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A New Era of Data-Driven Cancer Research and Care: Opportunities and Challenges. 数据驱动的癌症研究与护理新时代:机遇与挑战。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-24-1130
Felicia Gomez, Arpad M Danos, Guilherme Del Fiol, Anant Madabhushi, Pallavi Tiwari, Joshua F McMichael, Spyridon Bakas, Jiang Bian, Christos Davatzikos, Elana J Fertig, Jayashree Kalpathy-Cramer, Johanna Kenney, Guergana K Savova, Meliha Yetisgen, Eliezer M Van Allen, Jeremy L Warner, Fred Prior, Malachi Griffith, Obi L Griffith

People diagnosed with cancer and their formal and informal caregivers are increasingly faced with a deluge of complex information, thanks to rapid advancements in the type and volume of diagnostic, prognostic, and treatment data. This commentary discusses the opportunities and challenges that the society faces as we integrate large volumes of data into regular cancer care.

由于诊断、预后和治疗数据的类型和数量突飞猛进,被诊断为癌症的患者及其正式和非正式的护理人员越来越多地面临着大量复杂的信息。这篇评论探讨了当我们将大量数据整合到常规癌症护理中时,社会所面临的机遇和挑战。
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引用次数: 0
Iron Chelation Therapy Elicits Innate Immune Control of Metastatic Ovarian Cancer. 铁螯合疗法可激发对转移性卵巢癌的先天性免疫控制
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-23-1451
Tito A Sandoval, Camilla Salvagno, Chang-Suk Chae, Deepika Awasthi, Paolo Giovanelli, Matias Marin Falco, Sung-Min Hwang, Eli Teran-Cabanillas, Lasse Suominen, Takahiro Yamazaki, Hui-Hsuan Kuo, Jenna E Moyer, M Laura Martin, Jyothi Manohar, Kihwan Kim, Maria A Sierra, Yusibeska Ramos, Chen Tan, Alexander Emmanuelli, Minkyung Song, Diana K Morales, Dmitriy Zamarin, Melissa K Frey, Evelyn Cantillo, Eloise Chapman-Davis, Kevin Holcomb, Christopher E Mason, Lorenzo Galluzzi, Zhen Ni Zhou, Anna Vähärautio, Suzanne M Cloonan, Juan R Cubillos-Ruiz

Iron accumulation in tumors contributes to disease progression and chemoresistance. Although targeting this process can influence various hallmarks of cancer, the immunomodulatory effects of iron chelation in the tumor microenvironment are unknown. Here, we report that treatment with deferiprone, an FDA-approved iron chelator, unleashes innate immune responses that restrain ovarian cancer. Deferiprone reprogrammed ovarian cancer cells toward an immunostimulatory state characterized by the production of type-I IFN and overexpression of molecules that activate NK cells. Mechanistically, these effects were driven by innate sensing of mitochondrial DNA in the cytosol and concomitant activation of nuclear DNA damage responses triggered upon iron chelation. Deferiprone synergized with chemotherapy and prolonged the survival of mice with ovarian cancer by bolstering type-I IFN responses that drove NK cell-dependent control of metastatic disease. Hence, iron chelation may represent an alternative immunotherapeutic strategy for malignancies that are refractory to current T-cell-centric modalities. Significance: This study uncovers that targeting dysregulated iron accumulation in ovarian tumors represents a major therapeutic opportunity. Iron chelation therapy using an FDA-approved agent causes immunogenic stress responses in ovarian cancer cells that delay metastatic disease progression and enhance the effects of first-line chemotherapy. See related commentary by Bell and Zou, p. 1771.

肿瘤中的铁积累会导致疾病进展和化疗耐药性。虽然针对这一过程可以影响癌症的各种特征,但铁螯合在肿瘤微环境中的免疫调节作用尚不清楚。在这里,我们报告了使用美国食品及药物管理局批准的铁螯合剂去铁酮治疗可释放抑制卵巢癌的先天性免疫反应。去铁酮能使卵巢癌细胞重新编程,进入以产生 I 型干扰素(IFN)和过表达激活自然杀伤(NK)细胞的分子为特征的免疫刺激状态。从机理上讲,这些效应是由细胞质中线粒体 DNA 的先天感应和铁螯合引发的核 DNA 损伤反应同时激活所驱动的。去铁酮能与化疗产生协同作用,并通过增强 I 型 IFN 反应延长卵巢癌小鼠的生存期,而 I 型 IFN 反应又能促进 NK 细胞对转移性疾病的依赖性控制。因此,对于目前以 T 细胞为中心的治疗方式难以奏效的恶性肿瘤,螯合铁可能是另一种免疫治疗策略。
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引用次数: 0
The Epigenetic Hallmarks of Cancer. 癌症的表观遗传特征
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-24-0296
Manel Esteller, Mark A Dawson, Cigall Kadoch, Feyruz V Rassool, Peter A Jones, Stephen B Baylin

