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The Interplay Between Endoplasmic Reticulum Stress and Oxidative Stress in Chondrocyte Catabolism 软骨细胞分解过程中内质网应激与氧化应激的相互作用
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-04-20 DOI: 10.1177/19476035241245803
Yu Jung Kim, Jin Han, Seungwoo Han
ObjectiveOxidative stress and endoplasmic reticulum (ER) stress play pivotal roles in disrupting the homeostasis of chondrocytes by producing catalytic proteases and enhancing chondrocyte senescence, consequently contributing to the progression of osteoarthritis (OA). Despite their close interaction, the underlying molecular mechanisms remain poorly understood. Here, we show that ER stress and oxidative stress reciprocally modulate each other to promote cartilage degradation.MethodsPrimary chondrocytes were obtained from the articular cartilage of 5-day-old C57BL/6J mice by excising distal femur and proximal tibia. Tunicamycin was applied to induce ER stress in primary chondrocytes. Surgical OA was induced in 12-week-old male C57BL/6J mice by destabilizing the medial meniscus (DMM).ResultsTunicamycin-induced ER stress led to an increase in the production of reactive oxygen species (ROS) and catalytic proteases, including MMP13 and Adamts5, in primary chondrocytes, and it was primarily dependent on the NADPH oxidase (NOX) system. ER stress directly increased the expression of NOX2, NOX3, NOX4, and p22phox. Specifically, the protein kinase RNA-like ER kinase (PERK) pathway is involved in the expression of NOX4 and p22phox, the inositol-requiring enzyme 1 alpha (IRE1α) pathway in NOX2 and NOX3 expression, and the activating transcription factor 6 (ATF6) pathway influences NOX3 expression in chondrocytes. Conversely, inhibiting NOX function significantly reduced both ER stress sensor–related signaling and chondrocyte catabolism, thereby decelerating the progression of surgically induced OA in vivo.ConclusionsOur findings highlight the positive feedback loop between ER stress and oxidative stress in OA pathogenesis, suggesting that targeting NOX isoforms is a promising therapeutic strategy for OA.
目的氧化应激和内质网(ER)应激通过产生催化蛋白酶和促进软骨细胞衰老,在破坏软骨细胞的平衡方面发挥着关键作用,从而导致骨关节炎(OA)的进展。尽管它们之间存在密切的相互作用,但人们对其潜在的分子机制仍然知之甚少。方法通过切除 5 天大的 C57BL/6J 小鼠的股骨远端和胫骨近端,从其关节软骨中获得初级软骨细胞。使用妥卡霉素诱导原代软骨细胞产生ER应激。结果单环霉素诱导的ER应激导致原代软骨细胞中活性氧(ROS)和催化蛋白酶(包括MMP13和Adamts5)的产生增加,并且主要依赖于NADPH氧化酶(NOX)系统。ER 应激直接增加了 NOX2、NOX3、NOX4 和 p22phox 的表达。具体来说,蛋白激酶 RNA 样 ER 激酶(PERK)途径参与 NOX4 和 p22phox 的表达,肌醇需要酶 1 α(IRE1α)途径参与 NOX2 和 NOX3 的表达,激活转录因子 6(ATF6)途径影响 NOX3 在软骨细胞中的表达。相反,抑制 NOX 功能可显著减少 ER 应激传感器相关信号传导和软骨细胞分解,从而减缓手术诱导的体内 OA 的进展。
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引用次数: 0
BMSC-Derived Exosomes Attenuate Rat Osteoarthritis by Regulating Macrophage Polarization through PINK1/Parkin Signaling Pathway BMSC 衍生的外泌体通过 PINK1/Parkin 信号通路调节巨噬细胞极化,从而减轻大鼠骨关节炎
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-04-20 DOI: 10.1177/19476035241245805
Beibei Li, Enpu Shen, Zhiwen Wu, Hui Qi, Cheng’ai Wu, Danping Liu, Xu Jiang
ObjectiveExosomes derived from bone marrow mesenchymal stem cells (BMSC-Exos) may modulate the M1/M2 polarization of macrophages during osteoarthritis (OA). However, the underlying mechanisms of BMSC-Exos in this process still need to be elucidated. In this study, we explored the role of BMSC-Exos in the polarization of macrophages in vitro and the OA rats in vivo.MethodsThe effects of BMSC-Exos on RAW264.7 cells were determined, including the production of reactive oxygen species (ROS) and the protein expression of Akt, PINK1, and Parkin. We prepared an OA model by resecting the anterior cruciate ligament and medial meniscus of Sprague–Dawley (SD) rats. Hematoxylin-eosin (H&E) and safranin O-fast green staining, immunohistochemistry and immunofluorescence analyses, and the examination of interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-α), and interleukin 10 (IL-10) were performed to assess changes in cartilage and synovium.ResultsBMSC-Exos inhibited mitochondrial membrane damage, ROS production, and the protein expression of PINK1 and Parkin. Akt phosphorylation was downregulated under lipopolysaccharide (LPS) induction but significantly recovered after treatment with BMSC-Exos. BMSC-Exos alleviated cartilage damage, inhibited M1 polarization, and promoted M2 polarization in the synovium in OA rats. The expression of PINK1 and Parkin in the synovium and the levels of IL-6, IL-1β, and TNF-α in the serum decreased, but the level of IL-10 increased when BMSC-Exos were used in OA rats.ConclusionBMSC-Exos ameliorate OA development by regulating synovial macrophage polarization, and one of the underlying mechanisms may be through inhibiting PINK1/Parkin signaling.
目的 从骨髓间充质干细胞(BMSC-Exos)中提取的小体可能会调节骨关节炎(OA)期间巨噬细胞的M1/M2极化。然而,BMSC-Exos在这一过程中的内在机制仍有待阐明。方法测定 BMSC-Exos 对 RAW264.7 细胞的影响,包括活性氧(ROS)的产生以及 Akt、PINK1 和 Parkin 蛋白的表达。我们通过切除 Sprague-Dawley (SD)大鼠的前十字韧带和内侧半月板制备了 OA 模型。通过血红素-伊红(H&E)和黄绿素-O-快绿染色、免疫组织化学和免疫荧光分析,以及白细胞介素6(IL-6)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)的检测来评估软骨和滑膜的变化。结果BMSC-Exos抑制了线粒体膜损伤、ROS产生以及PINK1和Parkin的蛋白表达。在脂多糖(LPS)诱导下,Akt磷酸化下调,但经BMSC-Exos处理后,Akt磷酸化明显恢复。BMSC-Exos 可减轻 OA 大鼠滑膜的软骨损伤,抑制 M1 极化,促进 M2 极化。结论 BMSC-Exos 通过调节滑膜巨噬细胞的极化来改善 OA 的发展,其潜在机制之一可能是通过抑制 PINK1/Parkin 信号传导。
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引用次数: 0
Faster Bone Gap Union in Medial Opening Wedge High Tibial Osteotomy With 3D-Printed Synthetic Bioresorbable Polycaprolactone and Tricalcium Phosphate Osteotomy Gap Fillers Compared to Allogeneic Osteotomy Gap Fillers: A Retrospective Matched-Pair Cohort Study 使用三维打印合成生物可吸收聚己内酯和磷酸三钙截骨间隙填充物与同种异体截骨间隙填充物相比,内侧开口楔形高胫骨截骨术的骨间隙结合更快:回顾性配对队列研究
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-04-16 DOI: 10.1177/19476035241246609
Shaun Kai Kiat Chua, Walter-Soon-Yaw Wong, Don Thong Siang Koh, Rehena Sultana, Junwei Soong, Kong Hwee Lee, Hamid Rahmatullah Bin Abdul Razak
ObjectiveThe use of synthetic bone substitute material (BSM) as osteotomy gap fillers have been reported to improve outcomes in medial opening wedge high tibial osteotomy (MOWHTO). This study aims to evaluate the early radiological outcomes (bone union) and complication rates of the novel patient-specific 3D-printed honeycomb-structured polycaprolactone and tricalcium phosphate (PCL-TCP) synthetic graft compared to allogeneic bone grafts as an osteotomy gap filler in MOWHTO.MethodsA retrospective matched-pair analysis of patients who underwent MOWHTO with either PCL-TCP synthetic graft or allogenic femoral head allograft as osteotomy gap filler was performed. The osteotomy gap was split into equal zones (Zone 1-5), and bone union was evaluated on anteroposterior radiographs based on the van Hemert classification at 1, 3, 6, and 12 months postoperatively. Postoperative complications including infection, lateral hinge fractures, and persistent pain was measured. The study and control group were matched for age, smoking status, diabetes mellitus, and osteotomy gap size.ResultsSignificantly greater bone union progression was observed in the PCL-TCP group than in the allograft group at 1 month (Zones 1-3), 3 months (Zones 1-4), 6 months (Zones 1-2, 4), and 12 months (Zones 2-3, 5) postoperatively ( P < 0.05). No significant difference in complications rates was noted between the two groups at 1 year.ConclusionsBone union rates observed in patients who underwent MOWHTO with the PCL-TCP synthetic graft osteotomy gap filler were superior to those in the allograft group at 1 year postoperatively, with no significant difference in complication rates (postoperative infection, lateral hinge fractures, and persistent pain).
