had a more significant effect that promoted osteogenic differentiation. Compared with a smooth titanium sheet, the surface nanotube morphology of 20V-NT increased the number of alkaline phosphatase-positive senescent periodontal ligament stem cells and promoted calcium deposition and the expression of osteogenic marker genes Runt-related transcription factor 2, osteopontin, and osteocalcin.CONCLUSIONSA special nanotube morphology enhances the differentiation ability of senescent periodontal ligament stem cells, provides an effective method for periodontal regeneration, and further improves the performance of implants.
目的研究TiO2纳米管形态对衰老牙周韧带干细胞分化效力的影响。方法分别在20V和70V电压下阳极氧化制备两种具有TiO2纳米管形态的钛片(20V-NT和70V-NT),并观察其表面形态。在成骨诱导培养基中培养年轻的牙周韧带干细胞,筛选出促进成骨分化最有效的表面形态。用RO3306和Nutlin-3a诱导年轻牙周韧带干细胞衰老,得到衰老牙周韧带干细胞。结果 通过阳极氧化在钛片表面实现了纳米管形态,纳米管的直径随电压增加而增大。在年轻的牙周韧带干细胞中,不同直径的纳米管对纳米管形态的影响存在显著差异。20V-NT的表面纳米管形态对成骨分化有更显著的促进作用。与光滑的钛片相比,20V-NT 的表面纳米管形态增加了碱性磷酸酶阳性衰老牙周韧带干细胞的数量,促进了钙沉积和成骨标志基因 Runt 相关转录因子 2、骨生成素和骨钙素的表达。
{"title":"Effects of surface nanomorphology on the senescence of periodontal ligament stem cells.","authors":"Yanping Sun, Li Liao","doi":"10.7518/hxkq.2024.2023244","DOIUrl":"https://doi.org/10.7518/hxkq.2024.2023244","url":null,"abstract":"OBJECTIVES\u0000The effect of TiO2 nanotube morphology on the differentiation potency of senescent periodontal ligament stem cells was investigated.\u0000\u0000\u0000METHODS\u0000Two types of titanium sheets with TiO2 nanotube morphology (20V-NT and 70V-NT) were prepared via anodic oxidation at 20 and 70 V separately, and their surface morphology was observed. Young periodontal ligament stem cells were cultivated in an osteogenic induction medium, and the most effective surface morphology in promoting osteogenic differentiation was selected. RO3306 and Nutlin-3a were used to induce the aging of young periodontal ligament stem cells, and senescent periodontal ligament stem cells were obtained. The osteogenic differentiation of senescent periodontal ligament stem cells was induced, and the effect of surface morphology on osteogenic differentiation was observed.\u0000\u0000\u0000RESULTS\u0000Nanotube morphology was achieved on the surfaces of titanium sheets through anodic oxidation, and the diameters of the nanotubes increased with voltage. A significant difference in the effect of nanotube morphology was found among nanotubes with different diameters in the young periodontal ligament stem cells. The surface nanotube morphology of 20V-NT had a more significant effect that promoted osteogenic differentiation. Compared with a smooth titanium sheet, the surface nanotube morphology of 20V-NT increased the number of alkaline phosphatase-positive senescent periodontal ligament stem cells and promoted calcium deposition and the expression of osteogenic marker genes Runt-related transcription factor 2, osteopontin, and osteocalcin.\u0000\u0000\u0000CONCLUSIONS\u0000A special nanotube morphology enhances the differentiation ability of senescent periodontal ligament stem cells, provides an effective method for periodontal regeneration, and further improves the performance of implants.","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"64 51","pages":"172-180"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical considerations of emergent oral manifestations during pregnancy. 孕期突发口腔表现的临床考虑因素。
Xuefeng Zhang, Xian Liu, Chang Liu, C. Hua
Pregnancy is a special period for developing and treating oral diseases. Oral emergencies during pregnancy need to be handled appropriately. Changes in the physiological environments and personal habits of pregnant women increase susceptibility to some oral diseases. However, clinical treatment strategies are limited due to the need to ensure the safety of pregnant women and fetuses. Pregnant women should obtain oral health knowledge and enhance their awareness. Dentists should adhere to the principle of "prevention before pregnancy, controlling symptoms during pregnancy, and treating diseases after pregnancy" for different pregnancy periods. They should also formulate appropriate treatment plans to control emergencies, prevent disease progression, and avoid harmful effects on pregnant women by using the safest, simplest, and most effective strategies that avoid adverse effects on fetuses. Pregnant women and dentists should combine prevention and treatment while collaborating in maintaining oral health during pregnancy. This article focuses on the principles of treatment during pregnancy, and the treatment timing, clinical management, and treatment strategies of different diseases causing oral emergencies during pregnancy are reviewed.
