首页 > 最新文献

CARTILAGE最新文献

英文 中文
Subchondral Bone Alignment in Osteochondral Allograft Transplants for Large Oval Defects of the Medial Femoral Condyle: Comparison of Lateral versus Medial Femoral Condyle Donors. 骨软骨异体移植治疗股骨内侧髁大椭圆形缺损时的软骨下骨对位:股骨髁外侧与股骨髁内侧供体的比较。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2024-01-29 DOI: 10.1177/19476035231226218

Objective: Supply-demand mismatch of medial femoral condyle (MFC) osteochondral allografts (OCAs) remains a rate-limiting factor in the treatment of osteochondral defects of the femoral condyle. Surface contour mapping was used to determine whether a contralateral lateral femoral condyle (LFC) versus ipsilateral MFC OCA differs in the alignment of donor:native subchondral bone for large osteochondral defects of the MFC.

Design: Thirty fresh-frozen human femoral condyles were matched by tibial width into 10 groups of 3 condyles (MFC recipient, MFC donor, and LFC donor) each for 3 cartilage surgeons (90 condyles). The recipient MFC was imaged using nano-computed tomography scan. Donor oval grafts were harvested from each matched condyle and transplanted into a 17 mm × 36 mm defect created in the recipient condyle. Following the first transplant, the recipient condyle was imaged and superimposed on the native condyle nano-CT scan. The donor plug was removed and the process repeated for the other donor. Surface height deviation and circumferential step-off height deviation were compared between native and donor subchondral bone surfaces for each transplant.

Results: There was no statistically significant difference in mean subchondral bone surface deviation (LFC = 0.87 mm, MFC = 0.76 mm, P = 0.07) nor circumferential step-off height (LFC = 0.93 mm, MFC = 0.85 mm, P = 0.09) between the LFC and MFC plugs. There were no significant differences in outcomes between surgeons.

Conclusions: There were no significant differences in subchondral bone circumferential step-off or surface deviation between ipsilateral MFC and contralateral LFC oval-shaped OCAs for 17 mm × 36 mm defects of the MFC.

目的:股骨内侧髁(MFC)骨软骨异体移植(OCA)的供需不匹配仍是治疗股骨髁骨软骨缺损的一个限制因素。我们使用表面轮廓图来确定对侧股骨外侧髁(LFC)与同侧MFC OCA在MFC大骨软骨缺损的供体与本体软骨下骨的排列上是否存在差异:按胫骨宽度将30个新鲜冷冻的人类股骨髁分成10组,每组3个髁(MFC受体、MFC供体和LFC供体),供3名软骨外科医生使用(90个髁)。受体 MFC 采用纳米计算机断层扫描成像。从每个匹配的髁突上采集供体椭圆形移植物,移植到受体髁突上一个 17 mm × 36 mm 的缺损处。第一次移植后,对受体髁突进行成像,并与原生髁突纳米 CT 扫描结果叠加。取出供体塞,然后对另一供体重复上述过程。比较每次移植的原生骨和供体软骨下骨表面的高度偏差和周向台阶高度偏差:结果:LFC 和 MFC 插条的平均软骨下骨表面偏差(LFC = 0.87 mm,MFC = 0.76 mm,P = 0.07)和周向阶梯高度(LFC = 0.93 mm,MFC = 0.85 mm,P = 0.09)在统计学上没有显著差异。不同外科医生的手术结果无明显差异:结论:同侧MFC和对侧LFC椭圆形OCAs治疗17 mm × 36 mm的MFC缺损,在软骨下骨周阶梯或表面偏差方面无明显差异。
{"title":"Subchondral Bone Alignment in Osteochondral Allograft Transplants for Large Oval Defects of the Medial Femoral Condyle: Comparison of Lateral versus Medial Femoral Condyle Donors.","authors":"Kelly M R Taylor, Conor S Locke, Timothy S Mologne, William D Bugbee, John A Grant","doi":"10.1177/19476035231226218","DOIUrl":"10.1177/19476035231226218","url":null,"abstract":"<p><strong>Objective: </strong>Supply-demand mismatch of medial femoral condyle (MFC) osteochondral allografts (OCAs) remains a rate-limiting factor in the treatment of osteochondral defects of the femoral condyle. Surface contour mapping was used to determine whether a contralateral lateral femoral condyle (LFC) versus ipsilateral MFC OCA differs in the alignment of donor:native subchondral bone for large osteochondral defects of the MFC.</p><p><strong>Design: </strong>Thirty fresh-frozen human femoral condyles were matched by tibial width into 10 groups of 3 condyles (MFC recipient, MFC donor, and LFC donor) each for 3 cartilage surgeons (90 condyles). The recipient MFC was imaged using nano-computed tomography scan. Donor oval grafts were harvested from each matched condyle and transplanted into a 17 mm × 36 mm defect created in the recipient condyle. Following the first transplant, the recipient condyle was imaged and superimposed on the native condyle nano-CT scan. The donor plug was removed and the process repeated for the other donor. Surface height deviation and circumferential step-off height deviation were compared between native and donor subchondral bone surfaces for each transplant.</p><p><strong>Results: </strong>There was no statistically significant difference in mean subchondral bone surface deviation (LFC = 0.87 mm, MFC = 0.76 mm, <i>P</i> = 0.07) nor circumferential step-off height (LFC = 0.93 mm, MFC = 0.85 mm, <i>P</i> = 0.09) between the LFC and MFC plugs. There were no significant differences in outcomes between surgeons.</p><p><strong>Conclusions: </strong>There were no significant differences in subchondral bone circumferential step-off or surface deviation between ipsilateral MFC and contralateral LFC oval-shaped OCAs for 17 mm × 36 mm defects of the MFC.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"240-249"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Outcomes After Arthroscopic Treatment of Extraspinal Diffuse Idiopathic Skeletal Hyperostosis (DISH) Involving the Hip Joint. 关节镜治疗涉及髋关节的脊柱外弥漫性特发性骨质增生(DISH)后的临床效果。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2024-01-18 DOI: 10.1177/19476035231226215

Objective: The hip joint can be affected by extraspinal diffuse idiopathic skeletal hyperostosis (DISH). This study aimed to compare the clinical characteristics of hips with DISH to those with mixed-type femoroacetabular impingement symptoms (FAIS). In addition, patient-reported outcome (PRO) scores were reported among patients with DISH involving the hip joint who underwent arthroscopic treatment.

Methods: A retrospective analysis was performed using data from patients who underwent hip arthroscopy between 2017 and 2021. Patients who had a preoperative diagnosis of extraspinal DISH of the hip joint and postoperative Hip Outcome Score-Activities of Daily Living (HOS-ADL), Hip Outcome Score-Sports Subscale (HOS-SSS), International Hip Outcome Tool 12-component form (iHOT-12), modified Harris Hip Score (mHHS) and visual analog scale (VAS) for pain scores were enrolled in the study. The patients' characteristics were compared with those of a control group (1:2) consisting of mixed-type patients with FAIS. The control group was matched in terms of age, sex, body mass index (BMI), and symptom duration.