目的有报道称,使用合成骨替代材料(BSM)作为截骨间隙填充物可改善内侧开口楔形高胫骨截骨术(MOWHTO)的疗效。本研究旨在评估新型患者特异性三维打印蜂窝结构聚己内酯和磷酸三钙(PCL-TCP)合成移植物与同种异体骨移植物相比,作为 MOWHTO 截骨间隙填充物的早期放射学结果(骨结合)和并发症发生率。方法 对使用 PCL-TCP 合成移植物或异体股骨头同种异体骨移植物作为截骨间隙填充物进行 MOWHTO 的患者进行回顾性配对分析。截骨间隙被分割成相等的区域(1-5 区),并在术后 1、3、6 和 12 个月根据 van Hemert 分级法在正位X光片上评估骨结合情况。对术后并发症(包括感染、侧铰链骨折和持续疼痛)进行了测量。研究组和对照组在年龄、吸烟状况、糖尿病和截骨间隙大小等方面均匹配。结果在术后 1 个月(1-3 区)、3 个月(1-4 区)、6 个月(1-2 区、4 区)和 12 个月(2-3 区、5 区),观察到 PCL-TCP 组的骨结合进展明显高于同种异体移植组(P < 0.05)。结论使用 PCL-TCP 合成移植物截骨间隙填充物进行 MOWHTO 的患者术后 1 年的骨结合率优于同种异体移植物组,并发症发生率(术后感染、侧铰链骨折和持续疼痛)无明显差异。
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引用次数: 0
Expression of Chondrogenic Potential Markers in Cultured Chondrocytes from the Human Knee Joint 人膝关节培养软骨细胞中软骨生成潜能标志物的表达
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-04-15 DOI: 10.1177/19476035241241930
John-Peter Bonello, M. Yat Tse, Trevor J. G. Robinson, Davide D. Bardana, Stephen D. Waldman, Stephen C. Pang
ObjectivesWhile substantial progress has been made in engineering cartilaginous constructs for animal models, further research is needed to translate these methodologies for human applications. Evidence suggests that cultured autologous chondrocytes undergo changes in phenotype and gene expression, thereby affecting their proliferation and differentiation capacity. This study was designed to evaluate the expression of chondrogenic markers in cultured human articular chondrocytes from passages 3 (P3) and 7 (P7), beyond the current clinical recommendation of P3.MethodsCultured autologous chondrocytes were passaged from P3 up to P7, and quantitative polymerase chain reaction (qPCR) was used to assess mRNA expression of chondrogenic markers, including collagen type I (COLI), collagen type II (COLII), aggrecan (AGG), bone morphogenetic protein 4 (BMP4), transcription factor SOX-9 (SOX9), proteoglycan 4 (PGR4), and transformation-related protein 53 (p53), between P3 and P7.ResultsExcept for AGG, no significant differences were found in the expression of markers between passages, suggesting the maintenance of chondrogenic potential in cultured chondrocytes. Differential expression identified between SOX9 and PGR4, as well as between COLI and SOX9, indicates that differences in chondrogenic markers are present between age groups and sexes, respectively.ConclusionsOverall, expression profiles of younger and male chondrocytes exhibit conversion of mature cartilage characteristics compared to their counterparts, with signs of dedifferentiation and loss of phenotype within-group passaging. These results may have implications in guiding the use of higher passaged chondrocytes for engineering constructs and provide a foundation for clinical recommendations surrounding the repair and treatment of articular cartilage pathology in both sexes.
目的虽然在动物模型软骨构建工程方面取得了重大进展,但要将这些方法转化为人类应用还需要进一步的研究。有证据表明,培养的自体软骨细胞的表型和基因表达会发生变化,从而影响其增殖和分化能力。本研究旨在评估培养的人关节软骨细胞在第 3 和第 7 周期(超过目前临床推荐的第 3 周期)的软骨生成标志物表达情况。方法将培养的自体软骨细胞从 P3 培养到 P7,并使用定量聚合酶链反应(qPCR)评估 P3 和 P7 之间软骨生成标志物 mRNA 的表达,包括 I 型胶原(COLI)、II 型胶原(COLII)、凝集素(AGG)、骨形态发生蛋白 4(BMP4)、转录因子 SOX-9(SOX9)、蛋白聚糖 4(PGR4)和转化相关蛋白 53(p53)。结果除 AGG 外,不同培养期的标记物表达无显著差异,表明培养的软骨细胞保持了软骨生成潜能。SOX9和PGR4之间以及COLI和SOX9之间的表达差异表明,不同年龄组和性别之间的软骨源标记物存在差异。结论总体而言,与同龄人相比,年轻和男性软骨细胞的表达谱显示出成熟软骨特征的转换,在组内传代过程中出现了去分化和表型丧失的迹象。这些结果可能有助于指导将高传代软骨细胞用于工程构建,并为修复和治疗男女性关节软骨病变的临床建议奠定基础。
{"title":"Expression of Chondrogenic Potential Markers in Cultured Chondrocytes from the Human Knee Joint","authors":"John-Peter Bonello, M. Yat Tse, Trevor J. G. Robinson, Davide D. Bardana, Stephen D. Waldman, Stephen C. Pang","doi":"10.1177/19476035241241930","DOIUrl":"https://doi.org/10.1177/19476035241241930","url":null,"abstract":"ObjectivesWhile substantial progress has been made in engineering cartilaginous constructs for animal models, further research is needed to translate these methodologies for human applications. Evidence suggests that cultured autologous chondrocytes undergo changes in phenotype and gene expression, thereby affecting their proliferation and differentiation capacity. This study was designed to evaluate the expression of chondrogenic markers in cultured human articular chondrocytes from passages 3 (P3) and 7 (P7), beyond the current clinical recommendation of P3.MethodsCultured autologous chondrocytes were passaged from P3 up to P7, and quantitative polymerase chain reaction (qPCR) was used to assess mRNA expression of chondrogenic markers, including collagen type I (COLI), collagen type II (COLII), aggrecan (AGG), bone morphogenetic protein 4 (BMP4), transcription factor SOX-9 (SOX9), proteoglycan 4 (PGR4), and transformation-related protein 53 (p53), between P3 and P7.ResultsExcept for AGG, no significant differences were found in the expression of markers between passages, suggesting the maintenance of chondrogenic potential in cultured chondrocytes. Differential expression identified between SOX9 and PGR4, as well as between COLI and SOX9, indicates that differences in chondrogenic markers are present between age groups and sexes, respectively.ConclusionsOverall, expression profiles of younger and male chondrocytes exhibit conversion of mature cartilage characteristics compared to their counterparts, with signs of dedifferentiation and loss of phenotype within-group passaging. These results may have implications in guiding the use of higher passaged chondrocytes for engineering constructs and provide a foundation for clinical recommendations surrounding the repair and treatment of articular cartilage pathology in both sexes.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) Scores > 55 at 6 Months Postoperative Predict Ability to Achieve Patient Acceptable Symptomatic State at Minimum 1 Year Postoperative Following Autologous Chondrocyte Implantation for Grade IV Chondral Defects About the Patellofemoral Joint 软骨修复组织磁共振观察(MOCART)术后 6 个月评分 > 55 分可预测自体软骨细胞植入治疗 IV 级髌股关节软骨缺损术后至少 1 年达到患者可接受症状状态的能力
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-04-13 DOI: 10.1177/19476035241244491