Cancer is a complex disease in which several molecular and cellular pathways converge to foster the tumoral phenotype. Notably, in the latest iteration of the cancer hallmarks, "nonmutational epigenetic reprogramming" was newly added. However, epigenetics, much like genetics, is a broad scientific area that deserves further attention due to its multiple roles in cancer initiation, progression, and adaptive nature. Herein, we present a detailed examination of the epigenetic hallmarks affected in human cancer, elucidating the pathways and genes involved, and dissecting the disrupted landscapes for DNA methylation, histone modifications, and chromatin architecture that define the disease. Significance: Cancer is a disease characterized by constant evolution, spanning from its initial premalignant stages to the advanced invasive and disseminated stages. It is a pathology that is able to adapt and survive amidst hostile cellular microenvironments and diverse treatments implemented by medical professionals. The more fixed setup of the genetic structure cannot fully provide transformed cells with the tools to survive but the rapid and plastic nature of epigenetic changes is ready for the task. This review summarizes the epigenetic hallmarks that define the ecological success of cancer cells in our bodies.

癌症是一种复杂的疾病,在这种疾病中,多种分子和细胞途径汇聚在一起,形成了肿瘤表型。值得注意的是,在最新迭代的癌症标志中,新增了 "非突变表观遗传重编程"。然而,与遗传学一样,表观遗传学也是一个广泛的科学领域,由于其在癌症发生、发展和适应性方面的多重作用,值得进一步关注。在本文中,我们将对人类癌症中受影响的表观遗传学特征进行详细研究,阐明其中涉及的途径和基因,并剖析确定疾病的 DNA 甲基化、组蛋白修饰和染色质结构的破坏性景观。意义重大:癌症是一种以不断演变为特征的疾病,从最初的恶性肿瘤前期发展到晚期的浸润和扩散阶段。它是一种能够在恶劣的细胞微环境和医学专家实施的各种治疗方法中适应和生存的病理学。较为固定的遗传结构设置无法为转化细胞提供完全的生存工具,但表观遗传变化的快速和可塑性已为这一任务做好了准备。本综述总结了决定癌细胞在人体内生态成功的表观遗传学标志。
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引用次数: 0
Transforming Cancer Research through Informatics. 通过信息学改变癌症研究。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-24-0604
Juli D Klemm, Dinah S Singer, Jill P Mesirov

For more than three decades, concurrent advances in laboratory technologies and computer science have driven the rise of cancer informatics. Today, software tools for cancer research are indispensable to the entire cancer research enterprise.

三十多年来,实验室技术和计算机科学的同步发展推动了癌症信息学的兴起。如今,用于癌症研究的软件工具已成为整个癌症研究领域不可或缺的工具。
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引用次数: 0
Perivascular NOTCH3+ Stem Cells Drive Meningioma Tumorigenesis and Resistance to Radiotherapy. 血管周围NOTCH3+干细胞驱动脑膜瘤肿瘤发生并对放疗产生抗药性。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-23-1459
Abrar Choudhury, Martha A Cady, Calixto-Hope G Lucas, Hinda Najem, Joanna J Phillips, Brisa Palikuqi, Naomi Zakimi, Tara Joseph, Janeth O Birrueta, William C Chen, Nancy A Oberheim Bush, Shawn L Hervey-Jumper, Ophir D Klein, Christine M Toedebusch, Craig M Horbinski, Stephen T Magill, Aparna Bhaduri, Arie Perry, Peter J Dickinson, Amy B Heimberger, Alan Ashworth, Elizabeth E Crouch, David R Raleigh