Results: Eleven hips (0.87%) with extraspinal DISH (study group) were matched to 22 FAIS hips (control group). All the patients were male. The mean age of patients was 42.0 ± 8.0 in the study group. The study group was characterized by a larger preoperative alpha angle (79.1 ± 6.8 vs 64.8 ± 9.7, P < .001), lateral center-edge angle (LCEA) (49.7 ± 6.0 vs 40.7 ± 3.2, P < .001), and postoperative LCEA (36.6 ± 3.0 vs 34.2 ± 2.0, P = .013). In addition, a higher proportion of acetabular (81.8% vs 31.8%, P = .007) and femoral head chondral lesions (45.5% vs 9.1%, P = .016). Cartilage damage has the potential to affect the prognosis of arthroscopic treatment. Nevertheless, at the final follow-up, patients with DISH experienced a significant increase in range of motion (ROM), notable enhancements in all PROs, and favorable rates of minimal clinically important difference (MCID) for the PROs.

Conclusion: The occurrence of DISH in the hip joint is considerably infrequent, characterized by hip pain and limited ROM. Despite increased alpha angle and LCEA, and more acetabular and femoral head chondral damage noted at the time hip arthroscopy, patients with DISH observed a significant improvement in ROM, notable enhancements in all PROs, and favorable rates of MCID for the PROs.

目的:髋关节可能受到脊柱外弥漫性特发性骨骼增生症(DISH)的影响。本研究旨在比较患有 DISH 的髋关节与患有混合型股骨髋臼撞击症状(FAIS)的髋关节的临床特征。此外,还报告了接受关节镜治疗的髋关节DISH患者的患者报告结果(PRO)评分:利用2017年至2021年期间接受髋关节镜手术的患者数据进行了回顾性分析。研究对象包括术前诊断为髋关节脊柱外DISH的患者,以及术后髋关节结果评分-日常生活活动(HOS-ADL)、髋关节结果评分-运动分量表(HOS-SSS)、国际髋关节结果工具12分量表(iHOT-12)、改良哈里斯髋关节评分(mHHS)和疼痛视觉模拟量表(VAS)评分的患者。这些患者的特征与由混合型 FAIS 患者组成的对照组(1:2)的特征进行了比较。对照组在年龄、性别、体重指数(BMI)和症状持续时间等方面均匹配:11个髋关节(0.87%)患有脊柱外DISH(研究组),22个髋关节患有FAIS(对照组)。所有患者均为男性。研究组患者的平均年龄为(42.0 ± 8.0)岁。研究组的特点是术前α角(79.1 ± 6.8 vs 64.8 ± 9.7,P < .001)、外侧中心边缘角(LCEA)(49.7 ± 6.0 vs 40.7 ± 3.2,P < .001)和术后 LCEA(36.6 ± 3.0 vs 34.2 ± 2.0,P = .013)较大。此外,髋臼(81.8% vs 31.8%,P = .007)和股骨头软骨损伤的比例更高(45.5% vs 9.1%,P = .016)。软骨损伤可能会影响关节镜治疗的预后。尽管如此,在最后的随访中,DISH 患者的活动范围(ROM)显著增加,所有PRO指标均有明显改善,PRO指标的最小临床重要差异(MCID)率良好:结论:髋关节 DISH 的发生率相当低,其特点是髋关节疼痛和活动度受限。尽管在髋关节镜检查时发现α角和LCEA增大,髋臼和股骨头软骨损伤增多,但DISH患者的ROM明显改善,所有PRO指标显著提高,PRO指标的最小临床重要差异(MCID)率良好。
{"title":"Clinical Outcomes After Arthroscopic Treatment of Extraspinal Diffuse Idiopathic Skeletal Hyperostosis (DISH) Involving the Hip Joint.","authors":"Fan Yang, Zhiyu Zhang, Hongjie Huang, Yan Xu, Jianquan Wang, Xiaodong Ju","doi":"10.1177/19476035231226215","DOIUrl":"10.1177/19476035231226215","url":null,"abstract":"<p><strong>Objective: </strong>The hip joint can be affected by extraspinal diffuse idiopathic skeletal hyperostosis (DISH). This study aimed to compare the clinical characteristics of hips with DISH to those with mixed-type femoroacetabular impingement symptoms (FAIS). In addition, patient-reported outcome (PRO) scores were reported among patients with DISH involving the hip joint who underwent arthroscopic treatment.</p><p><strong>Methods: </strong>A retrospective analysis was performed using data from patients who underwent hip arthroscopy between 2017 and 2021. Patients who had a preoperative diagnosis of extraspinal DISH of the hip joint and postoperative Hip Outcome Score-Activities of Daily Living (HOS-ADL), Hip Outcome Score-Sports Subscale (HOS-SSS), International Hip Outcome Tool 12-component form (iHOT-12), modified Harris Hip Score (mHHS) and visual analog scale (VAS) for pain scores were enrolled in the study. The patients' characteristics were compared with those of a control group (1:2) consisting of mixed-type patients with FAIS. The control group was matched in terms of age, sex, body mass index (BMI), and symptom duration.</p><p><strong>Results: </strong>Eleven hips (0.87%) with extraspinal DISH (study group) were matched to 22 FAIS hips (control group). All the patients were male. The mean age of patients was 42.0 ± 8.0 in the study group. The study group was characterized by a larger preoperative alpha angle (79.1 ± 6.8 vs 64.8 ± 9.7, <i>P</i> < .001), lateral center-edge angle (LCEA) (49.7 ± 6.0 vs 40.7 ± 3.2, <i>P</i> < .001), and postoperative LCEA (36.6 ± 3.0 vs 34.2 ± 2.0, <i>P</i> = .013). In addition, a higher proportion of acetabular (81.8% vs 31.8%, <i>P</i> = .007) and femoral head chondral lesions (45.5% vs 9.1%, <i>P</i> = .016). Cartilage damage has the potential to affect the prognosis of arthroscopic treatment. Nevertheless, at the final follow-up, patients with DISH experienced a significant increase in range of motion (ROM), notable enhancements in all PROs, and favorable rates of minimal clinically important difference (MCID) for the PROs.</p><p><strong>Conclusion: </strong>The occurrence of DISH in the hip joint is considerably infrequent, characterized by hip pain and limited ROM. Despite increased alpha angle and LCEA, and more acetabular and femoral head chondral damage noted at the time hip arthroscopy, patients with DISH observed a significant improvement in ROM, notable enhancements in all PROs, and favorable rates of MCID for the PROs.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"259-267"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139484503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PKC-δ Promotes IL-1β-Induced Apoptosis of Rat Chondrocytes and Via Activating JNK and P38 MAPK Pathways. PKC-δ 通过激活 JNK 和 P38 MAPK 通路促进 IL-1β 诱导的大鼠软骨细胞凋亡
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-07-25 DOI: 10.1177/19476035231181446

Objective: Protein kinase C-delta (PKC-δ) is involved in apoptosis. This study aimed to establish whether PKC-δ can further promote IL-1β-induced chondrocyte apoptosis by mediating the phosphorylation of the JNK and p38 mitogen-activated protein kinase (MAPK) signaling pathways In osteoarthritis (OA).