Julia S. Retzky, Connor Fletcher, Morgan Rizy, Alissa Burge, Sabrina M. Strickland
PurposeThe primary aim is to evaluate the relationship between MOCART scores and patient satisfaction, as evaluated by achievement of (1) the Patient Acceptable Symptomatic State (PASS) and (2) the minimal clinically important difference (MCID) for Knee Injury and Osteoarthritis Score Quality of Life (KOOS QoL), for patients undergoing autologous chondrocyte implantation (ACI) for focal Grade IV patellofemoral chondral defects. The secondary aim is to determine the threshold MOCART score which predicts the ability to meet the PASS and the MCID for KOOS QoL.MethodsPatients undergoing ACI for grade IV patellofemoral chondral defects by a single surgeon from 2017 to 2020 were identified by search of the EMR. To determine PASS status, patients were asked, “Do you consider your current level of symptoms to be acceptable?” KOOS QoL scores were also collected. Patients with 6-month postoperative knee MRI, PASS scores, and minimum 2-year follow-up data were included. Paired t tests and Wilcoxon Rank-Sum tests were used to evaluate the relationship between MOCART scores and (1) PASS achievement and (2) achievement of the MCID for KOOS QoL (12.8).ResultsThirty-four patients were included, with a median age of 35.1 years [IQR: 24.6, 37.1], and BMI of 24.0 kg/m2 [IQR: 21.5, 28.1]. The median time to postoperative MRI was 6.7 months [IQR: 5.8, 7.9], and average follow-up time was 3.7 ± 1.2 years. Twenty-five patients (74%) achieved PASS, and 18 patients (out of 27 who had postop KOOS QoL Scores, 67%) achieved the MCID for KOOS QoL. Patients who achieved PASS had higher average MOCART scores (61.8 ± 16.0) than those who did not achieve PASS (45.0 ± 12.8, p=0.011), whereas patients who achieved the MCID for KOOS QoL did not have higher MOCART scores than those who did not achieve the MCID (61.9 ± 18.3 versus 53.3 ± 17.1, P = 0.25). There was no relationship between age, sex, lesion size, and lesion location and ability to achieve PASS or MCID for KOOS QoL (p>0.05). A threshold MOCART value of 55 was associated with the highest AUC on ROC analysis for likelihood of achieving PASS (0.778) and MCID for KOOS QoL (0.667).ConclusionHigher MOCART scores are associated with an increased likelihood of achieving PASS following patellofemoral ACI. Moreover, MOCART scores > 55 predict the ability to achieve PASS and the MCID for KOOS QoL following patellofemoral ACI.Level of Evidence:IV
目的主要目的是评估MOCART评分与患者满意度之间的关系,患者满意度的评估指标包括:(1)患者可接受症状状态(PASS)和(2)膝关节损伤和骨关节炎生活质量评分(KOOS QoL)的最小临床重要差异(MCID)。次要目的是确定预测KOOS QoL达到PASS和MCID能力的阈值MOCART评分。方法通过搜索EMR确定2017年至2020年期间由单个外科医生接受ACI治疗IV级髌骨软骨缺损的患者。为确定PASS状态,患者被问到:"您认为您目前的症状程度可以接受吗?"同时还收集了 KOOS QoL 评分。患者术后 6 个月的膝关节 MRI、PASS 评分和至少 2 年的随访数据均包括在内。采用配对t检验和Wilcoxon秩和检验来评估MOCART评分与(1)PASS得分和(2)KOOS QoL MCID (12.8)得分之间的关系。结果共纳入34名患者,中位年龄为35.1岁[IQR:24.6, 37.1],BMI为24.0 kg/m2 [IQR: 21.5, 28.1]。术后磁共振成像的中位时间为 6.7 个月 [IQR: 5.8, 7.9],平均随访时间为 3.7 ± 1.2 年。25名患者(74%)获得了PASS,18名患者(27名术后获得KOOS QoL评分的患者中,67%)的KOOS QoL达到了MCID。达到 PASS 的患者的平均 MOCART 得分(61.8 ± 16.0)高于未达到 PASS 的患者(45.0 ± 12.8,P=0.011),而达到 KOOS QoL MCID 的患者的 MOCART 得分并不比未达到 MCID 的患者高(61.9 ± 18.3 对 53.3 ± 17.1,P=0.25)。年龄、性别、病变大小和病变位置与达到 KOOS QoL PASS 或 MCID 的能力之间没有关系(P>0.05)。结论MOCART评分越高,髌骨骨关节置换术后获得PASS的可能性越大。此外,MOCART评分> 55可预测髌骨关节置换术后达到PASS的能力和KOOS QoL的MCID。
{"title":"Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) Scores > 55 at 6 Months Postoperative Predict Ability to Achieve Patient Acceptable Symptomatic State at Minimum 1 Year Postoperative Following Autologous Chondrocyte Implantation for Grade IV Chondral Defects About the Patellofemoral Joint","authors":"Julia S. Retzky, Connor Fletcher, Morgan Rizy, Alissa Burge, Sabrina M. Strickland","doi":"10.1177/19476035241244491","DOIUrl":"https://doi.org/10.1177/19476035241244491","url":null,"abstract":"PurposeThe primary aim is to evaluate the relationship between MOCART scores and patient satisfaction, as evaluated by achievement of (1) the Patient Acceptable Symptomatic State (PASS) and (2) the minimal clinically important difference (MCID) for Knee Injury and Osteoarthritis Score Quality of Life (KOOS QoL), for patients undergoing autologous chondrocyte implantation (ACI) for focal Grade IV patellofemoral chondral defects. The secondary aim is to determine the threshold MOCART score which predicts the ability to meet the PASS and the MCID for KOOS QoL.MethodsPatients undergoing ACI for grade IV patellofemoral chondral defects by a single surgeon from 2017 to 2020 were identified by search of the EMR. To determine PASS status, patients were asked, “Do you consider your current level of symptoms to be acceptable?” KOOS QoL scores were also collected. Patients with 6-month postoperative knee MRI, PASS scores, and minimum 2-year follow-up data were included. Paired t tests and Wilcoxon Rank-Sum tests were used to evaluate the relationship between MOCART scores and (1) PASS achievement and (2) achievement of the MCID for KOOS QoL (12.8).ResultsThirty-four patients were included, with a median age of 35.1 years [IQR: 24.6, 37.1], and BMI of 24.0 kg/m<jats:sup>2</jats:sup> [IQR: 21.5, 28.1]. The median time to postoperative MRI was 6.7 months [IQR: 5.8, 7.9], and average follow-up time was 3.7 ± 1.2 years. Twenty-five patients (74%) achieved PASS, and 18 patients (out of 27 who had postop KOOS QoL Scores, 67%) achieved the MCID for KOOS QoL. Patients who achieved PASS had higher average MOCART scores (61.8 ± 16.0) than those who did not achieve PASS (45.0 ± 12.8, p=0.011), whereas patients who achieved the MCID for KOOS QoL did not have higher MOCART scores than those who did not achieve the MCID (61.9 ± 18.3 versus 53.3 ± 17.1, P = 0.25). There was no relationship between age, sex, lesion size, and lesion location and ability to achieve PASS or MCID for KOOS QoL (p&gt;0.05). A threshold MOCART value of 55 was associated with the highest AUC on ROC analysis for likelihood of achieving PASS (0.778) and MCID for KOOS QoL (0.667).ConclusionHigher MOCART scores are associated with an increased likelihood of achieving PASS following patellofemoral ACI. Moreover, MOCART scores &gt; 55 predict the ability to achieve PASS and the MCID for KOOS QoL following patellofemoral ACI.Level of Evidence:IV","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significant Clinical Improvement After Arthroscopic Autologous Matrix-Induced Chondrogenesis for Osteochondral Lesions of the Talus: A 5-Year Follow-Up. 关节镜下自体基质诱导软骨生成术治疗距骨骨软骨损伤后临床症状明显改善:5 年随访。
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-03-30 DOI: 10.1177/19476035241240341
Ben Efrima, Agustin Barbero, Camilla Maccario, Cristian Indino, Chiara Nocera, Assaf Albagli, Jari Dahmen, Federico Giuseppe Usuelli