Meningiomas are the most common primary intracranial tumors. Treatments for patients with meningiomas are limited to surgery and radiotherapy, and systemic therapies remain ineffective or experimental. Resistance to radiotherapy is common in high-grade meningiomas and the cell types and signaling mechanisms that drive meningioma tumorigenesis and resistance to radiotherapy are incompletely understood. Here, we report that NOTCH3 drives meningioma tumorigenesis and resistance to radiotherapy and find that perivascular NOTCH3+ stem cells are conserved across meningiomas from humans, dogs, and mice. Integrating single-cell transcriptomics with lineage tracing and imaging approaches in genetically engineered mouse models and xenografts, we show NOTCH3 drives tumor-initiating capacity, cell proliferation, angiogenesis, and resistance to radiotherapy to increase meningioma growth and reduce survival. To translate these findings to patients, we show that an antibody stabilizing the extracellular negative regulatory region of NOTCH3 blocks meningioma tumorigenesis and sensitizes meningiomas to radiotherapy, reducing tumor growth and improving survival. Significance: There are no effective systemic therapies to treat meningiomas, and meningioma stem cells are poorly understood. Here, we report perivascular NOTCH3+ stem cells to drive meningioma tumorigenesis and resistance to radiotherapy. Our results identify a conserved mechanism and a therapeutic vulnerability to treat meningiomas that are resistant to standard interventions.

脑膜瘤是最常见的原发性颅内肿瘤。对脑膜瘤患者的治疗仅限于手术和放射治疗,全身治疗仍然无效或处于试验阶段。高分化脑膜瘤普遍存在放疗耐药性,而驱动脑膜瘤肿瘤发生和放疗耐药性的细胞类型和信号机制尚不完全清楚。在这里,我们报告了NOTCH3驱动脑膜瘤肿瘤发生和对放疗的耐受性,并发现血管周围NOTCH3+干细胞在人类、狗和小鼠的脑膜瘤中是保守的。在基因工程小鼠模型和异种移植物中,我们将单细胞转录组学与系谱追踪和成像方法相结合,结果表明NOTCH3驱动肿瘤的始发能力、细胞增殖、血管生成和对放疗的耐受性,从而增加脑膜瘤的生长并降低存活率。为了将这些发现应用到患者身上,我们证明了稳定 NOTCH3 细胞外负调控区的抗体可以阻止脑膜瘤的肿瘤发生,并使脑膜瘤对放疗敏感,从而减少肿瘤生长,提高生存率。
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引用次数: 0
Cancer Genomes Sometimes Take the Longest Evolutionary Road. 癌症基因组有时走最长的进化之路
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-24-1017
Alison M Taylor

Baker and colleagues developed a new algorithm called "Gain Route Identification and Timing In Cancer" (GRITIC) to uncover the path of chromosomal evolution in a tumor, particularly in the context of whole-genome duplication. Their approach found that tumors with genome doubling frequently take an indirect path from one copy number state to another. In addition, the timing of genome doubling within a tumor's evolution impacts its consequences on downstream chromosomal instability. See related article by Baker et al., p. 1810.

贝克及其同事开发了一种名为 "癌症增益路径识别与时间"(GRITIC)的新算法,用于揭示肿瘤中染色体的进化路径,尤其是在全基因组复制的情况下。他们的方法发现,出现基因组倍增的肿瘤经常从一种拷贝数状态间接进入另一种拷贝数状态。此外,肿瘤进化过程中基因组加倍的时间也会影响其对下游染色体不稳定性的影响。参见 Baker 等人的相关文章,第 1810 页。
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引用次数: 0
Clonal Lineage Tracing with Somatic Delivery of Recordable Barcodes Reveals Migration Histories of Metastatic Prostate Cancer. 通过体细胞传递可记录条形码进行克隆系谱追踪,揭示转移性前列腺癌的迁移历史。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-23-1332
Ryan N Serio, Armin Scheben, Billy Lu, Domenic V Gargiulo, Lucrezia Patruno, Caroline L Buckholtz, Ryan J Chaffee, Megan C Jibilian, Steven G Persaud, Stephen J Staklinski, Rebecca Hassett, Lise M Brault, Daniele Ramazzotti, Christopher E Barbieri, Adam C Siepel, Dawid G Nowak