Methods: We employed chondrocyte staining to determine the extent of cartilage degeneration. PKC-δ and p38 signal expressions were used in the immunohistochemical (IHC) test and apoptosis was assayed at the TUNEL test in human osteoarthritic and controls. We stimulated rat cartilage cells using IL-1β (10 ng/ml)/rottlerin (10 μM) or lentivirus. To determine the apoptosis rate, we employed flow cytometry. The mRNA of both BCL2-related X (BAX) and cysteine aspartate protease 3 (caspase-3) could be measured via qRT-PCR. Western blot measured the protein levels of BAX, caspase-3, PKC-δ, p-JNK/JNK and p-p38/p38.

Results: The positive rate of PKC-δ and the apoptotic rate of chondrocytes in OA were higher than controls. The manifestation of PKC-δ was positively related to the degree of cartilage degeneration, p38 protein expression, and apoptosis rate. IL-1β exposure upregulated PKC-δ expression in chondrocytes in a dose-dependent manner. Decreasing PKC-δ expression and its phosphorylation in OA can inhibit MAPK signaling pathway activation (phosphorylation) by downregulating JNK and p38 protein phosphorylation and expression. This inhibition decreases caspase-3 and BAX levels, consequently lowering the apoptosis rate in chondrocytes.

Conclusion: PKC-δ activation by IL-1β in OA promotes chondrocyte apoptosis via activation of the JNK and p38 MAPK signal pathways, thereby promoting the OA progression.

目的蛋白激酶C-δ(PKC-δ)参与细胞凋亡。本研究旨在确定在骨关节炎(OA)中,PKC-δ是否能通过介导JNK和p38丝裂原活化蛋白激酶(MAPK)信号通路的磷酸化,进一步促进IL-1β诱导的软骨细胞凋亡:我们采用软骨细胞染色法来确定软骨退化的程度。方法:我们采用软骨细胞染色来确定软骨退化的程度,免疫组化(IHC)检测 PKC-δ 和 p38 信号的表达,TUNEL 检测人类骨关节炎患者和对照组的细胞凋亡。我们使用 IL-1β(10 ng/ml)/rottlerin(10 μM)或慢病毒刺激大鼠软骨细胞。为了确定细胞凋亡率,我们采用了流式细胞术。通过 qRT-PCR 可测定 BCL2 相关 X(BAX)和半胱氨酸天冬氨酸蛋白酶 3(caspase-3)的 mRNA。Western blot 检测了 BAX、caspase-3、PKC-δ、p-JNK/JNK 和 p-p38/p38 的蛋白水平:结果:OA 中 PKC-δ 的阳性率和软骨细胞的凋亡率均高于对照组。PKC-δ的表达与软骨变性程度、p38蛋白表达和凋亡率呈正相关。IL-1β以剂量依赖的方式上调软骨细胞中PKC-δ的表达。在 OA 中减少 PKC-δ 的表达及其磷酸化可通过下调 JNK 和 p38 蛋白的磷酸化和表达来抑制 MAPK 信号通路的激活(磷酸化)。这种抑制作用会降低 Caspase-3 和 BAX 的水平,从而降低软骨细胞的凋亡率:结论:IL-1β激活的PKC-δ在OA中通过激活JNK和p38 MAPK信号通路促进软骨细胞凋亡,从而促进OA进展。
{"title":"PKC-δ Promotes IL-1β-Induced Apoptosis of Rat Chondrocytes and Via Activating JNK and P38 MAPK Pathways.","authors":"Jinfeng Lu, Miao Yu, Jia Li","doi":"10.1177/19476035231181446","DOIUrl":"10.1177/19476035231181446","url":null,"abstract":"<p><strong>Objective: </strong>Protein kinase C-delta (PKC-δ) is involved in apoptosis. This study aimed to establish whether PKC-δ can further promote IL-1β-induced chondrocyte apoptosis by mediating the phosphorylation of the JNK and p38 mitogen-activated protein kinase (MAPK) signaling pathways In osteoarthritis (OA).</p><p><strong>Methods: </strong>We employed chondrocyte staining to determine the extent of cartilage degeneration. PKC-δ and p38 signal expressions were used in the immunohistochemical (IHC) test and apoptosis was assayed at the TUNEL test in human osteoarthritic and controls. We stimulated rat cartilage cells using IL-1β (10 ng/ml)/rottlerin (10 μM) or lentivirus. To determine the apoptosis rate, we employed flow cytometry. The mRNA of both BCL2-related X (BAX) and cysteine aspartate protease 3 (caspase-3) could be measured via qRT-PCR. Western blot measured the protein levels of BAX, caspase-3, PKC-δ, p-JNK/JNK and p-p38/p38.</p><p><strong>Results: </strong>The positive rate of PKC-δ and the apoptotic rate of chondrocytes in OA were higher than controls. The manifestation of PKC-δ was positively related to the degree of cartilage degeneration, p38 protein expression, and apoptosis rate. IL-1β exposure upregulated PKC-δ expression in chondrocytes in a dose-dependent manner. Decreasing PKC-δ expression and its phosphorylation in OA can inhibit MAPK signaling pathway activation (phosphorylation) by downregulating JNK and p38 protein phosphorylation and expression. This inhibition decreases caspase-3 and BAX levels, consequently lowering the apoptosis rate in chondrocytes.</p><p><strong>Conclusion: </strong>PKC-δ activation by IL-1β in OA promotes chondrocyte apoptosis via activation of the JNK and p38 MAPK signal pathways, thereby promoting the OA progression.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"315-327"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9870577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Disintegrin and Metalloproteinase with Thrombospondin Motifs-4 Levels in Chondrocytes of Different Morphology within Nondegenerate and Early Osteoarthritic Human Femoral Head Cartilage. 非退行性和早期骨关节炎人股骨头软骨内不同形态软骨细胞中具有血栓软骨素基因的解体蛋白酶和金属蛋白酶-4的水平
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-06-05 DOI: 10.1177/19476035231176532
{"title":"A Disintegrin and Metalloproteinase with Thrombospondin Motifs-4 Levels in Chondrocytes of Different Morphology within Nondegenerate and Early Osteoarthritic Human Femoral Head Cartilage.","authors":"Arthur O F Herren, Anish K Amin, Andrew C Hall","doi":"10.1177/19476035231176532","DOIUrl":"10.1177/19476035231176532","url":null,"abstract":"","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"278-282"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9627435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PiRNA hsa_piR_019914 Promoted Chondrocyte Anabolic Metabolism By Inhibiting LDHA-Dependent ROS Production. PiRNA hsa_piR_019914 通过抑制依赖于 LDHA 的 ROS 生成促进软骨细胞的合成代谢。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-07-11 DOI: 10.1177/19476035231181094

Objectives: Osteoarthritis (OA) is the most common joint disease. The occurrence and progression of OA are regulated by epigenetics. A large number of studies have shown the important regulatory role of noncoding RNAs in joint diseases. As the largest class of noncoding small RNAs, the importance of piRNAs in many diseases, especially cancer, has been increasingly recognized. However, few studies have explored the role of piRNAs in OA. Our study showed that hsa_piR_019914 decreased significantly in OA. This study aimed to demonstrate the role of hsa_piR_019914 as a potential biological target of OA in chondrocytes.