Purpose: This study aims to evaluate the clinical outcomes of arthroscopic autologous matrix-induced chondrogenesis (A-AMIC) for osteochondral lesions of the talus (OLT) at 24 months and 60 months of follow-up. The secondary aim was to assess whether age, body mass index (BMI), and lesion surface affect outcomes.

Design: Sixty-three patients (32 males, 31 females) with a median age of 37 years [interquartile range (IQR): 25-48] were included. Preoperative and postoperative (24 months and 60 months) clinical outcomes were evaluated using a Visual Analog Score (VAS) for pain during walking, the American Orthopaedic Foot and Ankle Society (AOFAS), Short-Form Survey (SF-12), the Halasi, and the University of California, Los Angeles (UCLA) scores. Patients were categorized according to age, BMI, and lesion surface (1-1.5 cm2 and over 1.5 cm2). The effect of each category was evaluated.

Results: There were significant improvements in the VAS, AOFAS, SF-12, and UCLA, comparing the preoperative scores to the 60-month follow-up scores (P < 0.001). There were no significant differences in the above-mentioned outcomes between the follow-up periods. Patients older than 33 years had lower SF-12, Halasi, and UCLA scores (P = 0.005, 0.004, and <0.001, respectively). Overweight patients had lower VAS, SF-12, Halasi, and UCLA scores (P = 0.006, 0.002, 0.024, and 0.007, respectively). Lesion size was uninfluential.