The patterns by which primary tumors spread to metastatic sites remain poorly understood. Here, we define patterns of metastatic seeding in prostate cancer using a novel injection-based mouse model-EvoCaP (Evolution in Cancer of the Prostate), featuring aggressive metastatic cancer to bone, liver, lungs, and lymph nodes. To define migration histories between primary and metastatic sites, we used our EvoTraceR pipeline to track distinct tumor clones containing recordable barcodes. We detected widespread intratumoral heterogeneity from the primary tumor in metastatic seeding, with few clonal populations instigating most migration. Metastasis-to-metastasis seeding was uncommon, as most cells remained confined within the tissue. Migration patterns in our model were congruent with human prostate cancer seeding topologies. Our findings support the view of metastatic prostate cancer as a systemic disease driven by waves of aggressive clones expanding their niche, infrequently overcoming constraints that otherwise keep them confined in the primary or metastatic site. Significance: Defining the kinetics of prostate cancer metastasis is critical for developing novel therapeutic strategies. This study uses CRISPR/Cas9-based barcoding technology to accurately define tumor clonal patterns and routes of migration in a novel somatically engineered mouse model (EvoCaP) that recapitulates human prostate cancer using an in-house developed analytical pipeline (EvoTraceR).

人们对原发性肿瘤向转移部位扩散的模式仍然知之甚少。在这里,我们利用一种新型注射小鼠模型--EvoCaP(前列腺癌进化),确定了前列腺癌(PCa)的转移播种模式,该模型的特点是侵袭性癌症向骨、肝、肺和淋巴结转移。为了确定原发部位和转移部位之间的迁移历史,我们使用 EvoTraceR 管道追踪含有可记录条形码的不同肿瘤克隆。我们在转移播种中检测到了原发肿瘤广泛的瘤内异质性,少数克隆群(CP)引发了大部分迁移。转移瘤之间的播种并不常见,因为大多数细胞仍局限在组织内。我们模型中的迁移模式与人类 PCa 的播种拓扑结构一致。我们的研究结果支持这样一种观点,即转移性 PCa 是一种由侵袭性克隆波驱动的系统性疾病,它们不断扩大自己的生态位,很少能克服使它们局限在原发或转移部位的限制因素。
{"title":"Clonal Lineage Tracing with Somatic Delivery of Recordable Barcodes Reveals Migration Histories of Metastatic Prostate Cancer.","authors":"Ryan N Serio, Armin Scheben, Billy Lu, Domenic V Gargiulo, Lucrezia Patruno, Caroline L Buckholtz, Ryan J Chaffee, Megan C Jibilian, Steven G Persaud, Stephen J Staklinski, Rebecca Hassett, Lise M Brault, Daniele Ramazzotti, Christopher E Barbieri, Adam C Siepel, Dawid G Nowak","doi":"10.1158/2159-8290.CD-23-1332","DOIUrl":"10.1158/2159-8290.CD-23-1332","url":null,"abstract":"<p><p>The patterns by which primary tumors spread to metastatic sites remain poorly understood. Here, we define patterns of metastatic seeding in prostate cancer using a novel injection-based mouse model-EvoCaP (Evolution in Cancer of the Prostate), featuring aggressive metastatic cancer to bone, liver, lungs, and lymph nodes. To define migration histories between primary and metastatic sites, we used our EvoTraceR pipeline to track distinct tumor clones containing recordable barcodes. We detected widespread intratumoral heterogeneity from the primary tumor in metastatic seeding, with few clonal populations instigating most migration. Metastasis-to-metastasis seeding was uncommon, as most cells remained confined within the tissue. Migration patterns in our model were congruent with human prostate cancer seeding topologies. Our findings support the view of metastatic prostate cancer as a systemic disease driven by waves of aggressive clones expanding their niche, infrequently overcoming constraints that otherwise keep them confined in the primary or metastatic site. Significance: Defining the kinetics of prostate cancer metastasis is critical for developing novel therapeutic strategies. This study uses CRISPR/Cas9-based barcoding technology to accurately define tumor clonal patterns and routes of migration in a novel somatically engineered mouse model (EvoCaP) that recapitulates human prostate cancer using an in-house developed analytical pipeline (EvoTraceR).</p>","PeriodicalId":9430,"journal":{"name":"Cancer discovery","volume":" ","pages":"1990-2009"},"PeriodicalIF":29.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CD28 Costimulation Augments CAR Signaling in NK Cells via the LCK/CD3ζ/ZAP70 Signaling Axis. CD28 成本刺激可通过 LCK/CD3Z/ZAP70 信号轴增强 NK 细胞中的 CAR 信号。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-24-0096
Sunil Acharya, Rafet Basar, May Daher, Hind Rafei, Ping Li, Nadima Uprety, Emily Ensley, Mayra Shanley, Bijender Kumar, Pinaki P Banerjee, Luciana Melo Garcia, Paul Lin, Vakul Mohanty, Kun H Kim, Xianli Jiang, Yuchen Pan, Ye Li, Bin Liu, Ana K Nunez Cortes, Chenyu Zhang, Mohsen Fathi, Ali Rezvan, Melisa J Montalvo, Sophia L Cha, Francia Reyes-Silva, Rejeena Shrestha, Xingliang Guo, Kiran Kundu, Alexander Biederstädt, Luis Muniz-Feliciano, Gary M Deyter, Mecit Kaplan, Xin R Jiang, Enli Liu, Antrix Jain, Janos Roszik, Natalie W Fowlkes, Luisa M Solis Soto, Maria G Raso, Joseph D Khoury, Pei Lin, Francisco Vega, Navin Varadarajan, Ken Chen, David Marin, Elizabeth J Shpall, Katayoun Rezvani

Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being a key component. Yet, the impact of costimulatory domains on the downstream signaling and subsequent functionality of CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated the impact of various costimulatory domains on CAR-NK cell activity, using a CD70-targeting CAR. We found that CD28, a costimulatory molecule not inherently present in mature NK cells, significantly enhanced the antitumor efficacy and long-term cytotoxicity of CAR-NK cells both in vitro and in multiple xenograft models of hematologic and solid tumors. Mechanistically, we showed that CD28 linked to CD3ζ creates a platform that recruits critical kinases, such as lymphocyte-specific protein tyrosine kinase (LCK) and zeta-chain-associated protein kinase 70 (ZAP70), initiating a signaling cascade that enhances CAR-NK cell function. Our study provides insights into how CD28 costimulation enhances CAR-NK cell function and supports its incorporation in NK-based CARs for cancer immunotherapy. Significance: We demonstrated that incorporation of the T-cell-centric costimulatory molecule CD28, which is normally absent in mature natural killer (NK) cells, into the chimeric antigen receptor (CAR) construct recruits key kinases including lymphocyte-specific protein tyrosine kinase and zeta-chain-associated protein kinase 70 and results in enhanced CAR-NK cell persistence and sustained antitumor cytotoxicity.

嵌合抗原受体(CAR)设计中的多种因素会影响 CAR T 细胞的活性,其中成本调控信号是一个关键因素。然而,成本调控域对 CAR 工程自然杀伤(NK)细胞的下游信号转导和后续功能的影响在很大程度上仍未得到探讨。在这里,我们使用一种 CD70 靶向 CAR 评估了各种成本调控域对 CAR-NK 细胞活性的影响。我们发现,CD28是成熟NK细胞中并不固有的一种协同调控分子,它能显著增强CAR-NK细胞在体外以及多种血液肿瘤和实体瘤异种移植模型中的抗肿瘤疗效和长期细胞毒性。从机理上讲,我们发现 CD28 与 CD3Z 连接形成了一个平台,它能招募 LCK 和 ZAP70 等关键激酶,启动信号级联,增强 CAR-NK 细胞的功能。我们的研究深入揭示了 CD28 成本刺激如何增强 CAR-NK 细胞功能,并支持将其纳入基于 NK 的 CAR 用于癌症免疫疗法。
{"title":"CD28 Costimulation Augments CAR Signaling in NK Cells via the LCK/CD3ζ/ZAP70 Signaling Axis.","authors":"Sunil Acharya, Rafet Basar, May Daher, Hind Rafei, Ping Li, Nadima Uprety, Emily Ensley, Mayra Shanley, Bijender Kumar, Pinaki P Banerjee, Luciana Melo Garcia, Paul Lin, Vakul Mohanty, Kun H Kim, Xianli Jiang, Yuchen Pan, Ye Li, Bin Liu, Ana K Nunez Cortes, Chenyu Zhang, Mohsen Fathi, Ali Rezvan, Melisa J Montalvo, Sophia L Cha, Francia Reyes-Silva, Rejeena Shrestha, Xingliang Guo, Kiran Kundu, Alexander Biederstädt, Luis Muniz-Feliciano, Gary M Deyter, Mecit Kaplan, Xin R Jiang, Enli Liu, Antrix Jain, Janos Roszik, Natalie W Fowlkes, Luisa M Solis Soto, Maria G Raso, Joseph D Khoury, Pei Lin, Francisco Vega, Navin Varadarajan, Ken Chen, David Marin, Elizabeth J Shpall, Katayoun Rezvani","doi":"10.1158/2159-8290.CD-24-0096","DOIUrl":"10.1158/2159-8290.CD-24-0096","url":null,"abstract":"<p><p>Multiple factors in the design of a chimeric antigen receptor (CAR) influence CAR T-cell activity, with costimulatory signals being a key component. Yet, the impact of costimulatory domains on the downstream signaling and subsequent functionality of CAR-engineered natural killer (NK) cells remains largely unexplored. Here, we evaluated the impact of various costimulatory domains on CAR-NK cell activity, using a CD70-targeting CAR. We found that CD28, a costimulatory molecule not inherently present in mature NK cells, significantly enhanced the antitumor