Design: The GEO database and bioinformatics analysis were used for a series of screenings, and the OA model using human articular chondrocytes (C28/I2 cells), SW1353 cells under inflammatory factor stimulation was used to determine that hsa_piR_019914 was significantly downregulated in OA. Overexpression or inhibition of hsa_piR_019914 in C28/I2 cells was achieved by transfecting mimics or inhibitors. The effect of hsa_piR_019914 on the biological function of chondrocytes was verified by qPCR, flow cytometry, and colony formation assays in vitro. The target gene of hsa_piR_019914, lactate dehydrogenase A (LDHA), was screened by small RNA sequencing and quantitative polymerase chain reaction (qPCR), LDHA was knocked out in C28/I2 cells by the transfection of siRNA LDHA, and the relationship between hsa_piR_019914, LDHA, and reactive oxygen species (ROS) production was verified by flow cytometry.

Results: The piRNA hsa-piR-019914 was significantly downregulated in osteoarthritis (OA). Hsa-piR-019914 reduced inflammation-mediated chondrocyte apoptosis and maintained cell proliferation and clone formation in vitro. Hsa-piR-019914 reduced the production of LDHA-dependent ROS through targeted regulation of LDHA expression, maintained chondrocyte-specific gene expression of ACAN and COL2, and inhibited the gene expression of MMP3 and MMP13.

Conclusions: Collectively, this study showed that hsa_piR_019914 was negatively correlated with the expression of LDHA, which mediates ROS production. Under the stimulation of inflammatory factors, overexpression of hsa_piR_019914 had a protective effect on chondrocytes in vitro, and the absence of hsa_piR_019914 exacerbated the negative effect of inflammation on chondrocytes. Studies on piRNAs provide new therapeutic interventions for OA.

目的:骨关节炎(OA)是最常见的关节疾病。OA 的发生和发展受表观遗传学的调控。大量研究表明,非编码 RNA 在关节疾病中起着重要的调控作用。作为最大的一类非编码小 RNA,piRNA 在许多疾病(尤其是癌症)中的重要性已被越来越多的人所认识。然而,很少有研究探讨 piRNA 在 OA 中的作用。我们的研究表明,hsa_piR_019914在OA中显著下降。本研究旨在证明 hsa_piR_019914 在软骨细胞中作为 OA 潜在生物学靶点的作用:设计:利用 GEO 数据库和生物信息学分析进行一系列筛选,并利用炎症因子刺激下的人关节软骨细胞(C28/I2 细胞)、SW1353 细胞建立 OA 模型,确定 hsa_piR_019914 在 OA 中显著下调。通过转染模拟物或抑制剂,在 C28/I2 细胞中实现了 hsa_piR_019914 的过表达或抑制。通过 qPCR、流式细胞术和体外集落形成试验验证了 hsa_piR_019914 对软骨细胞生物学功能的影响。通过小 RNA 测序和定量聚合酶链反应(qPCR)筛选了 hsa_piR_019914 的靶基因乳酸脱氢酶 A(LDHA),通过转染 siRNA LDHA 敲除了 C28/I2 细胞中的 LDHA,并通过流式细胞术验证了 hsa_piR_019914、LDHA 和活性氧(ROS)产生之间的关系:结果:piRNA hsa-piR-019914在骨关节炎(OA)中显著下调。Hsa-piR-019914 可减少炎症介导的软骨细胞凋亡,维持体外细胞增殖和克隆形成。Hsa-piR-019914通过靶向调节LDHA的表达,减少了依赖于LDHA的ROS的产生,维持了ACAN和COL2的软骨细胞特异性基因表达,并抑制了MMP3和MMP13的基因表达:综上所述,本研究表明 hsa_piR_019914 与 LDHA 的表达呈负相关,而 LDHA 是 ROS 生成的介导因子。在炎症因子的刺激下,体外过表达 hsa_piR_019914 对软骨细胞有保护作用,而缺失 hsa_piR_019914 则会加剧炎症对软骨细胞的负面影响。对 piRNA 的研究为治疗 OA 提供了新的干预手段。
{"title":"PiRNA hsa_piR_019914 Promoted Chondrocyte Anabolic Metabolism By Inhibiting LDHA-Dependent ROS Production.","authors":"YuXuan Gao, Wen Yan, Liangye Sun, XiaoLing Zhang","doi":"10.1177/19476035231181094","DOIUrl":"10.1177/19476035231181094","url":null,"abstract":"<p><strong>Objectives: </strong>Osteoarthritis (OA) is the most common joint disease. The occurrence and progression of OA are regulated by epigenetics. A large number of studies have shown the important regulatory role of noncoding RNAs in joint diseases. As the largest class of noncoding small RNAs, the importance of piRNAs in many diseases, especially cancer, has been increasingly recognized. However, few studies have explored the role of piRNAs in OA. Our study showed that hsa_piR_019914 decreased significantly in OA. This study aimed to demonstrate the role of hsa_piR_019914 as a potential biological target of OA in chondrocytes.</p><p><strong>Design: </strong>The GEO database and bioinformatics analysis were used for a series of screenings, and the OA model using human articular chondrocytes (C28/I2 cells), SW1353 cells under inflammatory factor stimulation was used to determine that hsa_piR_019914 was significantly downregulated in OA. Overexpression or inhibition of hsa_piR_019914 in C28/I2 cells was achieved by transfecting mimics or inhibitors. The effect of hsa_piR_019914 on the biological function of chondrocytes was verified by qPCR, flow cytometry, and colony formation assays in vitro. The target gene of hsa_piR_019914, lactate dehydrogenase A (LDHA), was screened by small RNA sequencing and quantitative polymerase chain reaction (qPCR), LDHA was knocked out in C28/I2 cells by the transfection of siRNA LDHA, and the relationship between hsa_piR_019914, LDHA, and reactive oxygen species (ROS) production was verified by flow cytometry.</p><p><strong>Results: </strong>The piRNA hsa-piR-019914 was significantly downregulated in osteoarthritis (OA). Hsa-piR-019914 reduced inflammation-mediated chondrocyte apoptosis and maintained cell proliferation and clone formation in vitro. Hsa-piR-019914 reduced the production of LDHA-dependent ROS through targeted regulation of LDHA expression, maintained chondrocyte-specific gene expression of ACAN and COL2, and inhibited the gene expression of MMP3 and MMP13.</p><p><strong>Conclusions: </strong>Collectively, this study showed that hsa_piR_019914 was negatively correlated with the expression of LDHA, which mediates ROS production. Under the stimulation of inflammatory factors, overexpression of hsa_piR_019914 had a protective effect on chondrocytes in vitro, and the absence of hsa_piR_019914 exacerbated the negative effect of inflammation on chondrocytes. Studies on piRNAs provide new therapeutic interventions for OA.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"303-314"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Moderate to Severe Osteoarthritis: What is the Economic Burden for Patients and the Health Care System? Insights from the "PONOS" Study. 中度至重度骨关节炎:患者和医疗保健系统的经济负担是什么?PONOS "研究的启示。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-12-13 DOI: 10.1177/19476035231196524

Objective: To assess the economic burden of moderate to severe osteoarthritis (OA) management for patients and the health care system in Greece.