Conclusion: A-AMIC yielded clinical improvements at a minimum follow-up of 60 months in patients with symptomatic OLTs, with clinical improvement peaking in the first 2 years, followed by a plateau period. Increased age and BMI were significantly associated with inferior outcomes.

目的:本研究旨在评估关节镜下自体基质诱导软骨生成(A-AMIC)治疗距骨软骨损伤(OLT)的临床疗效,随访时间分别为24个月和60个月。次要目的是评估年龄、体重指数(BMI)和病变表面是否会影响疗效:共纳入 63 名患者(32 名男性,31 名女性),中位年龄为 37 岁[四分位数间距 (IQR):25-48]。采用行走疼痛视觉模拟评分(VAS)、美国骨科足踝协会(AOFAS)评分、短表格调查(SF-12)、Halasi评分和加州大学洛杉矶分校(UCLA)评分对术前和术后(24个月和60个月)的临床效果进行评估。根据年龄、体重指数和病变面积(1-1.5 平方厘米和 1.5 平方厘米以上)对患者进行分类。对每个类别的效果进行了评估:与术前评分和 60 个月随访评分相比,VAS、AOFAS、SF-12 和 UCLA 均有明显改善(P < 0.001)。随访期间的上述结果无明显差异。33岁以上患者的SF-12、Halasi和UCLA评分较低(分别为P = 0.005、0.004和P = 0.006、0.002、0.024和0.007)。病变大小无影响:结论:A-AMIC对有症状的OLT患者的临床改善最少随访60个月,临床改善在头两年达到高峰,随后进入平稳期。年龄和体重指数的增加与较差的治疗效果明显相关。
{"title":"Significant Clinical Improvement After Arthroscopic Autologous Matrix-Induced Chondrogenesis for Osteochondral Lesions of the Talus: A 5-Year Follow-Up.","authors":"Ben Efrima, Agustin Barbero, Camilla Maccario, Cristian Indino, Chiara Nocera, Assaf Albagli, Jari Dahmen, Federico Giuseppe Usuelli","doi":"10.1177/19476035241240341","DOIUrl":"https://doi.org/10.1177/19476035241240341","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to evaluate the clinical outcomes of arthroscopic autologous matrix-induced chondrogenesis (A-AMIC) for osteochondral lesions of the talus (OLT) at 24 months and 60 months of follow-up. The secondary aim was to assess whether age, body mass index (BMI), and lesion surface affect outcomes.</p><p><strong>Design: </strong>Sixty-three patients (32 males, 31 females) with a median age of 37 years [interquartile range (IQR): 25-48] were included. Preoperative and postoperative (24 months and 60 months) clinical outcomes were evaluated using a Visual Analog Score (VAS) for pain during walking, the American Orthopaedic Foot and Ankle Society (AOFAS), Short-Form Survey (SF-12), the Halasi, and the University of California, Los Angeles (UCLA) scores. Patients were categorized according to age, BMI, and lesion surface (1-1.5 cm<sup>2</sup> and over 1.5 cm<sup>2</sup>). The effect of each category was evaluated.</p><p><strong>Results: </strong>There were significant improvements in the VAS, AOFAS, SF-12, and UCLA, comparing the preoperative scores to the 60-month follow-up scores (<i>P</i> < 0.001). There were no significant differences in the above-mentioned outcomes between the follow-up periods. Patients older than 33 years had lower SF-12, Halasi, and UCLA scores (<i>P</i> = 0.005, 0.004, and <0.001, respectively). Overweight patients had lower VAS, SF-12, Halasi, and UCLA scores (<i>P</i> = 0.006, 0.002, 0.024, and 0.007, respectively). Lesion size was uninfluential.</p><p><strong>Conclusion: </strong>A-AMIC yielded clinical improvements at a minimum follow-up of 60 months in patients with symptomatic OLTs, with clinical improvement peaking in the first 2 years, followed by a plateau period. Increased age and BMI were significantly associated with inferior outcomes.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intra-Articular Injection of Interleukin-8 Neutralizing Monoclonal Antibody Effectively Attenuates Osteoarthritis Progression in Rabbits. 关节内注射白细胞介素-8 中和单克隆抗体可有效减缓家兔骨关节炎的发展。
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-03-25 DOI: 10.1177/19476035241240361
Junjun Yang, Xin Wang, Yingbo Zhang, Rui He, Zhenlan Fu, Rong Wang, Yanming Ma, Dejie Fu, Shuo Meng, Wang Cai, Yizhao Zhou, Cheng Chen, Guangxing Chen, Xiaoyuan Gong