孕期是口腔疾病发生和治疗的特殊时期。孕期的口腔急症需要妥善处理。孕妇生理环境和个人习惯的改变会增加对某些口腔疾病的易感性。然而,由于需要确保孕妇和胎儿的安全,临床治疗策略有限。孕妇应掌握口腔保健知识,增强口腔保健意识。针对不同孕期,牙科医生应坚持 "孕前预防、孕期控制症状、孕后疾病治疗 "的原则。牙科医生应针对不同孕期,坚持 "孕前预防、孕期控制、孕后疾病治疗 "的原则,制定相应的治疗方案,以最安全、最简单、最有效的策略控制突发情况,预防疾病进展,避免对孕妇造成危害,避免对胎儿造成不良影响。孕妇和牙医应将预防和治疗结合起来,共同维护孕期口腔健康。本文重点介绍了孕期治疗原则,并对孕期引起口腔急症的不同疾病的治疗时机、临床管理和治疗策略进行了综述。
{"title":"Clinical considerations of emergent oral manifestations during pregnancy.","authors":"Xuefeng Zhang, Xian Liu, Chang Liu, C. Hua","doi":"10.7518/hxkq.2024.2023367","DOIUrl":"https://doi.org/10.7518/hxkq.2024.2023367","url":null,"abstract":"Pregnancy is a special period for developing and treating oral diseases. Oral emergencies during pregnancy need to be handled appropriately. Changes in the physiological environments and personal habits of pregnant women increase susceptibility to some oral diseases. However, clinical treatment strategies are limited due to the need to ensure the safety of pregnant women and fetuses. Pregnant women should obtain oral health knowledge and enhance their awareness. Dentists should adhere to the principle of \"prevention before pregnancy, controlling symptoms during pregnancy, and treating diseases after pregnancy\" for different pregnancy periods. They should also formulate appropriate treatment plans to control emergencies, prevent disease progression, and avoid harmful effects on pregnant women by using the safest, simplest, and most effective strategies that avoid adverse effects on fetuses. Pregnant women and dentists should combine prevention and treatment while collaborating in maintaining oral health during pregnancy. This article focuses on the principles of treatment during pregnancy, and the treatment timing, clinical management, and treatment strategies of different diseases causing oral emergencies during pregnancy are reviewed.","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"258 ","pages":"142-153"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of a diagnostic model based on random forest and artificial neural network for peri-implantitis. 构建基于随机森林和人工神经网络的种植体周围炎诊断模型。
Haoran Yang, Yuxiang Chen, Anna Zhao, Tingting Cheng, Jianzhong Zhou, Ziliang Li
OBJECTIVESThis study aimed to reveal critical genes regulating peri-implantitis during its development and construct a diagnostic model by using random forest (RF) and artificial neural network (ANN).METHODSGSE-33774, GSE106090, and GSE57631 datasets were obtained from the GEO database. The GSE33774 and GSE106090 datasets were analyzed for differential expression and functional enrichment. The protein-protein interaction networks (PPI) and RF screened vital genes. A diagnostic model for peri-implantitis was established using ANN and validated on the GSE33774 and GSE57631 datasets. A transcription factor-gene interaction network and a transcription factor-micro-RNA (miRNA) regulatory network were also established.RESULTSA total of 124 differentially expressed genes (DEGs) involved in the regulation of peri-implantitis were screened. Enrichment analysis showed that DEGs were mainly associated with immune receptor activity and cytokine receptor activity and were mainly involved in processes such as leukocyte and neutrophil migration. The PPI and RF screened six essential genes, namely, CD38, CYBB, FCGR2A, SELL, TLR4, and CXCL8. The receiver operating characteristic curve (ROC) indicated that the ANN model had an excellent diagnostic performance. FOXC1, GATA2, and NF-κB1 may be essential transcription factors in peri-implantitis, and hsa-miR-204 may be a key miRNA.CONCLUSIONSThe diagnostic model of peri-implantitis constructed by RF and ANN has high confidence, and CD38, CYBB, FCGR2A, SELL, TLR4, and CXCL8 are potential diagnostic markers. FOXC1, GATA2, and NF-κB1 may be essential transcription factors in peri-implantitis, and hsa-miR-204 plays a vital role as a critical miRNA.