Design: A noninterventional, cross-sectional, prospective, epidemiological analysis of data from the medical records of patients with moderate to severe OA, recruited in a single visit from 9 sites in Greece. Outcomes included health care resource use (direct/indirect costs) associated with this patient population.

Results: A total of 164 patients were included in the analysis: mean age was 70.5 years, and the majority of participants were females (78.7%). The presence of comorbidities was reported by 87.2% of patients, with hypertension being the most frequently reported (53.7%). Paracetamol was the most commonly used analgesic treatment (96%), followed by systemic nonsteroidal anti-inflammatory drugs (NSAIDs) (75%) and opioids (50%). The mean overall annual direct costs per patient was estimated at 1,675.3€, with approximately half incurred by the National Health Insurance Fund, whereas the mean overall annual indirect cost (absenteeism of patients and informal caregivers) was estimated at 3,501.4€. Joint replacement (JR) procedures and paid care were the major drivers of annual direct costs in this patient population (4,326.3€ and 9,360.0€, respectively).

Conclusions: This real-world analysis of direct and indirect costs confirmed the substantial economic burden imposed by moderate to severe OA to the health care system and the patients. Our findings emphasize the need for interventions to enhance disease management, to improve patients' health outcomes and reduce the global burden of OA on society.

目的:评估希腊中重度骨关节炎(OA)治疗对患者和医疗系统造成的经济负担:评估希腊中重度骨关节炎(OA)治疗给患者和医疗系统带来的经济负担:设计:对希腊 9 个医疗机构一次性招募的中重度骨关节炎患者的病历数据进行非干预性、横断面、前瞻性、流行病学分析。结果包括与该患者群体相关的医疗资源使用情况(直接/间接成本):共有 164 名患者参与分析:平均年龄为 70.5 岁,大多数参与者为女性(78.7%)。87.2%的患者报告患有合并症,其中高血压患者最多(53.7%)。最常用的镇痛疗法是扑热息痛(96%),其次是全身性非甾体抗炎药(75%)和阿片类药物(50%)。每位患者每年的平均直接费用约为 1,675.3 欧元,其中约一半由国家医疗保险基金承担,而每年的平均间接费用(患者和非正式护理人员的缺勤)约为 3,501.4 欧元。关节置换(JR)手术和有偿护理是这一患者群体每年直接费用的主要驱动因素(分别为 4326.3 欧元和 9360.0 欧元):这项对直接和间接成本的实际分析证实,中重度 OA 给医疗系统和患者带来了巨大的经济负担。我们的研究结果表明,有必要采取干预措施,加强疾病管理,改善患者的健康状况,减轻 OA 给全球社会造成的负担。
{"title":"Moderate to Severe Osteoarthritis: What is the Economic Burden for Patients and the Health Care System? Insights from the \"PONOS\" Study.","authors":"P Savvari, I Skiadas, M Barmpouni, S A Papadakis, V Psychogios, A P Pastroudis, G A Skarpas, A Tsoutsanis, A Garofalakis, G Katsifis, O D Argyropoulou, D Boumpas, D Menegas","doi":"10.1177/19476035231196524","DOIUrl":"10.1177/19476035231196524","url":null,"abstract":"<p><strong>Objective: </strong>To assess the economic burden of moderate to severe osteoarthritis (OA) management for patients and the health care system in Greece.</p><p><strong>Design: </strong>A noninterventional, cross-sectional, prospective, epidemiological analysis of data from the medical records of patients with moderate to severe OA, recruited in a single visit from 9 sites in Greece. Outcomes included health care resource use (direct/indirect costs) associated with this patient population.</p><p><strong>Results: </strong>A total of 164 patients were included in the analysis: mean age was 70.5 years, and the majority of participants were females (78.7%). The presence of comorbidities was reported by 87.2% of patients, with hypertension being the most frequently reported (53.7%). Paracetamol was the most commonly used analgesic treatment (96%), followed by systemic nonsteroidal anti-inflammatory drugs (NSAIDs) (75%) and opioids (50%). The mean overall annual direct costs per patient was estimated at 1,675.3€, with approximately half incurred by the National Health Insurance Fund, whereas the mean overall annual indirect cost (absenteeism of patients and informal caregivers) was estimated at 3,501.4€. Joint replacement (JR) procedures and paid care were the major drivers of annual direct costs in this patient population (4,326.3€ and 9,360.0€, respectively).</p><p><strong>Conclusions: </strong>This real-world analysis of direct and indirect costs confirmed the substantial economic burden imposed by moderate to severe OA to the health care system and the patients. Our findings emphasize the need for interventions to enhance disease management, to improve patients' health outcomes and reduce the global burden of OA on society.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"268-277"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138798451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postoperative Management of Osteochondral Lesions of the Ankle: A Survey Among German-Speaking Foot and Ankle Surgeons. 踝关节骨软骨病变的术后处理:德语足和踝关节外科医生的调查。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-12-03 DOI: 10.1177/19476035231213184

Objective: To assess the current treatment of osteochondral lesions of the ankle (OCLA) by German-speaking foot and ankle surgeons, focusing on the management of postoperative care and rehabilitation.

Design: A questionnaire was created by a panel of 4 experienced foot and ankle surgeons on behalf of the "Clinical Tissue Regeneration" (CTR) working group of the German Society of Orthopaedics and Trauma Surgery (DGOU), and distributed electronically to members of the CTR, participants of the German Cartilage Registry (Knorpelregister DGOU©), and members of 6 German-speaking orthopedics or sports medicine societies. Results were classified depending on the consensus within the answers (agreement ≥75% "strong tendency," 50%-74% "tendency," 25%-49% "weak tendency," <25% "no tendency").

Results: A total of 60 participants returned the questionnaire. The main results are as follows: regarding the frequency of surgical procedures for OCLA, refixation of the fragment, retrograde drilling, and bone marrow stimulation with or without using a matrix were performed by at least 75% of the surgeons and was considered a strong tendency. There was a strong tendency to stabilize the ankle (76.7%) and perform corrective osteotomies (51.7%). In total, 75.5% and 75% of the surgeons performed bone marrow stimulation with and without using a matrix, respectively. Corrective osteotomy and ankle stabilization were performed in 64.5% and 65.2% cases, respectively. Most participants included published recommendations on postoperative rehabilitation and the return to sports activities in their postoperative management. The main surgical procedures were considered the most critical factor in influencing the postoperative management by 81% of the participants (strong tendency). Adjunct surgical procedures such as corrective osteotomy and stabilization of the ankle were considered important by 67.8% of the respondents (tendency).