Objective: Cytokines are implicated in the pathogenesis of osteoarthritis (OA), and this study aims to assess the therapeutic potential of an IL-8 neutralizing monoclonal antibody (mAb) for OA intervention.

Design: The study employed a rabbit model of OA induced by anterior cruciate ligament transection (ACLT) surgery to investigate the effects of an interleukin (IL)-8 neutralizing mAb, with hyaluronic acid (HA) used as a positive control. Primary outcomes assessed in the rabbits included cartilage repair, synovitis, joint effusion, changes in footprints, and lower limb loading conditions.

Results: Compared to HA, intra-articular injection of the IL-8 neutralizing mAb demonstrated a more pronounced attenuation of OA progression and enhancement of cartilage repair. We observed a reduction in synovitis and joint effusion, indications of bone marrow edema, as well as improvements in lower limb function. In knees treated with the neutralizing IL-8 mAb, there was a significant decrease in IL-8 levels within the synovial tissues.

Conclusions: The IL-8 neutralizing mAb exhibits promising therapeutic potential in the management of OA by attenuating inflammation and facilitating cartilage repair. However, further investigations are warranted to comprehensively elucidate the underlying mechanisms, optimize treatment protocols, and ensure the long-term safety and efficacy of this innovative therapeutic approach.

目的:细胞因子与骨关节炎(OA)的发病机制有关:细胞因子与骨关节炎(OA)的发病机制有关,本研究旨在评估IL-8中和单克隆抗体(mAb)干预OA的治疗潜力:本研究采用前十字韧带横断(ACLT)手术诱发的兔 OA 模型,研究白细胞介素(IL)-8 中和 mAb 的效果,并以透明质酸(HA)作为阳性对照。兔子的主要评估结果包括软骨修复、滑膜炎、关节积液、足印变化和下肢负荷条件:结果:与 HA 相比,关节内注射 IL-8 中和 mAb 能更明显地减缓 OA 的发展并增强软骨修复。我们还观察到滑膜炎和关节积液的减少、骨髓水肿的迹象以及下肢功能的改善。在使用中和IL-8 mAb治疗的膝关节中,滑膜组织内的IL-8水平显著下降:IL-8中和mAb可减轻炎症反应,促进软骨修复,在治疗OA方面具有广阔的前景。然而,要全面阐明其潜在机制、优化治疗方案并确保这种创新治疗方法的长期安全性和有效性,还需要进一步的研究。
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引用次数: 0
Evidence-Based Update on the Surgical Technique and Clinical Outcomes of Retrograde Drilling: A Systematic Review. 逆行钻孔手术技术和临床结果的最新循证医学证据:系统回顾
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-03-20 DOI: 10.1177/19476035241239303
Youichi Yasui, Wataru Miyamoto, Yoshiharu Shimozono, Keisuke Tsukada, Hirotaka Kawano, Masato Takao

Background: Retrograde drilling is an established surgical technique to treat osteochondral lesions of the talus (OLT). It involves non-trans-articular drilling to induce subchondral bone revascularization and bone formation without damaging the overlying articular cartilage. The present study aimed to elucidate the heterogeneity of clinical studies on retrograde drilling for OLT.

Design: A systematic search of the MEDLINE, Web of Science, EMBASE, and Cochrane Library databases for studies published between January 1996 and August 27, 2022, was performed based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines by two independent reviewers. The included studies were evaluated for their level of evidence (LoE) and quality of evidence (QoE) using the Modified Coleman Methodology Score. Variables reporting surgical and clinical outcomes and complications were evaluated.

Results: Eleven studies with 207 ankles were included (mean follow-up period = 31.1 months). The mean LoE was 3.8 (LoE 3: two studies, LoE 4: nine studies), and the mean QoE was 50.8 (fair: three studies, poor: eight studies). Ten studies used the American Orthopedic Foot and Ankle Society (AOFAS) score, which improved from 57.9 preoperatively to 86.1 postoperatively. The period and protocol of conservative treatment, lesion character, surgical technique, and postoperative protocol were inconsistent or underreported.

Conclusions: This systematic review revealed that low LoE and poor QoE, coupled with heterogeneity among the included studies, impede definitive conclusions regarding the effectiveness of this technique. Consequently, well-designed clinical trials are essential to develop standardized clinical guidelines for using retrograde drilling in OLT.