目的:本研究旨在揭示冠周炎发生过程中调控冠周炎的关键基因,并利用随机森林(RF)和人工神经网络(ANN)构建诊断模型。对 GSE33774 和 GSE106090 数据集进行了差异表达和功能富集分析。蛋白质-蛋白质相互作用网络(PPI)和RF筛选了重要基因。利用 ANN 建立了种植体周围炎的诊断模型,并在 GSE33774 和 GSE57631 数据集上进行了验证。结果共筛选出 124 个参与调控种植体周围炎的差异表达基因(DEGs)。富集分析表明,DEGs 主要与免疫受体活性和细胞因子受体活性有关,并主要参与白细胞和中性粒细胞迁移等过程。PPI 和 RF 筛选出了 6 个重要基因,即 CD38、CYBB、FCGR2A、SELL、TLR4 和 CXCL8。接受者操作特征曲线(ROC)表明,ANN 模型具有出色的诊断性能。FOXC1、GATA2和NF-κB1可能是种植体周围炎的重要转录因子,而hsa-miR-204可能是关键的miRNA。结论通过RF和ANN构建的种植体周围炎诊断模型具有很高的可信度,CD38、CYBB、FCGR2A、SELL、TLR4和CXCL8是潜在的诊断标志物。FOXC1、GATA2和NF-κB1可能是种植体周围炎的重要转录因子,而hsa-miR-204作为关键的miRNA起着至关重要的作用。
{"title":"Construction of a diagnostic model based on random forest and artificial neural network for peri-implantitis.","authors":"Haoran Yang, Yuxiang Chen, Anna Zhao, Tingting Cheng, Jianzhong Zhou, Ziliang Li","doi":"10.7518/hxkq.2024.2023275","DOIUrl":"https://doi.org/10.7518/hxkq.2024.2023275","url":null,"abstract":"OBJECTIVES\u0000This study aimed to reveal critical genes regulating peri-implantitis during its development and construct a diagnostic model by using random forest (RF) and artificial neural network (ANN).\u0000\u0000\u0000METHODS\u0000GSE-33774, GSE106090, and GSE57631 datasets were obtained from the GEO database. The GSE33774 and GSE106090 datasets were analyzed for differential expression and functional enrichment. The protein-protein interaction networks (PPI) and RF screened vital genes. A diagnostic model for peri-implantitis was established using ANN and validated on the GSE33774 and GSE57631 datasets. A transcription factor-gene interaction network and a transcription factor-micro-RNA (miRNA) regulatory network were also established.\u0000\u0000\u0000RESULTS\u0000A total of 124 differentially expressed genes (DEGs) involved in the regulation of peri-implantitis were screened. Enrichment analysis showed that DEGs were mainly associated with immune receptor activity and cytokine receptor activity and were mainly involved in processes such as leukocyte and neutrophil migration. The PPI and RF screened six essential genes, namely, CD38, CYBB, FCGR2A, SELL, TLR4, and CXCL8. The receiver operating characteristic curve (ROC) indicated that the ANN model had an excellent diagnostic performance. FOXC1, GATA2, and NF-κB1 may be essential transcription factors in peri-implantitis, and hsa-miR-204 may be a key miRNA.\u0000\u0000\u0000CONCLUSIONS\u0000The diagnostic model of peri-implantitis constructed by RF and ANN has high confidence, and CD38, CYBB, FCGR2A, SELL, TLR4, and CXCL8 are potential diagnostic markers. FOXC1, GATA2, and NF-κB1 may be essential transcription factors in peri-implantitis, and hsa-miR-204 plays a vital role as a critical miRNA.","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"433 ","pages":"214-226"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Verification of the expression trend and interaction prediction of innate immune cells and immune-checkpoint molecules in the process of oral mucosal carcinogenesis. 验证先天性免疫细胞和免疫检查点分子在口腔黏膜癌变过程中的表达趋势和相互作用预测。
Kaiyu Li, Lijuan Shi, Linxin Liu, Jie Wang, Minhai Nie, Xuqian Liu