Conclusions: The management of OCLA varies among German-speaking foot and ankle surgeons. Therefore, guidelines remain essential to standardize the management of OCLA, to achieve improved and stable results. This survey will assist clinicians and patients with rehabilitation to return to sports after treating the ankle's cartilage injury.

目的:评价德语足踝外科医生治疗踝关节骨软骨病变(OCLA)的现状,重点介绍术后护理和康复管理。设计:由代表德国骨科与创伤外科学会(DGOU)“临床组织再生”(CTR)工作组的4名经验丰富的足部和踝关节外科医生组成的小组制作了一份问卷,并以电子方式分发给CTR成员、德国软骨登记处(knnorpelregister DGOU©)的参与者以及6个德语骨科或运动医学学会的成员。结果根据答案的一致性进行分类(一致性≥75%为“强倾向”,50%-74%为“倾向”,25%-49%为“弱倾向”。结果:共有60名参与者返回了问卷。主要结果如下:关于OCLA手术的频率,至少75%的外科医生进行了碎片再固定、逆行钻孔和骨髓刺激(有或没有使用基质),并且被认为是一种强烈的趋势。踝关节稳定(76.7%)和矫正性截骨(51.7%)的倾向较强。总的来说,75.5%和75%的外科医生分别使用和不使用基质进行骨髓刺激。矫正截骨和踝关节稳定分别占64.5%和65.2%。大多数参与者在术后管理中纳入了关于术后康复和重返体育活动的已发表建议。81%的参与者认为主要手术方式是影响术后管理的最关键因素(强倾向)。67.8%的受访者(倾向)认为辅助外科手术如矫正截骨和踝关节稳定很重要。结论:在讲德语的足部和踝关节外科医生中,OCLA的处理方法各不相同。因此,指导方针对于规范OCLA的管理,实现改进和稳定的结果仍然是必不可少的。这项调查将有助于临床医生和康复患者在治疗踝关节软骨损伤后重返运动。
{"title":"Postoperative Management of Osteochondral Lesions of the Ankle: A Survey Among German-Speaking Foot and Ankle Surgeons.","authors":"Aurich Matthias, Christoph Becher, Sarah Ettinger, Oliver Gottschalk, Daniel Guenther, Kajetan Klos, Marc-Daniel Ahrend, Daniel Körner, Christian Plaass, Markus Walther","doi":"10.1177/19476035231213184","DOIUrl":"10.1177/19476035231213184","url":null,"abstract":"<p><strong>Objective: </strong>To assess the current treatment of osteochondral lesions of the ankle (OCLA) by German-speaking foot and ankle surgeons, focusing on the management of postoperative care and rehabilitation.</p><p><strong>Design: </strong>A questionnaire was created by a panel of 4 experienced foot and ankle surgeons on behalf of the \"Clinical Tissue Regeneration\" (CTR) working group of the German Society of Orthopaedics and Trauma Surgery (DGOU), and distributed electronically to members of the CTR, participants of the German Cartilage Registry (Knorpelregister DGOU©), and members of 6 German-speaking orthopedics or sports medicine societies. Results were classified depending on the consensus within the answers (agreement ≥75% \"strong tendency,\" 50%-74% \"tendency,\" 25%-49% \"weak tendency,\" <25% \"no tendency\").</p><p><strong>Results: </strong>A total of 60 participants returned the questionnaire. The main results are as follows: regarding the frequency of surgical procedures for OCLA, refixation of the fragment, retrograde drilling, and bone marrow stimulation with or without using a matrix were performed by at least 75% of the surgeons and was considered a strong tendency. There was a strong tendency to stabilize the ankle (76.7%) and perform corrective osteotomies (51.7%). In total, 75.5% and 75% of the surgeons performed bone marrow stimulation with and without using a matrix, respectively. Corrective osteotomy and ankle stabilization were performed in 64.5% and 65.2% cases, respectively. Most participants included published recommendations on postoperative rehabilitation and the return to sports activities in their postoperative management. The main surgical procedures were considered the most critical factor in influencing the postoperative management by 81% of the participants (strong tendency). Adjunct surgical procedures such as corrective osteotomy and stabilization of the ankle were considered important by 67.8% of the respondents (tendency).</p><p><strong>Conclusions: </strong>The management of OCLA varies among German-speaking foot and ankle surgeons. Therefore, guidelines remain essential to standardize the management of OCLA, to achieve improved and stable results. This survey will assist clinicians and patients with rehabilitation to return to sports after treating the ankle's cartilage injury.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"229-239"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138476847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of Articular Cartilage Defects: A Descriptive Analysis of Clinical Characteristics and Global Trends Reported from 2001 to 2020. 关节软骨缺损的治疗:2001年至2020年报告的临床特征和全球趋势的描述性分析。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-10-18 DOI: 10.1177/19476035231205695

Purpose: To evaluate the clinical characteristics and global trends in the surgical treatment of articular cartilage defects.

Methods: Studies in English published between January 1, 2001 and December 31, 2020 were retrieved from MEDLINE, WOS, INSPEC, SCIELO, KJD, and RSCI on the "Web of Science." Patient data were extracted, including age, sex, defect location and laterality, duration of follow-up and symptoms, and body mass index (BMI). Data were further stratified according to the surgical method, lesion location, procedural type and geographical area, and time period. A comparative analysis was performed.

Results: Overall, 443 studies involving 26,854 patients (mean age, 35.25 years; men, 60.5%) were included. The mean lesion size and patient BMI were 3.51 cm2 and 25.61 kg/m2, respectively. Cartilage defects at the knees, talus, and hips affected 20,850 (77.64%), 3,983 (14.83%), and 1,425 (5.31%) patients, respectively. The numbers of patients who underwent autologous chondrocyte implantation, arthroscopic debridement/chondroplasty, osteochondral allograft (OCA), osteochondral autologous transplantation, and microfracture were 7,114 (26.49%), 5,056 (18.83%), 3,942 (14.68%), 3,766 (14.02%), and 2,835 (10.56%), respectively. European patients were the most numerous and youngest. North American patients had the largest defects. The number of patients increased from 305 in 2001 to 3,017 in 2020. In the last 5 years, the frequency of OCAs showed a greatly increasing trend.

Conclusion: Clinical characteristics and global trends in the surgical treatment of articular cartilage defects were revealed. The choice of operation should be based on the patient characteristics and defect location, size, and shape, as well as the patient's preference.