efficacy and long-term cytotoxicity of CAR-NK cells both in vitro and in multiple xenograft models of hematologic and solid tumors. Mechanistically, we showed that CD28 linked to CD3ζ creates a platform that recruits critical kinases, such as lymphocyte-specific protein tyrosine kinase (LCK) and zeta-chain-associated protein kinase 70 (ZAP70), initiating a signaling cascade that enhances CAR-NK cell function. Our study provides insights into how CD28 costimulation enhances CAR-NK cell function and supports its incorporation in NK-based CARs for cancer immunotherapy. Significance: We demonstrated that incorporation of the T-cell-centric costimulatory molecule CD28, which is normally absent in mature natural killer (NK) cells, into the chimeric antigen receptor (CAR) construct recruits key kinases including lymphocyte-specific protein tyrosine kinase and zeta-chain-associated protein kinase 70 and results in enhanced CAR-NK cell persistence and sustained antitumor cytotoxicity.</p>","PeriodicalId":9430,"journal":{"name":"Cancer discovery","volume":" ","pages":"1879-1900"},"PeriodicalIF":29.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oncogenic KRAS-Dependent Stromal Interleukin-33 Directs the Pancreatic Microenvironment to Promote Tumor Growth. 致癌基因 KRAS 依赖性基质白细胞介素-33 引导胰腺微环境促进肿瘤生长。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-04 DOI: 10.1158/2159-8290.CD-24-0100
Katelyn L Donahue, Hannah R Watkoske, Padma Kadiyala, Wenting Du, Kristee Brown, Michael K Scales, Ahmed M Elhossiny, Carlos E Espinoza, Emily L Lasse Opsahl, Brian D Griffith, Yukang Wen, Lei Sun, Ashley Velez-Delgado, Nur M Renollet, Jacqueline Morales, Nicholas M Nedzesky, Rachael K Baliira, Rosa E Menjivar, Paola I Medina-Cabrera, Arvind Rao, Benjamin Allen, Jiaqi Shi, Timothy L Frankel, Eileen S Carpenter, Filip Bednar, Yaqing Zhang, Marina Pasca di Magliano

Pancreatic cancer is characterized by an extensive fibroinflammatory microenvironment. During carcinogenesis, normal stromal cells are converted to cytokine-high cancer-associated fibroblasts (CAF). The mechanisms underlying this conversion, including the regulation and function of fibroblast-derived cytokines, are poorly understood. Thus, efforts to therapeutically target CAFs have so far failed. Herein, we show that signals from epithelial cells expressing oncogenic KRAS-a hallmark pancreatic cancer mutation-activate fibroblast autocrine signaling, which drives the expression of the cytokine IL33. Stromal IL33 expression remains high and dependent on epithelial KRAS throughout carcinogenesis; in turn, environmental stress induces interleukin-33 (IL33) secretion. Using compartment-specific IL33 knockout mice, we observed that lack of stromal IL33 leads to profound reprogramming of multiple components of the pancreatic tumor microenvironment, including CAFs, myeloid cells, and lymphocytes. Notably, loss of stromal IL33 leads to an increase in CD8+ T-cell infiltration and activation and, ultimately, reduced tumor growth. Significance: This study provides new insights into the mechanisms underlying the programming of CAFs and shows that during this process, expression of the cytokine IL33 is induced. CAF-derived IL33 has pleiotropic effects on the tumor microenvironment, supporting its potential as a therapeutic target.