OBJECTIVESThis study aimed to explore the expression trends of innate immune cells and immune-checkpoint molecules validated by data calculation in the process of oral mucosal carcinogenesis, as well as to explore methods of suppressing oral mucosal carcinogenesis based on immunotherapy by predicting their interactions. Me-thods 1) The cancer genome atlas (TCGA) database comprehensively scores immune cells and immune-checkpoint molecules in the process of oral mucosal carcinogenesis and screens out intrinsic immune cells and immune-checkpoint molecules that interfere with tumor immune escape. 2) Clinical patient blood routine data were collected for the statistical analysis of peripheral blood immune cells during the progression of oral mucosal carcinogenesis. Immune cells in peripheral blood that may affect the progression of oral mucosal carcinogenesis were screened. 3) Immunohistochemical staining was performed on intrinsic immune cells and immune-checkpoint molecules validated based on data calculation in various stages of oral mucosal carcinogenesis. 4) Special staining was used to identify innate immune cells in various stages of oral mucosal carcinogenesis based on data-calculation verification. 5) Survival analysis was conducted on intrinsic immune cells and immune-checkpoint molecules validated based on data calculation during the process of oral mucosal carcinogenesis. The association of intrinsic immune cells and immune-checkpoint molecules with the prognosis of oral squamous cell carcinoma was verified.RESULTSThe expression of monocytes and neutrophils increased during the process of oral mucosal carcinogenesis. The expression of eosinophils showed a single peak trend of up and down. The expression of mast cells decreased. In the process of oral mucosal carcinogenesis, the expression of the immune-checkpoint molecules cytotoxic T-lymphocyte-associated protein 4 (CTLA4) and programmed cell death-ligand (PD-L1) increased. The expression trends of monocytes, neutrophils, and eosinophils were positively correlated with those of CTLA4 and PD-L1 immune-checkpoint molecules. The expression trend of mast cells was negatively correlated with the expression of CTLA4 and PD-L1. Monocytes, neutrophils, and eosinophils may promote tumor immune escape mediated by CTLA4 and/or PD-L1, thereby accelerating the progression of oral mucosal carcinogenesis. Mast cells may inhibit tumor immune escape mediated by CTLA4 and/or PD-L1, delaying the progression of oral mucosal carcinogenesis.CONCLUSIONSTherefore, interference with specific immune cells in innate immunity can regulate the expression of CTLA4 and/or PD-L1 to a certain extent, inhibit tumor immune escape, and delay the progression of oral mucosal carcinogenesis.
目的 本研究旨在通过数据计算验证先天性免疫细胞和免疫检查点分子在口腔黏膜癌变过程中的表达趋势,并通过预测它们之间的相互作用,探索基于免疫疗法抑制口腔黏膜癌变的方法。方法 1)癌症基因组图谱(TCGA)数据库对口腔黏膜癌发生过程中的免疫细胞和免疫检查点分子进行全面评分,筛选出干扰肿瘤免疫逃逸的固有免疫细胞和免疫检查点分子。2) 收集临床患者血常规数据,对口腔黏膜癌发生过程中的外周血免疫细胞进行统计分析。筛选外周血中可能影响口腔黏膜癌发生的免疫细胞。3) 对口腔黏膜癌变不同阶段的固有免疫细胞和根据数据计算验证的免疫检查点分子进行免疫组化染色。4) 根据数据计算验证,在口腔黏膜癌发生的不同阶段采用特殊染色法鉴定先天性免疫细胞。5) 对口腔黏膜癌变过程中的固有免疫细胞和基于数据计算验证的免疫检查点分子进行生存分析。结果口腔黏膜癌发生过程中,单核细胞和中性粒细胞表达增加。嗜酸性粒细胞的表达呈单峰上升和下降趋势。肥大细胞的表达量减少。在口腔黏膜癌发生过程中,免疫检查点分子细胞毒性 T 淋巴细胞相关蛋白 4(CTLA4)和程序性细胞死亡配体(PD-L1)的表达增加。单核细胞、中性粒细胞和嗜酸性粒细胞的表达趋势与 CTLA4 和 PD-L1 免疫检查点分子的表达趋势呈正相关。肥大细胞的表达趋势与 CTLA4 和 PD-L1 的表达呈负相关。单核细胞、中性粒细胞和嗜酸性粒细胞可能会促进由 CTLA4 和/或 PD-L1 介导的肿瘤免疫逃逸,从而加速口腔黏膜癌的进展。因此,干扰先天性免疫中的特异性免疫细胞可在一定程度上调节 CTLA4 和/或 PD-L1 的表达,抑制肿瘤免疫逃逸,延缓口腔黏膜癌的发生。
{"title":"Verification of the expression trend and interaction prediction of innate immune cells and immune-checkpoint molecules in the process of oral mucosal carcinogenesis.","authors":"Kaiyu Li, Lijuan Shi, Linxin Liu, Jie Wang, Minhai Nie, Xuqian Liu","doi":"10.7518/hxkq.2024.2023280","DOIUrl":"https://doi.org/10.7518/hxkq.2024.2023280","url":null,"abstract":"OBJECTIVES\u0000This study aimed to explore the expression trends of innate immune cells and immune-checkpoint molecules validated by data calculation in the process of oral mucosal carcinogenesis, as well as to explore methods of suppressing oral mucosal carcinogenesis based on immunotherapy by predicting their interactions. Me-thods 1) The cancer genome atlas (TCGA) database comprehensively scores immune cells and immune-checkpoint molecules in the process of oral mucosal carcinogenesis and screens out intrinsic immune cells and immune-checkpoint molecules that interfere with tumor immune escape. 