目的:评价关节软骨缺损外科治疗的临床特点和全球趋势。方法:从“科学网”上的MEDLINE、WOS、INSPEC、SCIELO、KJD和RSCI检索2001年1月1日至2020年12月31日期间发表的英文研究。提取患者数据,包括年龄、性别、缺陷位置和偏侧性、随访时间和症状以及体重指数(BMI)。根据手术方法、病变位置、手术类型和地理区域以及时间段对数据进行进一步分层。进行了比较分析。结果:总共纳入443项研究,涉及26854名患者(平均年龄35.25岁;男性60.5%)。平均病变大小和患者BMI分别为3.51cm2和25.61kg/m2。膝关节、距骨和髋关节软骨缺损分别影响20850名(77.64%)、3983名(14.83%)和1425名(5.31%)患者。接受自体软骨细胞植入、关节镜清创/软骨成形术、同种异体骨软骨移植(OCA)、自体骨软骨移植和微骨折的患者人数分别为7114人(26.49%)、5056人(18.83%)、3942人(14.68%)、3766人(14.02%)和2835人(10.56%)。欧洲患者人数最多,年龄最小。北美患者的缺陷最大。患者人数从2001年的305人增加到2020年的3017人。在过去5年中,OCA的频率呈现出大幅增加的趋势。结论:揭示了关节软骨缺损手术治疗的临床特点和全球趋势。手术的选择应基于患者的特点和缺陷的位置、大小和形状,以及患者的偏好。
{"title":"Treatment of Articular Cartilage Defects: A Descriptive Analysis of Clinical Characteristics and Global Trends Reported from 2001 to 2020.","authors":"Zhiyuan Ren, Yang Liu, Yongsheng Ma, Lingan Huang, Xueding Wang, Qitai Lin, Yugang Xing, Wenming Yang, Wangping Duan, Xiaochun Wei","doi":"10.1177/19476035231205695","DOIUrl":"10.1177/19476035231205695","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the clinical characteristics and global trends in the surgical treatment of articular cartilage defects.</p><p><strong>Methods: </strong>Studies in English published between January 1, 2001 and December 31, 2020 were retrieved from MEDLINE, WOS, INSPEC, SCIELO, KJD, and RSCI on the \"Web of Science.\" Patient data were extracted, including age, sex, defect location and laterality, duration of follow-up and symptoms, and body mass index (BMI). Data were further stratified according to the surgical method, lesion location, procedural type and geographical area, and time period. A comparative analysis was performed.</p><p><strong>Results: </strong>Overall, 443 studies involving 26,854 patients (mean age, 35.25 years; men, 60.5%) were included. The mean lesion size and patient BMI were 3.51 cm<sup>2</sup> and 25.61 kg/m<sup>2</sup>, respectively. Cartilage defects at the knees, talus, and hips affected 20,850 (77.64%), 3,983 (14.83%), and 1,425 (5.31%) patients, respectively. The numbers of patients who underwent autologous chondrocyte implantation, arthroscopic debridement/chondroplasty, osteochondral allograft (OCA), osteochondral autologous transplantation, and microfracture were 7,114 (26.49%), 5,056 (18.83%), 3,942 (14.68%), 3,766 (14.02%), and 2,835 (10.56%), respectively. European patients were the most numerous and youngest. North American patients had the largest defects. The number of patients increased from 305 in 2001 to 3,017 in 2020. In the last 5 years, the frequency of OCAs showed a greatly increasing trend.</p><p><strong>Conclusion: </strong>Clinical characteristics and global trends in the surgical treatment of articular cartilage defects were revealed. The choice of operation should be based on the patient characteristics and defect location, size, and shape, as well as the patient's preference.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"209-218"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49674666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arthroscopic Electromechanical Assessment of Human Articular Cartilage Injury Correlates with ICRS Scores. 关节镜下人体关节软骨损伤的机电评估与 ICRS 评分相关。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-09-01 Epub Date: 2023-12-06 DOI: 10.1177/19476035231216439

Purpose: This study aimed to conduct arthroscopic evaluation of cartilage electromechanical properties and establish their correlation with International Cartilage Repair Society (ICRS) grading scores.

Methods: In 18 patients, quantitative parameter (QP) measurements were taken on the weight-bearing surface of the medial femoral condyle. Adjacently, the same site was graded using ICRS scores (0-4). Electromechanical QPs for ICRS grades 0 to 3 were obtained during arthroscopy, while complete grade 4 injuries were assessed using femur cartilage-bone blocks from knee arthroplasty. The QP values for ICRS grades 0 to 2 were compared with grades 3 and 4 using Welch t test. The corresponding QP values were assigned to ICRS grades 0 to 4 and compared using Welch ANOVA (analysis of variance). Pearson's coefficient evaluated QP-ICRS grade relationship.

Results: Healthy grade 0 cartilage displayed a mean QP value of 10.5 (±2.8 SD, n = 4). The ICRS grade 1 and grade 2 injuries were associated with QP values of 12 (±0.7, n = 2) and 13.25 (±1.77, n = 2), respectively. The grade 3 defects had QP values of 20.43 (±4.84, n = 4), whereas complete grade 4 defects showed electromechanical values of 30.17 (±2.19, n = 6). Significant differences in QP values were observed between ICRS grades 0 to 2 (mean QP 11.56 ± 2.3, n = 8) and grades 3 and 4 (26.27 ± 6, n = 10; P < 0.0001). Pearson's correlation coefficient of 0.9 indicated a strong association between higher ICRS cartilage injury grades and elevated QP values (P < 0.0001).

Conclusion: Arthroscopic electromechanical QP assessment robustly correlates with ICRS scores. The QP values for ICRS grades 0 to 2 are significantly lower, compared with grades 3 and 4.