背景:逆行钻孔术是一种治疗距骨骨软骨损伤(OLT)的成熟手术技术。它通过非经关节钻孔诱导软骨下骨再血管化和骨形成,而不损伤上覆的关节软骨。本研究旨在阐明逆行钻孔治疗 OLT 的临床研究的异质性:设计:两位独立审稿人根据《系统综述和元分析首选报告项目》(Preferred Reporting Items for Systematic Reviews and Meta-Analyses,PRISMA)指南,对 MEDLINE、Web of Science、EMBASE 和 Cochrane Library 数据库中 1996 年 1 月至 2022 年 8 月 27 日期间发表的研究进行了系统检索。纳入研究的证据级别(LoE)和证据质量(QoE)采用修正科尔曼方法评分法进行评估。对报告手术和临床结果及并发症的变量进行了评估:结果:共纳入 11 项研究,涉及 207 个脚踝(平均随访时间 = 31.1 个月)。平均 LoE 为 3.8(LoE 3:2 项研究,LoE 4:9 项研究),平均 QoE 为 50.8(尚可:3 项研究,较差:8 项研究)。十项研究使用了美国骨科足踝协会(AOFAS)评分,该评分从术前的 57.9 分提高到术后的 86.1 分。保守治疗的时间和方案、病变特征、手术技术和术后方案不一致或报告不足:本系统性综述显示,LoE 低、QoE 差,再加上所纳入研究的异质性,阻碍了就该技术的有效性得出明确结论。因此,设计良好的临床试验对于制定在 OLT 中使用逆行钻孔的标准化临床指南至关重要。
{"title":"Evidence-Based Update on the Surgical Technique and Clinical Outcomes of Retrograde Drilling: A Systematic Review.","authors":"Youichi Yasui, Wataru Miyamoto, Yoshiharu Shimozono, Keisuke Tsukada, Hirotaka Kawano, Masato Takao","doi":"10.1177/19476035241239303","DOIUrl":"https://doi.org/10.1177/19476035241239303","url":null,"abstract":"<p><strong>Background: </strong>Retrograde drilling is an established surgical technique to treat osteochondral lesions of the talus (OLT). It involves non-trans-articular drilling to induce subchondral bone revascularization and bone formation without damaging the overlying articular cartilage. The present study aimed to elucidate the heterogeneity of clinical studies on retrograde drilling for OLT.</p><p><strong>Design: </strong>A systematic search of the MEDLINE, Web of Science, EMBASE, and Cochrane Library databases for studies published between January 1996 and August 27, 2022, was performed based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines by two independent reviewers. The included studies were evaluated for their level of evidence (LoE) and quality of evidence (QoE) using the Modified Coleman Methodology Score. Variables reporting surgical and clinical outcomes and complications were evaluated.</p><p><strong>Results: </strong>Eleven studies with 207 ankles were included (mean follow-up period = 31.1 months). The mean LoE was 3.8 (LoE 3: two studies, LoE 4: nine studies), and the mean QoE was 50.8 (fair: three studies, poor: eight studies). Ten studies used the American Orthopedic Foot and Ankle Society (AOFAS) score, which improved from 57.9 preoperatively to 86.1 postoperatively. The period and protocol of conservative treatment, lesion character, surgical technique, and postoperative protocol were inconsistent or underreported.</p><p><strong>Conclusions: </strong>This systematic review revealed that low LoE and poor QoE, coupled with heterogeneity among the included studies, impede definitive conclusions regarding the effectiveness of this technique. Consequently, well-designed clinical trials are essential to develop standardized clinical guidelines for using retrograde drilling in OLT.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140173830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The BMP7-Derived Peptide p[63-82] Reduces Cartilage Degeneration in the Rat ACLT-pMMx Model for Posttraumatic Osteoarthritis. BMP7衍生肽p[63-82]可减少大鼠ACLT-pMMx创伤后骨关节炎模型中的软骨退化。
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-03-19 DOI: 10.1177/19476035241233659
Ellen G J Ripmeester, Jessica S J J Steijns, Karolina A P Wijnands, Roderick H M J Stassen, Vasek Pitelka, Laura C W Peeters, Andy Cremers, Nzekui M S A Astryde, Alzbeta Chabronova, Don A M Surtel, Pieter J Emans, Guus G H van den Akker, Bert van Rietbergen, Lodewijk W van Rhijn, Marjolein M J Caron, Tim J M Welting

Objective: Osteoarthritis (OA) is characterized by articular cartilage erosion, pathological subchondral bone changes, and signs of synovial inflammation and pain. We previously identified p[63-82], a bone morphogenetic protein 7 (BMP7)-derived bioactive peptide that attenuates structural cartilage degeneration in the rat medial meniscal tear-model for posttraumatic OA. This study aimed to evaluate the cartilage erosion-attenuating activity of p[63-82] in a different preclinical model for OA (anterior cruciate ligament transection-partial medial meniscectomy [anterior cruciate ligament transection (ACLT)-pMMx]). The disease-modifying action of the p[63-82] was followed-up in this model for 5 and 10 weeks.

Design: Skeletally mature male Lewis rats underwent ACLT-pMMx surgery. Rats received weekly intra-articular injections with either saline or 500 ng p[63-82]. Five and 10 weeks postsurgery, rats were sacrificed, and subchondral bone characteristics were determined using microcomputed tomography (µCT). Histopathological evaluation of cartilage degradation and Osteoarthritis Research Society International (OARSI)-scoring was performed following Safranin-O/Fast Green staining. Pain-related behavior was measured by incapacitance testing and footprint analysis.

Results: Histopathological evaluation at 5 and 10 weeks postsurgery showed reduced cartilage degeneration and a significantly reduced OARSI score, whereas no significant changes in subchondral bone characteristics were found in the p[63-82]-treated rats compared to the saline-treated rats. ACLT-pMMx-induced imbalance of static weightbearing capacity in the p[63-82] group was significantly improved compared to the saline-treated rats at weeks 5 postsurgery. Footprint analysis scores in the p[63-82]-treated rats demonstrated improvement at week 10 postsurgery.

Conclusions: Weekly intra-articular injections of p[63-82] in the rat ACLT-pMMx posttraumatic OA model resulted in reduced degenerative cartilage changes and induced functional improvement in static weightbearing capacity during follow-up.