2) Clinical patient blood routine data were collected for the statistical analysis of peripheral blood immune cells during the progression of oral mucosal carcinogenesis. Immune cells in peripheral blood that may affect the progression of oral mucosal carcinogenesis were screened. 3) Immunohistochemical staining was performed on intrinsic immune cells and immune-checkpoint molecules validated based on data calculation in various stages of oral mucosal carcinogenesis. 4) Special staining was used to identify innate immune cells in various stages of oral mucosal carcinogenesis based on data-calculation verification. 5) Survival analysis was conducted on intrinsic immune cells and immune-checkpoint molecules validated based on data calculation during the process of oral mucosal carcinogenesis. The association of intrinsic immune cells and immune-checkpoint molecules with the prognosis of oral squamous cell carcinoma was verified.\u0000\u0000\u0000RESULTS\u0000The expression of monocytes and neutrophils increased during the process of oral mucosal carcinogenesis. The expression of eosinophils showed a single peak trend of up and down. The expression of mast cells decreased. In the process of oral mucosal carcinogenesis, the expression of the immune-checkpoint molecules cytotoxic T-lymphocyte-associated protein 4 (CTLA4) and programmed cell death-ligand (PD-L1) increased. The expression trends of monocytes, neutrophils, and eosinophils were positively correlated with those of CTLA4 and PD-L1 immune-checkpoint molecules. The expression trend of mast cells was negatively correlated with the expression of CTLA4 and PD-L1. Monocytes, neutrophils, and eosinophils may promote tumor immune escape mediated by CTLA4 and/or PD-L1, thereby accelerating the progression of oral mucosal carcinogenesis. Mast cells may inhibit tumor immune escape mediated by CTLA4 and/or PD-L1, delaying the progression of oral mucosal carcinogenesis.\u0000\u0000\u0000CONCLUSIONS\u0000Therefore, interference with specific immune cells in innate immunity can regulate the expression of CTLA4 and/or PD-L1 to a certain extent, inhibit tumor immune escape, and delay the progression of oral mucosal carcinogenesis.","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"216 ","pages":"192-206"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140786101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of circadian clock protein Bmal1 on experimentally-induced periodontitis-associated renal injury. 昼夜节律时钟蛋白 Bmal1 对实验性牙周炎相关肾损伤的影响
Haonan Ma, Qiong Li, Yaqi Shang, Xirui Xin, Xinchan Liu, Zhou Wu, Weixian Yu
OBJECTIVESTo investigate the mechanism of circadian clock protein Bmal1 (Bmal1) on renal injury with chronic periodontitis, we established an experimental rat periodontitis model.METHODSTwelve male Wistar rats were randomly divided into control and periodontitis groups (n=6, each group). The first maxillary molars on both sides of the upper jaw of rats with periodontitis were ligated by using orthodontic ligature wires, whereas the control group received no intervention measures. After 8 weeks, clinical periodontal parameters, including probing depth, bleeding index, and tooth mobility, were evaluated in both groups. Micro-CT scanning and three-dimensional