目的:本研究旨在对软骨机电特性进行关节镜评估,并确定其与国际软骨修复学会(ICRS)分级评分的相关性:方法:对18名患者的股骨内侧髁负重面进行定量参数(QP)测量。同时,使用 ICRS 评分(0-4 分)对同一部位进行分级。ICRS 0 至 3 级的机电 QP 值是在关节镜检查中获得的,而完整的 4 级损伤则是使用膝关节置换术中的股软骨-骨块进行评估的。采用韦尔奇 t 检验比较了 ICRS 0 至 2 级与 3 和 4 级的 QP 值。将相应的 QP 值分配给 ICRS 0 至 4 级,并使用 Welch ANOVA(方差分析)进行比较。皮尔逊系数评估了QP与ICRS等级的关系:结果:健康的 0 级软骨的平均 QP 值为 10.5(±2.8 SD,n = 4)。ICRS 1 级和 2 级损伤的 QP 值分别为 12(±0.7,n = 2)和 13.25(±1.77,n = 2)。3 级缺损的 QP 值为 20.43(±4.84,n = 4),而完全 4 级缺损的机电值为 30.17(±2.19,n = 6)。在 ICRS 0 至 2 级(平均 QP 11.56 ± 2.3,n = 8)与 3 和 4 级(26.27 ± 6,n = 10;P < 0.0001)之间观察到 QP 值的显著差异。皮尔逊相关系数为0.9,表明ICRS软骨损伤等级越高,QP值越高,两者之间的关联性越强(P < 0.0001):结论:关节镜机电QP评估与ICRS评分密切相关。结论:关节镜机电 QP 评估与 ICRS 评分密切相关,ICRS 0 至 2 级的 QP 值明显低于 3 和 4 级。
{"title":"Arthroscopic Electromechanical Assessment of Human Articular Cartilage Injury Correlates with ICRS Scores.","authors":"Augustinas Rimkunas, Rimtautas Gudas, Tomas Mickevicius, Justinas Maciulaitis, Mantas Malinauskas, Alfredas Smailys, Mantas Staskunas, Arvydas Usas","doi":"10.1177/19476035231216439","DOIUrl":"10.1177/19476035231216439","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to conduct arthroscopic evaluation of cartilage electromechanical properties and establish their correlation with International Cartilage Repair Society (ICRS) grading scores.</p><p><strong>Methods: </strong>In 18 patients, quantitative parameter (QP) measurements were taken on the weight-bearing surface of the medial femoral condyle. Adjacently, the same site was graded using ICRS scores (0-4). Electromechanical QPs for ICRS grades 0 to 3 were obtained during arthroscopy, while complete grade 4 injuries were assessed using femur cartilage-bone blocks from knee arthroplasty. The QP values for ICRS grades 0 to 2 were compared with grades 3 and 4 using Welch <i>t</i> test. The corresponding QP values were assigned to ICRS grades 0 to 4 and compared using Welch ANOVA (analysis of variance). Pearson's coefficient evaluated QP-ICRS grade relationship.</p><p><strong>Results: </strong>Healthy grade 0 cartilage displayed a mean QP value of 10.5 (±2.8 SD, <i>n</i> = 4). The ICRS grade 1 and grade 2 injuries were associated with QP values of 12 (±0.7, <i>n</i> = 2) and 13.25 (±1.77, <i>n</i> = 2), respectively. The grade 3 defects had QP values of 20.43 (±4.84, <i>n</i> = 4), whereas complete grade 4 defects showed electromechanical values of 30.17 (±2.19, <i>n</i> = 6). Significant differences in QP values were observed between ICRS grades 0 to 2 (mean QP 11.56 ± 2.3, <i>n</i> = 8) and grades 3 and 4 (26.27 ± 6, <i>n</i> = 10; <i>P</i> < 0.0001). Pearson's correlation coefficient of 0.9 indicated a strong association between higher ICRS cartilage injury grades and elevated QP values (<i>P</i> < 0.0001).</p><p><strong>Conclusion: </strong>Arthroscopic electromechanical QP assessment robustly correlates with ICRS scores. The QP values for ICRS grades 0 to 2 are significantly lower, compared with grades 3 and 4.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"250-258"},"PeriodicalIF":2.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138486818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Literature that Commercial Insurance Payers Use to Substantiate Knee Osteochondral Allograft Policies Are of a Low Level of Evidence. 商业保险支付方用于证明膝关节骨软骨异体移植政策的文献证据水平较低。
IF 2.7 4区 医学 Q1 ORTHOPEDICS Pub Date : 2024-08-30 DOI: 10.1177/19476035241276859
Amir Fathi, Jacob L Kotlier, Sahil S Telang, Vishal S Patel, Ioanna K Bolia, Brett M Biedermann, Christian A Cruz, Eric H Lin, Frank A Petrigliano, Joseph N Liu

Objective: The purpose of this study is to analyze how the largest insurance companies support their medical necessity policies regarding osteochondral allograft transplantation (OCA) and to determine whether the literature they cite in their policies is of a high level of evidence (LOE).

Design: The 10 largest national health insurance companies were identified. Each payer was contacted via phone or email to obtain their coverage policy regarding OCA. For each policy, the medical necessity criteria were recorded, and all cited references were screened. For all references applicable to OCA, the LOE was recorded, and each reference was screened to determine whether they mentioned the specific criteria reported in the policies.

Results: The medical policies for 6 of the 10 national health insurance companies were identified. These 6 policies cited a collective total of 102 applicable references. Most of these studies were an LOE of IV (n = 58, 56.9%) and an LOE of V (n = 18, 17.6%). There were similarities amongst the medical necessity criteria between different commercial payers; however, most criteria were poorly supported by the cited literature.

Conclusions: Our results demonstrate that commercial insurance companies utilize studies that are of a low LOE when justifying their medical necessity criteria. Moreover, these cited studies infrequently support or mention the commercial payers' criteria. Future studies should continue to explore how well-supported insurance policies are with the goal of potentially increasing access and authorization for well-supported treatment modalities.

研究目的本研究旨在分析最大的保险公司如何支持其有关骨软骨异体移植(OCA)的医疗必要性政策,并确定其政策中引用的文献是否具有高水平的证据(LOE):设计:确定了 10 家最大的全国性医疗保险公司。设计:确定了 10 家最大的全国性医疗保险公司,并通过电话或电子邮件与每家保险公司取得联系,以了解其有关 OCA 的承保政策。记录每项政策的医疗必要性标准,并筛选所有引用的参考文献。对于所有适用于 OCA 的参考文献,均记录了 LOE,并对每个参考文献进行了筛选,以确定它们是否提到了政策中报告的特定标准:结果:确定了 10 家全国性医疗保险公司中 6 家公司的医疗政策。结果:在 10 家全国性医疗保险公司中,确定了 6 家公司的医疗政策,这 6 项政策共引用了 102 篇相关参考文献。其中大部分研究的LOE为IV(58份,占56.9%),LOE为V(18份,占17.6%)。不同商业支付方的医疗必要性标准有相似之处;但是,大多数标准都缺乏引用文献的支持:我们的研究结果表明,商业保险公司在论证其医疗必要性标准时,会使用LOE较低的研究。此外,这些被引用的研究很少支持或提及商业保险公司的标准。未来的研究应继续探讨保险政策的支持程度,目的是增加对支持程度高的治疗方式的使用和授权。
{"title":"The Literature that Commercial Insurance Payers Use to Substantiate Knee Osteochondral Allograft Policies Are of a Low Level of Evidence.","authors":"Amir Fathi, Jacob L Kotlier, Sahil S Telang, Vishal S Patel, Ioanna K Bolia, Brett M Biedermann, Christian A Cruz, Eric H Lin, Frank A Petrigliano, Joseph N Liu","doi":"10.1177/19476035241276859","DOIUrl":"10.1177/19476035241276859","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study is to analyze how the largest insurance companies support their medical necessity policies regarding osteochondral allograft transplantation (OCA) and to determine whether the literature they cite in their policies is of a high level of evidence (LOE).</p><p><strong>Design: </strong>The 10 largest national health insurance companies were identified. Each payer was contacted via phone or email to obtain their coverage policy regarding OCA. For each policy, the medical necessity criteria were recorded, and all cited references were screened. For all references applicable to OCA, the LOE was recorded, and each reference was screened to determine whether they mentioned the specific criteria reported in the policies.</p><p><strong>Results: </strong>The medical policies for 6 of the 10 national health insurance companies were identified. These 6 policies cited a collective total of 102 applicable references. Most of these studies were an LOE of IV (<i>n</i> = 58, 56.9%) and an LOE of V (<i>n</i> = 18, 17.6%). There were similarities amongst the medical necessity criteria between different commercial payers; however, most criteria were poorly supported by the cited literature.</p><p><strong>Conclusions: </strong>Our results demonstrate that commercial insurance companies utilize studies that are of a low LOE when justifying their medical necessity criteria. Moreover, these cited studies infrequently support or mention the commercial payers' criteria. Future studies should continue to explore how well-supported insurance policies are with the goal of potentially increasing access and authorization for well-supported treatment modalities.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241276859"},"PeriodicalIF":2.7,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
CARTILAGE
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1