胰腺癌的特点是具有广泛的纤维炎症微环境。在癌变过程中,正常基质细胞会转化为细胞因子含量较高的癌相关成纤维细胞(CAF)。人们对这种转化的机制,包括成纤维细胞衍生细胞因子的调节和功能,还知之甚少。因此,以 CAFs 为治疗目标的努力至今仍未成功。在这里,我们发现来自表达致癌 KRAS(一种标志性的胰腺癌突变)的上皮细胞的信号激活了成纤维细胞的自分泌信号,从而驱动了细胞因子白细胞介素-33(IL-33)的表达。在整个癌变过程中,基质 IL-33 的表达仍然很高,并依赖于上皮 KRAS;反过来,环境压力也会诱导 IL-33 的分泌。我们利用特异性IL-33基因敲除小鼠观察到,基质IL-33的缺乏会导致胰腺肿瘤微环境的多种成分发生深刻的重编程,包括CAFs、髓样细胞和淋巴细胞。值得注意的是,基质 IL-33 的缺失会导致 CD8+ T 细胞浸润和活化的增加,并最终导致肿瘤生长的减少。
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引用次数: 0
TIMP1 mediates astrocyte-dependent local immunosuppression in brain metastasis acting on infiltrating CD8+ T cells. TIMP1 在脑转移中介导星形胶质细胞依赖性局部免疫抑制,作用于浸润的 CD8+ T 细胞。
IF 29.7 1区 医学 Q1 ONCOLOGY Pub Date : 2024-10-02 DOI: 10.1158/2159-8290.CD-24-0134
Neibla Priego, Ana de Pablos-Aragoneses, Maria Perea-García, Valentina Pieri, Carolina Hernandez-Oliver, Laura Alvaro-Espinosa, Andrea Rojas, Oliva Sanchez, Ariane Steindl, Eduardo Caleiras, Fernando Garcia, Santiago Garcia-Martin, Osvaldo Grana-Castro, Sandra Garcia-Mulero, Diego Serrano, Paloma Velasco-Beltran, Borja Jimenez-Lasheras, Leire Egia-Mendikute, Luise Rupp, Antonia Stammberger, Matthias Meinhardt, Anas Chaachou-Charradi, Elena Martínez-Saez, Luca Bertero, Paola Cassoni, Luca Mangherini, Alessia Pellerino, Roberta Ruda, Riccardo Soffietti, Fatima Al-Shahrour, Paul Saftig, Rebeca Sanz-Pamplona, Marc Schmitz, Stephen J Crocker, Alfonso Calvo, Asis Palazon, Renacer Group, Manuel Valiente

Immunotherapies against brain metastases have shown clinical benefits when applied to asymptomatic patients, but they are largely ineffective in symptomatic cases for unknown reasons. Here we dissect the heterogeneity in metastasis-associated astrocytes using scRNAseq and report a population that blocks the antitumoral activity of infiltrating T cells. This pro-tumoral activity is mediated by the secretion of TIMP1 from a cluster of pSTAT3+ astrocytes that acts on CD63+ CD8+ T cells to modulate their function. Using genetic and pharmacologic approaches in mouse and human brain metastasis models, we demonstrate that combining immune checkpoint blockade antibodies with the inhibition of astrocyte-mediated local immunosuppression may benefit patients with symptomatic brain metastases. We further reveal that the presence of TIMP1 in liquid biopsies provides a biomarker to select patients for this combined immunotherapy. Overall, our findings demonstrate an unexpected immunomodulatory role for astrocytes in brain metastases with clinical implications.

针对脑转移瘤的免疫疗法在应用于无症状患者时已显示出临床疗效,但在有症状的病例中却大多无效,原因不明。在这里,我们利用 scRNAseq 对转移相关星形胶质细胞的异质性进行了剖析,并报告了一个阻止浸润 T 细胞抗肿瘤活性的群体。这种促肿瘤活性是由一组 pSTAT3+ 星形胶质细胞分泌的 TIMP1 介导的,TIMP1 作用于 CD63+ CD8+ T 细胞以调节其功能。通过在小鼠和人类脑转移模型中使用基因和药理学方法,我们证明将免疫检查点阻断抗体与抑制星形胶质细胞介导的局部免疫抑制相结合,可能会使有症状的脑转移患者受益。我们进一步发现,液体活检中 TIMP1 的存在为选择接受这种联合免疫疗法的患者提供了一种生物标志物。总之,我们的研究结果表明,星形胶质细胞在脑转移瘤中发挥着意想不到的免疫调节作用,并具有临床意义。
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引用次数: 0
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