image reconstruction were performed on the maxillary bones of the rats for the assessment of alveolar bone resorption. Histopatholo-gical observations of periodontal and renal tissues were conducted using hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining. Renal function indicators, such as creatinine, albumin, and blood urea nitrogen levels, and oxidative stress markers, including superoxide dismutase, glutathione, and malondialdehyde levels, were measured using biochemical assay kits. MitoSOX red staining was used to detect reactive oxygen species (ROS) content in the kidneys. The gene and protein expression levels of Bmal1, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in rat renal tissues were assessed using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical staining.RESULTSMicro-CT and HE staining results showed significant bone resorption and attachment loss in the maxillary first molar region of the periodontitis group. Histological examination through HE and PAS staining revealed substantial histopathological damage to the renal tissues of the rats in the periodontitis group. The findings of the assessment of renal function and oxidative stress markers indicated that the periodontitis group exhibited abnormal levels of oxidative stress, whereas the renal function levels showed abnormalities without statistical significance. MitoSOX Red staining results showed that the content of ROS in the renal tissue of the periodontitis group was significantly higher than that of the control group, and RT-qPCR and immunohistochemistry results showed that the expression levels of Bmal1, Nrf2, and HO-1 in the renal tissues of the rats in the periodontitis group showed a decreasing trend.CONCLUSIONSCircadian clock protein Bmal1 plays an important role in the oxidative damage process involved in the renal of rats with periodontitis.
方法将12只雄性Wistar大鼠随机分为对照组和牙周炎组(每组6只)。用正畸结扎线结扎牙周炎大鼠上颌两侧的第一颗上颌磨牙,而对照组不采取任何干预措施。8 周后,评估两组大鼠的临床牙周参数,包括探诊深度、出血指数和牙齿活动度。对大鼠的上颌骨进行显微 CT 扫描和三维图像重建,以评估牙槽骨吸收情况。使用苏木精-伊红(HE)和周期性酸-希夫(PAS)染色法对牙周组织和肾组织进行组织病理学观察。肾功能指标(如肌酐、白蛋白和血尿素氮水平)和氧化应激指标(包括超氧化物歧化酶、谷胱甘肽和丙二醛水平)均采用生化检测试剂盒进行测量。MitoSOX 红染色用于检测肾脏中活性氧(ROS)的含量。使用实时定量聚合酶链反应(RT-qPCR)和免疫组化染色法评估了大鼠肾组织中 Bmal1、核因子红细胞 2 相关因子 2(Nrf2)和血红素加氧酶 1(HO-1)的基因和蛋白表达水平。通过 HE 和 PAS 染色进行的组织学检查显示,牙周炎组大鼠的肾组织受到了严重的组织病理学损伤。肾功能和氧化应激标记物的评估结果表明,牙周炎组的氧化应激水平异常,而肾功能水平出现异常,但无统计学意义。MitoSOX红染色结果显示,牙周炎组大鼠肾组织中ROS的含量明显高于对照组,RT-qPCR和免疫组化结果显示,牙周炎组大鼠肾组织中Bmal1、Nrf2和HO-1的表达水平呈下降趋势。
{"title":"Impact of circadian clock protein Bmal1 on experimentally-induced periodontitis-associated renal injury.","authors":"Haonan Ma, Qiong Li, Yaqi Shang, Xirui Xin, Xinchan Liu, Zhou Wu, Weixian Yu","doi":"10.7518/hxkq.2024.2023245","DOIUrl":"https://doi.org/10.7518/hxkq.2024.2023245","url":null,"abstract":"OBJECTIVES\u0000To investigate the mechanism of circadian clock protein Bmal1 (Bmal1) on renal injury with chronic periodontitis, we established an experimental rat periodontitis model.\u0000\u0000\u0000METHODS\u0000Twelve male Wistar rats were randomly divided into control and periodontitis groups (n=6, each group). The first maxillary molars on both sides of the upper jaw of rats with periodontitis were ligated by using orthodontic ligature wires, whereas the control group received no intervention measures. After 8 weeks, clinical periodontal parameters, including probing depth, bleeding index, and tooth mobility, were evaluated in both groups. Micro-CT scanning and three-dimensional image reconstruction were performed on the maxillary bones of the rats for the assessment of alveolar bone resorption. Histopatholo-gical