目的:骨关节炎(OA)的特征是关节软骨侵蚀、软骨下骨病理性改变以及滑膜炎症和疼痛。我们之前发现了一种骨形态发生蛋白 7(BMP7)衍生的生物活性肽 p[63-82],它能减轻大鼠内侧半月板撕裂模型中创伤后 OA 的软骨结构退化。本研究旨在评估 p[63-82] 在不同的 OA 临床前模型(前交叉韧带横断-部分内侧半月板切除术 [前交叉韧带横断(ACLT)-pMMx])中的软骨侵蚀抑制活性。在该模型中,对 p[63-82] 的疾病调节作用进行了 5 周和 10 周的跟踪研究:设计:骨骼发育成熟的雄性 Lewis 大鼠接受 ACLT-pMMx 手术。大鼠每周接受生理盐水或 500 ng p[63-82] 关节内注射。手术后 5 周和 10 周,大鼠被处死,并使用微计算机断层扫描(µCT)确定软骨下骨的特征。软骨退化的组织病理学评估和国际骨关节炎研究学会(OARSI)评分是在 Safranin-O/Fast Green 染色后进行的。与疼痛相关的行为通过失能测试和足迹分析进行测量:结果:术后5周和10周的组织病理学评估显示,p[63-82]处理的大鼠软骨退化程度降低,OARSI评分显著降低,而软骨下骨特征与生理盐水处理的大鼠相比没有发生显著变化。在术后第 5 周,p[63-82]组大鼠的 ACLT-pMMx 引起的静态负重能力不平衡与生理盐水治疗组相比有明显改善。p[63-82]治疗组大鼠的足印分析评分在术后第10周有所改善:结论:在大鼠 ACLT-pMMx 创伤后 OA 模型中,每周关节内注射 p[63-82] 可减少软骨退行性变化,并在随访期间改善静态负重能力。
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引用次数: 0
Hydrogel-Based Matrix-Associated Autologous Chondrocyte Implantation Shows Greater Substantial Clinical Benefit at 24 Months Follow-Up than Microfracture: A Propensity Score Matched-Pair Analysis. 基于水凝胶的基质相关自体软骨细胞植入术在 24 个月随访时比微骨折术显示出更大的实质性临床获益:倾向得分配对分析》。
IF 2.8 4区 医学 Q1 Health Professions Pub Date : 2024-03-19 DOI: 10.1177/19476035241235928
Christoph Gaissmaier, Peter Angele, Robert C Spiro, Annette Köhler, Alexandra Kirner, Philipp Niemeyer

Objective: To compare substantial clinical benefit (SCB) of a hydrogel-based, matrix-associated autologous chondrocyte implantation (M-ACI) method versus microfracture (MFx) in the treatment of knee cartilage defects.

Design: Propensity score matched-pair analysis, using the MFx control group of a phase III study as comparator for M-ACI treatment in a single-arm phase III study, resulting in 144 patients in the matched-pair set.

Results: Groups were comparable regarding baseline Knee Injury and Osteoarthritis Outcome Score (KOOS), sex, age, body mass index, symptom duration, smoking status, and previous knee surgeries. Defect sizes in the M-ACI group were significantly larger than in the MFx group (6.4 cm2 vs. 3.7 cm2). Other differences concerned location, number, and etiology of defects that were not considered to influence the interpretation of results. At 24 months, significantly more patients in the M-ACI group achieved SCB in KOOS pain (72.2% vs. 48.6%; P = 0.0108), symptoms (84.7% vs. 61.1%, P = 0.0039), sports/recreation (84.7% vs. 56.9%, P = 0.0008), and quality of life (QoL; 72.2% vs. 44.4%, P = 0.0014). The SCBs for KOOS activities in daily living and International Knee Documentation Committee score were higher for M-ACI but not significantly different from MFx. The SCB rates consistently favored M-ACI from 3 months onward. The highest improvements from baseline at 24 months in patients with SCB were observed for KOOS sports/rec. (M-ACI: 60.8 points, MFx: 55.9 points) and QoL (M-ACI: 58.1, MFx: 57.4).

Conclusion: Hydrogel-based M-ACI demonstrated superior SCB in KOOS pain, symptoms, sports/rec., and QoL compared with MFx in patients with knee cartilage defects through 2 years follow-up.

目的比较水凝胶基质相关自体软骨细胞植入术(M-ACI)与微骨折术(MFx)治疗膝关节软骨缺损的实质性临床获益(SCB):倾向得分配对分析:在一项单臂 III 期研究中,将一项 III 期研究的 MFx 对照组作为 M-ACI 治疗的比较组,结果配对组中有 144 名患者:各组在基线膝关节损伤和骨关节炎结果评分(KOOS)、性别、年龄、体重指数、症状持续时间、吸烟状况和既往膝关节手术方面具有可比性。M-ACI 组的缺损面积明显大于 MFx 组(6.4 平方厘米对 3.7 平方厘米)。其他差异涉及缺损的位置、数量和病因,但不影响对结果的解释。24 个月后,M-ACI 组患者在 KOOS 疼痛(72.2% 对 48.6%;P = 0.0108)、症状(84.7% 对 61.1%,P = 0.0039)、运动/娱乐(84.7% 对 56.9%,P = 0.0008)和生活质量(QoL;72.2% 对 44.4%,P = 0.0014)方面获得 SCB 的人数明显增多。M-ACI 在 KOOS 日常活动量和国际膝关节文献委员会评分方面的 SCB 值更高,但与 MFx 没有显著差异。从3个月起,SCB率一直倾向于M-ACI。在 24 个月时,SCB 患者的 KOOS 运动/休闲(M-ACI:60.8 分,MFx:55.9 分)和 QoL(M-ACI:58.1 分,MFx:57.4 分)与基线相比改善幅度最大:水凝胶基 M-ACI 在膝关节软骨缺损患者的 KOOS 疼痛、症状、运动/休闲和 QoL 方面的 SCB 优于 MFx(随访 2 年)。
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引用次数: 0
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