observations of periodontal and renal tissues were conducted using hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining. Renal function indicators, such as creatinine, albumin, and blood urea nitrogen levels, and oxidative stress markers, including superoxide dismutase, glutathione, and malondialdehyde levels, were measured using biochemical assay kits. MitoSOX red staining was used to detect reactive oxygen species (ROS) content in the kidneys. The gene and protein expression levels of Bmal1, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in rat renal tissues were assessed using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical staining.\u0000\u0000\u0000RESULTS\u0000Micro-CT and HE staining results showed significant bone resorption and attachment loss in the maxillary first molar region of the periodontitis group. Histological examination through HE and PAS staining revealed substantial histopathological damage to the renal tissues of the rats in the periodontitis group. The findings of the assessment of renal function and oxidative stress markers indicated that the periodontitis group exhibited abnormal levels of oxidative stress, whereas the renal function levels showed abnormalities without statistical significance. MitoSOX Red staining results showed that the content of ROS in the renal tissue of the periodontitis group was significantly higher than that of the control group, and RT-qPCR and immunohistochemistry results showed that the expression levels of Bmal1, Nrf2, and HO-1 in the renal tissues of the rats in the periodontitis group showed a decreasing trend.\u0000\u0000\u0000CONCLUSIONS\u0000Circadian clock protein Bmal1 plays an important role in the oxidative damage process involved in the renal of rats with periodontitis.","PeriodicalId":94028,"journal":{"name":"Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology","volume":"12 3","pages":"163-171"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140786293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A retrospective study on iRoot BP Plus full pulpotomy for primary molars with partial irreversible pulpitis. 关于 iRoot BP Plus 全牙髓切断术治疗部分不可逆牙髓炎的回顾性研究。
Xiaoyan Hu, Chunhui Zhao, Lu Wang, Zheng Zhang, Fan Yang, Hongyan Zhang
OBJECTIVESThis study aimed to observe the outcomes of iRoot BP Plus full pulpotomy in primary molars with partial irreversible pulpitis retrospectively.METHODSCollect 102 cases of primary molars with partial irreversible pulpitis undergoing iRoot BP Plus full pulpotomy from January 2019 to August 2023, with a follow-up period of 24-47 months. Based on the presence of irreversible pulpitis symptoms before surgery, the included cases will be divided into asymptomatic group (n=53) and symptomatic group (n=49). Observe the clinical and imaging success rates of both groups.RESULTSClinical success rates were 96.2% and 97.9% in asymptomatic and symptomatic groups, and radiographic success rates were 96.2% and 93.9% respectively.CONCLUSIONSiRoot BP Plus full pulpotomy can be used for the treatment of primary molars with partial irreversible pulpitis under an enhanced pulpotomy protocol.
目的本研究旨在回顾性观察iRoot BP Plus全牙髓切断术在部分不可逆牙髓炎的初级磨牙中的疗效。方法收集2019年1月至2023年8月期间接受iRoot BP Plus全牙髓切断术的部分不可逆牙髓炎初级磨牙102例,随访24-47个月。根据术前是否存在不可逆牙髓炎症状,将纳入病例分为无症状组(n=53)和有症状组(n=49)。结果无症状组和有症状组的临床成功率分别为96.2%和97.9%,放射学成功率分别为96.2%和93.9%。结论在增强型牙髓切断术方案下,iRoot BP Plus全牙髓切断术可用于治疗部分不可逆牙髓炎的初级磨牙。
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Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
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